MS028269.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS028269.1 vs. NCBI nr
Match: XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])

HSP 1 Score: 678.7 bits (1750), Expect = 2.6e-191
Identity = 340/355 (95.77%), Postives = 343/355 (96.62%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHEQA VQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           SSPIMLASWHNLSARIK SDGTPFEATHGKDLWSYTAADPTHN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL GCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
           EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDAL KKTGKVI+VE VIDGNEEKN
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300

Query: 301 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 350
           LS      DMVMMAQTNKGKERT EEWAFVLHESGFSRYTITPIRAIQSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355

BLAST of MS028269.1 vs. NCBI nr
Match: XP_022135713.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 646.0 bits (1665), Expect = 1.9e-181
Identity = 318/355 (89.58%), Postives = 335/355 (94.37%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHE ARVQMWNYIFGFT++AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASN NNSMAP LLLE
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           S+P+MLA WHNLSA IK  DGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP
Sbjct: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL GCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQECVGVQHV
Sbjct: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
           EGNMFDCIPKADAAF+M VLHDWNDEDCIKILKNC+DA+ KK GKVI+VEAVIDGNEE N
Sbjct: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310

Query: 301 ------LSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 350
                 L DMVMMA TNKGKERTSEEWA+VLH++GF+RYTITP++A+QSVIQAFI
Sbjct: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365

BLAST of MS028269.1 vs. NCBI nr
Match: XP_038888665.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 538.1 bits (1385), Expect = 5.6e-149
Identity = 263/355 (74.08%), Postives = 301/355 (84.79%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD A KES  KKEE+ EQARVQ+WNYIFGF ++AI+KCAIELKIADTIE+HGS MTLSQL
Sbjct: 1   MDFAFKESIPKKEEEDEQARVQIWNYIFGFVEIAIVKCAIELKIADTIETHGSSMTLSQL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           S ALNCS  LLYRILRFL +RGIFK+E T +NLT Y+QTPLSRLLAS+ NNSM PL+LLE
Sbjct: 61  SLALNCSSSLLYRILRFLVNRGIFKQEITEENLTSYSQTPLSRLLASSNNNSMVPLVLLE 120

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           SSP+MLA WH LS RIK ++GTPFEA HGKDLWS+ AADP HN I N+AM+C ARV+TVP
Sbjct: 121 SSPVMLAPWHRLSDRIKANEGTPFEAAHGKDLWSFAAADPIHNTIINEAMSCTARVLTVP 180

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL  C EIFEG+GSLVDVGGGNGT LSMIVKACPWI+GIN DLPHV+S +QE  GVQHV
Sbjct: 181 AILEDCPEIFEGIGSLVDVGGGNGTCLSMIVKACPWIKGINLDLPHVISSSQEYTGVQHV 240

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
            G+M D IPKADAAF M VLHDW+DE CIKILKNC+DA+ +KTGKVI+VEAVI+  EE N
Sbjct: 241 SGSMLDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENN 300

Query: 301 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 350
           LS      DMVMMA TN GKERT++EW +VLH++GF+RYTITPIRA+QSVIQ F+
Sbjct: 301 LSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITPIRAVQSVIQGFL 355

BLAST of MS028269.1 vs. NCBI nr
Match: XP_023554119.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 533.5 bits (1373), Expect = 1.4e-147
Identity = 260/354 (73.45%), Postives = 302/354 (85.31%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HE AR+Q+W YIFGF +MAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SS+LNCS  LL+RI+RFL HRGIFK+E T +NLT Y+ TPLSRLL S   NSMAP +LLE
Sbjct: 61  SSSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLLE 120

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           S+P+MLA WH+LSARIKG++  PFEA HGKD+WSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDIWSYAAADPIHNAMFNDAMACSARVMSVP 180

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL  CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+GINFDLPHV++     +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVIASLPADIGVQHV 240

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
            GNMFDCIPKADAAFLM VLHDW+DE CIKILKNC+DA+ KKTGKVI+VEAV++G +E N
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 300

Query: 301 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
           LS      DMVMMA TN GKERT++EWA+VLH++GF+R+TITPIRA+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS028269.1 vs. NCBI nr
Match: XP_022952490.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 533.5 bits (1373), Expect = 1.4e-147
Identity = 264/354 (74.58%), Postives = 304/354 (85.88%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HE AR+Q+W YIFGF +MAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SS+LNCS  LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+ ++SMAPLLLLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           S+P+MLA WH LSARIKG++  PFEA HGKD+WSY AA+PTHN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL  CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ +   +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
            GNMFDCIPKADAAFLM VLH W+DE CIKILK C++A+ KKTGKVI+VEAV++  EE N
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300

Query: 301 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
           LS      DMVMMA TN GKERT+EEWA+VLH++GF+R+TITPIRA+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS028269.1 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.9e-91
Identity = 176/367 (47.96%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 5   LKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
           ++++  K  ++  +A + +W Y+FGF  +A  KCAI+LKI + IE+H S  P+TL++LSS
Sbjct: 15  MEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSS 74

Query: 65  ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TGYAQTPLSR--LLASNGNNSMAPLLLL 124
           A++ SP  L RI+RFL H+GIFKE  T   L TGY  TPLSR  ++      S+AP +L 
Sbjct: 75  AVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLF 134

Query: 125 ESSPIMLASWHNL----SARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAAR 184
           E++P MLA W  L    S+ + GS   PF+A HGKD+WS+   +P  + + N+AMAC AR
Sbjct: 135 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 194

Query: 185 VITVPAILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECV 244
            + VP + G C  +F+GV ++VDVGGG G ++ M+VK  PWI+G NFDLPHV+ VA+   
Sbjct: 195 RV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLD 254

Query: 245 GVQHVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDG 304
           GV++VEG+MFD IP  DA F+  VLHDW D+DCIKILKNCK+A+    GKV++VE+VI  
Sbjct: 255 GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGE 314

Query: 305 NEE--------------KNLSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAI 349
           N++              + + DMVMMA T+ GKERT +EW FVL E+GF+RY +  I  +
Sbjct: 315 NKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDV 374

BLAST of MS028269.1 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 5.6e-67
Identity = 146/351 (41.60%), Postives = 209/351 (59.54%), Query Frame = 0

Query: 14  EDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQLSSALNCSPP---- 73
           E+  + + ++W ++F F     +KCA+EL I D I SHG P+T+S++  +L  + P    
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 74  ---LLYRILRFLTHRGIFKEETTAQ-NLTGYAQTPLSRLLASNGNNSMAPLLLLESSPIM 133
               L RI+R L H+ +F  E   + N   Y  T  S+ L  +   +++PL+L E++PI+
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 134 LASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAILGG 193
           L  W  L  +      +PFE  HG ++W    ADP  N   N AM C+   I +  +L  
Sbjct: 125 LKPWQYL-GKCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTI-INEMLLE 184

Query: 194 CGEIFEGV-GSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHVEGNM 253
             + F G+ GSLVDVGGG G+ ++ IVKA P I+GINFDLPHVV+ A E  GV+HV G+M
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244

Query: 254 FDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDAL-SKKTGKVILVEAVI--DGNEEKN- 313
           F  IP+ADA  +  +LHDW+DEDC  ILKNC  A+  KK GKVI+V+ V+  DGN+  + 
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDK 304

Query: 314 ---LSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
              + D++MMA T  GKERT  EW  +L+ +GF RY +    A   +I+AF
Sbjct: 305 MGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353

BLAST of MS028269.1 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 7.3e-67
Identity = 136/345 (39.42%), Postives = 211/345 (61.16%), Query Frame = 0

Query: 13  EEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQLSSALNCSP---P 72
           ++D+  ++ ++WN+I+GF +  ++KCA++L +A+ I + G+ MTLS+LSS L   P    
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNED 63

Query: 73  LLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLESSPIMLASW 132
            LYR++R+L H  +F + +    L  Y   P ++ L    +  M   +L  +    +A W
Sbjct: 64  ALYRVMRYLVHMKLFTKASIDGELR-YGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPW 123

Query: 133 HNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAILGGCGEI 192
           H L   + G  GT FE   G ++W Y A  P  N++FN+AMA  +R+I + A++  CG I
Sbjct: 124 HYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-MSALVKECGNI 183

Query: 193 FEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHVEGNMFDCIP 252
           F G+ +LVDVGGG GT++  I  A P I+   +DLPHV++ +     V  V G+MF  IP
Sbjct: 184 FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 243

Query: 253 KADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKNLS------D 312
           KADA  +  +LHDW+D++CI+ILK CK+A+  K GKVI+V+ V++   E   +      D
Sbjct: 244 KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 303

Query: 313 MVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
           + MM  T  GKERT EEW  ++H++G+  + IT I A+QSVI+A+
Sbjct: 304 LDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345

BLAST of MS028269.1 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.6e-66
Identity = 129/339 (38.05%), Postives = 206/339 (60.77%), Query Frame = 0

Query: 20  RVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQLSSAL---NCSPPLLYRILR 79
           + Q+WN I+GF    +++CA+E+ IAD I+++   +TL+QL++ L   N S   LYR++R
Sbjct: 20  QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79

Query: 80  FLTHRGIFKEETTAQNLTG-YAQTPLSRLLASNGNNSMAPLLLLESSPIMLASWHNLSAR 139
           +L H  I ++ET    +   Y+  P+  LL  +   SM P++L  +    + SWH +   
Sbjct: 80  YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139

Query: 140 IKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAILGGCGEIFEGVGS 199
           +     T FE   G D+W Y   +P  +++FN+ MA   R++T   ++  C + F+G+ S
Sbjct: 140 LGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT-KTLIEDCRDTFQGLDS 199

Query: 200 LVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHVEGNMFDCIPKADAAF 259
           LVD+GGGNGT++  I +A P I+   +DLPHVV+ + +   ++ V G+MF  +P A A  
Sbjct: 200 LVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAIL 259

Query: 260 LMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKNLS------DMVMMAQ 319
           L  +LHDW DE+C+ ILK CK+A+ K+TGKVI+V+  ++      L+      D+ M+  
Sbjct: 260 LKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVN 319

Query: 320 TNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
           T  G+ERT+++W  +L  +GF  + I PIRAIQSVI+AF
Sbjct: 320 TG-GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAF 356

BLAST of MS028269.1 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.1e-66
Identity = 158/368 (42.93%), Postives = 220/368 (59.78%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           M+LA  + T    E   +    +W  I G     ++KCA+EL+I D + SH +P+TL+Q+
Sbjct: 1   MELARNDQT----EAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQI 60

Query: 61  SSALNCSPPL----LYRILRFLTHRGIFKEETTAQNLTG----YAQTPLSRLLASN---- 120
           +S++  SP L    L RI+R L  R IF +    ++L G    Y  T  SRLL S     
Sbjct: 61  ASSVPDSPSLNLSYLSRIMRLLVRRKIFSQH---KSLDGEEVLYGPTHSSRLLLSKTTLP 120

Query: 121 GNNSMAPLLLLESSPIMLASWHNLSARIKGSDGTPFEATH-GKDLWSYTAADPTHNKIFN 180
              ++AP +   + P + A W  L+  +K   G  FE  H G+ LW  +  +P  NK+FN
Sbjct: 121 DQVTLAPFVAFMTHPYLSAPWSCLARCVK-EGGNGFEMVHGGRQLWDLSPGNPEFNKVFN 180

Query: 181 DAMACAARVITVPAILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHV 240
           D MA  AR+ T+ AIL    ++F G+ SLVDVGG  G S+S IVK+ P I+GIN+DLPHV
Sbjct: 181 DGMASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHV 240

Query: 241 VSVAQECVG-VQHVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKV 300
           V+ A    G V HV GNMF+ IP A A F+  +LHDW DEDC+KILKNC+ A+ +K GK+
Sbjct: 241 VATAPTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKI 300

Query: 301 ILVEAVI--DGN----EEKNLSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRA 349
           I+V+ V+  +GN    +   + D+ +MA T +GKERT +EW  VL E GF RY I  I A
Sbjct: 301 IIVDIVLEPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPA 358

BLAST of MS028269.1 vs. ExPASy TrEMBL
Match: A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.3e-191
Identity = 340/355 (95.77%), Postives = 343/355 (96.62%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHEQA VQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           SSPIMLASWHNLSARIK SDGTPFEATHGKDLWSYTAADPTHN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL GCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
           EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDAL KKTGKVI+VE VIDGNEEKN
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300

Query: 301 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 350
           LS      DMVMMAQTNKGKERT EEWAFVLHESGFSRYTITPIRAIQSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355

BLAST of MS028269.1 vs. ExPASy TrEMBL
Match: A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 9.2e-182
Identity = 318/355 (89.58%), Postives = 335/355 (94.37%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHE ARVQMWNYIFGFT++AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASN NNSMAP LLLE
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           S+P+MLA WHNLSA IK  DGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP
Sbjct: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL GCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQECVGVQHV
Sbjct: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
           EGNMFDCIPKADAAF+M VLHDWNDEDCIKILKNC+DA+ KK GKVI+VEAVIDGNEE N
Sbjct: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310

Query: 301 ------LSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 350
                 L DMVMMA TNKGKERTSEEWA+VLH++GF+RYTITP++A+QSVIQAFI
Sbjct: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365

BLAST of MS028269.1 vs. ExPASy TrEMBL
Match: A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.7e-148
Identity = 264/354 (74.58%), Postives = 304/354 (85.88%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HE AR+Q+W YIFGF +MAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SS+LNCS  LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+ ++SMAPLLLLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           S+P+MLA WH LSARIKG++  PFEA HGKD+WSY AA+PTHN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL  CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ +   +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
            GNMFDCIPKADAAFLM VLH W+DE CIKILK C++A+ KKTGKVI+VEAV++  EE N
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300

Query: 301 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
           LS      DMVMMA TN GKERT+EEWA+VLH++GF+R+TITPIRA+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS028269.1 vs. ExPASy TrEMBL
Match: A0A6J1GLX9 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455166 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.9e-143
Identity = 258/354 (72.88%), Postives = 298/354 (84.18%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HE AR+Q+W YIFGF +MAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
           SS+LNCS  LLYRI+RFL HRGIFKEE T     GYAQTP+SRLLAS+  +SMAPLLL E
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEPT----KGYAQTPMSRLLASSSGSSMAPLLLFE 120

Query: 121 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 180
           S+P+MLA WH LSARIKG++  PFEA HGKD+WSY AA+PTHN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMSVP 180

Query: 181 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 240
           AIL  CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+ INFDLPHV++     + VQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVIASLPADIDVQHV 240

Query: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 300
            GNMFD IPKADAAFLM VLHDW+DE CIKILKNC++A+ KKTGKVI+VEAV++  EE N
Sbjct: 241 GGNMFDSIPKADAAFLMWVLHDWDDETCIKILKNCREAIPKKTGKVIIVEAVLEEKEENN 300

Query: 301 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
           LS      DMVMMA TN GKERT++EWA+VLH++GF+R+TITPIRA+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 349

BLAST of MS028269.1 vs. ExPASy TrEMBL
Match: A0A6J1GLV5 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455165 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.7e-141
Identity = 252/354 (71.19%), Postives = 297/354 (83.90%), Query Frame = 0

Query: 2   DLALKESTSK-KEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 61
           D+  K+S  K +EE++   +VQ+W YIFGF +MA+IKCAIELKI DTIE+HG  MTL+ L
Sbjct: 3   DIGSKDSKLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTLTDL 62

Query: 62  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 121
           SS+LNCSP LL+RILRFL HRGIFKEE T      YAQTP+SRLLAS+  +SMAPLLL E
Sbjct: 63  SSSLNCSPLLLHRILRFLVHRGIFKEEPT----KSYAQTPMSRLLASSSGSSMAPLLLFE 122

Query: 122 SSPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 181
           S+P+MLA WH+LSARIKG++  PFEA HGKDLWSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 123 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDLWSYAAADPIHNTMFNDAMACSARVMSVP 182

Query: 182 AILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHV 241
            +L  CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+ INFDLPHVV+ +   +GVQHV
Sbjct: 183 TLLEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVVASSPPPIGVQHV 242

Query: 242 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKN 301
            GNMFDCIPKADAAFLM VLHDW+DE CIKILKNC+DA+ KKTGKVI+VEAV++G +E N
Sbjct: 243 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 302

Query: 302 LS------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVIQAF 349
           LS      DMVMMA TN GKERT++EWA+VLH++GF+R+TITPIRA+QSVIQAF
Sbjct: 303 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRAVQSVIQAF 352

BLAST of MS028269.1 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.3e-92
Identity = 176/367 (47.96%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 5   LKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
           ++++  K  ++  +A + +W Y+FGF  +A  KCAI+LKI + IE+H S  P+TL++LSS
Sbjct: 15  MEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSS 74

Query: 65  ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TGYAQTPLSR--LLASNGNNSMAPLLLL 124
           A++ SP  L RI+RFL H+GIFKE  T   L TGY  TPLSR  ++      S+AP +L 
Sbjct: 75  AVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLF 134

Query: 125 ESSPIMLASWHNL----SARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAAR 184
           E++P MLA W  L    S+ + GS   PF+A HGKD+WS+   +P  + + N+AMAC AR
Sbjct: 135 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 194

Query: 185 VITVPAILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECV 244
            + VP + G C  +F+GV ++VDVGGG G ++ M+VK  PWI+G NFDLPHV+ VA+   
Sbjct: 195 RV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLD 254

Query: 245 GVQHVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDG 304
           GV++VEG+MFD IP  DA F+  VLHDW D+DCIKILKNCK+A+    GKV++VE+VI  
Sbjct: 255 GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGE 314

Query: 305 NEE--------------KNLSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAI 349
           N++              + + DMVMMA T+ GKERT +EW FVL E+GF+RY +  I  +
Sbjct: 315 NKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDV 374

BLAST of MS028269.1 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.4e-78
Identity = 159/363 (43.80%), Postives = 217/363 (59.78%), Query Frame = 0

Query: 5   LKESTSKKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
           ++ES     ++  +A + +W Y+FGF  +A  KCAI+LKI + IE+H S  P+TLS+LSS
Sbjct: 1   MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60

Query: 65  ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TGYAQTPLSRLLASNGNNSMAPLLLLES 124
           A++ SP  L RI+RFL H+G+FKE  T   L TGY  TPLSR +                
Sbjct: 61  AVSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM--------------- 120

Query: 125 SPIMLASWHNLSARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPA 184
                                     HGKDLW++   +  H+++ N+AMAC AR + VP 
Sbjct: 121 ----------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPR 180

Query: 185 ILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHVE 244
           + G C  +F+GV ++VDVGGG G ++ ++VK  PWI+G NFDLPHV+ VAQ   GV++VE
Sbjct: 181 VAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVE 240

Query: 245 GNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKNL 304
           G+MFD IP +DA  +  VLHDW D+DCIKILKNCK+A+    GKV++VE VI   E+KN 
Sbjct: 241 GDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVI--GEKKNT 300

Query: 305 S----------------DMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQSVI 349
                            DMVMM  T+ GKERT +EW FVL E+GF+RY +     +QS+I
Sbjct: 301 MIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 323

BLAST of MS028269.1 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 189.9 bits (481), Expect = 3.5e-48
Identity = 118/313 (37.70%), Postives = 178/313 (56.87%), Query Frame = 0

Query: 36  IKCAIELKIADTIESHGSPMTLSQLSSALNC----SPPLLYRILRFLTHRGIFKEETTAQ 95
           +K A+EL + + +  +GSPM+ ++++S L      +P +L RILR LT   +     + +
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVL--TCSNR 95

Query: 96  NLTG------YAQTPLSRLLASNGNN-SMAPLLLLESSPIMLASWHNLSARIKGSDGTPF 155
            L+G      Y   P+ + L  N +  S+A L L+    +++ SW++L   I    G PF
Sbjct: 96  KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAIL-DGGIPF 155

Query: 156 EATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAILGGCGEIFEGVGSLVDVGGGNG 215
              +G   + Y   DP  NK+FN+ M+  +  IT+  IL    + FEG+ SLVDVGGG G
Sbjct: 156 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHS-TITMKKIL-ETYKGFEGLTSLVDVGGGIG 215

Query: 216 TSLSMIVKACPWIRGINFDLPHVVSVAQECVGVQHVEGNMFDCIPKADAAFLMSVLHDWN 275
            +L MIV   P ++GINFDLPHV+  A    G++HV G+MF  +PK DA F+  + HDW+
Sbjct: 216 ATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWS 275

Query: 276 DEDCIKILKNCKDALSKKTGKVILVEAVIDGNEEKNLS-------DMVMMAQTNKGKERT 331
           DE C+K LKNC ++L  + GKVIL E ++    + +LS       D +M+A    GKERT
Sbjct: 276 DEHCVKFLKNCYESL-PEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERT 335

BLAST of MS028269.1 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 176.0 bits (445), Expect = 5.2e-44
Identity = 127/363 (34.99%), Postives = 188/363 (51.79%), Query Frame = 0

Query: 11  KKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQLSSALNC---- 70
           ++E D +   +Q  + +       ++K A EL + DTI + G+   LS    A +     
Sbjct: 21  EQEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKP 80

Query: 71  ----SPPLLYRILRFLTHRGIFK---EETTAQNLTG-----YAQTPLSR--LLASNGNNS 130
               +P LL R+L  L    I K    ET     TG     YA  P+ +  L  S+G  S
Sbjct: 81  TNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGS 140

Query: 131 MAPLLLLESSPIMLASWHNL-SARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMA 190
           + PL +L  + +   +W NL    ++G D   F + HG  ++ Y  +D    ++FN AM+
Sbjct: 141 LVPLFMLLHTQVFFKTWTNLKDVILEGRDA--FNSAHGMKIFEYINSDQPFAELFNRAMS 200

Query: 191 CAARVITVPAILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVA 250
             + +I    +    G  FE V +LVDVGGGNGT L ++    P I+G+NFDL  V++ A
Sbjct: 201 EPSTMIMKKVLDVYRG--FEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQA 260

Query: 251 QECVGVQHVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEA 310
               GV+HV G+MF  +PK DA F+  +LHDW DEDCIKILKNC  +L +K GK+I+VE 
Sbjct: 261 PFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEF 320

Query: 311 VI-----DGNEEKN---LSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQS 347
           V       G+   N     D++M+ Q + GKER+  ++  +   SGF R  I  +    S
Sbjct: 321 VTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYS 378

BLAST of MS028269.1 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 175.3 bits (443), Expect = 8.9e-44
Identity = 127/363 (34.99%), Postives = 198/363 (54.55%), Query Frame = 0

Query: 11  KKEEDHEQARVQMWNYIFGFTKMAIIKCAIELKIADTI--ESHGSPMTLSQLSSALNC-- 70
           ++E D +   +Q  + +       ++K A+EL + DTI   S+G+ ++ S+++ +L    
Sbjct: 21  EQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKP 80

Query: 71  ----SPPLLYRILRFLTHRGIFK---EETTAQNLTG-----YAQTPLSR--LLASNGNNS 130
               +P LL R+LR L    I K    E+     TG     YA  P+ +  L  S+G+ S
Sbjct: 81  TNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGS 140

Query: 131 MAPLLLLESSPIMLASWHNL-SARIKGSDGTPFEATHGKDLWSYTAADPTHNKIFNDAMA 190
           ++ LLLL  S ++L +W NL    ++G D   F + H   L+ Y ++D   +K+F+ AM+
Sbjct: 141 LSSLLLLLHSQVILKTWTNLKDVILEGKDA--FSSAHDMRLFEYISSDDQFSKLFHRAMS 200

Query: 191 CAARVITVPAILGGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVA 250
            ++ ++    +    G  FE V +LVDVGGG GT L +I    P I+G+NFDL  V++ A
Sbjct: 201 ESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQA 260

Query: 251 QECVGVQHVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALSKKTGKVILVEA 310
               GV+HV G+MF  +PK DA F+  +LHDW DEDCIKILKNC  +L +K GKVI+VE 
Sbjct: 261 PFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GKVIIVEM 320

Query: 311 VIDGNEEKN--------LSDMVMMAQTNKGKERTSEEWAFVLHESGFSRYTITPIRAIQS 347
           +     + N          D++M+ Q + GKER+  ++  +   SGF    I  +    S
Sbjct: 321 ITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYS 378