MS023140.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS023140.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	guanine nucleotide-binding protein subunit gamma 3
Location	scaffold78: 435958 .. 437666 (-)
Sequence length	651
RNA-Seq Expression	MS023140.1
Synteny	MS023140.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACTCGGTCGGTTTACTCTTCCTCAGTGCCTTCTCTACCGCCCCCGAGTCCCAAGTCTCCGCCCGAGTACCCGGATTTGTATGGGAAGCGTCGTGAGATAGCCAAGATTCAGACGCTTGAAAGAGAAATTGGTTTCCTTGAGGTTAGTTCGAATTTGAATTGATTGTCACTGTCGCCGTCTTCTTCTTCTCCTCCCCCTCCTCCTCCTTCGTCCATCTTTTTCTAGTCCTTTGAAGTTCAATGGCATAGTGACGTCGAGAAGTTGTAGTTCAATTGCAAGCATTTCTCCCTTTGATATTAGCTTAGCTTTGCTACTTAGCAGATTATTGAAGCTCTACATGCAGATCCATTGTTTGATTTGGGGAGCTAGTTTCTCCTTACAGTTAAACAGATTTGATTTGCTCAAAAACTTCCCTTTCATATCATGAAGTTTCTCCTAAAACTTCTTTTTAACTTCTGGAAAAACACCGCCTGGGGAGTCTGGGGCGGGGAAAATTTAAGAAAAATGGTTGGCTCCTAAACTTCCTGGGCTGCCGCTTCCCCAGAGAAAGGGGAATGAAAAAGAAGAAAGAACTGAATTTCTATCCCTTTTTCCAAAATCCGTGAAATTTGAAAATTAGTGGACTCGATGTCTTCTTGCTTTCATTTTCCAGATAATTCATATTTCTTCAGAACTACTTGCGAAAATTTCTCCTCTTTCTAGTCATGGGATCGTTTGGCTGGACAAATGCCACATCTCACATCATAAATTGAAATACAAGTACCATGTTCTTAGATGCGTGAGATGAAACTTACTCTATCACACATGTCAGGAGGCACAGTTTTCAGTTTTAAATTAATTACCATGTCTGCTTTTTATTAAGTTCAGTGTTTTCCTGAAACAGGAAGAACTAAAATCTGTTCAAAGTCTTCAACCCGTCTCAATATGCTGCAAAGAGTATGTTTCTTTTTAATTAATTTCATCATCTTTTAATTTATTAAAGTTCTGAGGTCTTTCTTCTGTTAACTGTACATATTCAATTGGGAAATTTATCCATCCACTTTTTGCTTCTGCTGTTGATTAACATCACACTTTTGCAGGATAAGTGACTTTGTAATGGCCAGCTCAGATCCATTGACACCTACGTAAGGGTTCATTTTTAGTTAAACTAATCTGCAGAAATTTAAGGCCGTACTGATTATGATCCACGTCCATTTTATTTTTCGAGCAGATGTAGGACAAAACGTGAGCGATGTCGCTTCTGGAAATGGTTATGGTTAGAACTTTCAACATATGAATTCTGATTCTCAGCTTGTCACTGAGAATGTTTTTGTGCATAACATTTGTGACGATTGTCATGCAGCGGATTTCCTTGCTTTAACTTGTCGTCACTGTGCTGCTGCTCCTGCGCTGGGTGCTCTGTCTACCTAGAGATGCCTCGCTGCTGTGATTGCAAACAATGTAACTTCCAATCGTGTATAAGCTGTTGTAGCGTGCCAAAGTGTAGTTTTTTCTCTTGTCCCTCTTCCTCCTGCTGTAGGAACATCTCGTGCCGTAGATTGTGTTGCGTTTTATCATTACCATCAATGTCATGCCCCAATCGTTGCTGCTGCTGCGAATGCAAATGCAGCTGTAAGTGCTCTTTTCCAAAATGTCCACAGGTACCTTCTTGTTGCCTCTGTACAAAGACCTGTTGTAATCCCTGCTCTGCATGTCGTTGCTTT

mRNA sequence

ATGGCTACTCGGTCGGTTTACTCTTCCTCAGTGCCTTCTCTACCGCCCCCGAGTCCCAAGTCTCCGCCCGAGTACCCGGATTTGTATGGGAAGCGTCGTGAGATAGCCAAGATTCAGACGCTTGAAAGAGAAATTGGTTTCCTTGAGGAAGAACTAAAATCTGTTCAAAGTCTTCAACCCGTCTCAATATGCTGCAAAGAGATAAGTGACTTTGTAATGGCCAGCTCAGATCCATTGACACCTACATGTAGGACAAAACGTGAGCGATGTCGCTTCTGGAAATGGTTATGCGGATTTCCTTGCTTTAACTTGTCGTCACTGTGCTGCTGCTCCTGCGCTGGGTGCTCTGTCTACCTAGAGATGCCTCGCTGCTGTGATTGCAAACAATGTAACTTCCAATCGTGTATAAGCTGTTGTAGCGTGCCAAAGTGTAGTTTTTTCTCTTGTCCCTCTTCCTCCTGCTGTAGGAACATCTCGTGCCGTAGATTGTGTTGCGTTTTATCATTACCATCAATGTCATGCCCCAATCGTTGCTGCTGCTGCGAATGCAAATGCAGCTGTAAGTGCTCTTTTCCAAAATGTCCACAGGTACCTTCTTGTTGCCTCTGTACAAAGACCTGTTGTAATCCCTGCTCTGCATGTCGTTGCTTT

Coding sequence (CDS)

ATGGCTACTCGGTCGGTTTACTCTTCCTCAGTGCCTTCTCTACCGCCCCCGAGTCCCAAGTCTCCGCCCGAGTACCCGGATTTGTATGGGAAGCGTCGTGAGATAGCCAAGATTCAGACGCTTGAAAGAGAAATTGGTTTCCTTGAGGAAGAACTAAAATCTGTTCAAAGTCTTCAACCCGTCTCAATATGCTGCAAAGAGATAAGTGACTTTGTAATGGCCAGCTCAGATCCATTGACACCTACATGTAGGACAAAACGTGAGCGATGTCGCTTCTGGAAATGGTTATGCGGATTTCCTTGCTTTAACTTGTCGTCACTGTGCTGCTGCTCCTGCGCTGGGTGCTCTGTCTACCTAGAGATGCCTCGCTGCTGTGATTGCAAACAATGTAACTTCCAATCGTGTATAAGCTGTTGTAGCGTGCCAAAGTGTAGTTTTTTCTCTTGTCCCTCTTCCTCCTGCTGTAGGAACATCTCGTGCCGTAGATTGTGTTGCGTTTTATCATTACCATCAATGTCATGCCCCAATCGTTGCTGCTGCTGCGAATGCAAATGCAGCTGTAAGTGCTCTTTTCCAAAATGTCCACAGGTACCTTCTTGTTGCCTCTGTACAAAGACCTGTTGTAATCCCTGCTCTGCATGTCGTTGCTTT

Protein sequence

MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQPVSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLEMPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCCCECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF

Homology

[Close]

BLAST of MS023140.1 vs. NCBI nr
Match: XP_022147636.1 (guanine nucleotide-binding protein subunit gamma 3 [Momordica charantia] >XP_022147637.1 guanine nucleotide-binding protein subunit gamma 3 [Momordica charantia])

HSP 1 Score: 452.2 bits (1162), Expect = 2.5e-123
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP
Sbjct: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE
Sbjct: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC
Sbjct: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF
Sbjct: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 217

[Close]

BLAST of MS023140.1 vs. NCBI nr
Match: XP_038879225.1 (guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_038879226.1 guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_038879227.1 guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_038879228.1 guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_038879229.1 guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_038879230.1 guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_038879231.1 guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_038879232.1 guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida])

HSP 1 Score: 377.9 bits (969), Expect = 6.0e-101
Identity = 181/217 (83.41%), Postives = 194/217 (89.40%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSVPSLPPPSPKSPPEYPDLYGKRRE AKIQTLEREIGFLE+ELKS+QSLQP
Sbjct: 1   MAARSVYSSSVPSLPPPSPKSPPEYPDLYGKRRETAKIQTLEREIGFLEDELKSIQSLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEISDF+ A+SDPLTPTCR K +RCR WKWLCGFPCF LS LCCCSC GCSVY++
Sbjct: 61  VSICCKEISDFLTANSDPLTPTCRAKHQRCRIWKWLCGFPCFKLSWLCCCSCTGCSVYVD 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           MP CCDCK CNF SC+ CCS+PKCSFFSCP  SCCRN SCR+LCC LSLPS+SCP+ C C
Sbjct: 121 MPHCCDCKPCNFPSCLRCCSMPKCSFFSCPPLSCCRNFSCRKLCC-LSLPSISCPHCCSC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKC QVPSC LCTK+CCNPCS C CF
Sbjct: 181 CECKCSCKCSFPKCSQVPSCRLCTKSCCNPCSLCCCF 216

[Close]

BLAST of MS023140.1 vs. NCBI nr
Match: XP_008450929.1 (PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Cucumis melo] >XP_008450930.1 PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Cucumis melo] >XP_008450935.1 PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Cucumis melo] >XP_016900991.1 PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Cucumis melo] >XP_016900992.1 PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Cucumis melo] >XP_016900993.1 PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Cucumis melo])

HSP 1 Score: 374.0 bits (959), Expect = 8.7e-100
Identity = 180/217 (82.95%), Postives = 192/217 (88.48%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSV SLPPPSPKSPPEYPDLYGKRREIA IQTLEREIGFLE+ELKS+QSLQP
Sbjct: 1   MAARSVYSSSVSSLPPPSPKSPPEYPDLYGKRREIANIQTLEREIGFLEDELKSLQSLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEI+DFV A+SDPLT TCR K++RCR WKWLCGFPCFNLS LCCCSC  CSVYLE
Sbjct: 61  VSICCKEINDFVTANSDPLTSTCRVKQQRCRIWKWLCGFPCFNLSWLCCCSCTECSVYLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           MPRCC CK C F SC+ CCS+PKCSFFSCP+ SCCRN SCRRLCC LSLPS+SCP+ C C
Sbjct: 121 MPRCCGCKPCKFPSCLRCCSMPKCSFFSCPTLSCCRNFSCRRLCCFLSLPSISCPHCCSC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSC CSFPKC QVPSC LCTK+CCNPCS C CF
Sbjct: 181 CECKCSCNCSFPKCSQVPSCRLCTKSCCNPCSLCCCF 217

[Close]

BLAST of MS023140.1 vs. NCBI nr
Match: XP_022933212.1 (guanine nucleotide-binding protein subunit gamma 3-like [Cucurbita moschata] >XP_022933218.1 guanine nucleotide-binding protein subunit gamma 3-like [Cucurbita moschata] >XP_022933223.1 guanine nucleotide-binding protein subunit gamma 3-like [Cucurbita moschata])

HSP 1 Score: 373.6 bits (958), Expect = 1.1e-99
Identity = 180/217 (82.95%), Postives = 191/217 (88.02%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSVPSLPPP PKSPPEYPDLYGKRREIAKI  LEREIG LE+ELKSV+SLQP
Sbjct: 1   MAARSVYSSSVPSLPPPCPKSPPEYPDLYGKRREIAKIHMLEREIGILEDELKSVESLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEISDFV  +SDPLTPTCRTK +RCRFWKWLCGFPCFNLS LCCCSC GCSV LE
Sbjct: 61  VSICCKEISDFVTVNSDPLTPTCRTKHKRCRFWKWLCGFPCFNLSWLCCCSCTGCSVSLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           +PRCC CK CNF SC +CC+ PKCSFFSCPSS CCRNISCRR CC LSLPS+SC + CCC
Sbjct: 121 VPRCCVCKPCNFPSCSNCCNKPKCSFFSCPSSFCCRNISCRRRCCTLSLPSISCSDGCCC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKC QVPSC  CTK+CC+PCS C CF
Sbjct: 181 CECKCSCKCSFPKCSQVPSCRFCTKSCCDPCSLCCCF 217

[Close]

BLAST of MS023140.1 vs. NCBI nr
Match: XP_011660020.1 (guanine nucleotide-binding protein subunit gamma 3 [Cucumis sativus] >XP_031740139.1 guanine nucleotide-binding protein subunit gamma 3 [Cucumis sativus] >XP_031740141.1 guanine nucleotide-binding protein subunit gamma 3 [Cucumis sativus] >XP_031740148.1 guanine nucleotide-binding protein subunit gamma 3 [Cucumis sativus])

HSP 1 Score: 373.6 bits (958), Expect = 1.1e-99
Identity = 179/217 (82.49%), Postives = 192/217 (88.48%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSV SLPPPSPKSPPEYPDLYGKRREIA IQTLEREIGFLE+ELKS+QSLQP
Sbjct: 1   MAARSVYSSSVSSLPPPSPKSPPEYPDLYGKRREIANIQTLEREIGFLEDELKSLQSLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEI+DFV  +SDPLTPTCR K++RCR WKWLCGFPCFNLS LCCCSC  C++YLE
Sbjct: 61  VSICCKEINDFVTTNSDPLTPTCRVKQQRCRIWKWLCGFPCFNLSWLCCCSCTECAIYLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           MPRCC CK CNF SC+ CCS+PKCSFFSCPS SCCRN SCRRLCC LSLPS+ CP+   C
Sbjct: 121 MPRCCGCKPCNFPSCLRCCSMPKCSFFSCPSLSCCRNFSCRRLCCFLSLPSIPCPHCSSC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKC QVPSC LCTK+CCNPCS C CF
Sbjct: 181 CECKCSCKCSFPKCSQVPSCRLCTKSCCNPCSLCCCF 217

[Close]

BLAST of MS023140.1 vs. ExPASy Swiss-Prot
Match: Q6AWT8 (Guanine nucleotide-binding protein subunit gamma 3 OS=Arabidopsis thaliana OX=3702 GN=GG3 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.0e-42
Identity = 118/236 (50.00%), Postives = 137/236 (58.05%), Query Frame = 0


Query: 2   ATRSVYSSSV--PSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQ 61
           A   V SSS+   SLPPP PKSPPEYPDLYGKRRE A++Q LEREIGFLE E+K ++ +Q
Sbjct: 16  AAGGVSSSSLAPSSLPPPRPKSPPEYPDLYGKRREAARVQMLEREIGFLEGEIKFIEGVQ 75

Query: 62  PVSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYL 121
           P S C KE+SDFV+A+SDPL P  R  R   RFWKWLCG PC +L S CCC  + CS +L
Sbjct: 76  PASRCIKEVSDFVVANSDPLIPAQRKSRRSFRFWKWLCG-PCLSLVSFCCCCQSKCSCHL 135

Query: 122 EMPRCCDCKQCNFQSCI-------SCCS-VPKCSFFSCPSSSCCRNISCR---RLCCVLS 181
             P+CC+C  C   SCI       SCCS +  C   SCPS SC R   C      CC+ S
Sbjct: 136 RKPKCCNCTSC---SCIGSKCCDGSCCSNICCCPRLSCPSCSCFRGCWCSCPDMSCCIPS 195

Query: 182 -LPSMSCPNRCC-------CCECKCSCK-CSFPKCPQVPSC----CLCTKTCCNPC 212
              S SC    C       CC C C  +  S   CP+V  C    C C   C NPC
Sbjct: 196 CFRSCSCTRPSCLNKKKSSCCSCNCKIRWSSCFSCPKVRLCSCCFCNCKNLCSNPC 247

[Close]

BLAST of MS023140.1 vs. ExPASy Swiss-Prot
Match: Q9BYR0 (Keratin-associated protein 4-7 OS=Homo sapiens OX=9606 GN=KRTAP4-7 PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 2.9e-05
Identity = 56/176 (31.82%), Postives = 72/176 (40.91%), Query Frame = 0


Query: 61  VSICCKEISDFVMASSDPLTPT-CRTKRERCRFWKWLCGFPCFNLSSLC---CCSCAGCS 120
           VS CC  +      S D    T CR    +    +  C  P   +SS C   CC    C 
Sbjct: 2   VSSCCGSVCSDQGCSQDLCQETCCRPSCCQTTCCRTTCYRPSCCVSSCCRPQCCQSVCCQ 61

Query: 121 VYLEMPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMS--- 180
                P CC+   C+ + CIS C  P C   SC    CC+++ C+  CC  S    S   
Sbjct: 62  PTCCRPTCCETTCCHPRCCISSCCRPSCCMSSCCKPQCCQSVCCQPTCCHPSCCISSCCR 121

Query: 181 ---CPNRCCCCECKCSCKCSFPKCPQVPSCCL--------CTKTCCNP--CSACRC 217
              C +RCC  +C C   C  P C + PSCC+        C  +CC P  C  C C
Sbjct: 122 PSCCVSRCCRPQC-CQSVCCQPTCCR-PSCCISSCCRPSCCESSCCRPSCCRPCCC 175

[Close]

BLAST of MS023140.1 vs. ExPASy TrEMBL
Match: A0A6J1D2Z2 (guanine nucleotide-binding protein subunit gamma 3 OS=Momordica charantia OX=3673 GN=LOC111016512 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.2e-123
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP
Sbjct: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE
Sbjct: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC
Sbjct: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF
Sbjct: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 217

[Close]

BLAST of MS023140.1 vs. ExPASy TrEMBL
Match: A0A1S4DYE4 (guanine nucleotide-binding protein subunit gamma 3-like OS=Cucumis melo OX=3656 GN=LOC103492372 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 4.2e-100
Identity = 180/217 (82.95%), Postives = 192/217 (88.48%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSV SLPPPSPKSPPEYPDLYGKRREIA IQTLEREIGFLE+ELKS+QSLQP
Sbjct: 1   MAARSVYSSSVSSLPPPSPKSPPEYPDLYGKRREIANIQTLEREIGFLEDELKSLQSLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEI+DFV A+SDPLT TCR K++RCR WKWLCGFPCFNLS LCCCSC  CSVYLE
Sbjct: 61  VSICCKEINDFVTANSDPLTSTCRVKQQRCRIWKWLCGFPCFNLSWLCCCSCTECSVYLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           MPRCC CK C F SC+ CCS+PKCSFFSCP+ SCCRN SCRRLCC LSLPS+SCP+ C C
Sbjct: 121 MPRCCGCKPCKFPSCLRCCSMPKCSFFSCPTLSCCRNFSCRRLCCFLSLPSISCPHCCSC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSC CSFPKC QVPSC LCTK+CCNPCS C CF
Sbjct: 181 CECKCSCNCSFPKCSQVPSCRLCTKSCCNPCSLCCCF 217

[Close]

BLAST of MS023140.1 vs. ExPASy TrEMBL
Match: A0A6J1F450 (guanine nucleotide-binding protein subunit gamma 3-like OS=Cucurbita moschata OX=3662 GN=LOC111440055 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.5e-100
Identity = 180/217 (82.95%), Postives = 191/217 (88.02%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSVPSLPPP PKSPPEYPDLYGKRREIAKI  LEREIG LE+ELKSV+SLQP
Sbjct: 1   MAARSVYSSSVPSLPPPCPKSPPEYPDLYGKRREIAKIHMLEREIGILEDELKSVESLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEISDFV  +SDPLTPTCRTK +RCRFWKWLCGFPCFNLS LCCCSC GCSV LE
Sbjct: 61  VSICCKEISDFVTVNSDPLTPTCRTKHKRCRFWKWLCGFPCFNLSWLCCCSCTGCSVSLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           +PRCC CK CNF SC +CC+ PKCSFFSCPSS CCRNISCRR CC LSLPS+SC + CCC
Sbjct: 121 VPRCCVCKPCNFPSCSNCCNKPKCSFFSCPSSFCCRNISCRRRCCTLSLPSISCSDGCCC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKC QVPSC  CTK+CC+PCS C CF
Sbjct: 181 CECKCSCKCSFPKCSQVPSCRFCTKSCCDPCSLCCCF 217

[Close]

BLAST of MS023140.1 vs. ExPASy TrEMBL
Match: A0A0A0LZK9 (G protein gamma domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G597050 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.5e-100
Identity = 179/217 (82.49%), Postives = 192/217 (88.48%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSV SLPPPSPKSPPEYPDLYGKRREIA IQTLEREIGFLE+ELKS+QSLQP
Sbjct: 1   MAARSVYSSSVSSLPPPSPKSPPEYPDLYGKRREIANIQTLEREIGFLEDELKSLQSLQP 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEI+DFV  +SDPLTPTCR K++RCR WKWLCGFPCFNLS LCCCSC  C++YLE
Sbjct: 61  VSICCKEINDFVTTNSDPLTPTCRVKQQRCRIWKWLCGFPCFNLSWLCCCSCTECAIYLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           MPRCC CK CNF SC+ CCS+PKCSFFSCPS SCCRN SCRRLCC LSLPS+ CP+   C
Sbjct: 121 MPRCCGCKPCNFPSCLRCCSMPKCSFFSCPSLSCCRNFSCRRLCCFLSLPSIPCPHCSSC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKC QVPSC LCTK+CCNPCS C CF
Sbjct: 181 CECKCSCKCSFPKCSQVPSCRLCTKSCCNPCSLCCCF 217

[Close]

BLAST of MS023140.1 vs. ExPASy TrEMBL
Match: A0A6J1HWR3 (guanine nucleotide-binding protein subunit gamma 3-like OS=Cucurbita maxima OX=3661 GN=LOC111467550 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.8e-96
Identity = 176/217 (81.11%), Postives = 188/217 (86.64%), Query Frame = 0


Query: 1   MATRSVYSSSVPSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQP 60
           MA RSVYSSSVPSLPPP PKSPPEYPDLYGKRREIAKI  LEREIG LE+ELKSV+SLQ 
Sbjct: 1   MAARSVYSSSVPSLPPPCPKSPPEYPDLYGKRREIAKIHMLEREIGILEDELKSVESLQS 60

Query: 61  VSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYLE 120
           VSICCKEISDFV  +SDPLTPTCRTK +RCRFWKWLCGFPCFNLS LCCCSC GCSV LE
Sbjct: 61  VSICCKEISDFVTVNSDPLTPTCRTKHKRCRFWKWLCGFPCFNLSWLCCCSCTGCSVSLE 120

Query: 121 MPRCCDCKQCNFQSCISCCSVPKCSFFSCPSSSCCRNISCRRLCCVLSLPSMSCPNRCCC 180
           +P CC CK CNF SC +CC+ PKCSFFSCPSS CCRNISCRR CC+LSLPS+SC + C C
Sbjct: 121 VPSCCVCKPCNFPSCSNCCNKPKCSFFSCPSSFCCRNISCRRRCCILSLPSISCSDGCRC 180

Query: 181 CECKCSCKCSFPKCPQVPSCCLCTKTCCNPCSACRCF 218
           CECKCSCKCSFPKC QVPSC  CTK+CC+PCS C  F
Sbjct: 181 CECKCSCKCSFPKCSQVPSCRFCTKSCCDPCSLCCWF 217

[Close]

BLAST of MS023140.1 vs. TAIR 10
Match: AT5G20635.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: keratin filament, chloroplast; CONTAINS InterPro DOMAIN/s: Keratin, high sulphur B2 protein (InterPro:IPR002494); Has 6153 Blast hits to 2882 proteins in 265 species: Archae - 6; Bacteria - 47; Metazoa - 4777; Fungi - 68; Plants - 187; Viruses - 30; Other Eukaryotes - 1038 (source: NCBI BLink). )

HSP 1 Score: 174.9 bits (442), Expect = 7.2e-44
Identity = 118/236 (50.00%), Postives = 137/236 (58.05%), Query Frame = 0


Query: 2   ATRSVYSSSV--PSLPPPSPKSPPEYPDLYGKRREIAKIQTLEREIGFLEEELKSVQSLQ 61
           A   V SSS+   SLPPP PKSPPEYPDLYGKRRE A++Q LEREIGFLE E+K ++ +Q
Sbjct: 16  AAGGVSSSSLAPSSLPPPRPKSPPEYPDLYGKRREAARVQMLEREIGFLEGEIKFIEGVQ 75

Query: 62  PVSICCKEISDFVMASSDPLTPTCRTKRERCRFWKWLCGFPCFNLSSLCCCSCAGCSVYL 121
           P S C KE+SDFV+A+SDPL P  R  R   RFWKWLCG PC +L S CCC  + CS +L
Sbjct: 76  PASRCIKEVSDFVVANSDPLIPAQRKSRRSFRFWKWLCG-PCLSLVSFCCCCQSKCSCHL 135

Query: 122 EMPRCCDCKQCNFQSCI-------SCCS-VPKCSFFSCPSSSCCRNISCR---RLCCVLS 181
             P+CC+C  C   SCI       SCCS +  C   SCPS SC R   C      CC+ S
Sbjct: 136 RKPKCCNCTSC---SCIGSKCCDGSCCSNICCCPRLSCPSCSCFRGCWCSCPDMSCCIPS 195

Query: 182 -LPSMSCPNRCC-------CCECKCSCK-CSFPKCPQVPSC----CLCTKTCCNPC 212
              S SC    C       CC C C  +  S   CP+V  C    C C   C NPC
Sbjct: 196 CFRSCSCTRPSCLNKKKSSCCSCNCKIRWSSCFSCPKVRLCSCCFCNCKNLCSNPC 247

[Close]

BLAST of MS023140.1 vs. TAIR 10
Match: AT3G22942.1 (G-protein gamma subunit 2 )

HSP 1 Score: 47.8 bits (112), Expect = 1.3e-05
Identity = 24/55 (43.64%), Postives = 33/55 (60.00%), Query Frame = 0


Query: 27 DLYGKRREIAKIQTLEREIGFLEEELKSVQSLQPVSICCKEISDFVMASSDPLTP 82
          D  GK R  A+++ LE+E  FLEEEL+ ++ +   S  CKE  D V +  DPL P
Sbjct: 18 DTRGKHRIQAELKRLEQEARFLEEELEQLEKMDNASASCKEFLDSVDSKPDPLLP 72

[Close]

BLAST of MS023140.1 vs. TAIR 10
Match: AT3G63420.1 (Ggamma-subunit 1 )

HSP 1 Score: 47.0 bits (110), Expect = 2.3e-05
Identity = 21/52 (40.38%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 30 GKRREIAKIQTLEREIGFLEEELKSVQSLQPVSICCKEISDFVMASSDPLTP 82
          GK R +A++  +E+E+ FLE+ELK V++   VS  C+E+   +    DPL P
Sbjct: 19 GKHRILAELARVEQEVAFLEKELKEVENTDIVSTVCEELLSVIEKGPDPLLP 70

[Close]

BLAST of MS023140.1 vs. TAIR 10
Match: AT3G63420.2 (Ggamma-subunit 1 )

HSP 1 Score: 47.0 bits (110), Expect = 2.3e-05
Identity = 21/52 (40.38%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 30 GKRREIAKIQTLEREIGFLEEELKSVQSLQPVSICCKEISDFVMASSDPLTP 82
          GK R +A++  +E+E+ FLE+ELK V++   VS  C+E+   +    DPL P
Sbjct: 19 GKHRILAELARVEQEVAFLEKELKEVENTDIVSTVCEELLSVIEKGPDPLLP 70

The following BLAST results are available for this feature:

BLAST of MS023140.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147636.1	2.5e-123	100.00	guanine nucleotide-binding protein subunit gamma 3 [Momordica charantia] >XP_022...	[more]
XP_038879225.1	6.0e-101	83.41	guanine nucleotide-binding protein subunit gamma 3-like [Benincasa hispida] >XP_...	[more]
XP_008450929.1	8.7e-100	82.95	PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Cucumis melo...	[more]
XP_022933212.1	1.1e-99	82.95	guanine nucleotide-binding protein subunit gamma 3-like [Cucurbita moschata] >XP...	[more]
XP_011660020.1	1.1e-99	82.49	guanine nucleotide-binding protein subunit gamma 3 [Cucumis sativus] >XP_0317401...	[more]

BLAST of MS023140.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AWT8	1.0e-42	50.00	Guanine nucleotide-binding protein subunit gamma 3 OS=Arabidopsis thaliana OX=37...	[more]
Q9BYR0	2.9e-05	31.82	Keratin-associated protein 4-7 OS=Homo sapiens OX=9606 GN=KRTAP4-7 PE=1 SV=2	[more]

BLAST of MS023140.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2Z2	1.2e-123	100.00	guanine nucleotide-binding protein subunit gamma 3 OS=Momordica charantia OX=367...	[more]
A0A1S4DYE4	4.2e-100	82.95	guanine nucleotide-binding protein subunit gamma 3-like OS=Cucumis melo OX=3656 ...	[more]
A0A6J1F450	5.5e-100	82.95	guanine nucleotide-binding protein subunit gamma 3-like OS=Cucurbita moschata OX...	[more]
A0A0A0LZK9	5.5e-100	82.49	G protein gamma domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G59...	[more]
A0A6J1HWR3	4.8e-96	81.11	guanine nucleotide-binding protein subunit gamma 3-like OS=Cucurbita maxima OX=3...	[more]

BLAST of MS023140.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20635.1	7.2e-44	50.00	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT3G22942.1	1.3e-05	43.64	G-protein gamma subunit 2	[more]
AT3G63420.1	2.3e-05	40.38	Ggamma-subunit 1	[more]
AT3G63420.2	2.3e-05	40.38	Ggamma-subunit 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 31..58
None	No IPR available	SMART	SM01224	G_gamma_2	coord: 34..104 e-value: 1.5E-5 score: 34.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR32378:SF10	GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA 3	coord: 1..214
None	No IPR available	PANTHER	PTHR32378	GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA 3	coord: 1..214
IPR015898	G-protein gamma-like domain	PFAM	PF00631	G-gamma	coord: 39..109 e-value: 2.6E-10 score: 40.2

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS023140	MS023140	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS023140.1-cds	MS023140.1-cds-scaffold78:435958..436318	CDS
MS023140.1-cds	MS023140.1-cds-scaffold78:436406..436450	CDS
MS023140.1-cds	MS023140.1-cds-scaffold78:436537..436581	CDS
MS023140.1-cds	MS023140.1-cds-scaffold78:436725..436777	CDS
MS023140.1-cds	MS023140.1-cds-scaffold78:437520..437666	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS023140.1	MS023140.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007186	G protein-coupled receptor signaling pathway