MS025055.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS025055.1 vs. NCBI nr
Match: XP_022141681.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 278.5 bits (711), Expect = 4.5e-71
Identity = 151/247 (61.13%), Postives = 165/247 (66.80%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDPI  KNIWDGYIPP S+S+ESIL+LTA L+WEQA EPLHWDIDY+KT   
Sbjct: 25  MAGRGGVSKDPITEKNIWDGYIPPESQSNESILRLTADLRWEQASEPLHWDIDYHKTNGI 84

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+L  + GKSIGVIGLVPCAIGGTHLREW+KG                     
Sbjct: 85  GPGMAFANELSVQVGKSIGVIGLVPCAIGGTHLREWIKGTAYYTKLIDRIKASEKHGGKV 144

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDASVEEESK YE  LTKFFTDLR DS +  LPIIL               
Sbjct: 145 QGFLWYQGESDASVEEESKSYETELTKFFTDLRTDSNNLELPIILVKIVTHDIFTSPIIN 204

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 188
                  A+E VT KL NVRMVDG +AVGN ++GLNED+GHLNVKSEVKLGKMLAHAFYS
Sbjct: 205 FKEDVWKAQETVTEKLSNVRMVDGSEAVGNFEEGLNEDKGHLNVKSEVKLGKMLAHAFYS 264

BLAST of MS025055.1 vs. NCBI nr
Match: KAG7016422.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265.4 bits (677), Expect = 3.9e-67
Identity = 142/244 (58.20%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDP   KN+WDGYIPP S+ ++SI + TA + WEQAREPLHWDID  KT   
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+LLAKAG SIG IGLVPCAIGG+HLREWVKG                     
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEE+K YE  L+KFFTDLR D  HP LPIIL               
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 185
                  A+EAVT KLPNVRMVDG  AVGN D+GLNEDRGHLNVKSEV LGKM AH++YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

BLAST of MS025055.1 vs. NCBI nr
Match: XP_022993914.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 265.0 bits (676), Expect = 5.2e-67
Identity = 142/244 (58.20%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDP   KN+WDGYIPP S+ ++SI + TA + WEQAREPLHWDID  KT   
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+LLAKAG SIG IGLVPCAIGG+HLREWVKG                     
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEE+K YE  L+KFFTDLR D  HP LPIIL               
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADVNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 185
                  A+EAVT KLPNVRMVDG  AVGN D+GLNEDRGHLNVKSEV LGKM AH++YS
Sbjct: 198 FKDEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

BLAST of MS025055.1 vs. NCBI nr
Match: XP_022939276.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 265.0 bits (676), Expect = 5.2e-67
Identity = 141/244 (57.79%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDP   KN+WDGYIPP S+ ++SI + TA + WEQAREPLHWDID  KT   
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+LLAKAG SIG IGLVPCAIGG+HLREWVKG                     
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTNRYTKLVERMKRSEEHGGKV 137

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEE+K YE  L+KFFTDLR D  HP LPIIL               
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 185
                  A+EAVT KLPN+RMVDG  AVGN D+GLNEDRGHLNVKSEV LGKM AH++YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNIRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

BLAST of MS025055.1 vs. NCBI nr
Match: KAG6578894.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 264.2 bits (674), Expect = 8.8e-67
Identity = 141/244 (57.79%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDP   KN+WDGYIPP S+ ++SI + TA + WEQAREPLHWDID  KT   
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+LLAKAG SIG IGLVPCAIGG+HLREWVKG                     
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEE+K YE  L+KFFTDLR D  HP LPIIL               
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 185
                  A+EAVT KLPNVRMVDG  AVGN D+GLNEDRGHLNV+SEV LGKM AH++YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVRSEVNLGKMFAHSYYS 257

BLAST of MS025055.1 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 81/235 (34.47%), Postives = 108/235 (45.96%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNK---- 60
           MAGRGGV KD    + +WD  +PP    + SIL+L+A L+WE+A EPLH DID  K    
Sbjct: 33  MAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVDIDTGKVCGV 92

Query: 61  ------TNKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N +  +      VIGLVPCA GGT ++EW +G                     
Sbjct: 93  GPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVKRTEESRKCGGEI 152

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPII-------------LAE 180
                  GESD     +++ Y  N+ +   +LR D   P+LPII             + E
Sbjct: 153 KAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAIASGGGYIDKVRE 212

Query: 181 EAVTHKLPNVRMVDGMKAVGNIDKG--LNEDRGHLNVKSEVKLGKMLAHAFYSNF 183
             +  KL NV  VD         KG  L  D  HL  +++V+LG  LA A+ SNF
Sbjct: 213 AQLGLKLSNVVCVDA--------KGLPLKSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of MS025055.1 vs. ExPASy TrEMBL
Match: A0A6J1CJZ1 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111011984 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.2e-71
Identity = 151/247 (61.13%), Postives = 165/247 (66.80%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDPI  KNIWDGYIPP S+S+ESIL+LTA L+WEQA EPLHWDIDY+KT   
Sbjct: 25  MAGRGGVSKDPITEKNIWDGYIPPESQSNESILRLTADLRWEQASEPLHWDIDYHKTNGI 84

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+L  + GKSIGVIGLVPCAIGGTHLREW+KG                     
Sbjct: 85  GPGMAFANELSVQVGKSIGVIGLVPCAIGGTHLREWIKGTAYYTKLIDRIKASEKHGGKV 144

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDASVEEESK YE  LTKFFTDLR DS +  LPIIL               
Sbjct: 145 QGFLWYQGESDASVEEESKSYETELTKFFTDLRTDSNNLELPIILVKIVTHDIFTSPIIN 204

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 188
                  A+E VT KL NVRMVDG +AVGN ++GLNED+GHLNVKSEVKLGKMLAHAFYS
Sbjct: 205 FKEDVWKAQETVTEKLSNVRMVDGSEAVGNFEEGLNEDKGHLNVKSEVKLGKMLAHAFYS 264

BLAST of MS025055.1 vs. ExPASy TrEMBL
Match: A0A6J1K1G7 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111489769 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.5e-67
Identity = 142/244 (58.20%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDP   KN+WDGYIPP S+ ++SI + TA + WEQAREPLHWDID  KT   
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+LLAKAG SIG IGLVPCAIGG+HLREWVKG                     
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKLVERMKRSEEHGGKV 137

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEE+K YE  L+KFFTDLR D  HP LPIIL               
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADVNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 185
                  A+EAVT KLPNVRMVDG  AVGN D+GLNEDRGHLNVKSEV LGKM AH++YS
Sbjct: 198 FKDEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

BLAST of MS025055.1 vs. ExPASy TrEMBL
Match: A0A6J1FFF9 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111445241 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.5e-67
Identity = 141/244 (57.79%), Postives = 157/244 (64.34%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVSKDP   KN+WDGYIPP S+ ++SI + TA + WEQAREPLHWDID  KT   
Sbjct: 18  MAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQAREPLHWDIDVVKTNGV 77

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+LLAKAG SIG IGLVPCAIGG+HLREWVKG                     
Sbjct: 78  GPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTNRYTKLVERMKRSEEHGGKV 137

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEE+K YE  L+KFFTDLR D  HP LPIIL               
Sbjct: 138 KGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILVKIVTHDFFISPDFE 197

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 185
                  A+EAVT KLPN+RMVDG  AVGN D+GLNEDRGHLNVKSEV LGKM AH++YS
Sbjct: 198 FKEEVWNAQEAVTQKLPNIRMVDGRVAVGNFDEGLNEDRGHLNVKSEVNLGKMFAHSYYS 257

BLAST of MS025055.1 vs. ExPASy TrEMBL
Match: A0A0A0K9J9 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G058180 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 4.1e-62
Identity = 136/248 (54.84%), Postives = 150/248 (60.48%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKT--- 60
           MAGRGGVS DP   K +WDGYIP    S++SI +L A + WEQA EPLHWDID  KT   
Sbjct: 26  MAGRGGVSLDPTTDKMVWDGYIPLECESNDSIFRLNADMVWEQAHEPLHWDIDVVKTNGI 85

Query: 61  -------NKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N+LLA  GK IG IGLVPCAIGG+HL+EWVKG                     
Sbjct: 86  GPGMAFANELLAIGGKRIGAIGLVPCAIGGSHLKEWVKGTNRYDNLVERIRASEKNGGTV 145

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEE+  YE  LTKFF DLR D+ HP LPIIL               
Sbjct: 146 QGILWYQGESDAAVEEEAMCYERELTKFFIDLRADTNHPELPIILVKLVTHDFFLSPNIS 205

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 189
                  A EAVTH+LPNV MVDG  AVGN D GLNED+GHLNVKSEVKLGKM AH+FYS
Sbjct: 206 FKEEVCNALEAVTHRLPNVTMVDGPMAVGNFDDGLNEDKGHLNVKSEVKLGKMFAHSFYS 265

BLAST of MS025055.1 vs. ExPASy TrEMBL
Match: A0A6J1CKF9 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111012113 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.6e-61
Identity = 131/248 (52.82%), Postives = 156/248 (62.90%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKTN-- 60
           MAGRGGV KDP   K +WDG +PP  + ++SIL+ +A+  WE+A EPLHWDID NKTN  
Sbjct: 39  MAGRGGVEKDPNTQKMVWDGIVPPKCQPNKSILRFSANSVWEEALEPLHWDIDVNKTNGI 98

Query: 61  --------KLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                   ++LAKAG   GVIGLVPCAIGGTHLREWVKG                     
Sbjct: 99  GPGMPFAHEILAKAGNKSGVIGLVPCAIGGTHLREWVKGTQNYTRLVNRIKASEAQGGKI 158

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPIIL--------------- 180
                  GESDA+VEEESK+YE NLTKF+TDLR D+ HP LPIIL               
Sbjct: 159 QGLLWYQGESDAAVEEESKFYESNLTKFYTDLRTDTNHPDLPIILVKIVTHDFFISPLIN 218

Query: 181 -------AEEAVTHKLPNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYS 189
                  A+E +T  L NVR+VDG +AVGN D G+N+D GHL+ KSEVKLGKMLA +FYS
Sbjct: 219 FLKDVWKAQEDITRDLVNVRIVDGKQAVGNFDTGMNQDGGHLSTKSEVKLGKMLADSFYS 278

BLAST of MS025055.1 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 133.7 bits (335), Expect = 1.7e-31
Identity = 87/236 (36.86%), Postives = 112/236 (47.46%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNKTN-- 60
           MAGRGGV  D      +WDG IPP  RS+ SIL+LT+ L+W++A+EPLH DID NKTN  
Sbjct: 40  MAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHVDIDINKTNGV 99

Query: 61  ----KLLAKAGKSIGVIGLVPCAIGGTHLREWVKG------------------------- 120
                   +     G +GLVPC+IGGT L +W KG                         
Sbjct: 100 GPGMPFANRVVNRFGQVGLVPCSIGGTKLSQWQKGEFLYEETVKRAKAAMASGGGGSYRA 159

Query: 121 -----GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPII---LAEEA-----VTHKL 180
                GESD     ++  Y+  L KFF+DLR D  HP LPII   LA  A        K 
Sbjct: 160 VLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPIIQVALATGAGPYLDAVRKA 219

Query: 181 PNVRMVDGMKAVGNIDKGLNEDRGHLNVKSEVKLGKMLAHAFYSNFGHNLTSLNLH 193
                ++ +  V      L  D  HL   S+V+LG M+A +F +       SL +H
Sbjct: 220 QLKTDLENVYCVDARGLPLEPDGLHLTTSSQVQLGHMIAESFLAIPNSARPSLYMH 275

BLAST of MS025055.1 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 114.8 bits (286), Expect = 8.0e-26
Identity = 81/235 (34.47%), Postives = 108/235 (45.96%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNK---- 60
           MAGRGGV KD    + +WD  +PP    + SIL+L+A L+WE+A EPLH DID  K    
Sbjct: 33  MAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVDIDTGKVCGV 92

Query: 61  ------TNKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N +  +      VIGLVPCA GGT ++EW +G                     
Sbjct: 93  GPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVKRTEESRKCGGEI 152

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPII-------------LAE 180
                  GESD     +++ Y  N+ +   +LR D   P+LPII             + E
Sbjct: 153 KAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAIASGGGYIDKVRE 212

Query: 181 EAVTHKLPNVRMVDGMKAVGNIDKG--LNEDRGHLNVKSEVKLGKMLAHAFYSNF 183
             +  KL NV  VD         KG  L  D  HL  +++V+LG  LA A+ SNF
Sbjct: 213 AQLGLKLSNVVCVDA--------KGLPLKSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of MS025055.1 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 114.8 bits (286), Expect = 8.0e-26
Identity = 81/235 (34.47%), Postives = 108/235 (45.96%), Query Frame = 0

Query: 1   MAGRGGVSKDPIIGKNIWDGYIPPASRSHESILQLTAHLKWEQAREPLHWDIDYNK---- 60
           MAGRGGV KD    + +WD  +PP    + SIL+L+A L+WE+A EPLH DID  K    
Sbjct: 33  MAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVDIDTGKVCGV 92

Query: 61  ------TNKLLAKAGKSIGVIGLVPCAIGGTHLREWVKG--------------------- 120
                  N +  +      VIGLVPCA GGT ++EW +G                     
Sbjct: 93  GPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVKRTEESRKCGGEI 152

Query: 121 -------GESDASVEEESKYYEGNLTKFFTDLRKDSYHPTLPII-------------LAE 180
                  GESD     +++ Y  N+ +   +LR D   P+LPII             + E
Sbjct: 153 KAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAIASGGGYIDKVRE 212

Query: 181 EAVTHKLPNVRMVDGMKAVGNIDKG--LNEDRGHLNVKSEVKLGKMLAHAFYSNF 183
             +  KL NV  VD         KG  L  D  HL  +++V+LG  LA A+ SNF
Sbjct: 213 AQLGLKLSNVVCVDA--------KGLPLKSDNLHLTTEAQVQLGLSLAQAYLSNF 259