MS025854.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS025854.1 vs. NCBI nr
Match: XP_022143967.1 (acetylajmalan esterase-like, partial [Momordica charantia])

HSP 1 Score: 653.3 bits (1684), Expect = 1.3e-183
Identity = 316/340 (92.94%), Postives = 324/340 (95.29%), Query Frame = 0

Query: 18  SLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPVTPFSHLPYGETFFKKATGRC 77
           SLLLPSPSNAHLLKAC+FDAIYQLGDSISDTGNLIRENPVTPFSHLPYGETFFK ATGRC
Sbjct: 11  SLLLPSPSNAHLLKACKFDAIYQLGDSISDTGNLIRENPVTPFSHLPYGETFFKNATGRC 70

Query: 78  SNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGSTALSSQILAQKMILSPATNS 137
           SNGLLMIDQFALAAGLPLINPYLNK+ASF +GVNFAVAGSTALSSQ+LAQKMILSPATNS
Sbjct: 71  SNGLLMIDQFALAAGLPLINPYLNKDASFTHGVNFAVAGSTALSSQVLAQKMILSPATNS 130

Query: 138 SLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALFQGKTIEEVKNIVP 197
           SLTKQLDWMSTHF+TICS HK     LNDALFLVGEIGGNDYNYALFQGKTIEEVKN+VP
Sbjct: 131 SLTKQLDWMSTHFDTICSHHKGL--YLNDALFLVGEIGGNDYNYALFQGKTIEEVKNMVP 190

Query: 198 EVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAYDELHCLKDLNNLA 257
           EVVQ IKDA+ KVISYGA RVIVPGNFPIGCLPIYLTGFQTK STAYDELHCLKDLNNLA
Sbjct: 191 EVVQTIKDAIIKVISYGAVRVIVPGNFPIGCLPIYLTGFQTKISTAYDELHCLKDLNNLA 250

Query: 258 NYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDETSLQKSCCGIGGDY 317
           NYHNKHLKHAI+SL KDNPNVMIVY DYYNA LWVLR ASLLGFDETSLQKSCCGIGGDY
Sbjct: 251 NYHNKHLKHAIKSLRKDNPNVMIVYGDYYNAFLWVLRHASLLGFDETSLQKSCCGIGGDY 310

Query: 318 DFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 358
           DFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY
Sbjct: 311 DFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 348

BLAST of MS025854.1 vs. NCBI nr
Match: XP_022946882.1 (GDSL esterase/lipase At5g03980-like [Cucurbita moschata])

HSP 1 Score: 586.3 bits (1510), Expect = 1.9e-163
Identity = 278/374 (74.33%), Postives = 323/374 (86.36%), Query Frame = 0

Query: 1   MAVPTKS---ILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TKS   I+    I L S+L+   S AHLLK C FD IYQLGDSISDTGNLI +NP 
Sbjct: 1   MAPSTKSPSPIVLAVLIVLLSVLVCPSSTAHLLKTCMFDTIYQLGDSISDTGNLILQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYG++FF K+TGRCSNGLLMID FAL AGLPL+NPYLNK+AS R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGQSFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDASTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSS +L+QK ILSP TN+SL +QLDWM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSHLLSQKQILSPVTNTSLDQQLDWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI+EVK++VP+VVQ IK+AV KVISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQEVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN+LA YHN  +K AI+ L ++NP+ +I+Y DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNDLATYHNDQIKQAIQVLKRENPHSVIIYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDE SLQKSCCGIGGDY+F+L++MCG+ GV VCS+P+ER+SWDGIHLTQKAYKFMAY
Sbjct: 301 ILGFDEASLQKSCCGIGGDYNFSLVKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH IFP+L CIV
Sbjct: 361 WLIHDIFPQLHCIV 374

BLAST of MS025854.1 vs. NCBI nr
Match: XP_022998963.1 (acetylajmalan esterase-like [Cucurbita maxima] >XP_022998972.1 acetylajmalan esterase-like [Cucurbita maxima])

HSP 1 Score: 585.1 bits (1507), Expect = 4.2e-163
Identity = 280/374 (74.87%), Postives = 319/374 (85.29%), Query Frame = 0

Query: 1   MAVPTK---SILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TK   SI+    + L S++    S AHLLK C FDAIYQLGDSISDTGNLIR+NP 
Sbjct: 1   MAPSTKSPSSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYGET F K+TGRCSNGLLMID FAL AGLPL+NPYLNK+A  R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSSQ+L+QK ILSP TNSSL +QL WM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI++VK++VP+VVQ IK+AV K ISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN LA YHN  +K AI  L ++NP+ MIVY DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDETSLQKSCCGIGGDY+F+LM+MCG+ GV VCS+P+ER+SWDGIHLTQKAYKFMAY
Sbjct: 301 ILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH I P+L CIV
Sbjct: 361 WLIHDILPQLHCIV 374

BLAST of MS025854.1 vs. NCBI nr
Match: XP_022998964.1 (acetylajmalan esterase-like [Cucurbita maxima])

HSP 1 Score: 584.7 bits (1506), Expect = 5.5e-163
Identity = 280/374 (74.87%), Postives = 319/374 (85.29%), Query Frame = 0

Query: 1   MAVPTK---SILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TK   SI+    + L S++    S AHLLK C FDAIYQLGDSISDTGNLIR+NP 
Sbjct: 1   MAPSTKSPSSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYGET F K+TGRCSNGLLMID FAL AGLPL+NPYLNK+A  R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSSQ+L+QK ILSP TNSSL +QL WM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI++VK++VP+VVQ IK+AV KVISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN LA YHN  +K AI  L ++NP+ MIVY DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDETSLQKSCCGIGGDY+F+LM+MCG+ GV VC +P+ER+SWDGIHLTQKAYKFMAY
Sbjct: 301 ILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCXNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH I P+L CIV
Sbjct: 361 WLIHDILPQLHCIV 374

BLAST of MS025854.1 vs. NCBI nr
Match: XP_022998965.1 (acetylajmalan esterase-like [Cucurbita maxima] >XP_022998971.1 acetylajmalan esterase-like [Cucurbita maxima])

HSP 1 Score: 583.2 bits (1502), Expect = 1.6e-162
Identity = 279/374 (74.60%), Postives = 318/374 (85.03%), Query Frame = 0

Query: 1   MAVPTK---SILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TK   SI+    + L S++    S AHLLK C FDAIYQLGDSISDTGNLIR+NP 
Sbjct: 1   MAPSTKSPSSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYGET F K+TGRCSNGLLMID FAL AGLPL+NPYLNK+A  R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSSQ+L+QK ILSP TNSSL +QL WM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI++VK++VP+VVQ IK+AV K ISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN LA YHN  +K AI  L ++NP+ MIVY DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDETSLQKSCCGIGGDY+F+LM+MCG+ GV VCS+P+ER+SWDGIHLTQ AYKFMAY
Sbjct: 301 ILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQNAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH I P+L CIV
Sbjct: 361 WLIHDILPQLHCIV 374

BLAST of MS025854.1 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 7.2e-89
Identity = 170/357 (47.62%), Postives = 227/357 (63.59%), Query Frame = 0

Query: 16  LFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIR---ENPVTPFSHLPYGETFFKK 75
           +FSLL+ +     L+  C FD+IYQLGDS SDTGNLIR   + P    +H PYGETF   
Sbjct: 10  VFSLLVFAGITNGLI--CPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGT 69

Query: 76  ATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGSTALSSQILAQKMILS 135
            TGRCS+G L+ID  A A  LPL+NPYL +  SFR+GVNFAVAG+TAL    LA + +  
Sbjct: 70  PTGRCSDGRLIIDFIATALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQV 129

Query: 136 PATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALFQGKTIEEV 195
              +S L+ QL+W  T+  +ICS  K+C+ KL +ALF++G IG ND NYA F  +TIEE+
Sbjct: 130 SDIHSHLSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEI 189

Query: 196 KNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAYDELHCLKD 255
           +  VP + +A+ +A  ++I  G +RVIVPG FPIGC+   L           D+L CL  
Sbjct: 190 RAYVPFITEAVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSS 249

Query: 256 LNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDETSLQKSCCG 315
           LNNL+ Y N   + A+ SL  + P  +I+YADYYNA  ++ R    LG + TSL K CCG
Sbjct: 250 LNNLSIYFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCG 309

Query: 316 IGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAYWLIHHIFPKLRC 370
           IGG Y+++  + CG  GVPVC +P + I WDG H TQ AY+ +A ++I  I   L+C
Sbjct: 310 IGGPYNYDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKC 363

BLAST of MS025854.1 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 1.1e-76
Identity = 154/351 (43.87%), Postives = 217/351 (61.82%), Query Frame = 0

Query: 16  LFSLLLPSPSNAHLLKACR-FDAIYQLGDSISDTGNLI---RENPVTPFSHLPYGETFFK 75
           + S LL +  N+     CR F +I   GDSI+DTGNL+     N +   +  PYGETFF 
Sbjct: 16  ILSTLLVTSVNSQ--TQCRNFKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFH 75

Query: 76  KATGRCSNGLLMIDQFALAAGLPLINPYLN-KEASFRYGVNFAVAGSTALSSQILAQKMI 135
             TGR S+G L+ID  A   G PL+ P+   + A+F+ GVNFAVAG+TAL    L ++ I
Sbjct: 76  HPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGI 135

Query: 136 LSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALFQGKTIE 195
            S  TN SL+ QL   +     +C    DC + + +AL L+GEIGGNDYN+ALFQ K ++
Sbjct: 136 HSTITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVK 195

Query: 196 EVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAYDEL-HC 255
           EV+ +VP V+  I  A+T+++  G    +VPGNFPIG    YLT ++T N   YD L  C
Sbjct: 196 EVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGC 255

Query: 256 LKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDETSLQKS 315
           LK LN+ + Y+NK L+  +  L K  P+V I+YADYYNALL + +  +  GF    L  +
Sbjct: 256 LKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PA 315

Query: 316 CCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAYWLI 361
           CCG+GG Y+FN  + CG  GV  C DP++ +++DGIH+T+ AY+ ++  L+
Sbjct: 316 CCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of MS025854.1 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 9.1e-76
Identity = 152/356 (42.70%), Postives = 210/356 (58.99%), Query Frame = 0

Query: 8   ILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLI-----RENPVTPFSH 67
           + +T F+ + S   P P+         F +I   GDSI+DTGNL+      + PVT F  
Sbjct: 11  LFSTLFVTIVSSETPCPN---------FKSIISFGDSIADTGNLVGLSDRNQLPVTAFP- 70

Query: 68  LPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYL-NKEASFRYGVNFAVAGSTALS 127
            PYGETFF   TGR  +G +++D  A   GLP + PY  +K  +F  GVNFAVAG+TAL 
Sbjct: 71  -PYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALK 130

Query: 128 SQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNY 187
           S  L +K  + P TN SL  QL         +C    DC + + +AL L+GEIGGNDYN+
Sbjct: 131 SSFL-KKRGIQPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGNDYNF 190

Query: 188 ALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNS 247
             F  K ++EV+ +VP V+ +I   +T++I  G    +VPG FPIGC  +YLT ++T N 
Sbjct: 191 PFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNK 250

Query: 248 TAYD-ELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLG 307
             YD    CLK LN    YH++ LK  +  L K  P+V I+YADYYN+LL + +  +  G
Sbjct: 251 DEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFG 310

Query: 308 FDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMA 357
           F E     +CCGIGG Y+FN  + CG  GV  C DP++ + WDG+H+T+ AYK++A
Sbjct: 311 FMERPF-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIA 353

BLAST of MS025854.1 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.2e-75
Identity = 157/365 (43.01%), Postives = 208/365 (56.99%), Query Frame = 0

Query: 5   TKSILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPVTPFSHLP 64
           TK++    FI   SL+       H    C  ++IYQ GDSISDTGNLIR  P        
Sbjct: 4   TKALSLLVFILFVSLV-------HSSDQCPINSIYQFGDSISDTGNLIRNGP-------- 63

Query: 65  YGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGSTALSSQI 124
                                     A  P   P   +E      VNF V+GSTAL+S  
Sbjct: 64  --------------------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSF 123

Query: 125 LAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALF 184
            +++ +  PATN+ L+ QL W   H  + C  H   ++ L  +LF+VGEIGGNDYNY  F
Sbjct: 124 FSERNLHVPATNTPLSMQLAWFKGHLRSTC--HGSSSDCLKHSLFMVGEIGGNDYNYGFF 183

Query: 185 QGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAY 244
           QGK +EE+++ +P VV AI  A  +VI  GA  V+VPGNFP+GC PIYLT F  K++  Y
Sbjct: 184 QGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 243

Query: 245 DELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDET 304
           D+  CL  LN  A  HN  L+ AI SL K+ P+V IVY DYYNA  +VLR      FD++
Sbjct: 244 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSER---FDKS 303

Query: 305 SLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAYWLIHHIF 364
              KSCCG GG Y+++  +  G  GVPVC +P++ ISWDG+HLTQKAY+FM+ +L + I 
Sbjct: 304 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 365 PKLRC 370
            +++C
Sbjct: 364 SQIKC 320

BLAST of MS025854.1 vs. ExPASy Swiss-Prot
Match: Q9SHP6 (GDSL esterase/lipase At1g28610 OS=Arabidopsis thaliana OX=3702 GN=At1g28610 PE=2 SV=2)

HSP 1 Score: 283.1 bits (723), Expect = 4.5e-75
Identity = 150/336 (44.64%), Postives = 207/336 (61.61%), Query Frame = 0

Query: 33  CR-FDAIYQLGDSISDTGNLI-----RENPVTPFSHLPYGETFFKKATGRCSNGLLMIDQ 92
           CR  ++I   GDSI+DTGNL+        PVT F  LPYGETFF   TGR  NG ++ID 
Sbjct: 26  CRNLESIISFGDSITDTGNLVGLSDRNHLPVTAF--LPYGETFFHHPTGRSCNGRIIIDF 85

Query: 93  FALAAGLPLINP-YLNKEASFRYGVNFAVAGSTALSSQILAQKMILSPATNSSLTKQLDW 152
            A   GLP + P Y +K  +F  GVNFAVAG+TAL + IL ++ I  P +N SL  QL  
Sbjct: 86  IAEFLGLPHVPPFYGSKNGNFEKGVNFAVAGATALETSILEKRGIYYPHSNISLGIQLKT 145

Query: 153 MSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALFQGKTIEEVKNIVPEVVQAIKD 212
                  +C    DC + + +A  ++GEIGGND+N+A F  KT  EVK +VP V+  I  
Sbjct: 146 FKESLPNLCGSPTDCRDMIGNAFIIMGEIGGNDFNFAFFVNKT-SEVKELVPLVITKISS 205

Query: 213 AVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAYDEL-HCLKDLNNLANYHNKHL 272
           A+ +++  G    +VPGNFP+GC   YLT +QT N   YD L  CL  LN+ + Y+N+ L
Sbjct: 206 AIVELVDMGGRTFLVPGNFPLGCSATYLTLYQTSNKEEYDPLTGCLTWLNDFSEYYNEKL 265

Query: 273 KHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDETSLQKSCCGIGGDYDFNLMQM 332
           +  +  L K  P+V I+Y DY+NALL + +  S  GF +  L  +CCG+GG Y+F L + 
Sbjct: 266 QAELNRLSKLYPHVNIIYGDYFNALLRLYQEPSKFGFMDRPL-PACCGLGGPYNFTLSKK 325

Query: 333 CGLPGVPVCSDPNERISWDGIHLTQKAYKFMAYWLI 361
           CG  GV  CSDP++ ++WDG+H+T+ AYK++A  L+
Sbjct: 326 CGSVGVKYCSDPSKYVNWDGVHMTEAAYKWIADGLL 357

BLAST of MS025854.1 vs. ExPASy TrEMBL
Match: A0A6J1CSC4 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111013753 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.1e-184
Identity = 316/340 (92.94%), Postives = 324/340 (95.29%), Query Frame = 0

Query: 18  SLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPVTPFSHLPYGETFFKKATGRC 77
           SLLLPSPSNAHLLKAC+FDAIYQLGDSISDTGNLIRENPVTPFSHLPYGETFFK ATGRC
Sbjct: 11  SLLLPSPSNAHLLKACKFDAIYQLGDSISDTGNLIRENPVTPFSHLPYGETFFKNATGRC 70

Query: 78  SNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGSTALSSQILAQKMILSPATNS 137
           SNGLLMIDQFALAAGLPLINPYLNK+ASF +GVNFAVAGSTALSSQ+LAQKMILSPATNS
Sbjct: 71  SNGLLMIDQFALAAGLPLINPYLNKDASFTHGVNFAVAGSTALSSQVLAQKMILSPATNS 130

Query: 138 SLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALFQGKTIEEVKNIVP 197
           SLTKQLDWMSTHF+TICS HK     LNDALFLVGEIGGNDYNYALFQGKTIEEVKN+VP
Sbjct: 131 SLTKQLDWMSTHFDTICSHHKGL--YLNDALFLVGEIGGNDYNYALFQGKTIEEVKNMVP 190

Query: 198 EVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAYDELHCLKDLNNLA 257
           EVVQ IKDA+ KVISYGA RVIVPGNFPIGCLPIYLTGFQTK STAYDELHCLKDLNNLA
Sbjct: 191 EVVQTIKDAIIKVISYGAVRVIVPGNFPIGCLPIYLTGFQTKISTAYDELHCLKDLNNLA 250

Query: 258 NYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDETSLQKSCCGIGGDY 317
           NYHNKHLKHAI+SL KDNPNVMIVY DYYNA LWVLR ASLLGFDETSLQKSCCGIGGDY
Sbjct: 251 NYHNKHLKHAIKSLRKDNPNVMIVYGDYYNAFLWVLRHASLLGFDETSLQKSCCGIGGDY 310

Query: 318 DFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 358
           DFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY
Sbjct: 311 DFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 348

BLAST of MS025854.1 vs. ExPASy TrEMBL
Match: A0A6J1G4V8 (GDSL esterase/lipase At5g03980-like OS=Cucurbita moschata OX=3662 GN=LOC111450822 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 9.2e-164
Identity = 278/374 (74.33%), Postives = 323/374 (86.36%), Query Frame = 0

Query: 1   MAVPTKS---ILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TKS   I+    I L S+L+   S AHLLK C FD IYQLGDSISDTGNLI +NP 
Sbjct: 1   MAPSTKSPSPIVLAVLIVLLSVLVCPSSTAHLLKTCMFDTIYQLGDSISDTGNLILQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYG++FF K+TGRCSNGLLMID FAL AGLPL+NPYLNK+AS R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGQSFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDASTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSS +L+QK ILSP TN+SL +QLDWM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSHLLSQKQILSPVTNTSLDQQLDWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI+EVK++VP+VVQ IK+AV KVISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQEVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN+LA YHN  +K AI+ L ++NP+ +I+Y DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNDLATYHNDQIKQAIQVLKRENPHSVIIYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDE SLQKSCCGIGGDY+F+L++MCG+ GV VCS+P+ER+SWDGIHLTQKAYKFMAY
Sbjct: 301 ILGFDEASLQKSCCGIGGDYNFSLVKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH IFP+L CIV
Sbjct: 361 WLIHDIFPQLHCIV 374

BLAST of MS025854.1 vs. ExPASy TrEMBL
Match: A0A6J1K9I7 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493500 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.1e-163
Identity = 280/374 (74.87%), Postives = 319/374 (85.29%), Query Frame = 0

Query: 1   MAVPTK---SILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TK   SI+    + L S++    S AHLLK C FDAIYQLGDSISDTGNLIR+NP 
Sbjct: 1   MAPSTKSPSSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYGET F K+TGRCSNGLLMID FAL AGLPL+NPYLNK+A  R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSSQ+L+QK ILSP TNSSL +QL WM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI++VK++VP+VVQ IK+AV K ISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN LA YHN  +K AI  L ++NP+ MIVY DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDETSLQKSCCGIGGDY+F+LM+MCG+ GV VCS+P+ER+SWDGIHLTQKAYKFMAY
Sbjct: 301 ILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH I P+L CIV
Sbjct: 361 WLIHDILPQLHCIV 374

BLAST of MS025854.1 vs. ExPASy TrEMBL
Match: A0A6J1KFS3 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493492 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.7e-163
Identity = 280/374 (74.87%), Postives = 319/374 (85.29%), Query Frame = 0

Query: 1   MAVPTK---SILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TK   SI+    + L S++    S AHLLK C FDAIYQLGDSISDTGNLIR+NP 
Sbjct: 1   MAPSTKSPSSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYGET F K+TGRCSNGLLMID FAL AGLPL+NPYLNK+A  R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSSQ+L+QK ILSP TNSSL +QL WM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI++VK++VP+VVQ IK+AV KVISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN LA YHN  +K AI  L ++NP+ MIVY DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDETSLQKSCCGIGGDY+F+LM+MCG+ GV VC +P+ER+SWDGIHLTQKAYKFMAY
Sbjct: 301 ILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCXNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH I P+L CIV
Sbjct: 361 WLIHDILPQLHCIV 374

BLAST of MS025854.1 vs. ExPASy TrEMBL
Match: A0A6J1KDZ5 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493493 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 7.8e-163
Identity = 279/374 (74.60%), Postives = 318/374 (85.03%), Query Frame = 0

Query: 1   MAVPTK---SILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPV 60
           MA  TK   SI+    + L S++    S AHLLK C FDAIYQLGDSISDTGNLIR+NP 
Sbjct: 1   MAPSTKSPSSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGS 120
           TPFSHLPYGET F K+TGRCSNGLLMID FAL AGLPL+NPYLNK+A  R+GVNFAVAGS
Sbjct: 61  TPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGN 180
           TALSSQ+L+QK ILSP TNSSL +QL WM +HFN+IC   +DC EKL +ALFLVGEIGGN
Sbjct: 121 TALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGN 180

Query: 181 DYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQ 240
           DYNYALFQGKTI++VK++VP+VVQ IK+AV K ISYGATRV+VPGNFPIGCLPIYLTGFQ
Sbjct: 181 DYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TKNSTAYDELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRAS 300
           T ++TAYDELHCLKDLN LA YHN  +K AI  L ++NP+ MIVY DYYNALLW+LRRA 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAF 300

Query: 301 LLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAY 360
           +LGFDETSLQKSCCGIGGDY+F+LM+MCG+ GV VCS+P+ER+SWDGIHLTQ AYKFMAY
Sbjct: 301 ILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQNAYKFMAY 360

Query: 361 WLIHHIFPKLRCIV 372
           WLIH I P+L CIV
Sbjct: 361 WLIHDILPQLHCIV 374

BLAST of MS025854.1 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 7.6e-78
Identity = 154/351 (43.87%), Postives = 217/351 (61.82%), Query Frame = 0

Query: 16  LFSLLLPSPSNAHLLKACR-FDAIYQLGDSISDTGNLI---RENPVTPFSHLPYGETFFK 75
           + S LL +  N+     CR F +I   GDSI+DTGNL+     N +   +  PYGETFF 
Sbjct: 16  ILSTLLVTSVNSQ--TQCRNFKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFH 75

Query: 76  KATGRCSNGLLMIDQFALAAGLPLINPYLN-KEASFRYGVNFAVAGSTALSSQILAQKMI 135
             TGR S+G L+ID  A   G PL+ P+   + A+F+ GVNFAVAG+TAL    L ++ I
Sbjct: 76  HPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGI 135

Query: 136 LSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALFQGKTIE 195
            S  TN SL+ QL   +     +C    DC + + +AL L+GEIGGNDYN+ALFQ K ++
Sbjct: 136 HSTITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVK 195

Query: 196 EVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAYDEL-HC 255
           EV+ +VP V+  I  A+T+++  G    +VPGNFPIG    YLT ++T N   YD L  C
Sbjct: 196 EVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGC 255

Query: 256 LKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDETSLQKS 315
           LK LN+ + Y+NK L+  +  L K  P+V I+YADYYNALL + +  +  GF    L  +
Sbjct: 256 LKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PA 315

Query: 316 CCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAYWLI 361
           CCG+GG Y+FN  + CG  GV  C DP++ +++DGIH+T+ AY+ ++  L+
Sbjct: 316 CCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of MS025854.1 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 285.4 bits (729), Expect = 6.5e-77
Identity = 152/356 (42.70%), Postives = 210/356 (58.99%), Query Frame = 0

Query: 8   ILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLI-----RENPVTPFSH 67
           + +T F+ + S   P P+         F +I   GDSI+DTGNL+      + PVT F  
Sbjct: 11  LFSTLFVTIVSSETPCPN---------FKSIISFGDSIADTGNLVGLSDRNQLPVTAFP- 70

Query: 68  LPYGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYL-NKEASFRYGVNFAVAGSTALS 127
            PYGETFF   TGR  +G +++D  A   GLP + PY  +K  +F  GVNFAVAG+TAL 
Sbjct: 71  -PYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALK 130

Query: 128 SQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNY 187
           S  L +K  + P TN SL  QL         +C    DC + + +AL L+GEIGGNDYN+
Sbjct: 131 SSFL-KKRGIQPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGNDYNF 190

Query: 188 ALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNS 247
             F  K ++EV+ +VP V+ +I   +T++I  G    +VPG FPIGC  +YLT ++T N 
Sbjct: 191 PFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNK 250

Query: 248 TAYD-ELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLG 307
             YD    CLK LN    YH++ LK  +  L K  P+V I+YADYYN+LL + +  +  G
Sbjct: 251 DEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFG 310

Query: 308 FDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMA 357
           F E     +CCGIGG Y+FN  + CG  GV  C DP++ + WDG+H+T+ AYK++A
Sbjct: 311 FMERPF-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIA 353

BLAST of MS025854.1 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 285.0 bits (728), Expect = 8.4e-77
Identity = 157/365 (43.01%), Postives = 208/365 (56.99%), Query Frame = 0

Query: 5   TKSILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPVTPFSHLP 64
           TK++    FI   SL+       H    C  ++IYQ GDSISDTGNLIR  P        
Sbjct: 4   TKALSLLVFILFVSLV-------HSSDQCPINSIYQFGDSISDTGNLIRNGP-------- 63

Query: 65  YGETFFKKATGRCSNGLLMIDQFALAAGLPLINPYLNKEASFRYGVNFAVAGSTALSSQI 124
                                     A  P   P   +E      VNF V+GSTAL+S  
Sbjct: 64  --------------------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSF 123

Query: 125 LAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALF 184
            +++ +  PATN+ L+ QL W   H  + C  H   ++ L  +LF+VGEIGGNDYNY  F
Sbjct: 124 FSERNLHVPATNTPLSMQLAWFKGHLRSTC--HGSSSDCLKHSLFMVGEIGGNDYNYGFF 183

Query: 185 QGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAY 244
           QGK +EE+++ +P VV AI  A  +VI  GA  V+VPGNFP+GC PIYLT F  K++  Y
Sbjct: 184 QGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 243

Query: 245 DELHCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDET 304
           D+  CL  LN  A  HN  L+ AI SL K+ P+V IVY DYYNA  +VLR      FD++
Sbjct: 244 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSER---FDKS 303

Query: 305 SLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFMAYWLIHHIF 364
              KSCCG GG Y+++  +  G  GVPVC +P++ ISWDG+HLTQKAY+FM+ +L + I 
Sbjct: 304 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 365 PKLRC 370
            +++C
Sbjct: 364 SQIKC 320

BLAST of MS025854.1 vs. TAIR 10
Match: AT1G28610.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.2e-76
Identity = 150/336 (44.64%), Postives = 207/336 (61.61%), Query Frame = 0

Query: 33  CR-FDAIYQLGDSISDTGNLI-----RENPVTPFSHLPYGETFFKKATGRCSNGLLMIDQ 92
           CR  ++I   GDSI+DTGNL+        PVT F  LPYGETFF   TGR  NG ++ID 
Sbjct: 26  CRNLESIISFGDSITDTGNLVGLSDRNHLPVTAF--LPYGETFFHHPTGRSCNGRIIIDF 85

Query: 93  FALAAGLPLINP-YLNKEASFRYGVNFAVAGSTALSSQILAQKMILSPATNSSLTKQLDW 152
            A   GLP + P Y +K  +F  GVNFAVAG+TAL + IL ++ I  P +N SL  QL  
Sbjct: 86  IAEFLGLPHVPPFYGSKNGNFEKGVNFAVAGATALETSILEKRGIYYPHSNISLGIQLKT 145

Query: 153 MSTHFNTICSRHKDCTEKLNDALFLVGEIGGNDYNYALFQGKTIEEVKNIVPEVVQAIKD 212
                  +C    DC + + +A  ++GEIGGND+N+A F  KT  EVK +VP V+  I  
Sbjct: 146 FKESLPNLCGSPTDCRDMIGNAFIIMGEIGGNDFNFAFFVNKT-SEVKELVPLVITKISS 205

Query: 213 AVTKVISYGATRVIVPGNFPIGCLPIYLTGFQTKNSTAYDEL-HCLKDLNNLANYHNKHL 272
           A+ +++  G    +VPGNFP+GC   YLT +QT N   YD L  CL  LN+ + Y+N+ L
Sbjct: 206 AIVELVDMGGRTFLVPGNFPLGCSATYLTLYQTSNKEEYDPLTGCLTWLNDFSEYYNEKL 265

Query: 273 KHAIRSLGKDNPNVMIVYADYYNALLWVLRRASLLGFDETSLQKSCCGIGGDYDFNLMQM 332
           +  +  L K  P+V I+Y DY+NALL + +  S  GF +  L  +CCG+GG Y+F L + 
Sbjct: 266 QAELNRLSKLYPHVNIIYGDYFNALLRLYQEPSKFGFMDRPL-PACCGLGGPYNFTLSKK 325

Query: 333 CGLPGVPVCSDPNERISWDGIHLTQKAYKFMAYWLI 361
           CG  GV  CSDP++ ++WDG+H+T+ AYK++A  L+
Sbjct: 326 CGSVGVKYCSDPSKYVNWDGVHMTEAAYKWIADGLL 357

BLAST of MS025854.1 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 4.2e-76
Identity = 153/366 (41.80%), Postives = 208/366 (56.83%), Query Frame = 0

Query: 1   MAVPTKSILATAFICLFSLLLPSPSNAHLLKACRFDAIYQLGDSISDTGNLIRENPVTPF 60
           MA P   IL    + +F       + +   K   F +I   GDSI+DTGNL+  +     
Sbjct: 1   MAYPGSPILMKLLVFIFLSTFVVTNVSSETKCREFKSIISFGDSIADTGNLLGLSDPKDL 60

Query: 61  SHL---PYGETFFKKATGRCSNGLLMIDQFALAAGLPLINP-YLNKEASFRYGVNFAVAG 120
            H+   PYGE FF   TGR SNG L+ID  A   GLPL+ P Y +  A+F  GVNFAV G
Sbjct: 61  PHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNFAVGG 120

Query: 121 STALSSQILAQKMILSPATNSSLTKQLDWMSTHFNTICSRHKDCTEKLNDALFLVGEIGG 180
           +TAL    L  + I  P TN SL  QL+       +IC    DC + + +AL L+GEIGG
Sbjct: 121 ATALERSFLEDRGIHFPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGG 180

Query: 181 NDYNYALFQGKTIEEVKNIVPEVVQAIKDAVTKVISYGATRVIVPGNFPIGCLPIYLTGF 240
           NDYNYA F  K IEE+K ++P V+  I  A+T++I  G    +VPG FP+GC  +YLT  
Sbjct: 181 NDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSH 240

Query: 241 QTKNSTAYDEL-HCLKDLNNLANYHNKHLKHAIRSLGKDNPNVMIVYADYYNALLWVLRR 300
           QT N   YD L  CLK LN     H + L+  +  L K  P+V I+YADYYNAL  + + 
Sbjct: 241 QTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQE 300

Query: 301 ASLLGFDETSLQKSCCGIGGDYDFNLMQMCGLPGVPVCSDPNERISWDGIHLTQKAYKFM 360
            +  GF    L  +CCG GG Y++ + + CG   V  C DP++ ++WDG+H+T+ AY+ M
Sbjct: 301 PAKFGFMNRPL-SACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLM 360

Query: 361 AYWLIH 362
           A  +++
Sbjct: 361 AEGILN 365