MS023152.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS023152.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold78: 484873 .. 488588 (+)
Sequence length	1479
RNA-Seq Expression	MS023152.1
Synteny	MS023152.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGTGGAGACGTCAATTCTGGGTGGGCGGTTCCATAGCCAGAGAATTGACTTCAAGCGATGCATTCCGGCTTTCTTCTCCACCTACAGGACGCTCTTTACAGTGTTCTGGATCGCGGCGGTTGCCACGATTTTTCTCTGGCAGGGTACTATAGGTAATGGGTTCTTGTCGCTCCGGCGAGCCTCCGCCAGACCGTTGCCGCGGCTGAGGCCTGTTGCGTTCAATTTGACGGATTTTGGAGCAGTGGGCGACGGGGTGACATTGAATACCGAGGCCTTTGAGAGGGCTATTTTGGCTATTTCGAAGTTGAGGACCAGAGGTGGTGGACAGCTGAATGTGCCTCCCGGGCGTTGGCTTACGGCTCCGTTTAATCTCACTAGCCATATGACTTTGTTTTTAGATGAAGATGCTGTTATATTAGGGGTTCAGGTGAGTTTTTCCTTTCTTCCTTTCTTTTGGATGCTCTCTTTCATCTTGAATCTGGTTAAATTCTTAGTCTGAAGAGTTTAGGAGTGAATTAGTTTTGACTGGATTTTTGATACTGAGAATAACATGATAAAATGGTTAGTATTTTCCGTTCAAACTTGCATGCTTTATTGGATGCCATTTAGCTCTGTTTGCACTTACCATTGTAGTTAAAATTGTAAGATGATGCATCAAATAGAATGTTATATTAATAGCGAGCTTCACCATTACCTATTGCTAATTACTATTAGCACTGATACTGATTCTGATACTGATTATGAATAATTTTTTAGACCTGAAGAAGCAAACTGAAAACTGGAAGTTCCAAGTAGAAATGCGTAGTTTAGGTTATGGATGCTATAGAGTGGTAGTTAGATCTTTGAGTATTCTAAAATCAGGAATCTATAAGCCAGTTAGTAAAGTGCCATACCATTTTCCATTCACAATTATGAATGATGAATACTTTCTCATGTTGCGAGAGCTATATGCGGTTGTTTCAGACGATCAGATTAGACATACAGGTGCAACAATATGAATTCTGTGTTCACTTGTCCGATTGTCTGTTTGAACTGACTGCCGAGGTTTGCCCTCACGAATGGGATTATTTTTGTACTCACGTCCCCATCTTTTCCATGGAATAGGTGGTAATAGTAATGCTAAATTGTTTTCTAGACCGATTGCTTTGCTGCTCCTTAAAGGTTTTCGCACGGCAACATGGTATCTTTATGGTTTTTTGGTACTTTGACATTTTAACTACTATGCAACTTATCAGACATCGGTCTTTAGTTAAACAAATGGATTAGTTTGAATTTTTGTTCAACATGGATTAACACTGTGCTTTAATAATTTATTCTTCACCATAAAATGAGCCTCTAGTGACAGGTGGAATGGTAGCTGATCTTTTTCCTGCCACAGGATGAAAAATATTGGCCACTGATGCCTCCTTTGCCTTCCTATGGGTATGGAAGAGAGCACATTGGACCTCGGTACGGGAGTTTGATTCACGGTCAAAATCTCAGAGATGTTGTGATAACAGGTCAGATTGGTTAGCCTTTCATAATCTAATTACTTTTTTATTAGCCTTGATTTGGAATCTAATGTCAACATAATGTGTTAACATATCAAGAAAGTGCAAATTATCATCACTTGAAGAGACGCAGATGCAGATAATGAATTGCAAACAAGAAATTTCTACAGTCTCCATTAAATATCTGTTTTCTAACTTCTAGATACTATAGCATGATTACGTAGAGTGACGGCTAGATGTAACCTGTTGAAGCTTCAATTTTGAATAATAGACAAAGCTGCAGATTAAAGAGGGAAATTAGGAGTAAAGAGAGATTGGAATAACTGAAAACAGGGAAAAGTAGAAGAGGGGAAAGAAAACTCATTAACTGAATTCATCTTCCATATATAATTATCCATACAGGAGGAAAAAAACTAAAACATAAGCATCCAACTCAAAACAACAGATAAGGGGAAAAAAGGCACGACAACCCTAGGGAGAAAATTGCGACGAAAAGCTGCATGATACAAACTGCACTATAACACAACGACCGATATCCCAAGAAGCCCTAATCTTCGATTCCTGACTTCTGATTGTGTATATGTCGTGACTTACAGGACATAATGGTACTATAAACGGACAGGGTCAAACCTGGTGGAAGAAATATCGCCAGAAGCTTCTTAACCACACAAGGGGTCCACTCGTTCAGATCATGTGGTCAAGTGATATTGTGATTAGCAATATTACTTTGCGTGACTCTCCTTTTTGGACACTTCATCCATATGACTGTAAGAATGTTACTATCCGGAAAGTTACGATCTTGGCTCCTGTACACGATGCACCAAATACAGATGGAATAGATCCCGGTAAATTTTTATGCTTTGTTTATATCATTGTCCAATATGATGCTTGGTTTACTGTAAATTGTTATGATATGGCTGCCTGATGGGTACCTCTAGTTGACATCGATATATTGCATCAGTTTTCGTATGAGATGGGTACCTCAAATTAAGAAATCACAGACCATTTAGCTTTTCCTGTAGTATAATTTTGTGTGTAGAAGGTGAAACTGAAACAAAGTTTTTCTAGATTAGGTGGCACCGGCTTAAAGATTTGAAAAATAAACGAGTTTTGAGGGGCACAATGATGAACAACTGCCCGCTGGGTTTGTTTCGTTGCATAGCCTATGTAAGTTGAAAGCATAAGAAAATCCTTCTGGGTTGAGGTCGAACCAAGGGAGTGATTGAACTTAAATTGCACTGGGTTTTAGATCATCTGTTATAAGGTCAAATTTATTAGTCTACCATAGATTTAGATACACGCAACTTTATTTGTTGTCATTTGATAACAACTCGTTTCATATTCTTTATATTGTCATAAACAGATTCATGTGAAGATATGCTGATTGAGGATTGTTACATAAGCGTTGGAGACGATGGCATCGCAATTAAAAGCGGTTGGGATCAGTATGGCATTGCTTATGGGCAACCCTCAAGAAACATACGAATCCGAAATGTTGTTCTTCAATCTATGGTCAGGTAAACATCTTGCACGTGTTTGCGTGACAAATACATATTGACTGCATTCATGTGTCTAGATATCTCTCTCCCTTTATGAGGAAGGAGCAAATGGAACTTTCTGTTATCTGTCAATTCTGCAGTCGGTTACTTTTATAGATTAGATTGTAGCTGAGTTACCACTTTGGTTGAAATCCTTCAGCGCTGGTATATCAATTGGTAGTGAGATGTCGGGTGGCGTATCGGGCGTCACTGTGGAGAATGTCGTGGTGTGGAACTCCAGGCGTGGAGTTCGGATCAAGACTGCTCCAGGAAGAGGGGGATACGTGCGAGACATCACTTATCGAAATCTGACCCTCGACGTTGTTCGTGTTGGGATTGTCATAAAGACAGACTACAATGAACACCCAGACGAGGGATTCGATCCAAAGGCAGTTCCAGTACTGAAGGACATAAGCTTCACAAGCATCCACGGGCAGGGAGTTCGAGTCCCCGTTCGGATGCACGGTAGCGAAGAGATTCCAGTGAGAAACGTGACATTCAGGGACATGTCAGTGGGTATAACATACAAGAAGAAGCATATATTCCAATGTGCATTCGTTCATGGGCGTGTCATAGGAACGATCTTCCCAGCTCCTTGTGACAACCTCGACCGATACGACGAGCAAGAACGGCTAATTAAACGCTCTGCCTCCCAGAATGCAACAGATATCGATTACGACTTT

mRNA sequence

ATGATGGTGGAGACGTCAATTCTGGGTGGGCGGTTCCATAGCCAGAGAATTGACTTCAAGCGATGCATTCCGGCTTTCTTCTCCACCTACAGGACGCTCTTTACAGTGTTCTGGATCGCGGCGGTTGCCACGATTTTTCTCTGGCAGGGTACTATAGGTAATGGGTTCTTGTCGCTCCGGCGAGCCTCCGCCAGACCGTTGCCGCGGCTGAGGCCTGTTGCGTTCAATTTGACGGATTTTGGAGCAGTGGGCGACGGGGTGACATTGAATACCGAGGCCTTTGAGAGGGCTATTTTGGCTATTTCGAAGTTGAGGACCAGAGGTGGTGGACAGCTGAATGTGCCTCCCGGGCGTTGGCTTACGGCTCCGTTTAATCTCACTAGCCATATGACTTTGTTTTTAGATGAAGATGCTGTTATATTAGGGGTTCAGGATGAAAAATATTGGCCACTGATGCCTCCTTTGCCTTCCTATGGGTATGGAAGAGAGCACATTGGACCTCGGTACGGGAGTTTGATTCACGGTCAAAATCTCAGAGATGTTGTGATAACAGGACATAATGGTACTATAAACGGACAGGGTCAAACCTGGTGGAAGAAATATCGCCAGAAGCTTCTTAACCACACAAGGGGTCCACTCGTTCAGATCATGTGGTCAAGTGATATTGTGATTAGCAATATTACTTTGCGTGACTCTCCTTTTTGGACACTTCATCCATATGACTGTAAGAATGTTACTATCCGGAAAGTTACGATCTTGGCTCCTGTACACGATGCACCAAATACAGATGGAATAGATCCCGATTCATGTGAAGATATGCTGATTGAGGATTGTTACATAAGCGTTGGAGACGATGGCATCGCAATTAAAAGCGGTTGGGATCAGTATGGCATTGCTTATGGGCAACCCTCAAGAAACATACGAATCCGAAATGTTGTTCTTCAATCTATGGTCAGCGCTGGTATATCAATTGGTAGTGAGATGTCGGGTGGCGTATCGGGCGTCACTGTGGAGAATGTCGTGGTGTGGAACTCCAGGCGTGGAGTTCGGATCAAGACTGCTCCAGGAAGAGGGGGATACGTGCGAGACATCACTTATCGAAATCTGACCCTCGACGTTGTTCGTGTTGGGATTGTCATAAAGACAGACTACAATGAACACCCAGACGAGGGATTCGATCCAAAGGCAGTTCCAGTACTGAAGGACATAAGCTTCACAAGCATCCACGGGCAGGGAGTTCGAGTCCCCGTTCGGATGCACGGTAGCGAAGAGATTCCAGTGAGAAACGTGACATTCAGGGACATGTCAGTGGGTATAACATACAAGAAGAAGCATATATTCCAATGTGCATTCGTTCATGGGCGTGTCATAGGAACGATCTTCCCAGCTCCTTGTGACAACCTCGACCGATACGACGAGCAAGAACGGCTAATTAAACGCTCTGCCTCCCAGAATGCAACAGATATCGATTACGACTTT

Coding sequence (CDS)

ATGATGGTGGAGACGTCAATTCTGGGTGGGCGGTTCCATAGCCAGAGAATTGACTTCAAGCGATGCATTCCGGCTTTCTTCTCCACCTACAGGACGCTCTTTACAGTGTTCTGGATCGCGGCGGTTGCCACGATTTTTCTCTGGCAGGGTACTATAGGTAATGGGTTCTTGTCGCTCCGGCGAGCCTCCGCCAGACCGTTGCCGCGGCTGAGGCCTGTTGCGTTCAATTTGACGGATTTTGGAGCAGTGGGCGACGGGGTGACATTGAATACCGAGGCCTTTGAGAGGGCTATTTTGGCTATTTCGAAGTTGAGGACCAGAGGTGGTGGACAGCTGAATGTGCCTCCCGGGCGTTGGCTTACGGCTCCGTTTAATCTCACTAGCCATATGACTTTGTTTTTAGATGAAGATGCTGTTATATTAGGGGTTCAGGATGAAAAATATTGGCCACTGATGCCTCCTTTGCCTTCCTATGGGTATGGAAGAGAGCACATTGGACCTCGGTACGGGAGTTTGATTCACGGTCAAAATCTCAGAGATGTTGTGATAACAGGACATAATGGTACTATAAACGGACAGGGTCAAACCTGGTGGAAGAAATATCGCCAGAAGCTTCTTAACCACACAAGGGGTCCACTCGTTCAGATCATGTGGTCAAGTGATATTGTGATTAGCAATATTACTTTGCGTGACTCTCCTTTTTGGACACTTCATCCATATGACTGTAAGAATGTTACTATCCGGAAAGTTACGATCTTGGCTCCTGTACACGATGCACCAAATACAGATGGAATAGATCCCGATTCATGTGAAGATATGCTGATTGAGGATTGTTACATAAGCGTTGGAGACGATGGCATCGCAATTAAAAGCGGTTGGGATCAGTATGGCATTGCTTATGGGCAACCCTCAAGAAACATACGAATCCGAAATGTTGTTCTTCAATCTATGGTCAGCGCTGGTATATCAATTGGTAGTGAGATGTCGGGTGGCGTATCGGGCGTCACTGTGGAGAATGTCGTGGTGTGGAACTCCAGGCGTGGAGTTCGGATCAAGACTGCTCCAGGAAGAGGGGGATACGTGCGAGACATCACTTATCGAAATCTGACCCTCGACGTTGTTCGTGTTGGGATTGTCATAAAGACAGACTACAATGAACACCCAGACGAGGGATTCGATCCAAAGGCAGTTCCAGTACTGAAGGACATAAGCTTCACAAGCATCCACGGGCAGGGAGTTCGAGTCCCCGTTCGGATGCACGGTAGCGAAGAGATTCCAGTGAGAAACGTGACATTCAGGGACATGTCAGTGGGTATAACATACAAGAAGAAGCATATATTCCAATGTGCATTCGTTCATGGGCGTGTCATAGGAACGATCTTCCCAGCTCCTTGTGACAACCTCGACCGATACGACGAGCAAGAACGGCTAATTAAACGCTCTGCCTCCCAGAATGCAACAGATATCGATTACGACTTT

Protein sequence

MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLRRASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWLTAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRDVVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGYVRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMHGSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKRSASQNATDIDYDF

Homology

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BLAST of MS023152.1 vs. NCBI nr
Match: XP_022147646.1 (probable polygalacturonase [Momordica charantia])

HSP 1 Score: 1023.8 bits (2646), Expect = 4.7e-295
Identity = 492/493 (99.80%), Postives = 493/493 (100.00%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR
Sbjct: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL
Sbjct: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAV+LGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVVLGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR
Sbjct: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. NCBI nr
Match: XP_038879850.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 979.5 bits (2531), Expect = 1.0e-281
Identity = 463/493 (93.91%), Postives = 481/493 (97.57%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSILGGRF++QRID KRC+PAFFSTYRTLFTVFWIAAVATIFLWQ TIG+GF  LR
Sbjct: 1   MMVETSILGGRFNNQRIDLKRCVPAFFSTYRTLFTVFWIAAVATIFLWQSTIGDGFFFLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           R SARPLP+LRPV FNLTDFGAVGDGVTLNT+AFE+AILAISKLRT+GGGQLNVP GRWL
Sbjct: 61  RVSARPLPKLRPVVFNLTDFGAVGDGVTLNTKAFEKAILAISKLRTKGGGQLNVPSGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDE+AVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDENAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           V+ITGHNGTINGQGQ WWKKYRQKLLNHTRGPLVQIMWS DIVISNITLRDSPFWTLHPY
Sbjct: 181 VMITGHNGTINGQGQAWWKKYRQKLLNHTRGPLVQIMWSRDIVISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPS+NIRIRNV+LQSMVSAGISIGSEMSGGVSGVTVEN+V+WNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSKNIRIRNVILQSMVSAGISIGSEMSGGVSGVTVENIVIWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DITYRNLTLD VRVGIVIKTDYNEHPDEG+DPKAVPVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDITYRNLTLDTVRVGIVIKTDYNEHPDEGYDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSE+IPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIK 
Sbjct: 421 GSEDIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKH 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. NCBI nr
Match: XP_008450948.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >TYK10010.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 977.2 bits (2525), Expect = 5.1e-281
Identity = 463/493 (93.91%), Postives = 479/493 (97.16%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSI GGRF++QRID KRC+PAFFSTYRT+FTVFWIAAVATIFLWQ TIG+GF  LR
Sbjct: 1   MMVETSIFGGRFNNQRIDLKRCVPAFFSTYRTVFTVFWIAAVATIFLWQSTIGDGFFFLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           R SARPLPRLRP  FNLTDFGAVGDGVTLNT AFE+AILAISKLRT+GGGQLNVP GRWL
Sbjct: 61  RVSARPLPRLRPAVFNLTDFGAVGDGVTLNTNAFEKAILAISKLRTKGGGQLNVPAGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTI+GQGQTWWKKYRQKLLNHTRGPLVQIMWS DI+ISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTISGQGQTWWKKYRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPS+NIRIRNVVLQSMVSAGISIGSEMSGGV G+TVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSKNIRIRNVVLQSMVSAGISIGSEMSGGVLGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DITYRNLTLD VRVGIVIKTDYNEHPDEG+DPKA+PVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDITYRNLTLDTVRVGIVIKTDYNEHPDEGYDPKALPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSE+IPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR
Sbjct: 421 GSEDIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. NCBI nr
Match: KAA0055759.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 977.2 bits (2525), Expect = 5.1e-281
Identity = 462/493 (93.71%), Postives = 479/493 (97.16%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSI GGRF++QRID KRC+PAFFSTYRT+FTVFWIAAVATIFLWQ TIG+GF  LR
Sbjct: 1   MMVETSIFGGRFNNQRIDLKRCVPAFFSTYRTVFTVFWIAAVATIFLWQSTIGDGFFLLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           R SARPLPRLRP  FNLTDFGAVGDGVTLNT AFE+AILAISKLRT+GGGQLNVP GRWL
Sbjct: 61  RVSARPLPRLRPAVFNLTDFGAVGDGVTLNTNAFEKAILAISKLRTKGGGQLNVPAGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTI+GQGQTWWKKYRQKLLNHTRGPL QIMWS DI+ISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTISGQGQTWWKKYRQKLLNHTRGPLFQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPS+NIRIRNVVLQSMVSAGISIGSEMSGG+SG+TVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSKNIRIRNVVLQSMVSAGISIGSEMSGGISGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DITYRNLTLD VRVGIVIKTDYNEHPDEG+DPKA+PVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDITYRNLTLDTVRVGIVIKTDYNEHPDEGYDPKALPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSE+IPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR
Sbjct: 421 GSEDIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. NCBI nr
Match: XP_022987942.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 976.5 bits (2523), Expect = 8.7e-281
Identity = 465/493 (94.32%), Postives = 481/493 (97.57%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSILGGRF++QRIDFKRC+PAFFSTYRTLFTV WIAAVATIFLW  TI +GF  LR
Sbjct: 1   MMVETSILGGRFNNQRIDFKRCVPAFFSTYRTLFTVLWIAAVATIFLWMSTIRDGFFFLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
             S+RPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLR++GGGQLNVP GRWL
Sbjct: 61  PVSSRPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRSKGGGQLNVPSGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWS DIVISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSRDIVISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
            QPS+NIRIRNVVLQSMVSAGISIGSEMSGGVSG+TVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 RQPSKNIRIRNVVLQSMVSAGISIGSEMSGGVSGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DI+YRNLTLDVVRVGIVIKTDYNEHPDEG+DPKAVPVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDISYRNLTLDVVRVGIVIKTDYNEHPDEGYDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFV+GRVIGTIFPAPCDNLDRYDEQERL+KR
Sbjct: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVYGRVIGTIFPAPCDNLDRYDEQERLVKR 480

Query: 481 SASQNATDIDYDF 494
           SA+QNATDIDYDF
Sbjct: 481 SAAQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 3.2e-108
Identity = 188/394 (47.72%), Postives = 260/394 (65.99%), Query Frame = 0


Query: 76  NLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWLTAPFNLTSHMTLFLD 135
           +L DFG VGDG TLNT+AF+ A+  +SK  + GG QL VP G+WLT  F+LTSH TLFL 
Sbjct: 64  SLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLH 123

Query: 136 EDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRDVVITGHNGTINGQGQ 195
            DAV+L  QD   WP++ PLPSYG GR+    RY SLI G NL DV+ITG NGTI+GQG 
Sbjct: 124 RDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGG 183

Query: 196 TWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIRKVTILAP 255
            WW+++    L +TR  L+++M+S+DI ISN+TL +SP W +HP   +N+ I+ +TILAP
Sbjct: 184 LWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAP 243

Query: 256 VHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNVVLQ 315
           V  +PNTDGI+PDSC +  IEDCYI  GDD +A+KSGWD+YGIAYG P++ + IR +   
Sbjct: 244 VR-SPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCI 303

Query: 316 SMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGYVRDITYRNLTLDVVR 375
           S  SA I++GSEMSGG+  V  E++V  NS  G+RIKT  GRGGYV+DI  R +T+  ++
Sbjct: 304 SPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMK 363

Query: 376 VGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMHGSEEIPVRNVTFRDM 435
               +  +Y  H D  +DPKA PV++ I++  +  + V +  R+ G    P   +   ++
Sbjct: 364 WAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNV 423

Query: 436 SVGITYKKKHI-FQCAFVHGRVIGTIFPAPCDNL 469
           ++ +  K K + + C  V G +   + P PC  L
Sbjct: 424 TIHLAAKAKKVPWTCTDVEG-ISSGVTPTPCSTL 455

[Close]

BLAST of MS023152.1 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 6.9e-31
Identity = 128/379 (33.77%), Postives = 174/379 (45.91%), Query Frame = 0


Query: 74  AFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWLTAPFNLTSHMTLF 133
           A ++T FGAVGDGVT +++AF +A  A+      G GQ  VP G            MT  
Sbjct: 23  ALDVTQFGAVGDGVTDDSQAFLKAWEAVCS--GTGDGQFVVPAG------------MTFM 82

Query: 134 LDEDAVILGVQDE-KYWPLMPPL------PSYGYGREHIGPRYGSLIHGQNLRDVVITGH 193
           L      L  Q   K  P+   +      PS G  +   G +    I   ++  +VI G 
Sbjct: 83  LQP----LKFQGSCKSTPVFVQMLGKLVAPSKGNWK---GDK-DQWILFTDIEGLVIEG- 142

Query: 194 NGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVT 253
           +G INGQG +WW+         +R   ++    +++ +S +T  DSP   +H  +C  VT
Sbjct: 143 DGEINGQGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVT 202

Query: 254 IRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRN 313
           I  + I AP   +PNTDGID  +  +++I+DC I+ GDD IAI SG           + N
Sbjct: 203 ISSLRINAP-ESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSN 262

Query: 314 IRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWN-----SRRGVRIKTAPGRGGYV 373
           I I  +        GISIGS    G +  TVENV V N     +  G RIKT  G  GY 
Sbjct: 263 IHISGI--DCGPGHGISIGSLGKDGET-ATVENVCVQNCNFRGTMNGARIKTWQGGSGYA 322

Query: 374 RDITYRNLTLDVVRVGIVIKTDYN-EHPDEGFDPK--AVPVLKDISFTSIHGQGVRVPVR 433
           R IT+  +TLD V   I+I   YN    D   D K  AV V K +    I        V 
Sbjct: 323 RMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVD 357

Query: 434 MHGSEEIPVRNVTFRDMSV 438
              SE +P   +  RDM +
Sbjct: 383 FRCSERVPCTEIFLRDMKI 357

[Close]

BLAST of MS023152.1 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 2.3e-26
Identity = 107/372 (28.76%), Postives = 164/372 (44.09%), Query Frame = 0


Query: 55  GFLSLRRASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNV 114
           G  S  RAS R      P   +++DFGA GDG T +T+AF  A        + G   L V
Sbjct: 53  GLRSFVRASLR-----TPTTVSVSDFGAKGDGKTDDTQAFVNAWK--KACSSNGAVNLLV 112

Query: 115 PPGR-------WLTAPFNLTSHMTLF--LDEDAVILGVQDEKYWPLMPPLPSYGYGREHI 174
           P G         LT P N    + +F  L         +D   W +   +          
Sbjct: 113 PKGNTYLLKSIQLTGPCNSILTVQIFGTLSASQKRSDYKDISKWIMFDGV---------- 172

Query: 175 GPRYGSLIHGQNLRDVVITGHNGTINGQGQTWWKK--YRQKLLNHTRGPLVQIMWSS-DI 234
                      NL   V  G  G ++G G+TWW+    R K    T+ P     ++S  +
Sbjct: 173 ----------NNLS--VDGGDTGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSL 232

Query: 235 VISNITLRDSPFWTLHPYDCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISV 294
           ++ N+ +R++    +    C NV +  V + AP  D+PNTDGI   + +++ + +  I  
Sbjct: 233 IVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPA-DSPNTDGIHITNTQNIRVSESIIGT 292

Query: 295 GDDGIAIKSGWDQYGIAYGQPSRNIRIRNVVLQSMVSAGISIGS----EMSGGVSGVTVE 354
           GDD I+I+SG           S+N++I ++        GISIGS         VSGVTV+
Sbjct: 293 GDDCISIESG-----------SQNVQINDITCGP--GHGISIGSLGDDNSKAFVSGVTVD 352

Query: 355 NVVVWNSRRGVRIKTAPGRGGYVRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVP 411
              +  +  GVRIKT  G  G   +I ++N+ +D V+  I+I  DY +      +  AV 
Sbjct: 353 GAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ 380

[Close]

BLAST of MS023152.1 vs. ExPASy Swiss-Prot
Match: P27644 (Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 8.2e-24
Identity = 66/161 (40.99%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 228 TLRDSPFWTLHPYDCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGI 287
           T+R++  WT+HP  C+++T    TI+AP HD+PNTDG +P+SC +++I     SVGDD I
Sbjct: 39  TIRNAASWTIHPQGCEDLTAAASTIIAP-HDSPNTDGFNPESCRNVMISGVRFSVGDDCI 98

Query: 288 AIKSG--WDQYGIAYGQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNS 347
           A+K+G         +   +R I +R+ ++Q     G+ IGSEMSGGV  VTVE+  +  +
Sbjct: 99  AVKAGKRGPDGEDDHLAETRGITVRHCLMQP-GHGGLVIGSEMSGGVHDVTVEDCDMIGT 158

Query: 348 RRGVRIKT-APGRGGYVRDITYRNLTLDVVRVGIVIKTDYN 386
            RG+R+KT A   GG V +IT R + LD V+  +     Y+
Sbjct: 159 DRGLRLKTGARSGGGMVGNITMRRVLLDGVQTALSANAHYH 197

[Close]

BLAST of MS023152.1 vs. ExPASy Swiss-Prot
Match: P18192 (Endo-polygalacturonase OS=Pectobacterium carotovorum subsp. carotovorum OX=555 GN=peh PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-20
Identity = 100/330 (30.30%), Postives = 154/330 (46.67%), Query Frame = 0


Query: 119 WLTAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNL 178
           +L+ P +L S ++L +D+   +  V + K +   P   S G    + G    + I   + 
Sbjct: 78  FLSGPLSLPSGVSLLIDKGVTLRAVNNAKSFENAP--SSCGVVDTN-GKGCDAFITATST 137

Query: 179 RDVVITGHNGTINGQG--------QTWW---KKYRQKLLNHTRGPLVQIMWSSDIVISNI 238
            +  I G  GTI+GQG         +WW      + K L      L+QI  S +  + N+
Sbjct: 138 TNSGIYG-PGTIDGQGGVKLQDKKVSWWDLAADAKVKKLKQNTPRLIQINKSKNFTLYNV 197

Query: 239 TLRDSPFWTLHPYDCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGI 298
           +L +SP + +   D    T  K TI  P   A NTDGIDP S +++ I    IS GDD +
Sbjct: 198 SLINSPNFHVVFSDGDGFTAWKTTIKTP-STARNTDGIDPMSSKNITIAHSNISTGDDNV 257

Query: 299 AIKSGWDQYGIAYGQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRR 358
           AIK        AY   S    I  +  +     G+SIGSE + GV  VTV+++++  +  
Sbjct: 258 AIK--------AYKGRSETRNISILHNEFGTGHGMSIGSE-TMGVYNVTVDDLIMTGTTN 317

Query: 359 GVRIKTAPGRGGYVRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTS 418
           G+RIK+     G V  + Y N+ +  V   IVI T Y +   EG +   VP   DI+F  
Sbjct: 318 GLRIKSDKSAAGVVNGVRYSNVVMKNVAKPIVIDTVYEK--KEGSN---VPDWSDITFKD 377

Query: 419 IHGQGVRVPVRMHGSEEIPVRNVTFRDMSV 438
           I  Q   V V    + + P+  VT +++ +
Sbjct: 378 ITSQTKGVVVLNGENAKKPI-EVTMKNVKL 387

[Close]

BLAST of MS023152.1 vs. ExPASy TrEMBL
Match: A0A6J1D302 (probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111016517 PE=3 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.3e-295
Identity = 492/493 (99.80%), Postives = 493/493 (100.00%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR
Sbjct: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL
Sbjct: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAV+LGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVVLGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR
Sbjct: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. ExPASy TrEMBL
Match: A0A5D3CF90 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001410 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.5e-281
Identity = 463/493 (93.91%), Postives = 479/493 (97.16%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSI GGRF++QRID KRC+PAFFSTYRT+FTVFWIAAVATIFLWQ TIG+GF  LR
Sbjct: 1   MMVETSIFGGRFNNQRIDLKRCVPAFFSTYRTVFTVFWIAAVATIFLWQSTIGDGFFFLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           R SARPLPRLRP  FNLTDFGAVGDGVTLNT AFE+AILAISKLRT+GGGQLNVP GRWL
Sbjct: 61  RVSARPLPRLRPAVFNLTDFGAVGDGVTLNTNAFEKAILAISKLRTKGGGQLNVPAGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTI+GQGQTWWKKYRQKLLNHTRGPLVQIMWS DI+ISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTISGQGQTWWKKYRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPS+NIRIRNVVLQSMVSAGISIGSEMSGGV G+TVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSKNIRIRNVVLQSMVSAGISIGSEMSGGVLGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DITYRNLTLD VRVGIVIKTDYNEHPDEG+DPKA+PVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDITYRNLTLDTVRVGIVIKTDYNEHPDEGYDPKALPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSE+IPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR
Sbjct: 421 GSEDIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. ExPASy TrEMBL
Match: A0A5A7UQK3 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001450 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.5e-281
Identity = 462/493 (93.71%), Postives = 479/493 (97.16%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSI GGRF++QRID KRC+PAFFSTYRT+FTVFWIAAVATIFLWQ TIG+GF  LR
Sbjct: 1   MMVETSIFGGRFNNQRIDLKRCVPAFFSTYRTVFTVFWIAAVATIFLWQSTIGDGFFLLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           R SARPLPRLRP  FNLTDFGAVGDGVTLNT AFE+AILAISKLRT+GGGQLNVP GRWL
Sbjct: 61  RVSARPLPRLRPAVFNLTDFGAVGDGVTLNTNAFEKAILAISKLRTKGGGQLNVPAGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTI+GQGQTWWKKYRQKLLNHTRGPL QIMWS DI+ISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTISGQGQTWWKKYRQKLLNHTRGPLFQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPS+NIRIRNVVLQSMVSAGISIGSEMSGG+SG+TVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSKNIRIRNVVLQSMVSAGISIGSEMSGGISGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DITYRNLTLD VRVGIVIKTDYNEHPDEG+DPKA+PVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDITYRNLTLDTVRVGIVIKTDYNEHPDEGYDPKALPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSE+IPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR
Sbjct: 421 GSEDIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. ExPASy TrEMBL
Match: A0A1S3BR46 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103492385 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.5e-281
Identity = 463/493 (93.91%), Postives = 479/493 (97.16%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSI GGRF++QRID KRC+PAFFSTYRT+FTVFWIAAVATIFLWQ TIG+GF  LR
Sbjct: 1   MMVETSIFGGRFNNQRIDLKRCVPAFFSTYRTVFTVFWIAAVATIFLWQSTIGDGFFFLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
           R SARPLPRLRP  FNLTDFGAVGDGVTLNT AFE+AILAISKLRT+GGGQLNVP GRWL
Sbjct: 61  RVSARPLPRLRPAVFNLTDFGAVGDGVTLNTNAFEKAILAISKLRTKGGGQLNVPAGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTI+GQGQTWWKKYRQKLLNHTRGPLVQIMWS DI+ISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTISGQGQTWWKKYRQKLLNHTRGPLVQIMWSRDILISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
           GQPS+NIRIRNVVLQSMVSAGISIGSEMSGGV G+TVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 GQPSKNIRIRNVVLQSMVSAGISIGSEMSGGVLGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DITYRNLTLD VRVGIVIKTDYNEHPDEG+DPKA+PVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDITYRNLTLDTVRVGIVIKTDYNEHPDEGYDPKALPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSE+IPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR
Sbjct: 421 GSEDIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480

Query: 481 SASQNATDIDYDF 494
           SASQNATDIDYDF
Sbjct: 481 SASQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. ExPASy TrEMBL
Match: A0A6J1JBR3 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111485343 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.2e-281
Identity = 465/493 (94.32%), Postives = 481/493 (97.57%), Query Frame = 0


Query: 1   MMVETSILGGRFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQGTIGNGFLSLR 60
           MMVETSILGGRF++QRIDFKRC+PAFFSTYRTLFTV WIAAVATIFLW  TI +GF  LR
Sbjct: 1   MMVETSILGGRFNNQRIDFKRCVPAFFSTYRTLFTVLWIAAVATIFLWMSTIRDGFFFLR 60

Query: 61  RASARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWL 120
             S+RPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLR++GGGQLNVP GRWL
Sbjct: 61  PVSSRPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRSKGGGQLNVPSGRWL 120

Query: 121 TAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180
           TAPFNLTSHMTLFLDEDAVILG+QDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD
Sbjct: 121 TAPFNLTSHMTLFLDEDAVILGIQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRD 180

Query: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPY 240
           VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWS DIVISNITLRDSPFWTLHPY
Sbjct: 181 VVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSRDIVISNITLRDSPFWTLHPY 240

Query: 241 DCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300
           DCKNVTIR VTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY
Sbjct: 241 DCKNVTIRNVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAY 300

Query: 301 GQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGY 360
            QPS+NIRIRNVVLQSMVSAGISIGSEMSGGVSG+TVENVVVWNSRRGVRIKTAPGRGGY
Sbjct: 301 RQPSKNIRIRNVVLQSMVSAGISIGSEMSGGVSGITVENVVVWNSRRGVRIKTAPGRGGY 360

Query: 361 VRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMH 420
           V+DI+YRNLTLDVVRVGIVIKTDYNEHPDEG+DPKAVPVLKDISFTSIHGQGVRVPVRMH
Sbjct: 361 VQDISYRNLTLDVVRVGIVIKTDYNEHPDEGYDPKAVPVLKDISFTSIHGQGVRVPVRMH 420

Query: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKR 480
           GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFV+GRVIGTIFPAPCDNLDRYDEQERL+KR
Sbjct: 421 GSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVYGRVIGTIFPAPCDNLDRYDEQERLVKR 480

Query: 481 SASQNATDIDYDF 494
           SA+QNATDIDYDF
Sbjct: 481 SAAQNATDIDYDF 493

[Close]

BLAST of MS023152.1 vs. TAIR 10
Match: AT3G42950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 788.1 bits (2034), Expect = 4.1e-228
Identity = 370/479 (77.24%), Postives = 425/479 (88.73%), Query Frame = 0


Query: 26  FFSTYRTLFTVFWIAAVATIFLWQ-----GTIGNGFLSLRRASARP-------LPRLRPV 85
           F S++++L TVFWIA  A++F+WQ       + +GF     +S+          P+LRPV
Sbjct: 5   FLSSHKSLITVFWIATFASLFIWQFGGVSTNLYSGFSLFWSSSSTTTTVFSGGFPKLRPV 64

Query: 86  AFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWLTAPFNLTSHMTLF 145
            FNLTDFGAVGDGVT+NTEAFE+AI  ISKL  +GGGQLNVPPGRWLTAPFNLTS+MTLF
Sbjct: 65  VFNLTDFGAVGDGVTINTEAFEKAIYKISKLAKKGGGQLNVPPGRWLTAPFNLTSYMTLF 124

Query: 146 LDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRDVVITGHNGTINGQ 205
           L E+A IL +QDEKYW L+PPLPSYGYGREH GPRYGS IHGQNLRDVV+TG+NG+INGQ
Sbjct: 125 LSENAEILALQDEKYWSLLPPLPSYGYGREHHGPRYGSFIHGQNLRDVVVTGNNGSINGQ 184

Query: 206 GQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIRKVTIL 265
           GQTWWKKYRQKLLNHTRGPLVQIMWSSDIV +NITLRDSPFWTLHPYDCKNVTI  +TIL
Sbjct: 185 GQTWWKKYRQKLLNHTRGPLVQIMWSSDIVFANITLRDSPFWTLHPYDCKNVTITNMTIL 244

Query: 266 APVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNVV 325
           APV +APNTDGIDPDSCEDMLIE+ YISVGDDGIAIKSGWDQYG  YG+PS+NI IRN++
Sbjct: 245 APVFEAPNTDGIDPDSCEDMLIENSYISVGDDGIAIKSGWDQYGTTYGKPSKNILIRNLI 304

Query: 326 LQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGYVRDITYRNLTLDV 385
           ++SMVSAGISIGSEMSGGVS +TVEN+++W+SRRGVRIKTAPGRGGYVRDIT+RN+TLD 
Sbjct: 305 IRSMVSAGISIGSEMSGGVSNITVENILIWSSRRGVRIKTAPGRGGYVRDITFRNVTLDE 364

Query: 386 VRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMHGSEEIPVRNVTFR 445
           +RVGIVIKTDYNEHPD GF+P+A P+L++I++T I+GQGVRVPVR+ GS+EIPV+NVTFR
Sbjct: 365 LRVGIVIKTDYNEHPDGGFNPQAFPILENINYTGIYGQGVRVPVRIQGSKEIPVKNVTFR 424

Query: 446 DMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERLIKRSASQNATDIDYD 493
           DMSVGITYKKKHIFQCA+V GRVIGTIFPAPCDNLDRYDEQERL+K+S SQNATDIDY+
Sbjct: 425 DMSVGITYKKKHIFQCAYVEGRVIGTIFPAPCDNLDRYDEQERLVKQSDSQNATDIDYE 483

[Close]

BLAST of MS023152.1 vs. TAIR 10
Match: AT1G19170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 777.3 bits (2006), Expect = 7.2e-225
Identity = 361/495 (72.93%), Postives = 422/495 (85.25%), Query Frame = 0


Query: 11  RFHSQRIDFKRCIPAFFSTYRTLFTVFWIAAVATIFLWQ-----------GTIGNGFLSL 70
           +FH QR+D KR + +  ++++TLFT  WI A  ++FLWQ           G +G  F   
Sbjct: 11  QFHLQRLDPKRRLTSLLASHKTLFTFLWITAFGSVFLWQRTAYIEGGSGTGPVGGKFTIF 70

Query: 71  RRAS-ARPLPRLRPVAFNLTDFGAVGDGVTLNTEAFERAILAISKL-RTRGGGQLNVPPG 130
            +    +P+PRLRPV F+L DFG VGDG TLNTEAFERA+++ISKL  + GGGQLNVPPG
Sbjct: 71  GKIKPIQPIPRLRPVVFDLKDFGGVGDGFTLNTEAFERAVISISKLGGSSGGGQLNVPPG 130

Query: 131 RWLTAPFNLTSHMTLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQN 190
           RWLTAPFNLTSHMTLFL ED+ ILGV+DEKYWPLMPPLPSYGYGRE  GPRYGSLIHGQN
Sbjct: 131 RWLTAPFNLTSHMTLFLAEDSEILGVEDEKYWPLMPPLPSYGYGRERPGPRYGSLIHGQN 190

Query: 191 LRDVVITGHNGTINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTL 250
           L+D+VITGHNGTINGQGQ+WWKK++++LLN+TRGPLVQIMWSSDIVI+NIT+RDSPFWTL
Sbjct: 191 LKDIVITGHNGTINGQGQSWWKKHQRRLLNYTRGPLVQIMWSSDIVIANITMRDSPFWTL 250

Query: 251 HPYDCKNVTIRKVTILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYG 310
           HPYDCKNVTIR VTILAPV  APNTDGIDPDSCEDM+IEDCYIS GDD IAIKSGWDQ+G
Sbjct: 251 HPYDCKNVTIRNVTILAPVTGAPNTDGIDPDSCEDMVIEDCYISTGDDAIAIKSGWDQFG 310

Query: 311 IAYGQPSRNIRIRNVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGR 370
           IAYG+PS NI IRN+V++S++SAG+SIGSEMSGG+S VT+EN+++WNSRRG+RIKTAPGR
Sbjct: 311 IAYGRPSTNILIRNLVVRSVISAGVSIGSEMSGGISNVTIENLLIWNSRRGIRIKTAPGR 370

Query: 371 GGYVRDITYRNLTLDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPV 430
           GGY+R+ITY+NLTLD VRVGIVIKTDYNEH D+ +D KA P+L   SF  IHGQGVRVPV
Sbjct: 371 GGYIRNITYKNLTLDNVRVGIVIKTDYNEHADDNYDRKAYPILSGFSFAGIHGQGVRVPV 430

Query: 431 RMHGSEEIPVRNVTFRDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQERL 490
           R+HGSE+IPVRNVTFRDMSVG+TYKKKHIFQC+FV GRV G+IFP PC+N D YDE+ RL
Sbjct: 431 RIHGSEQIPVRNVTFRDMSVGLTYKKKHIFQCSFVKGRVFGSIFPRPCENFDVYDEEGRL 490

Query: 491 IKRSASQNATDIDYD 493
           +K +A  N  DIDYD
Sbjct: 491 VKPAAESNVADIDYD 505

[Close]

BLAST of MS023152.1 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.8e-114
Identity = 200/402 (49.75%), Postives = 268/402 (66.67%), Query Frame = 0


Query: 77  LTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWLTAPFNLTSHMTLFLDE 136
           LTDFGAVGDG T NT+AF  AI  +S++ T GG QL VPPG+WLT  FNLTSH TLF+  
Sbjct: 47  LTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQR 106

Query: 137 DAVILGVQDEKYWPLMPPLPSYGYGREHIGP-RYGSLIHGQNLRDVVITGHNGTINGQGQ 196
            A IL  QDE  WP++ PLPSYG GR+  G  R+ SLI G NL DVVITG+NGTINGQGQ
Sbjct: 107 GATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQ 166

Query: 197 TWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIRKVTILAP 256
            WW K+++K    TR  L++I++S +I ISNITL DSP W +HP  C +V ++ VT+LAP
Sbjct: 167 YWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAP 226

Query: 257 VHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNVVLQ 316
           V   PNTDGI+PDSC + LIEDCYI  GDD IA+KSGWDQYGI +G P++ + IR +   
Sbjct: 227 V-TVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCI 286

Query: 317 SMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGYVRDITYRNLTLDVVR 376
           S  SAG+++GSEMSGG+  V +E+V + N+   +RIKTA GRG YV+DI  R +T+  ++
Sbjct: 287 SPKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMK 346

Query: 377 VGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMHGSEEIPVRNVTFRDM 436
               +  +Y  HPDEGFDPKA+P + +I++  +  + V +   + G ++ P   +   ++
Sbjct: 347 YVFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNV 406

Query: 437 SVGITYK-KKHIFQCAFVHGRVIGTIFPAPCDNLDRYDEQER 477
           ++ +  K KK  + C  V G V   + P PC  L     Q +
Sbjct: 407 TIALAAKAKKMQWNCTDVAG-VTSRVTPEPCSLLPEKKAQAK 446

[Close]

BLAST of MS023152.1 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 407.9 bits (1047), Expect = 1.1e-113
Identity = 193/399 (48.37%), Postives = 280/399 (70.18%), Query Frame = 0


Query: 71  RPVAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWLTAPFNLTSHM 130
           RP + ++T+FGAVGDGVTLNT+AF+ A+  ++    +GG +L VP G+WLT  F+L SH+
Sbjct: 39  RPHSVSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHL 98

Query: 131 TLFLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRDVVITGHNGTI 190
           TL+LD+ A ILG    + WP++ PLPSYG GRE  G R+ SLI+GQNL DVVITG NGTI
Sbjct: 99  TLWLDKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTI 158

Query: 191 NGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIRKV 250
           +GQG  WW  +R   LN+TR  LV++M S+ ++ISN+T  +SPFW +HP  C++V ++ +
Sbjct: 159 DGQGTVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNL 218

Query: 251 TILAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIR 310
           TILAP+ ++PNTDG+DPDS  ++ IEDCYI  GDD ++IKSGWD+YGI+Y +PS  I+I 
Sbjct: 219 TILAPL-ESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKIN 278

Query: 311 NVVLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGYVRDITYRNLT 370
            +  Q+  S+GI+IGSEMSGGVS + ++++ ++NS  G+RIKT+ GRGGYVR++   N+ 
Sbjct: 279 RLTGQTTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVK 338

Query: 371 LDVVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMHGSEEIPVRNV 430
           LD V+  I     Y EHPDE +DPKA+P ++ I+F +++G G+ V   + G E    +N+
Sbjct: 339 LDNVKKAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNI 398

Query: 431 TFRDMSVGI-TYKKKHIFQCAFVHGRVIGTIFPAPCDNL 469
            F ++++ +    KK  ++C+ V G          CD+L
Sbjct: 399 CFLNVTLRVKKNSKKSPWECSNVRGYSQWVSPEITCDSL 436

[Close]

BLAST of MS023152.1 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.6e-110
Identity = 194/396 (48.99%), Postives = 266/396 (67.17%), Query Frame = 0


Query: 73  VAFNLTDFGAVGDGVTLNTEAFERAILAISKLRTRGGGQLNVPPGRWLTAPFNLTSHMTL 132
           +  ++TDFG VGDG T NT AF RA+  +      GG QLNVP G WL+  FNLTS+ TL
Sbjct: 70  IVMSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTL 129

Query: 133 FLDEDAVILGVQDEKYWPLMPPLPSYGYGREHIGPRYGSLIHGQNLRDVVITGHNGTING 192
           FL+  A+ILG +D   WP++ PLPSYG GRE  G R+ SLIHG NL +VVITG NGTI+G
Sbjct: 130 FLERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDG 189

Query: 193 QGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIRKVTI 252
           QG+ WW+ +  + L HTRG L+++  S +I+ISN+TL +SPFWT+HP  C NV IR +TI
Sbjct: 190 QGKMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYCSNVVIRNMTI 249

Query: 253 LAPVHDAPNTDGIDPDSCEDMLIEDCYISVGDDGIAIKSGWDQYGIAYGQPSRNIRIRNV 312
           LAP+ +APNTDGIDPDS  ++ IEDCYI  GDD +A+KSGWDQYG+A  +PS NI IR +
Sbjct: 250 LAPM-NAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARPSSNIVIRRI 309

Query: 313 VLQSMVSAGISIGSEMSGGVSGVTVENVVVWNSRRGVRIKTAPGRGGYVRDITYRNLTLD 372
              +   +G+ IGSEMSGG+  +TVE++ VW+S  G+RIKT  GRGGY+ +IT+ N+ L+
Sbjct: 310 SGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISNITFNNVLLE 369

Query: 373 VVRVGIVIKTDYNEHPDEGFDPKAVPVLKDISFTSIHGQGVRVPVRMHGSEEIPVRNVTF 432
            V+V I   +  N+H D+ +DPKA+P +K I  +++     R    + G E    ++V  
Sbjct: 370 KVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLLGVEGTSFQDVCL 429

Query: 433 RDMSVGITYKKKHIFQCAFVHGRVIGTIFPAPCDNL 469
           R++++ +   K   ++C  V G     +FP  C  L
Sbjct: 430 RNVTL-LGLPKTEKWKCKDVSG-YASDVFPLSCPQL 462

The following BLAST results are available for this feature:

BLAST of MS023152.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147646.1	4.7e-295	99.80	probable polygalacturonase [Momordica charantia]	[more]
XP_038879850.1	1.0e-281	93.91	probable polygalacturonase [Benincasa hispida]	[more]
XP_008450948.1	5.1e-281	93.91	PREDICTED: probable polygalacturonase [Cucumis melo] >TYK10010.1 putative polyga...	[more]
KAA0055759.1	5.1e-281	93.71	putative polygalacturonase [Cucumis melo var. makuwa]	[more]
XP_022987942.1	8.7e-281	94.32	probable polygalacturonase [Cucurbita maxima]	[more]

BLAST of MS023152.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	3.2e-108	47.72	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q9LW07	6.9e-31	33.77	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q8RY29	2.3e-26	28.76	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
P27644	8.2e-24	40.99	Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1	[more]
P18192	1.5e-20	30.30	Endo-polygalacturonase OS=Pectobacterium carotovorum subsp. carotovorum OX=555 G...	[more]

BLAST of MS023152.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D302	2.3e-295	99.80	probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111016517 PE=3 S...	[more]
A0A5D3CF90	2.5e-281	93.91	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7UQK3	2.5e-281	93.71	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3BR46	2.5e-281	93.91	probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103492385 PE=3 SV=1	[more]
A0A6J1JBR3	4.2e-281	94.32	probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111485343 PE=3 SV=1	[more]

BLAST of MS023152.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G42950.1	4.1e-228	77.24	Pectin lyase-like superfamily protein	[more]
AT1G19170.1	7.2e-225	72.93	Pectin lyase-like superfamily protein	[more]
AT3G48950.1	1.8e-114	49.75	Pectin lyase-like superfamily protein	[more]
AT3G62110.1	1.1e-113	48.37	Pectin lyase-like superfamily protein	[more]
AT4G33440.1	2.6e-110	48.99	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 304..326 e-value: 97.0 score: 11.8 coord: 242..269 e-value: 290.0 score: 10.3 coord: 332..353 e-value: 1.3 score: 18.1 coord: 270..291 e-value: 21.0 score: 14.0 coord: 422..443 e-value: 2500.0 score: 4.4 coord: 361..382 e-value: 720.0 score: 8.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 60..454 e-value: 4.1E-125 score: 420.0
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 171..453 e-value: 8.3E-51 score: 173.0
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 23..491
None	No IPR available	PANTHER	PTHR31339:SF44	POLYGALACTURONASE-RELATED	coord: 23..491
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 66..466

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS023152	MS023152	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS023152.1-cds	MS023152.1-cds-scaffold78:484873..485304	CDS
MS023152.1-cds	MS023152.1-cds-scaffold78:486256..486376	CDS
MS023152.1-cds	MS023152.1-cds-scaffold78:486964..487212	CDS
MS023152.1-cds	MS023152.1-cds-scaffold78:487731..487884	CDS
MS023152.1-cds	MS023152.1-cds-scaffold78:488066..488588	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS023152.1	MS023152.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity