MS016228.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS016228.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	phosphatidyl-N-methylethanolamine N-methyltransferase
Location	scaffold9_2: 1242869 .. 1245476 (+)
Sequence length	393
RNA-Seq Expression	MS016228.1
Synteny	MS016228.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCATAATCGCAGGAATTGGAGTGCTGCTGCCATTTCCCTTCTACTACGTCTTATGGACTCGTCCACAGTTATGGGCGAATTTTTGCGGGAAATGGCGCGAACCCTGCAAATTGATGGCTCGGGTCTCCATTTTTCTCAAGTTGCTTCAGTTCCTCTCCATCCTCCACGTCTCCTCCTTCTCATGGCCGCCGCCTCTCTACTTCTGGCCCCTCATGGCCTTTGGCCAATTTTTGAACTTCAGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGATGTGCTGAAATCTGCCTTCAATCTGTATAGTAGCCTACTTGACTTAACTTTTGTGCTACTGCTTGCTATATTGATGAGAGGAAATTTACGAGCTGGATATGTGGAAGTAACGATTTATTAATGATCTCTGTTCACTAAATGACTAAACCATCTGAAAAGCGATCCTACCTGTGAATTTAATGATTAAATTTCTCTTTACTTCTTAAGATTACTGGATTAGGAGATTATTAGCATTTAATAAAGGTTAGAATCATTGGCAATCTCTTCTTTATCAGGGAGAGAGAGAGACGAAATTTGATTTCAGTTTCTATCTTCAAGTTTAGCTATCGCTGATCGTGATCAATCTCTTCATTCTCCAATTGACCATTCAGTTGATGTATATGTGGAAGGTTTTTTTTTCCCTGTACTTTTTGGGTAAGAAATAGTTGTTGCACCAAAGTAGGTCCTAGAAAGTGAAATACTTGGAGTTTGGTCTTCGCTACAAGGTTTTATACATCAAGCGGTCGTGGCTCTGAAGTTCTTTTGGTTTTTCAAGCTTAGTAGGAAGCAGTTTTCTGAGACCTGGTTTAGAATGCTTTTTTATCTTTTTTGAGTTTGTACTCTTGTTGTGCTAATTCTGCTTTATTTTTCTCTTCTTCTCTCTCTTATTTTTTCACCCTTTTGGAGTCCTATATCCTGAACATTTTTCCTTTTCGATGAAAAATTTTTTTTTTCCAAAAGAAAGGAAGTGAATACTTGGTGTGACCGACTTATCTGAGGCTGCTGCAGTGTCCCGAGTACTGTTTTTCATTTCTATTAAGGATTACTGTATTGTGCTTCCCAGTGCTCACATCCTTGTTATCACTTTTATAAGAATTCAACTTGGTTCTGTCTTTCCCAAAGAATGCTTCCCCTCTAGCCTTGAACAACTTTATAAAGTTACCTTTGAGAAAAGTGGTGTCGAGGGGATTTTCCTGAAATTTGACTTCGTAGAAGTTACAGACTTTTCAAGAATACTTCTCGAGGAAGTGAAAAAAAACTTAGAAGTTGTGCGTTTTATAGTACTTCTATAAAGTTTCCTATCTTCAAAATCTTTTTGTAACTATGTACATATTTTTTTATTAATGCTATTCTAGTGGACTGTACTTAGTCTGCTAGTTTCGTTTAAGTTGATGCCTCAACCACCCAATTTATTTCTCTTGGTGTTAATGGAAGTTTGTTTCCTATTAAAAAGAAGAAAGAAATTGATGTGGATAGGTTTATATCAAGATTTTCCCCTATCATAATGCAACAGTAAAAAGTGAAAGTGTGGAGATTGTTGCGCCAAAGTGAGCACATAAATTTAGAAATCAATAGTTGCAAGGGCCATTTCCTTGCCTCGGATTCTGTGTACTTTAATGAGATGAGAATGATTGCAATCTAAGGAATTGGCTTTATCTGCTCTTGGGTTGAGGAATGGAATTTTTAATATGAATAAGAATCTATCATAGATGTCCTTTCGTTGGTTTGCAATATCCAAGCAATTACTAAAATATATGTTCAAATTCATTATGCTGAACTTTATCTGCTCTTGGATACTCTTTCATTGATTTCAGAGATGAAAAATGATGCTTGCAATGGTCAAACAAGCTTTAGAGACAGCATTTTCCCAGTATAATTGCCTCATATGATTGGTAGTCTTCACAAGATAGATCTTTTGTGATTATTACTATATAACAAAGTAAAAACAACTAAGATTACATTAATGTTCGTTTCTTATGTTCAAGCTTGAAAATTCAGAAACAAGCGTAGAATAAGCATCTCAGGCTGCTAGTGTTTTAATTAAAAGCAAAGAAACTATAATCAAGATTTTCGAGTTTAGGATGTTCTATCCTAAATTTTTAGGCCAATTTAAGGATACATAAGTGATCTCATGGGACAACTGTCCCTGTCTAACCTAGTAGGGTAGATTTTAATATCTTAAGTTGGTGGCCACATGAGTTTCAACCCATTCTCTCAAAGTCTATATATTTTTTTGACCCACCTCTATAACCATCAGGCCAATCCGGACAGGGTCAAGTACGATTAGATGCATGAGACTATTGGACCTCAATTTTTTACAATGTTGCTGCAGTTTTCCAGACTCGATTGAATGAAAACAATATCGTATTGCTCCGGCTTGTATTATAAAGTAATTCTTTCTTCTGTCTTCTGCCAAATTATAGGGTCTATCAGTTACTGGGTGAATCCGGTGTTTACTATGGAGTACGGTTTGGGAAGAACATTCCATGGGTGACAGAATTTCCATTTGGATATATAAGAGATCCTCAGTATGTGGGAAGCATTTTGAGCATTTTTGCTTGTCTTTCTTGG

mRNA sequence

ATGGGCATAATCGCAGGAATTGGAGTGCTGCTGCCATTTCCCTTCTACTACGTCTTATGGACTCGTCCACAGTTATGGGCGAATTTTTGCGGGAAATGGCGCGAACCCTGCAAATTGATGGCTCGGGTCTCCATTTTTCTCAAGTTGCTTCAGTTCCTCTCCATCCTCCACGTCTCCTCCTTCTCATGGCCGCCGCCTCTCTACTTCTGGCCCCTCATGGCCTTTGGCCAATTTTTGAACTTCAGGGTCTATCAGTTACTGGGTGAATCCGGTGTTTACTATGGAGTACGGTTTGGGAAGAACATTCCATGGGTGACAGAATTTCCATTTGGATATATAAGAGATCCTCAGTATGTGGGAAGCATTTTGAGCATTTTTGCTTGTCTTTCTTGG

Coding sequence (CDS)

ATGGGCATAATCGCAGGAATTGGAGTGCTGCTGCCATTTCCCTTCTACTACGTCTTATGGACTCGTCCACAGTTATGGGCGAATTTTTGCGGGAAATGGCGCGAACCCTGCAAATTGATGGCTCGGGTCTCCATTTTTCTCAAGTTGCTTCAGTTCCTCTCCATCCTCCACGTCTCCTCCTTCTCATGGCCGCCGCCTCTCTACTTCTGGCCCCTCATGGCCTTTGGCCAATTTTTGAACTTCAGGGTCTATCAGTTACTGGGTGAATCCGGTGTTTACTATGGAGTACGGTTTGGGAAGAACATTCCATGGGTGACAGAATTTCCATTTGGATATATAAGAGATCCTCAGTATGTGGGAAGCATTTTGAGCATTTTTGCTTGTCTTTCTTGG

Protein sequence

MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVGSILSIFACLSW

Homology

[Close]

BLAST of MS016228.1 vs. NCBI nr
Match: XP_022153398.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Momordica charantia])

HSP 1 Score: 285.0 bits (728), Expect = 3.2e-73
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS
Sbjct: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILSIFACLSW
Sbjct: 121 SILSIFACLSW 131

[Close]

BLAST of MS016228.1 vs. NCBI nr
Match: XP_008465151.2 (PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo])

HSP 1 Score: 263.8 bits (673), Expect = 7.6e-67
Identity = 119/131 (90.84%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MGI+A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSS
Sbjct: 1   MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+FACLSW
Sbjct: 121 SILSLFACLSW 131

[Close]

BLAST of MS016228.1 vs. NCBI nr
Match: XP_004137657.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus] >KAE8650956.1 hypothetical protein Csa_002414 [Cucumis sativus])

HSP 1 Score: 263.1 bits (671), Expect = 1.3e-66
Identity = 119/131 (90.84%), Postives = 125/131 (95.42%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MGI A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSS
Sbjct: 1   MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+FACLSW
Sbjct: 121 SILSLFACLSW 131

[Close]

BLAST of MS016228.1 vs. NCBI nr
Match: XP_038903463.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Benincasa hispida])

HSP 1 Score: 259.2 bits (661), Expect = 1.9e-65
Identity = 115/131 (87.79%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MGI+A +GVLLPFP+YYVLW+RPQLW N CGK R+PCKLMA+VS+FLKLLQFLSI HVSS
Sbjct: 1   MGILAVLGVLLPFPYYYVLWSRPQLWVNLCGKSRDPCKLMAQVSVFLKLLQFLSIFHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           F+WPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FAWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+FACLSW
Sbjct: 121 SILSLFACLSW 131

[Close]

BLAST of MS016228.1 vs. NCBI nr
Match: KAG7036543.1 (Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258.5 bits (659), Expect = 3.2e-65
Identity = 116/131 (88.55%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+ ACLSW
Sbjct: 121 SILSLLACLSW 131

[Close]

BLAST of MS016228.1 vs. ExPASy Swiss-Prot
Match: Q9SAH5 (Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=PLMT PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.7e-56
Identity = 99/131 (75.57%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
            SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGKNIPWVTEFPFG IRDPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 121 SILSIFACLSW 132
           SI+S+ ACLSW
Sbjct: 121 SIMSLLACLSW 131

[Close]

BLAST of MS016228.1 vs. ExPASy TrEMBL
Match: A0A6J1DHC8 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020909 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.5e-73
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS
Sbjct: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILSIFACLSW
Sbjct: 121 SILSIFACLSW 131

[Close]

BLAST of MS016228.1 vs. ExPASy TrEMBL
Match: A0A1S3CPQ5 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103502819 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.7e-67
Identity = 119/131 (90.84%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MGI+A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSS
Sbjct: 1   MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+FACLSW
Sbjct: 121 SILSLFACLSW 131

[Close]

BLAST of MS016228.1 vs. ExPASy TrEMBL
Match: A0A0A0LCJ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710860 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.3e-67
Identity = 119/131 (90.84%), Postives = 125/131 (95.42%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MGI A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSS
Sbjct: 1   MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+FACLSW
Sbjct: 121 SILSLFACLSW 131

[Close]

BLAST of MS016228.1 vs. ExPASy TrEMBL
Match: A0A6J1KB80 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111492722 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.5e-65
Identity = 115/131 (87.79%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           M ++A IGVLLPFP+YY+LWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYLLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+ ACLSW
Sbjct: 121 SILSLLACLSW 131

[Close]

BLAST of MS016228.1 vs. ExPASy TrEMBL
Match: A0A6J1GCG7 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111452701 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 7.7e-65
Identity = 115/131 (87.79%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQ LSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQILSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSIFACLSW 132
           SILS+ ACLSW
Sbjct: 121 SILSLLACLSW 131

[Close]

BLAST of MS016228.1 vs. TAIR 10
Match: AT1G80860.2 (phospholipid N-methyltransferase )

HSP 1 Score: 219.9 bits (559), Expect = 1.2e-57
Identity = 99/131 (75.57%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
            SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGKNIPWVTEFPFG IRDPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 121 SILSIFACLSW 132
           SI+S+ ACLSW
Sbjct: 121 SIMSLLACLSW 131

[Close]

BLAST of MS016228.1 vs. TAIR 10
Match: AT1G80860.1 (phospholipid N-methyltransferase )

HSP 1 Score: 219.9 bits (559), Expect = 1.2e-57
Identity = 99/131 (75.57%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60
           MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120
            SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGKNIPWVTEFPFG IRDPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 121 SILSIFACLSW 132
           SI+S+ ACLSW
Sbjct: 121 SIMSLLACLSW 131

The following BLAST results are available for this feature:

BLAST of MS016228.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153398.1	3.2e-73	100.00	phosphatidyl-N-methylethanolamine N-methyltransferase [Momordica charantia]	[more]
XP_008465151.2	7.6e-67	90.84	PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo]	[more]
XP_004137657.1	1.3e-66	90.84	phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus] >KAE8650...	[more]
XP_038903463.1	1.9e-65	87.79	phosphatidyl-N-methylethanolamine N-methyltransferase [Benincasa hispida]	[more]
KAG7036543.1	3.2e-65	88.55	Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma su...	[more]

BLAST of MS016228.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAH5	1.7e-56	75.57	Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana OX...	[more]

BLAST of MS016228.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHC8	1.5e-73	100.00	phosphatidyl-N-methylethanolamine N-methyltransferase OS=Momordica charantia OX=...	[more]
A0A1S3CPQ5	3.7e-67	90.84	phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucumis melo OX=3656 GN...	[more]
A0A0A0LCJ2	6.3e-67	90.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710860 PE=4 SV=1	[more]
A0A6J1KB80	3.5e-65	87.79	phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita maxima OX=366...	[more]
A0A6J1GCG7	7.7e-65	87.79	phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita moschata OX=3...	[more]

BLAST of MS016228.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80860.2	1.2e-57	75.57	phospholipid N-methyltransferase	[more]
AT1G80860.1	1.2e-57	75.57	phospholipid N-methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 15..131 e-value: 1.4E-5 score: 26.9
None	No IPR available	PANTHER	PTHR15458:SF5	PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE	coord: 10..131
IPR007318	Phospholipid methyltransferase	PFAM	PF04191	PEMT	coord: 71..127 e-value: 6.0E-10 score: 39.5
IPR024960	Phosphatidyl-N-methylethanolamine/Phosphatidylethanolamine N-methyltransferase	PANTHER	PTHR15458	PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE	coord: 10..131

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS016228	MS016228	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS016228.1-cds	MS016228.1-cds-scaffold9_2:1242869..1243113	CDS
MS016228.1-cds	MS016228.1-cds-scaffold9_2:1245329..1245476	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS016228.1	MS016228.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006656	phosphatidylcholine biosynthetic process
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity