MS015328.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS015328.1 vs. NCBI nr
Match: XP_022150550.1 (uncharacterized protein LOC111018665 [Momordica charantia])

HSP 1 Score: 771.5 bits (1991), Expect = 3.7e-219
Identity = 431/436 (98.85%), Postives = 432/436 (99.08%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL
Sbjct: 57  MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 116

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF
Sbjct: 117 FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 176

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN
Sbjct: 177 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 236

Query: 181 GITLKNLEDYGVPTE--KKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLKNLEDYGVPTE  KKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK
Sbjct: 237 GITLKNLEDYGVPTEVQKKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 296

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAA AARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE
Sbjct: 297 KYKDVADAAQAAFESAAYAAVAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 356

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
           I+LESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL
Sbjct: 357 IELESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 416

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS
Sbjct: 417 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 476

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAVLRTIEVRGH
Sbjct: 477 IGGNEAVLRTIEVRGH 492

BLAST of MS015328.1 vs. NCBI nr
Match: XP_038893459.1 (uncharacterized protein LOC120082250 isoform X2 [Benincasa hispida])

HSP 1 Score: 629.4 bits (1622), Expect = 2.3e-176
Identity = 358/434 (82.49%), Postives = 389/434 (89.63%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVR+S A SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRASHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVV ERIKLLE ERECPEE+KE ISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKLLEHERECPEEIKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVITSRFGKEFTARAIELRNNCAV PMIVQKLS RP SLEIKLKLLKQIASE+
Sbjct: 121 PELIEIRSVITSRFGKEFTARAIELRNNCAVGPMIVQKLSARPSSLEIKLKLLKQIASEH 180

Query: 181 GITLKNLEDYGVPTEKKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRKKY 240
           GITLK+LE Y V TE++V  EEK+EQPQQETK E V Q LPEEIEKDDRYADSMRGRKKY
Sbjct: 181 GITLKDLEAYEVSTEERVVAEEKKEQPQQETKVESVFQILPEEIEKDDRYADSMRGRKKY 240

Query: 241 KDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTEIK 300
           KDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD DD SS S +PR+S+ET ESKN +++
Sbjct: 241 KDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSSPSSKPRKSTETLESKNADME 300

Query: 301 LESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELLEK 360
           L+SENKET GGSTSIE EESE+ IE KQP+SSSSSE+GDAS KENEIPIEAIN+I+LLEK
Sbjct: 301 LKSENKETHGGSTSIEAEESESGIESKQPISSSSSEAGDASLKENEIPIEAINIIDLLEK 360

Query: 361 DLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLSIE 420
           DL FDESDVD GNGES S T EE NS  +C  K+ESESAEIT + +ESDNKNT QNLSI 
Sbjct: 361 DLAFDESDVDAGNGESSSSTLEERNSCFEC-HKIESESAEITANNDESDNKNTAQNLSI- 420

Query: 421 GNEAVLRTIEVRGH 435
           GNEAV+ TI+VRGH
Sbjct: 421 GNEAVITTIDVRGH 432

BLAST of MS015328.1 vs. NCBI nr
Match: XP_038893458.1 (uncharacterized protein LOC120082250 isoform X1 [Benincasa hispida])

HSP 1 Score: 624.4 bits (1609), Expect = 7.4e-175
Identity = 358/436 (82.11%), Postives = 389/436 (89.22%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVR+S A SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRASHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVV ERIKLLE ERECPEE+KE ISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKLLEHERECPEEIKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVITSRFGKEFTARAIELRNNCAV PMIVQKLS RP SLEIKLKLLKQIASE+
Sbjct: 121 PELIEIRSVITSRFGKEFTARAIELRNNCAVGPMIVQKLSARPSSLEIKLKLLKQIASEH 180

Query: 181 GITLKNLEDYGVPTE--KKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLK+LE Y V TE  ++V  EEK+EQPQQETK E V Q LPEEIEKDDRYADSMRGRK
Sbjct: 181 GITLKDLEAYEVSTEMQERVVAEEKKEQPQQETKVESVFQILPEEIEKDDRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD DD SS S +PR+S+ET ESKN +
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSSPSSKPRKSTETLESKNAD 300

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
           ++L+SENKET GGSTSIE EESE+ IE KQP+SSSSSE+GDAS KENEIPIEAIN+I+LL
Sbjct: 301 MELKSENKETHGGSTSIEAEESESGIESKQPISSSSSEAGDASLKENEIPIEAINIIDLL 360

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDL FDESDVD GNGES S T EE NS  +C  K+ESESAEIT + +ESDNKNT QNLS
Sbjct: 361 EKDLAFDESDVDAGNGESSSSTLEERNSCFEC-HKIESESAEITANNDESDNKNTAQNLS 420

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAV+ TI+VRGH
Sbjct: 421 I-GNEAVITTIDVRGH 434

BLAST of MS015328.1 vs. NCBI nr
Match: XP_004135100.1 (uncharacterized protein LOC101220964 [Cucumis sativus] >KGN52067.1 hypothetical protein Csa_008786 [Cucumis sativus])

HSP 1 Score: 618.6 bits (1594), Expect = 4.0e-173
Identity = 359/436 (82.34%), Postives = 386/436 (88.53%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVRSSQA SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVV ERIK LE ERECPEELKE ISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVIT+RFGKEFTARAIELRNNC+V PMIVQKLS R  SLEIKLKLLKQIASE+
Sbjct: 121 PELIEIRSVITTRFGKEFTARAIELRNNCSVGPMIVQKLSARSASLEIKLKLLKQIASEH 180

Query: 181 GITLKNLEDYGVPTEKKVEV--EEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLK+LE Y   TE+KV V  EE +EQPQQETKEEGV Q LPEEIEKD+RYADSMRGRK
Sbjct: 181 GITLKDLEAYEASTEEKVMVMAEETKEQPQQETKEEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD DD S+LSP+PR+S+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
            ++   NKET GGS+SIEVEESEN IELKQPVSSSSSE+GDAS KENEIPIEAIN+IELL
Sbjct: 301 KEM---NKETHGGSSSIEVEESENEIELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDL FDESDVD  NGES +   EE N S    EKMESESAEIT + EESDN +T QN S
Sbjct: 361 EKDLTFDESDVDAANGESSNSALEEKNLSFVSCEKMESESAEITANNEESDNNDTAQNTS 420

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAV+RTI+VRGH
Sbjct: 421 I-GNEAVIRTIDVRGH 432

BLAST of MS015328.1 vs. NCBI nr
Match: XP_008446583.1 (PREDICTED: midasin [Cucumis melo] >KAA0034542.1 midasin [Cucumis melo var. makuwa] >TYK09096.1 midasin [Cucumis melo var. makuwa])

HSP 1 Score: 610.5 bits (1573), Expect = 1.1e-170
Identity = 355/436 (81.42%), Postives = 384/436 (88.07%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVRSSQA SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVV ERIK LE +RECPEELKE ISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVIT+RFGKEFTARAIELRNNCAV PMIVQKLS RP SLEIKLKLLKQIASE+
Sbjct: 121 PELIEIRSVITNRFGKEFTARAIELRNNCAVGPMIVQKLSARPASLEIKLKLLKQIASEH 180

Query: 181 GITLKNLEDYGVPTEKKVEV--EEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLK+LEDY V +E+KV V  EEK+E+PQQETK EGV Q LPEEIEKD+RYADSMRGRK
Sbjct: 181 GITLKDLEDYEVSSEEKVVVMAEEKKEEPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD DD S+LSP+PR+S+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
            ++  E      GSTSIE EESEN  ELKQPVSSSSSE+GDAS KENEIPIEAIN+I LL
Sbjct: 301 KEMNKEE----SGSTSIE-EESENGTELKQPVSSSSSEAGDASLKENEIPIEAINIIGLL 360

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDL FDESDVD   GES     EE N S + REKMESESAEITT+ EESDN +T QN+S
Sbjct: 361 EKDLAFDESDVDAAKGESSGSAIEERNLSFESREKMESESAEITTNNEESDNDDTAQNIS 420

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAV+RTI+VRGH
Sbjct: 421 I-GNEAVIRTIDVRGH 430

BLAST of MS015328.1 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.4e-13
Identity = 55/189 (29.10%), Postives = 95/189 (50.26%), Query Frame = 0

Query: 8   LLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVEQVI 67
           +LG  FK  R R  + L I+RL +L  ++   + +A  ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRC-GDFPELLEI 127
           +E  L++A  I+E YC+++  R  L++  +E    L E +S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASENGITLKN 187
              + +++ KE+  +         V+  ++ KLSV  P   +  + L +IA         
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIA--------- 178

Query: 188 LEDYGVPTE 196
            ++Y VP E
Sbjct: 181 -KNYNVPYE 178

BLAST of MS015328.1 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 5.8e-13
Identity = 67/234 (28.63%), Postives = 122/234 (52.14%), Query Frame = 0

Query: 10  GRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVEQVIKE 69
           G S+ + + +  + LA+SR+ +L N++         +V +LL+  ++E A  RVE +I++
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  QKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDFPELLEIRSV 129
           + L++ + IIE  CE++  RI L+    E P E+KE I  L+++S R    PEL +I++ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 ITSRFGKEFTARAIELRNNC----AVSPMIVQKLSVRPPSLEIKLKLLKQIASENGITLK 189
           + +++GK      +E   NC     V+P IV KLS   P   I  + L +IA +  +   
Sbjct: 125 LKAKYGK-----GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWC 184

Query: 190 NLEDYGVPTE----KKVEVEEK----QEQPQ--QETKEEGVLQSLPEEIEKDDR 230
              DY  P +    + + V+++    Q  PQ     ++  +LQ  P+ I++  +
Sbjct: 185 G-SDYPPPPQLIMPQPIIVQQQPQILQPPPQIIHHQQQPQILQPPPQIIQQQQQ 231

BLAST of MS015328.1 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.5e-13
Identity = 57/195 (29.23%), Postives = 97/195 (49.74%), Query Frame = 0

Query: 8   LLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVEQVI 67
           +LG  FK  R R  + L I+RL +L  ++   + +A  ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRC-GDFPELLEI 127
           +E  L++A  I+E YC+++  R  L++  +E    L E +S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASENGITLKN 187
              + +++ KE+  +         V+  ++ KLSV  P   +  + L +IA    +  + 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE- 180

Query: 188 LEDYGVPTEKKVEVE 202
             D  V  E  V VE
Sbjct: 181 -PDSVVMAEAPVGVE 192

BLAST of MS015328.1 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.5e-13
Identity = 57/195 (29.23%), Postives = 97/195 (49.74%), Query Frame = 0

Query: 8   LLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVEQVI 67
           +LG  FK  R R  + L I+RL +L  ++   + +A  ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRC-GDFPELLEI 127
           +E  L++A  I+E YC+++  R  L++  +E    L E +S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASENGITLKN 187
              + +++ KE+  +         V+  ++ KLSV  P   +  + L +IA    +  + 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE- 180

Query: 188 LEDYGVPTEKKVEVE 202
             D  V  E  V VE
Sbjct: 181 -PDSVVMAEAPVGVE 192

BLAST of MS015328.1 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-12
Identity = 54/189 (28.57%), Postives = 94/189 (49.74%), Query Frame = 0

Query: 8   LLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVEQVI 67
           +LG   K  R R  + L I+RL +L  ++   + +A  ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRC-GDFPELLEI 127
           +E  L++A  I+E YC+++  R  L++  +E    L E +S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASENGITLKN 187
              + +++ KE+  +         V+  ++ KLSV  P   +  + L +IA         
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIA--------- 178

Query: 188 LEDYGVPTE 196
            ++Y VP E
Sbjct: 181 -KNYNVPYE 178

BLAST of MS015328.1 vs. ExPASy TrEMBL
Match: A0A6J1D8S6 (uncharacterized protein LOC111018665 OS=Momordica charantia OX=3673 GN=LOC111018665 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.8e-219
Identity = 431/436 (98.85%), Postives = 432/436 (99.08%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL
Sbjct: 57  MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 116

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF
Sbjct: 117 FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 176

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN
Sbjct: 177 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 236

Query: 181 GITLKNLEDYGVPTE--KKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLKNLEDYGVPTE  KKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK
Sbjct: 237 GITLKNLEDYGVPTEVQKKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 296

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAA AARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE
Sbjct: 297 KYKDVADAAQAAFESAAYAAVAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 356

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
           I+LESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL
Sbjct: 357 IELESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 416

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS
Sbjct: 417 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 476

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAVLRTIEVRGH
Sbjct: 477 IGGNEAVLRTIEVRGH 492

BLAST of MS015328.1 vs. ExPASy TrEMBL
Match: A0A0A0KUG8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608550 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 2.0e-173
Identity = 359/436 (82.34%), Postives = 386/436 (88.53%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVRSSQA SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVV ERIK LE ERECPEELKE ISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVIT+RFGKEFTARAIELRNNC+V PMIVQKLS R  SLEIKLKLLKQIASE+
Sbjct: 121 PELIEIRSVITTRFGKEFTARAIELRNNCSVGPMIVQKLSARSASLEIKLKLLKQIASEH 180

Query: 181 GITLKNLEDYGVPTEKKVEV--EEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLK+LE Y   TE+KV V  EE +EQPQQETKEEGV Q LPEEIEKD+RYADSMRGRK
Sbjct: 181 GITLKDLEAYEASTEEKVMVMAEETKEQPQQETKEEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD DD S+LSP+PR+S+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
            ++   NKET GGS+SIEVEESEN IELKQPVSSSSSE+GDAS KENEIPIEAIN+IELL
Sbjct: 301 KEM---NKETHGGSSSIEVEESENEIELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDL FDESDVD  NGES +   EE N S    EKMESESAEIT + EESDN +T QN S
Sbjct: 361 EKDLTFDESDVDAANGESSNSALEEKNLSFVSCEKMESESAEITANNEESDNNDTAQNTS 420

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAV+RTI+VRGH
Sbjct: 421 I-GNEAVIRTIDVRGH 432

BLAST of MS015328.1 vs. ExPASy TrEMBL
Match: A0A5A7STU8 (Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00860 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 5.3e-171
Identity = 355/436 (81.42%), Postives = 384/436 (88.07%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVRSSQA SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVV ERIK LE +RECPEELKE ISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVIT+RFGKEFTARAIELRNNCAV PMIVQKLS RP SLEIKLKLLKQIASE+
Sbjct: 121 PELIEIRSVITNRFGKEFTARAIELRNNCAVGPMIVQKLSARPASLEIKLKLLKQIASEH 180

Query: 181 GITLKNLEDYGVPTEKKVEV--EEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLK+LEDY V +E+KV V  EEK+E+PQQETK EGV Q LPEEIEKD+RYADSMRGRK
Sbjct: 181 GITLKDLEDYEVSSEEKVVVMAEEKKEEPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD DD S+LSP+PR+S+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
            ++  E      GSTSIE EESEN  ELKQPVSSSSSE+GDAS KENEIPIEAIN+I LL
Sbjct: 301 KEMNKEE----SGSTSIE-EESENGTELKQPVSSSSSEAGDASLKENEIPIEAINIIGLL 360

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDL FDESDVD   GES     EE N S + REKMESESAEITT+ EESDN +T QN+S
Sbjct: 361 EKDLAFDESDVDAAKGESSGSAIEERNLSFESREKMESESAEITTNNEESDNDDTAQNIS 420

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAV+RTI+VRGH
Sbjct: 421 I-GNEAVIRTIDVRGH 430

BLAST of MS015328.1 vs. ExPASy TrEMBL
Match: A0A1S3BEW7 (midasin OS=Cucumis melo OX=3656 GN=LOC103489273 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 5.3e-171
Identity = 355/436 (81.42%), Postives = 384/436 (88.07%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVRSSQA SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVV ERIK LE +RECPEELKE ISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVIT+RFGKEFTARAIELRNNCAV PMIVQKLS RP SLEIKLKLLKQIASE+
Sbjct: 121 PELIEIRSVITNRFGKEFTARAIELRNNCAVGPMIVQKLSARPASLEIKLKLLKQIASEH 180

Query: 181 GITLKNLEDYGVPTEKKVEV--EEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           GITLK+LEDY V +E+KV V  EEK+E+PQQETK EGV Q LPEEIEKD+RYADSMRGRK
Sbjct: 181 GITLKDLEDYEVSSEEKVVVMAEEKKEEPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD DD S+LSP+PR+S+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
            ++  E      GSTSIE EESEN  ELKQPVSSSSSE+GDAS KENEIPIEAIN+I LL
Sbjct: 301 KEMNKEE----SGSTSIE-EESENGTELKQPVSSSSSEAGDASLKENEIPIEAINIIGLL 360

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDL FDESDVD   GES     EE N S + REKMESESAEITT+ EESDN +T QN+S
Sbjct: 361 EKDLAFDESDVDAAKGESSGSAIEERNLSFESREKMESESAEITTNNEESDNDDTAQNIS 420

Query: 421 IEGNEAVLRTIEVRGH 435
           I GNEAV+RTI+VRGH
Sbjct: 421 I-GNEAVIRTIDVRGH 430

BLAST of MS015328.1 vs. ExPASy TrEMBL
Match: A0A6J1HWT1 (uncharacterized protein LOC111467561 OS=Cucurbita maxima OX=3661 GN=LOC111467561 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.3e-169
Identity = 351/436 (80.50%), Postives = 384/436 (88.07%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKTTR +ALVNLAISRLAVLANQRQVR + A SDV+QLL+LGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRHTHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
            RVEQVIKEQKLLDAYAIIESYCEVV ERIK LE ERECPEELKE ISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL+EIRSVI SRFGK+FTA+AIELRNNCAVSPMIVQKLS RPPSLEIK+KLLKQIASEN
Sbjct: 121 PELVEIRSVIASRFGKDFTAKAIELRNNCAVSPMIVQKLSARPPSLEIKMKLLKQIASEN 180

Query: 181 GITLKNLEDYGVPTE--KKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRK 240
           G+TLK+LE Y   TE  +KV  EE ++QP QETK EGV Q LPEEIEKD RYADSMRGR+
Sbjct: 181 GVTLKDLEAYEDSTEMQEKVVAEENKDQPAQETKVEGVFQILPEEIEKDSRYADSMRGRR 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVEL+QSKSLD+DD ++LS +PR+SSET ESKN E
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDQDDLNTLSTKPRKSSETDESKNIE 300

Query: 301 IKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAINVIELL 360
           I+LESENK    GSTSIEVEESE+  EL QPVSSSSSE+GD S K+NEIPIEAIN+IELL
Sbjct: 301 IELESENKVIHMGSTSIEVEESESPAELMQPVSSSSSEAGDVSLKDNEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESISPTFEEINSSIQCREKMESESAEITTHIEESDNKNTTQNLS 420
           EKDL FDESDVD GNGES S T EE NSS Q  +KMESESAEI THIEESDNK ++++LS
Sbjct: 361 EKDLAFDESDVDAGNGESSSSTLEEKNSSFQSCKKMESESAEIITHIEESDNK-SSEHLS 420

Query: 421 IEGNEAVLRTIEVRGH 435
           I G+EAV+RT +VRGH
Sbjct: 421 I-GDEAVVRTTDVRGH 434

BLAST of MS015328.1 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 245.4 bits (625), Expect = 8.7e-65
Identity = 186/445 (41.80%), Postives = 264/445 (59.33%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERAL 60
           MGKKLDALLGRSFKT + ++L+ LA++RL++L NQRQ R SQA SDV +LLKLG  E A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDF 120
            RV+QV+K+Q  LD    I  Y  +  +RI L E  R+CPEEL E +SGL+FA+SR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELLEIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASEN 180
           PEL EIR+V+ SRFGK+  AR+IELR+NC V P I+QKLS RPP  E+++K LK+IA+EN
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

Query: 181 GITLKNLEDYGVPTEKKVEVEEKQEQPQQE-TKEEGVLQSLPEEIEKDDRY--ADSM-RG 240
            I LK L+     TE    ++   +  + + T ++G          + + Y  +DS+ RG
Sbjct: 181 NIVLK-LDQASTSTEGTTNMQGTSDVSKTKLTSKDG----------RGEGYGLSDSVKRG 240

Query: 241 RKKYKDVADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQESKN 300
           +KKYKDVADAAQAAFESAA+AA AA+AAVEL+Q      D P ++               
Sbjct: 241 KKKYKDVADAAQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGENSFHG------- 300

Query: 301 TEIKLESENKETLGGSTSIEVEESENAIELKQPVSSSSSESGDASSKENEIPIEAI--NV 360
                 SENKE     +  E E ++++ E +  V S S  S   S    ++P+ +   + 
Sbjct: 301 ------SENKE-----SEQEQEGNDDSSEGEGDVMSESKRSMSDSEDIIDVPVMSFREDP 360

Query: 361 IELLEKDLVFDESDVDTGNGESISPTFE--EINSSIQCREKMESESAEITTHIE---ESD 420
           ++LLEKD +  +S+ +T       P+ E   I+ S   + +M   +   T H++    S 
Sbjct: 361 VKLLEKDTIIYDSEEET------QPSVETITISKSKDEQNRMVGSNRADTRHVDHMVRSI 409

Query: 421 NKNTTQNLSIEGNEAVLRTIEVRGH 435
                +   ++G  +V RT +VRG+
Sbjct: 421 EDPIMRKAGLKGPVSV-RTRQVRGY 409

BLAST of MS015328.1 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 140.6 bits (353), Expect = 3.0e-33
Identity = 84/242 (34.71%), Postives = 143/242 (59.09%), Query Frame = 0

Query: 5   LDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVE 64
           LD+   + FK  +C+ L+ L I R+ ++ N+R+ +  Q   ++ +LL+ G +  A  RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  QVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDFPELL 124
            +I+E+K++ A  I+E +CE++A R+ ++E +RECP +LKE IS + FA+ RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASENGITL 184
           +++ +  S++GKEF A A EL+ +  V+  +V+ LSVR PS E KLKLLK+IA E+ +  
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHEL-- 183

Query: 185 KNLEDYGVPTEKKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRKKYKDVA 244
               D+  P   + ++ +  E      K+ G    LP   E++++   +     K K  +
Sbjct: 184 ----DWD-PASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDS 238

Query: 245 DA 247
           D+
Sbjct: 244 DS 238

BLAST of MS015328.1 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 140.6 bits (353), Expect = 3.0e-33
Identity = 94/289 (32.53%), Postives = 154/289 (53.29%), Query Frame = 0

Query: 7   ALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVEQV 66
           +L  R F +++C+    +A++R+ ++ N+R V   Q   D+  LL+ G    A  RVE V
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 67  IKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDFPELLEI 126
           I+EQ +  A  IIE +CE++  R+ ++ K+++CP +LKE I+ LIFA+ RC + PEL ++
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 127 RSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASENGI---T 186
           R +   ++GK+F + A +LR +C V+ M++ KLSVR P  E KLK++K+IA E  +   T
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDT 199

Query: 187 LKNLEDYGVPTEKKVEVEEKQEQPQQETKEEGVLQSLPEEIEKDDRYADSMRGRKKYKDV 246
            +  ++   P E+ ++   K             +    +  +   R   SM     Y D 
Sbjct: 200 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDT 259

Query: 247 ADAAQAAFESAAYAAAAARAAVELAQSKSLDRDDPSSLSPRPRRSSETQ 293
             AA+AA E A  A AAA+ A  LA  +     + S  S       ++Q
Sbjct: 260 ESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQ 308

BLAST of MS015328.1 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.4e-30
Identity = 96/298 (32.21%), Postives = 156/298 (52.35%), Query Frame = 0

Query: 5   LDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVE 64
           L+ L  R     +C+  +NLAI+R+ +L N+R ++      ++   L+ G +  A  RVE
Sbjct: 4   LNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVE 63

Query: 65  QVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDFPELL 124
            VI+E  L  AY I+E +CE +  R+ +LE E+ECP EL+E I+ +IFA+ RC + P+LL
Sbjct: 64  HVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLL 123

Query: 125 EIRSVITSRFGKEFTARAIELRNNCAVSPMIVQKLSVRPPSLEIKLKLLKQIASENGIT- 184
           +I+++  +++GKEF   A ELR +  V+  I++KLS   PS   +LK+LK+IA E  +  
Sbjct: 124 QIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNW 183

Query: 185 ---------LKNLEDY-----GVPTEKKVEVEEKQEQPQQETKEEGVLQSLPEE------ 244
                    +K+ ED       +  +  +      +Q   ++     ++SLP E      
Sbjct: 184 DSSATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 243

Query: 245 -IEKDDRYADSMRG-------------RKKYKDVADAAQAAFESAAYAAAAARAAVEL 268
            ++  +  + SM               R+   DV + A+AA  SA  A AAARAA +L
Sbjct: 244 KLQAQNPVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQL 301

BLAST of MS015328.1 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 84/272 (30.88%), Postives = 143/272 (52.57%), Query Frame = 0

Query: 5   LDALLGRSFKTTRCRALVNLAISRLAVLANQRQVRSSQAHSDVIQLLKLGHQERALFRVE 64
           LD+   + FK  +C+ L+ L I R+ ++ N+R+ +  Q   ++ +LL+ G +  A  RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  QVIKEQKLLDAYAIIESYCEVVAERIKLLEKERECPEELKEPISGLIFASSRCGDFPELL 124
            +I+E+K++ A  I+E +CE++A R+ ++E +RECP +LKE IS + FA+ RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIRSVITSRFGKEFTARAIELRNNCAVSP------------------------------M 184
           +++ +  S++GKEF A A EL+ +  V+                                
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQ 183

Query: 185 IVQKLSVRPPSLEIKLKLLKQIASENGITLKNLEDYGVPTEKKVEVEEKQEQPQQETKEE 244
           +V+ LSVR PS E KLKLLK+IA E+ +      D+  P   + ++ +  E      K+ 
Sbjct: 184 LVELLSVRAPSPETKLKLLKEIAEEHEL------DWD-PASTETDLFKSHEDLLDGPKQF 243

Query: 245 GVLQSLPEEIEKDDRYADSMRGRKKYKDVADA 247
           G    LP   E++++   +     K K  +D+
Sbjct: 244 GGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDS 268