MS021655.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS021655.1 vs. NCBI nr
Match: XP_022137292.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111008789 [Momordica charantia])

HSP 1 Score: 481.5 bits (1238), Expect = 4.2e-132
Identity = 232/236 (98.31%), Postives = 234/236 (99.15%), Query Frame = 0

Query: 1   PLLHLSLASMLLILLISLPASSAYQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE 60
           PLLHLSLASMLLILLISLPASSAY+EWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE
Sbjct: 733 PLLHLSLASMLLILLISLPASSAYEEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE 792

Query: 61  AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAII 120
           AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSI IYAII
Sbjct: 793 AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSIPIYAII 852

Query: 121 PVSYECGNPTCFGTKFSYIPFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQNIR 180
           PVSYEC NPTCFGTKFSY+PFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQNIR
Sbjct: 853 PVSYECSNPTCFGTKFSYVPFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQNIR 912

Query: 181 AEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAASP 237
           AEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAASP
Sbjct: 913 AEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAASP 968

BLAST of MS021655.1 vs. NCBI nr
Match: XP_022137109.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.3 isoform X1 [Momordica charantia])

HSP 1 Score: 271.2 bits (692), Expect = 8.6e-69
Identity = 144/243 (59.26%), Postives = 173/243 (71.19%), Query Frame = 0

Query: 3   LHLSLASMLLILLISLPASSA-YQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPEA 62
           LHLSLA MLL+LL S PASSA Y EWFLNC D L CES+GT+EFPLWR + TG+C  PE 
Sbjct: 11  LHLSLAFMLLMLLNSSPASSAFYGEWFLNCNDPLKCESIGTLEFPLWRNNRTGTCSYPET 70

Query: 63  MKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAIIP 122
           MK+NC+GSR TT +I GA++ELL F T DQIL++ EIDFSD FC    KN S  IY IIP
Sbjct: 71  MKVNCDGSRGTTIEILGAEYELLSFNTNDQILIIGEIDFSDGFC---SKNISSYIYGIIP 130

Query: 123 VSYECGNP---TCFGTKFSYIPFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQN 182
           + Y C  P   TC  T+FSY+PF+N TA ADAYC +SA+V ISP + L EL  LQ V + 
Sbjct: 131 LYYYCQIPDKSTCSVTQFSYMPFQNITAIADAYCMMSAIVLISP-MWLNELGGLQNVARR 190

Query: 183 IRAEFLYEPKVDAQVCRSCSVPGGVCGYDLLLN-------QTRCYCSSPSNGFEVCSSSL 235
           IR +F  + KVDAQ CRSCSV GG+ GY+  +N       Q +C+C S SNGFE+CSSS 
Sbjct: 191 IREQFDSDLKVDAQACRSCSVSGGIFGYEDSINYNPFFFSQAKCFCKSSSNGFELCSSSR 249

BLAST of MS021655.1 vs. NCBI nr
Match: XP_022133890.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 isoform X2 [Momordica charantia])

HSP 1 Score: 235.3 bits (599), Expect = 5.2e-58
Identity = 133/236 (56.36%), Postives = 165/236 (69.92%), Query Frame = 0

Query: 1   PLLHLSLASMLLILLISLPASSAYQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE 60
           PL HL L+  LL++L++  ASS Y EWFLNC +  NCE   ++E PL R +GTG CG   
Sbjct: 9   PLFHL-LSPFLLLVLLNSAASSDYLEWFLNC-ERPNCEGNISLEIPLLRNNGTGFCGYAG 68

Query: 61  AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAII 120
            +KL CE +   T +I+GAK++LLGF+ KDQIL++AE+ FS +FC  D KNS I   A+I
Sbjct: 69  PIKLYCE-ANYATIEIWGAKYQLLGFSQKDQILIIAELGFSSRFCMPD-KNSPIQ--AMI 128

Query: 121 PVSYECGN------PTCFGTKFSYIPFENTTANADAYCKVSAVVPISPSLLLTELADLQK 180
           PVS +C +      PTC G +  YIPFEN  ANAD +C  SAVVPIS S  L EL DL+K
Sbjct: 129 PVSSDCEHPEIQRKPTCPGAELRYIPFENIAANADPHCVFSAVVPISSS-SLKELDDLKK 188

Query: 181 VEQNIRAEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSS 231
           V Q+I+A+F  + KVDAQVCRSCSVP GVCGY L  +QTRCYC +PS GFEVC SS
Sbjct: 189 VAQDIKAKFHSKLKVDAQVCRSCSVPVGVCGYGLPSDQTRCYCHAPSTGFEVCPSS 237

BLAST of MS021655.1 vs. NCBI nr
Match: XP_038885124.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 isoform X1 [Benincasa hispida])

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-49
Identity = 124/246 (50.41%), Postives = 155/246 (63.01%), Query Frame = 0

Query: 2   LLHLSLASMLLILLISLPASSAYQE--WFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRP 61
           LL L L   LL++L     +SAY E  W+LNCKD   CES+    FPLW  +G G+C  P
Sbjct: 12  LLGLYLPFFLLLIL-----ASAYGEYDWYLNCKDPPKCESIPPNGFPLWINNGAGNCYYP 71

Query: 62  EAMKLNCEGS-RRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYA 121
           E MK++CE    RTT +I+G  ++LLGF+  DQIL++AEIDFS  FC      SS SI+A
Sbjct: 72  EFMKVDCEREPARTTIEIWGENYQLLGFSMYDQILIIAEIDFSKGFCSPKRNISSKSIHA 131

Query: 122 IIPVSYECGNP------TCFGTKFSYIPFENTTANADAYCKVSAVVPISPSLLLTELADL 181
            IP + +C  P       C  T F+ IP EN    ADAYC +S +VPI+P  LL  L +L
Sbjct: 132 FIP-AVDCREPQIPDKHICSITDFAGIPSENKNLKADAYC-ISLLVPIAP-WLLDHLGNL 191

Query: 182 QKVEQNIRAEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSS--PSNGFEVCSSS 237
             V Q+I+AEF  + KVD ++CRSC+V G VCGYDLL NQT CYC S  PS  FEVCSSS
Sbjct: 192 STVAQHIKAEFNSKSKVDTEICRSCNVHGSVCGYDLLSNQTSCYCQSQLPSKRFEVCSSS 249

BLAST of MS021655.1 vs. NCBI nr
Match: XP_016899459.1 (PREDICTED: LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 isoform X1 [Cucumis melo])

HSP 1 Score: 202.2 bits (513), Expect = 4.9e-48
Identity = 118/242 (48.76%), Postives = 150/242 (61.98%), Query Frame = 0

Query: 4   HLSLASMLLILLISLPA-SSAYQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGR-PEA 63
           H   + + +ILL+S PA SS Y EWFLNC+DS  C+      FPLWRY+GT  CG   E+
Sbjct: 15  HFFPSFLFIILLLSSPAFSSLYDEWFLNCQDSFKCDGFLDKGFPLWRYNGTEFCGAYHES 74

Query: 64  MKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAIIP 123
           M++ C+G  R+T +I GA FELLG    DQIL++AEI FSD FC  +   SS S+YAIIP
Sbjct: 75  MRIKCDGI-RSTIKIAGASFELLGLGISDQILIIAEIGFSDGFCSPEKNISSSSMYAIIP 134

Query: 124 VSYECG----NPTCFGTKFSYIPFENTTA--NADAYCKVSAVVPISPSLLLTELADLQKV 183
           VSY+CG     PTC G K+ Y PFEN T   + + YC +SAVVPIS  LL     D + V
Sbjct: 135 VSYDCGLLDKKPTCSGIKYRYTPFENLTGPNSNERYCWLSAVVPISRWLLKQVGDDFRLV 194

Query: 184 EQNIRAEFLY-EPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAA 237
            Q+I  EF      VD++VC +C+ PG V  YD  L+QTRC C S  +  + C+SS    
Sbjct: 195 AQHIIEEFKSGRAVVDSRVCNNCN-PGSVYVYDFQLSQTRCCCQSSFDRIKFCASSSFTQ 254

BLAST of MS021655.1 vs. ExPASy TrEMBL
Match: A0A6J1C7V1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111008789 OS=Momordica charantia OX=3673 GN=LOC111008789 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.0e-132
Identity = 232/236 (98.31%), Postives = 234/236 (99.15%), Query Frame = 0

Query: 1   PLLHLSLASMLLILLISLPASSAYQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE 60
           PLLHLSLASMLLILLISLPASSAY+EWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE
Sbjct: 733 PLLHLSLASMLLILLISLPASSAYEEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE 792

Query: 61  AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAII 120
           AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSI IYAII
Sbjct: 793 AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSIPIYAII 852

Query: 121 PVSYECGNPTCFGTKFSYIPFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQNIR 180
           PVSYEC NPTCFGTKFSY+PFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQNIR
Sbjct: 853 PVSYECSNPTCFGTKFSYVPFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQNIR 912

Query: 181 AEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAASP 237
           AEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAASP
Sbjct: 913 AEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAASP 968

BLAST of MS021655.1 vs. ExPASy TrEMBL
Match: A0A6J1C6A5 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008666 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 4.2e-69
Identity = 144/243 (59.26%), Postives = 173/243 (71.19%), Query Frame = 0

Query: 3   LHLSLASMLLILLISLPASSA-YQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPEA 62
           LHLSLA MLL+LL S PASSA Y EWFLNC D L CES+GT+EFPLWR + TG+C  PE 
Sbjct: 11  LHLSLAFMLLMLLNSSPASSAFYGEWFLNCNDPLKCESIGTLEFPLWRNNRTGTCSYPET 70

Query: 63  MKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAIIP 122
           MK+NC+GSR TT +I GA++ELL F T DQIL++ EIDFSD FC    KN S  IY IIP
Sbjct: 71  MKVNCDGSRGTTIEILGAEYELLSFNTNDQILIIGEIDFSDGFC---SKNISSYIYGIIP 130

Query: 123 VSYECGNP---TCFGTKFSYIPFENTTANADAYCKVSAVVPISPSLLLTELADLQKVEQN 182
           + Y C  P   TC  T+FSY+PF+N TA ADAYC +SA+V ISP + L EL  LQ V + 
Sbjct: 131 LYYYCQIPDKSTCSVTQFSYMPFQNITAIADAYCMMSAIVLISP-MWLNELGGLQNVARR 190

Query: 183 IRAEFLYEPKVDAQVCRSCSVPGGVCGYDLLLN-------QTRCYCSSPSNGFEVCSSSL 235
           IR +F  + KVDAQ CRSCSV GG+ GY+  +N       Q +C+C S SNGFE+CSSS 
Sbjct: 191 IREQFDSDLKVDAQACRSCSVSGGIFGYEDSINYNPFFFSQAKCFCKSSSNGFELCSSSR 249

BLAST of MS021655.1 vs. ExPASy TrEMBL
Match: A0A6J1BWI2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111006329 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 2.5e-58
Identity = 133/236 (56.36%), Postives = 165/236 (69.92%), Query Frame = 0

Query: 1   PLLHLSLASMLLILLISLPASSAYQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGRPE 60
           PL HL L+  LL++L++  ASS Y EWFLNC +  NCE   ++E PL R +GTG CG   
Sbjct: 9   PLFHL-LSPFLLLVLLNSAASSDYLEWFLNC-ERPNCEGNISLEIPLLRNNGTGFCGYAG 68

Query: 61  AMKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAII 120
            +KL CE +   T +I+GAK++LLGF+ KDQIL++AE+ FS +FC  D KNS I   A+I
Sbjct: 69  PIKLYCE-ANYATIEIWGAKYQLLGFSQKDQILIIAELGFSSRFCMPD-KNSPIQ--AMI 128

Query: 121 PVSYECGN------PTCFGTKFSYIPFENTTANADAYCKVSAVVPISPSLLLTELADLQK 180
           PVS +C +      PTC G +  YIPFEN  ANAD +C  SAVVPIS S  L EL DL+K
Sbjct: 129 PVSSDCEHPEIQRKPTCPGAELRYIPFENIAANADPHCVFSAVVPISSS-SLKELDDLKK 188

Query: 181 VEQNIRAEFLYEPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSS 231
           V Q+I+A+F  + KVDAQVCRSCSVP GVCGY L  +QTRCYC +PS GFEVC SS
Sbjct: 189 VAQDIKAKFHSKLKVDAQVCRSCSVPVGVCGYGLPSDQTRCYCHAPSTGFEVCPSS 237

BLAST of MS021655.1 vs. ExPASy TrEMBL
Match: A0A5D3C454 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001750 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 2.4e-48
Identity = 118/242 (48.76%), Postives = 150/242 (61.98%), Query Frame = 0

Query: 4   HLSLASMLLILLISLPA-SSAYQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGR-PEA 63
           H   + + +ILL+S PA SS Y EWFLNC+DS  C+      FPLWRY+GT  CG   E+
Sbjct: 15  HFFPSFLFIILLLSSPAFSSLYDEWFLNCQDSFKCDGFLDKGFPLWRYNGTEFCGAYHES 74

Query: 64  MKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAIIP 123
           M++ C+G  R+T +I GA FELLG    DQIL++AEI FSD FC  +   SS S+YAIIP
Sbjct: 75  MRIKCDGI-RSTIKIAGASFELLGLGISDQILIIAEIGFSDGFCSPEKNISSSSMYAIIP 134

Query: 124 VSYECG----NPTCFGTKFSYIPFENTTA--NADAYCKVSAVVPISPSLLLTELADLQKV 183
           VSY+CG     PTC G K+ Y PFEN T   + + YC +SAVVPIS  LL     D + V
Sbjct: 135 VSYDCGLLDKKPTCSGIKYRYTPFENLTGPNSNERYCWLSAVVPISRWLLKQVGDDFRLV 194

Query: 184 EQNIRAEFLY-EPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAA 237
            Q+I  EF      VD++VC +C+ PG V  YD  L+QTRC C S  +  + C+SS    
Sbjct: 195 AQHIIEEFKSGRAVVDSRVCNNCN-PGSVYVYDFQLSQTRCCCQSSFDRIKFCASSSFTQ 254

BLAST of MS021655.1 vs. ExPASy TrEMBL
Match: A0A1S4DTZ4 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485709 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 2.4e-48
Identity = 118/242 (48.76%), Postives = 150/242 (61.98%), Query Frame = 0

Query: 4   HLSLASMLLILLISLPA-SSAYQEWFLNCKDSLNCESVGTIEFPLWRYDGTGSCGR-PEA 63
           H   + + +ILL+S PA SS Y EWFLNC+DS  C+      FPLWRY+GT  CG   E+
Sbjct: 15  HFFPSFLFIILLLSSPAFSSLYDEWFLNCQDSFKCDGFLDKGFPLWRYNGTEFCGAYHES 74

Query: 64  MKLNCEGSRRTTTQIFGAKFELLGFTTKDQILMVAEIDFSDQFCWGDHKNSSISIYAIIP 123
           M++ C+G  R+T +I GA FELLG    DQIL++AEI FSD FC  +   SS S+YAIIP
Sbjct: 75  MRIKCDGI-RSTIKIAGASFELLGLGISDQILIIAEIGFSDGFCSPEKNISSSSMYAIIP 134

Query: 124 VSYECG----NPTCFGTKFSYIPFENTTA--NADAYCKVSAVVPISPSLLLTELADLQKV 183
           VSY+CG     PTC G K+ Y PFEN T   + + YC +SAVVPIS  LL     D + V
Sbjct: 135 VSYDCGLLDKKPTCSGIKYRYTPFENLTGPNSNERYCWLSAVVPISRWLLKQVGDDFRLV 194

Query: 184 EQNIRAEFLY-EPKVDAQVCRSCSVPGGVCGYDLLLNQTRCYCSSPSNGFEVCSSSLIAA 237
            Q+I  EF      VD++VC +C+ PG V  YD  L+QTRC C S  +  + C+SS    
Sbjct: 195 AQHIIEEFKSGRAVVDSRVCNNCN-PGSVYVYDFQLSQTRCCCQSSFDRIKFCASSSFTQ 254