MS018654.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS018654.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	psbQ-like protein 3, chloroplastic
Location	scaffold313: 770950 .. 771574 (+)
Sequence length	543
RNA-Seq Expression	MS018654.1
Synteny	MS018654.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAATTGAACCCAATCTCACTCCCAAGCCTCTGTAGCCGCACCAACTTCTCTTCCTCTCCCGCTGTCGTCTCCTTCCGATCGTTCAATATCGCCGCCAATTGTTCTAACGAGAACACAAACATCAGCAGAAGAATAGGGTTAGGATTAGGGCTGGGATTGACAGGTTCAATGGCGGTGGGAGCGGCAAATGCTTTGGATCTGAAATTGATCGCGCCGGGCCAGAGCTTCGAGGAGGCGGAGAGCGGAATCAGAGGCCACGCGGAGGCGCTTTTGGAGGTGAGAGGTTTGATTGAATCGGAGGCTTGGAAGGAGGCTCAGAAGAGGTTGAGAAGCAGTTCGCCGCCGCTGAAACAGGATCTACATACTATAATTGAATACAAACCGCCGGCTGAGAGGCCTCCTCTAAGGAAACTTTACTCTCTTCTCTTCAACAATATTACACGAGTAAGTAATTAATCCTCACCAGATTTTCTTCGATTCAGAATATTGATTGATGCAAATTTTGTTTGTTTTGGTGATTTTGCAGATGGATTATGCAGCGAGAGACAGAGATGCAGAACGAGTTTGGGAATGCTACGGTAACATTGTGATGGCCATTTCTGACATTTTGTCCAGAATT

mRNA sequence

ATGGCGGAATTGAACCCAATCTCACTCCCAAGCCTCTGTAGCCGCACCAACTTCTCTTCCTCTCCCGCTGTCGTCTCCTTCCGATCGTTCAATATCGCCGCCAATTGTTCTAACGAGAACACAAACATCAGCAGAAGAATAGGGTTAGGATTAGGGCTGGGATTGACAGGTTCAATGGCGGTGGGAGCGGCAAATGCTTTGGATCTGAAATTGATCGCGCCGGGCCAGAGCTTCGAGGAGGCGGAGAGCGGAATCAGAGGCCACGCGGAGGCGCTTTTGGAGGTGAGAGGTTTGATTGAATCGGAGGCTTGGAAGGAGGCTCAGAAGAGGTTGAGAAGCAGTTCGCCGCCGCTGAAACAGGATCTACATACTATAATTGAATACAAACCGCCGGCTGAGAGGCCTCCTCTAAGGAAACTTTACTCTCTTCTCTTCAACAATATTACACGAATGGATTATGCAGCGAGAGACAGAGATGCAGAACGAGTTTGGGAATGCTACGGTAACATTGTGATGGCCATTTCTGACATTTTGTCCAGAATT

Coding sequence (CDS)

ATGGCGGAATTGAACCCAATCTCACTCCCAAGCCTCTGTAGCCGCACCAACTTCTCTTCCTCTCCCGCTGTCGTCTCCTTCCGATCGTTCAATATCGCCGCCAATTGTTCTAACGAGAACACAAACATCAGCAGAAGAATAGGGTTAGGATTAGGGCTGGGATTGACAGGTTCAATGGCGGTGGGAGCGGCAAATGCTTTGGATCTGAAATTGATCGCGCCGGGCCAGAGCTTCGAGGAGGCGGAGAGCGGAATCAGAGGCCACGCGGAGGCGCTTTTGGAGGTGAGAGGTTTGATTGAATCGGAGGCTTGGAAGGAGGCTCAGAAGAGGTTGAGAAGCAGTTCGCCGCCGCTGAAACAGGATCTACATACTATAATTGAATACAAACCGCCGGCTGAGAGGCCTCCTCTAAGGAAACTTTACTCTCTTCTCTTCAACAATATTACACGAATGGATTATGCAGCGAGAGACAGAGATGCAGAACGAGTTTGGGAATGCTACGGTAACATTGTGATGGCCATTTCTGACATTTTGTCCAGAATT

Protein sequence

MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLGLGLGLTGSMAVGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSRI

Homology

[Close]

BLAST of MS018654.1 vs. NCBI nr
Match: XP_022141571.1 (psbQ-like protein 3, chloroplastic [Momordica charantia])

HSP 1 Score: 351.3 bits (900), Expect = 5.0e-93
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLGLGLGLTGSMA 60
           MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENT+ISRRIGLGLGLGLTGSMA
Sbjct: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTSISRRIGLGLGLGLTGSMA 60

Query: 61  VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQ 120
           VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQ
Sbjct: 61  VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQ 120

Query: 121 DLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSR 180
           DLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSR
Sbjct: 121 DLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSR 180

Query: 181 I 182
           I
Sbjct: 181 I 181

[Close]

BLAST of MS018654.1 vs. NCBI nr
Match: XP_022991100.1 (psbQ-like protein 3, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 255.4 bits (651), Expect = 3.8e-64
Identity = 136/185 (73.51%), Postives = 159/185 (85.95%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLG-LGLGLTGSM 60
           MAEL+PIS+ +L +R +FSSSP ++SF+ F+I A   +   +ISRR+GLG + L  + S+
Sbjct: 1   MAELSPISVSNLSNRIHFSSSPPIISFQLFSITAKRCSNCRSISRRLGLGFMALMASSSV 60

Query: 61  A---VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSP 120
           A   V   NALDLKLIAP QSFEEAESGIRGHAEA+L+VRGLIESE+WKEAQK+LRSSSP
Sbjct: 61  ASTPVKITNALDLKLIAPEQSFEEAESGIRGHAEAVLQVRGLIESESWKEAQKQLRSSSP 120

Query: 121 PLKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISD 180
            LKQDL+TIIEYKPPAERPPLRKLYSLLFNN+TRMDYAARD+DAERVW+CY NIVMAISD
Sbjct: 121 LLKQDLYTIIEYKPPAERPPLRKLYSLLFNNVTRMDYAARDKDAERVWQCYDNIVMAISD 180

Query: 181 ILSRI 182
           ILSRI
Sbjct: 181 ILSRI 185

[Close]

BLAST of MS018654.1 vs. NCBI nr
Match: XP_023551782.1 (psbQ-like protein 3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.5 bits (641), Expect = 5.4e-63
Identity = 137/191 (71.73%), Postives = 162/191 (84.82%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNF-SSSPAVVSFRSFNIAA----NCSNENTNISRRIGLGLGLGL 60
           MAEL+PIS+ +L +R +F SSSP ++S + F+I A    NC + + +ISRR+GLGL + L
Sbjct: 1   MAELSPISVSNLSNRIHFSSSSPPIISVQLFSITAKRCSNCRSISRSISRRLGLGL-IAL 60

Query: 61  TGSMAVGA-----ANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKR 120
             S +V +     ANALDLKLIAP QSFEEAESGIRGHAEA+L+VRGLIESE+WKEAQK+
Sbjct: 61  MASSSVASTPVKIANALDLKLIAPEQSFEEAESGIRGHAEAVLQVRGLIESESWKEAQKQ 120

Query: 121 LRSSSPPLKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNI 180
           LRSSSP LKQDL+TIIEYKPPAERPPLR+LYSLLFNN+TRMDYAARD+DAERVW+CY NI
Sbjct: 121 LRSSSPLLKQDLYTIIEYKPPAERPPLRRLYSLLFNNVTRMDYAARDKDAERVWQCYDNI 180

Query: 181 VMAISDILSRI 182
           VMAISDILSRI
Sbjct: 181 VMAISDILSRI 190

[Close]

BLAST of MS018654.1 vs. NCBI nr
Match: XP_038877067.1 (psbQ-like protein 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 243.0 bits (619), Expect = 1.9e-60
Identity = 131/184 (71.20%), Postives = 147/184 (79.89%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLGLGLGLTGSMA 60
           MAEL PIS+ +L +R  FSS    +SF+SFN   NCS   TNISRR+GLG    +  S+ 
Sbjct: 1   MAELLPISVSNLTNRIPFSSFRPTISFQSFNTTPNCSINATNISRRLGLGFIALMASSVT 60

Query: 61  ---VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPP 120
              V  ANALDLKLIAP QS +EAESGIR HAEALL+VR LIESEAWKEAQKRLRSSSP 
Sbjct: 61  ATPVELANALDLKLIAPEQSLDEAESGIRSHAEALLQVRSLIESEAWKEAQKRLRSSSPL 120

Query: 121 LKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDI 180
           LKQDL+TIIEYKPPAERP LRKLYS+LFNN+TRMDYAARD+D E VW+CY NI +AISDI
Sbjct: 121 LKQDLYTIIEYKPPAERPALRKLYSVLFNNVTRMDYAARDKDVEGVWKCYNNIAIAISDI 180

Query: 181 LSRI 182
           LSRI
Sbjct: 181 LSRI 184

[Close]

BLAST of MS018654.1 vs. NCBI nr
Match: XP_022962345.1 (psbQ-like protein 3, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 241.9 bits (616), Expect = 4.3e-60
Identity = 134/187 (71.66%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNF-SSSPAVVSFRSFNIAANCSNENTNISRRIGLGLGLGLTGSM 60
           MAEL+PIS+ +L +R +F SSSP + S + F+I A   +   +ISRR+GLGL + L  S 
Sbjct: 1   MAELSPISVSNLSNRIHFSSSSPPINSSQLFSITAKRCSNCRSISRRLGLGL-IALMASS 60

Query: 61  AVGA-----ANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSS 120
           +V +     ANALDLKLIAP QSFEEAESGIRGHAEA+L+VRGLIESE+WKEAQK+LR+S
Sbjct: 61  SVASTPVRIANALDLKLIAPEQSFEEAESGIRGHAEAVLQVRGLIESESWKEAQKQLRTS 120

Query: 121 SPPLKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAI 180
           SP LKQDL+TIIEYKPPAERPPLRKLYSLLFNN+TRMDYAARD+DAERV +CY NIVMAI
Sbjct: 121 SPLLKQDLYTIIEYKPPAERPPLRKLYSLLFNNVTRMDYAARDKDAERVRQCYDNIVMAI 180

Query: 181 SDILSRI 182
           SDILSRI
Sbjct: 181 SDILSRI 186

[Close]

BLAST of MS018654.1 vs. ExPASy Swiss-Prot
Match: Q2V4B2 (PsbQ-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PQL3 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.1e-21
Identity = 53/109 (48.62%), Postives = 72/109 (66.06%), Query Frame = 0


Query: 74  PGQSFEEAESGIRGHAEALLEVRG-LIESEAWKEAQKRLRSSSPPLKQDLHTIIEYKPPA 133
           P ++ E A+ G+R +AE +  ++  +IE + WKE  K LR S+  +KQD + II+ KPP 
Sbjct: 79  PERTVELAQEGLRKNAENIKRIKEIMIEKKLWKEGGKELRRSASNMKQDFYLIIQAKPPK 138

Query: 134 ERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSRI 182
           +RP  R LYS LFN+IT+MDYAARD D  +V E Y NIV  + DI  RI
Sbjct: 139 DRPLFRSLYSSLFNSITKMDYAARDGDETKVLEYYINIVAILDDIFPRI 187

[Close]

BLAST of MS018654.1 vs. ExPASy Swiss-Prot
Match: Q9XFT3 (Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSBQ1 PE=1 SV=3)

HSP 1 Score: 67.8 bits (164), Expect = 1.5e-10
Identity = 34/102 (33.33%), Postives = 61/102 (59.80%), Query Frame = 0


Query: 80  EAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQDLHTIIEYKPPAERPPLRK 139
           EA +  +  A+ ++ V+ LI+ +AW   Q  LRS +  L+ DL+TII  KP  E+  L+ 
Sbjct: 122 EAAARAKESAKDIINVKPLIDRKAWPYVQNDLRSKASYLRYDLNTIISSKPKDEKKSLKD 181

Query: 140 LYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSRI 182
           L + LF+ I  +DYAA+ +   +  + Y   V A++++L+++
Sbjct: 182 LTTKLFDTIDNLDYAAKKKSPSQAEKYYAETVSALNEVLAKL 223

[Close]

BLAST of MS018654.1 vs. ExPASy Swiss-Prot
Match: Q41048 (Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Zea mays OX=4577 GN=PSBQ1 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.6e-07
Identity = 37/136 (27.21%), Postives = 68/136 (50.00%), Query Frame = 0


Query: 54  GLTGSMAVGAANALDLKL--------IAPGQSFEEAESGIRGHAEALLEVRGLIESEAWK 113
           GL G++    A   DL L        + P     EA + ++  A+ ++ ++ LI+ +AW 
Sbjct: 85  GLPGTLNSDQARDFDLPLKERFYLQPLPPA----EAAARVKTSAQDIINLKPLIDKKAWP 144

Query: 114 EAQKRLRSSSPPLKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWE 173
             Q  LR  +  L+ DL T+I  KP  E+  L++L   LF+ I  +D+AA+ +      +
Sbjct: 145 YVQNDLRLRASYLRYDLKTVIASKPKEEKKSLKELTGKLFSTIDDLDHAAKIKSTPEAEK 204

Query: 174 CYGNIVMAISDILSRI 182
            +     A+ D+L+++
Sbjct: 205 YFAATKDALGDVLAKL 216

[Close]

BLAST of MS018654.1 vs. ExPASy Swiss-Prot
Match: Q41806 (Oxygen-evolving enhancer protein 3-2, chloroplastic OS=Zea mays OX=4577 GN=PSBQ2 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.6e-07
Identity = 29/102 (28.43%), Postives = 57/102 (55.88%), Query Frame = 0


Query: 80  EAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQDLHTIIEYKPPAERPPLRK 139
           EA + ++  A+ ++ ++ LI+ +AW   Q  LR  +  L+ DL T+I  KP  E+  L++
Sbjct: 111 EAVARVKTSAQDIINLKPLIDKKAWPYVQNDLRLRASYLRYDLKTVIASKPKEEKKSLKE 170

Query: 140 LYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSRI 182
           L   LF+ I  +D+AA+ +      + +     A+ D+L+++
Sbjct: 171 LTGKLFSTIDDLDHAAKMKSTPEAEKYFAATKDALGDVLAKL 212

[Close]

BLAST of MS018654.1 vs. ExPASy TrEMBL
Match: A0A6J1CJL2 (psbQ-like protein 3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111011901 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.4e-93
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLGLGLGLTGSMA 60
           MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENT+ISRRIGLGLGLGLTGSMA
Sbjct: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTSISRRIGLGLGLGLTGSMA 60

Query: 61  VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQ 120
           VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQ
Sbjct: 61  VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQ 120

Query: 121 DLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSR 180
           DLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSR
Sbjct: 121 DLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSR 180

Query: 181 I 182
           I
Sbjct: 181 I 181

[Close]

BLAST of MS018654.1 vs. ExPASy TrEMBL
Match: A0A6J1JRX6 (psbQ-like protein 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487796 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.8e-64
Identity = 136/185 (73.51%), Postives = 159/185 (85.95%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLG-LGLGLTGSM 60
           MAEL+PIS+ +L +R +FSSSP ++SF+ F+I A   +   +ISRR+GLG + L  + S+
Sbjct: 1   MAELSPISVSNLSNRIHFSSSPPIISFQLFSITAKRCSNCRSISRRLGLGFMALMASSSV 60

Query: 61  A---VGAANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSP 120
           A   V   NALDLKLIAP QSFEEAESGIRGHAEA+L+VRGLIESE+WKEAQK+LRSSSP
Sbjct: 61  ASTPVKITNALDLKLIAPEQSFEEAESGIRGHAEAVLQVRGLIESESWKEAQKQLRSSSP 120

Query: 121 PLKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISD 180
            LKQDL+TIIEYKPPAERPPLRKLYSLLFNN+TRMDYAARD+DAERVW+CY NIVMAISD
Sbjct: 121 LLKQDLYTIIEYKPPAERPPLRKLYSLLFNNVTRMDYAARDKDAERVWQCYDNIVMAISD 180

Query: 181 ILSRI 182
           ILSRI
Sbjct: 181 ILSRI 185

[Close]

BLAST of MS018654.1 vs. ExPASy TrEMBL
Match: A0A6J1HES6 (psbQ-like protein 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462819 PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 2.1e-60
Identity = 134/187 (71.66%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNF-SSSPAVVSFRSFNIAANCSNENTNISRRIGLGLGLGLTGSM 60
           MAEL+PIS+ +L +R +F SSSP + S + F+I A   +   +ISRR+GLGL + L  S 
Sbjct: 1   MAELSPISVSNLSNRIHFSSSSPPINSSQLFSITAKRCSNCRSISRRLGLGL-IALMASS 60

Query: 61  AVGA-----ANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSS 120
           +V +     ANALDLKLIAP QSFEEAESGIRGHAEA+L+VRGLIESE+WKEAQK+LR+S
Sbjct: 61  SVASTPVRIANALDLKLIAPEQSFEEAESGIRGHAEAVLQVRGLIESESWKEAQKQLRTS 120

Query: 121 SPPLKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAI 180
           SP LKQDL+TIIEYKPPAERPPLRKLYSLLFNN+TRMDYAARD+DAERV +CY NIVMAI
Sbjct: 121 SPLLKQDLYTIIEYKPPAERPPLRKLYSLLFNNVTRMDYAARDKDAERVRQCYDNIVMAI 180

Query: 181 SDILSRI 182
           SDILSRI
Sbjct: 181 SDILSRI 186

[Close]

BLAST of MS018654.1 vs. ExPASy TrEMBL
Match: A0A0A0KT23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G315360 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.7e-59
Identity = 131/184 (71.20%), Postives = 147/184 (79.89%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLG-LGLGLTGSM 60
           MA+L PISL +L +R +FS SP  +SF SFN   NCSN  TNISRR+GLG + L  +   
Sbjct: 1   MAKLIPISLSNLTNRIHFSYSPLTISFPSFNTTPNCSNNATNISRRLGLGFIALMASSVT 60

Query: 61  AVGA--ANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPP 120
           A+ A  ANALDLKLIAP QS +EAESGIR HAEALL+VR LIESEAWKEAQKRLRS+S  
Sbjct: 61  AIPAEVANALDLKLIAPEQSLDEAESGIRRHAEALLQVRSLIESEAWKEAQKRLRSTSAL 120

Query: 121 LKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDI 180
           LKQDL+TIIEYKPPAERP LRKLYSLLFNN+TRMDYAARD+D   VWE Y  + MAISDI
Sbjct: 121 LKQDLYTIIEYKPPAERPALRKLYSLLFNNVTRMDYAARDKDVGGVWESYKKMEMAISDI 180

Query: 181 LSRI 182
           LSRI
Sbjct: 181 LSRI 184

[Close]

BLAST of MS018654.1 vs. ExPASy TrEMBL
Match: A0A5A7VLN5 (PsbQ-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold871G00050 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 5.7e-58
Identity = 129/184 (70.11%), Postives = 148/184 (80.43%), Query Frame = 0


Query: 1   MAELNPISLPSLCSRTNFSSSPAVVSFRSFNIAANCSNENTNISRRIGLG-LGLGLTGSM 60
           MA+L PISL +L +R +FSSSP  +SF+SFN   NCS    NISRR+GLG + L  +   
Sbjct: 1   MAKLIPISLSNLTNRIHFSSSPLTISFQSFNTTPNCSKNAKNISRRLGLGFIALMASSVT 60

Query: 61  AVGA--ANALDLKLIAPGQSFEEAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPP 120
           A+ A  ANAL+LKLIAP QS +EAE GIRGHAEALL+VR L+ESEAWKEAQKRLRS+S  
Sbjct: 61  AIPAEVANALNLKLIAPEQSLDEAERGIRGHAEALLQVRSLMESEAWKEAQKRLRSTSAL 120

Query: 121 LKQDLHTIIEYKPPAERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDI 180
           LKQDL+TIIEYKPPAERP LRKLYSLLFNN+TRMDYAARD+DA  V ECY  + MAISDI
Sbjct: 121 LKQDLYTIIEYKPPAERPALRKLYSLLFNNVTRMDYAARDKDAGGVRECYKKMEMAISDI 180

Query: 181 LSRI 182
           LSRI
Sbjct: 181 LSRI 184

[Close]

BLAST of MS018654.1 vs. TAIR 10
Match: AT2G01918.1 (PsbQ-like 3 )

HSP 1 Score: 101.7 bits (252), Expect = 6.5e-22
Identity = 53/109 (48.62%), Postives = 72/109 (66.06%), Query Frame = 0


Query: 74  PGQSFEEAESGIRGHAEALLEVRG-LIESEAWKEAQKRLRSSSPPLKQDLHTIIEYKPPA 133
           P ++ E A+ G+R +AE +  ++  +IE + WKE  K LR S+  +KQD + II+ KPP 
Sbjct: 79  PERTVELAQEGLRKNAENIKRIKEIMIEKKLWKEGGKELRRSASNMKQDFYLIIQAKPPK 138

Query: 134 ERPPLRKLYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSRI 182
           +RP  R LYS LFN+IT+MDYAARD D  +V E Y NIV  + DI  RI
Sbjct: 139 DRPLFRSLYSSLFNSITKMDYAARDGDETKVLEYYINIVAILDDIFPRI 187

[Close]

BLAST of MS018654.1 vs. TAIR 10
Match: AT4G21280.1 (photosystem II subunit QA )

HSP 1 Score: 67.8 bits (164), Expect = 1.0e-11
Identity = 34/102 (33.33%), Postives = 61/102 (59.80%), Query Frame = 0


Query: 80  EAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQDLHTIIEYKPPAERPPLRK 139
           EA +  +  A+ ++ V+ LI+ +AW   Q  LRS +  L+ DL+TII  KP  E+  L+ 
Sbjct: 121 EAAARAKESAKDIINVKPLIDRKAWPYVQNDLRSKASYLRYDLNTIISSKPKDEKKSLKD 180

Query: 140 LYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSRI 182
           L + LF+ I  +DYAA+ +   +  + Y   V A++++L+++
Sbjct: 181 LTTKLFDTIDNLDYAAKKKSPSQAEKYYAETVSALNEVLAKL 222

[Close]

BLAST of MS018654.1 vs. TAIR 10
Match: AT4G21280.2 (photosystem II subunit QA )

HSP 1 Score: 67.8 bits (164), Expect = 1.0e-11
Identity = 34/102 (33.33%), Postives = 61/102 (59.80%), Query Frame = 0


Query: 80  EAESGIRGHAEALLEVRGLIESEAWKEAQKRLRSSSPPLKQDLHTIIEYKPPAERPPLRK 139
           EA +  +  A+ ++ V+ LI+ +AW   Q  LRS +  L+ DL+TII  KP  E+  L+ 
Sbjct: 122 EAAARAKESAKDIINVKPLIDRKAWPYVQNDLRSKASYLRYDLNTIISSKPKDEKKSLKD 181

Query: 140 LYSLLFNNITRMDYAARDRDAERVWECYGNIVMAISDILSRI 182
           L + LF+ I  +DYAA+ +   +  + Y   V A++++L+++
Sbjct: 182 LTTKLFDTIDNLDYAAKKKSPSQAEKYYAETVSALNEVLAKL 223

The following BLAST results are available for this feature:

BLAST of MS018654.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141571.1	5.0e-93	99.45	psbQ-like protein 3, chloroplastic [Momordica charantia]	[more]
XP_022991100.1	3.8e-64	73.51	psbQ-like protein 3, chloroplastic isoform X1 [Cucurbita maxima]	[more]
XP_023551782.1	5.4e-63	71.73	psbQ-like protein 3, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_038877067.1	1.9e-60	71.20	psbQ-like protein 3, chloroplastic [Benincasa hispida]	[more]
XP_022962345.1	4.3e-60	71.66	psbQ-like protein 3, chloroplastic isoform X1 [Cucurbita moschata]	[more]

BLAST of MS018654.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V4B2	9.1e-21	48.62	PsbQ-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PQL3 PE=2 ...	[more]
Q9XFT3	1.5e-10	33.33	Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q41048	2.6e-07	27.21	Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Zea mays OX=4577 GN=PSBQ1...	[more]
Q41806	2.6e-07	28.43	Oxygen-evolving enhancer protein 3-2, chloroplastic OS=Zea mays OX=4577 GN=PSBQ2...	[more]

BLAST of MS018654.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJL2	2.4e-93	99.45	psbQ-like protein 3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101190...	[more]
A0A6J1JRX6	1.8e-64	73.51	psbQ-like protein 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1HES6	2.1e-60	71.66	psbQ-like protein 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A0A0KT23	6.7e-59	71.20	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G315360 PE=3 SV=1	[more]
A0A5A7VLN5	5.7e-58	70.11	PsbQ-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold871G...	[more]

BLAST of MS018654.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G01918.1	6.5e-22	48.62	PsbQ-like 3	[more]
AT4G21280.1	1.0e-11	33.33	photosystem II subunit QA	[more]
AT4G21280.2	1.0e-11	33.33	photosystem II subunit QA	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008797	Oxygen-evolving enhancer protein 3	PFAM	PF05757	PsbQ	coord: 75..180 e-value: 1.7E-17 score: 63.9
IPR008797	Oxygen-evolving enhancer protein 3	PANTHER	PTHR33399	OXYGEN-EVOLVING ENHANCER PROTEIN 3-1, CHLOROPLASTIC	coord: 37..181
IPR023222	PsbQ-like domain superfamily	GENE3D	1.20.120.290		coord: 50..181 e-value: 1.7E-32 score: 114.5
IPR023222	PsbQ-like domain superfamily	SUPERFAMILY	101112	Oxygen-evolving enhancer protein 3,	coord: 74..181
None	No IPR available	PANTHER	PTHR33399:SF6	PSBQ-LIKE PROTEIN 3, CHLOROPLASTIC	coord: 37..181

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS018654	MS018654	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS018654.1-cds	MS018654.1-cds-scaffold313:770950..771399	CDS
MS018654.1-cds	MS018654.1-cds-scaffold313:771482..771574	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS018654.1	MS018654.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015979	photosynthesis
cellular_component	GO:0019898	extrinsic component of membrane
cellular_component	GO:0009523	photosystem II
cellular_component	GO:0009654	photosystem II oxygen evolving complex
molecular_function	GO:0005509	calcium ion binding