MS027627.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS027627.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	LOW QUALITY PROTEIN: major latex protein 149-like
Location	scaffold20: 1859921 .. 1860542 (+)
Sequence length	456
RNA-Seq Expression	MS027627.1
Synteny	MS027627.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAGATTTCTATCATCTCAGAGCAGGTGCAGCTCAAGTGTTCTGGTCTCAAATTCTATGAGACTTACATAAACAAATTTGGCTCTCTTCATCAAATGTTCCCTGAAAACCTTGAGAGCTACGAAATTGTGGAAGGAAATGGTTTCACTAATGGCAGCATCAGCCACTGGAAATATGACTTCGGTCAGACCCTAAACTCTAAACTCTAAATCATATACTAATGCATACTTTTCCAGTGCTTACGGTTTTCAAACGTTTTAGTTTAGTCCTTATAGTATTATACACGTTATGTTAGGAGTAGAACCATGAATTAATGTTTTTTCTTTTTGCGTTGTTCGGGAGAACTCAGGTAGCCCGACCGAGGCAAAATTAAGGCTTGTTATGGATGAGCCTAACAAATCAATAACTTTGGAGTTTCTTGAAGGAGATCTATTTAGAGACTTTGAAGTGTTTAAAGTGAAAGTTGAAGTTAGTGATGGTGGAAGGCATGGCATGAGCTCAGTTAAATGGTGCGTGGAATTTGTGAAGACAAATGAAGATGTTCCTCCACCACATAACTATCTCCAATTTGGACTTAAATTGTGCAAAGCCCTTGATGCTTACATTTGCAACAATTGA

mRNA sequence

ATGGCTCAGATTTCTATCATCTCAGAGCAGGTGCAGCTCAAGTGTTCTGGTCTCAAATTCTATGAGACTTACATAAACAAATTTGGCTCTCTTCATCAAATGTTCCCTGAAAACCTTGAGAGCTACGAAATTGTGGAAGGAAATGGTTTCACTAATGGCAGCATCAGCCACTGGAAATATGACTTCGGTAGCCCGACCGAGGCAAAATTAAGGCTTGTTATGGATGAGCCTAACAAATCAATAACTTTGGAGTTTCTTGAAGGAGATCTATTTAGAGACTTTGAAGTGTTTAAAGTGAAAGTTGAAGTTAGTGATGGTGGAAGGCATGGCATGAGCTCAGTTAAATGGTGCGTGGAATTTGTGAAGACAAATGAAGATGTTCCTCCACCACATAACTATCTCCAATTTGGACTTAAATTGTGCAAAGCCCTTGATGCTTACATTTGCAACAATTGA

Coding sequence (CDS)

ATGGCTCAGATTTCTATCATCTCAGAGCAGGTGCAGCTCAAGTGTTCTGGTCTCAAATTCTATGAGACTTACATAAACAAATTTGGCTCTCTTCATCAAATGTTCCCTGAAAACCTTGAGAGCTACGAAATTGTGGAAGGAAATGGTTTCACTAATGGCAGCATCAGCCACTGGAAATATGACTTCGGTAGCCCGACCGAGGCAAAATTAAGGCTTGTTATGGATGAGCCTAACAAATCAATAACTTTGGAGTTTCTTGAAGGAGATCTATTTAGAGACTTTGAAGTGTTTAAAGTGAAAGTTGAAGTTAGTGATGGTGGAAGGCATGGCATGAGCTCAGTTAAATGGTGCGTGGAATTTGTGAAGACAAATGAAGATGTTCCTCCACCACATAACTATCTCCAATTTGGACTTAAATTGTGCAAAGCCCTTGATGCTTACATTTGCAACAATTGA

Protein sequence

MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKYDFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTNEDVPPPHNYLQFGLKLCKALDAYICNN

Homology

[Close]

BLAST of MS027627.1 vs. NCBI nr
Match: XP_022143318.1 (LOW QUALITY PROTEIN: major latex protein 149-like [Momordica charantia])

HSP 1 Score: 298.5 bits (763), Expect = 3.2e-77
Identity = 142/148 (95.95%), Postives = 143/148 (96.62%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQISIISEQVQLKCSGLKFYETYI K GSLHQMFPENL+SYEIVEGNGFTNG ISHWKY
Sbjct: 1   MAQISIISEQVQLKCSGLKFYETYIKKIGSLHQMFPENLKSYEIVEGNGFTNGIISHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
           DFGS TEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGG HGMSSVKWCVEF
Sbjct: 61  DFGSLTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGSHGMSSVKWCVEF 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 149
           VKTNEDVPPPHNYLQFGLKLCKALDAYI
Sbjct: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 148

[Close]

BLAST of MS027627.1 vs. NCBI nr
Match: XP_008457056.1 (PREDICTED: major latex protein 15-like [Cucumis melo])

HSP 1 Score: 218.8 bits (556), Expect = 3.2e-53
Identity = 95/148 (64.19%), Postives = 122/148 (82.43%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQI  ISE+V+L C G KFY+ ++NK  S   +FP+NL+SY+ VEGNGFT+GS++HWKY
Sbjct: 1   MAQICNISEEVKLNCCGDKFYDFFLNKMDSYIHLFPQNLQSYKFVEGNGFTHGSVTHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
           DFG P E K RL++DEPNK++  E +EGDL +DFE+F+VKVEV DGG++G+SSVKW V F
Sbjct: 61  DFGIPAEVKTRLLVDEPNKAVIFECVEGDLLKDFEMFQVKVEVRDGGKNGVSSVKWSVGF 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 149
           +K NEDV PPHNYLQFG+K+CK LDAY+
Sbjct: 121 MKANEDVAPPHNYLQFGVKVCKGLDAYL 148

[Close]

BLAST of MS027627.1 vs. NCBI nr
Match: KAG6605298.1 (hypothetical protein SDJN03_02615, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 218.4 bits (555), Expect = 4.2e-53
Identity = 101/151 (66.89%), Postives = 122/151 (80.79%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQIS IS+Q+QLKCSG KFYE + NK  S+ QMFP+ L SY+IVEGNGF +G++ HWKY
Sbjct: 1   MAQISNISKQLQLKCSGEKFYEFFRNKMDSITQMFPKKLLSYKIVEGNGFAHGTVVHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
           DFG P EAK +L MD+ N +ITLEF+EGDLF+DFE+  VK EVSDGG +G+SSVKW VEF
Sbjct: 61  DFGCPLEAKQKLHMDDTNIAITLEFIEGDLFKDFEMIAVKGEVSDGGSNGISSVKWSVEF 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYICNN 152
           VK NE V PPHNYLQF L+  K ++AY+CNN
Sbjct: 121 VKANEGVAPPHNYLQFALESTKGIEAYLCNN 151

[Close]

BLAST of MS027627.1 vs. NCBI nr
Match: XP_038887027.1 (MLP-like protein 31 [Benincasa hispida])

HSP 1 Score: 215.3 bits (547), Expect = 3.6e-52
Identity = 94/148 (63.51%), Postives = 120/148 (81.08%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQI  ISEQV+L C G KFY+ ++NK      MFP+ L+SY+ VEGNGFT+GS++HWKY
Sbjct: 1   MAQIFNISEQVKLNCCGEKFYDFFVNKMDYYVHMFPQRLKSYKFVEGNGFTHGSVTHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
           DFG P E K RL++DEPNK++  E LEGDL +DFE+F+VKV+V+DGG++ +SSVKW VEF
Sbjct: 61  DFGIPAEVKTRLLVDEPNKAVIFECLEGDLLKDFEMFQVKVQVTDGGKNAVSSVKWSVEF 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 149
           +K NEDV PPHNYLQFG+ +CK LDAY+
Sbjct: 121 MKANEDVAPPHNYLQFGVDVCKGLDAYL 148

[Close]

BLAST of MS027627.1 vs. NCBI nr
Match: XP_011651403.1 (major latex protein 15 [Cucumis sativus])

HSP 1 Score: 214.5 bits (545), Expect = 6.1e-52
Identity = 93/148 (62.84%), Postives = 120/148 (81.08%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQI  ISE+V+L C G KFY+ ++NK      +FP+NL+SY+ VEGN FT+GS++HWKY
Sbjct: 1   MAQICNISEEVKLNCCGDKFYDFFVNKMDCYTHLFPQNLQSYKFVEGNRFTHGSVTHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
           DFG P E K RL++DEPNK++  E +EGDL +DFE+F+VKVEV DGG++G+SSVKW V F
Sbjct: 61  DFGIPAEVKTRLLVDEPNKAVIFECVEGDLLKDFEMFQVKVEVRDGGKNGVSSVKWSVGF 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 149
           +K NEDV PPHNYLQFG+K+CK LDAY+
Sbjct: 121 MKANEDVAPPHNYLQFGVKVCKGLDAYL 148

[Close]

BLAST of MS027627.1 vs. ExPASy Swiss-Prot
Match: P85524 (Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-16
Identity = 44/141 (31.21%), Postives = 77/141 (54.61%), Query Frame = 0


Query: 9   EQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY--DFGSPT 68
           +QV++   G+ FYE +  +   + +M P N++  +++EGN  T GS+  +KY  D    T
Sbjct: 9   KQVEILSDGIVFYEIFRYRLYLISEMSPVNIQGVDLLEGNWGTVGSVIFFKYTIDGKEKT 68

Query: 69  EAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTNED 128
              +   +DE  KS+T + +EGDL   ++ F + V+V   G H  +SV W   + K  ED
Sbjct: 69  AKDIVEAIDEETKSVTFKIVEGDLMELYKTFIIIVQVDTKGEH--NSVTWTFHYEKLKED 128

Query: 129 VPPPHNYLQFGLKLCKALDAY 148
           V  P+  + F +++ K ++ Y
Sbjct: 129 VEEPNTLMNFCIEITKDIETY 147

[Close]

BLAST of MS027627.1 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 9.6e-16
Identity = 45/144 (31.25%), Postives = 78/144 (54.17%), Query Frame = 0


Query: 7   ISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY-DFGSP 66
           +  +V++K S  KF+  +  K   + +  P N++S ++ EG+  T GSI  W Y   G  
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  TEAKLRLVMDEPNKS-ITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTN 126
             AK R+   +P K+ IT   +EGDL ++++ F + ++V+       S V W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVPPPHNYLQFGLKLCKALDAYI 149
           E+V  P   LQF +++ K +D ++
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312

[Close]

BLAST of MS027627.1 vs. ExPASy Swiss-Prot
Match: Q941R6 (MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 5.8e-13
Identity = 41/146 (28.08%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 7   ISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY-DFGSP 66
           +   +++K S  KF+  +  +   + +  P  ++  E+ EG+    GSI  W Y   G  
Sbjct: 24  LETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYVHDGEA 83

Query: 67  TEAKLRLVMDEPNKS-ITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTN 126
             AK R+   EP K+ IT   +EGDL ++++ F + ++V+       S V W VE+ K +
Sbjct: 84  KVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYEKID 143

Query: 127 EDVPPPHNYLQFGLKLCKALDAYICN 151
           + V  P  +L F +++ K +D ++ N
Sbjct: 144 DKVAHPETFLDFCVEVSKEIDEHLLN 169

[Close]

BLAST of MS027627.1 vs. ExPASy Swiss-Prot
Match: Q06394 (Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 7.6e-13
Identity = 38/139 (27.34%), Postives = 67/139 (48.20%), Query Frame = 0


Query: 12  QLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKYDF-GSPTEAKL 71
           ++ C+  K+Y+ Y      L  + P    S + VEG+G T+G +  W Y   G P   K 
Sbjct: 19  EVNCNADKYYKLY-KHHEDLPSVIPHIYTSVKAVEGHGTTSGCVKEWGYILEGKPLSCKE 78

Query: 72  RLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTNEDVPPP 131
           +   ++  ++I    + GDL  D++ F   + V+         VKW +++ K NED P P
Sbjct: 79  KTTYNDETRTIHHMVVAGDLMNDYKKFDATLVVNPKSNGHGCIVKWTIDYEKMNEDSPVP 138

Query: 132 HNYLQFGLKLCKALDAYIC 150
             YL    ++ + L +++C
Sbjct: 139 FGYLACYQQITEDLSSHLC 156

[Close]

BLAST of MS027627.1 vs. ExPASy Swiss-Prot
Match: P19825 (Major latex protein 15 OS=Papaver somniferum OX=3469 GN=MLP15 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.2e-12
Identity = 38/139 (27.34%), Postives = 67/139 (48.20%), Query Frame = 0


Query: 12  QLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKYDF-GSPTEAKL 71
           ++ C+  K+Y+ +      L    P    S + VEG+G T+G +  W Y   G P   K 
Sbjct: 19  EVNCNADKYYQIF-KHHEDLPSAIPHIYTSVKAVEGHGTTSGCVKEWCYILEGKPLTVKE 78

Query: 72  RLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTNEDVPPP 131
           +   ++  ++I    +EG +  D++ F   + V        S V W V++ K NED P P
Sbjct: 79  KTTYNDETRTINHNGIEGGMMNDYKKFVATLVVKPKANGQGSIVTWIVDYEKINEDSPVP 138

Query: 132 HNYLQFGLKLCKALDAYIC 150
            +YL F  +  + L++++C
Sbjct: 139 FDYLAFFQQNIEDLNSHLC 156

[Close]

BLAST of MS027627.1 vs. ExPASy TrEMBL
Match: A0A6J1CQE9 (LOW QUALITY PROTEIN: major latex protein 149-like OS=Momordica charantia OX=3673 GN=LOC111013217 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.6e-77
Identity = 142/148 (95.95%), Postives = 143/148 (96.62%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQISIISEQVQLKCSGLKFYETYI K GSLHQMFPENL+SYEIVEGNGFTNG ISHWKY
Sbjct: 1   MAQISIISEQVQLKCSGLKFYETYIKKIGSLHQMFPENLKSYEIVEGNGFTNGIISHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
           DFGS TEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGG HGMSSVKWCVEF
Sbjct: 61  DFGSLTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGSHGMSSVKWCVEF 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 149
           VKTNEDVPPPHNYLQFGLKLCKALDAYI
Sbjct: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 148

[Close]

BLAST of MS027627.1 vs. ExPASy TrEMBL
Match: A0A1S3C5B3 (major latex protein 15-like OS=Cucumis melo OX=3656 GN=LOC103496826 PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.6e-53
Identity = 95/148 (64.19%), Postives = 122/148 (82.43%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQI  ISE+V+L C G KFY+ ++NK  S   +FP+NL+SY+ VEGNGFT+GS++HWKY
Sbjct: 1   MAQICNISEEVKLNCCGDKFYDFFLNKMDSYIHLFPQNLQSYKFVEGNGFTHGSVTHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
           DFG P E K RL++DEPNK++  E +EGDL +DFE+F+VKVEV DGG++G+SSVKW V F
Sbjct: 61  DFGIPAEVKTRLLVDEPNKAVIFECVEGDLLKDFEMFQVKVEVRDGGKNGVSSVKWSVGF 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYI 149
           +K NEDV PPHNYLQFG+K+CK LDAY+
Sbjct: 121 MKANEDVAPPHNYLQFGVKVCKGLDAYL 148

[Close]

BLAST of MS027627.1 vs. ExPASy TrEMBL
Match: A0A6J1CNR9 (uncharacterized protein LOC111013301 OS=Momordica charantia OX=3673 GN=LOC111013301 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.5e-51
Identity = 100/154 (64.94%), Postives = 123/154 (79.87%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQI+ ISEQVQLK  G KFYE   NK     +MFP N+ESY+  EGN FT+GSISHWKY
Sbjct: 1   MAQIAKISEQVQLKSCGHKFYEFLKNKMDYFPRMFPGNIESYKFAEGNSFTHGSISHWKY 60

Query: 61  D--FGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDG-GRHGMSSVKWC 120
           D   GS  E K+RL++DEPNK+I  E LEGDLF+DFE+F+VK+EVSDG G +G+SSVKW 
Sbjct: 61  DIGLGSSIEVKMRLIVDEPNKTIIYECLEGDLFKDFEMFQVKIEVSDGEGNNGISSVKWS 120

Query: 121 VEFVKTNEDVPPPHNYLQFGLKLCKALDAYICNN 152
           +EFVK NE+VPPPH+YLQ G+K+CK++DA +CNN
Sbjct: 121 LEFVKANEEVPPPHDYLQLGVKVCKSVDALLCNN 154

[Close]

BLAST of MS027627.1 vs. ExPASy TrEMBL
Match: A0A6J1F9L7 (kirola-like OS=Cucurbita moschata OX=3662 GN=LOC111442063 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.5e-51
Identity = 98/151 (64.90%), Postives = 119/151 (78.81%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQIS IS Q+QLKC+G +FYE Y NK   L QMFP+ L  Y+IVEGNGF +GS+ HWKY
Sbjct: 1   MAQISNISHQLQLKCTGEQFYEFYRNKMDRLTQMFPKKLLGYKIVEGNGFAHGSVIHWKY 60

Query: 61  DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEF 120
             G   EAK +L MD+ NK+ITLEF+EGDLF+++E+  VK EVSDGG +G+SSVKW VE+
Sbjct: 61  KLGCILEAKQKLHMDDKNKAITLEFIEGDLFKEYEMIAVKGEVSDGGSNGISSVKWSVEY 120

Query: 121 VKTNEDVPPPHNYLQFGLKLCKALDAYICNN 152
           VK NEDV PPHNYLQF L+L K +DAY+CNN
Sbjct: 121 VKANEDVDPPHNYLQFALELAKGVDAYLCNN 151

[Close]

BLAST of MS027627.1 vs. ExPASy TrEMBL
Match: A0A6J1CNR0 (kirola-like OS=Momordica charantia OX=3673 GN=LOC111013289 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.9e-51
Identity = 98/157 (62.42%), Postives = 120/157 (76.43%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MAQI+ ISEQVQLK  G KFY+ + NK      +FP+NL+ YE VEGNGFT+GS+ +W Y
Sbjct: 1   MAQIAKISEQVQLKSCGQKFYDHFANKMDRFAHLFPQNLKGYEFVEGNGFTHGSVMYWIY 60

Query: 61  -------DFGSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSS 120
                  + G P E K RL++DEPNKSIT E +EGDL +DFEVF+V+VEV+DGG  GMSS
Sbjct: 61  YWFVFGQNTGIPAEVKTRLMVDEPNKSITFECVEGDLLKDFEVFQVRVEVTDGGNSGMSS 120

Query: 121 VKWCVEFVKTNEDVPPPHNYLQFGLKLCKALDAYICN 151
           V+WC+EFVK NEDVP PHNYLQFG+K+CK LDAY+ N
Sbjct: 121 VRWCMEFVKKNEDVPSPHNYLQFGVKVCKGLDAYLSN 157

[Close]

BLAST of MS027627.1 vs. TAIR 10
Match: AT1G70850.1 (MLP-like protein 34 )

HSP 1 Score: 84.7 bits (208), Expect = 6.8e-17
Identity = 45/144 (31.25%), Postives = 78/144 (54.17%), Query Frame = 0


Query: 7   ISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY-DFGSP 66
           +  +V++K S  KF+  +  K   + +  P N++S ++ EG+  T GSI  W Y   G  
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  TEAKLRLVMDEPNKS-ITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTN 126
             AK R+   +P K+ IT   +EGDL ++++ F + ++V+       S V W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVPPPHNYLQFGLKLCKALDAYI 149
           E+V  P   LQF +++ K +D ++
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312

[Close]

BLAST of MS027627.1 vs. TAIR 10
Match: AT1G70850.3 (MLP-like protein 34 )

HSP 1 Score: 84.7 bits (208), Expect = 6.8e-17
Identity = 45/144 (31.25%), Postives = 78/144 (54.17%), Query Frame = 0


Query: 7   ISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY-DFGSP 66
           +  +V++K S  KF+  +  K   + +  P N++S ++ EG+  T GSI  W Y   G  
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  TEAKLRLVMDEPNKS-ITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTN 126
             AK R+   +P K+ IT   +EGDL ++++ F + ++V+       S V W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVPPPHNYLQFGLKLCKALDAYI 149
           E+V  P   LQF +++ K +D ++
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312

[Close]

BLAST of MS027627.1 vs. TAIR 10
Match: AT5G28010.1 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 7.5e-16
Identity = 43/141 (30.50%), Postives = 75/141 (53.19%), Query Frame = 0


Query: 10  QVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY-DFGSPTEA 69
           +V++K     FY  Y  +   + +  P N++S ++ +G   T GSI +W Y   G    A
Sbjct: 22  EVEIKAPAAIFYHIYAGRPHHVAKATPRNVQSCDLHDGEWGTVGSIVYWNYVHEGQAKVA 81

Query: 70  KLRLVMDEPNKS-ITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTNEDV 129
           K R+ + EP K  I    +EGD+  +++ F + ++V+       S VKW +E+ K +E+V
Sbjct: 82  KERIELVEPEKKLIKFRVIEGDVMAEYKSFLITIQVTPKEGGTGSVVKWHIEYEKIDENV 141

Query: 130 PPPHNYLQFGLKLCKALDAYI 149
           P P N L F  ++ K +D ++
Sbjct: 142 PHPENLLPFFAEMTKEIDEHL 162

[Close]

BLAST of MS027627.1 vs. TAIR 10
Match: AT1G70840.1 (MLP-like protein 31 )

HSP 1 Score: 75.5 bits (184), Expect = 4.1e-14
Identity = 41/146 (28.08%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 7   ISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY-DFGSP 66
           +   +++K S  KF+  +  +   + +  P  ++  E+ EG+    GSI  W Y   G  
Sbjct: 24  LETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYVHDGEA 83

Query: 67  TEAKLRLVMDEPNKS-ITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVEFVKTN 126
             AK R+   EP K+ IT   +EGDL ++++ F + ++V+       S V W VE+ K +
Sbjct: 84  KVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVEYEKID 143

Query: 127 EDVPPPHNYLQFGLKLCKALDAYICN 151
           + V  P  +L F +++ K +D ++ N
Sbjct: 144 DKVAHPETFLDFCVEVSKEIDEHLLN 169

[Close]

BLAST of MS027627.1 vs. TAIR 10
Match: AT1G23120.1 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 7.1e-14
Identity = 39/137 (28.47%), Postives = 73/137 (53.28%), Query Frame = 0


Query: 1   MAQISIISEQVQLKCSGLKFYETYINKFGSLHQMFPENLESYEIVEGNGFTNGSISHWKY 60
           MA +  +  ++Q+  +  +F++T+  K G+    F +  E+  +   +  +N SI  W +
Sbjct: 1   MAGVQTLELEIQVNMTAERFFKTFKKKEGN----FTDKTEAVYVHRDDPTSNSSIQIWNF 60

Query: 61  DF-GSPTEAKLRLVMDEPNKSITLEFLEGDLFRDFEVFKVKVEVSDGGRHGMSSVKWCVE 120
              G   + K ++ +DE NKS++   LEGD+ + ++ +K+ ++V     H +   KW  E
Sbjct: 61  IVDGKMEQIKEKIDVDEENKSVSFLALEGDVLKQYKSYKITLDVVPKD-HKVCIAKWTWE 120

Query: 121 FVKTNEDVPPPHNYLQF 137
           + K N+DVPPP  Y  F
Sbjct: 121 YEKLNDDVPPPTRYTAF 132

The following BLAST results are available for this feature:

BLAST of MS027627.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143318.1	3.2e-77	95.95	LOW QUALITY PROTEIN: major latex protein 149-like [Momordica charantia]	[more]
XP_008457056.1	3.2e-53	64.19	PREDICTED: major latex protein 15-like [Cucumis melo]	[more]
KAG6605298.1	4.2e-53	66.89	hypothetical protein SDJN03_02615, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038887027.1	3.6e-52	63.51	MLP-like protein 31 [Benincasa hispida]	[more]
XP_011651403.1	6.1e-52	62.84	major latex protein 15 [Cucumis sativus]	[more]

BLAST of MS027627.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P85524	1.1e-16	31.21	Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
Q9SSK7	9.6e-16	31.25	MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1	[more]
Q941R6	5.8e-13	28.08	MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2	[more]
Q06394	7.6e-13	27.34	Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1	[more]
P19825	2.2e-12	27.34	Major latex protein 15 OS=Papaver somniferum OX=3469 GN=MLP15 PE=2 SV=2	[more]

BLAST of MS027627.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQE9	1.6e-77	95.95	LOW QUALITY PROTEIN: major latex protein 149-like OS=Momordica charantia OX=3673...	[more]
A0A1S3C5B3	1.6e-53	64.19	major latex protein 15-like OS=Cucumis melo OX=3656 GN=LOC103496826 PE=3 SV=1	[more]
A0A6J1CNR9	1.5e-51	64.94	uncharacterized protein LOC111013301 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1F9L7	1.5e-51	64.90	kirola-like OS=Cucurbita moschata OX=3662 GN=LOC111442063 PE=3 SV=1	[more]
A0A6J1CNR0	1.9e-51	62.42	kirola-like OS=Momordica charantia OX=3673 GN=LOC111013289 PE=3 SV=1	[more]

BLAST of MS027627.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70850.1	6.8e-17	31.25	MLP-like protein 34	[more]
AT1G70850.3	6.8e-17	31.25	MLP-like protein 34	[more]
AT5G28010.1	7.5e-16	30.50	Polyketide cyclase/dehydrase and lipid transport superfamily protein	[more]
AT1G70840.1	4.1e-14	28.08	MLP-like protein 31	[more]
AT1G23120.1	7.1e-14	28.47	Polyketide cyclase/dehydrase and lipid transport superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I/Major latex protein	SMART	SM01037	Bet_v_1_2	coord: 2..151 e-value: 1.7E-17 score: 74.1
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 7..150 e-value: 1.1E-26 score: 93.6
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 1..151 e-value: 8.3E-34 score: 118.8
None	No IPR available	PANTHER	PTHR31907:SF52	MAJOR LATEX PROTEIN	coord: 1..147
None	No IPR available	PANTHER	PTHR31907	MLP-LIKE PROTEIN 423	coord: 1..147
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 2..150

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS027627	MS027627	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS027627.1-cds	MS027627.1-cds-scaffold20:1859921..1860107	CDS
MS027627.1-cds	MS027627.1-cds-scaffold20:1860274..1860542	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS027627.1	MS027627.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response