MS016853.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS016853.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	TLC domain-containing protein
Location	scaffold9_1: 493985 .. 495776 (+)
Sequence length	696
RNA-Seq Expression	MS016853.1
Synteny	MS016853.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTTTCCGGGAGTCGAGATCCCGACGTAGTAAATGTCGTCGCTTTGGGGGTGATATCGTGGACAACGGCTTTCGTTTTGGTCAGAAAACTCCTCCGGAACCGATCTTTTGAGCTCTGCAACCGTGTGGTCTCCACTCTCCATGCTGCCTTGGCCGTCACGTTGGCTTCCCTCTCTGTCCAAGATTGGCGATGCCCCATTTGTCCTCTCGCTTCCCAATCCTCACCATTTCAGGTAAATTCAACTTAAATCTCAGGATCCATTGGATAACTATTTTATTTTTAGTTTTTTTTTTCCTTTTGTAACATAAGAGCAAAAGTAATGTTTCTGTGTTTAATTTTAAAAAATAGAAAATTAAAATTGATACGGTTATCAAAAGAAACTTACATTGTTAAATGGGTGGATGTGTTAGCCAGATGCAAATATTGGGGGTGAGCTGTTCTTATTTGATATATGATATGGTGTGTTGCCACTTTGACAAAAGAGTGAGTGTGGACAATACCATCCATCATTTGATTAGCATCGTCGGTATTGCAGCAGGCTTAGCTTATCAAAAGGTAAATTCATTTTACTCCAATAAAATTTCTAGTACTATTGAAATAGTTATTCGAGTATCCATGCTCTTGTAGTACCATAAATTAACCTTGTGGTTTACTGTTGATTATATTTATAATTTGTTAGCGATATTTTTGGAATATTTAATTTTAATTATGATAAATTAATAAATATGTATGTAGTGTGGGTCAGAGATGGTAGCAGCATTGTTGATAACAGAGATCTCAAGCCCCTTTCTCCATTTGAGGGAAATTCTCAAAGAAATTGGTTACAGAAATACTGAACTTAATTTTGCAGCTGATGTAAGTCACCCTTTTATTTTTCCTAATACTATTTTGGTCCAACACTTTCTGTTTTAAGTCTATATATTTTCAAAACGTCTATTTTGACCCTTGAAATGTTCAACTTGGTCAACGTATTTGCGGCTGTTTTGGTTCTTTCATTTTATTTTTTTATCTAATTTTTTTAAATTACAATTTCAATAGACACCACATCCAATAAATTTTTTGAAATGTAATATAACTTTGTATTAAAGGGTTTTCGTTGTTTTTGGAATGTGTGAAAATTTTAAACATGGATCAAAATAATCAATCGCATTTTAAGATTACAAGGATCAAAACGGACGTATTTAAAGTACAATATCAAAGAGTTAATGTTACATTGACATTGTTTATGAACAAATTGAAAAGGATTTGATTTTTTAGCAAAGTAAAATTAGTTTGAAAAAATAGAAGGCTCCAAAAGCCCATTAATCATAATTGATATGAGATTATGACATGAATTTGAAAGTTAAAAGCTGATTGTATTGACAGATTGCGTTTGCAGTGATCTTCTCATTCGCAAGAATGGTTGGAGGGCCTTATCTCACCTACCTTACTTTATCCTCTAACGTCCCTCTTCTTATTAAGGTAATCTTTTCAAACTTCGAATCTCATGGAATGTCGATATGGTTACCAAATACATTTCTTTTTCTTTTTTCTTTTTCAATAAAAAAGAAAAGAATAAGAAATAGTTGCTTTTCTTTATTGAAAAAAGGAGACTAAACCAGTAATAAAAATGTTACCCAATGCATCCTAATTTTTCCAACGATCTACTTTTGAGTTTTGACCAATTAGTTTATATAATTGTTCAGGCAATGGCATTGGGATTGCAGCTTGTGAGTGCCTACTGGTTTTACAAGATCGTGAGAATGGTCAGATTCAAACTCAACAACAGATCTACATTGAAGAATAAT

mRNA sequence

ATGGCGGTTTCCGGGAGTCGAGATCCCGACGTAGTAAATGTCGTCGCTTTGGGGGTGATATCGTGGACAACGGCTTTCGTTTTGGTCAGAAAACTCCTCCGGAACCGATCTTTTGAGCTCTGCAACCGTGTGGTCTCCACTCTCCATGCTGCCTTGGCCGTCACGTTGGCTTCCCTCTCTGTCCAAGATTGGCGATGCCCCATTTGTCCTCTCGCTTCCCAATCCTCACCATTTCAGATGCAAATATTGGGGGTGAGCTGTTCTTATTTGATATATGATATGGTGTGTTGCCACTTTGACAAAAGAGTGAGTGTGGACAATACCATCCATCATTTGATTAGCATCGTCGGTATTGCAGCAGGCTTAGCTTATCAAAAGTGTGGGTCAGAGATGGTAGCAGCATTGTTGATAACAGAGATCTCAAGCCCCTTTCTCCATTTGAGGGAAATTCTCAAAGAAATTGGTTACAGAAATACTGAACTTAATTTTGCAGCTGATATTGCGTTTGCAGTGATCTTCTCATTCGCAAGAATGGTTGGAGGGCCTTATCTCACCTACCTTACTTTATCCTCTAACGTCCCTCTTCTTATTAAGGCAATGGCATTGGGATTGCAGCTTGTGAGTGCCTACTGGTTTTACAAGATCGTGAGAATGGTCAGATTCAAACTCAACAACAGATCTACATTGAAGAATAAT

Coding sequence (CDS)

ATGGCGGTTTCCGGGAGTCGAGATCCCGACGTAGTAAATGTCGTCGCTTTGGGGGTGATATCGTGGACAACGGCTTTCGTTTTGGTCAGAAAACTCCTCCGGAACCGATCTTTTGAGCTCTGCAACCGTGTGGTCTCCACTCTCCATGCTGCCTTGGCCGTCACGTTGGCTTCCCTCTCTGTCCAAGATTGGCGATGCCCCATTTGTCCTCTCGCTTCCCAATCCTCACCATTTCAGATGCAAATATTGGGGGTGAGCTGTTCTTATTTGATATATGATATGGTGTGTTGCCACTTTGACAAAAGAGTGAGTGTGGACAATACCATCCATCATTTGATTAGCATCGTCGGTATTGCAGCAGGCTTAGCTTATCAAAAGTGTGGGTCAGAGATGGTAGCAGCATTGTTGATAACAGAGATCTCAAGCCCCTTTCTCCATTTGAGGGAAATTCTCAAAGAAATTGGTTACAGAAATACTGAACTTAATTTTGCAGCTGATATTGCGTTTGCAGTGATCTTCTCATTCGCAAGAATGGTTGGAGGGCCTTATCTCACCTACCTTACTTTATCCTCTAACGTCCCTCTTCTTATTAAGGCAATGGCATTGGGATTGCAGCTTGTGAGTGCCTACTGGTTTTACAAGATCGTGAGAATGGTCAGATTCAAACTCAACAACAGATCTACATTGAAGAATAAT

Protein sequence

MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLSVQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN

Homology

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BLAST of MS016853.1 vs. NCBI nr
Match: XP_022144270.1 (transmembrane protein 136-like isoform X1 [Momordica charantia])

HSP 1 Score: 447.6 bits (1150), Expect = 6.6e-122
Identity = 231/232 (99.57%), Postives = 231/232 (99.57%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60
           MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS
Sbjct: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60

Query: 61  VQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120
           VQD RCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA
Sbjct: 61  VQDLRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120

Query: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180
           GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG
Sbjct: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180

Query: 181 GPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN
Sbjct: 181 GPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 232

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BLAST of MS016853.1 vs. NCBI nr
Match: XP_038886579.1 (TLC domain-containing protein 5-like [Benincasa hispida])

HSP 1 Score: 390.6 bits (1002), Expect = 9.6e-105
Identity = 192/232 (82.76%), Postives = 211/232 (90.95%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60
           MAVS SRDP V+N+V  GVISWT  F+ +RKLL NRSFE CNR++ST+HA LAVTLAS+S
Sbjct: 1   MAVSDSRDPHVINIVVFGVISWTLGFIFLRKLLPNRSFEFCNRLISTIHACLAVTLASIS 60

Query: 61  VQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120
           V++WRCPICPLASQSSPFQMQ L VSCSYLIYDMVCCHFDK+VS+DNTIHHL+SIVGIAA
Sbjct: 61  VRNWRCPICPLASQSSPFQMQTLAVSCSYLIYDMVCCHFDKKVSLDNTIHHLVSIVGIAA 120

Query: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180
           G AYQKCGSEMVAAL ITEISSPFLHLREILKEIGY+ T+LN AADI FAVIFS ARMVG
Sbjct: 121 GFAYQKCGSEMVAALWITEISSPFLHLREILKEIGYKGTDLNLAADIGFAVIFSSARMVG 180

Query: 181 GPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GPYLTY+TL++ VP LIKAMALGLQLVSAYWFYKIVRMVRFKLNNR TLKN+
Sbjct: 181 GPYLTYMTLTATVPFLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRLTLKNS 232

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BLAST of MS016853.1 vs. NCBI nr
Match: KAG7029404.1 (Transmembrane protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389.4 bits (999), Expect = 2.1e-104
Identity = 196/233 (84.12%), Postives = 211/233 (90.56%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60
           MAVS  RDPDV+NVV LGVI+WT AF+  RKLL NRSFE CNR+VSTLHA LAVTLAS+S
Sbjct: 1   MAVSTGRDPDVINVVVLGVIAWTLAFISFRKLLPNRSFEFCNRLVSTLHACLAVTLASIS 60

Query: 61  VQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120
           VQ+WRCPICPLASQ+SPFQMQ L V+CSYLIYDMVCCHFDK+V+VDN IHHL+ IVGIAA
Sbjct: 61  VQNWRCPICPLASQASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVCIVGIAA 120

Query: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180
           G AYQKCGSEMVAAL ITEISSPFLHLREILKEIGYR T+LN AADI FAVIFSF RMVG
Sbjct: 121 GFAYQKCGSEMVAALWITEISSPFLHLREILKEIGYRGTDLNLAADIGFAVIFSFGRMVG 180

Query: 181 GPYLTYLTLS-SNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GPYLTY+TLS + VPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRST KN+
Sbjct: 181 GPYLTYMTLSATGVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTSKNS 233

[Close]

BLAST of MS016853.1 vs. NCBI nr
Match: KAG6598466.1 (TLC domain-containing protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 389.0 bits (998), Expect = 2.8e-104
Identity = 195/233 (83.69%), Postives = 211/233 (90.56%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60
           MAVS  RDPDV+NVV LGVI+WT AF+  RKLL NRSFE CNR+VSTLHA LAVTLAS+S
Sbjct: 1   MAVSTGRDPDVINVVVLGVIAWTLAFISFRKLLPNRSFEFCNRLVSTLHACLAVTLASIS 60

Query: 61  VQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120
           VQ+WRCPICPLASQ+SPFQMQ L V+CSYLIYDMVCCHFDK+V+VDN IHHL+ IVGIAA
Sbjct: 61  VQNWRCPICPLASQASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVCIVGIAA 120

Query: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180
           G AYQKCGSEMVAAL ITEISSPFLHLREILKEIGYR T+LN AADI FAVIFSF RMVG
Sbjct: 121 GFAYQKCGSEMVAALWITEISSPFLHLREILKEIGYRGTDLNLAADIGFAVIFSFGRMVG 180

Query: 181 GPYLTYLTLS-SNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GPYLTY+TLS + VPLLIKAMALGLQLVSAYWFYKIVRM+RFKLNNRST KN+
Sbjct: 181 GPYLTYMTLSATGVPLLIKAMALGLQLVSAYWFYKIVRMIRFKLNNRSTSKNS 233

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BLAST of MS016853.1 vs. NCBI nr
Match: XP_008444752.1 (PREDICTED: transmembrane protein 136 [Cucumis melo] >KAA0065207.1 transmembrane protein 136 [Cucumis melo var. makuwa])

HSP 1 Score: 388.7 bits (997), Expect = 3.6e-104
Identity = 193/233 (82.83%), Postives = 213/233 (91.42%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLL-RNRSFELCNRVVSTLHAALAVTLASL 60
           M VS +RDPD++NVV  GVISWT  FVL+RK++  NRSFE CNR++ST+HA LAVTLAS+
Sbjct: 1   MEVSETRDPDIINVVVYGVISWTLGFVLLRKVVFPNRSFEFCNRLISTIHAFLAVTLASI 60

Query: 61  SVQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIA 120
           SVQ+WRCPICPLAS+SS FQMQ LGVSCSYLIYDMVCCHFDK+VS+DNTIHHL+SIVGIA
Sbjct: 61  SVQNWRCPICPLASKSSSFQMQTLGVSCSYLIYDMVCCHFDKKVSLDNTIHHLVSIVGIA 120

Query: 121 AGLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMV 180
           AG AYQKCGSEMVAAL ITEISSPFLHLREILKE GYR T+LN AADI FAVIFSFARMV
Sbjct: 121 AGFAYQKCGSEMVAALWITEISSPFLHLREILKEFGYRGTDLNLAADIGFAVIFSFARMV 180

Query: 181 GGPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GGPYLTY+TLS+NVP LIKAMALGLQLVSAYWFYKIVRM+RFKL NRSTLKN+
Sbjct: 181 GGPYLTYMTLSANVPFLIKAMALGLQLVSAYWFYKIVRMIRFKLKNRSTLKNS 233

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BLAST of MS016853.1 vs. ExPASy Swiss-Prot
Match: Q0VD42 (TLC domain-containing protein 5 OS=Bos taurus OX=9913 GN=TLCD5 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.1e-13
Identity = 54/198 (27.27%), Postives = 99/198 (50.00%), Query Frame = 0


Query: 22  WTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTL-ASLSVQDWRCPICPLASQSSPFQM 81
           W + +V   +L ++RS+E   R+V+  H  L++ L A +   D   P     S ++P Q+
Sbjct: 16  WLSLYVSFCRLNKHRSYEWNCRLVTFTHGILSIGLSAYIGFIDGPWPFTHPGSPNTPLQV 75

Query: 82  QILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSEMVAALLITEI 141
            +L ++  Y I+D+  C + +        HH +SI+GI   L   + G+E+ A L  +EI
Sbjct: 76  HVLCLTLGYFIFDLGWCIYFRSEGPLMLAHHTLSILGIIVALVLGESGTEVNAVLFGSEI 135

Query: 142 SSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLSSNVP-LLIKA 201
           ++P L +R  L+E G+ ++      D  F  +F+  R+  G  L +  + S  P   +K 
Sbjct: 136 TNPLLQIRWFLRETGHYHSFTGDVVDFLFVALFTGVRIGVGACLLFCEMVSPTPKWFVKV 195

Query: 202 MALGLQLVSAYWFYKIVR 218
             + + +VS  + + I R
Sbjct: 196 GGVAMYVVSWCFMFSIWR 213

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BLAST of MS016853.1 vs. ExPASy Swiss-Prot
Match: Q6ZRR5 (TLC domain-containing protein 5 OS=Homo sapiens OX=9606 GN=TLCD5 PE=2 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 1.1e-13
Identity = 54/205 (26.34%), Postives = 100/205 (48.78%), Query Frame = 0


Query: 15  VALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTL-ASLSVQDWRCPICPLAS 74
           V   +  W + ++    L ++RS+E   R+V+  H  L++ L A +   D   P     S
Sbjct: 9   VLCSLCGWLSLYISFCHLNKHRSYEWSCRLVTFTHGVLSIGLSAYIGFIDGPWPFTHPGS 68

Query: 75  QSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSEMVA 134
            ++P Q+ +L ++  Y I+D+  C + +        HH +SI+GI   L   + G+E+ A
Sbjct: 69  PNTPLQVHVLCLTLGYFIFDLGWCVYFQSEGALMLAHHTLSILGIIMALVLGESGTEVNA 128

Query: 135 ALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLSSNV 194
            L  +E+++P L +R  L+E G+ ++      D  F  +F+  R+  G  L +  + S  
Sbjct: 129 VLFGSELTNPLLQMRWFLRETGHYHSFTGDVVDFLFVALFTGVRIGVGACLLFCEMVSPT 188

Query: 195 P-LLIKAMALGLQLVSAYWFYKIVR 218
           P   +KA  + +  VS  + + I R
Sbjct: 189 PKWFVKAGGVAMYAVSWCFMFSIWR 213

[Close]

BLAST of MS016853.1 vs. ExPASy Swiss-Prot
Match: Q3TYE7 (TLC domain-containing protein 5 OS=Mus musculus OX=10090 GN=Tlcd5 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 8.9e-13
Identity = 51/195 (26.15%), Postives = 95/195 (48.72%), Query Frame = 0


Query: 22  WTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTL-ASLSVQDWRCPICPLASQSSPFQM 81
           W + +     L ++RS E   R+V+  H  L++ L A +   D   P     S ++P Q+
Sbjct: 16  WLSLYTSFCCLNKHRSCEWSCRLVTFTHGVLSIGLSAYIGFIDGPWPFTHPGSPNTPLQV 75

Query: 82  QILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSEMVAALLITEI 141
            +L ++  Y I+D+  C + +        HH +SI+GI   LA  + G+E+ A L  +EI
Sbjct: 76  HVLCLTLGYFIFDLGWCIYFQSEGPLMLAHHTLSILGIIMALALGESGTEVNAVLFGSEI 135

Query: 142 SSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLSSNVPLLIKAM 201
           ++P L +R  L+E G+ ++      D  F  +F+  R+  G +L +  + S  P     +
Sbjct: 136 TNPLLQMRWFLRETGHYHSFTGDVVDFLFVALFTGVRIGVGAHLLFCEMVSPTPKWFVKV 195

Query: 202 ALGLQLVSAYWFYKI 216
             G+ + +  W + +
Sbjct: 196 G-GVAMYAVSWCFMV 209

[Close]

BLAST of MS016853.1 vs. ExPASy Swiss-Prot
Match: Q1LXV8 (TLC domain-containing protein 5 OS=Danio rerio OX=7955 GN=tlcd5 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.4e-12
Identity = 57/204 (27.94%), Postives = 97/204 (47.55%), Query Frame = 0


Query: 19  VISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTL-ASLSVQDWRCPICPLASQSSP 78
           +ISW   ++L+  L   R  E   R+V+  H  L + L A +       P     ++++ 
Sbjct: 12  LISWLFLYLLLCHLNSGRDSEWNCRLVTLFHGILIICLTAYIGFIAGPWPFTHPGTENTY 71

Query: 79  FQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSEMVAALLI 138
           FQ+  L +S  Y ++DM  C + +        HH +SI GI   L   + G E  A L  
Sbjct: 72  FQILTLVLSLGYFLFDMAWCVYFRTEGPVMLAHHTMSIFGILLALGLGESGIETCAVLFG 131

Query: 139 TEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLSSNVPLLI 198
           +EI++P L  R  LK +G  ++      D+ F ++F+  R+  G  + Y  L+S  P LI
Sbjct: 132 SEITNPLLQARWFLKRMGCYDSLAGDVVDLLFILLFASVRIGVGSRMLYCELTSPKPSLI 191

Query: 199 KAMALGLQLVSAYWFYKIVRMVRF 222
             +  G+ + +  W + +V + RF
Sbjct: 192 VKVG-GVAIYTLSWIF-MVDIARF 213

[Close]

BLAST of MS016853.1 vs. ExPASy Swiss-Prot
Match: Q5UQN8 (Putative TLC domain-containing protein L438 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L438 PE=4 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.0e-04
Identity = 37/127 (29.13%), Postives = 69/127 (54.33%), Query Frame = 0


Query: 109 IHHLISIVGIAAGLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRN----TELNFA 168
           +HH I IV I           + + A L  E+S+PFL++      I YRN     + +  
Sbjct: 74  VHHFIGIVSIYFSYMKYYYLIKYLFAYLTFELSTPFLNI-----AIKYRNQGVYNKCSIF 133

Query: 169 ADIAFAVIFSFARMVGGPYLTYL---TLSS-----NVPLLIKAMALGLQLVSAYWFYKIV 224
           +++AF ++F+  R++ G YL ++   TLSS     N  +++  +   LQ ++ +W+Y+I+
Sbjct: 134 SELAFFILFTVVRIIFGTYLWFVTSNTLSSIEYPYNYLIVLPTI---LQFLNYWWYYRIL 192

[Close]

BLAST of MS016853.1 vs. ExPASy TrEMBL
Match: A0A6J1CR65 (transmembrane protein 136-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013995 PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 3.2e-122
Identity = 231/232 (99.57%), Postives = 231/232 (99.57%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60
           MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS
Sbjct: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60

Query: 61  VQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120
           VQD RCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA
Sbjct: 61  VQDLRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120

Query: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180
           GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG
Sbjct: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180

Query: 181 GPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN
Sbjct: 181 GPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 232

[Close]

BLAST of MS016853.1 vs. ExPASy TrEMBL
Match: A0A5A7VDR0 (Transmembrane protein 136 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005440 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.8e-104
Identity = 193/233 (82.83%), Postives = 213/233 (91.42%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLL-RNRSFELCNRVVSTLHAALAVTLASL 60
           M VS +RDPD++NVV  GVISWT  FVL+RK++  NRSFE CNR++ST+HA LAVTLAS+
Sbjct: 1   MEVSETRDPDIINVVVYGVISWTLGFVLLRKVVFPNRSFEFCNRLISTIHAFLAVTLASI 60

Query: 61  SVQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIA 120
           SVQ+WRCPICPLAS+SS FQMQ LGVSCSYLIYDMVCCHFDK+VS+DNTIHHL+SIVGIA
Sbjct: 61  SVQNWRCPICPLASKSSSFQMQTLGVSCSYLIYDMVCCHFDKKVSLDNTIHHLVSIVGIA 120

Query: 121 AGLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMV 180
           AG AYQKCGSEMVAAL ITEISSPFLHLREILKE GYR T+LN AADI FAVIFSFARMV
Sbjct: 121 AGFAYQKCGSEMVAALWITEISSPFLHLREILKEFGYRGTDLNLAADIGFAVIFSFARMV 180

Query: 181 GGPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GGPYLTY+TLS+NVP LIKAMALGLQLVSAYWFYKIVRM+RFKL NRSTLKN+
Sbjct: 181 GGPYLTYMTLSANVPFLIKAMALGLQLVSAYWFYKIVRMIRFKLKNRSTLKNS 233

[Close]

BLAST of MS016853.1 vs. ExPASy TrEMBL
Match: A0A1S3BB19 (transmembrane protein 136 OS=Cucumis melo OX=3656 GN=LOC103487998 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.8e-104
Identity = 193/233 (82.83%), Postives = 213/233 (91.42%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLL-RNRSFELCNRVVSTLHAALAVTLASL 60
           M VS +RDPD++NVV  GVISWT  FVL+RK++  NRSFE CNR++ST+HA LAVTLAS+
Sbjct: 1   MEVSETRDPDIINVVVYGVISWTLGFVLLRKVVFPNRSFEFCNRLISTIHAFLAVTLASI 60

Query: 61  SVQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIA 120
           SVQ+WRCPICPLAS+SS FQMQ LGVSCSYLIYDMVCCHFDK+VS+DNTIHHL+SIVGIA
Sbjct: 61  SVQNWRCPICPLASKSSSFQMQTLGVSCSYLIYDMVCCHFDKKVSLDNTIHHLVSIVGIA 120

Query: 121 AGLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMV 180
           AG AYQKCGSEMVAAL ITEISSPFLHLREILKE GYR T+LN AADI FAVIFSFARMV
Sbjct: 121 AGFAYQKCGSEMVAALWITEISSPFLHLREILKEFGYRGTDLNLAADIGFAVIFSFARMV 180

Query: 181 GGPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GGPYLTY+TLS+NVP LIKAMALGLQLVSAYWFYKIVRM+RFKL NRSTLKN+
Sbjct: 181 GGPYLTYMTLSANVPFLIKAMALGLQLVSAYWFYKIVRMIRFKLKNRSTLKNS 233

[Close]

BLAST of MS016853.1 vs. ExPASy TrEMBL
Match: A0A0A0LLI2 (TLC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G362410 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.9e-103
Identity = 192/233 (82.40%), Postives = 212/233 (90.99%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRK-LLRNRSFELCNRVVSTLHAALAVTLASL 60
           M VS SRDPDV+NV+  GVISWT  F+L+RK +  NRSFE CNR++ST+HA LAVTLAS+
Sbjct: 1   MEVSESRDPDVINVMVYGVISWTLGFILLRKAVFPNRSFEFCNRLISTIHAFLAVTLASI 60

Query: 61  SVQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIA 120
           SVQ+WRCPICPLAS+SS FQMQ L VSCSYLIYDMVCCHFDK+VS+DNTIHHL+SIVGIA
Sbjct: 61  SVQNWRCPICPLASKSSSFQMQTLSVSCSYLIYDMVCCHFDKKVSLDNTIHHLVSIVGIA 120

Query: 121 AGLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMV 180
           AGLAYQKCGSEMVAAL ITEISSPFLHLREILKEIGY+ T+LN AADI FAVIFSFARMV
Sbjct: 121 AGLAYQKCGSEMVAALWITEISSPFLHLREILKEIGYKGTDLNLAADIGFAVIFSFARMV 180

Query: 181 GGPYLTYLTLSSNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GGPYLTY+TL +NVP LIKAMALGLQLVSAYWFYKIVRM+RFKLNNRSTLK +
Sbjct: 181 GGPYLTYVTLFANVPFLIKAMALGLQLVSAYWFYKIVRMIRFKLNNRSTLKKS 233

[Close]

BLAST of MS016853.1 vs. ExPASy TrEMBL
Match: A0A6J1HDN8 (transmembrane protein 56-B-like OS=Cucurbita moschata OX=3662 GN=LOC111462571 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.3e-103
Identity = 194/233 (83.26%), Postives = 210/233 (90.13%), Query Frame = 0


Query: 1   MAVSGSRDPDVVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLS 60
           MAVS  RDPDV+NVV LGVI+WT AF+  RKLL NRSFE CNR+VSTLHA LAVTLAS+S
Sbjct: 1   MAVSTGRDPDVINVVVLGVIAWTLAFISFRKLLPNRSFEFCNRLVSTLHACLAVTLASIS 60

Query: 61  VQDWRCPICPLASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAA 120
           V++WRCPICPLASQ+SPFQMQ L V+CSYLIYDMVCCHFDK+V+VDN IHHL+ IVGIAA
Sbjct: 61  VKNWRCPICPLASQASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVCIVGIAA 120

Query: 121 GLAYQKCGSEMVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVG 180
           G AYQKCGSEMVAAL ITEISSPFLHLREILKEIG R T+LN AADI FAVIFSF RMVG
Sbjct: 121 GFAYQKCGSEMVAALWITEISSPFLHLREILKEIGDRGTDLNLAADIGFAVIFSFGRMVG 180

Query: 181 GPYLTYLTLS-SNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTLKNN 233
           GPYLTY+TLS + VPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRST KN+
Sbjct: 181 GPYLTYMTLSATGVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRSTSKNS 233

[Close]

BLAST of MS016853.1 vs. TAIR 10
Match: AT1G35180.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.0e-80
Identity = 141/217 (64.98%), Postives = 179/217 (82.49%), Query Frame = 0


Query: 11  VVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLSVQDWRCPICP 70
           V+++  +GVISW   F+LVR++  + SF+   R+VSTLHA +AVTLA+LS+QDW CP+CP
Sbjct: 8   VISITTIGVISWGLIFILVRRIFSSYSFDFSTRIVSTLHATIAVTLATLSIQDWSCPVCP 67

Query: 71  LASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSE 130
           +AS SS  QM+ L  S SY+IYD++C HFD+ +S+DN +HH + I+G  AGL YQKCGSE
Sbjct: 68  IASTSSLRQMETLAFSLSYMIYDLICSHFDQVLSIDNAVHHSVCILGFVAGLFYQKCGSE 127

Query: 131 MVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLS 190
           MVAAL ITEISSPFLHLREILKEIGYR+T+LN AAD+ FA IFS ARMVGGPYL Y+T+S
Sbjct: 128 MVAALWITEISSPFLHLREILKEIGYRDTDLNLAADVCFATIFSLARMVGGPYLVYVTIS 187

Query: 191 SNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRS 228
           ++ P+LIKAMALGLQLVSA+WFYKI++M+R+K   RS
Sbjct: 188 ADNPILIKAMALGLQLVSAFWFYKILKMMRYKFIKRS 224

[Close]

BLAST of MS016853.1 vs. TAIR 10
Match: AT1G35170.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 272.3 bits (695), Expect = 3.5e-73
Identity = 131/217 (60.37%), Postives = 172/217 (79.26%), Query Frame = 0


Query: 11  VVNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLSVQDWRCPICP 70
           ++++  + +ISW   F+LVR++  + SF+   R+VSTLHA +AVTLA+LS+QDW CP+CP
Sbjct: 8   IISITTIRIISWGLIFILVRRIFSSYSFDFSTRIVSTLHATIAVTLATLSIQDWSCPVCP 67

Query: 71  LASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSE 130
           +AS SS  QM+ L  S SY+IYD++C HFD+ +S+DN +HH + I+G  AGL YQKCGSE
Sbjct: 68  IASTSSLRQMETLAFSLSYMIYDLICSHFDQVLSIDNAVHHSVCILGFVAGLFYQKCGSE 127

Query: 131 MVAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLS 190
           MVAA+ ITEISSPFLHLREILKEIG R+T+LN AAD+ FA IFS ARMVGGPYL Y+T+ 
Sbjct: 128 MVAAIWITEISSPFLHLREILKEIGCRDTDLNLAADVFFATIFSVARMVGGPYLVYVTIP 187

Query: 191 SNVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNNRS 228
           ++ P+LIK M LGLQLVSA  FYK+++M+R+K   RS
Sbjct: 188 ADNPILIKVMGLGLQLVSA--FYKLLKMMRYKFIKRS 222

[Close]

BLAST of MS016853.1 vs. TAIR 10
Match: AT1G45010.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 266.5 bits (680), Expect = 1.9e-71
Identity = 125/212 (58.96%), Postives = 165/212 (77.83%), Query Frame = 0


Query: 12  VNVVALGVISWTTAFVLVRKLLRNRSFELCNRVVSTLHAALAVTLASLSVQDWRCPICPL 71
           + ++  GVISW  AF+L R++  + SF   NR++ST HA +AVTLA+LSVQD  CP+CPL
Sbjct: 9   IKIIVFGVISWGVAFILTRRIFSSYSFSFSNRLLSTAHATIAVTLATLSVQDLSCPVCPL 68

Query: 72  ASQSSPFQMQILGVSCSYLIYDMVCCHFDKRVSVDNTIHHLISIVGIAAGLAYQKCGSEM 131
           AS+ S  QM ++  S SY+IYD++CCHFD+  S+DN +HH +SI+G  AGLAYQK GSE+
Sbjct: 69  ASKPSHKQMDVMAFSLSYMIYDLICCHFDQVFSIDNAVHHFVSILGFIAGLAYQKSGSEI 128

Query: 132 VAALLITEISSPFLHLREILKEIGYRNTELNFAADIAFAVIFSFARMVGGPYLTYLTLSS 191
           VA L + EISSPF HLREILKEIGY++T+LN AAD+ FA IF+ AR+V GP+L Y++LS+
Sbjct: 129 VATLWVAEISSPFFHLREILKEIGYKDTKLNLAADVCFATIFTLARIVCGPFLVYVSLSA 188

Query: 192 NVPLLIKAMALGLQLVSAYWFYKIVRMVRFKL 224
           + P+ IKAM  GLQLVS +WFYKI  M+R+KL
Sbjct: 189 DNPIFIKAMGSGLQLVSIFWFYKIFGMMRYKL 220

The following BLAST results are available for this feature:

BLAST of MS016853.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144270.1	6.6e-122	99.57	transmembrane protein 136-like isoform X1 [Momordica charantia]	[more]
XP_038886579.1	9.6e-105	82.76	TLC domain-containing protein 5-like [Benincasa hispida]	[more]
KAG7029404.1	2.1e-104	84.12	Transmembrane protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6598466.1	2.8e-104	83.69	TLC domain-containing protein 5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_008444752.1	3.6e-104	82.83	PREDICTED: transmembrane protein 136 [Cucumis melo] >KAA0065207.1 transmembrane ...	[more]

BLAST of MS016853.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0VD42	1.1e-13	27.27	TLC domain-containing protein 5 OS=Bos taurus OX=9913 GN=TLCD5 PE=2 SV=1	[more]
Q6ZRR5	1.1e-13	26.34	TLC domain-containing protein 5 OS=Homo sapiens OX=9606 GN=TLCD5 PE=2 SV=2	[more]
Q3TYE7	8.9e-13	26.15	TLC domain-containing protein 5 OS=Mus musculus OX=10090 GN=Tlcd5 PE=2 SV=1	[more]
Q1LXV8	4.4e-12	27.94	TLC domain-containing protein 5 OS=Danio rerio OX=7955 GN=tlcd5 PE=2 SV=1	[more]
Q5UQN8	2.0e-04	29.13	Putative TLC domain-containing protein L438 OS=Acanthamoeba polyphaga mimivirus ...	[more]

BLAST of MS016853.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CR65	3.2e-122	99.57	transmembrane protein 136-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A5A7VDR0	1.8e-104	82.83	Transmembrane protein 136 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A1S3BB19	1.8e-104	82.83	transmembrane protein 136 OS=Cucumis melo OX=3656 GN=LOC103487998 PE=4 SV=1	[more]
A0A0A0LLI2	1.9e-103	82.40	TLC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G362410 PE=4 SV...	[more]
A0A6J1HDN8	4.3e-103	83.26	transmembrane protein 56-B-like OS=Cucurbita moschata OX=3662 GN=LOC111462571 PE...	[more]

BLAST of MS016853.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G35180.1	1.0e-80	64.98	TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein	[more]
AT1G35170.1	3.5e-73	60.37	TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein	[more]
AT1G45010.1	1.9e-71	58.96	TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006634	TRAM/LAG1/CLN8 homology domain	SMART	SM00724	lag1_27	coord: 35..224 e-value: 1.2E-40 score: 151.1
IPR006634	TRAM/LAG1/CLN8 homology domain	PFAM	PF03798	TRAM_LAG1_CLN8	coord: 41..217 e-value: 7.5E-24 score: 84.6
IPR006634	TRAM/LAG1/CLN8 homology domain	PROSITE	PS50922	TLC	coord: 35..224 score: 36.969093
IPR042512	TLC domain-containing protein 5	PANTHER	PTHR31898	TRANSMEMBRANE PROTEIN 136	coord: 10..227
None	No IPR available	PANTHER	PTHR31898:SF5	SUBFAMILY NOT NAMED	coord: 10..227

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS016853	MS016853	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS016853.1-cds	MS016853.1-cds-scaffold9_1:493985..494221	CDS
MS016853.1-cds	MS016853.1-cds-scaffold9_1:494404..494544	CDS
MS016853.1-cds	MS016853.1-cds-scaffold9_1:494725..494844	CDS
MS016853.1-cds	MS016853.1-cds-scaffold9_1:495355..495450	CDS
MS016853.1-cds	MS016853.1-cds-scaffold9_1:495675..495776	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS016853.1	MS016853.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane