MS018972.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS018972.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectinesterase
Location	scaffold20: 381230 .. 384066 (+)
Sequence length	1029
RNA-Seq Expression	MS018972.1
Synteny	MS018972.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCATTATTCCAAAGGGACAATTTGGTTAATACCAACGTTGTTGTTTGCCTGTCTGTATAGTGTTTCAAATGCATTGGATTGTGAGTTGAATTATCAAAATCCATACAGAGTTGCATATAGTATTGTTGTAAACAAATTTGGGAGAGGAAACTTCAGAACAATCCAAAGTGCTATTGATTCAATTCCTTTTGGAAGTACCAAATGGATTAGAGTTCAAATTTCTGCTGGCACATATTGGTAAACCATATCATTTATTTACCTTTCTTTTCTTTATGTTTTGTAGTTAATAGTAAATTTAATATTTACATCTTGTTTCGGTATCTGAACTTTCAATAAATAATCATTTTTATACCCATCAAGTTGTCGTTTTTCAACCATCAATTTTTTTTTTAATAAATACTTATTTTTATTCAAAATTCAAATTTAAATTTGACTCATCAATGATAATCCATGATAGTACAAACGTTTTCTCCTTTTATTCTGTTGAAGTAATTAATTTGAATTTTGGTAACAGGGAAAAGGTGACAATTCCCAAAGGAAAATCATGCATATTTATAGATGGGGCTGGCAGTAGGGTCACTGAAATTCAATGGAATGACCATCAAACCACAGCTACTAGTGCTACCTTCACTTCTTTTGCAGAAAACCTTGTAGTTCGAGGCATTGCATTCAGGGTAGTATTTCTAAACTTCCGCTAAATTACAAATTTGGTTCCTGAAGTTTTTAGAAAATCGTGTTTTATTTATTTCATATGTACTTTTAAAAGATTTTCAATTAATCAACGCTTAAACTCTCACGATGGTGTATTTCCTGGTACTTTAACAAATTTTTAATAAATCACTAAACTTCAATTTGTGTCTAATAGATATATATTCTTGTTGATAAACTCGATTAGTTTAACATTTACTTGTCATGCTAGCTGTATTATTTGAGGATTACATAGGAGTTTAAGTTTTAAGAGTTTAAGCATTTAGTGGCGTAAGTGTAAAATTAAGTAAAAAATCCTAATAGATTTGAGTTAGTCTTCTCATTACCAATTGATTTTTTAGATGGAATTCCATGAATATTGATATGATAAGAGAGCCCAAAGCTCAAACCAATATTGGTTCCAAAAATAGGATCCGGCCAAAAGAGTCACGATAACGATAATGAGGAACATGTTAAGGATCTCACATGAAAAGATCAGAAATCTAACAAAAATTTATAATAAACATTGACCGCTTCCAATTGGTTTTGAGATAAACCCTATAAATGTTAACCAACAACAACTTGTTAGATAAAAAATTGAGTTCAAATCTGGATAAGCGCATCTCTAAAAGCAATAGTACCGTGTAGAAACTCATGGTGTTTCATTTAAAACCGAATGTTATGAGAGTTTTGTGAATGTTTTCGAAGTCGATGAAATAAATGGAAGAGTTCATCAACCAAAATAGGATTTAAACTTATCGAAACCGTGTTTTGTGAATGAAAATGGCAGAATACTTACAATGCACCAGGAAGTGTGAGGAGAAAAGAAGATATAGCACCAGCAGTTGCAGCTCTTATAATGGGAGACAAAGCAAGTTTCTATGAGTGTAGATTTATTGGACTGCAGGACACTCTGTGGGATGGACCTGGCCGTCATCATTTCGTCAATTGCTACATCGAAGGTGTAATCGATGTCATTTCTGGTTCTGGCCAATCCATCTACGAGGTTACCTTTAAAATAATTAGTTTTAAATATTACATCATTCGAAATATATTCACGTCCGTCTTTTTATTTCTCATGGTTTTACTTGGTTTCACAAAAAATATAGTACCAACAGAGATGTTGTCCTCACTTATATAGTCGGTGTGATTTTGATTGCACACCCAATAAAAAACCAATCTAAACCCAAATCTCTTACTCCCATTATAAATTTTCTCTTTTTCCTTTTTTTCTTTTTTCACAATAGTGCAAGATTTTTTTCTTCTTGAGTTCAACGAGTGAGAATGGGTCAAAGATTCAAATTCATGACCTCTTTGATTAAAGATAGGTGCTAATTATTGTTGAAGTAGACTTATGTTGGCAGATAAAGGGTGCAAGTTGAAACCATACTTAGTCGCTTCAAATAATCATAATAGCTTGTCAGTTTCGTTATTGAGACAATAAAATAAAAAACAGTCGATCAGTATGATTTTATAGCAAAGTTTCGGCGTCGACTTTTAATTTCTCTTAAATCGATATTTTGGTTCAATTCAAGGCGTTGGTTTTGGGCAAAAACAACCTGTGGACTGAATTGCTTGTGGTTAGAGATTGGTGTTGATTTTCAAATTTTGATTTTGTATGTGTGTTTGTTTTATGGCAGAAATGTATGATAAATGTGCCAATCCATCAATATGCTCCAATATTGAGTTATGGATACATAACAGCACAAGGAAAAGAGAATCCAAATGAAGGCAGTGGCTTTGTTTTCAATGCATGCTCAGTTGTTGGAAATGGGAGAGTTTATCTTGGAAGGGCTTATAGGCCTTTCTCCACTGTCATTCTCCATAATTCTTTTCTAGCAGGTTTTATTGACCCTGCCGGCTGGAATCCTTGGGGACAAGTTGGCCATGAGTAAGTCCTGTTTTTTCTAGGATCTCCACAATACGAACGGACGTTATCAAACGGACTCTAACTTATGATTTTGTTTGTATAAATGATTTTGTAGGACGAGTTTAACATATGCAGAGCTGAATTGTCGTGGACCCGGAGCAAACATTTCGAGGCGTGTGCCTTGGCTTAAGAGACTCCATAGAGCTGAGATTAGACATTTCACAGACATTTCTTTTATCGATCAAGAAGGTTGGACTTCAAGGCTTCCTCTACTT

mRNA sequence

ATGGAGCATTATTCCAAAGGGACAATTTGGTTAATACCAACGTTGTTGTTTGCCTGTCTGTATAGTGTTTCAAATGCATTGGATTGTGAGTTGAATTATCAAAATCCATACAGAGTTGCATATAGTATTGTTGTAAACAAATTTGGGAGAGGAAACTTCAGAACAATCCAAAGTGCTATTGATTCAATTCCTTTTGGAAGTACCAAATGGATTAGAGTTCAAATTTCTGCTGGCACATATTGGGAAAAGGTGACAATTCCCAAAGGAAAATCATGCATATTTATAGATGGGGCTGGCAGTAGGGTCACTGAAATTCAATGGAATGACCATCAAACCACAGCTACTAGTGCTACCTTCACTTCTTTTGCAGAAAACCTTGTAGTTCGAGGCATTGCATTCAGGAATACTTACAATGCACCAGGAAGTGTGAGGAGAAAAGAAGATATAGCACCAGCAGTTGCAGCTCTTATAATGGGAGACAAAGCAAGTTTCTATGAGTGTAGATTTATTGGACTGCAGGACACTCTGTGGGATGGACCTGGCCGTCATCATTTCGTCAATTGCTACATCGAAGGTGTAATCGATGTCATTTCTGGTTCTGGCCAATCCATCTACGAGAAATGTATGATAAATGTGCCAATCCATCAATATGCTCCAATATTGAGTTATGGATACATAACAGCACAAGGAAAAGAGAATCCAAATGAAGGCAGTGGCTTTGTTTTCAATGCATGCTCAGTTGTTGGAAATGGGAGAGTTTATCTTGGAAGGGCTTATAGGCCTTTCTCCACTGTCATTCTCCATAATTCTTTTCTAGCAGGTTTTATTGACCCTGCCGGCTGGAATCCTTGGGGACAAGTTGGCCATGAGACGAGTTTAACATATGCAGAGCTGAATTGTCGTGGACCCGGAGCAAACATTTCGAGGCGTGTGCCTTGGCTTAAGAGACTCCATAGAGCTGAGATTAGACATTTCACAGACATTTCTTTTATCGATCAAGAAGGTTGGACTTCAAGGCTTCCTCTACTT

Coding sequence (CDS)

ATGGAGCATTATTCCAAAGGGACAATTTGGTTAATACCAACGTTGTTGTTTGCCTGTCTGTATAGTGTTTCAAATGCATTGGATTGTGAGTTGAATTATCAAAATCCATACAGAGTTGCATATAGTATTGTTGTAAACAAATTTGGGAGAGGAAACTTCAGAACAATCCAAAGTGCTATTGATTCAATTCCTTTTGGAAGTACCAAATGGATTAGAGTTCAAATTTCTGCTGGCACATATTGGGAAAAGGTGACAATTCCCAAAGGAAAATCATGCATATTTATAGATGGGGCTGGCAGTAGGGTCACTGAAATTCAATGGAATGACCATCAAACCACAGCTACTAGTGCTACCTTCACTTCTTTTGCAGAAAACCTTGTAGTTCGAGGCATTGCATTCAGGAATACTTACAATGCACCAGGAAGTGTGAGGAGAAAAGAAGATATAGCACCAGCAGTTGCAGCTCTTATAATGGGAGACAAAGCAAGTTTCTATGAGTGTAGATTTATTGGACTGCAGGACACTCTGTGGGATGGACCTGGCCGTCATCATTTCGTCAATTGCTACATCGAAGGTGTAATCGATGTCATTTCTGGTTCTGGCCAATCCATCTACGAGAAATGTATGATAAATGTGCCAATCCATCAATATGCTCCAATATTGAGTTATGGATACATAACAGCACAAGGAAAAGAGAATCCAAATGAAGGCAGTGGCTTTGTTTTCAATGCATGCTCAGTTGTTGGAAATGGGAGAGTTTATCTTGGAAGGGCTTATAGGCCTTTCTCCACTGTCATTCTCCATAATTCTTTTCTAGCAGGTTTTATTGACCCTGCCGGCTGGAATCCTTGGGGACAAGTTGGCCATGAGACGAGTTTAACATATGCAGAGCTGAATTGTCGTGGACCCGGAGCAAACATTTCGAGGCGTGTGCCTTGGCTTAAGAGACTCCATAGAGCTGAGATTAGACATTTCACAGACATTTCTTTTATCGATCAAGAAGGTTGGACTTCAAGGCTTCCTCTACTT

Protein sequence

MEHYSKGTIWLIPTLLFACLYSVSNALDCELNYQNPYRVAYSIVVNKFGRGNFRTIQSAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWTSRLPLL

Homology

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BLAST of MS018972.1 vs. NCBI nr
Match: XP_022157021.1 (uncharacterized protein LOC111023848 [Momordica charantia])

HSP 1 Score: 702.6 bits (1812), Expect = 1.7e-198
Identity = 331/340 (97.35%), Postives = 334/340 (98.24%), Query Frame = 0


Query: 1   MEHYSKGTIWLIPTLLFACLYSVSNALDCELNYQNPYRVAYSIVVNKFGRGNFRTIQSAI 60
           MEHYSKGTIWLIPTLLFACLY+VSNAL CELNYQNPYRVAYSIVVNKFGRGNFRTIQSAI
Sbjct: 1   MEHYSKGTIWLIPTLLFACLYNVSNALACELNYQNPYRVAYSIVVNKFGRGNFRTIQSAI 60

Query: 61  DSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFT 120
           DSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFT
Sbjct: 61  DSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFT 120

Query: 121 SFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGP 180
           SFAENLVVRGIAFRNTYNAPGSVRR+EDIAPAVAALIMGDKASFYECRFIGLQDTLWDGP
Sbjct: 121 SFAENLVVRGIAFRNTYNAPGSVRRQEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGP 180

Query: 181 GRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGF 240
           GRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGF
Sbjct: 181 GRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGF 240

Query: 241 VFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNC 300
           VFNACSVVGNGRVYLGRAYRPFSTVI HNSFLAGFIDPAGWNPWGQVGHETSLTYAELNC
Sbjct: 241 VFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNC 300

Query: 301 RGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWTSRL 341
           RGPGANISRRVPWLKRLHRAEIRHFTDISFIDQE    +L
Sbjct: 301 RGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEALDCQL 340

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BLAST of MS018972.1 vs. NCBI nr
Match: XP_023524602.1 (LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.5 bits (1277), Expect = 1.8e-136
Identity = 231/334 (69.16%), Postives = 275/334 (82.34%), Query Frame = 0


Query: 9   IWLIPTLLFACLYSVSNALDCELNYQNPYRVAYSIVVNKFGRGNFRTIQSAIDSIPFGST 68
           I LI TLL     +VS ALDCELN  +P RVAY+IVV+K G GNFRTIQSAIDSIP  ++
Sbjct: 4   ILLITTLLLLLCLNVSKALDCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNS 63

Query: 69  KWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAENLVV 128
           +WIR+QIS+G Y EKV I + KSCIF++GAG   TEI+WNDH TTATSATFTS AENLVV
Sbjct: 64  RWIRIQISSGIYSEKVHISEEKSCIFLEGAGRSSTEIRWNDHATTATSATFTSIAENLVV 123

Query: 129 RGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNC 188
           +GI F+NTYN PGSV+R+EDIAPAVAALI GDK  F++C F+GLQDTLWDG GRH +  C
Sbjct: 124 KGITFKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTEC 183

Query: 189 YIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVV 248
           YIEGVIDVISG+GQSIYE C IN+P+ +YAP++ +GYITAQGKE+ ++ +GFVF  CSV+
Sbjct: 184 YIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVI 243

Query: 249 GNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANIS 308
           G+G VYLGRAYRPFSTVI ++SFL+  I+PAGW+PW QVGHE SLTY E  C GPGA+ S
Sbjct: 244 GSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTS 303

Query: 309 RRVPWLKRLHRAEIRHFTDISFIDQEGWTSRLPL 343
           +RVPWLK+L   EIR FT ISF+DQEGWTS+LPL
Sbjct: 304 KRVPWLKKLSIDEIRRFTSISFVDQEGWTSKLPL 337

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BLAST of MS018972.1 vs. NCBI nr
Match: KAG6606930.1 (putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496.1 bits (1276), Expect = 2.4e-136
Identity = 235/334 (70.36%), Postives = 275/334 (82.34%), Query Frame = 0


Query: 9   IWLIPTLLFACLYSVSNALDCELNYQNPYRVAYSIVVNKFGRGNFRTIQSAIDSIPFGST 68
           I LI TLL     +VS ALDCELN  +P RVAY+IVV+K G GNFRTIQSAIDSIP  ++
Sbjct: 4   ISLITTLLMLLCLNVSKALDCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNS 63

Query: 69  KWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAENLVV 128
           +WIR+QIS+G Y EKV I + KSCIF+DGAG   TEI+WNDH TTATSATFTS AENLVV
Sbjct: 64  RWIRIQISSGIYLEKVHISEEKSCIFLDGAGRSSTEIRWNDHATTATSATFTSIAENLVV 123

Query: 129 RGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNC 188
           +GI F+NTYNAPGSV+R+EDIAPAVAALI GDK  F++C F+GLQDTLWDG GRH +  C
Sbjct: 124 KGITFKNTYNAPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTEC 183

Query: 189 YIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVV 248
           YIEGVIDVISG+GQSIYE C INVP+  YAP++ +GYITAQGKE+ ++ +GFVF  CSV+
Sbjct: 184 YIEGVIDVISGAGQSIYENCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVI 243

Query: 249 GNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANIS 308
           G+G VYLGRAYRPFSTVI ++SFL+  IDPAGW+PW QVGHE SLT AE +C G GA+ S
Sbjct: 244 GSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLTCAETHCVGLGADTS 303

Query: 309 RRVPWLKRLHRAEIRHFTDISFIDQEGWTSRLPL 343
           +RV WLK+L   EIRHFT ISFIDQEGWTS+LPL
Sbjct: 304 KRVSWLKKLSIDEIRHFTSISFIDQEGWTSKLPL 337

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BLAST of MS018972.1 vs. NCBI nr
Match: KAE8650757.1 (hypothetical protein Csa_017626 [Cucumis sativus])

HSP 1 Score: 472.6 bits (1215), Expect = 2.8e-129
Identity = 223/337 (66.17%), Postives = 267/337 (79.23%), Query Frame = 0


Query: 1   MEHY--SKGTIWLIPTLLFACLYSVSNAL-DCELNYQNPYRVAYSIVVNKFGRGNFRTIQ 60
           MEHY   + ++++I TL+      VS AL DCELNY +P++VAY+IVV+K G+GNF TIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSA 120
           SAI+S+P  STKWIR+QIS G Y EKVTIP  KSCIF+DG+G +VTEI WNDH+TTA S 
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLW 180
           TFT+ A+NLVV+GI FRNTYNA GSV R+EDI PA+AALI GDK  F++C FIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEG 240
           DGPGRH F  CYIEGVIDVISG GQSIY++C+IN+P++ YAP+L+ G+ITAQGKENPNE 
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAE 300
           SGFVF  C V G+G V+LGRAYRPFSTVI H  FL   I+PAGWN W Q GHE+ LTY+E
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 LNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQE 335
             C GPGA+ S RVPW+ RL    IR FTDIS+ID +
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 337

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BLAST of MS018972.1 vs. NCBI nr
Match: XP_016900403.1 (PREDICTED: probable pectinesterase 55 [Cucumis melo])

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-126
Identity = 232/362 (64.09%), Postives = 269/362 (74.31%), Query Frame = 0


Query: 1   MEHYSKGTIWLIPTLLFA-CLYSVSNALD-CELNYQNPYRVAYSIVVNKFGRGNFRTIQS 60
           MEHYSK    LI TLL   C  +VS ALD CELNY +P++VAY+IVV+KFGRGNF TIQS
Sbjct: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60

Query: 61  AIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSAT 120
           AIDS+P  S +WIR+QIS G Y               DG+G RVTEIQWNDH TTATS T
Sbjct: 61  AIDSVPSQSNRWIRIQISPGIY-------------TYDGSGFRVTEIQWNDHATTATSPT 120

Query: 121 FTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWD 180
           FTS+A+NLVV+GI FRNTYNA GSV R+EDI PA+AALI GDKA F++C FIGLQDTLWD
Sbjct: 121 FTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWD 180

Query: 181 GPGRHHFVNCYIEGVIDVISGSGQSI----------------YEKCMINVPIHQYAPILS 240
           GPGRH F  CYIEGVIDVISG G  I                 ++C+IN+PI+ YAP+L+
Sbjct: 181 GPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLN 240

Query: 241 YGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWN 300
            G+ITAQGKENP+E SGFVF  C V G+G+V+LGRAYRPFSTVI H  FL   IDPAGWN
Sbjct: 241 EGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWN 300

Query: 301 PWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEG-WTSRLP 344
            W QVGHE++LTY+E  C GPGA+ S RVPWLKRL   +IR FTDIS+ID +G WTSRLP
Sbjct: 301 SWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLP 349

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BLAST of MS018972.1 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.1e-87
Identity = 150/303 (49.50%), Postives = 200/303 (66.01%), Query Frame = 0


Query: 41  YSIVVNKFGRGNFRTIQSAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGS 100
           + ++V++ G GNF TIQ AIDS+P  +T W  + + AG Y EK+TIP+ K  I I GAG 
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGK 86

Query: 101 RVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVR-RKEDIAPAVAALIMG 160
           R T ++W+DH + A S TF + A+N VV+ I F N+YN P + +  K    PAVAA I G
Sbjct: 87  RSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGG 146

Query: 161 DKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAP 220
           DK++FY   F G+QDTLWD  GRH+F  C I+G +D I GSGQSIY+ C+I V   Q  P
Sbjct: 147 DKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGP 206

Query: 221 ILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPA 280
            ++ GYITAQG+ N N+ +GFVF  C V G G+ YLGRA+RP+S VI +NS L   +DP 
Sbjct: 207 GVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPL 266

Query: 281 GWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWTSR 340
           GW  W   G+E  LTYAE  C G G+N SRR  W+K+L  + ++H  D+SFI++ GW   
Sbjct: 267 GWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVED 326

Query: 341 LPL 343
           LP+
Sbjct: 327 LPI 328

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BLAST of MS018972.1 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.4e-84
Identity = 151/338 (44.67%), Postives = 210/338 (62.13%), Query Frame = 0


Query: 7   GTIWLIPTLLFACLYSVSNALDCELNYQNPYRV-AYSIVVNKFGRGNFRTIQSAIDSIPF 66
           GT  +   L+  C + + + ++ +     P+ V    + V++ G GNF TIQ AIDS+P 
Sbjct: 2   GTHRIFIGLIALCCFCLPHLIEAK-----PFGVYQQQVFVDQSGHGNFTTIQKAIDSVPI 61

Query: 67  GSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAEN 126
            +  W  + + AG Y EK+ IP  K  I + GAG R+T ++W+DH + A S TF++ A+N
Sbjct: 62  NNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADN 121

Query: 127 LVVRGIAFRNTYNAPGSVR-RKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHH 186
            VV+ I F N+YN P   +  K    PAVAALI GDK++FY   F G+QDTLWD  GRH+
Sbjct: 122 TVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHY 181

Query: 187 FVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNA 246
           F  C I+G +D I G+GQSIY+ C+I V   Q  P L+ GYITAQG+ NP + +GF+F  
Sbjct: 182 FHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGLA-GYITAQGRTNPYDANGFIFIN 241

Query: 247 CSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPG 306
           C V G G  +LGR +R +S VI +NS L   + P GW+ W  VGHE  L +AE  C G G
Sbjct: 242 CLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSG 301

Query: 307 ANISRRVPWLKRLHRAEIRHFTDISFIDQEGWTSRLPL 343
           ANI RRV W+K+L  + I++  D+SFI++ GW   LP+
Sbjct: 302 ANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLPI 333

[Close]

BLAST of MS018972.1 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 303.9 bits (777), Expect = 2.3e-81
Identity = 145/305 (47.54%), Postives = 201/305 (65.90%), Query Frame = 0


Query: 38  RVAYSIVVNKFGRGNFRTIQSAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDG 97
           ++AY+I V+  G GNF T+QSAIDSI   +  WIRV    G Y EKVTIPK K  I++ G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 98  AGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALI 157
            G   T I+++DHQ T  SATFT+FA+++V+ GI F+NTYN   +   K +I PAVAA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 158 MGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQY 217
           +GD+    +  F+GLQDTL+DG GRH++  C I G ID I G GQS++++C +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 218 APILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFID 277
           AP   YG ITA  + +P++  GFVF+ C+V G G+  LGRA+   + VI   S L+  + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 278 PAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWT 337
           P GW+ W   G+E  LT+ E  C G GA+ S+RVPWLK+L  +E+  F  +SFIDQ+GW 
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDGWI 329

Query: 338 SRLPL 343
           SR P+
Sbjct: 330 SRFPI 332

[Close]

BLAST of MS018972.1 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-80
Identity = 140/305 (45.90%), Postives = 195/305 (63.93%), Query Frame = 0


Query: 38  RVAYSIVVNKFGRGNFRTIQSAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDG 97
           ++A +I+VN      F+T+QSAIDSIP  +  WIR+ IS G Y EKVTIP+GK  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 98  AGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALI 157
            G   T I + DHQ T TSATFTS+  N+++ GI F+N YN   S        PAVAA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 158 MGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQY 217
           +GDK +  +  F G QDTL+D  GRH++  C I G ID I G  QSI+E C + + +  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 218 APILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFID 277
            P   YG ITAQG+++P +  GFVF  C+V+G+G+  LGRA++ +S VI + S  +  I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 278 PAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWT 337
           P GW+ W   G E  +T+ E  C G GA+ S+RVPWL +    ++  FT+++FID+EGW 
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWL 335

Query: 338 SRLPL 343
           SRLP+
Sbjct: 336 SRLPI 337

[Close]

BLAST of MS018972.1 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 263.8 bits (673), Expect = 2.6e-69
Identity = 126/250 (50.40%), Postives = 164/250 (65.60%), Query Frame = 0


Query: 97  GAGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRRKE----DIAPA 156
           G G RVT I +N H  T  S+TFTS+  ++VVR ++  NTYN   S+ +      DI PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 157 VAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINV 216
           VA  + GDK++FY C F+GLQDT+WD  GRHHF NCYIEG ID I GSGQS+YE C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 217 PIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFL 276
                A  +S+GYITAQG+ + ++ SGFVF   SV G+  VYLGRAY PFS VI   + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 277 AGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIR-HFTDISFI 336
           +  + P GW  W   G+E S TYAE+ C+G G+++SRRVPW+ +LH    +  F+  +FI
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 337 DQEGWTSRLP 342
           DQ+ W S +P
Sbjct: 282 DQDQWISNIP 291

[Close]

BLAST of MS018972.1 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 8.2e-199
Identity = 331/340 (97.35%), Postives = 334/340 (98.24%), Query Frame = 0


Query: 1   MEHYSKGTIWLIPTLLFACLYSVSNALDCELNYQNPYRVAYSIVVNKFGRGNFRTIQSAI 60
           MEHYSKGTIWLIPTLLFACLY+VSNAL CELNYQNPYRVAYSIVVNKFGRGNFRTIQSAI
Sbjct: 1   MEHYSKGTIWLIPTLLFACLYNVSNALACELNYQNPYRVAYSIVVNKFGRGNFRTIQSAI 60

Query: 61  DSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFT 120
           DSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFT
Sbjct: 61  DSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFT 120

Query: 121 SFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGP 180
           SFAENLVVRGIAFRNTYNAPGSVRR+EDIAPAVAALIMGDKASFYECRFIGLQDTLWDGP
Sbjct: 121 SFAENLVVRGIAFRNTYNAPGSVRRQEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGP 180

Query: 181 GRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGF 240
           GRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGF
Sbjct: 181 GRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGF 240

Query: 241 VFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNC 300
           VFNACSVVGNGRVYLGRAYRPFSTVI HNSFLAGFIDPAGWNPWGQVGHETSLTYAELNC
Sbjct: 241 VFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNC 300

Query: 301 RGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWTSRL 341
           RGPGANISRRVPWLKRLHRAEIRHFTDISFIDQE    +L
Sbjct: 301 RGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEALDCQL 340

[Close]

BLAST of MS018972.1 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 5.4e-134
Identity = 231/347 (66.57%), Postives = 276/347 (79.54%), Query Frame = 0


Query: 1   MEHY--SKGTIWLIPTLLFACLYSVSNAL-DCELNYQNPYRVAYSIVVNKFGRGNFRTIQ 60
           MEHY   + ++++I TL+      VS AL DCELNY +P++VAY+IVV+K G+GNF TIQ
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSA 120
           SAI+S+P  STKWIR+QIS G Y EKVTIP  KSCIF+DG+G +VTEI WNDH+TTA S 
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLW 180
           TFT+ A+NLVV+GI FRNTYNA GSV R+EDI PA+AALI GDK  F++C FIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEG 240
           DGPGRH F  CYIEGVIDVISG GQSIY++C+IN+P++ YAP+L+ G+ITAQGKENPNE 
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAE 300
           SGFVF  C V G+G V+LGRAYRPFSTVI H  FL   I+PAGWN W Q GHE+ LTY+E
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 LNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEG-WTSRLPLL 344
             C GPGA+ S RVPW+ RL    IR FTDIS+ID +G WTSR+PLL
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLL 347

[Close]

BLAST of MS018972.1 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 6.4e-127
Identity = 232/362 (64.09%), Postives = 269/362 (74.31%), Query Frame = 0


Query: 1   MEHYSKGTIWLIPTLLFA-CLYSVSNALD-CELNYQNPYRVAYSIVVNKFGRGNFRTIQS 60
           MEHYSK    LI TLL   C  +VS ALD CELNY +P++VAY+IVV+KFGRGNF TIQS
Sbjct: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60

Query: 61  AIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSAT 120
           AIDS+P  S +WIR+QIS G Y               DG+G RVTEIQWNDH TTATS T
Sbjct: 61  AIDSVPSQSNRWIRIQISPGIY-------------TYDGSGFRVTEIQWNDHATTATSPT 120

Query: 121 FTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWD 180
           FTS+A+NLVV+GI FRNTYNA GSV R+EDI PA+AALI GDKA F++C FIGLQDTLWD
Sbjct: 121 FTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWD 180

Query: 181 GPGRHHFVNCYIEGVIDVISGSGQSI----------------YEKCMINVPIHQYAPILS 240
           GPGRH F  CYIEGVIDVISG G  I                 ++C+IN+PI+ YAP+L+
Sbjct: 181 GPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLN 240

Query: 241 YGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWN 300
            G+ITAQGKENP+E SGFVF  C V G+G+V+LGRAYRPFSTVI H  FL   IDPAGWN
Sbjct: 241 EGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWN 300

Query: 301 PWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEG-WTSRLP 344
            W QVGHE++LTY+E  C GPGA+ S RVPWLKRL   +IR FTDIS+ID +G WTSRLP
Sbjct: 301 SWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLP 349

[Close]

BLAST of MS018972.1 vs. ExPASy TrEMBL
Match: A0A061DSC1 (Pectinesterase OS=Theobroma cacao OX=3641 GN=TCM_004838 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 2.0e-104
Identity = 176/332 (53.01%), Postives = 239/332 (71.99%), Query Frame = 0


Query: 12  IPTLLFACL--YSVSNALDCELNYQNPYRVAYSIVVNKFGRGNFRTIQSAIDSIPFGSTK 71
           I  L+F  L  +S+S AL+C+LN  N ++VA++I V++ G+G+F+TIQ+AIDSIP  + K
Sbjct: 23  ILALIFIVLLYFSISKALECQLNNANKFKVAHTIRVDQSGKGDFKTIQNAIDSIPSNNDK 82

Query: 72  WIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAENLVVR 131
           WIR+ IS G + EKVTIP  K CIF++GAGS++T I+W DH  T  SATFTS  +N+V +
Sbjct: 83  WIRILISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTSSPDNIVAK 142

Query: 132 GIAFRNTYNAPGSVRRKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNCY 191
           GI+F+N YN P     K+ + PA+A  I GDK++FY C F GLQDTLWD  GRH+F NCY
Sbjct: 143 GISFKNIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYNCY 202

Query: 192 IEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVG 251
           IEG +D I GSGQSIYEKC IN+   +YAP    GYITAQG+ + ++ SGFVF  C   G
Sbjct: 203 IEGAVDFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFVFKYCVFTG 262

Query: 252 NGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANISR 311
            G+ YLGRAY  +S VI++ S L+  I  +GW+ W  V HE +L Y E +C+GPGAN S+
Sbjct: 263 YGKTYLGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCKGPGANTSK 322

Query: 312 RVPWLKRLHRAEIRHFTDISFIDQEGWTSRLP 342
           RVPWLK+L  ++++ F ++S+ID+EGW ++LP
Sbjct: 323 RVPWLKKLGASQLKKFLNLSYIDKEGWIAKLP 354

[Close]

BLAST of MS018972.1 vs. ExPASy TrEMBL
Match: A0A5D3DA76 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 3.4e-104
Identity = 183/259 (70.66%), Postives = 211/259 (81.47%), Query Frame = 0


Query: 86  IPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRR 145
           IP  KSCIF+DG+G RVTEIQWNDH TTATS TFTS+A+NLV       NTYNA GSV R
Sbjct: 2   IPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLV-------NTYNALGSVTR 61

Query: 146 KEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIY 205
           +EDI PA+AALI GDKA F++C FIGLQDTLWDGPGRH F  CYIEGVIDVISG GQSIY
Sbjct: 62  REDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIY 121

Query: 206 EKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTV 265
           ++C+IN+PI+ YAP+L+ G+ITAQGKENP+E SGFVF  C V G+G+V+LGRAYRPFSTV
Sbjct: 122 KECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTV 181

Query: 266 ILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHF 325
           I H  FL   IDPAGWN W QVGHE++LTY+E  C GPGA+ S RVPWLKRL   +IR F
Sbjct: 182 IFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRF 241

Query: 326 TDISFIDQEG-WTSRLPLL 344
           TDIS+ID +G WTSRLP+L
Sbjct: 242 TDISYIDPQGYWTSRLPVL 253

[Close]

BLAST of MS018972.1 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.5e-88
Identity = 150/303 (49.50%), Postives = 200/303 (66.01%), Query Frame = 0


Query: 41  YSIVVNKFGRGNFRTIQSAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGS 100
           + ++V++ G GNF TIQ AIDS+P  +T W  + + AG Y EK+TIP+ K  I I GAG 
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGK 86

Query: 101 RVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVR-RKEDIAPAVAALIMG 160
           R T ++W+DH + A S TF + A+N VV+ I F N+YN P + +  K    PAVAA I G
Sbjct: 87  RSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGG 146

Query: 161 DKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAP 220
           DK++FY   F G+QDTLWD  GRH+F  C I+G +D I GSGQSIY+ C+I V   Q  P
Sbjct: 147 DKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGP 206

Query: 221 ILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPA 280
            ++ GYITAQG+ N N+ +GFVF  C V G G+ YLGRA+RP+S VI +NS L   +DP 
Sbjct: 207 GVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPL 266

Query: 281 GWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWTSR 340
           GW  W   G+E  LTYAE  C G G+N SRR  W+K+L  + ++H  D+SFI++ GW   
Sbjct: 267 GWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVED 326

Query: 341 LPL 343
           LP+
Sbjct: 327 LPI 328

[Close]

BLAST of MS018972.1 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 6.0e-85
Identity = 151/338 (44.67%), Postives = 210/338 (62.13%), Query Frame = 0


Query: 7   GTIWLIPTLLFACLYSVSNALDCELNYQNPYRV-AYSIVVNKFGRGNFRTIQSAIDSIPF 66
           GT  +   L+  C + + + ++ +     P+ V    + V++ G GNF TIQ AIDS+P 
Sbjct: 2   GTHRIFIGLIALCCFCLPHLIEAK-----PFGVYQQQVFVDQSGHGNFTTIQKAIDSVPI 61

Query: 67  GSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAEN 126
            +  W  + + AG Y EK+ IP  K  I + GAG R+T ++W+DH + A S TF++ A+N
Sbjct: 62  NNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADN 121

Query: 127 LVVRGIAFRNTYNAPGSVR-RKEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHH 186
            VV+ I F N+YN P   +  K    PAVAALI GDK++FY   F G+QDTLWD  GRH+
Sbjct: 122 TVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHY 181

Query: 187 FVNCYIEGVIDVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNA 246
           F  C I+G +D I G+GQSIY+ C+I V   Q  P L+ GYITAQG+ NP + +GF+F  
Sbjct: 182 FHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGLA-GYITAQGRTNPYDANGFIFIN 241

Query: 247 CSVVGNGRVYLGRAYRPFSTVILHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPG 306
           C V G G  +LGR +R +S VI +NS L   + P GW+ W  VGHE  L +AE  C G G
Sbjct: 242 CLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSG 301

Query: 307 ANISRRVPWLKRLHRAEIRHFTDISFIDQEGWTSRLPL 343
           ANI RRV W+K+L  + I++  D+SFI++ GW   LP+
Sbjct: 302 ANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLPI 333

[Close]

BLAST of MS018972.1 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.6e-82
Identity = 145/305 (47.54%), Postives = 201/305 (65.90%), Query Frame = 0


Query: 38  RVAYSIVVNKFGRGNFRTIQSAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDG 97
           ++AY+I V+  G GNF T+QSAIDSI   +  WIRV    G Y EKVTIPK K  I++ G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 98  AGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALI 157
            G   T I+++DHQ T  SATFT+FA+++V+ GI F+NTYN   +   K +I PAVAA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 158 MGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQY 217
           +GD+    +  F+GLQDTL+DG GRH++  C I G ID I G GQS++++C +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 218 APILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFID 277
           AP   YG ITA  + +P++  GFVF+ C+V G G+  LGRA+   + VI   S L+  + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 278 PAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWT 337
           P GW+ W   G+E  LT+ E  C G GA+ S+RVPWLK+L  +E+  F  +SFIDQ+GW 
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDGWI 329

Query: 338 SRLPL 343
           SR P+
Sbjct: 330 SRFPI 332

[Close]

BLAST of MS018972.1 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-81
Identity = 140/305 (45.90%), Postives = 195/305 (63.93%), Query Frame = 0


Query: 38  RVAYSIVVNKFGRGNFRTIQSAIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDG 97
           ++A +I+VN      F+T+QSAIDSIP  +  WIR+ IS G Y EKVTIP+GK  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 98  AGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRRKEDIAPAVAALI 157
            G   T I + DHQ T TSATFTS+  N+++ GI F+N YN   S        PAVAA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 158 MGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINVPIHQY 217
           +GDK +  +  F G QDTL+D  GRH++  C I G ID I G  QSI+E C + + +  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 218 APILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFLAGFID 277
            P   YG ITAQG+++P +  GFVF  C+V+G+G+  LGRA++ +S VI + S  +  I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 278 PAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQEGWT 337
           P GW+ W   G E  +T+ E  C G GA+ S+RVPWL +    ++  FT+++FID+EGW 
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWL 335

Query: 338 SRLPL 343
           SRLP+
Sbjct: 336 SRLPI 337

[Close]

BLAST of MS018972.1 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.9e-70
Identity = 126/250 (50.40%), Postives = 164/250 (65.60%), Query Frame = 0


Query: 97  GAGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPGSVRRKE----DIAPA 156
           G G RVT I +N H  T  S+TFTS+  ++VVR ++  NTYN   S+ +      DI PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 157 VAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSGQSIYEKCMINV 216
           VA  + GDK++FY C F+GLQDT+WD  GRHHF NCYIEG ID I GSGQS+YE C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 217 PIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVILHNSFL 276
                A  +S+GYITAQG+ + ++ SGFVF   SV G+  VYLGRAY PFS VI   + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 277 AGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIR-HFTDISFI 336
           +  + P GW  W   G+E S TYAE+ C+G G+++SRRVPW+ +LH    +  F+  +FI
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 337 DQEGWTSRLP 342
           DQ+ W S +P
Sbjct: 282 DQDQWISNIP 291

The following BLAST results are available for this feature:

BLAST of MS018972.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157021.1	1.7e-198	97.35	uncharacterized protein LOC111023848 [Momordica charantia]	[more]
XP_023524602.1	1.8e-136	69.16	LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo]	[more]
KAG6606930.1	2.4e-136	70.36	putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAE8650757.1	2.8e-129	66.17	hypothetical protein Csa_017626 [Cucumis sativus]	[more]
XP_016900403.1	1.3e-126	64.09	PREDICTED: probable pectinesterase 55 [Cucumis melo]	[more]

BLAST of MS018972.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E9D3	2.1e-87	49.50	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
Q4PSN0	8.4e-84	44.67	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
Q4PSQ5	2.3e-81	47.54	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
O64479	1.5e-80	45.90	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
O04953	2.6e-69	50.40	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]

BLAST of MS018972.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWQ8	8.2e-199	97.35	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1	[more]
A0A0A0LB12	5.4e-134	66.57	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1	[more]
A0A1S4DWQ1	6.4e-127	64.09	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1	[more]
A0A061DSC1	2.0e-104	53.01	Pectinesterase OS=Theobroma cacao OX=3641 GN=TCM_004838 PE=4 SV=1	[more]
A0A5D3DA76	3.4e-104	70.66	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550...	[more]

BLAST of MS018972.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G18990.1	1.5e-88	49.50	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	6.0e-85	44.67	Pectin lyase-like superfamily protein	[more]
AT2G47280.1	1.6e-82	47.54	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	1.1e-81	45.90	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	1.9e-70	50.40	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 43..333 e-value: 1.6E-57 score: 194.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 37..341 e-value: 6.7E-90 score: 303.7
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 16..342
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 16..342
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..19 score: 5.0
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 39..338

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS018972	MS018972	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS018972.1-cds	MS018972.1-cds-scaffold20:381230..381471	CDS
MS018972.1-cds	MS018972.1-cds-scaffold20:381749..381908	CDS
MS018972.1-cds	MS018972.1-cds-scaffold20:382712..382927	CDS
MS018972.1-cds	MS018972.1-cds-scaffold20:383562..383812	CDS
MS018972.1-cds	MS018972.1-cds-scaffold20:383907..384066	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS018972.1	MS018972.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity