MS019033.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS019033.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Epoxide hydrolase 2
Location	scaffold20: 846235 .. 847750 (-)
Sequence length	969
RNA-Seq Expression	MS019033.1
Synteny	MS019033.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAGAACGAGAGAAGCTGCCATGGACGCGATTGAGCACACCACCGTCAACACCAATGGCATCAAAATGCACATCGCCTCCATCGGCACAGGCCCCGTCGTCCTTCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTTCTGTACCTTTCCTCCGTCGGATACCGCGCCATCGCGCCCGATCTCCGCGGCTACGGCGACACGGACTCGCCGGAATCGCATACGTCATACTCTGCGCTCCACATCGTCGGCGATTTGGTTGGGGCTCTGGACGGGCTCGGGATCGACAAGGTGTTCCTGGTAGGGCACGATTGGGGGGCGATAATCGCCTGGTACTTCTGCTTGTTCAGGCCAGATCGGATCAAGGCGCTGGTGAATCTCAGCGTCCATTTCTTACCTAGGAACCCTGCGATTTCGTTTGTCGAGGGTTTCAGAGCTGCGTATGGCGATGACTTCTACATTTGCAGGTTTCAGGTGAATTTGTGAATTTTGTTGTGCTTTTCTTCAATTCGTAAGAAGTAATTGAAATTTGAGCTGCTAATTAAACTGTGGTATTCTGCTGTCCTGCTCTTTCTTGGTAAGTAGATTGAAAAGTTTCCTGAGTTAGGAGTTGCGCGTTAGAAGTTGCAAATGAGTGTAGCAACGTTGCTAATTAGAGTCCTATTTGGCTGATTCTCAAAGTCTTTGCAATGAACTGAAAGTTTCATATAAACACAGAAAACAAAAATGAATTGATTGTGACATGATAGATGGCTCATTGGTCTAGGGAAGAGAAAATGAGTTTAAATTCTATGATGGTCACTTAATTGACGAATTATCACGAATTTCAAAAAAAATCTATATGGGATTAATTTTCAAGTTTCTAACAGGCTCCAGGAGAGGCAGAGCAACATTTTACCTCGGTTGACACAGCTCTGGTTTTGAAGGCATTGTTAAGTAATAGAAGTACAGATCCTCCAAAGTTGCCTAAAGAAGTTGGATTCCGTGGGAGACCACCTCCAGAAAACCTTCCTTCTTGGCTGACAGAAGAAGACATCAACTATTTTGCTGCCAAATTTAAGCACACAGGCTTTACTGGAGGATTGAACTACTATCGAGCTTTCGACCTGTAAGCCGTAGTTGCATCTGATTGACTCTGATCGAACACGTCCCGCGTTTGAAGTTAGAAAAAAAATGGCTCCATATTTTCAAACTCAGAACATGTTTTGTTGATTCAAATGGTTGTTTGTATATATGAAAGTGAGTTGACGTTTGTTTCAGAACTTGGGAGCTGACCGCGCCATGGACGGGAGCACAGATCCAGGTGCCGGTGAAGTTCGTCGTCGGGGATTTGGATATGACCTATCATTTTGCAGGAGCCAAGGAATACATCCACAACGGCGGATTCAAAAAGGATGTGCCGTTTCTGGAGGAAGTGGTGGTAATGAAAGATACTGCTCACTTCATCAACCAAGAAAGGCCACAGGAAATCAGTACTCACATCCATGACTTCATCAAGAAATTC

mRNA sequence

GAGAGAACGAGAGAAGCTGCCATGGACGCGATTGAGCACACCACCGTCAACACCAATGGCATCAAAATGCACATCGCCTCCATCGGCACAGGCCCCGTCGTCCTTCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTTCTGTACCTTTCCTCCGTCGGATACCGCGCCATCGCGCCCGATCTCCGCGGCTACGGCGACACGGACTCGCCGGAATCGCATACGTCATACTCTGCGCTCCACATCGTCGGCGATTTGGTTGGGGCTCTGGACGGGCTCGGGATCGACAAGGTGTTCCTGGTAGGGCACGATTGGGGGGCGATAATCGCCTGGTACTTCTGCTTGTTCAGGCCAGATCGGATCAAGGCGCTGGTGAATCTCAGCGTCCATTTCTTACCTAGGAACCCTGCGATTTCGTTTGTCGAGGGTTTCAGAGCTGCGTATGGCGATGACTTCTACATTTGCAGGTTTCAGGCTCCAGGAGAGGCAGAGCAACATTTTACCTCGGTTGACACAGCTCTGGTTTTGAAGGCATTGTTAAGTAATAGAAGTACAGATCCTCCAAAGTTGCCTAAAGAAGTTGGATTCCGTGGGAGACCACCTCCAGAAAACCTTCCTTCTTGGCTGACAGAAGAAGACATCAACTATTTTGCTGCCAAATTTAAGCACACAGGCTTTACTGGAGGATTGAACTACTATCGAGCTTTCGACCTAACTTGGGAGCTGACCGCGCCATGGACGGGAGCACAGATCCAGGTGCCGGTGAAGTTCGTCGTCGGGGATTTGGATATGACCTATCATTTTGCAGGAGCCAAGGAATACATCCACAACGGCGGATTCAAAAAGGATGTGCCGTTTCTGGAGGAAGTGGTGGTAATGAAAGATACTGCTCACTTCATCAACCAAGAAAGGCCACAGGAAATCAGTACTCACATCCATGACTTCATCAAGAAATTC

Coding sequence (CDS)

GAGAGAACGAGAGAAGCTGCCATGGACGCGATTGAGCACACCACCGTCAACACCAATGGCATCAAAATGCACATCGCCTCCATCGGCACAGGCCCCGTCGTCCTTCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTTCTGTACCTTTCCTCCGTCGGATACCGCGCCATCGCGCCCGATCTCCGCGGCTACGGCGACACGGACTCGCCGGAATCGCATACGTCATACTCTGCGCTCCACATCGTCGGCGATTTGGTTGGGGCTCTGGACGGGCTCGGGATCGACAAGGTGTTCCTGGTAGGGCACGATTGGGGGGCGATAATCGCCTGGTACTTCTGCTTGTTCAGGCCAGATCGGATCAAGGCGCTGGTGAATCTCAGCGTCCATTTCTTACCTAGGAACCCTGCGATTTCGTTTGTCGAGGGTTTCAGAGCTGCGTATGGCGATGACTTCTACATTTGCAGGTTTCAGGCTCCAGGAGAGGCAGAGCAACATTTTACCTCGGTTGACACAGCTCTGGTTTTGAAGGCATTGTTAAGTAATAGAAGTACAGATCCTCCAAAGTTGCCTAAAGAAGTTGGATTCCGTGGGAGACCACCTCCAGAAAACCTTCCTTCTTGGCTGACAGAAGAAGACATCAACTATTTTGCTGCCAAATTTAAGCACACAGGCTTTACTGGAGGATTGAACTACTATCGAGCTTTCGACCTAACTTGGGAGCTGACCGCGCCATGGACGGGAGCACAGATCCAGGTGCCGGTGAAGTTCGTCGTCGGGGATTTGGATATGACCTATCATTTTGCAGGAGCCAAGGAATACATCCACAACGGCGGATTCAAAAAGGATGTGCCGTTTCTGGAGGAAGTGGTGGTAATGAAAGATACTGCTCACTTCATCAACCAAGAAAGGCCACAGGAAATCAGTACTCACATCCATGACTTCATCAAGAAATTC

Protein sequence

ERTREAAMDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNRSTDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELTAPWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQERPQEISTHIHDFIKKF

Homology

[Close]

BLAST of MS019033.1 vs. NCBI nr
Match: XP_022155318.1 (uncharacterized protein LOC111022450 [Momordica charantia])

HSP 1 Score: 668.7 bits (1724), Expect = 2.5e-188
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR
Sbjct: 121 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
           STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT
Sbjct: 181 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE
Sbjct: 241 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RPQEISTHIHDFIKKF
Sbjct: 301 RPQEISTHIHDFIKKF 316

[Close]

BLAST of MS019033.1 vs. NCBI nr
Match: XP_022934345.1 (uncharacterized protein LOC111441538 [Cucurbita moschata] >KAG6581272.1 hypothetical protein SDJN03_21274, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017999.1 ephA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 585.5 bits (1508), Expect = 2.8e-163
Identity = 270/316 (85.44%), Postives = 290/316 (91.77%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDT+SPESHTSY+ LHIVGDLVGALD LGI+KVF+VGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTESPESHTSYTVLHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F+PRNPAI FVEGFR+A+GDDFYICRFQ PGEAE+ F S+DTA + K  L +R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
            +DPPKLPK VG R  P PENLPSWLTE+DINYFA  FK TGFTGGLNYYRAFDLTWELT
Sbjct: 181 RSDPPKLPKGVGVRSIPSPENLPSWLTEKDINYFATTFKRTGFTGGLNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           A WT AQ++VPVKF++GDLD+TY F GAKEYIHNGGFKKDVPFLEEV+VMKDTAHFINQE
Sbjct: 241 ASWTKAQVRVPVKFIIGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVIVMKDTAHFINQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

[Close]

BLAST of MS019033.1 vs. NCBI nr
Match: XP_023528763.1 (uncharacterized protein LOC111791601 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.3 bits (1505), Expect = 6.3e-163
Identity = 271/316 (85.76%), Postives = 288/316 (91.14%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDT+SPESHTSY+ LHIVGDLVGALD LGI+KVFLVGHDWGAIIAWY CLFRPDRIKA
Sbjct: 61  GYGDTESPESHTSYTVLHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYLCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F+PRNPAI FVEGFR+A+GDDFYICRFQ PGEAE+ F S+DTA + K  L +R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
            +DPPKLPK VG R  P PENLPSWLTE DINYFA  FK TGFTGGLNYYRAFDLTWELT
Sbjct: 181 RSDPPKLPKGVGVRSIPSPENLPSWLTENDINYFATTFKRTGFTGGLNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           A WT AQ++VPVKF+VGDLD+TY F GAKEYIHNGGFKKDVPFLEEV+VMKDTAHFINQE
Sbjct: 241 ASWTKAQVRVPVKFIVGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVIVMKDTAHFINQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

[Close]

BLAST of MS019033.1 vs. NCBI nr
Match: XP_022984060.1 (uncharacterized protein LOC111482484 [Cucurbita maxima])

HSP 1 Score: 580.5 bits (1495), Expect = 9.1e-162
Identity = 269/316 (85.13%), Postives = 288/316 (91.14%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDT+SPE HTSY+ LHIVGDLVGALD LGI+KVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTESPELHTSYTVLHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F+PRNPAI FVEGFR+A+GDDFYICRFQ PGEAE+ F S+DTA + K  L +R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
            +DPP+LPK VG R  P PE LPSWLTEEDINYFA  FK TGFTGGLNYYRAFDLTWELT
Sbjct: 181 RSDPPELPKGVGVRSIPSPEKLPSWLTEEDINYFATIFKRTGFTGGLNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           A WT AQ++VPVKF+VGDLD+TY F GAKEYIHNGGFKKDVPFLEEV++MKDTAHFINQE
Sbjct: 241 ASWTKAQVRVPVKFIVGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVILMKDTAHFINQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

[Close]

BLAST of MS019033.1 vs. NCBI nr
Match: XP_022984062.1 (uncharacterized protein LOC111482485 [Cucurbita maxima])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 267/316 (84.49%), Postives = 287/316 (90.82%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDTDSPESHTSY+ALHIVGDLVGALD LGI+KVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F PRNP ISF+E FRA YGDDFY+CRFQ PGEAE  F  +DTA + K+ LSNR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
           ST  P LPKE GFR  PPPENLPSWLTEEDIN++A+KFK TGFTG LNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
            PWTGAQIQVPVKF+VGDLD+TYHF GAKEYIHNGGFK+DVP LEEVV+++D  HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EI+THIHDFI KF
Sbjct: 301 RPHEINTHIHDFINKF 316

[Close]

BLAST of MS019033.1 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-56
Identity = 120/328 (36.59%), Postives = 179/328 (54.57%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGT--GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 67
           M A     V+TNG+++ +   G    PVV+L HGFPEL YSWRHQ+  L+  GY  +APD
Sbjct: 1   MGAPTERLVDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPD 60

Query: 68  LRGYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRI 127
            RGYG +  PE+  +Y    +  DLVG LD +G ++   VGHDWGA++ W   L   DR+
Sbjct: 61  QRGYGGSSRPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRV 120

Query: 128 KALVNLSVHFLPRNPAISFVEGFRAAYGDD-FYICRFQAPGEAEQHFTSVDTALVLKALL 187
            A+  LSV  LPR   +   + FR+ +G++ FYI  FQ PG A+      D A  ++ ++
Sbjct: 121 AAVAALSVPALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNG-DPARTMRRMI 180

Query: 188 SNRSTDPP---------KLPKEVGFRGR-PPPENLPSWLTEEDINYFAAKFKHTGFTGGL 247
                 PP           P   GF  R P P  LP+W+++E+++++  +F  TGFTGGL
Sbjct: 181 G--GLRPPGDQSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGL 240

Query: 248 NYYRAFDLTWELTAPWTGAQIQVPVKFVVGDLD--MTYHFAGAKEYIHNGGFKKDVPFLE 307
           N+YR FD  WE TA   G  I VP  F+ G  D  +T+        + +G +++      
Sbjct: 241 NWYRNFDRNWETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISGPYRE------ 300

Query: 308 EVVVMKDTAHFINQERPQEISTHIHDFI 321
             V++    H++ QERP E++  + +F+
Sbjct: 301 --VLIDGAGHWLQQERPGEVTAALLEFL 316

[Close]

BLAST of MS019033.1 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.0e-46
Identity = 95/305 (31.15%), Postives = 166/305 (54.43%), Query Frame = 0


Query: 20  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 79
           G+++H   +G+GP V L HGFPE W+SWR+Q+  L+  G+R +A D++GYG++ +P    
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 80  SYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVHFLPRN 139
            YS   +  D+V  L+ LG+ +   +GHDWG ++ W   LF P+R++A+ +L+  F+P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 140 PAISFVEGFRAAYGDDFYICRFQAPGEAEQHF-TSVDTALVLKALLSNRSTDPPKLPKEV 199
           P +S +E  +A    D+ +  FQ PG AE     ++D          + +       +E+
Sbjct: 367 PNVSPMEIIKANPVFDYQL-YFQEPGVAEAELEQNLDRTFKNFFRAHDETFLTTNRVREL 426

Query: 200 G--FRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELTAPWTGAQIQ 259
           G  F G P   +L   +TEEDI ++  +FK +GF G LN+YR  +  W+     +G +I 
Sbjct: 427 GGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRKIL 486

Query: 260 VPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQERPQEISTHI 319
           +P   V  + D+  H   +K        +  +P L+   + KD  H+   ++P E++  +
Sbjct: 487 IPALMVTAENDLVLHPKMSKH------MENWIPHLKRGHI-KDCGHWTQIDKPAELNRIL 543

Query: 320 HDFIK 322
            ++++
Sbjct: 547 IEWLE 543

[Close]

BLAST of MS019033.1 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 9.4e-45
Identity = 96/305 (31.48%), Postives = 158/305 (51.80%), Query Frame = 0


Query: 21  IKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHTS 80
           +++H   +G+GP V L HGFPE WYSWR+Q+  L+  GYR +A D++GYG++ +P     
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 81  YSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVHFLPRNP 140
           Y    +  ++V  LD LG+ +   +GHDWG ++ WY  LF P+R++A+ +L+  F+P NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 141 AISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNRSTDPPKLPK---- 200
            +S +E  +A    D+ +  FQ PG AE      + +   K+L   R++D   L      
Sbjct: 368 NMSPLESIKANPVFDYQL-YFQEPGVAEAELEQ-NLSRTFKSLF--RASDESVLSMHKVC 427

Query: 201 EVG--FRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELTAPWTGAQ 260
           E G  F   P   +L   +TEE+I ++  +FK +GF G LN+YR  +  W+      G +
Sbjct: 428 EAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRK 487

Query: 261 IQVPVKFVVGDLD------MTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQER 314
           I +P   V  + D      M+ H      ++  G              ++D  H+   ++
Sbjct: 488 ILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGH-------------IEDCGHWTQMDK 535

[Close]

BLAST of MS019033.1 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-43
Identity = 94/298 (31.54%), Postives = 156/298 (52.35%), Query Frame = 0


Query: 20  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 79
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 80  SYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVHFLPRN 139
            Y+   +  ++V  LD LGI +   +GHDW  ++ W   LF P+R++A+ +L+  F+P +
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 140 PAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNRSTDPPKLPKEVG 199
           P +S ++  R+    ++ +  FQ PG AE       +        ++  T    + K   
Sbjct: 365 PDVSPMKVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRASDETGFIAVHKATE 424

Query: 200 FRG----RPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELTAPWTGAQI 259
             G     P   NL    TEE+I ++  +FK TGF G LN+YR  +  W+ +    G +I
Sbjct: 425 IGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRKI 484

Query: 260 QVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQERPQEIS 314
            VP   V  + D+      +K        +K +PFL+   + +D  H+   E+P E++
Sbjct: 485 LVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKRGHI-EDCGHWTQIEKPTEVN 534

[Close]

BLAST of MS019033.1 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.2e-43
Identity = 97/315 (30.79%), Postives = 162/315 (51.43%), Query Frame = 0


Query: 20  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 79
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 80  SYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVHFLPRN 139
            Y+   +  ++V  L+ LGI +   +GHDW  ++ W   LF P+R++A+ +L+   +P N
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 140 PAISFVEGFRAAYGDDFYICRFQAPGEAEQHF-------------TSVDTALVLKALLSN 199
           P +S +E  R+    ++ +  FQ PG AE                TS D  L    L  N
Sbjct: 365 PEVSPMEVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRTSDDMGL----LTVN 424

Query: 200 RSTDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWEL 259
           ++T+   +       G P    +    TEE+I Y+  +FK +GF G LN+YR  +  W+ 
Sbjct: 425 KATEMGGI-----LVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKW 484

Query: 260 TAPWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQ 319
           +    G +I VP   V  + D+      +K        +  +PFL+   + +D  H+   
Sbjct: 485 SCKALGRKILVPALMVTAEKDIVLRPEMSK------NMENWIPFLKRGHI-EDCGHWTQI 542

Query: 320 ERPQEISTHIHDFIK 322
           E+P E++  +  ++K
Sbjct: 545 EKPAEVNQILIKWLK 542

[Close]

BLAST of MS019033.1 vs. ExPASy TrEMBL
Match: A0A6J1DMN0 (uncharacterized protein LOC111022450 OS=Momordica charantia OX=3673 GN=LOC111022450 PE=4 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.2e-188
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR
Sbjct: 121 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
           STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT
Sbjct: 181 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE
Sbjct: 241 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RPQEISTHIHDFIKKF
Sbjct: 301 RPQEISTHIHDFIKKF 316

[Close]

BLAST of MS019033.1 vs. ExPASy TrEMBL
Match: A0A6J1F7F3 (uncharacterized protein LOC111441538 OS=Cucurbita moschata OX=3662 GN=LOC111441538 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 270/316 (85.44%), Postives = 290/316 (91.77%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDT+SPESHTSY+ LHIVGDLVGALD LGI+KVF+VGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTESPESHTSYTVLHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F+PRNPAI FVEGFR+A+GDDFYICRFQ PGEAE+ F S+DTA + K  L +R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
            +DPPKLPK VG R  P PENLPSWLTE+DINYFA  FK TGFTGGLNYYRAFDLTWELT
Sbjct: 181 RSDPPKLPKGVGVRSIPSPENLPSWLTEKDINYFATTFKRTGFTGGLNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           A WT AQ++VPVKF++GDLD+TY F GAKEYIHNGGFKKDVPFLEEV+VMKDTAHFINQE
Sbjct: 241 ASWTKAQVRVPVKFIIGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVIVMKDTAHFINQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

[Close]

BLAST of MS019033.1 vs. ExPASy TrEMBL
Match: A0A6J1J7M9 (uncharacterized protein LOC111482484 OS=Cucurbita maxima OX=3661 GN=LOC111482484 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.4e-162
Identity = 269/316 (85.13%), Postives = 288/316 (91.14%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDT+SPE HTSY+ LHIVGDLVGALD LGI+KVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTESPELHTSYTVLHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F+PRNPAI FVEGFR+A+GDDFYICRFQ PGEAE+ F S+DTA + K  L +R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
            +DPP+LPK VG R  P PE LPSWLTEEDINYFA  FK TGFTGGLNYYRAFDLTWELT
Sbjct: 181 RSDPPELPKGVGVRSIPSPEKLPSWLTEEDINYFATIFKRTGFTGGLNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           A WT AQ++VPVKF+VGDLD+TY F GAKEYIHNGGFKKDVPFLEEV++MKDTAHFINQE
Sbjct: 241 ASWTKAQVRVPVKFIVGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVILMKDTAHFINQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

[Close]

BLAST of MS019033.1 vs. ExPASy TrEMBL
Match: A0A6J1J139 (uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.5e-162
Identity = 267/316 (84.49%), Postives = 287/316 (90.82%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDTDSPESHTSY+ALHIVGDLVGALD LGI+KVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F PRNP ISF+E FRA YGDDFY+CRFQ PGEAE  F  +DTA + K+ LSNR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
           ST  P LPKE GFR  PPPENLPSWLTEEDIN++A+KFK TGFTG LNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
            PWTGAQIQVPVKF+VGDLD+TYHF GAKEYIHNGGFK+DVP LEEVV+++D  HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EI+THIHDFI KF
Sbjct: 301 RPHEINTHIHDFINKF 316

[Close]

BLAST of MS019033.1 vs. ExPASy TrEMBL
Match: A0A6J1F1J8 (uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC111441539 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 264/316 (83.54%), Postives = 285/316 (90.19%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGDTDSPESHTSY+ALHIVGDLVGALD LGI+KVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVNLSV F PRNP ISF+E FRA YGDDFY+CRFQ PGEAE  F  +DTA + K  LSNR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
           ST  P LPKE GFR  PPPE+LPSWLTEEDIN++A+KFK TGFTG LNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPESLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
            PWTGAQIQVPVKF+VGDLD+TYHF GAKEYIHNGGFK+DVP LEEVV+++D  HF+++E
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 308 RPQEISTHIHDFIKKF 324
           RP EI+THIHDF  KF
Sbjct: 301 RPHEINTHIHDFFNKF 316

[Close]

BLAST of MS019033.1 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 470.3 bits (1209), Expect = 1.2e-132
Identity = 203/316 (64.24%), Postives = 256/316 (81.01%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 67
           M+ IEHTT++TNGI MH+ASIG+GPV+L +HGFP+LWYSWRHQL+  +++GYRAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 68  GYGDTDSPESHTSYSALHIVGDLVGALDGLGIDKVFLVGHDWGAIIAWYFCLFRPDRIKA 127
           GYGD+D+P S  SY+ LHIVGDLVG LD LG+D+VFLVGHDWGAI+AW+ C+ RPDR+ A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 128 LVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALLSNR 187
           LVN SV F PRNP++  V+ FRA +GDD+YICRFQ PGE E+ F  VDT  ++    ++R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 188 STDPPKLPKEVGFRGRPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLTWELT 247
           +  PP +PK VGFRG P P +LP+WLTE+D+ ++  KF   GFTGGLNYYRA +L+WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 248 APWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHFINQE 307
           APWTG QI+VPVKF+VGDLD+TY+  G KEYIH GG KK VPFL+EVVVM+   HF++QE
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 308 RPQEISTHIHDFIKKF 324
           +P E++ HI+ F KKF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

[Close]

BLAST of MS019033.1 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 411.0 bits (1055), Expect = 8.9e-115
Identity = 187/323 (57.89%), Postives = 239/323 (73.99%), Query Frame = 0


Query: 8   MDAIEHTTVNTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 67
           M+ I+H  V+ NGI MHIA  G   GPVVLLLHGFP+LWY+WRHQ+  LSS+GYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 68  LRGYGDTDSPESHTSYSALHIVGDLVGALDGL--GIDKVFLVGHDWGAIIAWYFCLFRPD 127
           LRGYGD+DSPES + Y+ L++VGDLV  LD +    +KVFLVGHDWGAII W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 128 RIKALVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKAL 187
           +I   V LSV +  RNP +  V+GF+A +GDD+YICRFQ PG+ E    S D  + L+ L
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 188 LSNRSTDPPKLPKEVGFRGRPPPEN----LPSWLTEEDINYFAAKFKHTGFTGGLNYYRA 247
            + R+  PP LPK+  F  +P P +    LP W +++D++++ +KF+  GFTGGLNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 248 FDLTWELTAPWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKD 307
            DL WELTAPWTGA+IQVPVKF+ GD DM Y   G KEYIH GGF  DVP L+E+VV++D
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 308 TAHFINQERPQEISTHIHDFIKK 323
             HF+NQE+PQE++ HI+DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

[Close]

BLAST of MS019033.1 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 380.2 bits (975), Expect = 1.7e-105
Identity = 175/331 (52.87%), Postives = 234/331 (70.69%), Query Frame = 0


Query: 1   ERTREAAMDAIEHTTVNTNGIKMHIA-----SIGTGPVVLLLHGFPELWYSWRHQLLYLS 60
           +R  ++ +D +EH T+  NGI MH+A       G  P++L LHGFPELWY+WRHQ++ LS
Sbjct: 44  KRPEKSRLDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALS 103

Query: 61  SVGYRAIAPDLRGYGDTDSPESHTSYSALHIVGDLVGALDGL-GIDK-VFLVGHDWGAII 120
           S+GYR IAPDLRGYGDT++PE    Y+ L++ GD+V  +D + G DK V +VGHDWGA+I
Sbjct: 104 SLGYRTIAPDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMI 163

Query: 121 AWYFCLFRPDRIKALVNLSVHFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTS 180
           AW  C +RP+++KALVN+SV F PRNP    V   R  +GDD+Y+CRFQ  GE E  F  
Sbjct: 164 AWQLCQYRPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKK 223

Query: 181 VDTALVLKALLSNRSTDPPKLPKEVGF-RGRPPPENLPSWLTEEDINYFAAKFKHTGFTG 240
           + T  VLK  L+ ++  P  LPK+  F R       LP WLT+ED++Y+  K+++ GFTG
Sbjct: 224 LGTENVLKEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTG 283

Query: 241 GLNYYRAFDLTWELTAPWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLE 300
            +NYYR  D  WELTAPWTGA+I+VPVKF++GD D+TY+F GAKEYI+ GGFK+DVP L+
Sbjct: 284 PINYYRNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLD 343

Query: 301 EVVVMKDTAHFINQERPQEISTHIHDFIKKF 324
           E VV+K   HF+++E P  I+ HIH+F  KF
Sbjct: 344 ETVVLKGLGHFLHEENPDVINQHIHNFFHKF 374

[Close]

BLAST of MS019033.1 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 374.0 bits (959), Expect = 1.2e-103
Identity = 182/321 (56.70%), Postives = 228/321 (71.03%), Query Frame = 0


Query: 11  IEHTTVNTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 70
           +EH  V  NGI +H+A  G   GP+VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 71  YGDTDSPESHTSYSALHIVGDLVGALDGLGI---DKVFLVGHDWGAIIAWYFCLFRPDRI 130
           YGD+D+P   +SY+  +IVGDL+  +  L     +KVF+VGHDWGA+IAWY CLFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 131 KALVNLSV--HFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKAL 190
           KALVNLSV   F P +P++  V+  RA YGDD+YICRFQ  G+ E     V T  V+K L
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 191 LSNRSTDPPKLPKEVGFRG-RPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDL 250
           L+ R+  P  +PK+  F G +     LPSWLTEED+ YF +KF+  GF+G +NYYR F+ 
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNR 240

Query: 251 TWELTAPWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAH 310
             EL  PW G++IQVP KFV+G+LD+ Y+  G KEYIH   FK+DVP LEE VVM+  AH
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAH 300

Query: 311 FINQERPQEISTHIHDFIKKF 324
           FINQE+PQEI   I DFI KF
Sbjct: 301 FINQEKPQEILQIILDFISKF 321

[Close]

BLAST of MS019033.1 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 362.8 bits (930), Expect = 2.8e-100
Identity = 176/320 (55.00%), Postives = 225/320 (70.31%), Query Frame = 0


Query: 11  IEHTTVNTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 70
           +EH  V  NGI +H+A  G   G +VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 71  YGDTDSPESHTSYSALHIVGDLVGALDGLGID--KVFLVGHDWGAIIAWYFCLFRPDRIK 130
           YGD+D+P   +S++  +IVGDLV  +  L  +  KVF+VGHDWGA+IAWY CLFRPD++K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 131 ALVNLSV--HFLPRNPAISFVEGFRAAYGDDFYICRFQAPGEAEQHFTSVDTALVLKALL 190
           ALVNLSV   F P +P++  V+  RA YG+D+Y+CRFQ  G+ E     V T  V+K LL
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 191 SNRSTDPPKLPKEVGFRG-RPPPENLPSWLTEEDINYFAAKFKHTGFTGGLNYYRAFDLT 250
           + R+  P  +PK+  F G +     LPSWLTEED+ YF +KFK  GF G +NYYR F+  
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRN 240

Query: 251 WELTAPWTGAQIQVPVKFVVGDLDMTYHFAGAKEYIHNGGFKKDVPFLEEVVVMKDTAHF 310
            EL  PW G++IQVP KFV+G+LD+ Y+  G KEYIH   FK+DVP +EE VVM+  AHF
Sbjct: 241 NELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHF 300

Query: 311 INQERPQEISTHIHDFIKKF 324
           +NQE+PQEI   I DFI  F
Sbjct: 301 LNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:

BLAST of MS019033.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155318.1	2.5e-188	100.00	uncharacterized protein LOC111022450 [Momordica charantia]	[more]
XP_022934345.1	2.8e-163	85.44	uncharacterized protein LOC111441538 [Cucurbita moschata] >KAG6581272.1 hypothet...	[more]
XP_023528763.1	6.3e-163	85.76	uncharacterized protein LOC111791601 [Cucurbita pepo subsp. pepo]	[more]
XP_022984060.1	9.1e-162	85.13	uncharacterized protein LOC111482484 [Cucurbita maxima]	[more]
XP_022984062.1	1.6e-161	84.49	uncharacterized protein LOC111482485 [Cucurbita maxima]	[more]

BLAST of MS019033.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I6YGS0	2.4e-56	36.59	Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX...	[more]
Q6Q2C2	5.0e-46	31.15	Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1	[more]
P34913	9.4e-45	31.48	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]
P34914	1.4e-43	31.54	Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2	[more]
P80299	5.2e-43	30.79	Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=...	[more]

BLAST of MS019033.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DMN0	1.2e-188	100.00	uncharacterized protein LOC111022450 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1F7F3	1.4e-163	85.44	uncharacterized protein LOC111441538 OS=Cucurbita moschata OX=3662 GN=LOC1114415...	[more]
A0A6J1J7M9	4.4e-162	85.13	uncharacterized protein LOC111482484 OS=Cucurbita maxima OX=3661 GN=LOC111482484...	[more]
A0A6J1J139	7.5e-162	84.49	uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485...	[more]
A0A6J1F1J8	1.4e-160	83.54	uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC1114415...	[more]

BLAST of MS019033.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02340.1	1.2e-132	64.24	alpha/beta-Hydrolases superfamily protein	[more]
AT3G05600.1	8.9e-115	57.89	alpha/beta-Hydrolases superfamily protein	[more]
AT4G15960.1	1.7e-105	52.87	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26740.1	1.2e-103	56.70	soluble epoxide hydrolase	[more]
AT2G26750.1	2.8e-100	55.00	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 58..73 score: 44.61 coord: 118..131 score: 26.85 coord: 104..117 score: 33.0
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 32..149 e-value: 3.4E-21 score: 76.1
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 118..131 score: 49.35 coord: 104..117 score: 52.92 coord: 58..73 score: 60.8 coord: 257..273 score: 34.22 coord: 38..56 score: 55.26 coord: 297..319 score: 39.13
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 8..323 e-value: 5.0E-104 score: 350.5
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 2..322
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 8..323
None	No IPR available	PANTHER	PTHR43329:SF58	OS05G0273800 PROTEIN	coord: 8..323

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS019033	MS019033	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS019033.1-cds	MS019033.1-cds-scaffold20:846235..846478	CDS
MS019033.1-cds	MS019033.1-cds-scaffold20:846631..846869	CDS
MS019033.1-cds	MS019033.1-cds-scaffold20:847265..847750	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS019033.1	MS019033.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity