MS016791.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS016791.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAllene oxide synthase
Locationscaffold9_1: 51105 .. 52694 (-)
Sequence length1590
RNA-Seq ExpressionMS016791.1
SyntenyMS016791.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGCTACCTCCATAACCTTGCCTTGTCTTCAACCCCAGATCCGATTCCCGTCGCAGAAGACGCCGGCTAGGGCCCGTTCTAATGTCCGGCTTGCTTCCGTACGGCCGATCTGCGCCGCCGAGAAGGCGTCCGCGGCGTCCCCTGTTCAAGCTCCGCAGCGGACGGTTTCGCCGCCGGAACGGACGAAGCTGCCGCTGAGGAAGATTCCCGGCGACTATGGGCCGCCGGTTGTCGGCCCGTTGAGGGACCGGCAGGACTATTTCTACAATCAGGGGAGGGAAGAGTATTTGAAATCTCGAATGCTTAGGTACGAATCGACTGTGTACAGGACCAATATGCCGCCGGGTCCTTTCATCTCCTCCGATCCTCGCGTCGTCGTTTTACTCGACGGCAAGAGCTTCCCTGTACTTTTTGACGATTCTAAAGTCGAGAAGAAGGACCTCTTCACCGGAACTTACATGCCTTCAACAGAACTCACCGGCGGCTACCGGGTTCTCTCTTATCTCGACCCCTCGGAGCCCAATCACGAGAAGCTCAAGCAACTCGTCTTCTTCCTCCTCAAGAACAGCCGCGACAAAATTCTCCCGGAGTTTCATTCCACCTTTTCGGAGCTGTTCGAGACTCTGGAGAAGGAGGTGGCCACCGCCGGTAGAGCAGGGTACAATGCCCACGGCGAACAGGCGGCGTTTAATTTCCTGGCTCGGTCGCTCTTCGGCGCGAACCCGGCGGACACCAAATTAGGCCGCGACGCTCCCAAATTGATTTCGAAATGGGTGCTGTTTCAACTGGGTCCGGTTCTCACCCTCGGCCTCCCCAAGCCCGTGGAGGAGCTCCTCCTCCGCACCGTCCGCCTCCCGCCGGCGCTGATCAAGGCCGATTACAAGCGGCTGTACGACTTCTTCTACGAGTCGTCGGAGGCTGTGTTTGCAGAGGCGGACAGATTAGGGATTTCGAGGGAGGAGGCGTGCCATAACCTGATTTTCACCACGTGTTTCAATTCGTTCGGAGGGATGAAGATTCTGTTTCCCAATATGATAAAATGGATCGGACGTGCCGGAGCGAATCTTCACACGCGACTTGCACGCGAGATACGATCCGCCGTTGAGGCCCACGGCGGGAAGATCACGATGGCCGCCATGGAACAGATGCCGCTGATGAAATCGGTTGTGTACGAAGCGTTCAGAATGGAGCCTCCGGTGCCGTTCCAGTACGGGCGAGCGAAAAGGGACATCGTGATCGAGAGCCACGACGCCGCTTTCGAGATCAAAGAAGGGGAAATGATCTGTGGGTACCAGCCCTTCGCCACCAGAGATCCGAAGATTTTCGACAGACCCAACGAATTCGTGCCGGATCGGTTCATCGGGGACGGCGAGGAGTTGCTCAAGCACGTGCTCTGGTCGAACGGACCGGAGACTCAATCGCCGTCGGTTAAAAACAAGCAGTGCGCCGGAAAGGACTTCGTCGTCTTCGCGTCGCGGCTTCTCGTCGTCGAACTCTTCCTCCGCTACGATTCCTTCGACATCGAAGTCGGAGCCTCGCCGTTGGGCGCCGCCGTCACCGTAACGTCTCTGAAGAAGGCAAGCTTC

mRNA sequence

ATGTCGGCTACCTCCATAACCTTGCCTTGTCTTCAACCCCAGATCCGATTCCCGTCGCAGAAGACGCCGGCTAGGGCCCGTTCTAATGTCCGGCTTGCTTCCGTACGGCCGATCTGCGCCGCCGAGAAGGCGTCCGCGGCGTCCCCTGTTCAAGCTCCGCAGCGGACGGTTTCGCCGCCGGAACGGACGAAGCTGCCGCTGAGGAAGATTCCCGGCGACTATGGGCCGCCGGTTGTCGGCCCGTTGAGGGACCGGCAGGACTATTTCTACAATCAGGGGAGGGAAGAGTATTTGAAATCTCGAATGCTTAGGTACGAATCGACTGTGTACAGGACCAATATGCCGCCGGGTCCTTTCATCTCCTCCGATCCTCGCGTCGTCGTTTTACTCGACGGCAAGAGCTTCCCTGTACTTTTTGACGATTCTAAAGTCGAGAAGAAGGACCTCTTCACCGGAACTTACATGCCTTCAACAGAACTCACCGGCGGCTACCGGGTTCTCTCTTATCTCGACCCCTCGGAGCCCAATCACGAGAAGCTCAAGCAACTCGTCTTCTTCCTCCTCAAGAACAGCCGCGACAAAATTCTCCCGGAGTTTCATTCCACCTTTTCGGAGCTGTTCGAGACTCTGGAGAAGGAGGTGGCCACCGCCGGTAGAGCAGGGTACAATGCCCACGGCGAACAGGCGGCGTTTAATTTCCTGGCTCGGTCGCTCTTCGGCGCGAACCCGGCGGACACCAAATTAGGCCGCGACGCTCCCAAATTGATTTCGAAATGGGTGCTGTTTCAACTGGGTCCGGTTCTCACCCTCGGCCTCCCCAAGCCCGTGGAGGAGCTCCTCCTCCGCACCGTCCGCCTCCCGCCGGCGCTGATCAAGGCCGATTACAAGCGGCTGTACGACTTCTTCTACGAGTCGTCGGAGGCTGTGTTTGCAGAGGCGGACAGATTAGGGATTTCGAGGGAGGAGGCGTGCCATAACCTGATTTTCACCACGTGTTTCAATTCGTTCGGAGGGATGAAGATTCTGTTTCCCAATATGATAAAATGGATCGGACGTGCCGGAGCGAATCTTCACACGCGACTTGCACGCGAGATACGATCCGCCGTTGAGGCCCACGGCGGGAAGATCACGATGGCCGCCATGGAACAGATGCCGCTGATGAAATCGGTTGTGTACGAAGCGTTCAGAATGGAGCCTCCGGTGCCGTTCCAGTACGGGCGAGCGAAAAGGGACATCGTGATCGAGAGCCACGACGCCGCTTTCGAGATCAAAGAAGGGGAAATGATCTGTGGGTACCAGCCCTTCGCCACCAGAGATCCGAAGATTTTCGACAGACCCAACGAATTCGTGCCGGATCGGTTCATCGGGGACGGCGAGGAGTTGCTCAAGCACGTGCTCTGGTCGAACGGACCGGAGACTCAATCGCCGTCGGTTAAAAACAAGCAGTGCGCCGGAAAGGACTTCGTCGTCTTCGCGTCGCGGCTTCTCGTCGTCGAACTCTTCCTCCGCTACGATTCCTTCGACATCGAAGTCGGAGCCTCGCCGTTGGGCGCCGCCGTCACCGTAACGTCTCTGAAGAAGGCAAGCTTC

Coding sequence (CDS)

ATGTCGGCTACCTCCATAACCTTGCCTTGTCTTCAACCCCAGATCCGATTCCCGTCGCAGAAGACGCCGGCTAGGGCCCGTTCTAATGTCCGGCTTGCTTCCGTACGGCCGATCTGCGCCGCCGAGAAGGCGTCCGCGGCGTCCCCTGTTCAAGCTCCGCAGCGGACGGTTTCGCCGCCGGAACGGACGAAGCTGCCGCTGAGGAAGATTCCCGGCGACTATGGGCCGCCGGTTGTCGGCCCGTTGAGGGACCGGCAGGACTATTTCTACAATCAGGGGAGGGAAGAGTATTTGAAATCTCGAATGCTTAGGTACGAATCGACTGTGTACAGGACCAATATGCCGCCGGGTCCTTTCATCTCCTCCGATCCTCGCGTCGTCGTTTTACTCGACGGCAAGAGCTTCCCTGTACTTTTTGACGATTCTAAAGTCGAGAAGAAGGACCTCTTCACCGGAACTTACATGCCTTCAACAGAACTCACCGGCGGCTACCGGGTTCTCTCTTATCTCGACCCCTCGGAGCCCAATCACGAGAAGCTCAAGCAACTCGTCTTCTTCCTCCTCAAGAACAGCCGCGACAAAATTCTCCCGGAGTTTCATTCCACCTTTTCGGAGCTGTTCGAGACTCTGGAGAAGGAGGTGGCCACCGCCGGTAGAGCAGGGTACAATGCCCACGGCGAACAGGCGGCGTTTAATTTCCTGGCTCGGTCGCTCTTCGGCGCGAACCCGGCGGACACCAAATTAGGCCGCGACGCTCCCAAATTGATTTCGAAATGGGTGCTGTTTCAACTGGGTCCGGTTCTCACCCTCGGCCTCCCCAAGCCCGTGGAGGAGCTCCTCCTCCGCACCGTCCGCCTCCCGCCGGCGCTGATCAAGGCCGATTACAAGCGGCTGTACGACTTCTTCTACGAGTCGTCGGAGGCTGTGTTTGCAGAGGCGGACAGATTAGGGATTTCGAGGGAGGAGGCGTGCCATAACCTGATTTTCACCACGTGTTTCAATTCGTTCGGAGGGATGAAGATTCTGTTTCCCAATATGATAAAATGGATCGGACGTGCCGGAGCGAATCTTCACACGCGACTTGCACGCGAGATACGATCCGCCGTTGAGGCCCACGGCGGGAAGATCACGATGGCCGCCATGGAACAGATGCCGCTGATGAAATCGGTTGTGTACGAAGCGTTCAGAATGGAGCCTCCGGTGCCGTTCCAGTACGGGCGAGCGAAAAGGGACATCGTGATCGAGAGCCACGACGCCGCTTTCGAGATCAAAGAAGGGGAAATGATCTGTGGGTACCAGCCCTTCGCCACCAGAGATCCGAAGATTTTCGACAGACCCAACGAATTCGTGCCGGATCGGTTCATCGGGGACGGCGAGGAGTTGCTCAAGCACGTGCTCTGGTCGAACGGACCGGAGACTCAATCGCCGTCGGTTAAAAACAAGCAGTGCGCCGGAAAGGACTTCGTCGTCTTCGCGTCGCGGCTTCTCGTCGTCGAACTCTTCCTCCGCTACGATTCCTTCGACATCGAAGTCGGAGCCTCGCCGTTGGGCGCCGCCGTCACCGTAACGTCTCTGAAGAAGGCAAGCTTC

Protein sequence

MSATSITLPCLQPQIRFPSQKTPARARSNVRLASVRPICAAEKASAASPVQAPQRTVSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF
Homology
BLAST of MS016791.1 vs. NCBI nr
Match: XP_038885277.1 (allene oxide synthase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 913.7 bits (2360), Expect = 7.4e-262
Identity = 451/534 (84.46%), Postives = 490/534 (91.76%), Query Frame = 0

Query: 1   MSATSITLPCLQPQIRFPSQKTPARARSNVRLASVRPICAAEK----ASAASPVQAPQRT 60
           MS+TSIT+PCLQP +RFP Q+ P R+RS V   S+RPI AA++    +S++S VQ PQRT
Sbjct: 1   MSSTSITIPCLQPPLRFPYQEPPPRSRSRVGFVSLRPIYAADRVSSSSSSSSSVQVPQRT 60

Query: 61  VSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPP 120
           VS PE TKLPLRKIPGDYGPPV G L+DRQDYFYNQG+EEYLKSRMLRYESTVYRTNMPP
Sbjct: 61  VSTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGKEEYLKSRMLRYESTVYRTNMPP 120

Query: 121 GPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPN 180
           GPFI+SD RVVVLLDGKSFPVLFD SKVEKKDLFTGTYMP TELTGG+RVLSY+DPSEP+
Sbjct: 121 GPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGHRVLSYIDPSEPD 180

Query: 181 HEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLAR 240
           H KLKQL+FFLLK+ RDKILPEFHSTFSELFETLEK++A AGRA YNA GEQAAFNFLAR
Sbjct: 181 HAKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGRAQYNAPGEQAAFNFLAR 240

Query: 241 SLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYK 300
           SLFGANP D+KLGRDAPKLI KWVLFQLGPVL+LGLPK VEELLLRTVRLPPALIKADY+
Sbjct: 241 SLFGANPVDSKLGRDAPKLIGKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYR 300

Query: 301 RLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGAN 360
           RLYDFFYESSEAVF EADRLGISREEACHNL+F TCFNSFGGMKI FPNMIKWIGRAG  
Sbjct: 301 RLYDFFYESSEAVFEEADRLGISREEACHNLLFATCFNSFGGMKIFFPNMIKWIGRAGVK 360

Query: 361 LHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIES 420
           LHT+LAREIR+AV+++GGKITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+ES
Sbjct: 361 LHTQLAREIRTAVKSNGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVES 420

Query: 421 HDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSP 480
           HDAAFEIKEGE+ICGYQPFATRDPKIFDR  EFVPDRF GDGEELLKHVLWSNGPETQSP
Sbjct: 421 HDAAFEIKEGEVICGYQPFATRDPKIFDRAGEFVPDRFTGDGEELLKHVLWSNGPETQSP 480

Query: 481 SVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           +V+NKQCAGKDF+VF SRLLVVELFLRYDSFDIE   +PLGAAVTVTSLKKASF
Sbjct: 481 TVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 534

BLAST of MS016791.1 vs. NCBI nr
Match: KAG7029361.1 (Allene oxide synthase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 907.1 bits (2343), Expect = 7.0e-260
Identity = 449/536 (83.77%), Postives = 485/536 (90.49%), Query Frame = 0

Query: 1   MSATSITLPCLQPQIRFPSQKTPARARSNVRLASVRPICAAEKASAA------SPVQAPQ 60
           MS TSITLPC QP  RFPSQK P R+RSN+   S R I AA+  S++      S VQ PQ
Sbjct: 1   MSVTSITLPCPQPHSRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQ 60

Query: 61  RTVSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           R    PE TKLPLRKIPGDYGPPV G L+DRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 61  RIAPTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120

Query: 121 PPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSE 180
           PPGPFI++D RVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSY+DPSE
Sbjct: 121 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 180

Query: 181 PNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFL 240
           P+HEKLKQL+FFLLK+ RDKILPEFHSTFSELF+TLEK++A  GRA YNA GEQAAFNFL
Sbjct: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 240

Query: 241 ARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKAD 300
           ARSLFGANP DTKLGRDAPKLI+KWVLFQLGPVL LGLPK VEELLLRTVRLPP+LIKAD
Sbjct: 241 ARSLFGANPVDTKLGRDAPKLIAKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 300

Query: 301 YKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAG 360
           Y+RLYDFFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG
Sbjct: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360

Query: 361 ANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVI 420
           ANLHT+LAREIR+AV+A+GG+ITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+
Sbjct: 361 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420

Query: 421 ESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGEMICG+QPFATRDPKIFDR NEFVPDRF GDGE LLKHVLWSNGPETQ
Sbjct: 421 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRANEFVPDRFTGDGERLLKHVLWSNGPETQ 480

Query: 481 SPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           SP+V+NKQCAGKDF+VF SRL VVE FLRYDSFDIEV  SPLGAA+TVTSLKKASF
Sbjct: 481 SPTVQNKQCAGKDFIVFISRLFVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of MS016791.1 vs. NCBI nr
Match: XP_023547133.1 (allene oxide synthase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.7 bits (2342), Expect = 9.1e-260
Identity = 449/536 (83.77%), Postives = 486/536 (90.67%), Query Frame = 0

Query: 1   MSATSITLPCLQPQIRFPSQKTPARARSNVRLASVRPICAAEKASAA------SPVQAPQ 60
           MS TSITLPC QP  RFPSQK P R+RSN+   S R I AA+  S++      S VQ PQ
Sbjct: 1   MSVTSITLPCPQPHSRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQ 60

Query: 61  RTVSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           R    PE TKLPLRKIPGDYGPPV G L+DRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 61  RIAPTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120

Query: 121 PPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSE 180
           PPGPFI++D RVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSY+DPSE
Sbjct: 121 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 180

Query: 181 PNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFL 240
           P+HEKLKQL+FFLLK+ RDKILPEFHSTFSELF+TLEK++A  GRA YNA GEQAAFNFL
Sbjct: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 240

Query: 241 ARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKAD 300
           ARSLFGANP DTKLGRDAPKLI+KWVLFQLGPVL LGLPK VEELLLRTVRLPP+LIKAD
Sbjct: 241 ARSLFGANPVDTKLGRDAPKLIAKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 300

Query: 301 YKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAG 360
           Y+RLYDFFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG
Sbjct: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360

Query: 361 ANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVI 420
           ANLHT+LAREIR+AV+A+GG+ITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+
Sbjct: 361 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420

Query: 421 ESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGEMICG+QPFATRDPKIFDR +EFVPDRF GDGE LLKHVLWSNGPETQ
Sbjct: 421 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQ 480

Query: 481 SPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           SP+V+NKQCAGKDF+VF SRLLVVE FLRYDSFDIEV  SPLGAA+TVTSLKKASF
Sbjct: 481 SPTVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of MS016791.1 vs. NCBI nr
Match: KAA0065268.1 (allene oxide synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 906.4 bits (2341), Expect = 1.2e-259
Identity = 446/530 (84.15%), Postives = 489/530 (92.26%), Query Frame = 0

Query: 4   TSITLPCLQPQIRFP-SQKTPARARSNVRLASVRPICAAE--KASAASPVQAPQRTVSPP 63
           +SI +P LQP +RFP SQ+TP R+RS V   S+RPI AA+   +S++S +Q PQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYAADGVSSSSSSSLQVPQRIVSPP 61

Query: 64  ERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           E TKLPLRK+PGDYGPP+ G L+DR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 SSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEKL 183
           +SD RVVVLLDGKSFPVLFD SKVEKKDLFTGTYMP TELTGGYRVLSY+DPSEP+H KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLFG 243
           KQL+FFLLK+ RDKI+PEFHSTFSELFETLEK++A AGRA YNA GEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYKRLYD 303
           A+P D+KLGRDAPKLI+KWVLFQLGPVL+LGLPK VEELLLRTVRLPPALIKADY+RLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHTR 363
           FFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG NLHTR
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDAA 423
           LAREIR+AV+A+GGKITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+ESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSPSVKN 483
           FEIKEGE+ICGYQPFATRDPKIFDR +EFVPDRF G+GEELLKHV+WSNGPETQSPSV+N
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           KQCAGKDF+VF SRLLVVELFLRYDSFDIE   +PLGAAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of MS016791.1 vs. NCBI nr
Match: XP_022962362.1 (allene oxide synthase 1, chloroplastic-like [Cucurbita moschata] >KAG6598409.1 Allene oxide synthase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 906.0 bits (2340), Expect = 1.5e-259
Identity = 448/536 (83.58%), Postives = 485/536 (90.49%), Query Frame = 0

Query: 1   MSATSITLPCLQPQIRFPSQKTPARARSNVRLASVRPICAAEKASAA------SPVQAPQ 60
           MS TSITLPC QP  RFPSQK P R+RSN+   S R I AA+  S++      S VQ PQ
Sbjct: 1   MSVTSITLPCPQPHSRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQ 60

Query: 61  RTVSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           R    PE TKLPLRKIPGDYGPP+ G L+DRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 61  RIAPTPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120

Query: 121 PPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSE 180
           PPGPFI++D RVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSY+DPSE
Sbjct: 121 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 180

Query: 181 PNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFL 240
           P+HEKLKQL+FFLLK+ RDKILPEFHSTFSELF+TLEK++A  GRA YNA GEQAAFNFL
Sbjct: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 240

Query: 241 ARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKAD 300
           ARSLFGANP DTKLGRDAPKLI+KWVLFQLGPVL LGLPK VEELLLRTVRLPP+LIKAD
Sbjct: 241 ARSLFGANPVDTKLGRDAPKLIAKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 300

Query: 301 YKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAG 360
           Y+RLYDFFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG
Sbjct: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360

Query: 361 ANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVI 420
           ANLHT+LAREIR+AV+A+GG+ITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+
Sbjct: 361 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420

Query: 421 ESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGEMICG+QPFATRDPKIFDR NEFVPDRF GDGE LLKHVLWSNGPETQ
Sbjct: 421 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRANEFVPDRFTGDGERLLKHVLWSNGPETQ 480

Query: 481 SPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           SP+V+NKQCAGKDF+VF SRL VVE FLRYDSFDIEV  SPLGAA+TVTSLKKASF
Sbjct: 481 SPTVQNKQCAGKDFIVFISRLFVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of MS016791.1 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 1.4e-215
Identity = 366/536 (68.28%), Postives = 456/536 (85.07%), Query Frame = 0

Query: 1   MSATSITLPCLQPQIRFPSQ-KTPARARSNVRLASVRPICAAEK---ASAASPVQAPQRT 60
           M++TS++LP L  +++FPS   + +R  S+    S+RPI A+        +SP Q+P  +
Sbjct: 1   MASTSLSLPSL--KLQFPSHTSSSSRKNSSSYRVSIRPIQASVSEIPPYISSPSQSPSSS 60

Query: 61  VSPP-ERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMP 120
            SPP ++ KLP +K+PGDYG P+VGP +DR DYFYNQG+ E+ KSR+ +++STV+RTNMP
Sbjct: 61  SSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRTNMP 120

Query: 121 PGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEP 180
           PGPFIS +P VVVLLDGKSFPVLFD SKVEKKDLFTGT+MPST+LTGGYRVLSYLDPSEP
Sbjct: 121 PGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPSEP 180

Query: 181 NHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLA 240
           NH KLK+L+F+LL + R++++PEFH+++SELFETLE E++T G+AG NA  +QAAFNFLA
Sbjct: 181 NHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAGLNAANDQAAFNFLA 240

Query: 241 RSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADY 300
           RSL+G NP DT+LG D PKLI KWVLFQL P+L LGLPK +E+L++ T RLPPAL+K DY
Sbjct: 241 RSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKVLEDLVMHTFRLPPALVKKDY 300

Query: 301 KRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGA 360
           +RLY+FFYE+S +V  EA+++GISREEACHNL+F TCFNSFGG+KI FPNM+KWIGRAGA
Sbjct: 301 QRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKWIGRAGA 360

Query: 361 NLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIE 420
            LH++LA+EIRS + ++ GK+TMAAME+MPLMKSVVYE+ R+EPPV  QYGRAK D+VIE
Sbjct: 361 KLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAKHDMVIE 420

Query: 421 SHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIG-DGEELLKHVLWSNGPETQ 480
           SHDA+FEIKEGE++ GYQPFAT+DPKIFDR  EFV DRFIG +GE+LLKHVLWSNG ET+
Sbjct: 421 SHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWSNGSETE 480

Query: 481 SPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           + S+ NKQCAGKDFVV  SRLL+VELFLRYDSF+IEVGASPLGAA+T+TSL++ASF
Sbjct: 481 NASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRASF 534

BLAST of MS016791.1 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 3.1e-202
Identity = 341/530 (64.34%), Postives = 431/530 (81.32%), Query Frame = 0

Query: 8   LPCLQPQIRFPSQKT---PARARSNVRLASVRPICAAEKASAASPVQAPQRTVSPP---E 67
           L  + P    PS K+   P    +  R+++ RPI A+      SP++ P  T  PP   +
Sbjct: 9   LVAVNPNTLSPSPKSTPLPNTFSNLRRVSAFRPIKAS--LFGDSPIKIPGITSQPPPSSD 68

Query: 68  RTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFIS 127
            T LP+R+IPGDYG P +GP++DR DYFYNQGREE+ KSR+ +Y+STVYR NMPPGPFI+
Sbjct: 69  ETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKSTVYRANMPPGPFIA 128

Query: 128 SDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEKLK 187
           S+PRV+VLLD KSFPVLFD SKVEKKDLFTGTYMPSTELTGGYR+LSYLDPSEPNH KLK
Sbjct: 129 SNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHTKLK 188

Query: 188 QLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLFGA 247
           QL+F L+KN RD ++PEF S+F++L E +E ++AT G+A +N   EQAAFNFL+R+ FG 
Sbjct: 189 QLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAAFNDPAEQAAFNFLSRAFFGV 248

Query: 248 NPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYKRLYDF 307
            P DT LG+DAP LISKWVLF L P+L++GLPK VEE  L +VRLPP L++ DY RLY+F
Sbjct: 249 KPIDTPLGKDAPSLISKWVLFNLAPILSVGLPKEVEEATLHSVRLPPLLVQNDYHRLYEF 308

Query: 308 FYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHTRL 367
           F  ++ +V  EA++ GISR+EACHN++F  CFNS+GG KILFP+++KWIGRAG  LHT+L
Sbjct: 309 FTSAAGSVLDEAEQSGISRDEACHNILFAVCFNSWGGFKILFPSLMKWIGRAGLELHTKL 368

Query: 368 AREIRSAVEA-HGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDAA 427
           A+EIRSA+++  GGK+TMAAMEQMPLMKSVVYE  R+EPPV  QYG+AK+D ++ESH+AA
Sbjct: 369 AQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQYGKAKKDFILESHEAA 428

Query: 428 FEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSPSVKN 487
           +++KEGEM+ GYQPFAT+DPKIFDRP EFV DRF+G+G +L+++V+WSNGPET++PSV N
Sbjct: 429 YQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVGEGVKLMEYVMWSNGPETETPSVAN 488

Query: 488 KQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           KQCAGKDFVV A+RL VVELF RYDSFDIEVG S LGA++T+TSLK+++F
Sbjct: 489 KQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASITLTSLKRSTF 536

BLAST of MS016791.1 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 3.8e-200
Identity = 325/474 (68.57%), Postives = 402/474 (84.81%), Query Frame = 0

Query: 56  TVSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMP 115
           T+   +  KL  R IPGDYG P +GP +DR DYFYNQG+ ++ +SR+ +Y+ST++RTNMP
Sbjct: 37  TIEITQPIKLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMP 96

Query: 116 PGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEP 175
           PGPFI+S+P+V+VLLDGKSFPVLFD SKVEKKDLFTGT++PSTELTGGYR+LSYLDPSEP
Sbjct: 97  PGPFITSNPKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEP 156

Query: 176 NHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLA 235
           NHEKLK+L+FFLL + RD ++PEFH T++ELFETL+KE+   G  G+N+  +QAAFNFLA
Sbjct: 157 NHEKLKKLMFFLLSSRRDHVIPEFHETYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLA 216

Query: 236 RSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADY 295
           RSLFG NP +TKLG D P LI KW+L QL PV+TLGLPK ++++LL T RLPP L+K DY
Sbjct: 217 RSLFGVNPVETKLGTDGPALIGKWILLQLHPVITLGLPKFLDDVLLHTFRLPPILVKKDY 276

Query: 296 KRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGA 355
           +RLYDFFY +S  +F EA++LGIS++EACHNL+F TCFNSFGGMKI FPNM+K I +AG 
Sbjct: 277 QRLYDFFYTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGV 336

Query: 356 NLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIE 415
            +HTRLA EIRS V++ GGKITM+AME+MPLMKSVVYEA R++PPV  QYGRAK+D+ IE
Sbjct: 337 EIHTRLANEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIE 396

Query: 416 SHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIG-DGEELLKHVLWSNGPETQ 475
           SHDA FE+K+GE++ GYQPFAT+DPKIFDRP EFV DRF+G +GE+LLKHVLWSNGPET+
Sbjct: 397 SHDAVFEVKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETE 456

Query: 476 SPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKA 529
           SP+V NKQCAGKDFVV  SRL V E FLRY + +++VG S LG+++T+TSLKKA
Sbjct: 457 SPTVGNKQCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

BLAST of MS016791.1 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 682.9 bits (1761), Expect = 2.8e-195
Identity = 325/486 (66.87%), Postives = 404/486 (83.13%), Query Frame = 0

Query: 47  ASPVQAPQRTVSPPERTK-LPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRY 106
           AS  + P  TV+    +K LP+R IPG+YG P+VGP++DR DYFY+QG EE+ KSR+ +Y
Sbjct: 33  ASGSETPDLTVATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKY 92

Query: 107 ESTVYRTNMPPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYR 166
            STVYR NMPPG FI+ +P+VV LLDGKSFPVLFD  KVEKKDLFTGTYMPSTELTGGYR
Sbjct: 93  NSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYR 152

Query: 167 VLSYLDPSEPNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAH 226
           +LSYLDPSEP HEKLK L+FFLLK+SR++I PEF +T+SELF++LEKE++  G+A +   
Sbjct: 153 ILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGS 212

Query: 227 GEQAAFNFLARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVR 286
            +  AFNFLAR+ +G NPADTKL  DAP LI+KWVLF L P+L++GLP+ +EE L+ T  
Sbjct: 213 SDGTAFNFLARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFS 272

Query: 287 LPPALIKADYKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPN 346
           LPPAL+K+DY+RLY+FF ES+  +  EAD+LGISREEA HNL+F TCFN++GGMKILFPN
Sbjct: 273 LPPALVKSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPN 332

Query: 347 MIKWIGRAGANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQY 406
           M+K IGRAG  +H RLA EIRS ++++GG++TM A+E+M L KSVVYE  R EPPV  QY
Sbjct: 333 MVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQY 392

Query: 407 GRAKRDIVIESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIG-DGEELLKH 466
           GRAK+D+VIESHDAAF++K GEM+ GYQP ATRDPKIFDR +EFVP+RF+G +GE+LL+H
Sbjct: 393 GRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRH 452

Query: 467 VLWSNGPETQSPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTS 526
           VLWSNGPET++P+V NKQCAGKDFVV  +RL V+E+F RYDSFDIEVG SPLG++V  +S
Sbjct: 453 VLWSNGPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSS 512

Query: 527 LKKASF 531
           L+KASF
Sbjct: 513 LRKASF 518

BLAST of MS016791.1 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 648.7 bits (1672), Expect = 5.8e-185
Identity = 304/466 (65.24%), Postives = 382/466 (81.97%), Query Frame = 0

Query: 66  PLRKIPGDYGPPVVGPLRDRQDYFYNQ-GREEYLKSRMLRYESTVYRTNMPPGPFISSDP 125
           PLR+IPG YG P   P++DR +YFY   GR+EY +SRM +Y+STV+R NMPPGPF+SS+P
Sbjct: 7   PLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPFVSSNP 66

Query: 126 RVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEKLKQLV 185
           +V+VLLD KSFP+LFD SKVEKKDLFTGTYMPST+LTGGYRVLSYLDPSEP H +LK L+
Sbjct: 67  KVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQLKNLL 126

Query: 186 FFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLFGANPA 245
           FF+LKNS ++++P+F +T++ELFE LE E+A  G+A +N  GEQAAF FL R+ F +NP 
Sbjct: 127 FFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYFNSNPE 186

Query: 246 DTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYKRLYDFFYE 305
           +TKLG  AP LIS WVLF L P L LGLP  ++E LL T RLP  LIK+ Y +LYD+F  
Sbjct: 187 ETKLGTSAPTLISSWVLFNLAPTLDLGLPWFLQEPLLHTFRLPAFLIKSTYNKLYDYFQS 246

Query: 306 SSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHTRLARE 365
            +  V  +A++LG+ ++EA HN++F  CFN+FGG+KILFPN +KWIG AG NLHT+LA E
Sbjct: 247 VATPVMEQAEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENLHTQLAEE 306

Query: 366 IRSAVEAHG-GKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDAAFEI 425
           IR A++++G G +T+ A+EQMPL KSVVYE+ R+EPPVP QYG+AK +  IESHDA FE+
Sbjct: 307 IRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHDATFEV 366

Query: 426 KEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSPSVKNKQC 485
           K+GEM+ GYQPFAT+DPK+FDRP E+VPDRF+GDGE LLK+V WSNGPET+SP+V+NKQC
Sbjct: 367 KKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGDGEALLKYVWWSNGPETESPTVENKQC 426

Query: 486 AGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKAS 530
           AGKDFVV  +RL V+ELF RYDSF+IE+G SPLGAAVT+T LK+AS
Sbjct: 427 AGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRAS 472

BLAST of MS016791.1 vs. ExPASy TrEMBL
Match: A0A5A7VCB0 (Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G006100 PE=4 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.7e-260
Identity = 446/530 (84.15%), Postives = 489/530 (92.26%), Query Frame = 0

Query: 4   TSITLPCLQPQIRFP-SQKTPARARSNVRLASVRPICAAE--KASAASPVQAPQRTVSPP 63
           +SI +P LQP +RFP SQ+TP R+RS V   S+RPI AA+   +S++S +Q PQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYAADGVSSSSSSSLQVPQRIVSPP 61

Query: 64  ERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           E TKLPLRK+PGDYGPP+ G L+DR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 SSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEKL 183
           +SD RVVVLLDGKSFPVLFD SKVEKKDLFTGTYMP TELTGGYRVLSY+DPSEP+H KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLFG 243
           KQL+FFLLK+ RDKI+PEFHSTFSELFETLEK++A AGRA YNA GEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYKRLYD 303
           A+P D+KLGRDAPKLI+KWVLFQLGPVL+LGLPK VEELLLRTVRLPPALIKADY+RLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHTR 363
           FFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG NLHTR
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDAA 423
           LAREIR+AV+A+GGKITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+ESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSPSVKN 483
           FEIKEGE+ICGYQPFATRDPKIFDR +EFVPDRF G+GEELLKHV+WSNGPETQSPSV+N
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           KQCAGKDF+VF SRLLVVELFLRYDSFDIE   +PLGAAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of MS016791.1 vs. ExPASy TrEMBL
Match: A0A6J1K6Z3 (allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491740 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.5e-260
Identity = 447/536 (83.40%), Postives = 487/536 (90.86%), Query Frame = 0

Query: 1   MSATSITLPCLQPQIRFPSQKTPARARSNVRLASVRPICAAEKASAA------SPVQAPQ 60
           MS TSITLPC QP +RFPSQK P R+RSN+   S R I AA+  S++      S VQ PQ
Sbjct: 1   MSVTSITLPCPQPHLRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQ 60

Query: 61  RTVSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           R    PE TKLPLRKIPGDYGPPV G L+DRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 61  RIAPTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120

Query: 121 PPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSE 180
           PPGPFI++D RVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSY+DPSE
Sbjct: 121 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 180

Query: 181 PNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFL 240
           P+HEKLKQL+FFLLK+ RDKILPEFHSTFSELF+TLEK++A  GRA YNA GEQAAFNFL
Sbjct: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 240

Query: 241 ARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKAD 300
           ARSLFGANP DTKLGRDAPKLI+KW+LFQLGPVL LGLPK VEELLLRTVRLPP+LIKAD
Sbjct: 241 ARSLFGANPVDTKLGRDAPKLIAKWILFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 300

Query: 301 YKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAG 360
           Y+RLYDFFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG
Sbjct: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360

Query: 361 ANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVI 420
           ANLHT+LAREIR+AV+A+GG+ITM AMEQMPLMKSVV+EAFR+EPPVP QYGRAK+D+V+
Sbjct: 361 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVHEAFRIEPPVPVQYGRAKKDLVV 420

Query: 421 ESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGEMICG+QPFATRDPKIFDR +EFVPDRF GDGE LLKHVLWSNGPETQ
Sbjct: 421 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQ 480

Query: 481 SPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           SP+V+NKQCAGKDF+VF SRLLVVE FLRYDSFDIEV  SPLGAA+TVTSLKKASF
Sbjct: 481 SPTVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of MS016791.1 vs. ExPASy TrEMBL
Match: A0A6J1HCI8 (allene oxide synthase 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462830 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.5e-260
Identity = 448/536 (83.58%), Postives = 485/536 (90.49%), Query Frame = 0

Query: 1   MSATSITLPCLQPQIRFPSQKTPARARSNVRLASVRPICAAEKASAA------SPVQAPQ 60
           MS TSITLPC QP  RFPSQK P R+RSN+   S R I AA+  S++      S VQ PQ
Sbjct: 1   MSVTSITLPCPQPHSRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQ 60

Query: 61  RTVSPPERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           R    PE TKLPLRKIPGDYGPP+ G L+DRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 61  RIAPTPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120

Query: 121 PPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSE 180
           PPGPFI++D RVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSY+DPSE
Sbjct: 121 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 180

Query: 181 PNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFL 240
           P+HEKLKQL+FFLLK+ RDKILPEFHSTFSELF+TLEK++A  GRA YNA GEQAAFNFL
Sbjct: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 240

Query: 241 ARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKAD 300
           ARSLFGANP DTKLGRDAPKLI+KWVLFQLGPVL LGLPK VEELLLRTVRLPP+LIKAD
Sbjct: 241 ARSLFGANPVDTKLGRDAPKLIAKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 300

Query: 301 YKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAG 360
           Y+RLYDFFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG
Sbjct: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360

Query: 361 ANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVI 420
           ANLHT+LAREIR+AV+A+GG+ITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+
Sbjct: 361 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420

Query: 421 ESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGEMICG+QPFATRDPKIFDR NEFVPDRF GDGE LLKHVLWSNGPETQ
Sbjct: 421 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRANEFVPDRFTGDGERLLKHVLWSNGPETQ 480

Query: 481 SPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           SP+V+NKQCAGKDF+VF SRL VVE FLRYDSFDIEV  SPLGAA+TVTSLKKASF
Sbjct: 481 SPTVQNKQCAGKDFIVFISRLFVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of MS016791.1 vs. ExPASy TrEMBL
Match: A0A1S3BAD5 (allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.3e-259
Identity = 444/530 (83.77%), Postives = 487/530 (91.89%), Query Frame = 0

Query: 4   TSITLPCLQPQIRFP-SQKTPARARSNVRLASVRPICAAE--KASAASPVQAPQRTVSPP 63
           +SI +P LQP +RFP SQ+TP R+RS V   S+RPI A +   +S++S +Q PQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSPP 61

Query: 64  ERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           E TKLPLRK+PGDYGPP+ G L+DR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 SSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEKL 183
           +SD RVVVLLDGKSFPVLFD SKVEKKDLFTGTYMP TELTGGYRVLSY+DPSEP+H KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLFG 243
           KQL+FFLLK+ RDKI+PEFHSTFSELFETLEK++A AGRA YNA GEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYKRLYD 303
           A+P D+KLGRDAPKLI+KWVLFQLGPVL+LGLPK VEELLLRTVRLPPALIKADY+RLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHTR 363
           FFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG NLHTR
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDAA 423
           LAREIR+AV+A+GGKITM AMEQMPLMKSVVYEA R+EPPVP QYGRAK+D+V+ESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSPSVKN 483
           FEIKEGE+ICGYQPFATRDPKIFDR +EFVPDRF G+GEELLKHV+WSNGPETQSPSV+N
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           KQCAGKDF+VF SRLLVVELFLRYDSFDIE   +PLGAAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of MS016791.1 vs. ExPASy TrEMBL
Match: U5YTX9 (Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.1e-258
Identity = 445/531 (83.80%), Postives = 487/531 (91.71%), Query Frame = 0

Query: 4   TSITLPCLQPQIRFP-SQKTPARARSNVRLASVRPICAAE---KASAASPVQAPQRTVSP 63
           +SI +P LQP +RFP SQ+TP R+RS V   S+RPI  A     +S++S ++ PQR VSP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVSP 61

Query: 64  PERTKLPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 123
           PE  KLPLRKIPGDYGPP+ G L+DRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 62  PEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 121

Query: 124 ISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEK 183
           I+SD RVVVLLDGKSFPVLFD SKVEKKDLFTGTYMP TELTGGYRVLSY+DPSEP+H K
Sbjct: 122 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 181

Query: 184 LKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLF 243
           LKQLVFFLLK+ RDKI+PEFHSTFSELFETLEK++A +GRA YNA GEQAAFNFLARSLF
Sbjct: 182 LKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSLF 241

Query: 244 GANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVRLPPALIKADYKRLY 303
           GA+P D+KLGRDAPKLI+KWVLFQLGPVL+LGLPK VEELLLRTVRLPPALIKADY+RLY
Sbjct: 242 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 301

Query: 304 DFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHT 363
           +FFY+SSEAVF EADRLGISREEACHNL+FTTCFNSFGGMKI FPNMIKWIGRAG NLHT
Sbjct: 302 EFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 361

Query: 364 RLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDA 423
           +LAREIR+AV+A+GGKITM AMEQMPLMKSVVYEAFR+EPPVP QYGRAK+D+V+ESHDA
Sbjct: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 421

Query: 424 AFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIGDGEELLKHVLWSNGPETQSPSVK 483
           AFEIKEGEMICGYQPFATRDPKIFDR +EFVPDRF GDGEELLKHVLWSNGPETQSPSV+
Sbjct: 422 AFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 481

Query: 484 NKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTSLKKASF 531
           NKQCAGKDF+VF SRL+VVELFLRYDSFDIE   +PLGAAVTVTSLKKASF
Sbjct: 482 NKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

BLAST of MS016791.1 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 682.9 bits (1761), Expect = 2.0e-196
Identity = 325/486 (66.87%), Postives = 404/486 (83.13%), Query Frame = 0

Query: 47  ASPVQAPQRTVSPPERTK-LPLRKIPGDYGPPVVGPLRDRQDYFYNQGREEYLKSRMLRY 106
           AS  + P  TV+    +K LP+R IPG+YG P+VGP++DR DYFY+QG EE+ KSR+ +Y
Sbjct: 33  ASGSETPDLTVATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKY 92

Query: 107 ESTVYRTNMPPGPFISSDPRVVVLLDGKSFPVLFDDSKVEKKDLFTGTYMPSTELTGGYR 166
            STVYR NMPPG FI+ +P+VV LLDGKSFPVLFD  KVEKKDLFTGTYMPSTELTGGYR
Sbjct: 93  NSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYR 152

Query: 167 VLSYLDPSEPNHEKLKQLVFFLLKNSRDKILPEFHSTFSELFETLEKEVATAGRAGYNAH 226
           +LSYLDPSEP HEKLK L+FFLLK+SR++I PEF +T+SELF++LEKE++  G+A +   
Sbjct: 153 ILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGS 212

Query: 227 GEQAAFNFLARSLFGANPADTKLGRDAPKLISKWVLFQLGPVLTLGLPKPVEELLLRTVR 286
            +  AFNFLAR+ +G NPADTKL  DAP LI+KWVLF L P+L++GLP+ +EE L+ T  
Sbjct: 213 SDGTAFNFLARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFS 272

Query: 287 LPPALIKADYKRLYDFFYESSEAVFAEADRLGISREEACHNLIFTTCFNSFGGMKILFPN 346
           LPPAL+K+DY+RLY+FF ES+  +  EAD+LGISREEA HNL+F TCFN++GGMKILFPN
Sbjct: 273 LPPALVKSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPN 332

Query: 347 MIKWIGRAGANLHTRLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQY 406
           M+K IGRAG  +H RLA EIRS ++++GG++TM A+E+M L KSVVYE  R EPPV  QY
Sbjct: 333 MVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQY 392

Query: 407 GRAKRDIVIESHDAAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFIG-DGEELLKH 466
           GRAK+D+VIESHDAAF++K GEM+ GYQP ATRDPKIFDR +EFVP+RF+G +GE+LL+H
Sbjct: 393 GRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRH 452

Query: 467 VLWSNGPETQSPSVKNKQCAGKDFVVFASRLLVVELFLRYDSFDIEVGASPLGAAVTVTS 526
           VLWSNGPET++P+V NKQCAGKDFVV  +RL V+E+F RYDSFDIEVG SPLG++V  +S
Sbjct: 453 VLWSNGPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSS 512

Query: 527 LKKASF 531
           L+KASF
Sbjct: 513 LRKASF 518

BLAST of MS016791.1 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 294.3 bits (752), Expect = 2.0e-79
Identity = 152/374 (40.64%), Postives = 221/374 (59.09%), Query Frame = 0

Query: 144 VEKKDLFTGTYMPSTELTGGYRVLSYLDPSEPNHEKLKQLVFFLLKNSRDKILPEFHSTF 203
           V+K+D+  G + PS    GG RV  YLD +EP H K+K      LK S    L E  S  
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 204 SELFETLEKEVATAGRAGYNAHGEQAAFNFLARSLFGANPA-DTKLGRDAPKLISKWVLF 263
           +  + T+E E++  G A Y    ++  F+FL  SL G + +    +  +  K I+ W+  
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLAL 123

Query: 264 QLGPVLTLG-LPKPVEELLLRTVRLPPALIKADYKRLYDFFYESS-EAVFAEADRLGISR 323
           Q+ P   LG +P+P+EE+LL T   P  LI  +YK+LY+F  E++ + +    +  G++R
Sbjct: 124 QVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFGLTR 183

Query: 324 EEACHNLIFTTCFNSFGGMKILFPNMIKWIGRAGANLHTRLAREIRSAVEAHGGKITMAA 383
           +EA  NL+F   FN++GG  +  P++I  I    + L  R+  E+R  V   G  +    
Sbjct: 184 DEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVR-RVCGSGSDLNFKT 243

Query: 384 MEQMPLMKSVVYEAFRMEPPVPFQYGRAKRDIVIESHDAAFEIKEGEMICGYQPFATRDP 443
           + +M L+KSVVYE  R  PPVP Q+ RA++D  I SHDA FE+K+GE++CGYQP   RD 
Sbjct: 244 VNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDA 303

Query: 444 KIFDRPNEFVPDRFIGD-GEELLKHVLWSNGPETQSPSVKNKQCAGKDFVVFASRLLVVE 503
            +FD P EF PDR++G+ G ELL ++ WSNGP+T +PS  NKQCA KD V   + LLV +
Sbjct: 304 NVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVAD 363

Query: 504 LFLRYDSFDIEVGA 514
           LFLRYD+   + G+
Sbjct: 364 LFLRYDTITGDSGS 376

BLAST of MS016791.1 vs. TAIR 10
Match: AT3G53280.1 (cytochrome p450 71b5 )

HSP 1 Score: 58.2 bits (139), Expect = 2.4e-08
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 0

Query: 360 RLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGR-AKRDIVIESHD 419
           +L  EIR+A+  +  KIT   +E++  +K V+ EAFR+ PP P    R    DI I+   
Sbjct: 324 KLQEEIRAALGPNKEKITEEDLEKVEYLKMVIEEAFRLHPPAPLLLPRLTMSDINIQ--- 383

Query: 420 AAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFI 456
             + I +  MI        RDPK + +P+EF+P+RF+
Sbjct: 384 -GYSIPKNTMIQINTYTIGRDPKNWTKPDEFIPERFV 416

BLAST of MS016791.1 vs. TAIR 10
Match: AT3G26190.1 (cytochrome P450, family 71, subfamily B, polypeptide 21 )

HSP 1 Score: 53.5 bits (127), Expect = 5.9e-07
Identity = 32/97 (32.99%), Postives = 50/97 (51.55%), Query Frame = 0

Query: 360 RLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGR-AKRDIVIESHD 419
           +L +EIR  +  +  KIT   +E++  +K V+ E FR+ PP P    R    D+ I+   
Sbjct: 326 KLQQEIRELLGDNKEKITEQDLEKVHYLKLVIQETFRLHPPAPLLLPRETMSDVKIQ--- 385

Query: 420 AAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFI 456
             + I +  MI        RDP  +  PNEF+P+RF+
Sbjct: 386 -GYNIPKNTMIEINTYAIGRDPNCWTNPNEFIPERFV 418

BLAST of MS016791.1 vs. TAIR 10
Match: AT3G44250.1 (cytochrome P450, family 71, subfamily B, polypeptide 38 )

HSP 1 Score: 52.8 bits (125), Expect = 1.0e-06
Identity = 32/97 (32.99%), Postives = 51/97 (52.58%), Query Frame = 0

Query: 360 RLAREIRSAVEAHGGKITMAAMEQMPLMKSVVYEAFRMEPPVPFQYGR-AKRDIVIESHD 419
           +L  EIR+ +  +  +IT   +E++  +K V+ E FR+ PP P    R    DI I+   
Sbjct: 325 KLQEEIRAMLGPNKERITEEDLEKVEYLKLVMVETFRLHPPAPLLLPRLTMSDIKIQ--- 384

Query: 420 AAFEIKEGEMICGYQPFATRDPKIFDRPNEFVPDRFI 456
             + I +  MI        RDPK + +P EF+P+RF+
Sbjct: 385 -GYNIPKNTMIQINTYAIGRDPKYWKQPGEFIPERFL 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885277.17.4e-26284.46allene oxide synthase 1, chloroplastic [Benincasa hispida][more]
KAG7029361.17.0e-26083.77Allene oxide synthase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. a... [more]
XP_023547133.19.1e-26083.77allene oxide synthase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAA0065268.11.2e-25984.15allene oxide synthase 1 [Cucumis melo var. makuwa][more]
XP_022962362.11.5e-25983.58allene oxide synthase 1, chloroplastic-like [Cucurbita moschata] >KAG6598409.1 A... [more]
Match NameE-valueIdentityDescription
K4BV521.4e-21568.28Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
P484173.1e-20264.34Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE... [more]
Q9LLB03.8e-20068.57Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
Q962422.8e-19566.87Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
Q407785.8e-18565.24Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7VCB05.7e-26084.15Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1K6Z37.5e-26083.40allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HCI87.5e-26083.58allene oxide synthase 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A1S3BAD58.3e-25983.77allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 P... [more]
U5YTX94.1e-25883.80Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G42650.12.0e-19666.87allene oxide synthase [more]
AT4G15440.12.0e-7940.64hydroperoxide lyase 1 [more]
AT3G53280.12.4e-0836.08cytochrome p450 71b5 [more]
AT3G26190.15.9e-0732.99cytochrome P450, family 71, subfamily B, polypeptide 21 [more]
AT3G44250.11.0e-0632.99cytochrome P450, family 71, subfamily B, polypeptide 38 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 437..455
score: 35.22
coord: 384..400
score: 31.67
coord: 483..501
score: 21.66
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 61..530
e-value: 5.1E-122
score: 410.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 66..506
IPR001128Cytochrome P450PFAMPF00067p450coord: 359..455
e-value: 1.8E-15
score: 56.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..76
NoneNo IPR availablePANTHERPTHR24286:SF267ALLENE OXIDE SYNTHASE 1, CHLOROPLASTIC-LIKEcoord: 47..528
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 47..528

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS016791MS016791gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS016791.1-cdsMS016791.1-cds-scaffold9_1:51105..52694CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS016791.1MS016791.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen