MS022952.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS022952.1 vs. NCBI nr
Match: XP_022151368.1 (strigolactone esterase D14-like [Momordica charantia])

HSP 1 Score: 561.6 bits (1446), Expect = 3.8e-156
Identity = 274/283 (96.82%), Postives = 279/283 (98.59%), Query Frame = 0

Query: 1   MHGGFFDCGEAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           MHGGFF+CGEAAI EALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS
Sbjct: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL
Sbjct: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAIS 180
           FTHLILLNASPRYIND+GYYGGFERPQLDGIFKQMDQNFTNWVK FAPFAVAVNDSKAIS
Sbjct: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKM 240
           EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKV+TPSTIILTRKDNIVPKSVAFYMKSKM
Sbjct: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240

Query: 241 GGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLKDKNHPARD 284
           GGHANVKILNSEGHFPQL AYPQ LKVLSQVLRLKDKNHP++D
Sbjct: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD 283

BLAST of MS022952.1 vs. NCBI nr
Match: XP_038905280.1 (strigolactone esterase D14-like [Benincasa hispida])

HSP 1 Score: 466.8 bits (1200), Expect = 1.3e-127
Identity = 227/284 (79.93%), Postives = 259/284 (91.20%), Query Frame = 0

Query: 1   MHGGFFDCGE-AAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFD 60
           M+GG F+CG  +A AEAL VNVYGNGT T+VLAHGFG DQS W Y+IPYLACFFKVVVFD
Sbjct: 1   MYGG-FNCGAGSAFAEALKVNVYGNGTETLVLAHGFGSDQSAWHYMIPYLACFFKVVVFD 60

Query: 61  SIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQ 120
            +FSPNVK ELYDE+KYSSFDGYA+DLVCVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQ
Sbjct: 61  LVFSPNVKLELYDERKYSSFDGYAKDLVCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQ 120

Query: 121 LFTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAI 180
           LF HLILLNASPRY+N EGY+GGF+RPQ+D IFKQMD NFT WVK+FAPFAV VNDSKA+
Sbjct: 121 LFHHLILLNASPRYLNAEGYFGGFKRPQVDEIFKQMDNNFTKWVKEFAPFAVRVNDSKAM 180

Query: 181 SEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSK 240
           +EFEHSLGRM PKIAL+VAK VFLSDLR+LLPKV+TP++IILT KDNIVPKSVAF+MKS 
Sbjct: 181 AEFEHSLGRMNPKIALNVAKMVFLSDLRKLLPKVRTPTSIILTEKDNIVPKSVAFFMKSN 240

Query: 241 MGGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLKDKNHPARD 284
           MGG++NVKIL SEGHFPQLTAYPQLLKVLS+VL+LK+  + +RD
Sbjct: 241 MGGNSNVKILKSEGHFPQLTAYPQLLKVLSKVLKLKEYKNYSRD 283

BLAST of MS022952.1 vs. NCBI nr
Match: KAG6596658.1 (Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028196.1 Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 460.7 bits (1184), Expect = 9.2e-126
Identity = 228/283 (80.57%), Postives = 250/283 (88.34%), Query Frame = 0

Query: 4   GFFDCG-----EAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVF 63
           G F+CG      AAIAEAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVF
Sbjct: 3   GGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVF 62

Query: 64  DSIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRP 123
           D +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRP
Sbjct: 63  DLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRP 122

Query: 124 QLFTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKA 183
           QLF HLILLNASPRY+N EGY GGFERP +D IF QMD+NFTNWVK FAPFAV++ND K 
Sbjct: 123 QLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDPKT 182

Query: 184 ISEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKS 243
           I+EFEHSLGRMKP+IALSVAK VFLSDLR+LLPKVKTP+TII TRKDNIVPK VA Y+KS
Sbjct: 183 IAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYIKS 242

Query: 244 KMGGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLKDKNHPA 282
           K+G HA VKILNS+GHFPQLTAYPQLLKVL+QVL LK KN  A
Sbjct: 243 KVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLKHKNSTA 285

BLAST of MS022952.1 vs. NCBI nr
Match: XP_022945470.1 (strigolactone esterase D14-like [Cucurbita moschata])

HSP 1 Score: 460.7 bits (1184), Expect = 9.2e-126
Identity = 227/282 (80.50%), Postives = 250/282 (88.65%), Query Frame = 0

Query: 4   GFFDCG----EAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFD 63
           G F+CG     AAIAEAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVFD
Sbjct: 3   GGFNCGGSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFD 62

Query: 64  SIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQ 123
            +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRPQ
Sbjct: 63  LVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQ 122

Query: 124 LFTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAI 183
           LF HLILLNASPRY+N EGY GGFERP +D IF QMD+NFTNWVK FAPFAV++ND K I
Sbjct: 123 LFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTI 182

Query: 184 SEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSK 243
           +E EHSLGRMKP+IALSVAK VFLSDLR+LLPKVKTP+TII TRKDNIVPK VAFY+KSK
Sbjct: 183 AELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYIKSK 242

Query: 244 MGGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLKDKNHPA 282
           +G HA VKILNS+GHFPQLTAYPQLLKVL+QVL L+ KN  A
Sbjct: 243 VGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQHKNSTA 284

BLAST of MS022952.1 vs. NCBI nr
Match: XP_008443004.1 (PREDICTED: strigolactone esterase D14-like [Cucumis melo] >KAA0043762.1 strigolactone esterase D14-like [Cucumis melo var. makuwa] >TYK25371.1 strigolactone esterase D14-like [Cucumis melo var. makuwa])

HSP 1 Score: 444.9 bits (1143), Expect = 5.2e-121
Identity = 214/275 (77.82%), Postives = 248/275 (90.18%), Query Frame = 0

Query: 1   MHGGFFDCGEAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AIAEAL VNVYGNGT T+VLAHGFGLDQ+VW Y+IPYLACFFKVVVFD 
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAIS 180
           F HLILLNASPRY+N EGY GGFE+PQ+D IFK+MD+NFT W K+F PFAV VN+SKA++
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIALSVAK VFLSDL +LLPKVKT ++IILT KDNIVPKSVAF++KS +
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLK 276
           GG++NV IL S+GHFPQLTAYPQLLKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

BLAST of MS022952.1 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.8e-61
Identity = 115/260 (44.23%), Postives = 170/260 (65.38%), Query Frame = 0

Query: 13  IAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           I EALNV V G G   + LAHGFG DQS W  ++PY    ++VV++D + + +V  + +D
Sbjct: 6   ILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVNPDYFD 65

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +Y++ D Y  DL+ +++ L ++   YVGHS+SAMIG +A+  RP+LF+ LIL+  SPR
Sbjct: 66  FNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPR 125

Query: 133 YINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAISEFEHSLGRMKPK 192
           ++NDE Y+GGFE  +++ +F  M+ N+  WV  FAP AV  +   A+ EF  +L  M+P 
Sbjct: 126 FLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPD 185

Query: 193 IALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNSE 252
           I+L V++ VF SDLR +L  V+ P+ +I T KD  VP SVA Y++S +GG   V+ L +E
Sbjct: 186 ISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTE 245

Query: 253 GHFPQLTAYPQLLKVLSQVL 273
           GH PQL+A  QL + L + L
Sbjct: 246 GHLPQLSAPAQLAQFLRRAL 265

BLAST of MS022952.1 vs. ExPASy Swiss-Prot
Match: Q10J20 (Probable esterase D14L OS=Oryza sativa subsp. japonica OX=39947 GN=D14L PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.6e-59
Identity = 115/258 (44.57%), Postives = 172/258 (66.67%), Query Frame = 0

Query: 13  IAEALNVNVYGNG-TATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELY 72
           + EA N+ V G G    +VLAHGFG DQSVW++++P+L   ++VV+FD++ +     + +
Sbjct: 4   VEEAHNLRVVGEGKRGVIVLAHGFGTDQSVWKHLVPHLVADYRVVLFDTMGAGPTNPDYF 63

Query: 73  DEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASP 132
           D  +Y++ +GYA DL+ +L+ L V   IYVGHS+SA+IG +A+  RP LF+ L+LL+ASP
Sbjct: 64  DFSRYATLEGYALDLLAILQELRVASCIYVGHSVSAVIGAIASISRPDLFSKLVLLSASP 123

Query: 133 RYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVN-DSKAISEFEHSLGRMK 192
           RY+ND  YYGGFE+  LD +F+ M  N+  W   FAP  V  + +S A+ EF  +L  ++
Sbjct: 124 RYLNDVDYYGGFEQEDLDELFEAMGSNYKAWCSGFAPLCVGGDMESVAVQEFSRTLFNIR 183

Query: 193 PKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILN 252
           P IALSVA+ +F SD+R LLP V  P  I+ + KD  VP  V+ Y+   +GG + V+++ 
Sbjct: 184 PDIALSVAQTIFQSDVRSLLPLVTVPCHIVQSTKDLAVPVVVSEYLHKHLGGDSIVEVMP 243

Query: 253 SEGHFPQLTAYPQLLKVL 269
           SEGH PQL++   ++ VL
Sbjct: 244 SEGHLPQLSSPDIVIPVL 261

BLAST of MS022952.1 vs. ExPASy Swiss-Prot
Match: J9U5U9 (Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.5e-59
Identity = 113/260 (43.46%), Postives = 170/260 (65.38%), Query Frame = 0

Query: 13  IAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           + +ALNV V G+G   +VLAHGFG DQS W  ++P+    ++VV++D + + +V  + +D
Sbjct: 5   LLDALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDYRVVLYDLVCAGSVNPDFFD 64

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
            ++Y++ D Y  DL+ +L+ L ++   YVGHS+SAMIG +A+  RP+LF+ LIL+ ASPR
Sbjct: 65  FRRYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGILASIRRPELFSKLILIGASPR 124

Query: 133 YINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAISEFEHSLGRMKPK 192
           ++NDE Y+GGFE+ +++ +F  M+ N+  WV  FAP AV  +   A+ EF  +L  M+P 
Sbjct: 125 FLNDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAVGADVPAAVREFSRTLFNMRPD 184

Query: 193 IALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNSE 252
           I L V++ VF SD+R +L  VK P  I  T +D+ VP SVA Y+K+ +GG   V  LN E
Sbjct: 185 ITLFVSRTVFNSDMRGVLGLVKVPCHIFQTARDHSVPASVATYLKNHLGGKNTVHWLNIE 244

Query: 253 GHFPQLTAYPQLLKVLSQVL 273
           GH P L+A   L + L + L
Sbjct: 245 GHLPHLSAPTLLAQELRRAL 264

BLAST of MS022952.1 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.5e-59
Identity = 113/249 (45.38%), Postives = 166/249 (66.67%), Query Frame = 0

Query: 13  IAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           I +A NV V G+G   +V AHGFG DQS W+ V+PY    +KV+++D + + +V  + +D
Sbjct: 5   ILDAFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSVNPDHFD 64

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
            ++Y++ D Y  DL+ +L+ LHV R  YVGHS+SAM G +A+  RP+LF+ LIL+ ASPR
Sbjct: 65  FRRYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGASPR 124

Query: 133 YIND-EGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAISEFEHSLGRMKP 192
           ++ND E Y+GGFE+ +++ +F  M+ N+  WV  FAP AV  +   A+ EF  +L  M+P
Sbjct: 125 FLNDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFNMRP 184

Query: 193 KIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNS 252
            I+L V++ VF SDLR +L  V  P  I+ T +D  VP SVA YMK  +GG + V+ L++
Sbjct: 185 DISLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQWLDT 244

Query: 253 EGHFPQLTA 261
           EGH P L+A
Sbjct: 245 EGHLPHLSA 253

BLAST of MS022952.1 vs. ExPASy Swiss-Prot
Match: Q9SZU7 (Probable esterase KAI2 OS=Arabidopsis thaliana OX=3702 GN=KAI2 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 7.7e-59
Identity = 110/262 (41.98%), Postives = 178/262 (67.94%), Query Frame = 0

Query: 13  IAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           + EA NV V G+G AT+VL HGFG DQSVW++++P+L   ++VV++D++ +     + +D
Sbjct: 4   VEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFD 63

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +YS+ +GY+ DL+ +LE L +E  I+VGHS+SAMIG +A+  RP LF+ +++++ASPR
Sbjct: 64  FDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPR 123

Query: 133 YINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVN-DSKAISEFEHSLGRMKP 192
           Y+ND  Y GGFE+  L+ +F+ +  N+  W   FAP AV  + DS A+ EF  +L  M+P
Sbjct: 124 YVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRP 183

Query: 193 KIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNS 252
            IALSV + +F SD+R++LP V  P  I+ + KD  VP  V+ Y+ + +G  + V+++ S
Sbjct: 184 DIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPS 243

Query: 253 EGHFPQLTAYPQLLKVLSQVLR 274
           +GH PQL++   ++ V+ + +R
Sbjct: 244 DGHLPQLSSPDSVIPVILRHIR 265

BLAST of MS022952.1 vs. ExPASy TrEMBL
Match: A0A6J1DC02 (strigolactone esterase D14-like OS=Momordica charantia OX=3673 GN=LOC111019326 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.9e-156
Identity = 274/283 (96.82%), Postives = 279/283 (98.59%), Query Frame = 0

Query: 1   MHGGFFDCGEAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           MHGGFF+CGEAAI EALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS
Sbjct: 1   MHGGFFNCGEAAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL
Sbjct: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAIS 180
           FTHLILLNASPRYIND+GYYGGFERPQLDGIFKQMDQNFTNWVK FAPFAVAVNDSKAIS
Sbjct: 121 FTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAIS 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKM 240
           EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKV+TPSTIILTRKDNIVPKSVAFYMKSKM
Sbjct: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSKM 240

Query: 241 GGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLKDKNHPARD 284
           GGHANVKILNSEGHFPQL AYPQ LKVLSQVLRLKDKNHP++D
Sbjct: 241 GGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLKDKNHPSQD 283

BLAST of MS022952.1 vs. ExPASy TrEMBL
Match: A0A6J1G109 (strigolactone esterase D14-like OS=Cucurbita moschata OX=3662 GN=LOC111449693 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.5e-126
Identity = 227/282 (80.50%), Postives = 250/282 (88.65%), Query Frame = 0

Query: 4   GFFDCG----EAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFD 63
           G F+CG     AAIAEAL VNVYG G  T+VLAHGFGLDQS WRY+IPYLACFFKVVVFD
Sbjct: 3   GGFNCGGSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVVFD 62

Query: 64  SIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQ 123
            +FSPNVK E+YDE+KYSSF+GYA+DLVCVL+HLHVERSIYVGHSMSAMIGCVAATYRPQ
Sbjct: 63  LVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYRPQ 122

Query: 124 LFTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAI 183
           LF HLILLNASPRY+N EGY GGFERP +D IF QMD+NFTNWVK FAPFAV++ND K I
Sbjct: 123 LFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDPKTI 182

Query: 184 SEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSK 243
           +E EHSLGRMKP+IALSVAK VFLSDLR+LLPKVKTP+TII TRKDNIVPK VAFY+KSK
Sbjct: 183 AELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYIKSK 242

Query: 244 MGGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLKDKNHPA 282
           +G HA VKILNS+GHFPQLTAYPQLLKVL+QVL L+ KN  A
Sbjct: 243 VGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQHKNSTA 284

BLAST of MS022952.1 vs. ExPASy TrEMBL
Match: A0A5A7TKE6 (Strigolactone esterase D14-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005110 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.5e-121
Identity = 214/275 (77.82%), Postives = 248/275 (90.18%), Query Frame = 0

Query: 1   MHGGFFDCGEAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AIAEAL VNVYGNGT T+VLAHGFGLDQ+VW Y+IPYLACFFKVVVFD 
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAIS 180
           F HLILLNASPRY+N EGY GGFE+PQ+D IFK+MD+NFT W K+F PFAV VN+SKA++
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIALSVAK VFLSDL +LLPKVKT ++IILT KDNIVPKSVAF++KS +
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLK 276
           GG++NV IL S+GHFPQLTAYPQLLKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

BLAST of MS022952.1 vs. ExPASy TrEMBL
Match: A0A1S3B719 (strigolactone esterase D14-like OS=Cucumis melo OX=3656 GN=LOC103486734 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.5e-121
Identity = 214/275 (77.82%), Postives = 248/275 (90.18%), Query Frame = 0

Query: 1   MHGGFFDCGEAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AIAEAL VNVYGNGT T+VLAHGFGLDQ+VW Y+IPYLACFFKVVVFD 
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAIS 180
           F HLILLNASPRY+N EGY GGFE+PQ+D IFK+MD+NFT W K+F PFAV VN+SKA++
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIALSVAK VFLSDL +LLPKVKT ++IILT KDNIVPKSVAF++KS +
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLK 276
           GG++NV IL S+GHFPQLTAYPQLLKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLK 275

BLAST of MS022952.1 vs. ExPASy TrEMBL
Match: A0A0A0LB72 (Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_3G792050 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.8e-120
Identity = 209/275 (76.00%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MHGGFFDCGEAAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDS 60
           M+ GF   G +AIAEALNVNVYGNG+ T+VLAHGFGLDQ+VW Y++PYLACFFKVVVFD 
Sbjct: 1   MYNGFNCGGGSAIAEALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60

Query: 61  IFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQL 120
           +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FTHLILLNASPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAIS 180
           F HL+LLNASPRY+N EGY GGFE+PQ+D + K+MD+NFT W K+FAPFAV VN+SKA++
Sbjct: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180

Query: 181 EFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKM 240
           E EHSLGRM PKIAL+VAK VFLSDL +LLPKVKT ++IILT+KDNIVPKSVAF++KS +
Sbjct: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240

Query: 241 GGHANVKILNSEGHFPQLTAYPQLLKVLSQVLRLK 276
           GG++NV IL S+GHFPQLTA+PQLLKVL++VL+LK
Sbjct: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLK 275

BLAST of MS022952.1 vs. TAIR 10
Match: AT3G03990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 3.4e-62
Identity = 115/260 (44.23%), Postives = 170/260 (65.38%), Query Frame = 0

Query: 13  IAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           I EALNV V G G   + LAHGFG DQS W  ++PY    ++VV++D + + +V  + +D
Sbjct: 6   ILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVNPDYFD 65

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +Y++ D Y  DL+ +++ L ++   YVGHS+SAMIG +A+  RP+LF+ LIL+  SPR
Sbjct: 66  FNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPR 125

Query: 133 YINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAISEFEHSLGRMKPK 192
           ++NDE Y+GGFE  +++ +F  M+ N+  WV  FAP AV  +   A+ EF  +L  M+P 
Sbjct: 126 FLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPD 185

Query: 193 IALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNSE 252
           I+L V++ VF SDLR +L  V+ P+ +I T KD  VP SVA Y++S +GG   V+ L +E
Sbjct: 186 ISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTE 245

Query: 253 GHFPQLTAYPQLLKVLSQVL 273
           GH PQL+A  QL + L + L
Sbjct: 246 GHLPQLSAPAQLAQFLRRAL 265

BLAST of MS022952.1 vs. TAIR 10
Match: AT3G24420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-61
Identity = 120/264 (45.45%), Postives = 176/264 (66.67%), Query Frame = 0

Query: 11  AAIAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHE- 70
           + +A A+N  + G+G  ++VLAHGFG DQSVW  +IP L+  FKV+VFD +FS  +K + 
Sbjct: 8   SGLASAMNAKIIGSGERSMVLAHGFGGDQSVWDKIIPVLSQSFKVLVFDWLFSGAIKDQT 67

Query: 71  LYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNA 130
           LYD  KY+S D ++ DL+ ++E L     ++VGHSMS +IGC A+  RP LFT+L+L+ A
Sbjct: 68  LYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCAASIKRPDLFTNLLLIAA 127

Query: 131 SPRYINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVNDSKAISEFEHSLGRM 190
           SPRYIN E Y GGFE   +D I   +  N+  W   F+ F V   DS ++  FE SL +M
Sbjct: 128 SPRYINSEDYKGGFESKDIDTIITSIGSNYEAWAVDFSSFVVDSRDSLSVQRFEKSLKKM 187

Query: 191 KPKIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKIL 250
           KP+ AL++AK VF SD R +L +V  P  +I    D +VP SVA++M+ K+ G + V+I+
Sbjct: 188 KPETALALAKIVFGSDEREILGQVSVPCHVIQPGNDVVVPVSVAYFMQEKIKGKSTVEII 247

Query: 251 -NSEGHFPQLTAYPQLLKVLSQVL 273
            ++ GHFPQ+T++ +LL V+ ++L
Sbjct: 248 EDAIGHFPQMTSHLELLGVMRRLL 271

BLAST of MS022952.1 vs. TAIR 10
Match: AT4G37470.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 5.5e-60
Identity = 110/262 (41.98%), Postives = 178/262 (67.94%), Query Frame = 0

Query: 13  IAEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFDSIFSPNVKHELYD 72
           + EA NV V G+G AT+VL HGFG DQSVW++++P+L   ++VV++D++ +     + +D
Sbjct: 4   VEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFD 63

Query: 73  EKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPR 132
             +YS+ +GY+ DL+ +LE L +E  I+VGHS+SAMIG +A+  RP LF+ +++++ASPR
Sbjct: 64  FDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPR 123

Query: 133 YINDEGYYGGFERPQLDGIFKQMDQNFTNWVKKFAPFAVAVN-DSKAISEFEHSLGRMKP 192
           Y+ND  Y GGFE+  L+ +F+ +  N+  W   FAP AV  + DS A+ EF  +L  M+P
Sbjct: 124 YVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRP 183

Query: 193 KIALSVAKKVFLSDLRRLLPKVKTPSTIILTRKDNIVPKSVAFYMKSKMGGHANVKILNS 252
            IALSV + +F SD+R++LP V  P  I+ + KD  VP  V+ Y+ + +G  + V+++ S
Sbjct: 184 DIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPS 243

Query: 253 EGHFPQLTAYPQLLKVLSQVLR 274
           +GH PQL++   ++ V+ + +R
Sbjct: 244 DGHLPQLSSPDSVIPVILRHIR 265

BLAST of MS022952.1 vs. TAIR 10
Match: AT5G53050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.5e-04
Identity = 26/68 (38.24%), Postives = 36/68 (52.94%), Query Frame = 0

Query: 83  ARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPRYINDEGYYGG 142
           A D + +L+HL  +++  +GHSM AMI C  A   P+    L LLN +          GG
Sbjct: 107 ANDSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTG---------GG 165

Query: 143 FE-RPQLD 150
           FE  P+LD
Sbjct: 167 FECFPKLD 165

BLAST of MS022952.1 vs. TAIR 10
Match: AT5G53050.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.5e-04
Identity = 26/68 (38.24%), Postives = 36/68 (52.94%), Query Frame = 0

Query: 83  ARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQLFTHLILLNASPRYINDEGYYGG 142
           A D + +L+HL  +++  +GHSM AMI C  A   P+    L LLN +          GG
Sbjct: 107 ANDSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTG---------GG 165

Query: 143 FE-RPQLD 150
           FE  P+LD
Sbjct: 167 FECFPKLD 165