MS018834.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS018834.1 vs. NCBI nr
Match: XP_022137219.1 (probable cyclic nucleotide-gated ion channel 20, chloroplastic [Momordica charantia] >XP_022137220.1 probable cyclic nucleotide-gated ion channel 20, chloroplastic [Momordica charantia] >XP_022137221.1 probable cyclic nucleotide-gated ion channel 20, chloroplastic [Momordica charantia])

HSP 1 Score: 1553.5 bits (4021), Expect = 0.0e+00
Identity = 772/772 (100.00%), Postives = 772/772 (100.00%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT
Sbjct: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH
Sbjct: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW
Sbjct: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI
Sbjct: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG
Sbjct: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
           KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS
Sbjct: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID
Sbjct: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420

Query: 421 CGHGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFW 480
           CGHGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFW
Sbjct: 421 CGHGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFW 480

Query: 481 GFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRR 540
           GFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRR 540

Query: 541 RDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600
           RDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 601 KVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVDGIVVP 660
           KVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVDGIVVP
Sbjct: 601 KVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVDGIVVP 660

Query: 661 LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEV 720
           LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEV
Sbjct: 661 LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEV 720

Query: 721 TSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           TSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN
Sbjct: 721 TSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 772

BLAST of MS018834.1 vs. NCBI nr
Match: XP_038893877.1 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 674/775 (86.97%), Postives = 715/775 (92.26%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPPM DEQ  SYFPS+ASL RSSSLSIP TSSG+YG+EANLV HT
Sbjct: 1   MAAFEKDDIPMLSNTDPPMFDEQADSYFPSYASLGRSSSLSIPATSSGMYGSEANLVAHT 60

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPL+  R SSFMVSGS YTG KPE+LSQS PGV ESKTAEPLA+KFPSFK  DESDWSIH
Sbjct: 61  GPLQIGR-SSFMVSGSKYTGGKPEKLSQSKPGVTESKTAEPLAEKFPSFKTTDESDWSIH 120

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLI+SGQLG+C+DPFCITCPTYN KAS+ KS RMSGIFD KF +D +G GKGW
Sbjct: 121 NYAGRNEHLIRSGQLGLCNDPFCITCPTYNFKASQQKSSRMSGIFDAKFHNDLYGGGKGW 180

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKF++FW FLLPYIPGVMNPHAKV+QQWNKFFVI+CLVAIFLDPLFFFLL+V+E  KCI
Sbjct: 181 AGKFQSFWCFLLPYIPGVMNPHAKVIQQWNKFFVIACLVAIFLDPLFFFLLSVQEKNKCI 240

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VI+WPLTTTLV FRS+TDFIY L+MLLQFRLAYV+PE+ VVGAGELVD PK+IA++Y+KG
Sbjct: 241 VIDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAMNYLKG 300

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+DLLVVLPLPQII+FLVLPKSLGSSGANYAKNLLR AVTVQYIPRLYRFLPLLAGQS
Sbjct: 301 NFFIDLLVVLPLPQIIIFLVLPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQS 360

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFR  C  T +  CL+YID
Sbjct: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRKVCVDTKNLSCLEYID 420

Query: 421 CGHGDEAWS-PDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDEA S   +  R  W  N NA ACFAKE FDYGIYLQAVNLTT+NSIITRYTYSLF
Sbjct: 421 CGHGDEAGSLIPNSQRDSWINNENASACFAKEGFDYGIYLQAVNLTTENSIITRYTYSLF 480

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTM IIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR
Sbjct: 481 WGFQQISTLAGNQVPSYYVYEVLFTMAIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLP NLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV
Sbjct: 541 RRDVEQWMEHRRLPHNLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFS+GDIIEKMVFIVRGKMESRVD  GI
Sbjct: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDIIEKMVFIVRGKMESRVDGNGI 660

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+N+DMKR QVPAPRLVSNRTV+CL+NVEAFSLRAADL
Sbjct: 661 VVPLSEGDVCGEELLTWCLEHSSINKDMKRHQVPAPRLVSNRTVKCLSNVEAFSLRAADL 720

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA  RIQVAWRYRK+RLSR QTS+SN
Sbjct: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAAMRIQVAWRYRKRRLSRVQTSQSN 774

BLAST of MS018834.1 vs. NCBI nr
Match: XP_011649574.1 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 [Cucumis sativus] >XP_031742793.1 probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 [Cucumis sativus] >KGN63956.2 hypothetical protein Csa_013104 [Cucumis sativus])

HSP 1 Score: 1342.8 bits (3474), Expect = 0.0e+00
Identity = 673/775 (86.84%), Postives = 715/775 (92.26%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPPM DEQV SYFPS+ASL RSSSLSIP  SSG+YG+EANLVGHT
Sbjct: 1   MAAFEKDDIPMLSNTDPPMFDEQVDSYFPSYASLGRSSSLSIPENSSGMYGSEANLVGHT 60

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLR +R SSFMVSGS  TG KP++LSQS PGV ESKTAEPLA+KFPSFK  DESDWSIH
Sbjct: 61  GPLRIER-SSFMVSGSKKTGSKPKKLSQSKPGVTESKTAEPLAEKFPSFKIVDESDWSIH 120

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLG+C+DPFCITCPTYN KAS+ KS RMSGIFD KF +D +G GKGW
Sbjct: 121 NYAGRNEHLIKSGQLGLCNDPFCITCPTYNSKASQQKSSRMSGIFDAKFHNDLYGGGKGW 180

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           A KF++FW FLL YIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLL+V+E+ KCI
Sbjct: 181 ARKFQSFWCFLLSYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLSVQENNKCI 240

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VI+WPLTTTLV FRS+TDFIY L+MLLQFRLAYV+PES VVGAGELVD PK+IA++Y+KG
Sbjct: 241 VIDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPESRVVGAGELVDHPKKIAMNYLKG 300

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+DLLVVLPLPQII+FL+LPKSLGSSGANYAKNLLR AVTVQYIPRLYRFLPLLAGQS
Sbjct: 301 NFFIDLLVVLPLPQIIIFLILPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQS 360

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQ V RCFR+AC  TG+R CL+YID
Sbjct: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQRVTRCFREACINTGNRSCLEYID 420

Query: 421 CGHGDEAWSPDHEPRR-KWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDE+ S   +P R  W  N NA ACF  E FDYGIYLQAV LTTKNSIITRYTYSLF
Sbjct: 421 CGHGDESRSQILDPHRDSWTHNVNATACFGSEGFDYGIYLQAVKLTTKNSIITRYTYSLF 480

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR
Sbjct: 481 WGFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLPE LRRQVRQAERYNWASTRGVNEERIFENLPEDLQ+NIRRHLF+FV
Sbjct: 541 RRDVEQWMEHRRLPEGLRRQVRQAERYNWASTRGVNEERIFENLPEDLQKNIRRHLFKFV 600

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFS+GDIIEKMVFIVRGKMESRVD  GI
Sbjct: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDIIEKMVFIVRGKMESRVDGNGI 660

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+N+DMKR QVPAPRLVSNRTVRCL+NVEAFSLRA DL
Sbjct: 661 VVPLSEGDVCGEELLTWCLEHSSLNKDMKRQQVPAPRLVSNRTVRCLSNVEAFSLRAVDL 720

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA  RIQVAWRYRK+RLSR QTS+SN
Sbjct: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAAMRIQVAWRYRKRRLSRVQTSQSN 774

BLAST of MS018834.1 vs. NCBI nr
Match: XP_008467252.1 (PREDICTED: probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 667/775 (86.06%), Postives = 713/775 (92.00%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPP+ DEQ+ SYFPS+ASL RSSSLSIP  SSG+Y +EANLVGHT
Sbjct: 25  MAAFEKDDIPMLSNTDPPIFDEQMDSYFPSYASLGRSSSLSIPANSSGMYSSEANLVGHT 84

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLR +R SSF VSGS  T  KP++LSQS PGV ESKTAEPLA+KFPSFK  D+ DWSIH
Sbjct: 85  GPLRIER-SSFAVSGSKNTDSKPKKLSQSKPGVTESKTAEPLAEKFPSFKIVDDPDWSIH 144

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLG+C+DPFCITCPTYN K S+ KS R+SGIFD KF SD +G GKGW
Sbjct: 145 NYAGRNEHLIKSGQLGLCNDPFCITCPTYNSKVSQQKSSRISGIFDAKFHSDLYGGGKGW 204

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKF++FW FLL YIPGVMNPHAKVVQQWNKFFVISCL+AIFLDPLFFFLL+V+E  KCI
Sbjct: 205 AGKFQSFWCFLLSYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFLLSVQEKNKCI 264

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VI+WPLTTTLV FRS+TDFIY L+MLLQFRLAYV+PES VVGAGELVD PK+IA++Y+KG
Sbjct: 265 VIDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPESRVVGAGELVDHPKKIAMNYLKG 324

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+DLLVVLPLPQII+FLVLPKSLGSSGANYAKNLLR AVTVQYIPRLYRFLPLLAGQS
Sbjct: 325 NFFIDLLVVLPLPQIIIFLVLPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQS 384

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQ V RCFRDACNKTG+R CL+YID
Sbjct: 385 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQRVTRCFRDACNKTGNRSCLEYID 444

Query: 421 CGHGDEAWSPDHEPRR-KWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDE+ S   +P R  W  N NA ACF+ E F YGIYLQAVNLTT+ SI+TRYTYSLF
Sbjct: 445 CGHGDESRSQFLDPHRDSWKHNENATACFSNEGFGYGIYLQAVNLTTEKSILTRYTYSLF 504

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR
Sbjct: 505 WGFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 564

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQ+NIRRHLF+FV
Sbjct: 565 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQKNIRRHLFKFV 624

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFS+GD IEKMVFIVRGKMESRVD  GI
Sbjct: 625 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDTIEKMVFIVRGKMESRVDGNGI 684

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+N+DMKR QVPAPRLVSNRTVRCL+NVEAFSLRAADL
Sbjct: 685 VVPLSEGDVCGEELLTWCLEHSSINKDMKRQQVPAPRLVSNRTVRCLSNVEAFSLRAADL 744

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA  RIQVAWRYRK+RLSR QTS+SN
Sbjct: 745 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAAMRIQVAWRYRKRRLSRVQTSQSN 798

BLAST of MS018834.1 vs. NCBI nr
Match: KAA0036044.1 (putative cyclic nucleotide-gated ion channel 20 [Cucumis melo var. makuwa])

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 667/775 (86.06%), Postives = 713/775 (92.00%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPP+ DEQ+ SYFPS+ASL RSSSLSIP  SSG+Y +EANLVGHT
Sbjct: 91  MAAFEKDDIPMLSNTDPPIFDEQMDSYFPSYASLGRSSSLSIPANSSGMYSSEANLVGHT 150

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLR +R SSF VSGS  T  KP++LSQS PGV ESKTAEPLA+KFPSFK  D+ DWSIH
Sbjct: 151 GPLRIER-SSFAVSGSKNTDSKPKKLSQSKPGVTESKTAEPLAEKFPSFKIVDDPDWSIH 210

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLG+C+DPFCITCPTYN K S+ KS R+SGIFD KF SD +G GKGW
Sbjct: 211 NYAGRNEHLIKSGQLGLCNDPFCITCPTYNSKVSQQKSSRISGIFDAKFHSDLYGGGKGW 270

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKF++FW FLL YIPGVMNPHAKVVQQWNKFFVISCL+AIFLDPLFFFLL+V+E  KCI
Sbjct: 271 AGKFQSFWCFLLSYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFLLSVQEKNKCI 330

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VI+WPLTTTLV FRS+TDFIY L+MLLQFRLAYV+PES VVGAGELVD PK+IA++Y+KG
Sbjct: 331 VIDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPESRVVGAGELVDHPKKIAMNYLKG 390

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+DLLVVLPLPQII+FLVLPKSLGSSGANYAKNLLR AVTVQYIPRLYRFLPLLAGQS
Sbjct: 391 NFFIDLLVVLPLPQIIIFLVLPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQS 450

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQ V RCFRDACNKTG+R CL+YID
Sbjct: 451 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQRVTRCFRDACNKTGNRSCLEYID 510

Query: 421 CGHGDEAWSPDHEPRR-KWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDE+ S   +P R  W  N NA ACF+ E F YGIYLQAVNLTT+ SI+TRYTYSLF
Sbjct: 511 CGHGDESRSQFLDPHRDSWKHNENATACFSNEGFGYGIYLQAVNLTTEKSILTRYTYSLF 570

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR
Sbjct: 571 WGFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 630

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQ+NIRRHLF+FV
Sbjct: 631 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQKNIRRHLFKFV 690

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFS+GD IEKMVFIVRGKMESRVD  GI
Sbjct: 691 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDTIEKMVFIVRGKMESRVDGNGI 750

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+N+DMKR QVPAPRLVSNRTVRCL+NVEAFSLRAADL
Sbjct: 751 VVPLSEGDVCGEELLTWCLEHSSINKDMKRQQVPAPRLVSNRTVRCLSNVEAFSLRAADL 810

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA  RIQVAWRYRK+RLSR QTS+SN
Sbjct: 811 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAAMRIQVAWRYRKRRLSRVQTSQSN 864

BLAST of MS018834.1 vs. ExPASy Swiss-Prot
Match: Q9LD37 (Probable cyclic nucleotide-gated ion channel 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CNGC20 PE=2 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 7.0e-264
Identity = 468/771 (60.70%), Postives = 592/771 (76.78%), Query Frame = 0

Query: 5   EKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHTGPLR 64
           E DDIPML     P+ D    +   +F S +RS SLS PT+S   +     ++G+TGPLR
Sbjct: 6   ENDDIPML-----PISDPSSRTRARAFTSRSRSVSLSNPTSSIEGFDTSTVVLGYTGPLR 65

Query: 65  SDRKSSFM-VSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIHNYA 124
           + R+   + +SG   + RK E L   +P   +S       +++PSF A +  + S   + 
Sbjct: 66  TQRRPPLVQMSGPLTSTRKHEPLFLPHPS-SDSVGVSSQPERYPSFAALEHKNSSEDEFV 125

Query: 125 GRNEHLIKSGQLGMCSDPFCITCPT-YNLKASKHKSLRMSGIFDHKFQSDFFGDGKGWAG 184
            ++ +L++SGQLGMC+DP+C TCP+ YN KA++  + R+S +FD  F +  + D KGWA 
Sbjct: 126 LKHANLLRSGQLGMCNDPYCTTCPSYYNRKAAQIPTSRVSALFDSTFHNALYDDAKGWA- 185

Query: 185 KFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVI 244
             R F S +  Y+PG+MNPHAK VQ W KFF +SCL+AIF+DPLFFFL+ V+E  KCI+I
Sbjct: 186 --RRFASSVNRYLPGIMNPHAKEVQTWTKFFALSCLLAIFIDPLFFFLIKVQEQNKCIMI 245

Query: 245 NWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKF 304
           +WP+T   VA RS+TD I+ +N+LLQFRLAYV+ ES VVGAG+LV  PK+IALHY+KGKF
Sbjct: 246 DWPMTKAFVAVRSVTDVIFTMNILLQFRLAYVARESTVVGAGQLVSHPKKIALHYLKGKF 305

Query: 305 FVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQSPS 364
           F+DL +V+PLPQI++  ++P  LG+SGANYAKNLLR AV  QYIP+LYR LP LAGQ+P+
Sbjct: 306 FLDLFIVMPLPQILILWIIPAHLGASGANYAKNLLRAAVLFQYIPKLYRLLPFLAGQTPT 365

Query: 365 GFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYIDCG 424
           GF+FE+AWANFVINLLTFMLAGH+VGS WYL GLQ VN+C R+AC   G R+C   IDCG
Sbjct: 366 GFIFESAWANFVINLLTFMLAGHVVGSCWYLFGLQRVNQCLRNACGNFG-RECQDLIDCG 425

Query: 425 HGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFWGF 484
           +G+ +       R  W +N +A ACF ++ F YGIYL+AVNLT  +++ TRY+YSLFWGF
Sbjct: 426 NGNSSVL----VRATWKDNASANACFQEDGFPYGIYLKAVNLTNHSNLFTRYSYSLFWGF 485

Query: 485 QQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRD 544
           QQISTLAGNQVPSY+  EV FTMGIIGLGLLLFALLIGNMQNFLQALG+R LEM+LRRRD
Sbjct: 486 QQISTLAGNQVPSYFLGEVFFTMGIIGLGLLLFALLIGNMQNFLQALGKRNLEMTLRRRD 545

Query: 545 VEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNKV 604
           VEQWM HRRLP+ +RR+VR+AER+NWA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ KV
Sbjct: 546 VEQWMSHRRLPDGIRRRVREAERFNWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKKV 605

Query: 605 RIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVPL 664
           RIF+LMD EPILDAIRERL+Q+TYI  S V   G ++EKMVFIVRG+MES   DG V+PL
Sbjct: 606 RIFSLMD-EPILDAIRERLKQRTYIGSSTVLHRGGLVEKMVFIVRGEMESIGEDGSVLPL 665

Query: 665 SEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVT 724
            EGDVCGEELLTWCLE SS+N D  R+++P+  L+S+R VRC+TNVEAFSL  ADLE+VT
Sbjct: 666 YEGDVCGEELLTWCLERSSVNPDGTRIRMPSKGLLSSRNVRCVTNVEAFSLSVADLEDVT 725

Query: 725 SMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           S+FSRFLR+ RVQGAIRY+SPYWR  A  +IQVAWRYR++RL R  T +S+
Sbjct: 726 SLFSRFLRSHRVQGAIRYDSPYWRLRAARQIQVAWRYRRRRLHRLCTPQSS 761

BLAST of MS018834.1 vs. ExPASy Swiss-Prot
Match: Q9LDR2 (Putative cyclic nucleotide-gated ion channel 19 OS=Arabidopsis thaliana OX=3702 GN=CNGC19 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 1.8e-227
Identity = 418/747 (55.96%), Postives = 536/747 (71.75%), Query Frame = 0

Query: 30  SFASLTRSSSLSIPTTSSGLYGNEANLVGHTGPLRSDRKSSFMV--SGSTYTGRKPERLS 89
           +F S  RS SLS P+ S   + N    +G+TGPLR+ R    +V  SG  ++ R+ E L 
Sbjct: 6   TFTSRNRSVSLSNPSFSIDGFDNSTVTLGYTGPLRTQRIRPPLVQMSGPIHSTRRTEPLF 65

Query: 90  QSNPGVPESKTAEPLADKFPSFKAKDESDWSIHNYAGRNEHLIKSGQLGMCSDPFCITCP 149
             +P   ES  +    D  P             ++  +N +L++SGQLGMC+DP+C TCP
Sbjct: 66  SPSP--QESPDSSSTVDVPPE-----------DDFVFKNANLLRSGQLGMCNDPYCTTCP 125

Query: 150 T-YNLKASKHKSLRMSGIFDHKFQSDFFGDGKGWAGKFRTFWSFLLPYIPGVMNPHAKVV 209
           + YN +A++  + R+S     +F++  +GD +GWA +F    S +   +PG+MNPH+K V
Sbjct: 126 SYYNRQAAQLHTSRVSA---SRFRTVLYGDARGWAKRFA---SSVRRCLPGIMNPHSKFV 185

Query: 210 QQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVINWPLTTTLVAFRSLTDFIYLLNML 269
           Q W +    S LVAIF+DPLFFFLL +++D KCI I+W  T  LV+ RS+TD I+ +N+L
Sbjct: 186 QVWTRVLAFSSLVAIFIDPLFFFLLLIQQDNKCIAIDWRATKVLVSLRSITDLIFFINIL 245

Query: 270 LQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQIIVFLVLPKSLG 329
           LQFRLAYV+PES +VGAG+LVD P++IA HY +GKF +D+ +V P+PQI++  ++P  LG
Sbjct: 246 LQFRLAYVAPESRIVGAGQLVDHPRKIARHYFRGKFLLDMFIVFPIPQIMILRIIPLHLG 305

Query: 330 SSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQSPSGFVFETAWANFVINLLTFMLAGHI 389
           +      K +LR  V  QYIP+LYR LPLLAGQ+ +GF+FE+AWANFVINLLTFMLAGH 
Sbjct: 306 TRREESEKQILRATVLFQYIPKLYRLLPLLAGQTSTGFIFESAWANFVINLLTFMLAGHA 365

Query: 390 VGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYIDCGHGDEAWSPDHEPRRKWGENGNAKA 449
           VGS WYL  LQ V +C  +A N   + +    IDC  G  A       R  W +N +  A
Sbjct: 366 VGSCWYLSALQRVKKCMLNAWN-ISADERRNLIDCARGSYA---SKSQRDLWRDNASVNA 425

Query: 450 CFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFWGFQQISTLAGNQVPSYYEWEVLFTMG 509
           CF +  + YGIYL+AVNLT ++S  TR++YSL+WGFQQISTLAGN  PSY   EV FTMG
Sbjct: 426 CFQENGYTYGIYLKAVNLTNESSFFTRFSYSLYWGFQQISTLAGNLSPSYSVGEVFFTMG 485

Query: 510 IIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWMEHRRLPENLRRQVRQAERY 569
           IIGLGLLLFA LIGNM NFLQ+L RRR+EM LR+RDVEQWM HRRLPE++R++VR+ ERY
Sbjct: 486 IIGLGLLLFARLIGNMHNFLQSLDRRRMEMMLRKRDVEQWMSHRRLPEDIRKRVREVERY 545

Query: 570 NWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNKVRIFALMDYEPILDAIRERLRQKTY 629
            WA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ KVRIF+LMD E +LD+IRERL+Q+TY
Sbjct: 546 TWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKKVRIFSLMD-ESVLDSIRERLKQRTY 605

Query: 630 IEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVPLSEGDVCGEELLTWCLEHSSMNRDM 689
           I  S V     ++EKMVFIVRG+MES   DG V+PLSEGDVCGEELLTWCL  SS+N D 
Sbjct: 606 IRSSTVLHHRGLVEKMVFIVRGEMESIGEDGSVLPLSEGDVCGEELLTWCL--SSINPDG 665

Query: 690 KRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVTSMFSRFLRNPRVQGAIRYESPYWR 749
            R+++P   LVSNR VRC+TNVEAFSL  ADLE+VTS+FSRFLR+ RVQGAIRYESPYWR
Sbjct: 666 TRIKMPPKGLVSNRNVRCVTNVEAFSLSVADLEDVTSLFSRFLRSHRVQGAIRYESPYWR 725

Query: 750 YLATTRIQVAWRYRKKRLSRAQTSRSN 773
             A  +IQVAWRYRK++L R  T+ SN
Sbjct: 726 LRAAMQIQVAWRYRKRQLQRLNTAHSN 726

BLAST of MS018834.1 vs. ExPASy Swiss-Prot
Match: Q9FXH6 (Putative cyclic nucleotide-gated ion channel 8 OS=Arabidopsis thaliana OX=3702 GN=CNGC8 PE=3 SV=2)

HSP 1 Score: 406.8 bits (1044), Expect = 5.6e-112
Identity = 252/580 (43.45%), Postives = 340/580 (58.62%), Query Frame = 0

Query: 198 VMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVINWPLTTTLVAFRSLT 257
           + +P  K +  WN+ FVISC++A+ +DPLFF+L  V   K CI I+  L  T    R++ 
Sbjct: 97  IFDPQDKTLLLWNRMFVISCILAVSVDPLFFYLPIVDNSKNCIGIDSKLAVTTTTLRTII 156

Query: 258 DFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQIIV 317
           D  YL  M LQFR AY++P S V G GELV  P +IA  Y+   F VD L VLPLPQI V
Sbjct: 157 DVFYLTRMALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFIVDFLAVLPLPQIAV 216

Query: 318 FLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAG-QSPSGFVFETAWANFVIN 377
           +  L  S G+      K  L + V  QYIPR  RF+PL +  +  +G   E AWA     
Sbjct: 217 WKFLHGSKGTD-VLPTKQALLHIVITQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAYY 276

Query: 378 LLTFMLAGHIVGSFWYLLGLQGVNRCFRDACN-KTGSRDCLQYIDCGHGDEAWSPDHEPR 437
           LL +MLA HI G+FWY+L ++  + C R AC  +   + C+Q + C  G +  S      
Sbjct: 277 LLWYMLASHITGAFWYMLSVERNDTCLRSACKVQPDPKVCVQILYC--GSKLMSSRETDW 336

Query: 438 RKWGENGNAKACFAKED---FDYGIYLQAVN--LTTKNSIITRYTYSLFWGFQQISTLAG 497
            K   +     C AK D   F+YGIY QAV+  + +  +  +++ Y L+WG Q +STL  
Sbjct: 337 IKSVPDLFKNNCSAKSDESKFNYGIYSQAVSSGIVSSTTFFSKFCYCLWWGLQNLSTLGQ 396

Query: 498 NQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWMEHR 557
               S Y  EVLF++ I   GLLLFALLIGNMQ +LQ+L  R  EM ++RRD EQWM HR
Sbjct: 397 GLQTSTYPGEVLFSIAIAVAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWMHHR 456

Query: 558 RLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMD 617
            LP+NLR +VR+ ++Y W  TRGV+EE I ++LP+DL+R+I+RHL    V +V +FA MD
Sbjct: 457 SLPQNLRERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFANMD 516

Query: 618 YEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVP------LS 677
            E +LDAI ERL+   Y E + +   GD + +M+FI+RG++ES   DG          L 
Sbjct: 517 -ERLLDAICERLKPSLYTESTYIVREGDPVNEMLFIIRGRLESVTTDGGRSGFFNRGLLK 576

Query: 678 EGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVTS 737
           EGD CGEELLTW L+  + +       +P+    S RTV+ LT VEAF+L A +L+ V S
Sbjct: 577 EGDFCGEELLTWALDPKAGS------NLPS----STRTVKALTEVEAFALEAEELKFVAS 636

Query: 738 MFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKR 763
            F R L + +VQ   R+ S  WR  A   IQ AWR   +R
Sbjct: 637 QFRR-LHSRQVQQTFRFYSQQWRTWAACFIQAAWRRHLRR 661

BLAST of MS018834.1 vs. ExPASy Swiss-Prot
Match: Q9S9N5 (Putative cyclic nucleotide-gated ion channel 7 OS=Arabidopsis thaliana OX=3702 GN=CNGC7 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 4.8e-111
Identity = 254/583 (43.57%), Postives = 345/583 (59.18%), Query Frame = 0

Query: 198 VMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAV-KEDKKCIVINWPLTTTLVAFRSL 257
           + +P  K +  WN+ FVISC++A+ +DPLFF+L  V      CI I+  L  T    R++
Sbjct: 90  IFDPQDKTLLVWNRLFVISCILAVSVDPLFFYLPIVDNSGSSCIGIDTKLAVTTTTLRTI 149

Query: 258 TDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQII 317
            D  YL  M LQFR AY++P S V G GELV  P +IA  Y+   F VD L VLPLPQI 
Sbjct: 150 VDVFYLTRMALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFVVDFLAVLPLPQIA 209

Query: 318 VFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAG-QSPSGFVFETAWANFVI 377
           V+  L  S GS      K  L N V VQYIPR  RF+PL +  +  +G   E AWA    
Sbjct: 210 VWKFLHGSKGSD-VLPTKTALLNIVIVQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAY 269

Query: 378 NLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACN-KTGSRDCLQYIDCG-----HGDEAWS 437
            LL +MLA HI G+FWY+L ++  + C+R AC  +   R C+Q + CG      G+  W 
Sbjct: 270 YLLWYMLASHITGAFWYMLSVERNDTCWRFACKVQPDPRLCVQILYCGTKFVSSGETEWI 329

Query: 438 PDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVN--LTTKNSIITRYTYSLFWGFQQIST 497
                  K   N +AKA  +K  F+YGIY QA++  + +  +  +++ Y L+WG Q +ST
Sbjct: 330 KTVPELLK--SNCSAKADDSK--FNYGIYGQAISSGIVSSTTFFSKFCYCLWWGLQNLST 389

Query: 498 LAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWM 557
           L      S +  EVLF++ I   GLLLFALLIGNMQ +LQ+L  R  EM ++RRD EQWM
Sbjct: 390 LGQGLQTSTFPGEVLFSIAIAIAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWM 449

Query: 558 EHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFA 617
            HR LP+NLR +VR+ ++Y W  TRGV+EE I ++LP+DL+R+I+RHL    V +V +FA
Sbjct: 450 HHRSLPQNLRERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFA 509

Query: 618 LMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVP----- 677
            MD E +LDAI ERL+   + E + +   GD + +M+FI+RG++ES   DG         
Sbjct: 510 NMD-ERLLDAICERLKPSLFTESTYIVREGDPVNEMMFIIRGRLESVTTDGGRSGFFNRG 569

Query: 678 -LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEE 737
            L EGD CGEELLTW L+  + +       +P+    S RTV+ LT VEAF+L A +L+ 
Sbjct: 570 LLKEGDFCGEELLTWALDPKAGS------NLPS----STRTVKALTEVEAFALEAEELKF 629

Query: 738 VTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKR 763
           V S F R L + +VQ   R+ S  WR  A+  IQ AWR   +R
Sbjct: 630 VASQFRR-LHSRQVQQTFRFYSQQWRTWASCFIQAAWRRYSRR 655

BLAST of MS018834.1 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 6.9e-110
Identity = 233/592 (39.36%), Postives = 342/592 (57.77%), Query Frame = 0

Query: 198 VMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVINWPLTTTLVAFRSLT 257
           +++P    +Q+WNK FV++C++A+ LDPLFF++  + + KKC+ I+  +  T    RS T
Sbjct: 84  ILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFT 143

Query: 258 DFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQIIV 317
           D  Y+L+++ QFR  +++P S V G G LV+  +EIA  Y+   F +D+L VLPLPQ+++
Sbjct: 144 DVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVI 203

Query: 318 FLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS-PSGFVFETAWANFVIN 377
            +++P   GSS  N  KN+L+  V  QYIPR  R  PL    +  SG + ETAWA    N
Sbjct: 204 LIIIPHMRGSSSLN-TKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFN 263

Query: 378 LLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYIDCGHGDEAWSPDHEPRR 437
           L  +MLA H+ G+FWYL  ++    C++ AC +       + + C           +P  
Sbjct: 264 LFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPCISKLLYC-----------DPET 323

Query: 438 KWGENGNAKACFAKED----FDYGIYLQAV--NLTTKNSIITRYTYSLFWGFQQISTLAG 497
             G     ++C  +      FD+GI+L A+   +        ++ Y  +WG Q +S+L  
Sbjct: 324 AGGNAFLNESCPIQTPNTTLFDFGIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQ 383

Query: 498 NQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWMEHR 557
           N   S Y WE+ F + I   GL+LF+ LIGNMQ +LQ+   R  EM ++RRD EQWM HR
Sbjct: 384 NLKTSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHR 443

Query: 558 RLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMD 617
            LPENLR+++R+ E+Y W  TRGV+EE +  NLP+DL+R+I+RHL    + +V +F  MD
Sbjct: 444 LLPENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMD 503

Query: 618 YEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKM-----ESRVDGIVVP--LS 677
            E +LDA+ +RL+   Y E S +   GD +++M+FI+RGK+          G +    L 
Sbjct: 504 -EQLLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLG 563

Query: 678 EGDVCGEELLTWCLE-HSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVT 737
            GD CGEELLTW L+ HSS N       +P    +S RTVR L  VEAF+L+A DL+ V 
Sbjct: 564 AGDFCGEELLTWALDPHSSSN-------LP----ISTRTVRALMEVEAFALKADDLKFVA 623

Query: 738 SMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAW-RYRKKRLSRAQTSRSN 773
           S F R L + +++   RY S  W+  A   IQ AW RY KK+L  +     N
Sbjct: 624 SQFRR-LHSKQLRHTFRYYSQQWKTWAACFIQAAWRRYIKKKLEESLKEEEN 650

BLAST of MS018834.1 vs. ExPASy TrEMBL
Match: A0A6J1C626 (probable cyclic nucleotide-gated ion channel 20, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111008737 PE=4 SV=1)

HSP 1 Score: 1553.5 bits (4021), Expect = 0.0e+00
Identity = 772/772 (100.00%), Postives = 772/772 (100.00%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT
Sbjct: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH
Sbjct: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW
Sbjct: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI
Sbjct: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG
Sbjct: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
           KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS
Sbjct: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID
Sbjct: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420

Query: 421 CGHGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFW 480
           CGHGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFW
Sbjct: 421 CGHGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFW 480

Query: 481 GFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRR 540
           GFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRR 540

Query: 541 RDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600
           RDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 601 KVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVDGIVVP 660
           KVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVDGIVVP
Sbjct: 601 KVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVDGIVVP 660

Query: 661 LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEV 720
           LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEV
Sbjct: 661 LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEV 720

Query: 721 TSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           TSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN
Sbjct: 721 TSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 772

BLAST of MS018834.1 vs. ExPASy TrEMBL
Match: A0A1S3ATK8 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103504623 PE=4 SV=1)

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 667/775 (86.06%), Postives = 713/775 (92.00%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPP+ DEQ+ SYFPS+ASL RSSSLSIP  SSG+Y +EANLVGHT
Sbjct: 1   MAAFEKDDIPMLSNTDPPIFDEQMDSYFPSYASLGRSSSLSIPANSSGMYSSEANLVGHT 60

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLR +R SSF VSGS  T  KP++LSQS PGV ESKTAEPLA+KFPSFK  D+ DWSIH
Sbjct: 61  GPLRIER-SSFAVSGSKNTDSKPKKLSQSKPGVTESKTAEPLAEKFPSFKIVDDPDWSIH 120

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLG+C+DPFCITCPTYN K S+ KS R+SGIFD KF SD +G GKGW
Sbjct: 121 NYAGRNEHLIKSGQLGLCNDPFCITCPTYNSKVSQQKSSRISGIFDAKFHSDLYGGGKGW 180

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKF++FW FLL YIPGVMNPHAKVVQQWNKFFVISCL+AIFLDPLFFFLL+V+E  KCI
Sbjct: 181 AGKFQSFWCFLLSYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFLLSVQEKNKCI 240

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VI+WPLTTTLV FRS+TDFIY L+MLLQFRLAYV+PES VVGAGELVD PK+IA++Y+KG
Sbjct: 241 VIDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPESRVVGAGELVDHPKKIAMNYLKG 300

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+DLLVVLPLPQII+FLVLPKSLGSSGANYAKNLLR AVTVQYIPRLYRFLPLLAGQS
Sbjct: 301 NFFIDLLVVLPLPQIIIFLVLPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQS 360

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQ V RCFRDACNKTG+R CL+YID
Sbjct: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQRVTRCFRDACNKTGNRSCLEYID 420

Query: 421 CGHGDEAWSPDHEPRR-KWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDE+ S   +P R  W  N NA ACF+ E F YGIYLQAVNLTT+ SI+TRYTYSLF
Sbjct: 421 CGHGDESRSQFLDPHRDSWKHNENATACFSNEGFGYGIYLQAVNLTTEKSILTRYTYSLF 480

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR
Sbjct: 481 WGFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQ+NIRRHLF+FV
Sbjct: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQKNIRRHLFKFV 600

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFS+GD IEKMVFIVRGKMESRVD  GI
Sbjct: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDTIEKMVFIVRGKMESRVDGNGI 660

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+N+DMKR QVPAPRLVSNRTVRCL+NVEAFSLRAADL
Sbjct: 661 VVPLSEGDVCGEELLTWCLEHSSINKDMKRQQVPAPRLVSNRTVRCLSNVEAFSLRAADL 720

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA  RIQVAWRYRK+RLSR QTS+SN
Sbjct: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAAMRIQVAWRYRKRRLSRVQTSQSN 774

BLAST of MS018834.1 vs. ExPASy TrEMBL
Match: A0A5A7SXH8 (Putative cyclic nucleotide-gated ion channel 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00360 PE=4 SV=1)

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 667/775 (86.06%), Postives = 713/775 (92.00%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPP+ DEQ+ SYFPS+ASL RSSSLSIP  SSG+Y +EANLVGHT
Sbjct: 91  MAAFEKDDIPMLSNTDPPIFDEQMDSYFPSYASLGRSSSLSIPANSSGMYSSEANLVGHT 150

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLR +R SSF VSGS  T  KP++LSQS PGV ESKTAEPLA+KFPSFK  D+ DWSIH
Sbjct: 151 GPLRIER-SSFAVSGSKNTDSKPKKLSQSKPGVTESKTAEPLAEKFPSFKIVDDPDWSIH 210

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLG+C+DPFCITCPTYN K S+ KS R+SGIFD KF SD +G GKGW
Sbjct: 211 NYAGRNEHLIKSGQLGLCNDPFCITCPTYNSKVSQQKSSRISGIFDAKFHSDLYGGGKGW 270

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKF++FW FLL YIPGVMNPHAKVVQQWNKFFVISCL+AIFLDPLFFFLL+V+E  KCI
Sbjct: 271 AGKFQSFWCFLLSYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFLLSVQEKNKCI 330

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VI+WPLTTTLV FRS+TDFIY L+MLLQFRLAYV+PES VVGAGELVD PK+IA++Y+KG
Sbjct: 331 VIDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPESRVVGAGELVDHPKKIAMNYLKG 390

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+DLLVVLPLPQII+FLVLPKSLGSSGANYAKNLLR AVTVQYIPRLYRFLPLLAGQS
Sbjct: 391 NFFIDLLVVLPLPQIIIFLVLPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQS 450

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQ V RCFRDACNKTG+R CL+YID
Sbjct: 451 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQRVTRCFRDACNKTGNRSCLEYID 510

Query: 421 CGHGDEAWSPDHEPRR-KWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDE+ S   +P R  W  N NA ACF+ E F YGIYLQAVNLTT+ SI+TRYTYSLF
Sbjct: 511 CGHGDESRSQFLDPHRDSWKHNENATACFSNEGFGYGIYLQAVNLTTEKSILTRYTYSLF 570

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR
Sbjct: 571 WGFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 630

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQ+NIRRHLF+FV
Sbjct: 631 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQKNIRRHLFKFV 690

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFS+GD IEKMVFIVRGKMESRVD  GI
Sbjct: 691 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDTIEKMVFIVRGKMESRVDGNGI 750

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+N+DMKR QVPAPRLVSNRTVRCL+NVEAFSLRAADL
Sbjct: 751 VVPLSEGDVCGEELLTWCLEHSSINKDMKRQQVPAPRLVSNRTVRCLSNVEAFSLRAADL 810

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA  RIQVAWRYRK+RLSR QTS+SN
Sbjct: 811 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAAMRIQVAWRYRKRRLSRVQTSQSN 864

BLAST of MS018834.1 vs. ExPASy TrEMBL
Match: A0A1S3CT38 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103504623 PE=4 SV=1)

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 667/775 (86.06%), Postives = 713/775 (92.00%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MAAFEKDDIPMLSNTDPP+ DEQ+ SYFPS+ASL RSSSLSIP  SSG+Y +EANLVGHT
Sbjct: 25  MAAFEKDDIPMLSNTDPPIFDEQMDSYFPSYASLGRSSSLSIPANSSGMYSSEANLVGHT 84

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLR +R SSF VSGS  T  KP++LSQS PGV ESKTAEPLA+KFPSFK  D+ DWSIH
Sbjct: 85  GPLRIER-SSFAVSGSKNTDSKPKKLSQSKPGVTESKTAEPLAEKFPSFKIVDDPDWSIH 144

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           NYAGRNEHLIKSGQLG+C+DPFCITCPTYN K S+ KS R+SGIFD KF SD +G GKGW
Sbjct: 145 NYAGRNEHLIKSGQLGLCNDPFCITCPTYNSKVSQQKSSRISGIFDAKFHSDLYGGGKGW 204

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           AGKF++FW FLL YIPGVMNPHAKVVQQWNKFFVISCL+AIFLDPLFFFLL+V+E  KCI
Sbjct: 205 AGKFQSFWCFLLSYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFLLSVQEKNKCI 264

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
           VI+WPLTTTLV FRS+TDFIY L+MLLQFRLAYV+PES VVGAGELVD PK+IA++Y+KG
Sbjct: 265 VIDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPESRVVGAGELVDHPKKIAMNYLKG 324

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+DLLVVLPLPQII+FLVLPKSLGSSGANYAKNLLR AVTVQYIPRLYRFLPLLAGQS
Sbjct: 325 NFFIDLLVVLPLPQIIIFLVLPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQS 384

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQ V RCFRDACNKTG+R CL+YID
Sbjct: 385 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQRVTRCFRDACNKTGNRSCLEYID 444

Query: 421 CGHGDEAWSPDHEPRR-KWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDE+ S   +P R  W  N NA ACF+ E F YGIYLQAVNLTT+ SI+TRYTYSLF
Sbjct: 445 CGHGDESRSQFLDPHRDSWKHNENATACFSNEGFGYGIYLQAVNLTTEKSILTRYTYSLF 504

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR
Sbjct: 505 WGFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 564

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQ+NIRRHLF+FV
Sbjct: 565 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQKNIRRHLFKFV 624

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFS+GD IEKMVFIVRGKMESRVD  GI
Sbjct: 625 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDTIEKMVFIVRGKMESRVDGNGI 684

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+N+DMKR QVPAPRLVSNRTVRCL+NVEAFSLRAADL
Sbjct: 685 VVPLSEGDVCGEELLTWCLEHSSINKDMKRQQVPAPRLVSNRTVRCLSNVEAFSLRAADL 744

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA  RIQVAWRYRK+RLSR QTS+SN
Sbjct: 745 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAAMRIQVAWRYRKRRLSRVQTSQSN 798

BLAST of MS018834.1 vs. ExPASy TrEMBL
Match: A0A6J1KKW9 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495122 PE=4 SV=1)

HSP 1 Score: 1302.0 bits (3368), Expect = 0.0e+00
Identity = 648/775 (83.61%), Postives = 707/775 (91.23%), Query Frame = 0

Query: 1   MAAFEKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHT 60
           MA+FEKDD PMLSNT PP+LDEQ+GSYFPS+ASL RSSSLSIP TSSG+Y ++A LVG+T
Sbjct: 1   MASFEKDDTPMLSNTGPPLLDEQLGSYFPSYASLARSSSLSIP-TSSGIYVSDAKLVGYT 60

Query: 61  GPLRSDRKSSFMVSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIH 120
           GPLR +RKS F+  GS YT RKPE+ SQS+PGV ESKTAEPL DKFPSFK  DESDW+I 
Sbjct: 61  GPLRIERKSPFLEIGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDESDWAIQ 120

Query: 121 NYAGRNEHLIKSGQLGMCSDPFCITCPTYNLKASKHKSLRMSGIFDHKFQSDFFGDGKGW 180
           ++AGRNEHLIKSGQLG+C+DPFCITCPTYN K+SK KS RM GIFD +F  D +G+GKGW
Sbjct: 121 HFAGRNEHLIKSGQLGVCNDPFCITCPTYNSKSSKQKSSRMRGIFDPEFHKDLYGEGKGW 180

Query: 181 AGKFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCI 240
           A KFR+ W FLLPYIPGVMNPHAKVVQQWNKFFVISCL+AIFLDPLFFF+LAV   KKCI
Sbjct: 181 AVKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCI 240

Query: 241 VINWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKG 300
            INWP+TTTLV FRS+TDF+Y L+MLLQFRLAYV+PE+ VVGAGELVD PK+IA +Y+KG
Sbjct: 241 FINWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKG 300

Query: 301 KFFVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS 360
            FF+D+LVVLPLPQIIVFL+LP SLG+SGAN+AKNLLR AV  QYIPRLYRFLPLLAGQS
Sbjct: 301 NFFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVIAQYIPRLYRFLPLLAGQS 360

Query: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYID 420
           PSGFVFETAWANFVINLLTFMLAGHIVGS WYL GLQGVNRCFRDACN+TG+  CL YID
Sbjct: 361 PSGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGNTSCLDYID 420

Query: 421 CGHGDEAWSPDHEPRR-KWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLF 480
           CGHGDEA +   + RR +W  N NA ACFAKE FDYGIYLQAVNLTT+++++TRYTYSLF
Sbjct: 421 CGHGDEAGNHSPDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLF 480

Query: 481 WGFQQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLR 540
           WGFQQISTLAGNQVPSYY +EVLFTMGIIGLGLLLFALLIGNMQ FLQALGRRRLEMSLR
Sbjct: 481 WGFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLR 540

Query: 541 RRDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600
           RRDVEQWMEHRRLPE+LRR+VRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV
Sbjct: 541 RRDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFV 600

Query: 601 NKVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMESRVD--GI 660
           NKVRIFALMDYEPILDAIRERLRQ+TYIEGSEVF++GDIIEKMVFIVRGKMESRVD  GI
Sbjct: 601 NKVRIFALMDYEPILDAIRERLRQRTYIEGSEVFTAGDIIEKMVFIVRGKMESRVDGNGI 660

Query: 661 VVPLSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADL 720
           VVPLSEGDVCGEELLTWCLEHSS+NRDMKR Q+PA +LVSNRTV+C TNVEAFSLRAADL
Sbjct: 661 VVPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADL 720

Query: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           EEVTSMFSRFLRNPRVQGAIRYESPYWRYLA TRIQVAWRYRK+RLSRAQTS SN
Sbjct: 721 EEVTSMFSRFLRNPRVQGAIRYESPYWRYLAATRIQVAWRYRKRRLSRAQTSHSN 774

BLAST of MS018834.1 vs. TAIR 10
Match: AT3G17700.1 (cyclic nucleotide-binding transporter 1 )

HSP 1 Score: 911.4 bits (2354), Expect = 5.0e-265
Identity = 468/771 (60.70%), Postives = 592/771 (76.78%), Query Frame = 0

Query: 5   EKDDIPMLSNTDPPMLDEQVGSYFPSFASLTRSSSLSIPTTSSGLYGNEANLVGHTGPLR 64
           E DDIPML     P+ D    +   +F S +RS SLS PT+S   +     ++G+TGPLR
Sbjct: 6   ENDDIPML-----PISDPSSRTRARAFTSRSRSVSLSNPTSSIEGFDTSTVVLGYTGPLR 65

Query: 65  SDRKSSFM-VSGSTYTGRKPERLSQSNPGVPESKTAEPLADKFPSFKAKDESDWSIHNYA 124
           + R+   + +SG   + RK E L   +P   +S       +++PSF A +  + S   + 
Sbjct: 66  TQRRPPLVQMSGPLTSTRKHEPLFLPHPS-SDSVGVSSQPERYPSFAALEHKNSSEDEFV 125

Query: 125 GRNEHLIKSGQLGMCSDPFCITCPT-YNLKASKHKSLRMSGIFDHKFQSDFFGDGKGWAG 184
            ++ +L++SGQLGMC+DP+C TCP+ YN KA++  + R+S +FD  F +  + D KGWA 
Sbjct: 126 LKHANLLRSGQLGMCNDPYCTTCPSYYNRKAAQIPTSRVSALFDSTFHNALYDDAKGWA- 185

Query: 185 KFRTFWSFLLPYIPGVMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVI 244
             R F S +  Y+PG+MNPHAK VQ W KFF +SCL+AIF+DPLFFFL+ V+E  KCI+I
Sbjct: 186 --RRFASSVNRYLPGIMNPHAKEVQTWTKFFALSCLLAIFIDPLFFFLIKVQEQNKCIMI 245

Query: 245 NWPLTTTLVAFRSLTDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKF 304
           +WP+T   VA RS+TD I+ +N+LLQFRLAYV+ ES VVGAG+LV  PK+IALHY+KGKF
Sbjct: 246 DWPMTKAFVAVRSVTDVIFTMNILLQFRLAYVARESTVVGAGQLVSHPKKIALHYLKGKF 305

Query: 305 FVDLLVVLPLPQIIVFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQSPS 364
           F+DL +V+PLPQI++  ++P  LG+SGANYAKNLLR AV  QYIP+LYR LP LAGQ+P+
Sbjct: 306 FLDLFIVMPLPQILILWIIPAHLGASGANYAKNLLRAAVLFQYIPKLYRLLPFLAGQTPT 365

Query: 365 GFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYIDCG 424
           GF+FE+AWANFVINLLTFMLAGH+VGS WYL GLQ VN+C R+AC   G R+C   IDCG
Sbjct: 366 GFIFESAWANFVINLLTFMLAGHVVGSCWYLFGLQRVNQCLRNACGNFG-RECQDLIDCG 425

Query: 425 HGDEAWSPDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFWGF 484
           +G+ +       R  W +N +A ACF ++ F YGIYL+AVNLT  +++ TRY+YSLFWGF
Sbjct: 426 NGNSSVL----VRATWKDNASANACFQEDGFPYGIYLKAVNLTNHSNLFTRYSYSLFWGF 485

Query: 485 QQISTLAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRD 544
           QQISTLAGNQVPSY+  EV FTMGIIGLGLLLFALLIGNMQNFLQALG+R LEM+LRRRD
Sbjct: 486 QQISTLAGNQVPSYFLGEVFFTMGIIGLGLLLFALLIGNMQNFLQALGKRNLEMTLRRRD 545

Query: 545 VEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNKV 604
           VEQWM HRRLP+ +RR+VR+AER+NWA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ KV
Sbjct: 546 VEQWMSHRRLPDGIRRRVREAERFNWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKKV 605

Query: 605 RIFALMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVPL 664
           RIF+LMD EPILDAIRERL+Q+TYI  S V   G ++EKMVFIVRG+MES   DG V+PL
Sbjct: 606 RIFSLMD-EPILDAIRERLKQRTYIGSSTVLHRGGLVEKMVFIVRGEMESIGEDGSVLPL 665

Query: 665 SEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVT 724
            EGDVCGEELLTWCLE SS+N D  R+++P+  L+S+R VRC+TNVEAFSL  ADLE+VT
Sbjct: 666 YEGDVCGEELLTWCLERSSVNPDGTRIRMPSKGLLSSRNVRCVTNVEAFSLSVADLEDVT 725

Query: 725 SMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKRLSRAQTSRSN 773
           S+FSRFLR+ RVQGAIRY+SPYWR  A  +IQVAWRYR++RL R  T +S+
Sbjct: 726 SLFSRFLRSHRVQGAIRYDSPYWRLRAARQIQVAWRYRRRRLHRLCTPQSS 761

BLAST of MS018834.1 vs. TAIR 10
Match: AT3G17690.1 (cyclic nucleotide gated channel 19 )

HSP 1 Score: 790.4 bits (2040), Expect = 1.3e-228
Identity = 418/747 (55.96%), Postives = 536/747 (71.75%), Query Frame = 0

Query: 30  SFASLTRSSSLSIPTTSSGLYGNEANLVGHTGPLRSDRKSSFMV--SGSTYTGRKPERLS 89
           +F S  RS SLS P+ S   + N    +G+TGPLR+ R    +V  SG  ++ R+ E L 
Sbjct: 6   TFTSRNRSVSLSNPSFSIDGFDNSTVTLGYTGPLRTQRIRPPLVQMSGPIHSTRRTEPLF 65

Query: 90  QSNPGVPESKTAEPLADKFPSFKAKDESDWSIHNYAGRNEHLIKSGQLGMCSDPFCITCP 149
             +P   ES  +    D  P             ++  +N +L++SGQLGMC+DP+C TCP
Sbjct: 66  SPSP--QESPDSSSTVDVPPE-----------DDFVFKNANLLRSGQLGMCNDPYCTTCP 125

Query: 150 T-YNLKASKHKSLRMSGIFDHKFQSDFFGDGKGWAGKFRTFWSFLLPYIPGVMNPHAKVV 209
           + YN +A++  + R+S     +F++  +GD +GWA +F    S +   +PG+MNPH+K V
Sbjct: 126 SYYNRQAAQLHTSRVSA---SRFRTVLYGDARGWAKRFA---SSVRRCLPGIMNPHSKFV 185

Query: 210 QQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVINWPLTTTLVAFRSLTDFIYLLNML 269
           Q W +    S LVAIF+DPLFFFLL +++D KCI I+W  T  LV+ RS+TD I+ +N+L
Sbjct: 186 QVWTRVLAFSSLVAIFIDPLFFFLLLIQQDNKCIAIDWRATKVLVSLRSITDLIFFINIL 245

Query: 270 LQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQIIVFLVLPKSLG 329
           LQFRLAYV+PES +VGAG+LVD P++IA HY +GKF +D+ +V P+PQI++  ++P  LG
Sbjct: 246 LQFRLAYVAPESRIVGAGQLVDHPRKIARHYFRGKFLLDMFIVFPIPQIMILRIIPLHLG 305

Query: 330 SSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQSPSGFVFETAWANFVINLLTFMLAGHI 389
           +      K +LR  V  QYIP+LYR LPLLAGQ+ +GF+FE+AWANFVINLLTFMLAGH 
Sbjct: 306 TRREESEKQILRATVLFQYIPKLYRLLPLLAGQTSTGFIFESAWANFVINLLTFMLAGHA 365

Query: 390 VGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYIDCGHGDEAWSPDHEPRRKWGENGNAKA 449
           VGS WYL  LQ V +C  +A N   + +    IDC  G  A       R  W +N +  A
Sbjct: 366 VGSCWYLSALQRVKKCMLNAWN-ISADERRNLIDCARGSYA---SKSQRDLWRDNASVNA 425

Query: 450 CFAKEDFDYGIYLQAVNLTTKNSIITRYTYSLFWGFQQISTLAGNQVPSYYEWEVLFTMG 509
           CF +  + YGIYL+AVNLT ++S  TR++YSL+WGFQQISTLAGN  PSY   EV FTMG
Sbjct: 426 CFQENGYTYGIYLKAVNLTNESSFFTRFSYSLYWGFQQISTLAGNLSPSYSVGEVFFTMG 485

Query: 510 IIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWMEHRRLPENLRRQVRQAERY 569
           IIGLGLLLFA LIGNM NFLQ+L RRR+EM LR+RDVEQWM HRRLPE++R++VR+ ERY
Sbjct: 486 IIGLGLLLFARLIGNMHNFLQSLDRRRMEMMLRKRDVEQWMSHRRLPEDIRKRVREVERY 545

Query: 570 NWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNKVRIFALMDYEPILDAIRERLRQKTY 629
            WA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ KVRIF+LMD E +LD+IRERL+Q+TY
Sbjct: 546 TWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKKVRIFSLMD-ESVLDSIRERLKQRTY 605

Query: 630 IEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVPLSEGDVCGEELLTWCLEHSSMNRDM 689
           I  S V     ++EKMVFIVRG+MES   DG V+PLSEGDVCGEELLTWCL  SS+N D 
Sbjct: 606 IRSSTVLHHRGLVEKMVFIVRGEMESIGEDGSVLPLSEGDVCGEELLTWCL--SSINPDG 665

Query: 690 KRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVTSMFSRFLRNPRVQGAIRYESPYWR 749
            R+++P   LVSNR VRC+TNVEAFSL  ADLE+VTS+FSRFLR+ RVQGAIRYESPYWR
Sbjct: 666 TRIKMPPKGLVSNRNVRCVTNVEAFSLSVADLEDVTSLFSRFLRSHRVQGAIRYESPYWR 725

Query: 750 YLATTRIQVAWRYRKKRLSRAQTSRSN 773
             A  +IQVAWRYRK++L R  T+ SN
Sbjct: 726 LRAAMQIQVAWRYRKRQLQRLNTAHSN 726

BLAST of MS018834.1 vs. TAIR 10
Match: AT1G19780.1 (cyclic nucleotide gated channel 8 )

HSP 1 Score: 406.8 bits (1044), Expect = 4.0e-113
Identity = 252/580 (43.45%), Postives = 340/580 (58.62%), Query Frame = 0

Query: 198 VMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVINWPLTTTLVAFRSLT 257
           + +P  K +  WN+ FVISC++A+ +DPLFF+L  V   K CI I+  L  T    R++ 
Sbjct: 97  IFDPQDKTLLLWNRMFVISCILAVSVDPLFFYLPIVDNSKNCIGIDSKLAVTTTTLRTII 156

Query: 258 DFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQIIV 317
           D  YL  M LQFR AY++P S V G GELV  P +IA  Y+   F VD L VLPLPQI V
Sbjct: 157 DVFYLTRMALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFIVDFLAVLPLPQIAV 216

Query: 318 FLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAG-QSPSGFVFETAWANFVIN 377
           +  L  S G+      K  L + V  QYIPR  RF+PL +  +  +G   E AWA     
Sbjct: 217 WKFLHGSKGTD-VLPTKQALLHIVITQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAYY 276

Query: 378 LLTFMLAGHIVGSFWYLLGLQGVNRCFRDACN-KTGSRDCLQYIDCGHGDEAWSPDHEPR 437
           LL +MLA HI G+FWY+L ++  + C R AC  +   + C+Q + C  G +  S      
Sbjct: 277 LLWYMLASHITGAFWYMLSVERNDTCLRSACKVQPDPKVCVQILYC--GSKLMSSRETDW 336

Query: 438 RKWGENGNAKACFAKED---FDYGIYLQAVN--LTTKNSIITRYTYSLFWGFQQISTLAG 497
            K   +     C AK D   F+YGIY QAV+  + +  +  +++ Y L+WG Q +STL  
Sbjct: 337 IKSVPDLFKNNCSAKSDESKFNYGIYSQAVSSGIVSSTTFFSKFCYCLWWGLQNLSTLGQ 396

Query: 498 NQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWMEHR 557
               S Y  EVLF++ I   GLLLFALLIGNMQ +LQ+L  R  EM ++RRD EQWM HR
Sbjct: 397 GLQTSTYPGEVLFSIAIAVAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWMHHR 456

Query: 558 RLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMD 617
            LP+NLR +VR+ ++Y W  TRGV+EE I ++LP+DL+R+I+RHL    V +V +FA MD
Sbjct: 457 SLPQNLRERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFANMD 516

Query: 618 YEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVP------LS 677
            E +LDAI ERL+   Y E + +   GD + +M+FI+RG++ES   DG          L 
Sbjct: 517 -ERLLDAICERLKPSLYTESTYIVREGDPVNEMLFIIRGRLESVTTDGGRSGFFNRGLLK 576

Query: 678 EGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVTS 737
           EGD CGEELLTW L+  + +       +P+    S RTV+ LT VEAF+L A +L+ V S
Sbjct: 577 EGDFCGEELLTWALDPKAGS------NLPS----STRTVKALTEVEAFALEAEELKFVAS 636

Query: 738 MFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKR 763
            F R L + +VQ   R+ S  WR  A   IQ AWR   +R
Sbjct: 637 QFRR-LHSRQVQQTFRFYSQQWRTWAACFIQAAWRRHLRR 661

BLAST of MS018834.1 vs. TAIR 10
Match: AT1G15990.1 (cyclic nucleotide gated channel 7 )

HSP 1 Score: 403.7 bits (1036), Expect = 3.4e-112
Identity = 254/583 (43.57%), Postives = 345/583 (59.18%), Query Frame = 0

Query: 198 VMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAV-KEDKKCIVINWPLTTTLVAFRSL 257
           + +P  K +  WN+ FVISC++A+ +DPLFF+L  V      CI I+  L  T    R++
Sbjct: 61  IFDPQDKTLLVWNRLFVISCILAVSVDPLFFYLPIVDNSGSSCIGIDTKLAVTTTTLRTI 120

Query: 258 TDFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQII 317
            D  YL  M LQFR AY++P S V G GELV  P +IA  Y+   F VD L VLPLPQI 
Sbjct: 121 VDVFYLTRMALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFVVDFLAVLPLPQIA 180

Query: 318 VFLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAG-QSPSGFVFETAWANFVI 377
           V+  L  S GS      K  L N V VQYIPR  RF+PL +  +  +G   E AWA    
Sbjct: 181 VWKFLHGSKGSD-VLPTKTALLNIVIVQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAY 240

Query: 378 NLLTFMLAGHIVGSFWYLLGLQGVNRCFRDACN-KTGSRDCLQYIDCG-----HGDEAWS 437
            LL +MLA HI G+FWY+L ++  + C+R AC  +   R C+Q + CG      G+  W 
Sbjct: 241 YLLWYMLASHITGAFWYMLSVERNDTCWRFACKVQPDPRLCVQILYCGTKFVSSGETEWI 300

Query: 438 PDHEPRRKWGENGNAKACFAKEDFDYGIYLQAVN--LTTKNSIITRYTYSLFWGFQQIST 497
                  K   N +AKA  +K  F+YGIY QA++  + +  +  +++ Y L+WG Q +ST
Sbjct: 301 KTVPELLK--SNCSAKADDSK--FNYGIYGQAISSGIVSSTTFFSKFCYCLWWGLQNLST 360

Query: 498 LAGNQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWM 557
           L      S +  EVLF++ I   GLLLFALLIGNMQ +LQ+L  R  EM ++RRD EQWM
Sbjct: 361 LGQGLQTSTFPGEVLFSIAIAIAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWM 420

Query: 558 EHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFA 617
            HR LP+NLR +VR+ ++Y W  TRGV+EE I ++LP+DL+R+I+RHL    V +V +FA
Sbjct: 421 HHRSLPQNLRERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFA 480

Query: 618 LMDYEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKMES-RVDGIVVP----- 677
            MD E +LDAI ERL+   + E + +   GD + +M+FI+RG++ES   DG         
Sbjct: 481 NMD-ERLLDAICERLKPSLFTESTYIVREGDPVNEMMFIIRGRLESVTTDGGRSGFFNRG 540

Query: 678 -LSEGDVCGEELLTWCLEHSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEE 737
            L EGD CGEELLTW L+  + +       +P+    S RTV+ LT VEAF+L A +L+ 
Sbjct: 541 LLKEGDFCGEELLTWALDPKAGS------NLPS----STRTVKALTEVEAFALEAEELKF 600

Query: 738 VTSMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAWRYRKKR 763
           V S F R L + +VQ   R+ S  WR  A+  IQ AWR   +R
Sbjct: 601 VASQFRR-LHSRQVQQTFRFYSQQWRTWASCFIQAAWRRYSRR 626

BLAST of MS018834.1 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 399.8 bits (1026), Expect = 4.9e-111
Identity = 233/592 (39.36%), Postives = 342/592 (57.77%), Query Frame = 0

Query: 198 VMNPHAKVVQQWNKFFVISCLVAIFLDPLFFFLLAVKEDKKCIVINWPLTTTLVAFRSLT 257
           +++P    +Q+WNK FV++C++A+ LDPLFF++  + + KKC+ I+  +  T    RS T
Sbjct: 84  ILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFT 143

Query: 258 DFIYLLNMLLQFRLAYVSPESLVVGAGELVDQPKEIALHYIKGKFFVDLLVVLPLPQIIV 317
           D  Y+L+++ QFR  +++P S V G G LV+  +EIA  Y+   F +D+L VLPLPQ+++
Sbjct: 144 DVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVI 203

Query: 318 FLVLPKSLGSSGANYAKNLLRNAVTVQYIPRLYRFLPLLAGQS-PSGFVFETAWANFVIN 377
            +++P   GSS  N  KN+L+  V  QYIPR  R  PL    +  SG + ETAWA    N
Sbjct: 204 LIIIPHMRGSSSLN-TKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFN 263

Query: 378 LLTFMLAGHIVGSFWYLLGLQGVNRCFRDACNKTGSRDCLQYIDCGHGDEAWSPDHEPRR 437
           L  +MLA H+ G+FWYL  ++    C++ AC +       + + C           +P  
Sbjct: 264 LFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPCISKLLYC-----------DPET 323

Query: 438 KWGENGNAKACFAKED----FDYGIYLQAV--NLTTKNSIITRYTYSLFWGFQQISTLAG 497
             G     ++C  +      FD+GI+L A+   +        ++ Y  +WG Q +S+L  
Sbjct: 324 AGGNAFLNESCPIQTPNTTLFDFGIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQ 383

Query: 498 NQVPSYYEWEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRRRDVEQWMEHR 557
           N   S Y WE+ F + I   GL+LF+ LIGNMQ +LQ+   R  EM ++RRD EQWM HR
Sbjct: 384 NLKTSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHR 443

Query: 558 RLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMD 617
            LPENLR+++R+ E+Y W  TRGV+EE +  NLP+DL+R+I+RHL    + +V +F  MD
Sbjct: 444 LLPENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMD 503

Query: 618 YEPILDAIRERLRQKTYIEGSEVFSSGDIIEKMVFIVRGKM-----ESRVDGIVVP--LS 677
            E +LDA+ +RL+   Y E S +   GD +++M+FI+RGK+          G +    L 
Sbjct: 504 -EQLLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLG 563

Query: 678 EGDVCGEELLTWCLE-HSSMNRDMKRLQVPAPRLVSNRTVRCLTNVEAFSLRAADLEEVT 737
            GD CGEELLTW L+ HSS N       +P    +S RTVR L  VEAF+L+A DL+ V 
Sbjct: 564 AGDFCGEELLTWALDPHSSSN-------LP----ISTRTVRALMEVEAFALKADDLKFVA 623

Query: 738 SMFSRFLRNPRVQGAIRYESPYWRYLATTRIQVAW-RYRKKRLSRAQTSRSN 773
           S F R L + +++   RY S  W+  A   IQ AW RY KK+L  +     N
Sbjct: 624 SQFRR-LHSKQLRHTFRYYSQQWKTWAACFIQAAWRRYIKKKLEESLKEEEN 650