MS019065.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS019065.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Methenyltetrahydrofolate cyclohydrolase
Location	scaffold20: 1151888 .. 1154302 (+)
Sequence length	930
RNA-Seq Expression	MS019065.1
Synteny	MS019065.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCCCTTGTCACCTGCAAAAATGGCGTCCGATTCCGGCCACAAAGCTACGATAATCGACGGCAAAGAAATCGCCCAGACCGTCAGATCTGAAATCGCGGAGGAAGTGAGCAAACTCTCAGAGAAATACGGAAAGGTTTTTTTTTATCAATTTATATCTGATAATTGTGAAACAATTTATGTGTTTAGCCGCGTCTGATTTCAGCATTTTGATCTTGTTCCTTTTGGTGTGGGATTTGCGAAGGTTCCGGGACTGGCGGAGGTGATTATCGGGAGTAGAAAGTGTACACTGAACTATGTAAATATGAAGAGAAAGGCTTGCGTGGAAGTCGGAATCAAGTACTTCGGTTTTGATCTTCCTGAACAAGTGTCTGAGGCCGAATTGATCGCCAAGATCCATGAGTTGAATGCGAATCCTGAAGTACATGGTTAATCTCTTCTCTACTTTTTGAATTCCTTGTGCCAACAATTGCATTGTTATATTGTAGTAAACGTCTTTCTGCTAGATAAATGTTGTTTCCATTAATTTTTCCAATCTTTAATAAATTTAATTGGAGCTTTTTATCTTTCTCCAAGCATATAGTTTATTCTTCTGAGAGCATCGCTTTTTAGTACTTTGCTTTTAAGTTTTTTTTTCAAGTCTTCGAAAAAATTCAGATGTGGCTACAAGTAGTTTTCACACTGTTCTAGAAGTTATACTAAGCTTACCCTTATTATGTGGTATTGGGGTTTTTGCATTTGAAGTTTTTTTTCTTTTTAATCCTAAGAAACTTGAAAACTTCCGTTCGAATTTTCTCCCCCAACAAATTTATTGGTTTGTTGCTTTCACAGGTATATTGGTTCAGCTTCGTTTGCCAAGGCACATAAATGAAGAGAAAGTTTTAGCTGAAATAAGCATCGAGAAGGATATAGATGGCTTTCATCCTCTGAACATCGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTATTCCTACCTTGCACTCCAAAGGCAATAACAAATGTTTATAAGCCTTAGAGAATAATCATTAAACTCATAAATCTTTGTCAACTGGAAGAGCAGTAGCTCCTTGTATATCATTGTTCAAAGATTCCGTTTCGTTTCGTTTCATTTTTATGTTGATCATAGCTCAGAAAATGGTAACTCATGTCATAAGTTCACTTCTGAGGTAATAATGTTTTATTATGTGCAGGGGTGTCTTGAGTTGCTGTCACGAAGTGGAATAAGTATTAGGGGAAAGAAGGCTGTTGTTCTGGGGCGAAGCAACATAGTTGGATTACCAGTTTCTTTGCTGCTTCTGAAAGCAGATGCAACCGTGACGATTGTTCATTCGCTTTCTGTTGATCCAGAAAGCGTTATCCGGGAAGCAGATATCATAATTGCTGCAGCCGGACTGGCACAAATGGTATGCTAGATTTTACAATGGCATTGTTTTCCCCTCCCCAATTCTTATATTTGAATATCTGTTTGGCTTCACCGAGGATCTCAGACTTTGGTTTCTAAGTTTACTGGAAAATGATAGGAAGTATGTTTAGGTGCTACATTAGTATTAGAAAAATAAAAGTTAATTTACAAATTTAGTCCCCAGAGATTCATGTTTATTTCTATTTAGTCCTTAAATTTTAATAACTTTCAATTAAAATTTTGGTTCTATTTATTAGACAAATCTTAAATGGTTTGACATGAAGTGCTAGACAAATGGTCGAAAGAAATTGATAGTGAAACAAAATAGAGTGACACTTTTATAGATACGTTGCTTAAAGCACATGCCACTTTATTTGGTTGAATTATCTACTTTCTTCAGCCATCTATCGACCCCATTGTGCCAAATCAGCCAACCATCTGTCTAATAAGTATGCAAAATCATTATTTAGTTAACAATCTTAATAGTAGAACTAATTAGAATCGTAATTCAAAGTCCAGGAACAGTAATTAGAAGTTTTTTAGAACTTATATACTAAATAAACCTTACCCTAAAAGATTTAAAGTCGTAATTTAATCTATGGAGAGATCAAACTTTATCATATGAATGTGAATGATGGCTTTTGCACCAGTGCAATGATGTCTTCTGGTTGCTAATATTCCTAACATATCTTTTTTTCTCCATTTTTATTTTTTATTTTTTTTGGAACAACTTTGTGAGAGACTTAATTACTGATGCATATTGTTGGTGGGTTGTTTGATGTTAGATCAAGGGCAGTTGGATCAAACCGGGTGCTGCAGTCATTGATGTTGGTACAAATGCAGTTGATGACCCAACCACAAAGTCGGGCTACCGGCTGGTTGGGGATGTAGATTTCCAGGAAGCTTGTAAAGTGGCTGGTTGGATAACGCCTGTTCCCGGTGGTGTCGGCCCAATGGCCGTCGCCATGATGCTCAGGAATACTCTGGATGGCGCTAAGCGGGTGATCGAGCAG

mRNA sequence

TTCCCCTTGTCACCTGCAAAAATGGCGTCCGATTCCGGCCACAAAGCTACGATAATCGACGGCAAAGAAATCGCCCAGACCGTCAGATCTGAAATCGCGGAGGAAGTGAGCAAACTCTCAGAGAAATACGGAAAGGTTCCGGGACTGGCGGAGGTGATTATCGGGAGTAGAAAGTGTACACTGAACTATGTAAATATGAAGAGAAAGGCTTGCGTGGAAGTCGGAATCAAGTACTTCGGTTTTGATCTTCCTGAACAAGTGTCTGAGGCCGAATTGATCGCCAAGATCCATGAGTTGAATGCGAATCCTGAAGTACATGGTATATTGGTTCAGCTTCGTTTGCCAAGGCACATAAATGAAGAGAAAGTTTTAGCTGAAATAAGCATCGAGAAGGATATAGATGGCTTTCATCCTCTGAACATCGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTATTCCTACCTTGCACTCCAAAGGCAATAACAAATGGGTGTCTTGAGTTGCTGTCACGAAGTGGAATAAGTATTAGGGGAAAGAAGGCTGTTGTTCTGGGGCGAAGCAACATAGTTGGATTACCAGTTTCTTTGCTGCTTCTGAAAGCAGATGCAACCGTGACGATTGTTCATTCGCTTTCTGTTGATCCAGAAAGCGTTATCCGGGAAGCAGATATCATAATTGCTGCAGCCGGACTGGCACAAATGATCAAGGGCAGTTGGATCAAACCGGGTGCTGCAGTCATTGATGTTGGTACAAATGCAGTTGATGACCCAACCACAAAGTCGGGCTACCGGCTGGTTGGGGATGTAGATTTCCAGGAAGCTTGTAAAGTGGCTGGTTGGATAACGCCTGTTCCCGGTGGTGTCGGCCCAATGGCCGTCGCCATGATGCTCAGGAATACTCTGGATGGCGCTAAGCGGGTGATCGAGCAG

Coding sequence (CDS)

TTCCCCTTGTCACCTGCAAAAATGGCGTCCGATTCCGGCCACAAAGCTACGATAATCGACGGCAAAGAAATCGCCCAGACCGTCAGATCTGAAATCGCGGAGGAAGTGAGCAAACTCTCAGAGAAATACGGAAAGGTTCCGGGACTGGCGGAGGTGATTATCGGGAGTAGAAAGTGTACACTGAACTATGTAAATATGAAGAGAAAGGCTTGCGTGGAAGTCGGAATCAAGTACTTCGGTTTTGATCTTCCTGAACAAGTGTCTGAGGCCGAATTGATCGCCAAGATCCATGAGTTGAATGCGAATCCTGAAGTACATGGTATATTGGTTCAGCTTCGTTTGCCAAGGCACATAAATGAAGAGAAAGTTTTAGCTGAAATAAGCATCGAGAAGGATATAGATGGCTTTCATCCTCTGAACATCGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTATTCCTACCTTGCACTCCAAAGGCAATAACAAATGGGTGTCTTGAGTTGCTGTCACGAAGTGGAATAAGTATTAGGGGAAAGAAGGCTGTTGTTCTGGGGCGAAGCAACATAGTTGGATTACCAGTTTCTTTGCTGCTTCTGAAAGCAGATGCAACCGTGACGATTGTTCATTCGCTTTCTGTTGATCCAGAAAGCGTTATCCGGGAAGCAGATATCATAATTGCTGCAGCCGGACTGGCACAAATGATCAAGGGCAGTTGGATCAAACCGGGTGCTGCAGTCATTGATGTTGGTACAAATGCAGTTGATGACCCAACCACAAAGTCGGGCTACCGGCTGGTTGGGGATGTAGATTTCCAGGAAGCTTGTAAAGTGGCTGGTTGGATAACGCCTGTTCCCGGTGGTGTCGGCCCAATGGCCGTCGCCATGATGCTCAGGAATACTCTGGATGGCGCTAAGCGGGTGATCGAGCAG

Protein sequence

FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRVIEQ

Homology

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BLAST of MS019065.1 vs. NCBI nr
Match: XP_022143448.1 (bifunctional protein FolD 2-like [Momordica charantia])

HSP 1 Score: 581.3 bits (1497), Expect = 5.1e-162
Identity = 298/310 (96.13%), Postives = 303/310 (97.74%), Query Frame = 0


Query: 1   FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCT 60
           FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVI+GSRKC+
Sbjct: 85  FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIVGSRKCS 144

Query: 61  LNYVNMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE 120
           L+YVN+KRKAC EVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE
Sbjct: 145 LSYVNLKRKACAEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE 204

Query: 121 EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKK 180
           EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPK    GCLELLSRSGISIRGKK
Sbjct: 205 EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPK----GCLELLSRSGISIRGKK 264

Query: 181 AVVLGRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWI 240
           AVVLGRSNIVG+PVSLLLLKADATVTIVHSLS DPESVIREADIIIAAAGLAQMIKGSWI
Sbjct: 265 AVVLGRSNIVGIPVSLLLLKADATVTIVHSLSGDPESVIREADIIIAAAGLAQMIKGSWI 324

Query: 241 KPGAAVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT 300
           KPGA VIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT
Sbjct: 325 KPGATVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT 384

Query: 301 LDGAKRVIEQ 311
           LDGAKRVIEQ
Sbjct: 385 LDGAKRVIEQ 390

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BLAST of MS019065.1 vs. NCBI nr
Match: KAA0044358.1 (bifunctional protein FolD 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 539.7 bits (1389), Expect = 1.7e-149
Identity = 274/303 (90.43%), Postives = 288/303 (95.05%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MAS+S HKATIIDGK+IAQTVRSE+AEEV+KLSEKYGKVPGLA VI+G+RK +L YVNMK
Sbjct: 1   MASESDHKATIIDGKKIAQTVRSEVAEEVNKLSEKYGKVPGLAVVIVGNRKDSLTYVNMK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC+EVGIK F  DLPEQVSEAELI+K+HELNANPEVHGILVQL LP HINEEKVL+EI
Sbjct: 61  RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPKAI NGCLELLSRSGISIRGKKAVV+GRS
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKAIMNGCLELLSRSGISIRGKKAVVMGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVTIVHS SVDPESVIREADIIIAAAG AQMIKGSWIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNTLDGAKR 
Sbjct: 241 DVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRA 300

Query: 308 IEQ 311
           IEQ
Sbjct: 301 IEQ 303

[Close]

BLAST of MS019065.1 vs. NCBI nr
Match: XP_004152261.2 (bifunctional protein FolD 2 [Cucumis sativus])

HSP 1 Score: 535.4 bits (1378), Expect = 3.2e-148
Identity = 274/310 (88.39%), Postives = 290/310 (93.55%), Query Frame = 0


Query: 1   FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCT 60
           F  SPAKMAS+S HKATIIDGK+IAQTVRSEI EEV+KLS+KYGK+PGLA VI+G+RK +
Sbjct: 68  FFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDS 127

Query: 61  LNYVNMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE 120
           L YVNMKRKAC+EVGIK F  DLPEQVSEAELI+K+HELNANPEVHGILVQL LP HINE
Sbjct: 128 LTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINE 187

Query: 121 EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKK 180
           EKVL+EISIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPK    GC+ELLSRSGISIRGKK
Sbjct: 188 EKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPK----GCIELLSRSGISIRGKK 247

Query: 181 AVVLGRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWI 240
           AVV+GRSNIVGLPVSLLLLKADATVTIVHS SVDPESVIREADIIIAAAG AQMIKGSWI
Sbjct: 248 AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWI 307

Query: 241 KPGAAVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT 300
           KPGAAVIDVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNT
Sbjct: 308 KPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT 367

Query: 301 LDGAKRVIEQ 311
           LDGAKRVIEQ
Sbjct: 368 LDGAKRVIEQ 373

[Close]

BLAST of MS019065.1 vs. NCBI nr
Match: XP_022143417.1 (bifunctional protein FolD 2-like [Momordica charantia])

HSP 1 Score: 533.1 bits (1372), Expect = 1.6e-147
Identity = 273/303 (90.10%), Postives = 286/303 (94.39%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MASDSGHKAT+IDGKEIAQ VRSEIAEEV+KLSEK+GKVPGLA VI+GSRK + +YV MK
Sbjct: 1   MASDSGHKATLIDGKEIAQAVRSEIAEEVNKLSEKHGKVPGLAVVIVGSRKDSQSYVKMK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC EVGIK F FDLPEQVSEAEL+AKIHELNANPEVHGILVQL LP+HINEEKVLAEI
Sbjct: 61  RKACAEVGIKSFDFDLPEQVSEAELVAKIHELNANPEVHGILVQLPLPKHINEEKVLAEI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPK    GCLELLSRSGISIRGKKAVV+GRS
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPK----GCLELLSRSGISIRGKKAVVMGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVTIVHS SVDPESV+READI+IAAAG AQMIKGSWIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTIVHSRSVDPESVVREADIVIAAAGQAQMIKGSWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNTLDGAKRV
Sbjct: 241 DVGTNAVDDPTRKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 299

Query: 308 IEQ 311
           IEQ
Sbjct: 301 IEQ 299

[Close]

BLAST of MS019065.1 vs. NCBI nr
Match: XP_008454369.1 (PREDICTED: bifunctional protein FolD 2 isoform X1 [Cucumis melo] >TYK29487.1 bifunctional protein FolD 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 528.5 bits (1360), Expect = 3.9e-146
Identity = 271/303 (89.44%), Postives = 285/303 (94.06%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MAS+S HKATIIDGK+IAQTVRSE+AEEV+KLSEKYGKVPGLA VI+G+RK +L YVNMK
Sbjct: 1   MASESDHKATIIDGKKIAQTVRSEVAEEVNKLSEKYGKVPGLAVVIVGNRKDSLTYVNMK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC+EVGIK F  DLPEQVSEAELI+K+HELNANPEVHGILVQL LP HINEEKVL+EI
Sbjct: 61  RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPK    GCLELLSRSGISIRGKKAVV+GRS
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPK----GCLELLSRSGISIRGKKAVVMGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVTIVHS SVDPESVIREADIIIAAAG AQMIKGSWIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNTLDGAKR 
Sbjct: 241 DVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRA 299

Query: 308 IEQ 311
           IEQ
Sbjct: 301 IEQ 299

[Close]

BLAST of MS019065.1 vs. ExPASy Swiss-Prot
Match: Q9LHH7 (Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 8.6e-128
Identity = 232/300 (77.33%), Postives = 262/300 (87.33%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MAS S H A IIDGK IA T+RSEIAEEV  LSEK+GKVPGLA VI+GSRK +  YVN K
Sbjct: 1   MASSSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC EVGIK F   LPE+VSEA+LI+K+HELN+NP+VHGILVQL LP+HINEE +L  I
Sbjct: 61  RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SI+KD+DGFHPLNIGKLAMKGR+PLFLPCTPK    GCLELL+RSG+ I+G++AVV+GRS
Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPK----GCLELLARSGVKIKGQRAVVVGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVT VHS + DPE++IREADI+IAA G A MIKG+WIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAV DP+ KSGYRLVGDVDF EA KVAG+ITPVPGGVGPM VAM+LRNT+DGAKRV
Sbjct: 241 DVGTNAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKRV 296

[Close]

BLAST of MS019065.1 vs. ExPASy Swiss-Prot
Match: O65271 (Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD4 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 8.6e-96
Identity = 173/306 (56.54%), Postives = 225/306 (73.53%), Query Frame = 0


Query: 2   PLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTL 61
           P+S     + S   A +IDGK +A+ +R EI  EVS++ E  G +PGLA +++G RK + 
Sbjct: 55  PISSISADTKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSA 114

Query: 62  NYVNMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEE 121
            YV  K+KAC  VGIK F   L E  SE E++  +   N +P VHGILVQL LP H++E+
Sbjct: 115 TYVRNKKKACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQ 174

Query: 122 KVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKA 181
            +L  +SIEKD+DGFHPLNIG+LAM+GR+PLF+PCTPK    GC+ELL R  I I+GK+A
Sbjct: 175 NILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPK----GCIELLHRYNIEIKGKRA 234

Query: 182 VVLGRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIK 241
           VV+GRSNIVG+P +LLL + DATV+I+HS + +PE + READIII+A G   M++GSWIK
Sbjct: 235 VVIGRSNIVGMPAALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIK 294

Query: 242 PGAAVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTL 301
           PGA +IDVG N V+DP+   GYRLVGD+ ++EA KVA  ITPVPGGVGPM +AM+L NTL
Sbjct: 295 PGAVLIDVGINPVEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTL 354

Query: 302 DGAKRV 308
             AKR+
Sbjct: 355 TSAKRI 356

[Close]

BLAST of MS019065.1 vs. ExPASy Swiss-Prot
Match: A2RVV7 (Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FOLD1 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.4e-92
Identity = 164/303 (54.13%), Postives = 225/303 (74.26%), Query Frame = 0


Query: 5   PAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYV 64
           P  ++ ++  K  +IDG  IA+ +R++I  EV K+ +  GKVPGLA V++G ++ +  YV
Sbjct: 52  PPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYV 111

Query: 65  NMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVL 124
             K KAC E GIK    +LPE  +E ++I+ + + N +  +HGILVQL LP+H+NE K+L
Sbjct: 112 RNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKIL 171

Query: 125 AEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVL 184
             + +EKD+DGFHPLN+G LAM+GR+PLF+ CTPK    GC+ELL R+G+ I GK AVV+
Sbjct: 172 NMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPK----GCVELLIRTGVEIAGKNAVVI 231

Query: 185 GRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGA 244
           GRSNIVGLP+SLLL + DATV+ VH+ + DPE + R+ADI+IAAAG+  +++GSW+KPGA
Sbjct: 232 GRSNIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGA 291

Query: 245 AVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGA 304
            VIDVGT  V+D + + GYRLVGDV ++EA  VA  ITPVPGGVGPM + M+L NTL+ A
Sbjct: 292 VVIDVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAA 350

Query: 305 KRV 308
           KR+
Sbjct: 352 KRI 350

[Close]

BLAST of MS019065.1 vs. ExPASy Swiss-Prot
Match: O65269 (Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD3 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.9e-75
Identity = 147/298 (49.33%), Postives = 194/298 (65.10%), Query Frame = 0


Query: 10  SDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMKRK 69
           S S   A +IDGK  A+ +R +I  EVS++ E  G VP                      
Sbjct: 49  SSSSPSAIVIDGKAEAKKIRDDIKIEVSRMKESIGVVPA--------------------- 108

Query: 70  ACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEISI 129
                          E  SE E++  +   N +P VHG+LVQL LP H++E+ +L  +SI
Sbjct: 109 ---------------EDSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSI 168

Query: 130 EKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRSNI 189
           EKD+DGFHPLNIG+LAM+GR+PLF+PCTPK    GC+ELL R  I  +GK+AVV+GRSNI
Sbjct: 169 EKDVDGFHPLNIGRLAMRGREPLFVPCTPK----GCIELLHRYNIEFKGKRAVVIGRSNI 228

Query: 190 VGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVIDV 249
           VG+P +LLL K DATV+I+HS +++PE + R+ADI+I+A G   M++GSWIKPGA +IDV
Sbjct: 229 VGMPAALLLQKEDATVSIIHSRTMNPEELTRQADILISAVGKPNMVRGSWIKPGAVLIDV 288

Query: 250 GTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 308
           G   V+DP+   G RLVGD+ + EA K+A  ITPVPG VGPM +AM+L NTL  AKR+
Sbjct: 289 GIKPVEDPSAAGGERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKRI 306

[Close]

BLAST of MS019065.1 vs. ExPASy Swiss-Prot
Match: A0LE04 (Bifunctional protein FolD OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1) OX=156889 GN=folD PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.6e-73
Identity = 151/291 (51.89%), Postives = 194/291 (66.67%), Query Frame = 0


Query: 16  ATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMKRKACVEVG 75
           A +IDGK IAQ+VR E+  EV +L   +   PGLA V++G+   +  YV  K++AC   G
Sbjct: 2   AHVIDGKAIAQSVREELRMEVERLKLNHQLTPGLAVVLVGADPASQVYVRNKKRACETAG 61

Query: 76  IKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEISIEKDIDG 135
           I  F  +L    S+AEL+A I +LN +  VHGILVQL LP+HI+E+KVL  IS  KD DG
Sbjct: 62  IASFSHELAATTSQAELLALIEQLNQDDAVHGILVQLPLPKHIDEQKVLEAISPSKDADG 121

Query: 136 FHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRSNIVGLPVS 195
           FHP N+G+L     DP F PCTP     G +E+L  SG+  +GK AVV+GRSNIVG PV+
Sbjct: 122 FHPYNVGRLVT--GDPTFQPCTPW----GVMEMLKVSGVDPKGKHAVVIGRSNIVGKPVA 181

Query: 196 LLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVIDVGTNAVD 255
           L+LL A ATVTI HS + D    ++ ADI++AA G A M+ GSWIK GA VIDVG N  +
Sbjct: 182 LMLLAAHATVTICHSRTPDLAETVKRADIVVAAVGRANMVPGSWIKKGAVVIDVGINRGE 241

Query: 256 DPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKR 307
           D       +L GDVD+    + A  ITPVPGGVGPM +AM+L+NT++GAKR
Sbjct: 242 DG------KLCGDVDYASCFEHASAITPVPGGVGPMTIAMLLKNTVEGAKR 280

[Close]

BLAST of MS019065.1 vs. ExPASy TrEMBL
Match: A0A6J1CQR1 (bifunctional protein FolD 2-like OS=Momordica charantia OX=3673 GN=LOC111013324 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.5e-162
Identity = 298/310 (96.13%), Postives = 303/310 (97.74%), Query Frame = 0


Query: 1   FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCT 60
           FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVI+GSRKC+
Sbjct: 85  FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIVGSRKCS 144

Query: 61  LNYVNMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE 120
           L+YVN+KRKAC EVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE
Sbjct: 145 LSYVNLKRKACAEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE 204

Query: 121 EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKK 180
           EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPK    GCLELLSRSGISIRGKK
Sbjct: 205 EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPK----GCLELLSRSGISIRGKK 264

Query: 181 AVVLGRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWI 240
           AVVLGRSNIVG+PVSLLLLKADATVTIVHSLS DPESVIREADIIIAAAGLAQMIKGSWI
Sbjct: 265 AVVLGRSNIVGIPVSLLLLKADATVTIVHSLSGDPESVIREADIIIAAAGLAQMIKGSWI 324

Query: 241 KPGAAVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT 300
           KPGA VIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT
Sbjct: 325 KPGATVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT 384

Query: 301 LDGAKRVIEQ 311
           LDGAKRVIEQ
Sbjct: 385 LDGAKRVIEQ 390

[Close]

BLAST of MS019065.1 vs. ExPASy TrEMBL
Match: A0A5A7TNB3 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00850 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.3e-150
Identity = 274/303 (90.43%), Postives = 288/303 (95.05%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MAS+S HKATIIDGK+IAQTVRSE+AEEV+KLSEKYGKVPGLA VI+G+RK +L YVNMK
Sbjct: 1   MASESDHKATIIDGKKIAQTVRSEVAEEVNKLSEKYGKVPGLAVVIVGNRKDSLTYVNMK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC+EVGIK F  DLPEQVSEAELI+K+HELNANPEVHGILVQL LP HINEEKVL+EI
Sbjct: 61  RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPKAI NGCLELLSRSGISIRGKKAVV+GRS
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKAIMNGCLELLSRSGISIRGKKAVVMGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVTIVHS SVDPESVIREADIIIAAAG AQMIKGSWIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNTLDGAKR 
Sbjct: 241 DVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRA 300

Query: 308 IEQ 311
           IEQ
Sbjct: 301 IEQ 303

[Close]

BLAST of MS019065.1 vs. ExPASy TrEMBL
Match: A0A0A0KWH1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G001540 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.6e-148
Identity = 274/310 (88.39%), Postives = 290/310 (93.55%), Query Frame = 0


Query: 1   FPLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCT 60
           F  SPAKMAS+S HKATIIDGK+IAQTVRSEI EEV+KLS+KYGK+PGLA VI+G+RK +
Sbjct: 68  FFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDS 127

Query: 61  LNYVNMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINE 120
           L YVNMKRKAC+EVGIK F  DLPEQVSEAELI+K+HELNANPEVHGILVQL LP HINE
Sbjct: 128 LTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINE 187

Query: 121 EKVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKK 180
           EKVL+EISIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPK    GC+ELLSRSGISIRGKK
Sbjct: 188 EKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPK----GCIELLSRSGISIRGKK 247

Query: 181 AVVLGRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWI 240
           AVV+GRSNIVGLPVSLLLLKADATVTIVHS SVDPESVIREADIIIAAAG AQMIKGSWI
Sbjct: 248 AVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWI 307

Query: 241 KPGAAVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNT 300
           KPGAAVIDVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNT
Sbjct: 308 KPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNT 367

Query: 301 LDGAKRVIEQ 311
           LDGAKRVIEQ
Sbjct: 368 LDGAKRVIEQ 373

[Close]

BLAST of MS019065.1 vs. ExPASy TrEMBL
Match: A0A6J1CQR5 (Methenyltetrahydrofolate cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111013297 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 7.7e-148
Identity = 273/303 (90.10%), Postives = 286/303 (94.39%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MASDSGHKAT+IDGKEIAQ VRSEIAEEV+KLSEK+GKVPGLA VI+GSRK + +YV MK
Sbjct: 1   MASDSGHKATLIDGKEIAQAVRSEIAEEVNKLSEKHGKVPGLAVVIVGSRKDSQSYVKMK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC EVGIK F FDLPEQVSEAEL+AKIHELNANPEVHGILVQL LP+HINEEKVLAEI
Sbjct: 61  RKACAEVGIKSFDFDLPEQVSEAELVAKIHELNANPEVHGILVQLPLPKHINEEKVLAEI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPK    GCLELLSRSGISIRGKKAVV+GRS
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPK----GCLELLSRSGISIRGKKAVVMGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVTIVHS SVDPESV+READI+IAAAG AQMIKGSWIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTIVHSRSVDPESVVREADIVIAAAGQAQMIKGSWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNTLDGAKRV
Sbjct: 241 DVGTNAVDDPTRKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 299

Query: 308 IEQ 311
           IEQ
Sbjct: 301 IEQ 299

[Close]

BLAST of MS019065.1 vs. ExPASy TrEMBL
Match: A0A5D3E076 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00860 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.9e-146
Identity = 271/303 (89.44%), Postives = 285/303 (94.06%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MAS+S HKATIIDGK+IAQTVRSE+AEEV+KLSEKYGKVPGLA VI+G+RK +L YVNMK
Sbjct: 1   MASESDHKATIIDGKKIAQTVRSEVAEEVNKLSEKYGKVPGLAVVIVGNRKDSLTYVNMK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC+EVGIK F  DLPEQVSEAELI+K+HELNANPEVHGILVQL LP HINEEKVL+EI
Sbjct: 61  RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SIEKD+DGFHPLNIGKLAMKGRDPLFLPCTPK    GCLELLSRSGISIRGKKAVV+GRS
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPK----GCLELLSRSGISIRGKKAVVMGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVTIVHS SVDPESVIREADIIIAAAG AQMIKGSWIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAVDDPT KSGYRLVGDVDFQEACKVAGWITPVPGGVGPM VAM+LRNTLDGAKR 
Sbjct: 241 DVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRA 299

Query: 308 IEQ 311
           IEQ
Sbjct: 301 IEQ 299

[Close]

BLAST of MS019065.1 vs. TAIR 10
Match: AT3G12290.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 458.0 bits (1177), Expect = 6.1e-129
Identity = 232/300 (77.33%), Postives = 262/300 (87.33%), Query Frame = 0


Query: 8   MASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYVNMK 67
           MAS S H A IIDGK IA T+RSEIAEEV  LSEK+GKVPGLA VI+GSRK +  YVN K
Sbjct: 1   MASSSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTK 60

Query: 68  RKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVLAEI 127
           RKAC EVGIK F   LPE+VSEA+LI+K+HELN+NP+VHGILVQL LP+HINEE +L  I
Sbjct: 61  RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120

Query: 128 SIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVLGRS 187
           SI+KD+DGFHPLNIGKLAMKGR+PLFLPCTPK    GCLELL+RSG+ I+G++AVV+GRS
Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPK----GCLELLARSGVKIKGQRAVVVGRS 180

Query: 188 NIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGAAVI 247
           NIVGLPVSLLLLKADATVT VHS + DPE++IREADI+IAA G A MIKG+WIKPGAAVI
Sbjct: 181 NIVGLPVSLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVI 240

Query: 248 DVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGAKRV 307
           DVGTNAV DP+ KSGYRLVGDVDF EA KVAG+ITPVPGGVGPM VAM+LRNT+DGAKRV
Sbjct: 241 DVGTNAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKRV 296

[Close]

BLAST of MS019065.1 vs. TAIR 10
Match: AT4G00620.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 351.7 bits (901), Expect = 6.1e-97
Identity = 173/306 (56.54%), Postives = 225/306 (73.53%), Query Frame = 0


Query: 2   PLSPAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTL 61
           P+S     + S   A +IDGK +A+ +R EI  EVS++ E  G +PGLA +++G RK + 
Sbjct: 55  PISSISADTKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSA 114

Query: 62  NYVNMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEE 121
            YV  K+KAC  VGIK F   L E  SE E++  +   N +P VHGILVQL LP H++E+
Sbjct: 115 TYVRNKKKACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQ 174

Query: 122 KVLAEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKA 181
            +L  +SIEKD+DGFHPLNIG+LAM+GR+PLF+PCTPK    GC+ELL R  I I+GK+A
Sbjct: 175 NILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPK----GCIELLHRYNIEIKGKRA 234

Query: 182 VVLGRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIK 241
           VV+GRSNIVG+P +LLL + DATV+I+HS + +PE + READIII+A G   M++GSWIK
Sbjct: 235 VVIGRSNIVGMPAALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIK 294

Query: 242 PGAAVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTL 301
           PGA +IDVG N V+DP+   GYRLVGD+ ++EA KVA  ITPVPGGVGPM +AM+L NTL
Sbjct: 295 PGAVLIDVGINPVEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTL 354

Query: 302 DGAKRV 308
             AKR+
Sbjct: 355 TSAKRI 356

[Close]

BLAST of MS019065.1 vs. TAIR 10
Match: AT2G38660.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.4e-93
Identity = 164/303 (54.13%), Postives = 225/303 (74.26%), Query Frame = 0


Query: 5   PAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYV 64
           P  ++ ++  K  +IDG  IA+ +R++I  EV K+ +  GKVPGLA V++G ++ +  YV
Sbjct: 52  PPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYV 111

Query: 65  NMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVL 124
             K KAC E GIK    +LPE  +E ++I+ + + N +  +HGILVQL LP+H+NE K+L
Sbjct: 112 RNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKIL 171

Query: 125 AEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVL 184
             + +EKD+DGFHPLN+G LAM+GR+PLF+ CTPK    GC+ELL R+G+ I GK AVV+
Sbjct: 172 NMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPK----GCVELLIRTGVEIAGKNAVVI 231

Query: 185 GRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGA 244
           GRSNIVGLP+SLLL + DATV+ VH+ + DPE + R+ADI+IAAAG+  +++GSW+KPGA
Sbjct: 232 GRSNIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGA 291

Query: 245 AVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGA 304
            VIDVGT  V+D + + GYRLVGDV ++EA  VA  ITPVPGGVGPM + M+L NTL+ A
Sbjct: 292 VVIDVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAA 350

Query: 305 KRV 308
           KR+
Sbjct: 352 KRI 350

[Close]

BLAST of MS019065.1 vs. TAIR 10
Match: AT2G38660.3 (Amino acid dehydrogenase family protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.4e-93
Identity = 164/303 (54.13%), Postives = 225/303 (74.26%), Query Frame = 0


Query: 5   PAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYV 64
           P  ++ ++  K  +IDG  IA+ +R++I  EV K+ +  GKVPGLA V++G ++ +  YV
Sbjct: 52  PPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYV 111

Query: 65  NMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVL 124
             K KAC E GIK    +LPE  +E ++I+ + + N +  +HGILVQL LP+H+NE K+L
Sbjct: 112 RNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKIL 171

Query: 125 AEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVL 184
             + +EKD+DGFHPLN+G LAM+GR+PLF+ CTPK    GC+ELL R+G+ I GK AVV+
Sbjct: 172 NMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPK----GCVELLIRTGVEIAGKNAVVI 231

Query: 185 GRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGA 244
           GRSNIVGLP+SLLL + DATV+ VH+ + DPE + R+ADI+IAAAG+  +++GSW+KPGA
Sbjct: 232 GRSNIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGA 291

Query: 245 AVIDVGTNAVDDPTTKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMAVAMMLRNTLDGA 304
            VIDVGT  V+D + + GYRLVGDV ++EA  VA  ITPVPGGVGPM + M+L NTL+ A
Sbjct: 292 VVIDVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAA 350

Query: 305 KRV 308
           KR+
Sbjct: 352 KRI 350

[Close]

BLAST of MS019065.1 vs. TAIR 10
Match: AT2G38660.4 (Amino acid dehydrogenase family protein )

HSP 1 Score: 298.1 bits (762), Expect = 8.1e-81
Identity = 143/270 (52.96%), Postives = 200/270 (74.07%), Query Frame = 0


Query: 5   PAKMASDSGHKATIIDGKEIAQTVRSEIAEEVSKLSEKYGKVPGLAEVIIGSRKCTLNYV 64
           P  ++ ++  K  +IDG  IA+ +R++I  EV K+ +  GKVPGLA V++G ++ +  YV
Sbjct: 52  PPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYV 111

Query: 65  NMKRKACVEVGIKYFGFDLPEQVSEAELIAKIHELNANPEVHGILVQLRLPRHINEEKVL 124
             K KAC E GIK    +LPE  +E ++I+ + + N +  +HGILVQL LP+H+NE K+L
Sbjct: 112 RNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKIL 171

Query: 125 AEISIEKDIDGFHPLNIGKLAMKGRDPLFLPCTPKAITNGCLELLSRSGISIRGKKAVVL 184
             + +EKD+DGFHPLN+G LAM+GR+PLF+ CTPK    GC+ELL R+G+ I GK AVV+
Sbjct: 172 NMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPK----GCVELLIRTGVEIAGKNAVVI 231

Query: 185 GRSNIVGLPVSLLLLKADATVTIVHSLSVDPESVIREADIIIAAAGLAQMIKGSWIKPGA 244
           GRSNIVGLP+SLLL + DATV+ VH+ + DPE + R+ADI+IAAAG+  +++GSW+KPGA
Sbjct: 232 GRSNIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGA 291

Query: 245 AVIDVGTNAVDDPTTKSGYRLVGDVDFQEA 275
            VIDVGT  V+D + + GYRLVGDV ++EA
Sbjct: 292 VVIDVGTTPVEDSSCEFGYRLVGDVCYEEA 317

The following BLAST results are available for this feature:

BLAST of MS019065.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143448.1	5.1e-162	96.13	bifunctional protein FolD 2-like [Momordica charantia]	[more]
KAA0044358.1	1.7e-149	90.43	bifunctional protein FolD 2 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_004152261.2	3.2e-148	88.39	bifunctional protein FolD 2 [Cucumis sativus]	[more]
XP_022143417.1	1.6e-147	90.10	bifunctional protein FolD 2-like [Momordica charantia]	[more]
XP_008454369.1	3.9e-146	89.44	PREDICTED: bifunctional protein FolD 2 isoform X1 [Cucumis melo] >TYK29487.1 bif...	[more]

BLAST of MS019065.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHH7	8.6e-128	77.33	Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1	[more]
O65271	8.6e-96	56.54	Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO...	[more]
A2RVV7	3.4e-92	54.13	Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FO...	[more]
O65269	2.9e-75	49.33	Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO...	[more]
A0LE04	1.6e-73	51.89	Bifunctional protein FolD OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 1...	[more]

BLAST of MS019065.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQR1	2.5e-162	96.13	bifunctional protein FolD 2-like OS=Momordica charantia OX=3673 GN=LOC111013324 ...	[more]
A0A5A7TNB3	8.3e-150	90.43	Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A0A0KWH1	1.6e-148	88.39	Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G0015...	[more]
A0A6J1CQR5	7.7e-148	90.10	Methenyltetrahydrofolate cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3E076	1.9e-146	89.44	Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]

BLAST of MS019065.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12290.1	6.1e-129	77.33	Amino acid dehydrogenase family protein	[more]
AT4G00620.1	6.1e-97	56.54	Amino acid dehydrogenase family protein	[more]
AT2G38660.1	2.4e-93	54.13	Amino acid dehydrogenase family protein	[more]
AT2G38660.3	2.4e-93	54.13	Amino acid dehydrogenase family protein	[more]
AT2G38660.4	8.1e-81	52.96	Amino acid dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000672	Tetrahydrofolate dehydrogenase/cyclohydrolase	PRINTS	PR00085	THFDHDRGNASE	coord: 174..194 score: 56.78 coord: 281..299 score: 74.7 coord: 47..69 score: 39.97 coord: 88..115 score: 50.69 coord: 123..144 score: 51.05 coord: 264..280 score: 48.64 coord: 223..252 score: 52.31
IPR000672	Tetrahydrofolate dehydrogenase/cyclohydrolase	HAMAP	MF_01576	THF_DHG_CYH	coord: 16..308 score: 34.614689
IPR020630	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain	PFAM	PF00763	THF_DHG_CYH	coord: 18..134 e-value: 3.0E-35 score: 121.0
None	No IPR available	GENE3D	3.40.50.10860	Leucine Dehydrogenase, chain A, domain 1	coord: 21..301 e-value: 6.0E-103 score: 345.5
None	No IPR available	GENE3D	3.40.50.720		coord: 19..284 e-value: 6.0E-103 score: 345.5
None	No IPR available	PANTHER	PTHR48099	C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED	coord: 9..308
None	No IPR available	PANTHER	PTHR48099:SF10	BIFUNCTIONAL PROTEIN FOLD 1, MITOCHONDRIAL	coord: 9..308
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 16..135
IPR020631	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain	PFAM	PF02882	THF_DHG_CYH_C	coord: 137..306 e-value: 5.7E-61 score: 204.5
IPR020631	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain	CDD	cd01080	NAD_bind_m-THF_DH_Cyclohyd	coord: 129..305 e-value: 6.70669E-86 score: 253.247
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 136..303

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS019065	MS019065	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS019065.1-cds	MS019065.1-cds-scaffold20:1151888..1152022	CDS
MS019065.1-cds	MS019065.1-cds-scaffold20:1152131..1152314	CDS
MS019065.1-cds	MS019065.1-cds-scaffold20:1152718..1152887	CDS
MS019065.1-cds	MS019065.1-cds-scaffold20:1153078..1153290	CDS
MS019065.1-cds	MS019065.1-cds-scaffold20:1154075..1154302	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS019065.1	MS019065.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004488	methylenetetrahydrofolate dehydrogenase (NADP+) activity