MS014642.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS014642.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ethylene-responsive transcription factor RAP2-3-like
Location	scaffold1096: 271872 .. 272818 (+)
Sequence length	621
RNA-Seq Expression	MS014642.1
Synteny	MS014642.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCGGCGGAGCCATCATCTCCGATTTCGTTGATACGACCTGCCATCAGAGCCCAGTCGTGGACGATAACCTCTGGCCTGAGTTCGACACCTTCTCCGAGCTCCTCGGTCTCGACGCCTCGACTGGAAACTACCTTTTCAACAACTCTCCGACCAACAACAACCTCCAACAACATTCTCACGGTACACTATCATTCCCTCCTTAATTCCTCTTCATTTAAATTTTCTTTTTAATTAATTTTATCTCCCTTGATATCACGTCAAACTTTCATTAACAATAAACTTAAAACCAACAAGTTATAAATATATATCTAATTTTCTAAAACCTTATATTCTCTTTTAACTCTCGCTGTCAACACAAAACTCAACAAGAACTAATTGGGCGAAAAATGAACAATATTTCTTACTACCCATCACTAAAATCTCTTTTAAGCACGAATTTTCTAGTATCCAGTTAGTGCGTGTTCGATAGCGATGGTAAAAATAGTAATTTTGAAATAACATTCCAGTGAAAAGTGAGAAGAAAGAAGAATGTGAGGAAGCAGGAGGGGAAGTTGCAGAGGCAGTGGCAGAGGAGATGAAGAAACGACCGCAGAAACAGAGAGTTCGGAAGAGCAAATACAGAGGAATCCGGCAGCGGCCATGGGGGAAATGGGCGGCGGAGATTCGAGACCCACACAAAGGACTCCGCGTCTGGCTCGGAACCTTCAACACCGCCGAAGAAGCAGCCGCAGCTTACGACCAAGCCGCCATACGAATCAGAGGAAAAAAGGCCAAACTCAACTTCACCCACCCACCGCCGTCTCCTCCGCCGCCGCCCGCCGTCCAACTCTCAAGCCTGGAATCCTTTCTGGGCCTGGACCCCTCGCCGCCGCCGGTGGAGCCCATGGCCGACCTCTGGTGGGCCGATGACCTTCTCACATATCACCACCAAAATCTCCCCCTC

mRNA sequence

ATGTGCGGCGGAGCCATCATCTCCGATTTCGTTGATACGACCTGCCATCAGAGCCCAGTCGTGGACGATAACCTCTGGCCTGAGTTCGACACCTTCTCCGAGCTCCTCGGTCTCGACGCCTCGACTGGAAACTACCTTTTCAACAACTCTCCGACCAACAACAACCTCCAACAACATTCTCACGTGAAAAGTGAGAAGAAAGAAGAATGTGAGGAAGCAGGAGGGGAAGTTGCAGAGGCAGTGGCAGAGGAGATGAAGAAACGACCGCAGAAACAGAGAGTTCGGAAGAGCAAATACAGAGGAATCCGGCAGCGGCCATGGGGGAAATGGGCGGCGGAGATTCGAGACCCACACAAAGGACTCCGCGTCTGGCTCGGAACCTTCAACACCGCCGAAGAAGCAGCCGCAGCTTACGACCAAGCCGCCATACGAATCAGAGGAAAAAAGGCCAAACTCAACTTCACCCACCCACCGCCGTCTCCTCCGCCGCCGCCCGCCGTCCAACTCTCAAGCCTGGAATCCTTTCTGGGCCTGGACCCCTCGCCGCCGCCGGTGGAGCCCATGGCCGACCTCTGGTGGGCCGATGACCTTCTCACATATCACCACCAAAATCTCCCCCTC

Coding sequence (CDS)

ATGTGCGGCGGAGCCATCATCTCCGATTTCGTTGATACGACCTGCCATCAGAGCCCAGTCGTGGACGATAACCTCTGGCCTGAGTTCGACACCTTCTCCGAGCTCCTCGGTCTCGACGCCTCGACTGGAAACTACCTTTTCAACAACTCTCCGACCAACAACAACCTCCAACAACATTCTCACGTGAAAAGTGAGAAGAAAGAAGAATGTGAGGAAGCAGGAGGGGAAGTTGCAGAGGCAGTGGCAGAGGAGATGAAGAAACGACCGCAGAAACAGAGAGTTCGGAAGAGCAAATACAGAGGAATCCGGCAGCGGCCATGGGGGAAATGGGCGGCGGAGATTCGAGACCCACACAAAGGACTCCGCGTCTGGCTCGGAACCTTCAACACCGCCGAAGAAGCAGCCGCAGCTTACGACCAAGCCGCCATACGAATCAGAGGAAAAAAGGCCAAACTCAACTTCACCCACCCACCGCCGTCTCCTCCGCCGCCGCCCGCCGTCCAACTCTCAAGCCTGGAATCCTTTCTGGGCCTGGACCCCTCGCCGCCGCCGGTGGAGCCCATGGCCGACCTCTGGTGGGCCGATGACCTTCTCACATATCACCACCAAAATCTCCCCCTC

Protein sequence

MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQNLPL

Homology

[Close]

BLAST of MS014642.1 vs. NCBI nr
Match: XP_022137925.1 (ethylene-responsive transcription factor RAP2-3-like [Momordica charantia])

HSP 1 Score: 424.1 bits (1089), Expect = 7.0e-115
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHS 60
           MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHS
Sbjct: 1   MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHS 60

Query: 61  HVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDPHKG 120
           HVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDP KG
Sbjct: 61  HVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDPQKG 120

Query: 121 LRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLESFLGLDP 180
           LRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLESFLGLDP
Sbjct: 121 LRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLESFLGLDP 180

Query: 181 SPPPVEPMADLWWADDLLTYHHQNLPL 208
           SPPPVEPMADLWWADDLLTYHHQNLPL
Sbjct: 181 SPPPVEPMADLWWADDLLTYHHQNLPL 207

[Close]

BLAST of MS014642.1 vs. NCBI nr
Match: XP_038906638.1 (ethylene-responsive transcription factor RAP2-3 [Benincasa hispida])

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-59
Identity = 146/233 (62.66%), Postives = 160/233 (68.67%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFD-TFSELLGLDASTGNYLFNNSPTN---NN 60
           MCGGAIISD+V+     +PVV DN LWP  D TFS+LLGLD S  NY FNN+P+N     
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPNLDTTFSDLLGLDLSGQNYPFNNTPSNVVAET 60

Query: 61  LQQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIR 120
            +Q S VKSEK E  E  GG          KK+PQK  +RKSKYRGIRQRPWGKWAAEIR
Sbjct: 61  PKQLSQVKSEKVEGNEAVGG----------KKKPQK--IRKSKYRGIRQRPWGKWAAEIR 120

Query: 121 DPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPP-PSPPP--PPAV----- 180
           DP KGLRVWLGTFNTAEEAA AYDQAAIRIRGKKAK NFTHPP P+  P  PP++     
Sbjct: 121 DPQKGLRVWLGTFNTAEEAARAYDQAAIRIRGKKAKTNFTHPPSPTSLPLLPPSLPQQQL 180

Query: 181 --------------QLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQNLP 207
                         Q+S+LESFLGLDPS P VEPMADLWW DDL TY  QN P
Sbjct: 181 PTTSSPSFDIELKDQISTLESFLGLDPS-PLVEPMADLWWVDDLFTYQQQNFP 216

[Close]

BLAST of MS014642.1 vs. NCBI nr
Match: KAA0049520.1 (ethylene-responsive transcription factor RAP2-3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 240.0 bits (611), Expect = 1.9e-59
Identity = 148/244 (60.66%), Postives = 164/244 (67.21%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFDT--FSELLGLDAS-TGNYLFNNSPTNNNL 60
           MCGGAIISD+V+     +PVV DN LWP+ DT  FS+LLGLD S T NYLFNN+  + N+
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPDLDTTLFSDLLGLDLSGTQNYLFNNTTPSANV 60

Query: 61  -----QQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWA 120
                +Q S VK EK E  E A  E+         K+PQK  VRKSKYRGIRQRPWGKWA
Sbjct: 61  VHNIPKQLSQVKKEKVEGNEGATSEM---------KKPQK--VRKSKYRGIRQRPWGKWA 120

Query: 121 AEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSP------PPPP 180
           AEIRDP KGLRVWLGT+NTAEEAA AYDQAAIRIRGKKAKLNF HPPP P      P PP
Sbjct: 121 AEIRDPQKGLRVWLGTYNTAEEAARAYDQAAIRIRGKKAKLNFPHPPPPPSLPLPLPLPP 180

Query: 181 AV----------------------QLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQ 208
           ++                      Q+S+LESFLGLDPS PPVEP ADLWW DDLLTY  Q
Sbjct: 181 SLPQQQQQQLPTTSATPFDVELKHQISTLESFLGLDPS-PPVEPTADLWWVDDLLTYQQQ 228

[Close]

BLAST of MS014642.1 vs. NCBI nr
Match: XP_008438910.1 (PREDICTED: ethylene-responsive transcription factor RAP2-3 isoform X1 [Cucumis melo])

HSP 1 Score: 238.8 bits (608), Expect = 4.2e-59
Identity = 147/244 (60.25%), Postives = 164/244 (67.21%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFDT--FSELLGLDAS-TGNYLFNNSPTNNNL 60
           MCGGAIISD+V+     +PVV DN LWP+ DT  FS+LLGLD S T NYLFNN+  + N+
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPDLDTTLFSDLLGLDLSGTQNYLFNNTTPSANV 60

Query: 61  -----QQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWA 120
                +Q S VK E+ E  E A  E+         K+PQK  VRKSKYRGIRQRPWGKWA
Sbjct: 61  VHNIPKQLSQVKKERVEGNEGATSEM---------KKPQK--VRKSKYRGIRQRPWGKWA 120

Query: 121 AEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSP------PPPP 180
           AEIRDP KGLRVWLGT+NTAEEAA AYDQAAIRIRGKKAKLNF HPPP P      P PP
Sbjct: 121 AEIRDPQKGLRVWLGTYNTAEEAARAYDQAAIRIRGKKAKLNFPHPPPPPSLPLPLPLPP 180

Query: 181 AV----------------------QLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQ 208
           ++                      Q+S+LESFLGLDPS PPVEP ADLWW DDLLTY  Q
Sbjct: 181 SLPQQQQQQLPTTSATPFDVELKHQISTLESFLGLDPS-PPVEPTADLWWVDDLLTYQQQ 228

[Close]

BLAST of MS014642.1 vs. NCBI nr
Match: XP_008438911.1 (PREDICTED: ethylene-responsive transcription factor RAP2-3 isoform X2 [Cucumis melo])

HSP 1 Score: 235.0 bits (598), Expect = 6.0e-58
Identity = 144/239 (60.25%), Postives = 161/239 (67.36%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFDT--FSELLGLDAS-TGNYLFNNSPTNNNL 60
           MCGGAIISD+V+     +PVV DN LWP+ DT  FS+LLGLD S T NYLFNN+  + N+
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPDLDTTLFSDLLGLDLSGTQNYLFNNTTPSANV 60

Query: 61  QQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRD 120
             H+  K   +E  E   G  +E       K+PQK  VRKSKYRGIRQRPWGKWAAEIRD
Sbjct: 61  -VHNIPKQLSQERVEGNEGATSE------MKKPQK--VRKSKYRGIRQRPWGKWAAEIRD 120

Query: 121 PHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSP------PPPPAV--- 180
           P KGLRVWLGT+NTAEEAA AYDQAAIRIRGKKAKLNF HPPP P      P PP++   
Sbjct: 121 PQKGLRVWLGTYNTAEEAARAYDQAAIRIRGKKAKLNFPHPPPPPSLPLPLPLPPSLPQQ 180

Query: 181 -------------------QLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQNLPL 208
                              Q+S+LESFLGLDPS PPVEP ADLWW DDLLTY  QNL L
Sbjct: 181 QQQQLPTTSATPFDVELKHQISTLESFLGLDPS-PPVEPTADLWWVDDLLTYQQQNLQL 225

[Close]

BLAST of MS014642.1 vs. ExPASy Swiss-Prot
Match: P42736 (Ethylene-responsive transcription factor RAP2-3 OS=Arabidopsis thaliana OX=3702 GN=RAP2-3 PE=1 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 2.3e-28
Identity = 84/172 (48.84%), Postives = 100/172 (58.14%), Query Frame = 0


Query: 1   MCGGAIISDFVD--TTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQ 60
           MCGGAIISD+    T      +  + LW E         LDAS  +  +    T+   + 
Sbjct: 1   MCGGAIISDYAPLVTKAKGRKLTAEELWSE---------LDASAADDFWGFYSTS---KL 60

Query: 61  HSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDPH 120
           H   +   KE          EAV +E    P K+R RK+ YRGIR+RPWGKWAAEIRDP 
Sbjct: 61  HPTNQVNVKE----------EAVKKEQATEPGKRRKRKNVYRGIRKRPWGKWAAEIRDPR 120

Query: 121 KGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTH----PPPSPPPPPA 167
           KG+RVWLGTFNTAEEAA AYD AA +IRG KAKLNF      PPP+  PPP+
Sbjct: 121 KGVRVWLGTFNTAEEAAMAYDVAAKQIRGDKAKLNFPDLHHPPPPNYTPPPS 150

[Close]

BLAST of MS014642.1 vs. ExPASy Swiss-Prot
Match: Q70II3 (Ethylene-responsive transcription factor ERF110 OS=Arabidopsis thaliana OX=3702 GN=ERF110 PE=2 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 4.5e-24
Identity = 56/95 (58.95%), Postives = 69/95 (72.63%), Query Frame = 0


Query: 81  VAEEMKKR-PQKQRVRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYD 140
           + E M K  P++ R +K +YRG+RQRPWGKWAAEIRDPH+  RVWLGTF+TAE AA AYD
Sbjct: 58  IEESMSKAFPEEAREKKRRYRGVRQRPWGKWAAEIRDPHRAARVWLGTFDTAEAAARAYD 117

Query: 141 QAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLES 175
           +AA+R RG KAKLNF       PPPP +  S  ++
Sbjct: 118 EAALRFRGNKAKLNFPEDVRILPPPPPLLRSPADT 152

[Close]

BLAST of MS014642.1 vs. ExPASy Swiss-Prot
Match: Q6K7E6 (Ethylene-responsive transcription factor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=EREBP1 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 51/67 (76.12%), Postives = 58/67 (86.57%), Query Frame = 0


Query: 92  QRVRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAK 151
           +R RK+++RGIRQRPWGKWAAEIRDP KG+RVWLGTFN+AEEAA AYD  A RIRGKKAK
Sbjct: 113 KRKRKNQFRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNSAEEAARAYDAEARRIRGKKAK 172

Query: 152 LNFTHPP 159
           +NF   P
Sbjct: 173 VNFPEAP 179

[Close]

BLAST of MS014642.1 vs. ExPASy Swiss-Prot
Match: O22259 (Ethylene-responsive transcription factor ERF071 OS=Arabidopsis thaliana OX=3702 GN=ERF071 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-22
Identity = 54/82 (65.85%), Postives = 64/82 (78.05%), Query Frame = 0


Query: 80  AVAEEMKKRP-------QKQRVRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAE 139
           +V+   K++P         +R RK+ YRGIRQRPWGKWAAEIRDP KG+RVWLGTF TA+
Sbjct: 24  SVSSRKKRKPVSVSEERDGKRERKNLYRGIRQRPWGKWAAEIRDPSKGVRVWLGTFKTAD 83

Query: 140 EAAAAYDQAAIRIRGKKAKLNF 155
           EAA AYD AAI+IRG+KAKLNF
Sbjct: 84  EAARAYDVAAIKIRGRKAKLNF 105

[Close]

BLAST of MS014642.1 vs. ExPASy Swiss-Prot
Match: Q7G1L2 (Ethylene-responsive transcription factor RAP2-6 OS=Arabidopsis thaliana OX=3702 GN=RAP2-6 PE=2 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 4.2e-22
Identity = 48/60 (80.00%), Postives = 50/60 (83.33%), Query Frame = 0


Query: 95  RKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNF 154
           R  KYRG+RQRPWGKWAAEIRDPHK  RVWLGTF TAE AA AYD AA+R RG KAKLNF
Sbjct: 57  RPKKYRGVRQRPWGKWAAEIRDPHKATRVWLGTFETAEAAARAYDAAALRFRGSKAKLNF 116

[Close]

BLAST of MS014642.1 vs. ExPASy TrEMBL
Match: A0A6J1C807 (ethylene-responsive transcription factor RAP2-3-like OS=Momordica charantia OX=3673 GN=LOC111009221 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 3.4e-115
Identity = 206/207 (99.52%), Postives = 206/207 (99.52%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHS 60
           MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHS
Sbjct: 1   MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHS 60

Query: 61  HVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDPHKG 120
           HVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDP KG
Sbjct: 61  HVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDPQKG 120

Query: 121 LRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLESFLGLDP 180
           LRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLESFLGLDP
Sbjct: 121 LRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLESFLGLDP 180

Query: 181 SPPPVEPMADLWWADDLLTYHHQNLPL 208
           SPPPVEPMADLWWADDLLTYHHQNLPL
Sbjct: 181 SPPPVEPMADLWWADDLLTYHHQNLPL 207

[Close]

BLAST of MS014642.1 vs. ExPASy TrEMBL
Match: A0A5A7U2G6 (Ethylene-responsive transcription factor RAP2-3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G007740 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 9.0e-60
Identity = 148/244 (60.66%), Postives = 164/244 (67.21%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFDT--FSELLGLDAS-TGNYLFNNSPTNNNL 60
           MCGGAIISD+V+     +PVV DN LWP+ DT  FS+LLGLD S T NYLFNN+  + N+
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPDLDTTLFSDLLGLDLSGTQNYLFNNTTPSANV 60

Query: 61  -----QQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWA 120
                +Q S VK EK E  E A  E+         K+PQK  VRKSKYRGIRQRPWGKWA
Sbjct: 61  VHNIPKQLSQVKKEKVEGNEGATSEM---------KKPQK--VRKSKYRGIRQRPWGKWA 120

Query: 121 AEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSP------PPPP 180
           AEIRDP KGLRVWLGT+NTAEEAA AYDQAAIRIRGKKAKLNF HPPP P      P PP
Sbjct: 121 AEIRDPQKGLRVWLGTYNTAEEAARAYDQAAIRIRGKKAKLNFPHPPPPPSLPLPLPLPP 180

Query: 181 AV----------------------QLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQ 208
           ++                      Q+S+LESFLGLDPS PPVEP ADLWW DDLLTY  Q
Sbjct: 181 SLPQQQQQQLPTTSATPFDVELKHQISTLESFLGLDPS-PPVEPTADLWWVDDLLTYQQQ 228

[Close]

BLAST of MS014642.1 vs. ExPASy TrEMBL
Match: A0A1S3AY54 (ethylene-responsive transcription factor RAP2-3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483869 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 2.0e-59
Identity = 147/244 (60.25%), Postives = 164/244 (67.21%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFDT--FSELLGLDAS-TGNYLFNNSPTNNNL 60
           MCGGAIISD+V+     +PVV DN LWP+ DT  FS+LLGLD S T NYLFNN+  + N+
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPDLDTTLFSDLLGLDLSGTQNYLFNNTTPSANV 60

Query: 61  -----QQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWA 120
                +Q S VK E+ E  E A  E+         K+PQK  VRKSKYRGIRQRPWGKWA
Sbjct: 61  VHNIPKQLSQVKKERVEGNEGATSEM---------KKPQK--VRKSKYRGIRQRPWGKWA 120

Query: 121 AEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSP------PPPP 180
           AEIRDP KGLRVWLGT+NTAEEAA AYDQAAIRIRGKKAKLNF HPPP P      P PP
Sbjct: 121 AEIRDPQKGLRVWLGTYNTAEEAARAYDQAAIRIRGKKAKLNFPHPPPPPSLPLPLPLPP 180

Query: 181 AV----------------------QLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQ 208
           ++                      Q+S+LESFLGLDPS PPVEP ADLWW DDLLTY  Q
Sbjct: 181 SLPQQQQQQLPTTSATPFDVELKHQISTLESFLGLDPS-PPVEPTADLWWVDDLLTYQQQ 228

[Close]

BLAST of MS014642.1 vs. ExPASy TrEMBL
Match: A0A1S3AY72 (ethylene-responsive transcription factor RAP2-3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483869 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.9e-58
Identity = 144/239 (60.25%), Postives = 161/239 (67.36%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFDT--FSELLGLDAS-TGNYLFNNSPTNNNL 60
           MCGGAIISD+V+     +PVV DN LWP+ DT  FS+LLGLD S T NYLFNN+  + N+
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPDLDTTLFSDLLGLDLSGTQNYLFNNTTPSANV 60

Query: 61  QQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRD 120
             H+  K   +E  E   G  +E       K+PQK  VRKSKYRGIRQRPWGKWAAEIRD
Sbjct: 61  -VHNIPKQLSQERVEGNEGATSE------MKKPQK--VRKSKYRGIRQRPWGKWAAEIRD 120

Query: 121 PHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPSP------PPPPAV--- 180
           P KGLRVWLGT+NTAEEAA AYDQAAIRIRGKKAKLNF HPPP P      P PP++   
Sbjct: 121 PQKGLRVWLGTYNTAEEAARAYDQAAIRIRGKKAKLNFPHPPPPPSLPLPLPLPPSLPQQ 180

Query: 181 -------------------QLSSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQNLPL 208
                              Q+S+LESFLGLDPS PPVEP ADLWW DDLLTY  QNL L
Sbjct: 181 QQQQLPTTSATPFDVELKHQISTLESFLGLDPS-PPVEPTADLWWVDDLLTYQQQNLQL 225

[Close]

BLAST of MS014642.1 vs. ExPASy TrEMBL
Match: A0A0A0L7Z6 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G164580 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.3e-55
Identity = 144/241 (59.75%), Postives = 156/241 (64.73%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDN-LWPEFD-TFSELLGLD--ASTGNYLFNNSPT---- 60
           MCGGAIISD+V+     +PVV DN LWP+ D TFS+LLGLD    T NY FNN+ +    
Sbjct: 1   MCGGAIISDYVE----PNPVVVDNLLWPDLDTTFSDLLGLDLITQTQNYPFNNTTSPNVV 60

Query: 61  -NNNLQQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWA 120
            NN  +Q S VK EK E  E   GE+         K+PQK  VRKSKYRGIRQRPWGKWA
Sbjct: 61  DNNIPKQLSQVKKEKVEGNEVGRGEM---------KKPQK--VRKSKYRGIRQRPWGKWA 120

Query: 121 AEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTHPPPS----------P 180
           AEIRDP KGLRVWLGT+NT EEAA AYDQAAIRIRGKKAKLNF  P PS          P
Sbjct: 121 AEIRDPRKGLRVWLGTYNTPEEAARAYDQAAIRIRGKKAKLNFPPPLPSPPLLSVPLSLP 180

Query: 181 PPPPAVQL---------------SSLESFLGLDPSPPPVEPMADLWWADDLLTYHHQNLP 208
           P PP  QL               S+LESFLGLDPS PPVEP ADLWW DDLL    QNL 
Sbjct: 181 PSPPQQQLPTTSTPIDVELKHQISTLESFLGLDPS-PPVEPTADLWWVDDLLACQQQNLQ 225

[Close]

BLAST of MS014642.1 vs. TAIR 10
Match: AT3G16770.1 (ethylene-responsive element binding protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.6e-29
Identity = 84/172 (48.84%), Postives = 100/172 (58.14%), Query Frame = 0


Query: 1   MCGGAIISDFVD--TTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQ 60
           MCGGAIISD+    T      +  + LW E         LDAS  +  +    T+   + 
Sbjct: 1   MCGGAIISDYAPLVTKAKGRKLTAEELWSE---------LDASAADDFWGFYSTS---KL 60

Query: 61  HSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQRVRKSKYRGIRQRPWGKWAAEIRDPH 120
           H   +   KE          EAV +E    P K+R RK+ YRGIR+RPWGKWAAEIRDP 
Sbjct: 61  HPTNQVNVKE----------EAVKKEQATEPGKRRKRKNVYRGIRKRPWGKWAAEIRDPR 120

Query: 121 KGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLNFTH----PPPSPPPPPA 167
           KG+RVWLGTFNTAEEAA AYD AA +IRG KAKLNF      PPP+  PPP+
Sbjct: 121 KGVRVWLGTFNTAEEAAMAYDVAAKQIRGDKAKLNFPDLHHPPPPNYTPPPS 150

[Close]

BLAST of MS014642.1 vs. TAIR 10
Match: AT5G50080.1 (ethylene response factor 110 )

HSP 1 Score: 112.8 bits (281), Expect = 3.2e-25
Identity = 56/95 (58.95%), Postives = 69/95 (72.63%), Query Frame = 0


Query: 81  VAEEMKKR-PQKQRVRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYD 140
           + E M K  P++ R +K +YRG+RQRPWGKWAAEIRDPH+  RVWLGTF+TAE AA AYD
Sbjct: 58  IEESMSKAFPEEAREKKRRYRGVRQRPWGKWAAEIRDPHRAARVWLGTFDTAEAAARAYD 117

Query: 141 QAAIRIRGKKAKLNFTHPPPSPPPPPAVQLSSLES 175
           +AA+R RG KAKLNF       PPPP +  S  ++
Sbjct: 118 EAALRFRGNKAKLNFPEDVRILPPPPPLLRSPADT 152

[Close]

BLAST of MS014642.1 vs. TAIR 10
Match: AT3G14230.2 (related to AP2 2 )

HSP 1 Score: 105.9 bits (263), Expect = 3.9e-23
Identity = 74/182 (40.66%), Postives = 90/182 (49.45%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDNLWP----------------------------EFDTF 60
           MCGGAIISDF+        V ++ +WP                            +F  F
Sbjct: 1   MCGGAIISDFIPPP-RSLRVTNEFIWPDLKNKVKASKKRSNKRSDFFDLDDDFEADFQGF 60

Query: 61  SELLGLDASTGNYLFNNSPTNNNLQQHSHVKSEKKEECEEAGGEVAEAVAEEMKKRPQKQ 120
            +    D    + +F N            V S        A  +     AE+ +K    +
Sbjct: 61  KDDSAFDCEDDDDVFVNVKPFVFTATTKPVASAFVSTVGSAYAKKTVESAEQAEK--SSK 120

Query: 121 RVRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKL 155
           R RK++YRGIRQRPWGKWAAEIRDP KG R WLGTF+TAEEAA AYD AA RIRG KAK+
Sbjct: 121 RKRKNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAKV 179

[Close]

BLAST of MS014642.1 vs. TAIR 10
Match: AT3G14230.3 (related to AP2 2 )

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 70/181 (38.67%), Postives = 93/181 (51.38%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDNLWPEFDTFSELLGLDASTGNYLFNNSPTNNNLQQHS 60
           MCGGAIISDF+        V ++ +WP+     +     ++  +  F+    ++      
Sbjct: 1   MCGGAIISDFIPPP-RSLRVTNEFIWPDLKNKVKASKKRSNKRSDFFDLD--DDFEADFQ 60

Query: 61  HVKSEKKEECEE--------------------------AGGEVAEAVAEEMKK-RPQKQR 120
             K +   +CE+                           G   A+   E  ++     +R
Sbjct: 61  GFKDDSAFDCEDDDDVFVNVKPFVFTATTKPVASAFVSTGSAYAKKTVESAEQAEKSSKR 120

Query: 121 VRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKAKLN 155
            RK++YRGIRQRPWGKWAAEIRDP KG R WLGTF+TAEEAA AYD AA RIRG KAK+N
Sbjct: 121 KRKNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAKVN 178

[Close]

BLAST of MS014642.1 vs. TAIR 10
Match: AT3G14230.1 (related to AP2 2 )

HSP 1 Score: 102.4 bits (254), Expect = 4.3e-22
Identity = 72/184 (39.13%), Postives = 89/184 (48.37%), Query Frame = 0


Query: 1   MCGGAIISDFVDTTCHQSPVVDDNLWP----------------------------EFDTF 60
           MCGGAIISDF+        V ++ +WP                            +F  F
Sbjct: 1   MCGGAIISDFIPPP-RSLRVTNEFIWPDLKNKVKASKKRSNKRSDFFDLDDDFEADFQGF 60

Query: 61  SELLGLDASTGNYLF-NNSPTNNNLQQHSHVKSEKKEECEEAGGEVAEAVAEEMKK-RPQ 120
            +    D    + +F N  P            +         G   A+   E  ++    
Sbjct: 61  KDDSAFDCEDDDDVFVNVKPFVFTATTKPVASAFVSTGIYLVGSAYAKKTVESAEQAEKS 120

Query: 121 KQRVRKSKYRGIRQRPWGKWAAEIRDPHKGLRVWLGTFNTAEEAAAAYDQAAIRIRGKKA 155
            +R RK++YRGIRQRPWGKWAAEIRDP KG R WLGTF+TAEEAA AYD AA RIRG KA
Sbjct: 121 SKRKRKNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKA 180

The following BLAST results are available for this feature:

BLAST of MS014642.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137925.1	7.0e-115	99.52	ethylene-responsive transcription factor RAP2-3-like [Momordica charantia]	[more]
XP_038906638.1	1.1e-59	62.66	ethylene-responsive transcription factor RAP2-3 [Benincasa hispida]	[more]
KAA0049520.1	1.9e-59	60.66	ethylene-responsive transcription factor RAP2-3 isoform X1 [Cucumis melo var. ma...	[more]
XP_008438910.1	4.2e-59	60.25	PREDICTED: ethylene-responsive transcription factor RAP2-3 isoform X1 [Cucumis m...	[more]
XP_008438911.1	6.0e-58	60.25	PREDICTED: ethylene-responsive transcription factor RAP2-3 isoform X2 [Cucumis m...	[more]

BLAST of MS014642.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42736	2.3e-28	48.84	Ethylene-responsive transcription factor RAP2-3 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q70II3	4.5e-24	58.95	Ethylene-responsive transcription factor ERF110 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6K7E6	2.9e-23	76.12	Ethylene-responsive transcription factor 1 OS=Oryza sativa subsp. japonica OX=39...	[more]
O22259	1.5e-22	65.85	Ethylene-responsive transcription factor ERF071 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q7G1L2	4.2e-22	80.00	Ethylene-responsive transcription factor RAP2-6 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of MS014642.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C807	3.4e-115	99.52	ethylene-responsive transcription factor RAP2-3-like OS=Momordica charantia OX=3...	[more]
A0A5A7U2G6	9.0e-60	60.66	Ethylene-responsive transcription factor RAP2-3 isoform X1 OS=Cucumis melo var. ...	[more]
A0A1S3AY54	2.0e-59	60.25	ethylene-responsive transcription factor RAP2-3 isoform X1 OS=Cucumis melo OX=36...	[more]
A0A1S3AY72	2.9e-58	60.25	ethylene-responsive transcription factor RAP2-3 isoform X2 OS=Cucumis melo OX=36...	[more]
A0A0A0L7Z6	2.3e-55	59.75	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G164580 PE=...	[more]

BLAST of MS014642.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16770.1	1.6e-29	48.84	ethylene-responsive element binding protein	[more]
AT5G50080.1	3.2e-25	58.95	ethylene response factor 110	[more]
AT3G14230.2	3.9e-23	40.66	related to AP2 2	[more]
AT3G14230.3	1.5e-22	38.67	related to AP2 2	[more]
AT3G14230.1	4.3e-22	39.13	related to AP2 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 63..83
None	No IPR available	PANTHER	PTHR31190:SF210	EREBP TRANSCRIPTION FACTOR	coord: 1..163
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 121..137 score: 69.95 coord: 99..110 score: 70.45
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 98..161 e-value: 1.9E-38 score: 143.7
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 97..147 e-value: 1.6E-14 score: 53.8
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 98..155 score: 23.656738
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 97..156 e-value: 4.60525E-22 score: 83.0899
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 97..156 e-value: 8.4E-27 score: 95.1
IPR044808	Ethylene-responsive transcription factor	PANTHER	PTHR31190	DNA-BINDING DOMAIN	coord: 1..163
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 97..155

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS014642	MS014642	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS014642.1-cds	MS014642.1-cds-scaffold1096:271872..272055	CDS
MS014642.1-cds	MS014642.1-cds-scaffold1096:272382..272818	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS014642.1	MS014642.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009873	ethylene-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity