MS014605.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS014605.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	60S ribosome subunit biogenesis protein NIP7 homolog
Location	scaffold1096: 31128 .. 31688 (+)
Sequence length	561
RNA-Seq Expression	MS014605.1
Synteny	MS014605.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACCTTTAGACGAGAAGGAGACCACCGCGGTATTCGAAAAGCTCCACAAATTCACCGGAAACAACCTCAGGAACATCGTCGAGAATCCCTCTCACGAAGGCCCCGAACCAAATCCTGGCCGCTACTGCTTCCGCCTCCACAAGAACAAGGTCTATTACGTCAGCGATTCCCTCGTCAAGCGCGCCACCAACGTAGCCCGCCGCAACCTCGTCGCTCTCGGCACCTGCATCGGCAAGATCACTCATGGCGGCAGTTTCCATCTCACTGTTCAGTCGCTAGGTCTCTTGGCCCCCAATGCGAAGCACAAGGTTTGGCTTAAACCTACCTCTGAGATGTCTTTTTTGTACGGGAACCACGTTTTGAAAGGCGGTCTAGGGCGGATTACTGAAAATATTGCTCCTGGTGATGGAGTCGTCGTGTATTCGATGTCGGACGTGCCTTTGGGGTTTGGAATTGCGGCGAAGTCCACGCAGGACTGTAGGAAATTGGATCCCAATGGGATTGTGGTTCTCCATCAGTCGGATATTGGCGAGTATTTGAGGATGGAAGATGAGCTT

mRNA sequence

ATGAGACCTTTAGACGAGAAGGAGACCACCGCGGTATTCGAAAAGCTCCACAAATTCACCGGAAACAACCTCAGGAACATCGTCGAGAATCCCTCTCACGAAGGCCCCGAACCAAATCCTGGCCGCTACTGCTTCCGCCTCCACAAGAACAAGGTCTATTACGTCAGCGATTCCCTCGTCAAGCGCGCCACCAACGTAGCCCGCCGCAACCTCGTCGCTCTCGGCACCTGCATCGGCAAGATCACTCATGGCGGCAGTTTCCATCTCACTGTTCAGTCGCTAGGTCTCTTGGCCCCCAATGCGAAGCACAAGGTTTGGCTTAAACCTACCTCTGAGATGTCTTTTTTGTACGGGAACCACGTTTTGAAAGGCGGTCTAGGGCGGATTACTGAAAATATTGCTCCTGGTGATGGAGTCGTCGTGTATTCGATGTCGGACGTGCCTTTGGGGTTTGGAATTGCGGCGAAGTCCACGCAGGACTGTAGGAAATTGGATCCCAATGGGATTGTGGTTCTCCATCAGTCGGATATTGGCGAGTATTTGAGGATGGAAGATGAGCTT

Coding sequence (CDS)

ATGAGACCTTTAGACGAGAAGGAGACCACCGCGGTATTCGAAAAGCTCCACAAATTCACCGGAAACAACCTCAGGAACATCGTCGAGAATCCCTCTCACGAAGGCCCCGAACCAAATCCTGGCCGCTACTGCTTCCGCCTCCACAAGAACAAGGTCTATTACGTCAGCGATTCCCTCGTCAAGCGCGCCACCAACGTAGCCCGCCGCAACCTCGTCGCTCTCGGCACCTGCATCGGCAAGATCACTCATGGCGGCAGTTTCCATCTCACTGTTCAGTCGCTAGGTCTCTTGGCCCCCAATGCGAAGCACAAGGTTTGGCTTAAACCTACCTCTGAGATGTCTTTTTTGTACGGGAACCACGTTTTGAAAGGCGGTCTAGGGCGGATTACTGAAAATATTGCTCCTGGTGATGGAGTCGTCGTGTATTCGATGTCGGACGTGCCTTTGGGGTTTGGAATTGCGGCGAAGTCCACGCAGGACTGTAGGAAATTGGATCCCAATGGGATTGTGGTTCTCCATCAGTCGGATATTGGCGAGTATTTGAGGATGGAAGATGAGCTT

Protein sequence

MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLVKRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNHVLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEYLRMEDEL

Homology

[Close]

BLAST of MS014605.1 vs. NCBI nr
Match: XP_022137772.1 (60S ribosome subunit biogenesis protein NIP7 homolog [Momordica charantia])

HSP 1 Score: 388.7 bits (997), Expect = 2.9e-104
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. NCBI nr
Match: XP_038878449.1 (60S ribosome subunit biogenesis protein NIP7 homolog [Benincasa hispida])

HSP 1 Score: 375.9 bits (964), Expect = 2.0e-100
Identity = 178/187 (95.19%), Postives = 183/187 (97.86%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDE+ETT VFEKLHKFTGNNL+NIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEQETTVVFEKLHKFTGNNLKNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLVALGTCIGK THGGSFHLTV SLGLL+PNAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARSNLVALGTCIGKFTHGGSFHLTVHSLGLLSPNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENI+PGDGVVVYSMSDVPLGFG+AAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENISPGDGVVVYSMSDVPLGFGVAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. NCBI nr
Match: XP_023528444.1 (60S ribosome subunit biogenesis protein NIP7 homolog [Cucurbita pepo subsp. pepo] >XP_023528446.1 60S ribosome subunit biogenesis protein NIP7 homolog [Cucurbita pepo subsp. pepo] >XP_023528447.1 60S ribosome subunit biogenesis protein NIP7 homolog [Cucurbita pepo subsp. pepo] >XP_023528448.1 60S ribosome subunit biogenesis protein NIP7 homolog [Cucurbita pepo subsp. pepo] >XP_023528449.1 60S ribosome subunit biogenesis protein NIP7 homolog [Cucurbita pepo subsp. pepo] >XP_023528450.1 60S ribosome subunit biogenesis protein NIP7 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.8 bits (961), Expect = 4.4e-100
Identity = 180/187 (96.26%), Postives = 182/187 (97.33%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDEKETTAVFEKLHKFTGNNL+NIVENPSHEGP PNPGRYCFRLHKNKVYYVSDSLV
Sbjct: 25  MRPLDEKETTAVFEKLHKFTGNNLKNIVENPSHEGPGPNPGRYCFRLHKNKVYYVSDSLV 84

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLVALGTCIGK THGGSFHLTV SLGLL+ NAKHKVWLKPTSEMSFLYGNH
Sbjct: 85  KRATNVARSNLVALGTCIGKFTHGGSFHLTVHSLGLLSSNAKHKVWLKPTSEMSFLYGNH 144

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 145 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 204

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 205 LRMEDEL 211

[Close]

BLAST of MS014605.1 vs. NCBI nr
Match: XP_004134205.1 (60S ribosome subunit biogenesis protein NIP7 homolog [Cucumis sativus] >KGN57100.1 hypothetical protein Csa_009928 [Cucumis sativus])

HSP 1 Score: 373.6 bits (958), Expect = 9.8e-100
Identity = 178/187 (95.19%), Postives = 182/187 (97.33%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDE+ETT VFEKLHKFTGNNL+NIVENPSHEG EPNPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEQETTVVFEKLHKFTGNNLKNIVENPSHEGTEPNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLV+LGTCIGK THGGSFHLTVQSLGLL+PNAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARSNLVSLGTCIGKFTHGGSFHLTVQSLGLLSPNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENI PGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIVPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. NCBI nr
Match: KAG6582220.1 (60S ribosome subunit biogenesis protein NIP7-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 373.6 bits (958), Expect = 9.8e-100
Identity = 180/187 (96.26%), Postives = 182/187 (97.33%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDEKETTAVFEKLHKFTGNNL+NIVENPSHEGPE NPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEKETTAVFEKLHKFTGNNLKNIVENPSHEGPELNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLVALGTCIGK THGGSFHLTV SLGLL+ NAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARSNLVALGTCIGKFTHGGSFHLTVHSLGLLSSNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. ExPASy Swiss-Prot
Match: A4QND5 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Xenopus tropicalis OX=8364 GN=nip7 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 6.4e-62
Identity = 114/187 (60.96%), Postives = 141/187 (75.40%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPL ++ET A+FEKL K+ G N++ +V+ P         G YCFRLH ++VYYVS+ ++
Sbjct: 1   MRPLTDEETKAMFEKLSKYIGENIKLLVDRPD--------GTYCFRLHNDRVYYVSERIL 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           K ATN+AR  LV+LGTC GK T    F L V +L  LAP AK+KVW+KP +E SFLYGNH
Sbjct: 61  KLATNIARDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWVKPGAEQSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLK GLGRITEN +   GVVVYSM+D+PLGFG+AAKSTQ+CRKLDP  IVV HQ+D+GEY
Sbjct: 121 VLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKSTQECRKLDPMAIVVFHQADVGEY 179

Query: 181 LRMEDEL 188
           +R ED L
Sbjct: 181 IRHEDTL 179

[Close]

BLAST of MS014605.1 vs. ExPASy Swiss-Prot
Match: Q6DFH9 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Xenopus laevis OX=8355 GN=nip7 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-61
Identity = 114/187 (60.96%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPL ++ET A+FEKL K+ G N++ +V+ P         G YCFRLH ++VYYVS+ ++
Sbjct: 1   MRPLTDEETRAMFEKLSKYIGENIKLLVDRPD--------GTYCFRLHNDRVYYVSEKIL 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           K ATN+AR  LV+ GTC GK T    F L V +L  LAP AK+KVW+KP +E SFLYGNH
Sbjct: 61  KLATNIARDKLVSFGTCFGKFTKTQKFRLHVTALDYLAPYAKYKVWVKPGAEQSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLK GLGRITEN +   GVVVYSM+DVPLGFG+AAKSTQ+CRKLDP  IVV HQ+D+GEY
Sbjct: 121 VLKSGLGRITENTSQYQGVVVYSMADVPLGFGVAAKSTQECRKLDPMAIVVFHQADVGEY 179

Query: 181 LRMEDEL 188
           +R ED L
Sbjct: 181 IRHEDTL 179

[Close]

BLAST of MS014605.1 vs. ExPASy Swiss-Prot
Match: Q4T2X8 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Tetraodon nigroviridis OX=99883 GN=nip7 PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 9.3e-61
Identity = 110/187 (58.82%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPL E+ET  +FEKL K+ G N++ +V+ P         G YCFRLH ++VYY+S+ ++
Sbjct: 1   MRPLTEEETKTMFEKLSKYIGENIKLLVDRPD--------GTYCFRLHNDRVYYMSERIL 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           K ATN+AR+ LV++GTC GK T    F L + +L  LAP AK KVW+KP +E SFLYGNH
Sbjct: 61  KLATNIARQKLVSVGTCFGKFTKTSKFRLHITALDFLAPYAKFKVWVKPGAEQSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLK GLGRITEN A   GVVVYSM+DVPLGFG+AAKSTQ+CR++DP  IVV HQ+D+GE+
Sbjct: 121 VLKSGLGRITENTAQYQGVVVYSMADVPLGFGVAAKSTQECRRVDPMSIVVFHQADVGEF 179

Query: 181 LRMEDEL 188
           +R ED L
Sbjct: 181 IRNEDSL 179

[Close]

BLAST of MS014605.1 vs. ExPASy Swiss-Prot
Match: Q9WV50 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Rattus norvegicus OX=10116 GN=Nip7 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 6.0e-60
Identity = 112/187 (59.89%), Postives = 138/187 (73.80%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPL E+ET  +FEK+ K+ G NL+ +V+ P         G YCFRLH ++VYYVS+ ++
Sbjct: 1   MRPLTEEETRVMFEKIAKYIGENLQLLVDRPD--------GTYCFRLHNDRVYYVSEMIL 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           K A N++   LV+LGTC GK T    F L V +L  LAP AK+KVW+KP +E SFLYGNH
Sbjct: 61  KLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDFLAPYAKYKVWIKPGAEQSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLK GLGRITEN +   GVVVYSM+DVPLGFG+AAKSTQDCRK+DP  IVV HQ+DIGEY
Sbjct: 121 VLKSGLGRITENTSQYQGVVVYSMADVPLGFGVAAKSTQDCRKVDPMAIVVFHQADIGEY 179

Query: 181 LRMEDEL 188
           +R E+ L
Sbjct: 181 IRHEETL 179

[Close]

BLAST of MS014605.1 vs. ExPASy Swiss-Prot
Match: Q9Y221 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Homo sapiens OX=9606 GN=NIP7 PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.0e-59
Identity = 111/187 (59.36%), Postives = 138/187 (73.80%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPL E+ET  +FEK+ K+ G NL+ +V+ P         G YCFRLH ++VYYVS+ ++
Sbjct: 1   MRPLTEEETRVMFEKIAKYIGENLQLLVDRPD--------GTYCFRLHNDRVYYVSEKIM 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           K A N++   LV+LGTC GK T    F L V +L  LAP AK+KVW+KP +E SFLYGNH
Sbjct: 61  KLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIKPGAEQSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLK GLGRITEN +   GVVVYSM+D+PLGFG+AAKSTQDCRK+DP  IVV HQ+DIGEY
Sbjct: 121 VLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKSTQDCRKVDPMAIVVFHQADIGEY 179

Query: 181 LRMEDEL 188
           +R E+ L
Sbjct: 181 VRHEETL 179

[Close]

BLAST of MS014605.1 vs. ExPASy TrEMBL
Match: A0A6J1C974 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Momordica charantia OX=3673 GN=LOC111009133 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.4e-104
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. ExPASy TrEMBL
Match: A0A0A0L578 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucumis sativus OX=3659 GN=Csa_3G153740 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 4.7e-100
Identity = 178/187 (95.19%), Postives = 182/187 (97.33%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDE+ETT VFEKLHKFTGNNL+NIVENPSHEG EPNPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEQETTVVFEKLHKFTGNNLKNIVENPSHEGTEPNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLV+LGTCIGK THGGSFHLTVQSLGLL+PNAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARSNLVSLGTCIGKFTHGGSFHLTVQSLGLLSPNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENI PGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIVPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. ExPASy TrEMBL
Match: A0A6J1GYK7 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucurbita moschata OX=3662 GN=LOC111458029 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 6.2e-100
Identity = 180/187 (96.26%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDEKETTAVFEKLHKFTGNNL+NIVENPSHEGPE NPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEKETTAVFEKLHKFTGNNLKNIVENPSHEGPELNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLVALGTCIGK THGGSFHLTV SLGLLA NAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARSNLVALGTCIGKFTHGGSFHLTVHSLGLLASNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIA KSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIATKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. ExPASy TrEMBL
Match: A0A6J1IQ28 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucurbita maxima OX=3661 GN=LOC111479477 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.4e-99
Identity = 179/187 (95.72%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDEKETTAVFEKLHKFTGNNL+NIVENPSHEGPE NPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEKETTAVFEKLHKFTGNNLKNIVENPSHEGPELNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLV LGTCIGK THGGSFHLTV SLGLL+ NAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARSNLVVLGTCIGKFTHGGSFHLTVHSLGLLSSNAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. ExPASy TrEMBL
Match: A0A6J1I8G6 (60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucurbita maxima OX=3661 GN=LOC111472343 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.4e-99
Identity = 177/187 (94.65%), Postives = 182/187 (97.33%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDEKETTAVFEKLHKFTGNNL+NIVENP+HEGPE NPGRYCFRLHKNKVYYVSDSLV
Sbjct: 1   MRPLDEKETTAVFEKLHKFTGNNLKNIVENPAHEGPEQNPGRYCFRLHKNKVYYVSDSLV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATNVAR NLVALGTCIGK THGGSFHLTV SLGLL+ NA+HKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNVARNNLVALGTCIGKFTHGGSFHLTVHSLGLLSANARHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRITENIAPGDGVVVYSMSD+PLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY
Sbjct: 121 VLKGGLGRITENIAPGDGVVVYSMSDIPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180

Query: 181 LRMEDEL 188
           LRMEDEL
Sbjct: 181 LRMEDEL 187

[Close]

BLAST of MS014605.1 vs. TAIR 10
Match: AT4G15770.1 (RNA binding )

HSP 1 Score: 328.2 bits (840), Expect = 4.4e-90
Identity = 154/187 (82.35%), Postives = 169/187 (90.37%), Query Frame = 0


Query: 1   MRPLDEKETTAVFEKLHKFTGNNLRNIVENPSHEGPEPNPGRYCFRLHKNKVYYVSDSLV 60
           MRPLDE ETT VFEKL KF GNNL+ IVENPS EGPE  PG YCFRL KN+VYYVS++LV
Sbjct: 1   MRPLDETETTVVFEKLFKFVGNNLKKIVENPSDEGPESTPGSYCFRLQKNRVYYVSEALV 60

Query: 61  KRATNVARRNLVALGTCIGKITHGGSFHLTVQSLGLLAPNAKHKVWLKPTSEMSFLYGNH 120
           KRATN++R+NLV+ GTCIGK TH GSFHLT+ SL +LA NAKHKVWLKPTSEMSFLYGNH
Sbjct: 61  KRATNISRKNLVSFGTCIGKYTHAGSFHLTIMSLNILAANAKHKVWLKPTSEMSFLYGNH 120

Query: 121 VLKGGLGRITENIAPGDGVVVYSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQSDIGEY 180
           VLKGGLGRIT++I PGDGVVV+SMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQ+DIGEY
Sbjct: 121 VLKGGLGRITDSIVPGDGVVVFSMSDVPLGFGIAAKSTQDCRKLDPNGIVVLHQADIGEY 180

Query: 181 LRMEDEL 188
           LR ED+L
Sbjct: 181 LRGEDDL 187

The following BLAST results are available for this feature:

BLAST of MS014605.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137772.1	2.9e-104	100.00	60S ribosome subunit biogenesis protein NIP7 homolog [Momordica charantia]	[more]
XP_038878449.1	2.0e-100	95.19	60S ribosome subunit biogenesis protein NIP7 homolog [Benincasa hispida]	[more]
XP_023528444.1	4.4e-100	96.26	60S ribosome subunit biogenesis protein NIP7 homolog [Cucurbita pepo subsp. pepo...	[more]
XP_004134205.1	9.8e-100	95.19	60S ribosome subunit biogenesis protein NIP7 homolog [Cucumis sativus] >KGN57100...	[more]
KAG6582220.1	9.8e-100	96.26	60S ribosome subunit biogenesis protein NIP7-like protein, partial [Cucurbita ar...	[more]

BLAST of MS014605.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4QND5	6.4e-62	60.96	60S ribosome subunit biogenesis protein NIP7 homolog OS=Xenopus tropicalis OX=83...	[more]
Q6DFH9	1.1e-61	60.96	60S ribosome subunit biogenesis protein NIP7 homolog OS=Xenopus laevis OX=8355 G...	[more]
Q4T2X8	9.3e-61	58.82	60S ribosome subunit biogenesis protein NIP7 homolog OS=Tetraodon nigroviridis O...	[more]
Q9WV50	6.0e-60	59.89	60S ribosome subunit biogenesis protein NIP7 homolog OS=Rattus norvegicus OX=101...	[more]
Q9Y221	1.0e-59	59.36	60S ribosome subunit biogenesis protein NIP7 homolog OS=Homo sapiens OX=9606 GN=...	[more]

BLAST of MS014605.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C974	1.4e-104	100.00	60S ribosome subunit biogenesis protein NIP7 homolog OS=Momordica charantia OX=3...	[more]
A0A0A0L578	4.7e-100	95.19	60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1GYK7	6.2e-100	96.26	60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucurbita moschata OX=36...	[more]
A0A6J1IQ28	1.4e-99	95.72	60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucurbita maxima OX=3661...	[more]
A0A6J1I8G6	1.4e-99	94.65	60S ribosome subunit biogenesis protein NIP7 homolog OS=Cucurbita maxima OX=3661...	[more]

BLAST of MS014605.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15770.1	4.4e-90	82.35	RNA binding	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002478	PUA domain	SMART	SM00359	pua_5	coord: 103..178 e-value: 3.1E-17 score: 73.3
IPR016686	Ribosome biogenesis factor, NIP7	PIRSF	PIRSF017190	NIP7	coord: 1..187 e-value: 6.3E-78 score: 258.3
None	No IPR available	GENE3D	3.10.450.220		coord: 1..101 e-value: 5.9E-36 score: 124.4
None	No IPR available	PANTHER	PTHR23415:SF4	60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7 HOMOLOG	coord: 1..187
None	No IPR available	PANTHER	PTHR23415	CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7	coord: 1..187
None	No IPR available	PROSITE	PS50890	PUA	coord: 102..178 score: 14.546877
None	No IPR available	SUPERFAMILY	88802	Pre-PUA domain	coord: 1..101
IPR040598	UPF0113, pre-PUA domain	PFAM	PF17833	UPF0113_N	coord: 2..90 e-value: 1.6E-22 score: 79.8
IPR005155	UPF0113, PUA domain	PFAM	PF03657	UPF0113	coord: 104..182 e-value: 1.9E-21 score: 76.1
IPR036974	PUA domain superfamily	GENE3D	2.30.130.10	PUA domain	coord: 102..187 e-value: 1.5E-41 score: 142.2
IPR015947	PUA-like superfamily	SUPERFAMILY	88697	PUA domain-like	coord: 103..178

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS014605	MS014605	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS014605.1-cds	MS014605.1-cds-scaffold1096:31128..31688	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS014605.1	MS014605.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042255	ribosome assembly
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003723	RNA binding