MS016224.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS016224.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Phytocyanin domain-containing protein
Location	scaffold9_2: 1189155 .. 1189799 (-)
Sequence length	507
RNA-Seq Expression	MS016224.1
Synteny	MS016224.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTCGCACTCGCCGCACCTTGCATTCTCCTTCCTCATCCTCGCCGCCGCCGCCGCCGTCGTCTCCGCCACCGACCACATCGTCGGCGCCAACCGCGGCTGGAACCCTGGCATCAACTACACTCTCTGGGCCAACAACCACACCTTCTTCGTCGGCGACTTCATCTGTAATTTTCTCTCTCTTTCTCCCTAATAGCTAGGGTTTCACGGGAGAGACTACAATTTAAGTTCGTGTATAATCTTTTTTATCCTAGAAATGTTGAATCTAAGATGAACTAAGGGGGAAATGGCCCTATATTTGGCAGCATTTAGGTATCAGAAGATCCAATACAATGTGTTCGAGGTGAACCAATCAGGGTACGGCAACTGCACCACAGAGGGGGCGTTTGGGAATTGGAGCAGTGGCAAAGACTTCATCCTTCTCAAGGAGGCCAAAAGATACTACTTCATTTGTGGCAATGGGCAGTGCTACAATGGCATGAAGGTCACCATTCTTGTCCATCCTCTTCCTCCTCCTCCATCTTCTCCCCTTGCCGCCAAGCATTCCTCGCCCTCGTCTGCCCCGGCCCGGTTGGGTTCTTGGCCGACACGGATTCAGGCTCTGCTCGTTTCTCTGGCTTTGATCTGGTTTGGATCTGAATGGATT

mRNA sequence

GCCTCGCACTCGCCGCACCTTGCATTCTCCTTCCTCATCCTCGCCGCCGCCGCCGCCGTCGTCTCCGCCACCGACCACATCGTCGGCGCCAACCGCGGCTGGAACCCTGGCATCAACTACACTCTCTGGGCCAACAACCACACCTTCTTCGTCGGCGACTTCATCTCATTTAGGTATCAGAAGATCCAATACAATGTGTTCGAGGTGAACCAATCAGGGTACGGCAACTGCACCACAGAGGGGGCGTTTGGGAATTGGAGCAGTGGCAAAGACTTCATCCTTCTCAAGGAGGCCAAAAGATACTACTTCATTTGTGGCAATGGGCAGTGCTACAATGGCATGAAGGTCACCATTCTTGTCCATCCTCTTCCTCCTCCTCCATCTTCTCCCCTTGCCGCCAAGCATTCCTCGCCCTCGTCTGCCCCGGCCCGGTTGGGTTCTTGGCCGACACGGATTCAGGCTCTGCTCGTTTCTCTGGCTTTGATCTGGTTTGGATCTGAATGGATT

Coding sequence (CDS)

GCCTCGCACTCGCCGCACCTTGCATTCTCCTTCCTCATCCTCGCCGCCGCCGCCGCCGTCGTCTCCGCCACCGACCACATCGTCGGCGCCAACCGCGGCTGGAACCCTGGCATCAACTACACTCTCTGGGCCAACAACCACACCTTCTTCGTCGGCGACTTCATCTCATTTAGGTATCAGAAGATCCAATACAATGTGTTCGAGGTGAACCAATCAGGGTACGGCAACTGCACCACAGAGGGGGCGTTTGGGAATTGGAGCAGTGGCAAAGACTTCATCCTTCTCAAGGAGGCCAAAAGATACTACTTCATTTGTGGCAATGGGCAGTGCTACAATGGCATGAAGGTCACCATTCTTGTCCATCCTCTTCCTCCTCCTCCATCTTCTCCCCTTGCCGCCAAGCATTCCTCGCCCTCGTCTGCCCCGGCCCGGTTGGGTTCTTGGCCGACACGGATTCAGGCTCTGCTCGTTTCTCTGGCTTTGATCTGGTTTGGATCTGAATGGATT

Protein sequence

ASHSPHLAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVHPLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI

Homology

[Close]

BLAST of MS016224.1 vs. NCBI nr
Match: XP_022153463.1 (lamin-like protein [Momordica charantia])

HSP 1 Score: 352.1 bits (902), Expect = 2.8e-93
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 1   ASHSPHLAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQ 60
           ASHSPHLAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQ
Sbjct: 3   ASHSPHLAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQ 62

Query: 61  KIQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILV 120
           KIQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILV
Sbjct: 63  KIQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILV 122

Query: 121 HPLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           HPLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI
Sbjct: 123 HPLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 171

[Close]

BLAST of MS016224.1 vs. NCBI nr
Match: XP_023526524.1 (lamin-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.0 bits (728), Expect = 4.1e-73
Identity = 140/168 (83.33%), Postives = 149/168 (88.69%), Query Frame = 0


Query: 4   SPHLAFSFLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQK 63
           SPHL   FL+L  AA+V  VSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFRYQK
Sbjct: 4   SPHLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFRYQK 63

Query: 64  IQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH 123
            QYNVFEVNQSGY NCTTEGAFGNWSSGKDFI L +A+RYYFICGNGQC+NGMKVTILVH
Sbjct: 64  NQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTILVH 123

Query: 124 PLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           PLPPPPSSPLA+K SSPSSA  R GS   +I+ALLVSLALIWFGS+WI
Sbjct: 124 PLPPPPSSPLASKDSSPSSATPRTGSRQWQIRALLVSLALIWFGSDWI 171

[Close]

BLAST of MS016224.1 vs. NCBI nr
Match: KAG7018121.1 (Lamin-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282.3 bits (721), Expect = 2.7e-72
Identity = 139/168 (82.74%), Postives = 148/168 (88.10%), Query Frame = 0


Query: 4   SPHLAFSFLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQK 63
           SP L   FL+L  AA+V  VSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFRYQK
Sbjct: 4   SPQLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFRYQK 63

Query: 64  IQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH 123
            QYNVFEVNQSGY NCTTEGAFGNWSSGKDFI L +A+RYYFICGNGQC+NGMKVTILVH
Sbjct: 64  NQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTILVH 123

Query: 124 PLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           PLPPPPSSPLA+K SSPSSA  R GS   +I+ALLVSLALIWFGS+WI
Sbjct: 124 PLPPPPSSPLASKDSSPSSATPRTGSRQWKIRALLVSLALIWFGSDWI 171

[Close]

BLAST of MS016224.1 vs. NCBI nr
Match: XP_038903873.1 (lamin-like protein [Benincasa hispida])

HSP 1 Score: 281.6 bits (719), Expect = 4.6e-72
Identity = 136/168 (80.95%), Postives = 148/168 (88.10%), Query Frame = 0


Query: 4   SPHLAFSFLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQK 63
           SPHL   FL+L AA +V  VSATDHIVGANRGWNPGINYTLWANNHTF+VGD ISFRYQK
Sbjct: 5   SPHLLAFFLLLCAAVSVFPVSATDHIVGANRGWNPGINYTLWANNHTFYVGDLISFRYQK 64

Query: 64  IQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH 123
            QYNVFEVNQSGY NCTTEGA GNWSSGKDFI L++A+RYYFICGNGQC+NGMKVT+LVH
Sbjct: 65  NQYNVFEVNQSGYDNCTTEGALGNWSSGKDFIPLQKAQRYYFICGNGQCFNGMKVTVLVH 124

Query: 124 PLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           PLPPPPSSPL  +HSSPSSA A  GS   RI+ALL+SLALIWFGS+WI
Sbjct: 125 PLPPPPSSPLGTEHSSPSSATAPEGSQQWRIRALLISLALIWFGSDWI 172

[Close]

BLAST of MS016224.1 vs. NCBI nr
Match: XP_022934432.1 (lamin-like protein [Cucurbita moschata])

HSP 1 Score: 280.4 bits (716), Expect = 1.0e-71
Identity = 138/168 (82.14%), Postives = 147/168 (87.50%), Query Frame = 0


Query: 4   SPHLAFSFLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQK 63
           SP L   FL+L  AA+V  VSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFRYQK
Sbjct: 4   SPQLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFRYQK 63

Query: 64  IQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH 123
            QYNVFEVNQSGY NCTTEGAFGNWSSGKDFI L +A+RYYFICGNGQC+NGMKVTILVH
Sbjct: 64  NQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTILVH 123

Query: 124 PLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           PLPPPPSSPLA+K SSPSSA  R GS   +I+AL VSLALIWFGS+WI
Sbjct: 124 PLPPPPSSPLASKDSSPSSATPRTGSRQWQIRALFVSLALIWFGSDWI 171

[Close]

BLAST of MS016224.1 vs. ExPASy Swiss-Prot
Match: Q39131 (Lamin-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g15350 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.7e-24
Identity = 55/158 (34.81%), Postives = 87/158 (55.06%), Query Frame = 0


Query: 13  ILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFEVNQS 72
           ++AA    V+A  + VG N+ WNP INYT+WA    F++GD++ F + + Q+N+ EVN++
Sbjct: 16  LMAAPMPGVTAKKYTVGENKFWNPNINYTIWAQGKHFYLGDWLYFVFDRNQHNILEVNKT 75

Query: 73  GYGNCTTEGAFGNWS--SGKDFILLKEAKRYYFICGNGQCYNGMKVTILVHPLPPPPSSP 132
            Y  C  +    NW+  +G+D + L + K YY + G G CY GMK+++ V  LPPPP S 
Sbjct: 76  DYEGCIADHPIRNWTRGAGRDIVTLNQTKHYYLLDGKGGCYGGMKLSVKVEKLPPPPKSA 135

Query: 133 LAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEW 169
                 S S          T +   ++ ++L  F + W
Sbjct: 136 PVKNIGSVSMV--------TGLAQFMIPVSLFAFPAMW 165

[Close]

BLAST of MS016224.1 vs. ExPASy Swiss-Prot
Match: G7L0H3 (Blue copper protein 1a OS=Medicago truncatula OX=3880 GN=MTR_7g086140 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.1e-15
Identity = 53/161 (32.92%), Postives = 85/161 (52.80%), Query Frame = 0


Query: 7   LAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNV 66
           L+ S ++L++ A    ATDHIVG ++GW    +YT WA +  F VGD + F Y   ++NV
Sbjct: 10  LSISMVLLSSVAI---ATDHIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPARHNV 69

Query: 67  FEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNG----MKVTILVHP 126
           F+VN + + +CT        S+GKD I LK   R +++CG     +     + +T+L   
Sbjct: 70  FKVNGTLFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVLAEG 129

Query: 127 LPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIW 164
            P P   P +  HS  SS           + A++V++A+I+
Sbjct: 130 APAPSPPPSSDAHSVVSSLFG-------VVMAIMVAIAVIF 160

[Close]

BLAST of MS016224.1 vs. ExPASy Swiss-Prot
Match: A0A072U307 (Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.1e-15
Identity = 52/161 (32.30%), Postives = 87/161 (54.04%), Query Frame = 0


Query: 7   LAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNV 66
           L+ S ++L++ A  ++ATD+IVG ++GW    +YT WA +  F VGD + F Y   ++NV
Sbjct: 10  LSISMVLLSSVA--IAATDYIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPSRHNV 69

Query: 67  FEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNG----MKVTILVHP 126
           F+VN + + +CT        S+GKD I LK   R +++CG     +     + +T+L   
Sbjct: 70  FKVNGTLFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVLAEG 129

Query: 127 LPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIW 164
            P P   P +  HS  SS           + A++V++A+I+
Sbjct: 130 APAPSPPPSSDAHSVVSSLFG-------VVMAIMVAIAVIF 161

[Close]

BLAST of MS016224.1 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 51/139 (36.69%), Postives = 74/139 (53.24%), Query Frame = 0


Query: 7   LAFSFLILAAAAAVVS-ATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYN 66
           L   FL+     A+ S AT + VG   GW  G +Y+ WA++ TF VGD + F Y    + 
Sbjct: 7   LVLCFLLAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYGAGAHT 66

Query: 67  VFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICG-NGQCYNGMKVTILVHPLP 126
           V EV +S Y +CT+  +    S+G   I LK+A ++YFICG  G    GMK++I V    
Sbjct: 67  VDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKASS 126

Query: 127 PPPSSPLAAKHSSPSSAPA 144
              ++P A   SS   +P+
Sbjct: 127 GSSAAPSATPSSSGKGSPS 145

[Close]

BLAST of MS016224.1 vs. ExPASy Swiss-Prot
Match: A0A0M4FTF3 (Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-12
Identity = 35/91 (38.46%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 16  AAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFEVNQSGYG 75
           A A+    T++ VG ++GW   ++Y  WA   TF VGD + F+Y K  +NVF+VNQ+G+ 
Sbjct: 108 APASPAPQTEYWVGDDKGWTIDVDYQAWAKGKTFKVGDTLVFKYTKGHHNVFKVNQTGFQ 167

Query: 76  NCTTEGAFGNWSSGKDFILLKEAKRYYFICG 107
           NC         +SG D I L    + ++ICG
Sbjct: 168 NCIAPPPSEGLTSGHDVITLAAPGKKWYICG 198

[Close]

BLAST of MS016224.1 vs. ExPASy TrEMBL
Match: A0A6J1DHI7 (lamin-like protein OS=Momordica charantia OX=3673 GN=LOC111020966 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.3e-93
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 1   ASHSPHLAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQ 60
           ASHSPHLAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQ
Sbjct: 3   ASHSPHLAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQ 62

Query: 61  KIQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILV 120
           KIQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILV
Sbjct: 63  KIQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILV 122

Query: 121 HPLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           HPLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI
Sbjct: 123 HPLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 171

[Close]

BLAST of MS016224.1 vs. ExPASy TrEMBL
Match: A0A6J1F2R0 (lamin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441613 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.9e-72
Identity = 138/168 (82.14%), Postives = 147/168 (87.50%), Query Frame = 0


Query: 4   SPHLAFSFLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQK 63
           SP L   FL+L  AA+V  VSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFRYQK
Sbjct: 4   SPQLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFRYQK 63

Query: 64  IQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH 123
            QYNVFEVNQSGY NCTTEGAFGNWSSGKDFI L +A+RYYFICGNGQC+NGMKVTILVH
Sbjct: 64  NQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTILVH 123

Query: 124 PLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           PLPPPPSSPLA+K SSPSSA  R GS   +I+AL VSLALIWFGS+WI
Sbjct: 124 PLPPPPSSPLASKDSSPSSATPRTGSRQWQIRALFVSLALIWFGSDWI 171

[Close]

BLAST of MS016224.1 vs. ExPASy TrEMBL
Match: A0A6J1J7T1 (lamin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482049 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 8.4e-72
Identity = 136/161 (84.47%), Postives = 146/161 (90.68%), Query Frame = 0


Query: 11  FLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFE 70
           FL+L  AA+V  VSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFRYQK QYNVFE
Sbjct: 11  FLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFRYQKNQYNVFE 70

Query: 71  VNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVHPLPPPPS 130
           VNQSGY NCTTEGAFGNWSSGKDFI L +A+RYYFICGNGQC+NGMKVTILVHPLPPPPS
Sbjct: 71  VNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTILVHPLPPPPS 130

Query: 131 SPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           SPLA+K+SSPSSA  R GS   +I+ALLVSLALIWFGS+WI
Sbjct: 131 SPLASKNSSPSSATPRTGSRQWQIRALLVSLALIWFGSDWI 171

[Close]

BLAST of MS016224.1 vs. ExPASy TrEMBL
Match: A0A1S3CN34 (lamin-like protein OS=Cucumis melo OX=3656 GN=LOC103502815 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.5e-68
Identity = 131/168 (77.98%), Postives = 144/168 (85.71%), Query Frame = 0


Query: 4   SPHLAFSFLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQK 63
           SPHL   FL+L AA ++  VSATDHIVGANRGWNPGINYTLWANNHTF+VGD ISFRYQK
Sbjct: 4   SPHLLPFFLLLCAAVSLFPVSATDHIVGANRGWNPGINYTLWANNHTFYVGDLISFRYQK 63

Query: 64  IQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH 123
            QYNVFEVNQ+GY NCTTEGAFGNWSSGKDFI L +A+RYYFICGNGQC+NGMKVTILVH
Sbjct: 64  NQYNVFEVNQTGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTILVH 123

Query: 124 PLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           PLPPPPSSPLA +H SPSSA   +GS     +ALL+SLA+I FGS WI
Sbjct: 124 PLPPPPSSPLATEHHSPSSATPCVGSQQWGFRALLISLAMILFGSHWI 171

[Close]

BLAST of MS016224.1 vs. ExPASy TrEMBL
Match: A0A0A0LCI9 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G710810 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.4e-65
Identity = 127/168 (75.60%), Postives = 141/168 (83.93%), Query Frame = 0


Query: 4   SPHLAFSFLILAAAAAV--VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQK 63
           SP L   FL+L AA ++  VSATDHIVGANRGWNPGINYTLWANNHTF+VGD ISFRYQK
Sbjct: 4   SPRLLPFFLLLCAAVSLFPVSATDHIVGANRGWNPGINYTLWANNHTFYVGDLISFRYQK 63

Query: 64  IQYNVFEVNQSGYGNCTTEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH 123
            QYNVFEVNQ+GY NCT EGA GNWSSGKDFI L +A+RYYFICGNGQC+NGMKVTILVH
Sbjct: 64  NQYNVFEVNQTGYDNCTIEGASGNWSSGKDFIPLDKAQRYYFICGNGQCFNGMKVTILVH 123

Query: 124 PLPPPPSSPLAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEWI 170
           PLPPPPSSPLA +H+SPSSA   +GS     +ALL+SLA+I  GS WI
Sbjct: 124 PLPPPPSSPLAMEHNSPSSATPWVGSRQWGFRALLISLAMILLGSHWI 171

[Close]

BLAST of MS016224.1 vs. TAIR 10
Match: AT5G15350.1 (early nodulin-like protein 17 )

HSP 1 Score: 112.8 bits (281), Expect = 2.6e-25
Identity = 55/158 (34.81%), Postives = 87/158 (55.06%), Query Frame = 0


Query: 13  ILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFEVNQS 72
           ++AA    V+A  + VG N+ WNP INYT+WA    F++GD++ F + + Q+N+ EVN++
Sbjct: 16  LMAAPMPGVTAKKYTVGENKFWNPNINYTIWAQGKHFYLGDWLYFVFDRNQHNILEVNKT 75

Query: 73  GYGNCTTEGAFGNWS--SGKDFILLKEAKRYYFICGNGQCYNGMKVTILVHPLPPPPSSP 132
            Y  C  +    NW+  +G+D + L + K YY + G G CY GMK+++ V  LPPPP S 
Sbjct: 76  DYEGCIADHPIRNWTRGAGRDIVTLNQTKHYYLLDGKGGCYGGMKLSVKVEKLPPPPKSA 135

Query: 133 LAAKHSSPSSAPARLGSWPTRIQALLVSLALIWFGSEW 169
                 S S          T +   ++ ++L  F + W
Sbjct: 136 PVKNIGSVSMV--------TGLAQFMIPVSLFAFPAMW 165

[Close]

BLAST of MS016224.1 vs. TAIR 10
Match: AT3G01070.1 (early nodulin-like protein 16 )

HSP 1 Score: 110.2 bits (274), Expect = 1.7e-24
Identity = 54/126 (42.86%), Postives = 77/126 (61.11%), Query Frame = 0


Query: 7   LAFSFLILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNV 66
           +A + L    AA  V+A    VG N+ WNP INYT+WA +  F++ D++ F Y++ QYNV
Sbjct: 8   VAGAVLAFLLAATNVTAKRWTVGDNKFWNPNINYTIWAQDKHFYLDDWLYFVYERNQYNV 67

Query: 67  FEVNQSGYGNCTTEGAFGNWS--SGKDFILLKEAKRYYFICGN-GQCYNGMKVTILVHPL 126
            EVN++ Y +C       NWS  +G+D + L   + YY I GN G CY GMK+ +LV   
Sbjct: 68  IEVNETNYISCNPNNPIANWSRGAGRDLVHLNVTRHYYLISGNGGGCYGGMKLAVLVEKP 127

Query: 127 PPPPSS 130
           PPPP++
Sbjct: 128 PPPPAA 133

[Close]

BLAST of MS016224.1 vs. TAIR 10
Match: AT4G12880.1 (early nodulin-like protein 19 )

HSP 1 Score: 104.8 bits (260), Expect = 7.1e-23
Identity = 50/121 (41.32%), Postives = 76/121 (62.81%), Query Frame = 0


Query: 13  ILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFEVNQS 72
           ++AA    V+A  ++VG  + WNP INYTLWA    F+VGD++ F + + Q+N+ EVN++
Sbjct: 16  LVAAPIPEVTAKKYLVGDKKFWNPNINYTLWAQGKHFYVGDWLYFVFYRDQHNILEVNKA 75

Query: 73  GYGNCTTEGAFGNWS--SGKDFILLKEAKRYYFICGNGQCYNGMKVTILVH-PLPPPPSS 131
            Y  C +     N++  +G+D + L E +RYY + G G C  GMK+ +LV  P PPPP +
Sbjct: 76  DYEKCISNRPIRNYTRGAGRDIVPLYETRRYYLLDGRGGCVQGMKLDVLVETPPPPPPFT 135

[Close]

BLAST of MS016224.1 vs. TAIR 10
Match: AT2G27035.1 (early nodulin-like protein 20 )

HSP 1 Score: 92.0 bits (227), Expect = 4.8e-19
Identity = 46/132 (34.85%), Postives = 69/132 (52.27%), Query Frame = 0


Query: 12  LILAAAAAVVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFEVNQ 71
           +IL     V S+   + G    WN  +N++ WAN+  F+ GD++ F + + ++N+ +VN+
Sbjct: 15  MILIIVVEVESSLHRVGGGRYTWNSDVNFSDWANHQRFYSGDWLYFGFNRTRHNILQVNK 74

Query: 72  SGYGNCT-TEGAFGNWSSGKDFILLKEAKRYYFICGNGQCYNGMKVTILVHPLPPPPSSP 131
           S Y  C   +  F     G+D   L E K YYFICG G C  GMK+ I V P PPP +  
Sbjct: 75  SSYEQCVDNDYIFNITRGGRDVFQLLEPKPYYFICGRGYCLKGMKLAITVLPQPPPSAPT 134

Query: 132 LAAKHSSPSSAP 143
                ++P   P
Sbjct: 135 NFTSTTTPLIPP 146

[Close]

BLAST of MS016224.1 vs. TAIR 10
Match: AT3G17675.1 (Cupredoxin superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.5e-17
Identity = 40/100 (40.00%), Postives = 57/100 (57.00%), Query Frame = 0


Query: 24  TDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKIQYNVFEVNQSGYGNCTTEGAF 83
           T+HIVG + GW    NYT W     F VGD + F Y+  Q+NV +VN + Y +C  +   
Sbjct: 7   TEHIVGDSNGWELFTNYTNWTQGREFHVGDVLVFNYKSDQHNVMQVNSTAYTDCGLDNYT 66

Query: 84  GNWSSGKDFILLKEAKRYYFICG-NGQCYNGMKVTILVHP 123
             ++ G D I+L E  + +FICG +  C NG K++I V P
Sbjct: 67  TLFTKGNDSIILSEVGKLWFICGVDDHCVNGQKLSINVAP 106

The following BLAST results are available for this feature:

BLAST of MS016224.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153463.1	2.8e-93	100.00	lamin-like protein [Momordica charantia]	[more]
XP_023526524.1	4.1e-73	83.33	lamin-like protein [Cucurbita pepo subsp. pepo]	[more]
KAG7018121.1	2.7e-72	82.74	Lamin-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038903873.1	4.6e-72	80.95	lamin-like protein [Benincasa hispida]	[more]
XP_022934432.1	1.0e-71	82.14	lamin-like protein [Cucurbita moschata]	[more]

BLAST of MS016224.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39131	3.7e-24	34.81	Lamin-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g15350 PE=2 SV=1	[more]
G7L0H3	9.1e-15	32.92	Blue copper protein 1a OS=Medicago truncatula OX=3880 GN=MTR_7g086140 PE=2 SV=1	[more]
A0A072U307	9.1e-15	32.30	Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1	[more]
Q41001	2.2e-13	36.69	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
A0A0M4FTF3	2.5e-12	38.46	Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1	[more]

BLAST of MS016224.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHI7	1.3e-93	100.00	lamin-like protein OS=Momordica charantia OX=3673 GN=LOC111020966 PE=4 SV=1	[more]
A0A6J1F2R0	4.9e-72	82.14	lamin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441613 PE=4 SV=1	[more]
A0A6J1J7T1	8.4e-72	84.47	lamin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482049 PE=4 SV=1	[more]
A0A1S3CN34	1.5e-68	77.98	lamin-like protein OS=Cucumis melo OX=3656 GN=LOC103502815 PE=4 SV=1	[more]
A0A0A0LCI9	3.4e-65	75.60	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G710810...	[more]

BLAST of MS016224.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15350.1	2.6e-25	34.81	early nodulin-like protein 17	[more]
AT3G01070.1	1.7e-24	42.86	early nodulin-like protein 16	[more]
AT4G12880.1	7.1e-23	41.32	early nodulin-like protein 19	[more]
AT2G27035.1	4.8e-19	34.85	early nodulin-like protein 20	[more]
AT3G17675.1	4.5e-17	40.00	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 34..114 e-value: 1.6E-18 score: 66.5
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 24..122 score: 33.209221
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 22..123 e-value: 2.3E-28 score: 100.5
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 23..121
None	No IPR available	PANTHER	PTHR33021:SF13	OS09G0557900 PROTEIN	coord: 10..146
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 10..146

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS016224	MS016224	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS016224.1-cds	MS016224.1-cds-scaffold9_2:1189155..1189495	CDS
MS016224.1-cds	MS016224.1-cds-scaffold9_2:1189634..1189799	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS016224.1	MS016224.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0009055	electron transfer activity