MS015688.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS015688.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin-protein ligase RING1-like
Location	scaffold983: 274617 .. 275294 (-)
Sequence length	678
RNA-Seq Expression	MS015688.1
Synteny	MS015688.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGGATACTCTCTACGACTACCATGTCTGGCAACCTCACGAAGACGAAGAACACGATAATGTTACCATTTCCAATTCTCACTTGCTTCCTTGCGACTGTTTCCTTCTCCATCTTCACTTTCGCCATGTTCTTCACTCAAATTCCCCTCAAGAATCATCGCACACACTCCTAAATTCGTCTCTATCCTCCTTCTTGATTCCTTGCGACGCCTTCTTTGCCCGCGCCTCCACTATTCTGATCGCCGTCTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCGGCGATTCCTGACGTCTTGTCGGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATGCTTCCGCTGGCGGTGTCCGTTTTGGTCGTCTCGCCGCACGATGAACGCGAGGAAATTGGGCGGGTTTCGCGGGAATCGGCTCAGGCGTTTAAGACGTGTCCGGCGAGTGAGTCGTCGATCGAAGCCTTGAAGACGGTGAAGATTGATGCCGGCCGGGAGTTGTTGATCGGAGAATGCAGGATATGCTTGGATGAACTGATGAGCGGAATGGAAGTTACGCGATTGCCGTGCTCTCATGTTTACCACAGAGACTGTATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGC

mRNA sequence

ATGGCCATGGATACTCTCTACGACTACCATGTCTGGCAACCTCACGAAGACGAAGAACACGATAATGTTACCATTTCCAATTCTCACTTGCTTCCTTGCGACTGTTTCCTTCTCCATCTTCACTTTCGCCATGTTCTTCACTCAAATTCCCCTCAAGAATCATCGCACACACTCCTAAATTCGTCTCTATCCTCCTTCTTGATTCCTTGCGACGCCTTCTTTGCCCGCGCCTCCACTATTCTGATCGCCGTCTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCGGCGATTCCTGACGTCTTGTCGGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATGCTTCCGCTGGCGGTGTCCGTTTTGGTCGTCTCGCCGCACGATGAACGCGAGGAAATTGGGCGGGTTTCGCGGGAATCGGCTCAGGCGTTTAAGACGTGTCCGGCGAGTGAGTCGTCGATCGAAGCCTTGAAGACGGTGAAGATTGATGCCGGCCGGGAGTTGTTGATCGGAGAATGCAGGATATGCTTGGATGAACTGATGAGCGGAATGGAAGTTACGCGATTGCCGTGCTCTCATGTTTACCACAGAGACTGTATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGC

Coding sequence (CDS)

ATGGCCATGGATACTCTCTACGACTACCATGTCTGGCAACCTCACGAAGACGAAGAACACGATAATGTTACCATTTCCAATTCTCACTTGCTTCCTTGCGACTGTTTCCTTCTCCATCTTCACTTTCGCCATGTTCTTCACTCAAATTCCCCTCAAGAATCATCGCACACACTCCTAAATTCGTCTCTATCCTCCTTCTTGATTCCTTGCGACGCCTTCTTTGCCCGCGCCTCCACTATTCTGATCGCCGTCTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCGGCGATTCCTGACGTCTTGTCGGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATGCTTCCGCTGGCGGTGTCCGTTTTGGTCGTCTCGCCGCACGATGAACGCGAGGAAATTGGGCGGGTTTCGCGGGAATCGGCTCAGGCGTTTAAGACGTGTCCGGCGAGTGAGTCGTCGATCGAAGCCTTGAAGACGGTGAAGATTGATGCCGGCCGGGAGTTGTTGATCGGAGAATGCAGGATATGCTTGGATGAACTGATGAGCGGAATGGAAGTTACGCGATTGCCGTGCTCTCATGTTTACCACAGAGACTGTATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGC

Protein sequence

MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLNSSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRMLPLAVSVLVVSPHDEREEIGRVSRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECRICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS

Homology

[Close]

BLAST of MS015688.1 vs. NCBI nr
Match: XP_022141616.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia] >XP_022141617.1 E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 458.8 bits (1179), Expect = 2.8e-125
Identity = 224/226 (99.12%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN
Sbjct: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM
Sbjct: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECR 180
           LPLAVSVLVVSPHDEREEIGR+SRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECR
Sbjct: 121 LPLAVSVLVVSPHDEREEIGRISRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECR 180

Query: 181 ICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYA+PLS
Sbjct: 181 ICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYALPLS 226

[Close]

BLAST of MS015688.1 vs. NCBI nr
Match: XP_022990569.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima] >XP_022990570.1 probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima])

HSP 1 Score: 380.9 bits (977), Expect = 7.4e-102
Identity = 191/227 (84.14%), Postives = 204/227 (89.87%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDY +WQP E E+  NVTISNSHLLPCDCFL HLHFRHVL SN PQE    LLN
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQR-LLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFA   +ILIAVFS TGVAQDFLDAA+PDVLSVAADIACNPLNAGRR+
Sbjct: 61  SSLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRV 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPLAVSVL VSP++EREEIGRV RES+ QAFKT PASES+IE LKTV+ID G ELLIGEC
Sbjct: 121 LPLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDELM+GMEVTRLPC+H+YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. NCBI nr
Match: KAG6602099.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032803.1 E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380.9 bits (977), Expect = 7.4e-102
Identity = 191/227 (84.14%), Postives = 204/227 (89.87%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDY +WQP E E+  NVTISNSHLLPCDCFL HLHFRHVL SN+PQE    LLN
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNNPQELQR-LLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFA   +ILIAVFS TGVAQDFLDAA+PDVLSVAADIACNPLNAGRR+
Sbjct: 61  SSLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRV 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPLAVSVL VSP++EREEIGRV RES+ QAFKT PASES+IE LKTV+ID G ELLIGEC
Sbjct: 121 LPLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDELM+GMEVTRLPC+H YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. NCBI nr
Match: XP_023554199.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.2 bits (975), Expect = 1.3e-101
Identity = 191/227 (84.14%), Postives = 203/227 (89.43%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDY +WQP E E+  NVTISNSHLLPCDCFL HLHFRHVL SN PQE    LLN
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDINVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQR-LLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFA   +ILIAVFS TGVAQDFLDAA+PDVLSVAADIACNPLNAGRR+
Sbjct: 61  SSLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRV 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPLAVSVL VSP++EREEIGRV RES+ QAFKT PASES+IE LKTV+ID G ELLIGEC
Sbjct: 121 LPLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDELM+GMEVTRLPC+H YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. NCBI nr
Match: XP_022963379.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata] >XP_022963389.1 probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata])

HSP 1 Score: 378.3 bits (970), Expect = 4.8e-101
Identity = 190/227 (83.70%), Postives = 202/227 (88.99%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDY +WQP E E+  NVTISNSHLLPCDCFL HLHFRHVL SN PQE    LLN
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQR-LLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFA   +ILIAVFS TGVAQDFLDAA+PDVLSVAADIACNPLNAGRR+
Sbjct: 61  SSLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRV 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPLAVSVL VSP++EREEIGRV RES+ Q FKT PASES+IE LKTV+ID G ELLIGEC
Sbjct: 121 LPLAVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDELM+GMEVTRLPC+H YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.1e-15
Identity = 34/89 (38.20%), Postives = 60/89 (67.42%), Query Frame = 0


Query: 139 IGRVSRESAQAFKTCPASESSIEALKTVKIDAGRELL---IGECRICLDELMSGMEVTRL 198
           I +++      + T PAS+S+I+AL TVK+   +++L   + +C +C+DE   G +V ++
Sbjct: 175 IQQLAENDPNRYGTPPASKSAIDALPTVKVT--KDMLKSEMNQCAVCMDEFEDGSDVKQM 234

Query: 199 PCSHVYHRDCIVKWLETSHLCPLCRYAMP 225
           PC HV+H+DC++ WLE  + CP+CR+ +P
Sbjct: 235 PCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of MS015688.1 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.1e-14
Identity = 45/136 (33.09%), Postives = 73/136 (53.68%), Query Frame = 0


Query: 94  FLDAAIPDVLSVAADIAC----NPLNAGRRMLPLAVSVLVVSPHDEREEIGRVSRESAQA 153
           F+   + D+ S  A I      NP + G R LP  +    + P  E + I +++      
Sbjct: 136 FIQNHLNDLRSSGAQIEFVIQNNPSDQGFR-LPANIGDYFIGPGLE-QLIQQLAENDPNR 195

Query: 154 FKTCPASESSIEALKTVKI-DAGRELLIGECRICLDELMSGMEVTRLPCSHVYHRDCIVK 213
           + T PAS+S+IEAL  V I  +       +C +C+D+   G E  ++PC H+YH+DC++ 
Sbjct: 196 YGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLP 255

Query: 214 WLETSHLCPLCRYAMP 225
           WLE  + CP+CR+ +P
Sbjct: 256 WLELHNSCPVCRHELP 269

[Close]

BLAST of MS015688.1 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.3e-12
Identity = 29/70 (41.43%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 154 PASESSIEALKTVKIDAGRELLIGECRICLDELMSGMEVTRLPCSHVYHRDCIVKWLETS 213
           PA++S+IEAL+T ++ +    ++  C +C D ++ G    +LPC H YH DCIV WL T 
Sbjct: 232 PAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLGTR 291

Query: 214 HLCPLCRYAM 224
           + CP+CR+ +
Sbjct: 292 NSCPVCRFQL 301

[Close]

BLAST of MS015688.1 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.3e-12
Identity = 38/113 (33.63%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 116 AGRRMLPLAVSVLVVSPHDER--EEIGRV--SRESAQAFKTCPASESSIEALKTVKIDAG 175
           +G R LP +VS +++    ER  E++ ++  S          PAS+S+IE+L  V+I   
Sbjct: 148 SGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDC 207

Query: 176 RELLIGECRICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMP 225
                  C +C +   +G+E   +PC H++H DCIV WL   + CP+CR+ +P
Sbjct: 208 HTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of MS015688.1 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.6e-12
Identity = 34/73 (46.58%), Postives = 46/73 (63.01%), Query Frame = 0


Query: 154 PASESSIEALKTVKIDAGRELLIGE--CRICLDELMSGMEVTRLPCSHVYHRDCIVKWLE 213
           PA  S+I+AL T+KI A R L   +  C +C DE   G E  ++PC+H+YH DCIV WL 
Sbjct: 164 PAPRSAIDALPTIKI-AQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLV 223

Query: 214 TSHLCPLCRYAMP 225
             + CP+CR  +P
Sbjct: 224 QHNSCPVCRQELP 235

[Close]

BLAST of MS015688.1 vs. ExPASy TrEMBL
Match: A0A6J1CJQ8 (E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC111011930 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.4e-125
Identity = 224/226 (99.12%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN
Sbjct: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM
Sbjct: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECR 180
           LPLAVSVLVVSPHDEREEIGR+SRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECR
Sbjct: 121 LPLAVSVLVVSPHDEREEIGRISRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECR 180

Query: 181 ICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYA+PLS
Sbjct: 181 ICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYALPLS 226

[Close]

BLAST of MS015688.1 vs. ExPASy TrEMBL
Match: A0A6J1JSE1 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111487409 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.6e-102
Identity = 191/227 (84.14%), Postives = 204/227 (89.87%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDY +WQP E E+  NVTISNSHLLPCDCFL HLHFRHVL SN PQE    LLN
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQR-LLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFA   +ILIAVFS TGVAQDFLDAA+PDVLSVAADIACNPLNAGRR+
Sbjct: 61  SSLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRV 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPLAVSVL VSP++EREEIGRV RES+ QAFKT PASES+IE LKTV+ID G ELLIGEC
Sbjct: 121 LPLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDELM+GMEVTRLPC+H+YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. ExPASy TrEMBL
Match: A0A6J1HJY0 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC111463602 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.3e-101
Identity = 190/227 (83.70%), Postives = 202/227 (88.99%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDY +WQP E E+  NVTISNSHLLPCDCFL HLHFRHVL SN PQE    LLN
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQR-LLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCDAFFA   +ILIAVFS TGVAQDFLDAA+PDVLSVAADIACNPLNAGRR+
Sbjct: 61  SSLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRV 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPLAVSVL VSP++EREEIGRV RES+ Q FKT PASES+IE LKTV+ID G ELLIGEC
Sbjct: 121 LPLAVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDELM+GMEVTRLPC+H YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. ExPASy TrEMBL
Match: A0A0A0KDU3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.8e-101
Identity = 187/227 (82.38%), Postives = 204/227 (89.87%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDYH+WQP E E+  NVTISNSHLLPC+CFL HLHFRH+L SN  Q+  H LLN
Sbjct: 1   MAMDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQ-FHYLLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSSFLIPCD FFA AS+ILIAVFSGTGVAQDFLD A+PDVLSVAADIACNPLNAGR +
Sbjct: 61  SSLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTI 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPLAVSVLVVSP++ER+EIGRV RES+ QAFKT PASE +IE LK V+ID G ELLIGEC
Sbjct: 121 LPLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKKVEIDVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDELM+GMEVTRLPC+H+YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. ExPASy TrEMBL
Match: A0A5A7V2F0 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G001370 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 8.3e-99
Identity = 182/227 (80.18%), Postives = 203/227 (89.43%), Query Frame = 0


Query: 1   MAMDTLYDYHVWQPHEDEEHDNVTISNSHLLPCDCFLLHLHFRHVLHSNSPQESSHTLLN 60
           MAMDTLYDYH+WQP E E+  NVTISNSHLLPC+CFL HLHFRH+L SN  Q+ SH LLN
Sbjct: 1   MAMDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQ-SHYLLN 60

Query: 61  SSLSSFLIPCDAFFARASTILIAVFSGTGVAQDFLDAAIPDVLSVAADIACNPLNAGRRM 120
           SSLSS+LIPCD FFA AS+ILIAVFSGTGVAQDFLD A+PDVLSVAADIACNPLNAGR +
Sbjct: 61  SSLSSYLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTI 120

Query: 121 LPLAVSVLVVSPHDEREEIGRVSRESA-QAFKTCPASESSIEALKTVKIDAGRELLIGEC 180
           LPL VSVLVVSP++ER+EIG+V RES+ QAFKT PASE +IE LK V+I  G ELLIGEC
Sbjct: 121 LPLTVSVLVVSPYNERQEIGQVLRESSPQAFKTLPASEMAIEDLKNVEIGVGGELLIGEC 180

Query: 181 RICLDELMSGMEVTRLPCSHVYHRDCIVKWLETSHLCPLCRYAMPLS 227
           RICLDEL++GME+TRLPC+H+YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 RICLDELVNGMEITRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of MS015688.1 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 5.1e-16
Identity = 34/89 (38.20%), Postives = 60/89 (67.42%), Query Frame = 0


Query: 139 IGRVSRESAQAFKTCPASESSIEALKTVKIDAGRELL---IGECRICLDELMSGMEVTRL 198
           I +++      + T PAS+S+I+AL TVK+   +++L   + +C +C+DE   G +V ++
Sbjct: 175 IQQLAENDPNRYGTPPASKSAIDALPTVKVT--KDMLKSEMNQCAVCMDEFEDGSDVKQM 234

Query: 199 PCSHVYHRDCIVKWLETSHLCPLCRYAMP 225
           PC HV+H+DC++ WLE  + CP+CR+ +P
Sbjct: 235 PCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of MS015688.1 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.8e-14
Identity = 34/84 (40.48%), Postives = 51/84 (60.71%), Query Frame = 0


Query: 141 RVSRESAQAFKTCPASESSIEALKTVKIDAGRELLIGECRICLDELMSGMEVTRLPCSHV 200
           R++      + T PA + ++EAL TVKI+   E L  +C +CLD+   G E   +PC+H 
Sbjct: 190 RLAENDPNRYGTPPAKKEAVEALATVKIE---ETL--QCSVCLDDFEIGTEAKLMPCTHK 249

Query: 201 YHRDCIVKWLETSHLCPLCRYAMP 225
           +H DC++ WLE    CP+CRY +P
Sbjct: 250 FHSDCLLPWLELHSSCPVCRYQLP 268

[Close]

BLAST of MS015688.1 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 4.7e-14
Identity = 32/73 (43.84%), Postives = 48/73 (65.75%), Query Frame = 0


Query: 154 PASESSIEALKTVKIDAGRELL-IGECRICLDELMSGMEVTRLPCSHVYHRDCIVKWLET 213
           PASE +I +L +VKI        + +C +C++E + G + T LPC H+YH+DCIV WL  
Sbjct: 198 PASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRL 257

Query: 214 SHLCPLCRYAMPL 226
           ++ CP+CR  +PL
Sbjct: 258 NNSCPICRRDLPL 270

[Close]

BLAST of MS015688.1 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 4.7e-14
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 150 FKTCPASESSIEALKTVKIDAGRELLIGECRICLDELMSGMEVTRLPCSHVYHRDCIVKW 209
           + T PA++ ++EAL  VKI+      + +C +CLD+   GME   +PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIEDS----LLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 210 LETSHLCPLCRYAMP 225
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

[Close]

BLAST of MS015688.1 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 4.7e-14
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 150 FKTCPASESSIEALKTVKIDAGRELLIGECRICLDELMSGMEVTRLPCSHVYHRDCIVKW 209
           + T PA++ ++EAL  VKI+      + +C +CLD+   GME   +PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIEDS----LLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 210 LETSHLCPLCRYAMP 225
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

The following BLAST results are available for this feature:

BLAST of MS015688.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141616.1	2.8e-125	99.12	E3 ubiquitin-protein ligase RING1-like [Momordica charantia] >XP_022141617.1 E3 ...	[more]
XP_022990569.1	7.4e-102	84.14	probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima] >XP_022990570.1 pr...	[more]
KAG6602099.1	7.4e-102	84.14	E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023554199.1	1.3e-101	84.14	probable E3 ubiquitin-protein ligase ATL45 [Cucurbita pepo subsp. pepo]	[more]
XP_022963379.1	4.8e-101	83.70	probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata] >XP_022963389.1 ...	[more]

BLAST of MS015688.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	7.1e-15	38.20	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	2.1e-14	33.09	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9SPL2	4.3e-12	41.43	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV...	[more]
Q9SNB6	4.3e-12	33.63	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
O22197	5.6e-12	46.58	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of MS015688.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJQ8	1.4e-125	99.12	E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1JSE1	3.6e-102	84.14	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1HJY0	2.3e-101	83.70	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0KDU3	6.8e-101	82.38	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 P...	[more]
A0A5A7V2F0	8.3e-99	80.18	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of MS015688.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	5.1e-16	38.20	RING/U-box superfamily protein	[more]
AT1G55530.1	2.8e-14	40.48	RING/U-box superfamily protein	[more]
AT1G60360.1	4.7e-14	43.84	RING/U-box superfamily protein	[more]
AT3G13430.1	4.7e-14	41.33	RING/U-box superfamily protein	[more]
AT3G13430.2	4.7e-14	41.33	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 178..220 e-value: 0.002 score: 13.2
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 179..219 e-value: 3.1E-7 score: 40.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 178..220 e-value: 3.8E-14 score: 52.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 179..220 score: 13.020386
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 140..225 e-value: 3.9E-21 score: 76.5
None	No IPR available	PANTHER	PTHR15710:SF120	F6A14.12 PROTEIN-RELATED	coord: 79..224
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 79..224
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 165..224

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS015688	MS015688	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS015688.1-cds	MS015688.1-cds-scaffold983:274617..275294	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS015688.1	MS015688.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding