MS021645.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS021645.1 vs. NCBI nr
Match: XP_022137041.1 (synaptotagmin-5-like [Momordica charantia])

HSP 1 Score: 1660.6 bits (4299), Expect = 0.0e+00
Identity = 835/899 (92.88%), Postives = 835/899 (92.88%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL
Sbjct: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGE         
Sbjct: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420
           YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420

Query: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480
           GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP
Sbjct: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480

Query: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540
           DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY
Sbjct: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540

Query: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600
           GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK
Sbjct: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600

Query: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660
           FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ
Sbjct: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660

Query: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720
           IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV
Sbjct: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720

Query: 721 MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780
           MYKTLSPQWNQVLEFPDN SPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL
Sbjct: 721 MYKTLSPQWNQVLEFPDNSSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780

Query: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 840
           QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED
Sbjct: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 836

Query: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
           CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY
Sbjct: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 836

BLAST of MS021645.1 vs. NCBI nr
Match: XP_038894221.1 (uncharacterized protein PYUK71.03c-like [Benincasa hispida])

HSP 1 Score: 1550.4 bits (4013), Expect = 0.0e+00
Identity = 772/899 (85.87%), Postives = 799/899 (88.88%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           M+R  GP GRKRGFFNGEG MEFFHHLMAEKP LPFLIPL LIAW+IERWVFS SNWVPL
Sbjct: 1   MTRDGGPRGRKRGFFNGEGVMEFFHHLMAEKPFLPFLIPLVLIAWSIERWVFSLSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AV VWATLQYGSYQRQLIVDDLN +WRRLITNTSPETPLEPCAWLNK+L+EVWPNYFNPK
Sbjct: 61  AVVVWATLQYGSYQRQLIVDDLNTKWRRLITNTSPETPLEPCAWLNKMLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTKF+STVNKRLKDRKSRLIEKIELL+FSLGSCPPSLGLSGTRWSTCG E         
Sbjct: 121 LSTKFTSTVNKRLKDRKSRLIEKIELLDFSLGSCPPSLGLSGTRWSTCGDE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKLAKP MGTARIVINSLHIKGDLVLMPILDG+A+LFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLAKPLMGTARIVINSLHIKGDLVLMPILDGRAILFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRC SLP VDLRKKAVGGI+
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCFSLPAVDLRKKAVGGIV 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420
           YVTVISARKLYRSSLKGSPTRRQ S S NGSFGEHLTDKD+QTFVEVELEKLSRKT+ARS
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQQSYSANGSFGEHLTDKDLQTFVEVELEKLSRKTDARS 420

Query: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480
           GSDPQWNTTFNMILHED GTLRFHLYEYNPSHVKHDYLASCEVKMKY ADDSTTFWAIGP
Sbjct: 421 GSDPQWNTTFNMILHEDIGTLRFHLYEYNPSHVKHDYLASCEVKMKYAADDSTTFWAIGP 480

Query: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540
           DSRV+A HADFCGKEVEM IPFEGAHCGEL VRLVLKEWMFSDGSHSSN YHV PQQSLY
Sbjct: 481 DSRVVAKHADFCGKEVEMDIPFEGAHCGELKVRLVLKEWMFSDGSHSSNRYHVSPQQSLY 540

Query: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600
           G SN ++STGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNP WNQK
Sbjct: 541 GASNLVSSTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKALQRTRTAHSFNPTWNQK 600

Query: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660
           FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGS+RDVWIPLEKVNSGELRLQ
Sbjct: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSVRDVWIPLEKVNSGELRLQ 660

Query: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720
           IEAIRVDDNEGS+GSSL P NGWIELVL+EA+DLVAADLRGTSDPYVRVQYGKLKKRTKV
Sbjct: 661 IEAIRVDDNEGSRGSSLAPTNGWIELVLLEARDLVAADLRGTSDPYVRVQYGKLKKRTKV 720

Query: 721 MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780
           MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIPL
Sbjct: 721 MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIPL 780

Query: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 840
           QGVKRGEIHIQIT+RVPELDKRSSLDSR SLDSE+P N+A +ISSQMKQMM K Q+FIED
Sbjct: 781 QGVKRGEIHIQITKRVPELDKRSSLDSRTSLDSEIPMNKAHQISSQMKQMMNKLQTFIED 836

Query: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
            N+EGL TA+SELESLEDLQEEYMVQLETEQMLLINKIKELGQEFL+SSPSLSRRSSGY
Sbjct: 841 SNVEGLTTAMSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLNSSPSLSRRSSGY 836

BLAST of MS021645.1 vs. NCBI nr
Match: XP_004137475.2 (synaptotagmin-5 isoform X1 [Cucumis sativus] >KGN64121.1 hypothetical protein Csa_013770 [Cucumis sativus])

HSP 1 Score: 1545.0 bits (3999), Expect = 0.0e+00
Identity = 773/900 (85.89%), Postives = 800/900 (88.89%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           M+R  GP GRKRGFFNGEG MEFFHHLMAEKPLLPFLIPL LIAW+IERWVFS SNWVPL
Sbjct: 1   MARDGGPRGRKRGFFNGEGVMEFFHHLMAEKPLLPFLIPLVLIAWSIERWVFSLSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQRQLIVD+LN +WRR+ITNTSPETPLEPCAWLNKLL+EVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRQLIVDELNTKWRRIITNTSPETPLEPCAWLNKLLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTKF+STVNKRLKDRKSRLIEKIELL+FSLGSCPPSLGLSGTRWSTCG E         
Sbjct: 121 LSTKFTSTVNKRLKDRKSRLIEKIELLDFSLGSCPPSLGLSGTRWSTCGDE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKL KPFMGTARIVINSLHIKGDLVLMPILDG+AVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLGKPFMGTARIVINSLHIKGDLVLMPILDGRAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRC SLP VDLRKKAVGGI+
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCFSLPAVDLRKKAVGGIV 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCS-TNGSFGEHLTDKDMQTFVEVELEKLSRKTEAR 420
           YVTVISARKLYRSSLKGSPTRRQ S S  NGSFGEHLTDKDMQTFVEVELEKLSRKT+AR
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQQSYSANNGSFGEHLTDKDMQTFVEVELEKLSRKTDAR 420

Query: 421 SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIG 480
           SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKY ADDSTTFWAIG
Sbjct: 421 SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYAADDSTTFWAIG 480

Query: 481 PDSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSL 540
           PDS V+A +ADFCGKEVEM IPFEGAHCGEL VRLVLKEWMFSDGSHSSN YHV  QQSL
Sbjct: 481 PDSSVVAKYADFCGKEVEMDIPFEGAHCGELRVRLVLKEWMFSDGSHSSNRYHVSSQQSL 540

Query: 541 YGPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQ 600
           YG S+FL+STGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNP WNQ
Sbjct: 541 YGASSFLSSTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKALQRTRTAHSFNPTWNQ 600

Query: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRL 660
           KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGS+RDVWIPLEKVNSGELRL
Sbjct: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSVRDVWIPLEKVNSGELRL 660

Query: 661 QIEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTK 720
           QIEAIRVDDNEGSKGSSL P NGWIELVLIEA+DLVAAD+RGTSDPYVRVQYGKLKKRTK
Sbjct: 661 QIEAIRVDDNEGSKGSSLAPTNGWIELVLIEARDLVAADIRGTSDPYVRVQYGKLKKRTK 720

Query: 721 VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIP 780
           +MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIP
Sbjct: 721 IMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIP 780

Query: 781 LQGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIE 840
           LQGVKRGEIHIQIT+RVPELDKRSSLDS+ SLDSE P N+A ++SSQMKQMM K Q+FIE
Sbjct: 781 LQGVKRGEIHIQITKRVPELDKRSSLDSKTSLDSEFPMNKAHQVSSQMKQMMNKLQTFIE 837

Query: 841 DCNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
           D NLEGLATA+SELESLEDLQEEYMVQLE EQMLLINKIKELGQEFL+SSPSLSRRSSGY
Sbjct: 841 DSNLEGLATAMSELESLEDLQEEYMVQLENEQMLLINKIKELGQEFLNSSPSLSRRSSGY 837

BLAST of MS021645.1 vs. NCBI nr
Match: XP_008458181.1 (PREDICTED: synaptotagmin-5-like [Cucumis melo])

HSP 1 Score: 1543.9 bits (3996), Expect = 0.0e+00
Identity = 774/900 (86.00%), Postives = 800/900 (88.89%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           M+R  GP GRKRGFFNGEG MEFFHHLMAEKPLL FLIPL LIAW+IERWVFS SNWVPL
Sbjct: 1   MARDGGPRGRKRGFFNGEGVMEFFHHLMAEKPLLRFLIPLVLIAWSIERWVFSLSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQRQLIVD+LN +WRR+ITNTSPETPLEPCAWLNKLL+EVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRQLIVDELNTKWRRIITNTSPETPLEPCAWLNKLLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTKF+STVNKRLKDRKSRLIEKIELL+FSLGSCPPSLGLSGTRWSTCG E         
Sbjct: 121 LSTKFTSTVNKRLKDRKSRLIEKIELLDFSLGSCPPSLGLSGTRWSTCGDE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKL KPFMGTARIVINSLHIKGDLVLMPILDG+AVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLGKPFMGTARIVINSLHIKGDLVLMPILDGRAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRC SLP VDLRKKAVGGI+
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCFSLPAVDLRKKAVGGIV 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCS-TNGSFGEHLTDKDMQTFVEVELEKLSRKTEAR 420
           YVTVISARKLYRSSLKGSPTRRQ S S  NGSFGEHLTDKDMQTFVEVELEKLSRKT+AR
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQQSYSANNGSFGEHLTDKDMQTFVEVELEKLSRKTDAR 420

Query: 421 SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIG 480
           SGSDPQWN+TFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKY ADDSTTFWAIG
Sbjct: 421 SGSDPQWNSTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYAADDSTTFWAIG 480

Query: 481 PDSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSL 540
           PDS V+A HADFCGKEVEM IPFEGAHCGEL VRLVLKEWMFSDGSHSSN YHV PQQSL
Sbjct: 481 PDSSVIAKHADFCGKEVEMDIPFEGAHCGELRVRLVLKEWMFSDGSHSSNRYHVSPQQSL 540

Query: 541 YGPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQ 600
           YG S+FL+STGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNP WNQ
Sbjct: 541 YGASSFLSSTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKALQRTRTAHSFNPTWNQ 600

Query: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRL 660
           KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGS+RDVWIPLEKVNSGELRL
Sbjct: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSVRDVWIPLEKVNSGELRL 660

Query: 661 QIEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTK 720
           QIEAIRVDDNEGSKGSSL P NGWIELVLIEA+DLVAADLRGTSDPYVRVQYGKLKKRTK
Sbjct: 661 QIEAIRVDDNEGSKGSSLAPTNGWIELVLIEARDLVAADLRGTSDPYVRVQYGKLKKRTK 720

Query: 721 VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIP 780
           VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIP
Sbjct: 721 VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIP 780

Query: 781 LQGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIE 840
           LQGVKRGEIHIQIT+R+PELDKRSSLDS+ SLDS++  N+A  ISSQMKQMM K Q+FIE
Sbjct: 781 LQGVKRGEIHIQITKRIPELDKRSSLDSKTSLDSDIHMNKAHHISSQMKQMMNKLQTFIE 837

Query: 841 DCNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
           D NLEGLATA+SELESLEDLQEEYMVQLETEQMLLINKIKELGQEFL+SSPSLSRRSSGY
Sbjct: 841 DSNLEGLATAMSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLNSSPSLSRRSSGY 837

BLAST of MS021645.1 vs. NCBI nr
Match: XP_023541948.1 (synaptotagmin-5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1518.8 bits (3931), Expect = 0.0e+00
Identity = 760/899 (84.54%), Postives = 793/899 (88.21%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           MSRA GPSGRKRGFFNG+GA EFF HLM +KPLLPFLIPL LIAW+IERWVFSFSNWVPL
Sbjct: 1   MSRAGGPSGRKRGFFNGDGAAEFFLHLMVDKPLLPFLIPLVLIAWSIERWVFSFSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQR+L+VDDLN +WRRL+TNTSPETPLEPCAWLNKLL+EVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRRLVVDDLNTKWRRLVTNTSPETPLEPCAWLNKLLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTK SSTVNKRL+DRKSRLIEKIELL+FSLGSCPPSLGLSG RWSTCG E         
Sbjct: 121 LSTKLSSTVNKRLRDRKSRLIEKIELLDFSLGSCPPSLGLSGARWSTCGDE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKLAK  MGTARIVINSLHIKGDLVLMPILDG+AVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLAKALMGTARIVINSLHIKGDLVLMPILDGRAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRC SLP VDLRKKAVGGII
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCFSLPSVDLRKKAVGGII 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420
           Y TV+SARKLYRSSLKGSPTRRQ S S NGSF E  TDKDMQTFVEVELEKLSRKTEARS
Sbjct: 361 YATVVSARKLYRSSLKGSPTRRQQSYSANGSFVEQFTDKDMQTFVEVELEKLSRKTEARS 420

Query: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480
           GSDPQWNTTFNMILHEDTGTLRFHLYEY PSHVKHDYLASCEVKMKY ADDSTTFWAIGP
Sbjct: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYRPSHVKHDYLASCEVKMKYAADDSTTFWAIGP 480

Query: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540
           DS V+A HADFCGKEVEMVIPFEGAH GEL+V+LVLKEWMFSDGSHSSN YHV PQQSL 
Sbjct: 481 DSSVIAKHADFCGKEVEMVIPFEGAHSGELTVKLVLKEWMFSDGSHSSNRYHVSPQQSLD 540

Query: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600
           G S+FL+STGRKI ITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNPIWNQK
Sbjct: 541 GASSFLSSTGRKIYITVVEGKDLPTKDKNGKCDPYVKLQYGKALQRTRTAHSFNPIWNQK 600

Query: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660
           FEFDEIAGGE+LKLKCLTEDIFGNDNTGSARVNLEGL EGS+RDVWIPLEKVNSGELRLQ
Sbjct: 601 FEFDEIAGGEHLKLKCLTEDIFGNDNTGSARVNLEGLAEGSVRDVWIPLEKVNSGELRLQ 660

Query: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720
           IEAIR+DDNEGSKGSSLGP NGWIELVLIEAKDLVAAD+RGTSDPYVRVQYGKLKKRTKV
Sbjct: 661 IEAIRMDDNEGSKGSSLGPTNGWIELVLIEAKDLVAADIRGTSDPYVRVQYGKLKKRTKV 720

Query: 721 MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780
           MYKTLSP+WNQ+LEFPDNGSPLLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIPL
Sbjct: 721 MYKTLSPKWNQILEFPDNGSPLLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIPL 780

Query: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 840
           QGVK+GEIHIQIT+RVPEL+KR+SLDSR SLDSE+P  RA +ISSQMKQMM K QSFIED
Sbjct: 781 QGVKKGEIHIQITKRVPELEKRTSLDSRTSLDSEIPLRRAHQISSQMKQMMNKLQSFIED 836

Query: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
            NLE ++T +SELESLEDLQEEYMVQLETEQMLLINKIKELGQEFL+SSPSLSRRSSGY
Sbjct: 841 SNLEEISTTVSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLNSSPSLSRRSSGY 836

BLAST of MS021645.1 vs. ExPASy Swiss-Prot
Match: A0JJX5 (Synaptotagmin-4 OS=Arabidopsis thaliana OX=3702 GN=SYT4 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.6e-20
Identity = 79/300 (26.33%), Postives = 135/300 (45.00%), Query Frame = 0

Query: 552 KINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTR----TAHSFNPIWNQKFEF-DEI 611
           K+++ VV+ KDL  KD  GK DPY  +       RT+     ++S NPIWN+ FEF  E 
Sbjct: 265 KLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRTKKTKTISNSLNPIWNEHFEFIVED 324

Query: 612 AGGEYLKLKCLTEDIFGNDN-TGSARVNLEGLVEGSIRDVWIPLEK--------VNSGEL 671
              ++L ++   ++  G+    G+A+V L  LV G ++D+W+ L K         N G++
Sbjct: 325 VSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKLVKDLEIQRDTKNRGQV 384

Query: 672 RLQ------------------------IEAIRVDDNEGSKGSSLGP----------ANGW 731
           +L+                        +E +   ++E S  + +              G 
Sbjct: 385 QLELLYCPLGKEGGLKNPFNPDYSLTILEKVLKPESEDSDATDMKKLVTSKKKDVIVRGV 444

Query: 732 IELVLIEAKDLVAADLRGTSDPYVRVQYGK--LKKRTKVMYKTLSPQWNQVLEFPDNGS- 791
           + + ++ A+DL A D  G +D +V +   K   K +T+V+  +L+P WNQ  +F    + 
Sbjct: 445 LSVTVVAAEDLPAVDFMGKADAFVVITLKKSETKSKTRVVPDSLNPVWNQTFDFVVEDAL 504

Query: 792 --PLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPLQGVKRGE--IHIQITRRV 797
              L L V DH+       IG   +    +     F +W  L G K G+  +H++ T R+
Sbjct: 505 HDLLTLEVWDHDK-FGKDKIGRVIMTLTRVMLEGEFQEWFELDGAKSGKLCVHLKWTPRL 563

BLAST of MS021645.1 vs. ExPASy Swiss-Prot
Match: K8FE10 (Synaptotagmin 2 OS=Caenorhabditis elegans OX=6239 GN=snt-2 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.6e-17
Identity = 74/277 (26.71%), Postives = 132/277 (47.65%), Query Frame = 0

Query: 552 KINITVVEGKDLPTKDKNGKCDPYVK---LQYGKGLQRTR-TAHSFNPIWNQKFE----F 611
           K+++T+V   DLP  D+NG  DPYVK   L   K    TR   ++ NP +N+ F+    F
Sbjct: 99  KLSVTIVSASDLPAMDRNGMSDPYVKVYVLPERKQKFETRIIRNTLNPTYNETFQFSIPF 158

Query: 612 DEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQIEA 671
           +E+     + +    + +  +D  G   V LE +  G   D+  PL+K    + +     
Sbjct: 159 NELHSKTLMLVVYDYDRLSKDDKMGQLSVPLESIDFGITTDIERPLQKPEKDDEK----E 218

Query: 672 IRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRV--QYGK---LKKRT 731
            R+ D   S  +   PA G + L ++EA++L   D+ G+SDPYV++   +G+    KK+T
Sbjct: 219 CRLGDICFS--TRYRPATGTVTLTIMEARNLKKMDVGGSSDPYVKIYLHHGRKLLSKKKT 278

Query: 732 KVMYKTLSPQWNQVLEFPDNGS-----PLLLHVKDHNALLPTSSIGDCAV--EYQGLP-- 791
              YKTL+P +N+  +F           L++ V D++ +     IG+  +  ++  LP  
Sbjct: 279 SRKYKTLNPYYNESFQFKIEPHMIEKVHLIVSVWDYDKMSKNDFIGEVTLGSKHLNLPQI 338

Query: 792 PNQMFDKWIPLQGVKRGEIHIQITRRVPELDKRSSLD 807
            +   ++W  +   +R  +     +   E +K+   D
Sbjct: 339 THACSEQWAEMMTSRRPVVQWHTLQERMEKEKKKDDD 369

BLAST of MS021645.1 vs. ExPASy Swiss-Prot
Match: Q9Z268 (RasGAP-activating-like protein 1 OS=Mus musculus OX=10090 GN=Rasal1 PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 1.4e-16
Identity = 73/246 (29.67%), Postives = 117/246 (47.56%), Query Frame = 0

Query: 546 LASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQY-GKGLQRTRTA-HSFNPIWNQKFEF 605
           +A +G  ++I VVEG+ LP KD +G  DPY  ++   + + RT T   S +P W +++  
Sbjct: 1   MAKSG-SLSIRVVEGRALPAKDVSGSSDPYCLVKVDDQVVARTATIWRSLSPFWGEEYTV 60

Query: 606 DEIAGGEYLKLKCLTEDIFGNDN-TGSARVNLEGL-VEGSIRDVWIPLEKVN-----SGE 665
                  +L    L ED  G+D+  G   ++ E +  +    D WI L +V+      GE
Sbjct: 61  HLPLDFHHLAFYVLDEDTVGHDDIIGKISLSKEAITADPRGIDSWINLSRVDPDAEVQGE 120

Query: 666 LRLQIEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKK 725
           + L ++ +     E ++G  L          + +A+DL   D+ GTSDP+ RV +G    
Sbjct: 121 VCLDVKLL-----EDARGRCL-------RCHVRQARDLAPRDISGTSDPFARVFWGNHSL 180

Query: 726 RTKVMYKTLSPQWNQVLEF---PDNGSPLLLHVKDHNALLPTSSIGDCAVEY-----QGL 775
            T  + KT  P W++VLE    P   SPL + + D + +     +G   VE+     Q  
Sbjct: 181 ETSTIKKTRFPHWDEVLELREAPGTTSPLRVELWDWDMVGKNDFLG--MVEFTPQTLQQK 231

BLAST of MS021645.1 vs. ExPASy Swiss-Prot
Match: O95294 (RasGAP-activating-like protein 1 OS=Homo sapiens OX=9606 GN=RASAL1 PE=1 SV=3)

HSP 1 Score: 89.0 bits (219), Expect = 3.0e-16
Identity = 62/208 (29.81%), Postives = 100/208 (48.08%), Query Frame = 0

Query: 553 INITVVEGKDLPTKDKNGKCDPYVKLQY-GKGLQRTRTA-HSFNPIWNQKFEFDEIAGGE 612
           +N+ VVEG+ LP KD +G  DPY  ++   + + RT T   S  P W +++         
Sbjct: 7   LNVRVVEGRALPAKDVSGSSDPYCLVKVDDEVVARTATVWRSLGPFWGEEYTVHLPLDFH 66

Query: 613 YLKLKCLTEDIFGNDN-TGSARVNLEGL-VEGSIRDVWIPLEKVN-----SGELRLQIEA 672
            L    L ED  G+D+  G   ++ E +  +    D WI L +V+      GE+ L ++ 
Sbjct: 67  QLAFYVLDEDTVGHDDIIGKISLSREAITADPRGIDSWINLSRVDPDAEVQGEICLSVQM 126

Query: 673 IRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKVMYK 732
           +  +D +G            +   +++A+DL   D+ GTSDP+ RV +G     T  + K
Sbjct: 127 L--EDGQGR----------CLRCHVLQARDLAPRDISGTSDPFARVFWGSQSLETSTIKK 186

Query: 733 TLSPQWNQVL---EFPDNGSPLLLHVKD 749
           T  P W++VL   E P   SPL + + D
Sbjct: 187 TRFPHWDEVLELREMPGAPSPLRVELWD 202

BLAST of MS021645.1 vs. ExPASy Swiss-Prot
Match: Q6PFQ7 (Ras GTPase-activating protein 4 OS=Mus musculus OX=10090 GN=Rasa4 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.2e-15
Identity = 69/250 (27.60%), Postives = 108/250 (43.20%), Query Frame = 0

Query: 553 INITVVEGKDLPTKDKNGKCDPY--VKLQYGKGLQRTRTAHSFNPIWNQKFEF------- 612
           ++I +VEGK+LP KD  G  DPY  VK+     ++      +  P W + ++        
Sbjct: 7   LSIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEDYQVHLPPTFH 66

Query: 613 ---------DEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNS 672
                    D ++  + +   CLT D   +   G +      LVE        P E+V  
Sbjct: 67  TVAFYVMDEDALSRDDVIGKVCLTRDALASHPKGFS--GWTHLVEVD------PNEEV-Q 126

Query: 673 GELRLQIEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKL 732
           GE+ L++E +      G   S L          ++EA+DL   D  G SDP+VRV Y   
Sbjct: 127 GEIHLRLEVV-----PGVHASRL-------RCAVLEARDLAPKDRNGASDPFVRVHYNGR 186

Query: 733 KKRTKVMYKTLSPQWNQVLEFP-DNGS--PLLLHVKDHNALLPTSSIGDCAVEYQGLPPN 782
            + T V+ K+  P+WN+  +F  + G+   LL+   D + +     +G  AV  Q L   
Sbjct: 187 TQETSVVKKSCYPRWNETFDFELEKGASEALLVEAWDWDLVSRNDFLGKVAVNVQRLCSA 235

BLAST of MS021645.1 vs. ExPASy TrEMBL
Match: A0A6J1C592 (synaptotagmin-5-like OS=Momordica charantia OX=3673 GN=LOC111008602 PE=4 SV=1)

HSP 1 Score: 1660.6 bits (4299), Expect = 0.0e+00
Identity = 835/899 (92.88%), Postives = 835/899 (92.88%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL
Sbjct: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGE         
Sbjct: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420
           YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420

Query: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480
           GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP
Sbjct: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480

Query: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540
           DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY
Sbjct: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540

Query: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600
           GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK
Sbjct: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600

Query: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660
           FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ
Sbjct: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660

Query: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720
           IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV
Sbjct: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720

Query: 721 MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780
           MYKTLSPQWNQVLEFPDN SPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL
Sbjct: 721 MYKTLSPQWNQVLEFPDNSSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780

Query: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 840
           QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED
Sbjct: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 836

Query: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
           CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY
Sbjct: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 836

BLAST of MS021645.1 vs. ExPASy TrEMBL
Match: A0A0A0LVR3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042330 PE=4 SV=1)

HSP 1 Score: 1545.0 bits (3999), Expect = 0.0e+00
Identity = 773/900 (85.89%), Postives = 800/900 (88.89%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           M+R  GP GRKRGFFNGEG MEFFHHLMAEKPLLPFLIPL LIAW+IERWVFS SNWVPL
Sbjct: 1   MARDGGPRGRKRGFFNGEGVMEFFHHLMAEKPLLPFLIPLVLIAWSIERWVFSLSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQRQLIVD+LN +WRR+ITNTSPETPLEPCAWLNKLL+EVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRQLIVDELNTKWRRIITNTSPETPLEPCAWLNKLLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTKF+STVNKRLKDRKSRLIEKIELL+FSLGSCPPSLGLSGTRWSTCG E         
Sbjct: 121 LSTKFTSTVNKRLKDRKSRLIEKIELLDFSLGSCPPSLGLSGTRWSTCGDE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKL KPFMGTARIVINSLHIKGDLVLMPILDG+AVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLGKPFMGTARIVINSLHIKGDLVLMPILDGRAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRC SLP VDLRKKAVGGI+
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCFSLPAVDLRKKAVGGIV 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCS-TNGSFGEHLTDKDMQTFVEVELEKLSRKTEAR 420
           YVTVISARKLYRSSLKGSPTRRQ S S  NGSFGEHLTDKDMQTFVEVELEKLSRKT+AR
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQQSYSANNGSFGEHLTDKDMQTFVEVELEKLSRKTDAR 420

Query: 421 SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIG 480
           SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKY ADDSTTFWAIG
Sbjct: 421 SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYAADDSTTFWAIG 480

Query: 481 PDSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSL 540
           PDS V+A +ADFCGKEVEM IPFEGAHCGEL VRLVLKEWMFSDGSHSSN YHV  QQSL
Sbjct: 481 PDSSVVAKYADFCGKEVEMDIPFEGAHCGELRVRLVLKEWMFSDGSHSSNRYHVSSQQSL 540

Query: 541 YGPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQ 600
           YG S+FL+STGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNP WNQ
Sbjct: 541 YGASSFLSSTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKALQRTRTAHSFNPTWNQ 600

Query: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRL 660
           KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGS+RDVWIPLEKVNSGELRL
Sbjct: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSVRDVWIPLEKVNSGELRL 660

Query: 661 QIEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTK 720
           QIEAIRVDDNEGSKGSSL P NGWIELVLIEA+DLVAAD+RGTSDPYVRVQYGKLKKRTK
Sbjct: 661 QIEAIRVDDNEGSKGSSLAPTNGWIELVLIEARDLVAADIRGTSDPYVRVQYGKLKKRTK 720

Query: 721 VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIP 780
           +MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIP
Sbjct: 721 IMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIP 780

Query: 781 LQGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIE 840
           LQGVKRGEIHIQIT+RVPELDKRSSLDS+ SLDSE P N+A ++SSQMKQMM K Q+FIE
Sbjct: 781 LQGVKRGEIHIQITKRVPELDKRSSLDSKTSLDSEFPMNKAHQVSSQMKQMMNKLQTFIE 837

Query: 841 DCNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
           D NLEGLATA+SELESLEDLQEEYMVQLE EQMLLINKIKELGQEFL+SSPSLSRRSSGY
Sbjct: 841 DSNLEGLATAMSELESLEDLQEEYMVQLENEQMLLINKIKELGQEFLNSSPSLSRRSSGY 837

BLAST of MS021645.1 vs. ExPASy TrEMBL
Match: A0A1S3C7V7 (synaptotagmin-5-like OS=Cucumis melo OX=3656 GN=LOC103497666 PE=4 SV=1)

HSP 1 Score: 1543.9 bits (3996), Expect = 0.0e+00
Identity = 774/900 (86.00%), Postives = 800/900 (88.89%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           M+R  GP GRKRGFFNGEG MEFFHHLMAEKPLL FLIPL LIAW+IERWVFS SNWVPL
Sbjct: 1   MARDGGPRGRKRGFFNGEGVMEFFHHLMAEKPLLRFLIPLVLIAWSIERWVFSLSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQRQLIVD+LN +WRR+ITNTSPETPLEPCAWLNKLL+EVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRQLIVDELNTKWRRIITNTSPETPLEPCAWLNKLLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTKF+STVNKRLKDRKSRLIEKIELL+FSLGSCPPSLGLSGTRWSTCG E         
Sbjct: 121 LSTKFTSTVNKRLKDRKSRLIEKIELLDFSLGSCPPSLGLSGTRWSTCGDE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKL KPFMGTARIVINSLHIKGDLVLMPILDG+AVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLGKPFMGTARIVINSLHIKGDLVLMPILDGRAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRC SLP VDLRKKAVGGI+
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCFSLPAVDLRKKAVGGIV 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCS-TNGSFGEHLTDKDMQTFVEVELEKLSRKTEAR 420
           YVTVISARKLYRSSLKGSPTRRQ S S  NGSFGEHLTDKDMQTFVEVELEKLSRKT+AR
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQQSYSANNGSFGEHLTDKDMQTFVEVELEKLSRKTDAR 420

Query: 421 SGSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIG 480
           SGSDPQWN+TFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKY ADDSTTFWAIG
Sbjct: 421 SGSDPQWNSTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYAADDSTTFWAIG 480

Query: 481 PDSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSL 540
           PDS V+A HADFCGKEVEM IPFEGAHCGEL VRLVLKEWMFSDGSHSSN YHV PQQSL
Sbjct: 481 PDSSVIAKHADFCGKEVEMDIPFEGAHCGELRVRLVLKEWMFSDGSHSSNRYHVSPQQSL 540

Query: 541 YGPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQ 600
           YG S+FL+STGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNP WNQ
Sbjct: 541 YGASSFLSSTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKALQRTRTAHSFNPTWNQ 600

Query: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRL 660
           KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGS+RDVWIPLEKVNSGELRL
Sbjct: 601 KFEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSVRDVWIPLEKVNSGELRL 660

Query: 661 QIEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTK 720
           QIEAIRVDDNEGSKGSSL P NGWIELVLIEA+DLVAADLRGTSDPYVRVQYGKLKKRTK
Sbjct: 661 QIEAIRVDDNEGSKGSSLAPTNGWIELVLIEARDLVAADLRGTSDPYVRVQYGKLKKRTK 720

Query: 721 VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIP 780
           VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIP
Sbjct: 721 VMYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIP 780

Query: 781 LQGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIE 840
           LQGVKRGEIHIQIT+R+PELDKRSSLDS+ SLDS++  N+A  ISSQMKQMM K Q+FIE
Sbjct: 781 LQGVKRGEIHIQITKRIPELDKRSSLDSKTSLDSDIHMNKAHHISSQMKQMMNKLQTFIE 837

Query: 841 DCNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
           D NLEGLATA+SELESLEDLQEEYMVQLETEQMLLINKIKELGQEFL+SSPSLSRRSSGY
Sbjct: 841 DSNLEGLATAMSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLNSSPSLSRRSSGY 837

BLAST of MS021645.1 vs. ExPASy TrEMBL
Match: A0A6J1GRB1 (synaptotagmin-5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456802 PE=4 SV=1)

HSP 1 Score: 1518.4 bits (3930), Expect = 0.0e+00
Identity = 760/899 (84.54%), Postives = 792/899 (88.10%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           MSRA GPSGRKRGFFNG+ A EFF HLM EKPLLPFLIPL LIAW+IERWVFSFSNWVPL
Sbjct: 1   MSRAGGPSGRKRGFFNGDDAAEFFLHLMVEKPLLPFLIPLVLIAWSIERWVFSFSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSYQR+L+VDDLN +WRRL+TNTSPETPLEPCAWLNKLL+EVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYQRRLVVDDLNTKWRRLVTNTSPETPLEPCAWLNKLLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LSTK SSTVNKRL+DRKSRLIEKIELL+FSLGSCPPSLGLSG RWSTCG E         
Sbjct: 121 LSTKLSSTVNKRLRDRKSRLIEKIELLDFSLGSCPPSLGLSGARWSTCGDE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHLS
Sbjct: 181 ------------------------------------------------------RIMHLS 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKLAK  MGTARIVINSLHIKGDLVLMPILDG+AVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLAKALMGTARIVINSLHIKGDLVLMPILDGRAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRC SLP VDLRKKAVGGII
Sbjct: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCFSLPSVDLRKKAVGGII 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420
           Y TV+SARKLYRSSLKGSP RRQ S S NGSF E  TDKDMQTFVEVELEKLSRKTEARS
Sbjct: 361 YATVVSARKLYRSSLKGSPPRRQQSYSANGSFVEQFTDKDMQTFVEVELEKLSRKTEARS 420

Query: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480
           GSDPQWNTTFNMILHEDTGTLRFHLYEY PSHVKHDYLASCEVKMKY ADDSTTFWAIGP
Sbjct: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYRPSHVKHDYLASCEVKMKYAADDSTTFWAIGP 480

Query: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540
           DS V+A HADFCGKEVEMVIPFEGAH GEL+VRLVLKEWMFSDGSHSSN YHV PQQSL 
Sbjct: 481 DSSVIAKHADFCGKEVEMVIPFEGAHSGELTVRLVLKEWMFSDGSHSSNRYHVSPQQSLD 540

Query: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600
           G S+FL+STGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNPIWNQK
Sbjct: 541 GASSFLSSTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKALQRTRTAHSFNPIWNQK 600

Query: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660
           FEFDEIAGGE+LKLKCLTEDIFGNDNTGSARVNLEGL EGS+RDVWIPLEKVNSGELRLQ
Sbjct: 601 FEFDEIAGGEHLKLKCLTEDIFGNDNTGSARVNLEGLAEGSVRDVWIPLEKVNSGELRLQ 660

Query: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720
           IEAIR+DDNEGSKGSSLGP NGWIELVLIEAKDLVAAD+RGTSDPYVRVQYGKLKKRTKV
Sbjct: 661 IEAIRMDDNEGSKGSSLGPTNGWIELVLIEAKDLVAADIRGTSDPYVRVQYGKLKKRTKV 720

Query: 721 MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780
           MYKTLSP+WNQ+LEFPDNGSPLLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIPL
Sbjct: 721 MYKTLSPKWNQILEFPDNGSPLLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIPL 780

Query: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 840
           QGVK+GEIHIQIT+RVPELDKR+SLDSR SLDSE+P  +A +ISSQMKQMM K QSFIED
Sbjct: 781 QGVKKGEIHIQITKRVPELDKRTSLDSRTSLDSEIPLRKAHQISSQMKQMMNKLQSFIED 836

Query: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSGY 900
            NLE ++T +SELESLEDLQEEYMVQLETEQMLLINKIKELGQEFL+SSPSLSR+SSGY
Sbjct: 841 SNLEEISTTVSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLNSSPSLSRKSSGY 836

BLAST of MS021645.1 vs. ExPASy TrEMBL
Match: A0A6J1E8M7 (synaptotagmin-5-like OS=Cucurbita moschata OX=3662 GN=LOC111431640 PE=4 SV=1)

HSP 1 Score: 1514.6 bits (3920), Expect = 0.0e+00
Identity = 752/898 (83.74%), Postives = 788/898 (87.75%), Query Frame = 0

Query: 1   MSRARGPSGRKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPL 60
           MSR  GPSGRKRG FNGEGA+EFF HLM EKP LPFLIPL L+AW+IERWVFS SNWVPL
Sbjct: 1   MSRVGGPSGRKRGLFNGEGALEFFRHLMREKPFLPFLIPLVLVAWSIERWVFSLSNWVPL 60

Query: 61  AVAVWATLQYGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPK 120
           AVAVWATLQYGSY+RQ+IVDDLN +WRRL+TNTSPETPLEPCAWLNKLL+EVWPNYFNPK
Sbjct: 61  AVAVWATLQYGSYERQVIVDDLNTKWRRLVTNTSPETPLEPCAWLNKLLMEVWPNYFNPK 120

Query: 121 LSTKFSSTVNKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDN 180
           LS+KFSS VNKRLKDRKSRLIE +ELLEFSLGSCPPSLGL G RW TCGGE         
Sbjct: 121 LSSKFSSAVNKRLKDRKSRLIENMELLEFSLGSCPPSLGLGGVRWLTCGGE--------- 180

Query: 181 MYFSSGNSFKELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLS 240
                                                                 RIMHL+
Sbjct: 181 ------------------------------------------------------RIMHLN 240

Query: 241 FDWDTNEMSILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300
           FDWDTNEMSILLQAKLAKP MGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI
Sbjct: 241 FDWDTNEMSILLQAKLAKPLMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRI 300

Query: 301 GVAFGSGGSQSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGII 360
           G+AFGSGGSQSLPATELPGVSSWLVK FTDTLVRTMVEPRRRC SLPPVDLRKKAVGGII
Sbjct: 301 GIAFGSGGSQSLPATELPGVSSWLVKFFTDTLVRTMVEPRRRCFSLPPVDLRKKAVGGII 360

Query: 361 YVTVISARKLYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARS 420
           YVTVISARKLYRSSLKGSPTRRQ S S+NGSFGEHL DKD+QTFVEVELEKLSRKT+ARS
Sbjct: 361 YVTVISARKLYRSSLKGSPTRRQQSHSSNGSFGEHLNDKDLQTFVEVELEKLSRKTDARS 420

Query: 421 GSDPQWNTTFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGP 480
           GSDPQWN TFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKY ADDSTTFWAIGP
Sbjct: 421 GSDPQWNATFNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYAADDSTTFWAIGP 480

Query: 481 DSRVLATHADFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLY 540
           +S V+A HA+FCGKEVEM IPFEGA CGELSVRLVLKEWM+SDGSHSSN YHV PQQSLY
Sbjct: 481 ESSVIARHAEFCGKEVEMDIPFEGAFCGELSVRLVLKEWMYSDGSHSSNRYHVSPQQSLY 540

Query: 541 GPSNFLASTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQK 600
           G S+F++STGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGK LQRTRTAHSFNP+WNQK
Sbjct: 541 GASSFVSSTGRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKSLQRTRTAHSFNPLWNQK 600

Query: 601 FEFDEIAGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQ 660
           FEFDEI GGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGS+RDVWIPLEKVNSGELRLQ
Sbjct: 601 FEFDEIGGGEYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSVRDVWIPLEKVNSGELRLQ 660

Query: 661 IEAIRVDDNEGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKV 720
           IEAIRVDDNEGS+GSSL P NGWIELVL+EA+DLVAADLRGTSDPYVRVQYGKLKKRTKV
Sbjct: 661 IEAIRVDDNEGSRGSSLAPTNGWIELVLVEARDLVAADLRGTSDPYVRVQYGKLKKRTKV 720

Query: 721 MYKTLSPQWNQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPL 780
           MYKTL PQWNQ+LEFPDNGS LLLHVKDHNALLPTSSIGDC VEYQGLPPNQMFDKWIPL
Sbjct: 721 MYKTLRPQWNQILEFPDNGSALLLHVKDHNALLPTSSIGDCVVEYQGLPPNQMFDKWIPL 780

Query: 781 QGVKRGEIHIQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIED 840
           QGVK+GEIHIQIT+RVPELDKRSSLDS+ SLDSE+  N+A +ISSQMKQMM K QSFIED
Sbjct: 781 QGVKKGEIHIQITKRVPELDKRSSLDSKTSLDSEISLNKAHRISSQMKQMMNKLQSFIED 835

Query: 841 CNLEGLATALSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPSLSRRSSG 899
            NLEGLAT +SELESLEDLQEEYM QLETEQMLLINKIKELGQEFL+SSPS SRRS G
Sbjct: 841 SNLEGLATGMSELESLEDLQEEYMAQLETEQMLLINKIKELGQEFLNSSPSSSRRSFG 835

BLAST of MS021645.1 vs. TAIR 10
Match: AT3G18370.1 (C2 domain-containing protein )

HSP 1 Score: 986.9 bits (2550), Expect = 1.1e-287
Identity = 507/882 (57.48%), Postives = 637/882 (72.22%), Query Frame = 0

Query: 10  RKRGFFNGEGAMEFFHHLMAEKPLLPFLIPLALIAWTIERWVFSFSNWVPLAVAVWATLQ 69
           +++G  N E A EF +HL+AE+  L  L+PL L  W IERWVF+FSNWVPL VAVWA+LQ
Sbjct: 6   KRKGLINTEAAREFINHLVAERHSLLLLVPLVLAFWAIERWVFAFSNWVPLVVAVWASLQ 65

Query: 70  YGSYQRQLIVDDLNKQWRRLITNTSPETPLEPCAWLNKLLLEVWPNYFNPKLSTKFSSTV 129
           YGSYQR L+ +DL K+WR+ + N S  TPLE C WLNKLL E+W NY N KLS +FSS V
Sbjct: 66  YGSYQRALLAEDLTKKWRQTVFNASTITPLEHCQWLNKLLSEIWLNYMNKKLSLRFSSMV 125

Query: 130 NKRLKDRKSRLIEKIELLEFSLGSCPPSLGLSGTRWSTCGGEVGLLFFLDNMYFSSGNSF 189
            KRL+ R+SRLIE I+LLEFSLGSCPP LGL GT WS  G +                  
Sbjct: 126 EKRLRQRRSRLIENIQLLEFSLGSCPPLLGLHGTCWSKSGEQ------------------ 185

Query: 190 KELPFFNDQAVELLSHLNFLNHFFGYDQCYLPIRFLFIGDDAIVKRIMHLSFDWDTNEMS 249
                                                        +IM L F+WDT ++S
Sbjct: 186 ---------------------------------------------KIMRLDFNWDTTDLS 245

Query: 250 ILLQAKLAKPFMGTARIVINSLHIKGDLVLMPILDGKAVLFSFVTTPDVRIGVAFGSGGS 309
           ILLQAKL+ PF  TARIV+NSL IKGD+++ PIL+G+A+L+SFV+ P+VRIGVAFG GG 
Sbjct: 246 ILLQAKLSMPFNRTARIVVNSLCIKGDILIRPILEGRALLYSFVSNPEVRIGVAFGGGGG 305

Query: 310 QSLPATELPGVSSWLVKIFTDTLVRTMVEPRRRCISLPPVDLRKKAVGGIIYVTVISARK 369
           QSLPATELPGVSSWLVKI T+TL + MVEPRR C SLP  DL K A+GGIIYVTV+S   
Sbjct: 306 QSLPATELPGVSSWLVKILTETLNKKMVEPRRGCFSLPATDLHKTAIGGIIYVTVVSGNN 365

Query: 370 LYRSSLKGSPTRRQHSCSTNGSFGEHLTDKDMQTFVEVELEKLSRKTEARSGSDPQWNTT 429
           L R  L+GSP++   S    GS G + + K +QTFVEVELE+LSR+TE +SG +P + +T
Sbjct: 366 LNRRILRGSPSK--SSEIGEGSSG-NSSSKPVQTFVEVELEQLSRRTEMKSGPNPAYQST 425

Query: 430 FNMILHEDTGTLRFHLYEYNPSHVKHDYLASCEVKMKYVADDSTTFWAIGPDSRVLATHA 489
           FNMILH++TGTL+F+LYE NP  V++D LASCEVKMKYV DDST FWA+G D+ V+A HA
Sbjct: 426 FNMILHDNTGTLKFNLYENNPGSVRYDSLASCEVKMKYVGDDSTMFWAVGSDNGVIAKHA 485

Query: 490 DFCGKEVEMVIPFEGAHCGELSVRLVLKEWMFSDGSHSSNSYHVRPQQSLYGPSNFLAST 549
           +FCG+E+EMV+PFEG   GEL+VRL+LKEW FSDGSHS NS +     SL   S  L+ T
Sbjct: 486 EFCGQEIEMVVPFEGVSSGELTVRLLLKEWHFSDGSHSLNSVNSSSLHSLDSSSALLSKT 545

Query: 550 GRKINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTRTAHSFNPIWNQKFEFDEIAGG 609
           GRKI +TV+ GK+L +KDK+GKCD  VKLQYGK +Q+T+  ++   +WNQKFEF+E+AG 
Sbjct: 546 GRKIIVTVLAGKNLVSKDKSGKCDASVKLQYGKIIQKTKIVNAAECVWNQKFEFEELAGE 605

Query: 610 EYLKLKCLTEDIFGNDNTGSARVNLEGLVEGSIRDVWIPLEKVNSGELRLQIEAIRVDDN 669
           EYLK+KC  E++ G DN G+A ++L+G +  S   +W+PLE VNSGE+ L IEA+   D 
Sbjct: 606 EYLKVKCYREEMLGTDNIGTATLSLQG-INNSEMHIWVPLEDVNSGEIELLIEAL---DP 665

Query: 670 EGSKGSSLGPANGWIELVLIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKVMYKTLSPQW 729
           E S+  S   + G IELVL+EA+DLVAAD+RGTSDPYVRVQYG+ K+RTKV+YKTL P+W
Sbjct: 666 EYSEADS---SKGLIELVLVEARDLVAADIRGTSDPYVRVQYGEKKQRTKVIYKTLQPKW 725

Query: 730 NQVLEFPDNGSPLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPLQGVKRGEIH 789
           NQ +EFPD+GS L LHVKD+N LLPTSSIG+C VEYQGL PN+  DKWI LQGVK GE+H
Sbjct: 726 NQTMEFPDDGSSLELHVKDYNTLLPTSSIGNCVVEYQGLKPNETADKWIILQGVKHGEVH 785

Query: 790 IQITRRVPELDKRSSLDSRPSLDSEMPSNRAQKISSQMKQMMIKFQSFIEDCNLEGLATA 849
           +++TR+V E+ +R+      S     P N+A  +S+QMKQ+MIKFQ+ I+D +LEGLA A
Sbjct: 786 VRVTRKVTEIQRRA------SAGPGTPFNKALLLSNQMKQVMIKFQNLIDDGDLEGLAEA 808

Query: 850 LSELESLEDLQEEYMVQLETEQMLLINKIKELGQEFLSSSPS 892
           L ELESLED QE+Y++QL+TEQ LLINKIK+LG+E L+SSP+
Sbjct: 846 LEELESLEDEQEQYLLQLQTEQSLLINKIKDLGKEILNSSPA 808

BLAST of MS021645.1 vs. TAIR 10
Match: AT5G11100.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 102.4 bits (254), Expect = 1.9e-21
Identity = 79/300 (26.33%), Postives = 135/300 (45.00%), Query Frame = 0

Query: 552 KINITVVEGKDLPTKDKNGKCDPYVKLQYGKGLQRTR----TAHSFNPIWNQKFEF-DEI 611
           K+++ VV+ KDL  KD  GK DPY  +       RT+     ++S NPIWN+ FEF  E 
Sbjct: 265 KLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRTKKTKTISNSLNPIWNEHFEFIVED 324

Query: 612 AGGEYLKLKCLTEDIFGNDN-TGSARVNLEGLVEGSIRDVWIPLEK--------VNSGEL 671
              ++L ++   ++  G+    G+A+V L  LV G ++D+W+ L K         N G++
Sbjct: 325 VSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKLVKDLEIQRDTKNRGQV 384

Query: 672 RLQ------------------------IEAIRVDDNEGSKGSSLGP----------ANGW 731
           +L+                        +E +   ++E S  + +              G 
Sbjct: 385 QLELLYCPLGKEGGLKNPFNPDYSLTILEKVLKPESEDSDATDMKKLVTSKKKDVIVRGV 444

Query: 732 IELVLIEAKDLVAADLRGTSDPYVRVQYGK--LKKRTKVMYKTLSPQWNQVLEFPDNGS- 791
           + + ++ A+DL A D  G +D +V +   K   K +T+V+  +L+P WNQ  +F    + 
Sbjct: 445 LSVTVVAAEDLPAVDFMGKADAFVVITLKKSETKSKTRVVPDSLNPVWNQTFDFVVEDAL 504

Query: 792 --PLLLHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPLQGVKRGE--IHIQITRRV 797
              L L V DH+       IG   +    +     F +W  L G K G+  +H++ T R+
Sbjct: 505 HDLLTLEVWDHDK-FGKDKIGRVIMTLTRVMLEGEFQEWFELDGAKSGKLCVHLKWTPRL 563

BLAST of MS021645.1 vs. TAIR 10
Match: AT3G14590.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 87.0 bits (214), Expect = 8.2e-17
Identity = 40/110 (36.36%), Postives = 66/110 (60.00%), Query Frame = 0

Query: 688 LIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKVMYKTLSPQWNQVLEFP----DNGSPLL 747
           ++EA D+  +DL G +DPYV+ Q G  + +TK+++KTL+P+W +  + P    D+ + L 
Sbjct: 244 VVEACDVKPSDLNGLADPYVKGQLGAYRFKTKILWKTLAPKWQEEFKIPICTWDSANILN 303

Query: 748 LHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPLQGVKRGEIHIQIT 794
           + V+D +      S+GDC+V        Q  D W+PLQ +K G +H+ IT
Sbjct: 304 IEVQDKDR-FSDDSLGDCSVNIAEFRGGQRNDMWLPLQNIKMGRLHLAIT 352

BLAST of MS021645.1 vs. TAIR 10
Match: AT3G14590.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 87.0 bits (214), Expect = 8.2e-17
Identity = 40/110 (36.36%), Postives = 66/110 (60.00%), Query Frame = 0

Query: 688 LIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKVMYKTLSPQWNQVLEFP----DNGSPLL 747
           ++EA D+  +DL G +DPYV+ Q G  + +TK+++KTL+P+W +  + P    D+ + L 
Sbjct: 289 VVEACDVKPSDLNGLADPYVKGQLGAYRFKTKILWKTLAPKWQEEFKIPICTWDSANILN 348

Query: 748 LHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPLQGVKRGEIHIQIT 794
           + V+D +      S+GDC+V        Q  D W+PLQ +K G +H+ IT
Sbjct: 349 IEVQDKDR-FSDDSLGDCSVNIAEFRGGQRNDMWLPLQNIKMGRLHLAIT 397

BLAST of MS021645.1 vs. TAIR 10
Match: AT3G14590.3 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 87.0 bits (214), Expect = 8.2e-17
Identity = 40/110 (36.36%), Postives = 66/110 (60.00%), Query Frame = 0

Query: 688 LIEAKDLVAADLRGTSDPYVRVQYGKLKKRTKVMYKTLSPQWNQVLEFP----DNGSPLL 747
           ++EA D+  +DL G +DPYV+ Q G  + +TK+++KTL+P+W +  + P    D+ + L 
Sbjct: 258 VVEACDVKPSDLNGLADPYVKGQLGAYRFKTKILWKTLAPKWQEEFKIPICTWDSANILN 317

Query: 748 LHVKDHNALLPTSSIGDCAVEYQGLPPNQMFDKWIPLQGVKRGEIHIQIT 794
           + V+D +      S+GDC+V        Q  D W+PLQ +K G +H+ IT
Sbjct: 318 IEVQDKDR-FSDDSLGDCSVNIAEFRGGQRNDMWLPLQNIKMGRLHLAIT 366