MS018539.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS018539.1 vs. NCBI nr
Match: XP_022158990.1 (UPF0481 protein At3g47200-like isoform X2 [Momordica charantia])

HSP 1 Score: 852.8 bits (2202), Expect = 1.2e-243
Identity = 419/423 (99.05%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   EVCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 60
           EVCSSIKKMLQELPPL+EECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH
Sbjct: 30  EVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 89

Query: 61  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELM 120
           KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAE INMDSDEFVKMMLVDGCFIVELM
Sbjct: 90  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 149

Query: 121 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 180
           MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL
Sbjct: 150 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 209

Query: 181 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 240
           THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAI RKK
Sbjct: 210 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAISRKK 269

Query: 241 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 300
           CTFPPTVTELWEAG+VFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY
Sbjct: 270 CTFPPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 329

Query: 301 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 360
           HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD
Sbjct: 330 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 389

Query: 361 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 420
           CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS
Sbjct: 390 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 449

Query: 421 LSK 424
           LSK
Sbjct: 450 LSK 452

BLAST of MS018539.1 vs. NCBI nr
Match: XP_022158992.1 (UPF0481 protein At3g47200-like isoform X3 [Momordica charantia])

HSP 1 Score: 852.8 bits (2202), Expect = 1.2e-243
Identity = 419/423 (99.05%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   EVCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 60
           EVCSSIKKMLQELPPL+EECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH
Sbjct: 8   EVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 67

Query: 61  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELM 120
           KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAE INMDSDEFVKMMLVDGCFIVELM
Sbjct: 68  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 127

Query: 121 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 180
           MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL
Sbjct: 128 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 187

Query: 181 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 240
           THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAI RKK
Sbjct: 188 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAISRKK 247

Query: 241 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 300
           CTFPPTVTELWEAG+VFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY
Sbjct: 248 CTFPPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 307

Query: 301 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 360
           HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD
Sbjct: 308 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 367

Query: 361 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 420
           CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS
Sbjct: 368 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 427

Query: 421 LSK 424
           LSK
Sbjct: 428 LSK 430

BLAST of MS018539.1 vs. NCBI nr
Match: XP_022158989.1 (UPF0481 protein At3g47200-like isoform X1 [Momordica charantia])

HSP 1 Score: 852.8 bits (2202), Expect = 1.2e-243
Identity = 419/423 (99.05%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   EVCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 60
           EVCSSIKKMLQELPPL+EECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH
Sbjct: 53  EVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 112

Query: 61  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELM 120
           KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAE INMDSDEFVKMMLVDGCFIVELM
Sbjct: 113 KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 172

Query: 121 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 180
           MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL
Sbjct: 173 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 232

Query: 181 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 240
           THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAI RKK
Sbjct: 233 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAISRKK 292

Query: 241 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 300
           CTFPPTVTELWEAG+VFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY
Sbjct: 293 CTFPPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 352

Query: 301 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 360
           HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD
Sbjct: 353 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 412

Query: 361 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 420
           CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS
Sbjct: 413 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 472

Query: 421 LSK 424
           LSK
Sbjct: 473 LSK 475

BLAST of MS018539.1 vs. NCBI nr
Match: XP_022132066.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 407.1 bits (1045), Expect = 1.8e-109
Identity = 234/429 (54.55%), Postives = 287/429 (66.90%), Query Frame = 0

Query: 2   VCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 61
           V  S+K ML++L P+SEEC+I+RV +RL   N  AY PQ ISIGPFHHG+++ M MEQ K
Sbjct: 25  VTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLK 84

Query: 62  LRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMM 121
           LRFLD YLRR   GIE    I + WET AR CYAE I+M SD FVKMMLVDG F+VE + 
Sbjct: 85  LRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIR 144

Query: 122 MVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQLT 181
           M  +  + T+   +  LF A+  D+Y DLI+LENQLPFF+L+ L D+ S  +   F+  T
Sbjct: 145 MHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQLPFFILECLLDKCS--SSTPFVLFT 204

Query: 182 HMF---YTRG-PLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIG 241
             F   YT    LI  + L         T K NHLVDFLSFYY    A  + T  +  + 
Sbjct: 205 STFCRWYTGARELISDKLL---------TKKPNHLVDFLSFYY----ALPTVTGKNDKLK 264

Query: 242 RKKCTFPPTVTELWEAGVVFKKAMRAKH-IMDISFKDRVLQIPPLEIGDVFETYVRNLMA 301
             K   PPT TELWEAGV F+KA   K  IMDI FKD VL IP LEI D FETYVRNL+A
Sbjct: 265 YNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLA 324

Query: 302 FEQYH--NDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKD 361
           +E YH  +D +  IQY  FL+ LIS E+DVSLLVKA IITN IGGNN++VS LFNDLCKD
Sbjct: 325 YEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKD 384

Query: 362 VIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQT 421
           + +  D   +  I+  LH++C   W++ MASLRRDYFNTPWAFISF+AA FL+LLT +Q 
Sbjct: 385 INISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQA 438

Query: 422 LFSAMSLSK 424
           ++SA+S  K
Sbjct: 445 IYSAISYHK 438

BLAST of MS018539.1 vs. NCBI nr
Match: XP_022132118.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 378.3 bits (970), Expect = 9.0e-101
Identity = 218/424 (51.42%), Postives = 277/424 (65.33%), Query Frame = 0

Query: 5   SIKKMLQELPPL-SEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLR 64
           SI K+L+E PP+ + E  I+RVP+RL      AY P++I+IGPFHHGR DL+  +Q KL 
Sbjct: 53  SIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLH 112

Query: 65  FLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMMV 124
               YL R    ++  V I R WE  AR  YAE INM SD+FV+MML+D CFIVELM+++
Sbjct: 113 CFRNYLIRIERDVKYVVAIARQWEIKARRLYAEPINMTSDDFVRMMLIDACFIVELMIIL 172

Query: 125 CRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQF---SLEAGLSFLQL 184
                ET   FD L    ++ DLY +L MLENQLPFFVLQ LFD F     +A +SF+QL
Sbjct: 173 NFECFETREGFDLLFAKVILHDLYQELTMLENQLPFFVLQALFDLFPPHKNKANISFIQL 232

Query: 185 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 244
           T  F + G +   R   LP GV  ST +VNHLVD L FYY P+P +  +  +   I  + 
Sbjct: 233 TGKFLSNGLI---RGYNLPCGVS-STEEVNHLVDLLGFYYVPSPDTEEYRQNK-EIKNRI 292

Query: 245 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 304
              PPT+T+L EAGV  KKA+ A+ ++DISFK  VLQIPP EI D FE YVRNLMAFEQY
Sbjct: 293 FLLPPTITKLCEAGVKVKKAIGARSLLDISFKRGVLQIPPFEIHDDFEIYVRNLMAFEQY 352

Query: 305 ----HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVI 364
                +D +Y I Y  FL+GLIS  +DV+LLVK  II N IGG+N+EVS LFN+LCK+  
Sbjct: 353 CVREPDDDRYVIHYIEFLDGLISTAEDVALLVKEGIIINHIGGSNKEVSELFNNLCKNTP 412

Query: 365 VRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLF 421
           +      F   ++ LH+HC   W +  A+LRRDYF++PWA+IS  AA FLILL  LQT+F
Sbjct: 413 IPFQF-YFYATSKNLHDHCRKWWPRSKATLRRDYFHSPWAYISVFAATFLILLALLQTIF 470

BLAST of MS018539.1 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.7e-41
Identity = 122/423 (28.84%), Postives = 203/423 (47.99%), Query Frame = 0

Query: 18  EECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLRFLDRYL---RRTNF 77
           E C I RVP   +  N +AY P+++SIGP+H+G + L  ++QHK R L  +L   ++ + 
Sbjct: 44  ESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDV 103

Query: 78  GIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMMVCRIGSETETRF 137
              V V  V   E   R  Y+E +    D  + MM++DGCFI   +M+   +    E   
Sbjct: 104 EENVLVKAVVDLEDKIRKSYSEELKTGHD-LMFMMVLDGCFI---LMVFLIMSGNIELSE 163

Query: 138 DPLLFCA-MMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQLTHMFYTRGPLIKP 197
           DP+     +++ +  DL++LENQ+PFFVLQ L+    +       ++   F+ + P+ K 
Sbjct: 164 DPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFHFF-KNPIDKE 223

Query: 198 RTLELPHGVMISTHKVNHLVDFLSFYYAP--------APASVSFTSHSLAIGRKKCTFPP 257
            +    H      +K  HL+D +   + P        +   V    H    G        
Sbjct: 224 GSYWEKH----RNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLHEGKSGNVPSVDSK 283

Query: 258 TVTELWEA------GVVFK-KAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFE 317
            V  +  A      G+ F+ +  +   I+++  K   LQIP L       ++  N +AFE
Sbjct: 284 AVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFE 343

Query: 318 QYHNDGKYAI-QYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIV 377
           Q++ D    I  Y +F+  L++ E+DV+ L    +I     G+N EVS  F  + KDV+ 
Sbjct: 344 QFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISKDVVF 403

Query: 378 RGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFS 421
             D +  N++ + ++E+    +N   A  R  +F +PW F+S  A  F+ILLT LQ+  +
Sbjct: 404 EVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVILLTMLQSTVA 457

BLAST of MS018539.1 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 9.2e-16
Identity = 108/487 (22.18%), Postives = 199/487 (40.86%), Query Frame = 0

Query: 21  NIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLRFLDRYLRRTN-FGIEVT 80
           +I  VP+ L+  +  +Y P  +SIGP+H  + +L  ME++KL    +   + N F     
Sbjct: 44  SIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHDL 103

Query: 81  VGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMMVCRIGSETETRFDPLLF 140
           V  ++S E   R CY + I  + +  + +M VD  F++E + +       +  + + L+ 
Sbjct: 104 VEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFLIEFLKIY------SFRKVETLIN 163

Query: 141 CAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEA-----GLSFLQLTHMFYTRGPLIKPR 200
                ++  D++M+ENQ+P FVL+    +F LE+      L    LT +     PL+   
Sbjct: 164 RVGHNEILRDIMMIENQIPLFVLRKTL-EFQLESTESADDLLLSVLTGLCKDLSPLV--I 223

Query: 201 TLELPHGVMISTHKVNHLVDFL-------------------------------------- 260
             +    +     + NH++DFL                                      
Sbjct: 224 KFDDDQILKAQFQECNHILDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIK 283

Query: 261 ---SFYYAPAPASV--------------------------------SFTSHSLAIGRKKC 320
                 +A  PA +                                + T+   ++     
Sbjct: 284 HQFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDI 343

Query: 321 TFP--------PTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQ--IPPLEIGDVFETYV 380
             P        P+V++L +AGV FK      +I  ++F     Q  +P + +    ET +
Sbjct: 344 EKPPLVEELTIPSVSDLHKAGVRFKPTAHG-NISTVTFDSNSGQFYLPVINLDINTETVL 403

Query: 381 RNLMAFEQYHNDGKYAI-QYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFND 418
           RNL+A+E  +  G     +Y   +NG+I  E+DV LL +  ++ + +  ++QE + ++N 
Sbjct: 404 RNLVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRL-KSDQEAAEMWNG 463

BLAST of MS018539.1 vs. ExPASy TrEMBL
Match: A0A6J1DXD6 (UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.0e-244
Identity = 419/423 (99.05%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   EVCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 60
           EVCSSIKKMLQELPPL+EECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH
Sbjct: 30  EVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 89

Query: 61  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELM 120
           KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAE INMDSDEFVKMMLVDGCFIVELM
Sbjct: 90  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 149

Query: 121 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 180
           MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL
Sbjct: 150 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 209

Query: 181 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 240
           THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAI RKK
Sbjct: 210 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAISRKK 269

Query: 241 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 300
           CTFPPTVTELWEAG+VFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY
Sbjct: 270 CTFPPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 329

Query: 301 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 360
           HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD
Sbjct: 330 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 389

Query: 361 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 420
           CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS
Sbjct: 390 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 449

Query: 421 LSK 424
           LSK
Sbjct: 450 LSK 452

BLAST of MS018539.1 vs. ExPASy TrEMBL
Match: A0A6J1E120 (UPF0481 protein At3g47200-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.0e-244
Identity = 419/423 (99.05%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   EVCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 60
           EVCSSIKKMLQELPPL+EECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH
Sbjct: 53  EVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 112

Query: 61  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELM 120
           KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAE INMDSDEFVKMMLVDGCFIVELM
Sbjct: 113 KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 172

Query: 121 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 180
           MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL
Sbjct: 173 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 232

Query: 181 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 240
           THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAI RKK
Sbjct: 233 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAISRKK 292

Query: 241 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 300
           CTFPPTVTELWEAG+VFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY
Sbjct: 293 CTFPPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 352

Query: 301 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 360
           HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD
Sbjct: 353 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 412

Query: 361 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 420
           CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS
Sbjct: 413 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 472

Query: 421 LSK 424
           LSK
Sbjct: 473 LSK 475

BLAST of MS018539.1 vs. ExPASy TrEMBL
Match: A0A6J1DYL4 (UPF0481 protein At3g47200-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.0e-244
Identity = 419/423 (99.05%), Postives = 421/423 (99.53%), Query Frame = 0

Query: 1   EVCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 60
           EVCSSIKKMLQELPPL+EECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH
Sbjct: 8   EVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQH 67

Query: 61  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELM 120
           KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAE INMDSDEFVKMMLVDGCFIVELM
Sbjct: 68  KLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 127

Query: 121 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 180
           MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL
Sbjct: 128 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQL 187

Query: 181 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 240
           THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAI RKK
Sbjct: 188 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAISRKK 247

Query: 241 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 300
           CTFPPTVTELWEAG+VFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY
Sbjct: 248 CTFPPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 307

Query: 301 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 360
           HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD
Sbjct: 308 HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGD 367

Query: 361 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 420
           CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS
Sbjct: 368 CNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMS 427

Query: 421 LSK 424
           LSK
Sbjct: 428 LSK 430

BLAST of MS018539.1 vs. ExPASy TrEMBL
Match: A0A6J1BR71 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005028 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 8.7e-110
Identity = 234/429 (54.55%), Postives = 287/429 (66.90%), Query Frame = 0

Query: 2   VCSSIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 61
           V  S+K ML++L P+SEEC+I+RV +RL   N  AY PQ ISIGPFHHG+++ M MEQ K
Sbjct: 25  VTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLK 84

Query: 62  LRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMM 121
           LRFLD YLRR   GIE    I + WET AR CYAE I+M SD FVKMMLVDG F+VE + 
Sbjct: 85  LRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIR 144

Query: 122 MVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQLT 181
           M  +  + T+   +  LF A+  D+Y DLI+LENQLPFF+L+ L D+ S  +   F+  T
Sbjct: 145 MHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQLPFFILECLLDKCS--SSTPFVLFT 204

Query: 182 HMF---YTRG-PLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIG 241
             F   YT    LI  + L         T K NHLVDFLSFYY    A  + T  +  + 
Sbjct: 205 STFCRWYTGARELISDKLL---------TKKPNHLVDFLSFYY----ALPTVTGKNDKLK 264

Query: 242 RKKCTFPPTVTELWEAGVVFKKAMRAKH-IMDISFKDRVLQIPPLEIGDVFETYVRNLMA 301
             K   PPT TELWEAGV F+KA   K  IMDI FKD VL IP LEI D FETYVRNL+A
Sbjct: 265 YNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLA 324

Query: 302 FEQYH--NDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKD 361
           +E YH  +D +  IQY  FL+ LIS E+DVSLLVKA IITN IGGNN++VS LFNDLCKD
Sbjct: 325 YEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKD 384

Query: 362 VIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQT 421
           + +  D   +  I+  LH++C   W++ MASLRRDYFNTPWAFISF+AA FL+LLT +Q 
Sbjct: 385 INISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQA 438

Query: 422 LFSAMSLSK 424
           ++SA+S  K
Sbjct: 445 IYSAISYHK 438

BLAST of MS018539.1 vs. ExPASy TrEMBL
Match: A0A6J1BVD4 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 4.3e-101
Identity = 218/424 (51.42%), Postives = 277/424 (65.33%), Query Frame = 0

Query: 5   SIKKMLQELPPL-SEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLR 64
           SI K+L+E PP+ + E  I+RVP+RL      AY P++I+IGPFHHGR DL+  +Q KL 
Sbjct: 53  SIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLH 112

Query: 65  FLDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMMV 124
               YL R    ++  V I R WE  AR  YAE INM SD+FV+MML+D CFIVELM+++
Sbjct: 113 CFRNYLIRIERDVKYVVAIARQWEIKARRLYAEPINMTSDDFVRMMLIDACFIVELMIIL 172

Query: 125 CRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQF---SLEAGLSFLQL 184
                ET   FD L    ++ DLY +L MLENQLPFFVLQ LFD F     +A +SF+QL
Sbjct: 173 NFECFETREGFDLLFAKVILHDLYQELTMLENQLPFFVLQALFDLFPPHKNKANISFIQL 232

Query: 185 THMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKK 244
           T  F + G +   R   LP GV  ST +VNHLVD L FYY P+P +  +  +   I  + 
Sbjct: 233 TGKFLSNGLI---RGYNLPCGVS-STEEVNHLVDLLGFYYVPSPDTEEYRQNK-EIKNRI 292

Query: 245 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 304
              PPT+T+L EAGV  KKA+ A+ ++DISFK  VLQIPP EI D FE YVRNLMAFEQY
Sbjct: 293 FLLPPTITKLCEAGVKVKKAIGARSLLDISFKRGVLQIPPFEIHDDFEIYVRNLMAFEQY 352

Query: 305 ----HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVI 364
                +D +Y I Y  FL+GLIS  +DV+LLVK  II N IGG+N+EVS LFN+LCK+  
Sbjct: 353 CVREPDDDRYVIHYIEFLDGLISTAEDVALLVKEGIIINHIGGSNKEVSELFNNLCKNTP 412

Query: 365 VRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLF 421
           +      F   ++ LH+HC   W +  A+LRRDYF++PWA+IS  AA FLILL  LQT+F
Sbjct: 413 IPFQF-YFYATSKNLHDHCRKWWPRSKATLRRDYFHSPWAYISVFAATFLILLALLQTIF 470

BLAST of MS018539.1 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-66
Identity = 158/425 (37.18%), Postives = 236/425 (55.53%), Query Frame = 0

Query: 5   SIKKMLQELPPLSEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLRF 64
           SIK  L  L  LS +C I++VP +L + N  AY P+++S GP H G+++L  ME  K R+
Sbjct: 278 SIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQKYRY 337

Query: 65  LDRYLRRTNFGIEVTVGIVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMM-- 124
           L  ++ RTN  +E  V + R+WE  AR+CYAE + + SDEFV+M++VDG F+VEL++   
Sbjct: 338 LLSFIPRTNSSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLVELLLRSH 397

Query: 125 VCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLF---DQFSLEAGLSFLQ 184
             R+  E +  F   +   M+TD+  D+I++ENQLPFFV++ +F     +  +   S +Q
Sbjct: 398 YPRLRGENDRIFGNSM---MITDVCRDMILIENQLPFFVVKEIFLLLLNYYQQGTPSIIQ 457

Query: 185 LT--HMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPA-PASVSFTSHSLAI 244
           L   H  Y    +   + +  P           H VD L   Y P  P  + +T+     
Sbjct: 458 LAQRHFSYFLSRIDDEKFITEP----------EHFVDLLRSCYLPQFPIKLEYTT----- 517

Query: 245 GRKKCTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMA 304
              K    P  TEL  AGV FK A  +  ++DISF D VL+IP + + D+ E+  +N++ 
Sbjct: 518 --VKVDNAPEATELHTAGVRFKPAETSSCLLDISFADGVLKIPTIVVDDLTESLYKNIIG 577

Query: 305 FEQYHNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVI 364
           FEQ     K  + Y + L   I    D  LL+ + II N + GN+ +VS LFN + K+VI
Sbjct: 578 FEQCRCSNKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYL-GNSVDVSNLFNSISKEVI 637

Query: 365 VRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLF 422
                  F+ ++E L  +C   WN+  A LRRDYF+ PWA  S  AA  L+LLTF+Q++ 
Sbjct: 638 YDRRF-YFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLTFIQSVC 680

BLAST of MS018539.1 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 207.6 bits (527), Expect = 2.0e-53
Identity = 140/424 (33.02%), Postives = 224/424 (52.83%), Query Frame = 0

Query: 22  IHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLRFLDRYLRRTNFGIEVTVG 81
           I+RVP  L + + ++Y PQ +S+GP+HHG++ L PME+HK R L++ L+R    IE+   
Sbjct: 115 IYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRPMERHKWRALNKVLKRLKQRIEMYTN 174

Query: 82  IVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMMVCRIGSET-ETRFDPLL-F 141
            +R  E  AR CY   I++  +EF +M+++DGCF++EL        +E    R DP+   
Sbjct: 175 AMRELEEKARACYEGPISLSRNEFTEMLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAM 234

Query: 142 CAMMTDLYCDLIMLENQLPFFVLQGLFD-------QFSLEAGLSFLQLTHMFYTRGPLIK 201
             +M  +  D+IMLENQLP FVL  L +       Q  + A ++      +  T   L K
Sbjct: 235 RGLMHSIQRDMIMLENQLPLFVLDRLLELQLGTQNQTGIVAHVAVKFFDPLMPTGEALTK 294

Query: 202 PRTLELPHGVMISTHKVN-----HLVDFLS---FYYAPAPASVS----FTSHSLAIGRKK 261
           P   +L + +  S   +      H +D         +P P + S     T ++  + +++
Sbjct: 295 PDQSKLMNWLEKSLDTLGDKGELHCLDVFRRSLLQSSPTPNTRSLLKRLTRNTRVVDKRQ 354

Query: 262 CTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQY 321
                 VTEL EAGV F+K  +     DI FK+  L+IP L I D  ++   NL+AFEQ 
Sbjct: 355 QQLVHCVTELREAGVKFRK-RKTDRFWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFEQC 414

Query: 322 H-NDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRG 381
           H     +   Y +F++ LI+  +DVS L    II + + G++ EV+ LFN LC++V+   
Sbjct: 415 HIESSNHITSYIIFMDNLINSSEDVSYLHYCGIIEHWL-GSDSEVADLFNRLCQEVVFDP 474

Query: 382 DCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAM 424
             +  + ++  ++ +   +WN   A+L   YFN PWA+ SF AA  L+LLT  Q+ ++  
Sbjct: 475 KDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYAVY 534

BLAST of MS018539.1 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 205.3 bits (521), Expect = 9.7e-53
Identity = 138/426 (32.39%), Postives = 228/426 (53.52%), Query Frame = 0

Query: 22  IHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLRFLDRYLRRTNFGIEVTVG 81
           I+RVP  L + + ++Y PQ +S+GP+HHG++ L  M++HK R ++R L+RTN GI++ + 
Sbjct: 105 IYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYID 164

Query: 82  IVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMMVCRIGSET-ETRFDPLL-F 141
            +R  E  AR CY   +++ S+EF++M+++DGCF++EL        +E    R DP+   
Sbjct: 165 AMRELEEKARACYEGPLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFAM 224

Query: 142 CAMMTDLYCDLIMLENQLPFFVLQGLFD-------QFSLEAGLSFLQLTHMFYTRGPLIK 201
              M  +  D++MLENQLP FVL  L +       Q  L A L+      +  T  PL K
Sbjct: 225 RGSMHSIQRDMVMLENQLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEPLTK 284

Query: 202 PRTLELPHGVM-------ISTHKVNHLVD-----FLSFYYAPAP--ASVSFTSHSLAIGR 261
               +L + +         +     H +D      L     P P      ++ ++    +
Sbjct: 285 SGQSKLENSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRVADK 344

Query: 262 KKCTFPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFE 321
           ++      VTEL EAG+ F++  +     D+ FK+  L+IP L I D  ++   NL+AFE
Sbjct: 345 RRQQLIHCVTELKEAGIKFRR-RKTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFE 404

Query: 322 QYHNDGKYAI-QYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIV 381
           Q H D    I  Y +F++ LI   +DVS L    II + + G++ EV+ LFN LC++V+ 
Sbjct: 405 QCHIDSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWL-GSDSEVADLFNRLCQEVVF 464

Query: 382 RGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFS 424
             + +  + ++  ++ +   +WN   A+L+  YFN PWA +SF AA  L++LTF Q+ ++
Sbjct: 465 DTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQSFYA 524

BLAST of MS018539.1 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-52
Identity = 142/406 (34.98%), Postives = 215/406 (52.96%), Query Frame = 0

Query: 22  IHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLRFLDRYLRRTNFGIEVTVG 81
           I+RVP  L + + ++YMPQI+SIGP+HHG + LMPME+HK R ++  + R    IE+ + 
Sbjct: 106 IYRVPPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYID 165

Query: 82  IVRSWETTARNCYAESINMDSDEFVKMMLVDGCFIVELMMMVCRIGSET-ETRFDPLL-F 141
            ++  E  AR CY   INM+ +EF++M+++DG FI+E+         E      DP+   
Sbjct: 166 AMKELEEKARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSEGFQEIGYAPNDPVFGM 225

Query: 142 CAMMTDLYCDLIMLENQLPFFVLQGLFD-QFSLEAGLSFLQLTHMFYTRGPLIKPRTLEL 201
             +M  +  D++MLENQLP+ VL+GL   Q         +QL   F+   PL+  R +  
Sbjct: 226 RGLMQSIRRDMVMLENQLPWSVLKGLLQLQRPDVLDKVNVQLFQPFFQ--PLLPTREVLT 285

Query: 202 PHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIGRKKCTFPPTVTELWEAGVVFK 261
             G +       H +D L        +S +       + ++       VTEL  AGV F 
Sbjct: 286 EEGGL-------HCLDVLR--RGLLQSSGTSDEDMSMVNKQPQQLIHCVTELRNAGVEFM 345

Query: 262 KAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQYH-NDGKYAIQYFLFLNGL 321
           +     H  DI FK+  L+IP L I D  ++   NL+AFEQ H    K    Y +F++ L
Sbjct: 346 R-KETGHFWDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFEQCHIKSSKKITSYIIFMDNL 405

Query: 322 ISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGA 381
           I+  +DVS L    II N + G++ EVS LFN L K+VI   +    + +   ++ +   
Sbjct: 406 INSSEDVSYLHHYGIIENWL-GSDSEVSDLFNGLGKEVIFDPNDGYLSALTGEVNIYYRR 465

Query: 382 RWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMSLSK 424
           +WN   A+LR  YFN PWA+ SF+AA  L++ TF Q+ F+  +  K
Sbjct: 466 KWNYLKATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFAVFAYFK 498

BLAST of MS018539.1 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 200.7 bits (509), Expect = 2.4e-51
Identity = 146/422 (34.60%), Postives = 219/422 (51.90%), Query Frame = 0

Query: 22  IHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHKLRFLDRYLRRTNFGIEVTVG 81
           I+RVP  L + + ++Y+PQ +SIGP+HHG+  L PME+HK R ++  + RT   IE+ + 
Sbjct: 92  IYRVPFYLQENDKKSYLPQTVSIGPYHHGKVHLRPMERHKWRAVNMIMARTKHNIEMYID 151

Query: 82  IVRSWETTARNCYAESINM-DSDEFVKMMLVDGCFIVELMMMVC----RIGSETETRFDP 141
            ++  E  AR CY   I+M +S+EF +M+++DGCF++EL         +IG     R DP
Sbjct: 152 AMKELEEEARACYQGPIDMKNSNEFTEMLVLDGCFVLELFKGTIQGFQKIG---YARNDP 211

Query: 142 LLF-CAMMTDLYCDLIMLENQLPFFVLQ---GLFDQFSLEAGLSFLQLTHMFYTRGPLIK 201
           +     +M  +  D+IMLENQLP FVL    GL      + G+        F T  P   
Sbjct: 212 VFAKRGLMHSIQRDMIMLENQLPLFVLDRLLGLQTGTPNQTGIVAEVAVRFFKTLMP--T 271

Query: 202 PRTLELPHGVMISTHKVNHLVD-----FLSFYYAPAPASVSFTS-----HSLAIGRKKCT 261
              L      + S  K + L D      L  ++     S   T+       +++  K+  
Sbjct: 272 SEVLTKSERSLDSQEKSDELGDNGGLHCLDVFHRSLIQSSETTNQGTPYEDMSMVEKQQQ 331

Query: 262 FPPTVTELWEAGVVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRNLMAFEQYHN 321
               VTEL  AGV F +      + DI FK+  L+IP L I D  ++   NL+AFEQ H 
Sbjct: 332 LIHCVTELRGAGVNFMR-KETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHT 391

Query: 322 DGKYAI-QYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKDVIVRGDC 381
                I  Y +F++ LI+  QDVS L    II + + G++ EV+ LFN LCK+VI     
Sbjct: 392 QSSNNITSYIIFMDNLINSSQDVSYLHHDGIIEHWL-GSDSEVADLFNRLCKEVIFDPKD 451

Query: 382 NCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQTLFSAMSL 424
              + ++  ++ +   +WN   A+LR+ YFN PWA+ SF AA  L+ LTF Q+ F+  + 
Sbjct: 452 GYLSQLSREVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVYAY 506