MS014521.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS014521.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionribosomal RNA small subunit methyltransferase I
Locationscaffold553: 579520 .. 583623 (+)
Sequence length1089
RNA-Seq ExpressionMS014521.1
SyntenyMS014521.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGCCAGACGAATTCCTTCCATGGCAGTTCATCTCCCGGAAGTTGTGCCATGGCGTCGTGGTCCTCCCCATTGCATCAATGCGAGGACGGCGCTTGATTCCCACTCATTGGTCTCCTTTTGCCCTAAAACTTGTTTAGTATCCCTTTGTTCTGCTTCTCAATTATCCTCTGAAGTTGCAGATTCAGTTCAGGCTGAAGAATCCTCGAAGCGCGTAAGATCAATAATTTTTAGATTATTTTCCTTCTTTAGAACTGTTACTGTAAGGCTATTGGTTAATTACTCAGAACCCAGAACTCAACCCAAAGCTCAAGTTCTTCTCTCCGTCGTGAACTTGAAAATTAGCACAAACCCATTAGTGATTACGAATATTAATAGAAATGGCATCAGAGAGTTGCTTGGGCTTGGAAATTAGCATGTTTTGTTGTGGCTTCACTTAGACTACTTCTCTTACCGGCACTTCTATTTGATTTAACTACAAGGCTTATAATCTCTGTTGTACTTGGTATCGGTTCAGTATTTACGTTATAGCCGCTTCATAAATGGTAAGATGAAGCCAACATGAGCTTAGCTCAGCGGTAATTGACACCTGTCTCTGACCAAGAGGTCATGAGTTCCAATCCTCCCTTCTCACTTGTTGTACTAAAGAAAAAAATCGTAAGATGAAAATATGAGCTGTATTATATCAGTTTTTCATGGTTAGGATGTGTAGTTTTTGAACTTTTACTGGCATCCTTGCCTTTGTCTCTACGTGTTTCCAGGGTCCCCTGAAACCTGGCTTGTATCTGGTTGGGACGCCAATTGGTAATCTTGAAGATATCACCCTACGGTAATTAAAGTTTGACAACCAGCACAAACATTATTCTCTTTTTTTTTTTTCATAATATATTTAAACCCATAATTTGGAGAATAGACTCTGTTTTGTGCAATTTCTAACTGAGTCATTCCCTCAAGTAGGATGTTAAGTTTGTAAATTTCTGGAACTTTTCCTTTATATTGGTCAATATTTGACGAGTTTGCAACCATGTTTTTTTTGTTCTGCTTATTCTGAATAGTCAATCTTTAAAATTCTGTTATGCAGTGCTATACGTGTCTTAAAATCAGCACACGTCATTCTTTCAGAGGATACAAGGCACTCAGGAAAGTTGCTTCATCATTTCAATATTAGAACCCCCCTTGTAAGTATGCAAAGTCTTTTGTTTACTCATATAATAGTTTTATTCTGCTCCTTCATTTGTTCTGATAAAGAATTTTAGATTTCAGCTGAGCTTTCACAAATTCAATGAGTCTCAAAGAGAGCAATCAGTGTTGAAGAGGTTAAAGCAGGGTGAAATTGTTGCCCTAATTAGTGATGCAGGGACACCAGGCATTAGTGATCCTGGTAGTGAGTTGGTGAGCATGCTAAGCAATTTTAATTCGGTTCTTTCTTGATTGTTCTGTTAAGAATCTATCACTTCTAAGATCTACATTTATTGGGCTCATTTTCAATTTGATTCATTGATAGATTACATTAGCATTTCATAAAAAATTGAAACCGCATGATTGTCCTCCCAGTATTTTAATGGTTTAATCATGGATGATATTGATGTTATTAATACAATTGGACCATGTGAATACAGGTTAAATTATGTGTCAATGAAGACATACCTGTTGTTCCCATTCCTGGGCCCTCCGCTGTGGTTGCTGCTCTTTCTGCCTCTGGCTTGTCAACCGATGAGTTCACATTTGGTAAATTTCTTATCAATTTTCGTAGTTGTGCTGTTAGGACATCTTTCTTTGAGCTATTTGGCTCTATGGCTACTATCTTTAGGCACTCTACAATTTATTAATATTTATATCTGTACAGTTAGATCCCTCTGCCACTAGGCTGTTCTGTTTGCCTCCGGGCTTCTTTTGATTTTGTATACAATCCCATTGTTTCGTATAAAAAAAAAAATATCTGTACAGTTGGATTTGTTCCGAAGCATTCTGCATCCAGAAGAGAGAGGCTGACGGTTTCAGCAAATGAGGAAGCAACTCAAATATTCTATGTTCCCCCTCATAAACTTAAGCAGTTTGTCGAGGAAACTTTATCATTATTTGGTGAATCAAGGTACTTTGTATATGTCTATATCCAATCTATTGTACTAATATTTCTTTCAAAGTCTAATGTATCAGTGCAGTTCCACTTTCGCTTCTGCTTCAATCTACATAATTCAGTTACTAATGACATTCTTCGTATATGTTACATTGTATAACTATAGCAGCTCGTTTGTGTGTTTCTTTCCAGTAACTATGTTCTATAATATTGTACATGTGCTTTTAGATTCAGATAGTCTGAAACTATTTCTTTAATGGAGGATCAGACTTGTCTTCTGTTAGTAGTTGGTTTGCTTATTTGCCAACTTAGTGATTTTATCTGTTTCTATAATGGTCTTGCAGACGATGTGTTATAGCTCGTGAAATGACAAAGATACATGAGGAGGTAACTTCACTCTGTCGCTCTGTTTTGGTATCTTTAGTTGGCAGGATTTCCAATACCCTTGCTAACTGGCGAGCATCATATTCCATTCATCAAAAAATTTCTTCTGTGCACTGTTTCGTAATGCCCTTATATGATATCGTTCAACCAATTTCTTATGTGCTCTTTTGAGTTCCTTCAATTTTTAATTCTCAAAATATGATTTTATAAGTTTTAAGCATCAAATAATAGTAAAAGTAAATAATTATGTTATTTGGTGGAGTAACAAGATATTACCTAAATGGCTGCTAAACGTGTATTTACGTACAGTAACTAGATGCTTTGGAACAGGCTGCTCACCGTAAATGTGATTGGTTGTAATTGCAACTGCAAAAGTTGATTTTCGTGTATTTGGGTATGAAAATCAATAACTGCCTGAAGGTTGTTTGTAATATGTTATTCCTAATCTCTTTGTTTCACCTGTTATATGAACCTAGGCTTCCTCTCTCCTTGTTGGTACTCTAATTACTTTAAATAGAAAATACTTTAGTTTCTCATCCAAAAGCTAGAAAGCGTACGTTGAATTTTCAATTTCATCATTATTGTGAAATATATTGTCATAATATTTTTCTTTTGGAAGATTTGATTGCTGCACTTGGGGGAGTTGGGTCCATACTGTCATTTAGAACATGTATGATGAATGGCCTTATATTTGTAGTTGATTTTTTCTCCAATCTACTTTCTTTCAATCATTAAACTACCATTATGTGACGTCGTTTGTGGTGGATACATAATTGTAATAATTATAGATATCCTTGAATAATGTAATTTGTAATAACTCAAATTATGCATTTCACATCCTCGTTGTGTTACTTAGTAACAAGTATAATGATGTCAACAGTTTTGGCGGGGCACACTGGCTGAAGCTAAAGAGTTTTTCTCGTTGCACCAAACAAAGGGCGAAATTACGCTGTTGATTGAAGGAAAGCTAGATTCTGGAGTTGAAGCTCCATCAGAATTGCAATTGGAAAAGGAGTTGCAAGAATTGATCTCTAGTGGCCATAGGCTTTCTATGGTAAGGGCTTAAACTGGACTATAGAATAATAGAGAATTTTATGAGCGGTAATCCCTTTTAGAGGCTCGGTAGATCTTATCATTAAAGGAAAGCATAAGCTAAACCTAATGGACAAAATGAGTGTTTTACCGTAAGTAAATATTGGTTTAAACCTCATTCTGGTCCTTAACTTTTAAATTTACTTTACATGTAGGGTAGTTCTTCAACTTTGTTCTCGCTATTATTTTGCTGTTAATTATTCAATAGATGGATGGAGTGAGTTAGAATATGCGTGGTAATATACTGATGTGAATATGTATTGAAGGCATATAGGCTAACTAAGGTGGGTGTTTGAGTCAAATATTAGGTGGTAAAGACAAAAGTTCTTACTTTGTTAAAATTTTGAGACTAAAAAGAGATGTTTTGGAAATTCAAGATCAAAATAAGGTTTAAACTCTGCGCTGAATTCTTGGTTGTGATGAAACTTTATTTGGATGAAGGCTGTCAAATTGGTTGCTAGCAAAACATCAGCAAGTAGGAAAACCGTCTATTCGATTGCATTGCGAAAATTCGGGAATCAACTTGGAGCGGACAGCGATTCTTGTAAA

mRNA sequence

ATGTTGGCCAGACGAATTCCTTCCATGGCAGTTCATCTCCCGGAAGTTGTGCCATGGCGTCGTGGTCCTCCCCATTGCATCAATGCGAGGACGGCGCTTGATTCCCACTCATTGGTCTCCTTTTGCCCTAAAACTTGTTTAGTATCCCTTTGTTCTGCTTCTCAATTATCCTCTGAAGTTGCAGATTCAGTTCAGGCTGAAGAATCCTCGAAGCGCGGTCCCCTGAAACCTGGCTTGTATCTGGTTGGGACGCCAATTGGTAATCTTGAAGATATCACCCTACGTGCTATACGTGTCTTAAAATCAGCACACGTCATTCTTTCAGAGGATACAAGGCACTCAGGAAAGTTGCTTCATCATTTCAATATTAGAACCCCCCTTCTGAGCTTTCACAAATTCAATGAGTCTCAAAGAGAGCAATCAGTGTTGAAGAGGTTAAAGCAGGGTGAAATTGTTGCCCTAATTAGTGATGCAGGGACACCAGGCATTAGTGATCCTGGTAGTGAGTTGGTTAAATTATGTGTCAATGAAGACATACCTGTTGTTCCCATTCCTGGGCCCTCCGCTGTGGTTGCTGCTCTTTCTGCCTCTGGCTTGTCAACCGATGAGTTCACATTTGTTGGATTTGTTCCGAAGCATTCTGCATCCAGAAGAGAGAGGCTGACGGTTTCAGCAAATGAGGAAGCAACTCAAATATTCTATGTTCCCCCTCATAAACTTAAGCAGTTTGTCGAGGAAACTTTATCATTATTTGGTGAATCAAGACGATGTGTTATAGCTCGTGAAATGACAAAGATACATGAGGAGTTTTGGCGGGGCACACTGGCTGAAGCTAAAGAGTTTTTCTCGTTGCACCAAACAAAGGGCGAAATTACGCTGTTGATTGAAGGAAAGCTAGATTCTGGAGTTGAAGCTCCATCAGAATTGCAATTGGAAAAGGAGTTGCAAGAATTGATCTCTAGTGGCCATAGGCTTTCTATGGCTGTCAAATTGGTTGCTAGCAAAACATCAGCAAGTAGGAAAACCGTCTATTCGATTGCATTGCGAAAATTCGGGAATCAACTTGGAGCGGACAGCGATTCTTGTAAA

Coding sequence (CDS)

ATGTTGGCCAGACGAATTCCTTCCATGGCAGTTCATCTCCCGGAAGTTGTGCCATGGCGTCGTGGTCCTCCCCATTGCATCAATGCGAGGACGGCGCTTGATTCCCACTCATTGGTCTCCTTTTGCCCTAAAACTTGTTTAGTATCCCTTTGTTCTGCTTCTCAATTATCCTCTGAAGTTGCAGATTCAGTTCAGGCTGAAGAATCCTCGAAGCGCGGTCCCCTGAAACCTGGCTTGTATCTGGTTGGGACGCCAATTGGTAATCTTGAAGATATCACCCTACGTGCTATACGTGTCTTAAAATCAGCACACGTCATTCTTTCAGAGGATACAAGGCACTCAGGAAAGTTGCTTCATCATTTCAATATTAGAACCCCCCTTCTGAGCTTTCACAAATTCAATGAGTCTCAAAGAGAGCAATCAGTGTTGAAGAGGTTAAAGCAGGGTGAAATTGTTGCCCTAATTAGTGATGCAGGGACACCAGGCATTAGTGATCCTGGTAGTGAGTTGGTTAAATTATGTGTCAATGAAGACATACCTGTTGTTCCCATTCCTGGGCCCTCCGCTGTGGTTGCTGCTCTTTCTGCCTCTGGCTTGTCAACCGATGAGTTCACATTTGTTGGATTTGTTCCGAAGCATTCTGCATCCAGAAGAGAGAGGCTGACGGTTTCAGCAAATGAGGAAGCAACTCAAATATTCTATGTTCCCCCTCATAAACTTAAGCAGTTTGTCGAGGAAACTTTATCATTATTTGGTGAATCAAGACGATGTGTTATAGCTCGTGAAATGACAAAGATACATGAGGAGTTTTGGCGGGGCACACTGGCTGAAGCTAAAGAGTTTTTCTCGTTGCACCAAACAAAGGGCGAAATTACGCTGTTGATTGAAGGAAAGCTAGATTCTGGAGTTGAAGCTCCATCAGAATTGCAATTGGAAAAGGAGTTGCAAGAATTGATCTCTAGTGGCCATAGGCTTTCTATGGCTGTCAAATTGGTTGCTAGCAAAACATCAGCAAGTAGGAAAACCGTCTATTCGATTGCATTGCGAAAATTCGGGAATCAACTTGGAGCGGACAGCGATTCTTGTAAA

Protein sequence

MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEVADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHHFNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIPVVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKLKQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLDSGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSDSCK
Homology
BLAST of MS014521.1 vs. NCBI nr
Match: XP_022144419.1 (uncharacterized protein LOC111014109 [Momordica charantia])

HSP 1 Score: 691.8 bits (1784), Expect = 3.1e-195
Identity = 358/363 (98.62%), Postives = 359/363 (98.90%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           MLARRIPSMAVHLPEVVPWRRGP HCINARTALDSH   SFCPKTCLVSLCSASQLSSEV
Sbjct: 1   MLARRIPSMAVHLPEVVPWRRGPSHCINARTALDSH---SFCPKTCLVSLCSASQLSSEV 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
           ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH
Sbjct: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP
Sbjct: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL
Sbjct: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD
Sbjct: 241 KQFLEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD
Sbjct: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 360

BLAST of MS014521.1 vs. NCBI nr
Match: XP_023538391.1 (uncharacterized protein LOC111799188 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.4 bits (1583), Expect = 6.4e-172
Identity = 308/363 (84.85%), Postives = 340/363 (93.66%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           ML RRIPS+AV+LP+ +PWRRGPPH I ART L+ HSL+SFCPKTCL+S CSASQ SSEV
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGPPHSIKARTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
           A+    ++SSKRGPLKPGLY+V TPIGNLEDITLRA+RVLKSA+VILSEDTRHSGKLL H
Sbjct: 61  AEPTHMDDSSKRGPLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           F+I+TPLLSFHKFNESQREQ+VLKRLK+GEIVALISDAGTPGISDPG+ELV+ CVN  IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRQCVNASIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           VVPIPGPSAV+AALSASGLSTDEFTFVGFVPKH++SRRERL VSANEEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTDEFTFVGFVPKHASSRRERLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EET SLFGESR+CVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEGKL+
Sbjct: 241 KQFLEETSSLFGESRQCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           S VE PSE QLEKEL+ELISSGH+LSMAVKLVA KTSASRKTVYSIALR+FGNQLGA++D
Sbjct: 301 SEVETPSESQLEKELEELISSGHKLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGAEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

BLAST of MS014521.1 vs. NCBI nr
Match: XP_022951884.1 (uncharacterized protein LOC111454620 [Cucurbita moschata])

HSP 1 Score: 613.6 bits (1581), Expect = 1.1e-171
Identity = 309/363 (85.12%), Postives = 339/363 (93.39%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           ML RRIPS+AV+LP+ +PWRRG PH IN RT L+ HSL+SFCPKTCL+S CSASQ SSEV
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGLPHSINTRTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
           A+    ++SSKRGPLKPGLY+V TPIGNLEDITLRA+RVLKSA+VILSEDTRHSGKLL H
Sbjct: 61  AEPTHLDDSSKRGPLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           F+I+TPLLSFHKFNESQREQ+VLKRLK+GEIVALISDAGTPGISDPG+ELV+ CVN  IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRQCVNASIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           VVPIPGPSAV+AALSASGLSTDEFTFVGFVPKH+ASRRERL VSANEEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTDEFTFVGFVPKHAASRRERLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EET SLFGESR+CVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEGKL+
Sbjct: 241 KQFLEETSSLFGESRQCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           S VE PSE QLEKEL+ELISSGHRLSMAVKLVA KTSASRKTVYSIALR+FGNQLGA++D
Sbjct: 301 SEVETPSESQLEKELEELISSGHRLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGAEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

BLAST of MS014521.1 vs. NCBI nr
Match: XP_023002398.1 (uncharacterized protein LOC111496252 [Cucurbita maxima])

HSP 1 Score: 610.1 bits (1572), Expect = 1.2e-170
Identity = 306/363 (84.30%), Postives = 338/363 (93.11%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           ML RRIPS+AV+LP+ +PWRRGPPH IN RT L+ HSL+SFCPKTCL+S CSASQ SSEV
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINTRTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
           A+    ++SSKRG LKPGLY+V TPIGNLEDITLRA+RVLKSA+VILSEDTRHSGKLL H
Sbjct: 61  AEPTHMDDSSKRGSLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           F+I+TPLLSFHKFNESQREQ+VLKRLK+GEIVALISDAGTPGISDPG+ELV+LCVN  IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRLCVNASIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           VVPIPGPSAV+AALSASGLST+EFTFVGFVPKH+ASRR+RL VSANEEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTNEFTFVGFVPKHAASRRKRLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EET SLFGESR+CVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEGKL+
Sbjct: 241 KQFLEETSSLFGESRKCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           S VE PSE QLE EL+ELISSGHRLSMAVKLVA KTSASRKTVYSIALR+FGNQLG ++D
Sbjct: 301 SEVETPSESQLENELEELISSGHRLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGTEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

BLAST of MS014521.1 vs. NCBI nr
Match: XP_038886193.1 (ribosomal RNA small subunit methyltransferase I [Benincasa hispida])

HSP 1 Score: 609.4 bits (1570), Expect = 2.1e-170
Identity = 305/363 (84.02%), Postives = 336/363 (92.56%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           ML RRIPS+AV+LP+ +PWRRGPPH IN RT L+ HS ++FCPK+C VS CSASQ  SE 
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINPRTPLEFHSFLAFCPKSCFVSFCSASQSISEA 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
            + + AEES KR PLKPGLYLVGTPIGNLEDITLRA+RVLKSAHVILSEDTRHSGKLL H
Sbjct: 61  VEPIHAEESLKREPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLLQH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           F+IRTPLLSFHKFNESQREQ+V+KRLK+GEIVALISDAGTPGISDPG+ELV+LCVN++IP
Sbjct: 121 FSIRTPLLSFHKFNESQREQTVVKRLKEGEIVALISDAGTPGISDPGTELVRLCVNKNIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           V+P+PGPSAVVAA+SASGLSTDEFTFVGFVPKH+ASRRERL VSA+EEATQIFYVPPHKL
Sbjct: 181 VIPVPGPSAVVAAISASGLSTDEFTFVGFVPKHAASRRERLMVSASEEATQIFYVPPHKL 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EET  LFGESR+CVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEG L+
Sbjct: 241 KQFLEETSQLFGESRQCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGNLN 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           S VE PSE QLEKEL+ELISSGHRLS AVKLVASKTSA RKTVYSIALR+FGNQLGA++D
Sbjct: 301 SEVETPSESQLEKELEELISSGHRLSTAVKLVASKTSARRKTVYSIALRRFGNQLGAEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

BLAST of MS014521.1 vs. ExPASy Swiss-Prot
Match: P74038 (Ribosomal RNA small subunit methyltransferase I OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=rsmI PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.7e-61
Identity = 123/270 (45.56%), Postives = 172/270 (63.70%), Query Frame = 0

Query: 79  LYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHHFNIRTPLLSFHKFNESQR 138
           LYLV TPIGNL D+T RA+  L++  +I +EDTRH+GKLL HF I TP +S+H  N   R
Sbjct: 4   LYLVATPIGNLGDMTPRAVETLQTVDLIAAEDTRHTGKLLQHFQITTPQISYHDHNRHGR 63

Query: 139 EQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIPVVPIPGPSAVVAALSASG 198
            Q +L +L+ G+ +AL+SDAGTPGISDPG ELV  C   +I V+PIPG +A++AAL +SG
Sbjct: 64  TQELLAKLQAGQNIALVSDAGTPGISDPGQELVAACGEANIEVIPIPGATALIAALISSG 123

Query: 199 LSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKLKQFVEETLSLFGESRRCV 258
           L+TD F F GF+   +  R++ L   A EE T I Y  PH+L   + +  +  G+ R   
Sbjct: 124 LATDRFVFEGFLSTKNRPRQQLLQSLAQEERTIILYEAPHRLLATLTDLQTFLGQERSLT 183

Query: 259 IAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLDSGVEAPSELQLEKELQEL 318
           +ARE+TK HE+FWRGTL  A  +F+ +  KGE  L+I G       + SE  L  EL+ L
Sbjct: 184 VARELTKYHEQFWRGTLQTAIAYFTENTPKGEFCLVIAGATPEDRPSFSEENLRDELRSL 243

Query: 319 ISSGHRLSMAVKLVASKTSASRKTVYSIAL 349
           ++ G   S A + +A +T   R+ +Y ++L
Sbjct: 244 MAKGLTRSQASRQLAEETKLPRRQLYQLSL 273

BLAST of MS014521.1 vs. ExPASy Swiss-Prot
Match: Q9KGL2 (Ribosomal RNA small subunit methyltransferase I OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=rsmI PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.0e-55
Identity = 125/288 (43.40%), Postives = 179/288 (62.15%), Query Frame = 0

Query: 64  VQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHHFNI 123
           ++ ++S ++   K  LYLV TPIGNLED+T RAIR LK A  I +EDTR + KLL+HF+I
Sbjct: 1   MKTQQSYQQRDDKGTLYLVATPIGNLEDVTFRAIRTLKEADQIAAEDTRQTKKLLNHFDI 60

Query: 124 RTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIPVVP 183
            T L+S+H+ N+    + ++  L +G  +AL+SDAG P ISDPG ELV   + E I V+P
Sbjct: 61  ATKLVSYHEHNKETMGKRLIDDLIEGRTIALVSDAGMPAISDPGYELVVSAIKEGIAVIP 120

Query: 184 IPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKLKQF 243
           IPG +A V AL ASGL T+ F F+GF+P+    RR+ L  +   +AT IFY  PH+LK  
Sbjct: 121 IPGANAAVTALIASGLPTESFQFIGFLPRQKKQRRQALEETKPTKATLIFYESPHRLKDT 180

Query: 244 VEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLDSGV 303
           +++ L + G +R   I RE+TK +EEF RGTL EA  +      KGE  L++EG  +  V
Sbjct: 181 LDDMLLILG-NRHVSICRELTKTYEEFLRGTLEEAVHWAREATIKGEFCLIVEGNGEK-V 240

Query: 304 EAPSELQLE-----KELQELISSGHRLSMAVKLVASKTSASRKTVYSI 347
           E P E+  E     + ++  I+ G R   A+K VA+     ++ +Y+I
Sbjct: 241 E-PEEVWWESLSPVQHVEHYIALGFRSKEAIKQVATDRGVPKRDIYNI 285

BLAST of MS014521.1 vs. ExPASy Swiss-Prot
Match: P45298 (Ribosomal RNA small subunit methyltransferase I OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=rsmI PE=3 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.8e-51
Identity = 114/272 (41.91%), Postives = 160/272 (58.82%), Query Frame = 0

Query: 79  LYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHHFNIRTPLLSFHKFNESQR 138
           LY+V TPIGNL+DIT RA+       +I +EDTRHSG LL H+ I+ P  + H  NE ++
Sbjct: 8   LYIVATPIGNLQDITQRALETFAQVDLIAAEDTRHSGLLLSHYGIKKPFFALHDHNEQEK 67

Query: 139 EQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIPVVPIPGPSAVVAALSASG 198
              ++++LKQG  +ALISDAGTP ISDPG  LV+ C    I VVP+PG  A + AL ASG
Sbjct: 68  AHILVEKLKQGSNIALISDAGTPLISDPGFHLVRQCREAGIRVVPLPGACAAITALCASG 127

Query: 199 LSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKLKQFVEETLSLFGESRRCV 258
           +++D F F GF+P  S +R+++L   A E+ T IFY   H++   +E+  ++ GE R  V
Sbjct: 128 IASDRFCFEGFLPAKSKARKDKLENIAEEDRTLIFYESTHRILDTLEDMQAVLGEERYIV 187

Query: 259 IAREMTKIHEEFWRGTLAEAKEFF--SLHQTKGEITLLIEGKLDSGVEAPSELQLEKELQ 318
           +AREMTK  E     T+   +E+     ++TKGE+ L++EGK  S        Q  K L 
Sbjct: 188 LAREMTKTWETITGNTIKNLREWLLEDPNRTKGEMVLIVEGKPKSDNNDEISPQAVKAL- 247

Query: 319 ELISSGHRLSMAVKLVASKTSASRKTVYSIAL 349
           ELI+    L  A  +VA      +  +Y   L
Sbjct: 248 ELIAEELPLKKAAAIVAELYGYKKNALYQFGL 278

BLAST of MS014521.1 vs. ExPASy Swiss-Prot
Match: Q87B70 (Ribosomal RNA small subunit methyltransferase I OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) OX=183190 GN=rsmI PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.9e-50
Identity = 116/271 (42.80%), Postives = 164/271 (60.52%), Query Frame = 0

Query: 77  PG-LYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHHFNIRTPLLSFHKFNE 136
           PG L++V TPIGNL D++ RA  +L++  +I +EDTRH+ +LL HF I  PLL+ H  NE
Sbjct: 4   PGTLHIVATPIGNLGDLSPRAQHILRTVTMICAEDTRHTRQLLAHFGIERPLLALHAHNE 63

Query: 137 SQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIPVVPIPGPSAVVAALS 196
               + ++ RL  GE +AL+SDAGTP ISDPG  LV+      I V P+PGPSA++AALS
Sbjct: 64  DTLAERIITRLISGESLALVSDAGTPLISDPGFLLVRAARAAGIRVTPVPGPSALIAALS 123

Query: 197 ASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKLKQFVEETLSLFGESR 256
            SGL ++ FTF GF+P    +RR+RLT  A+E  T IFY   H++ + + +  ++FG  R
Sbjct: 124 VSGLPSNRFTFEGFLPAKPTARRDRLTHLAHEPRTLIFYEASHRIAECLTDLATIFGSMR 183

Query: 257 RCVIAREMTKIHEEFWRGTLA--EAKEFFSLHQTKGEITLLIEGKLDSGVEAPSELQLEK 316
             V+ARE+TKI E    GTLA  + +     +Q KGE  ++++G  D      +E    +
Sbjct: 184 IAVVARELTKIFETVLDGTLATLQTQVANDENQRKGEFVIIVQGAADQDAAKITE---GR 243

Query: 317 ELQELISSGHRLSMAVKLVASKTSASRKTVY 345
            +  L+      S A KL A  T A RK +Y
Sbjct: 244 RIYALLKEHLPPSSAAKLAAEITGAPRKALY 271

BLAST of MS014521.1 vs. ExPASy Swiss-Prot
Match: Q9KUD9 (Ribosomal RNA small subunit methyltransferase I OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=rsmI PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.4e-50
Identity = 120/281 (42.70%), Postives = 162/281 (57.65%), Query Frame = 0

Query: 77  PGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHHFNIRTPLLSFHKFNES 136
           P LY+V TPIGNL DIT RA+ VL S  +I  EDTRH+GKLL HFNI T   + H  NE 
Sbjct: 12  PILYIVPTPIGNLGDITQRALDVLASVDMIAVEDTRHTGKLLAHFNISTKTFALHDHNEQ 71

Query: 137 QREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIPVVPIPGPSAVVAALSA 196
           Q+ Q ++ +L  G  +AL+SDAGTP ISDPG  LV  C    + VVP+PGP AV+ ALSA
Sbjct: 72  QKAQVLVDKLLSGLSIALVSDAGTPLISDPGYHLVTQCRQAGVKVVPLPGPCAVITALSA 131

Query: 197 SGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKLKQFVEETLSLFGESRR 256
           SGL +D F+F GF+P  S +R+++L   A    T IFY  PH++ + +++ L + G  R 
Sbjct: 132 SGLPSDSFSFEGFLPAKSKARKDKLLEIAKVSRTCIFYESPHRICESLQDMLDVLGGERE 191

Query: 257 CVIAREMTKIHEEFWRGTLAEAKEFFSL--HQTKGEITLLIEGKLDSGVEAPSELQLEKE 316
            V+ARE+TK  E      LAE   + +   ++ KGE+ LL+ G  D+G     E QL  E
Sbjct: 192 VVLARELTKTFETIQGMPLAELITWIAEDDNRKKGEMVLLVHGYRDAG-----EQQLPDE 251

Query: 317 LQE---LISSGHRLSMAVKLVASKTSASRKTVYSIALRKFG 353
                 +++    L  A  LVA      +  +Y   L   G
Sbjct: 252 ALRTLTILTKELPLKKAAALVAEIHQLKKNALYKWGLENLG 287

BLAST of MS014521.1 vs. ExPASy TrEMBL
Match: A0A6J1CS92 (uncharacterized protein LOC111014109 OS=Momordica charantia OX=3673 GN=LOC111014109 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.5e-195
Identity = 358/363 (98.62%), Postives = 359/363 (98.90%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           MLARRIPSMAVHLPEVVPWRRGP HCINARTALDSH   SFCPKTCLVSLCSASQLSSEV
Sbjct: 1   MLARRIPSMAVHLPEVVPWRRGPSHCINARTALDSH---SFCPKTCLVSLCSASQLSSEV 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
           ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH
Sbjct: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP
Sbjct: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL
Sbjct: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD
Sbjct: 241 KQFLEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD
Sbjct: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 360

BLAST of MS014521.1 vs. ExPASy TrEMBL
Match: A0A6J1GK22 (uncharacterized protein LOC111454620 OS=Cucurbita moschata OX=3662 GN=LOC111454620 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.3e-172
Identity = 309/363 (85.12%), Postives = 339/363 (93.39%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           ML RRIPS+AV+LP+ +PWRRG PH IN RT L+ HSL+SFCPKTCL+S CSASQ SSEV
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGLPHSINTRTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
           A+    ++SSKRGPLKPGLY+V TPIGNLEDITLRA+RVLKSA+VILSEDTRHSGKLL H
Sbjct: 61  AEPTHLDDSSKRGPLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           F+I+TPLLSFHKFNESQREQ+VLKRLK+GEIVALISDAGTPGISDPG+ELV+ CVN  IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRQCVNASIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           VVPIPGPSAV+AALSASGLSTDEFTFVGFVPKH+ASRRERL VSANEEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTDEFTFVGFVPKHAASRRERLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EET SLFGESR+CVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEGKL+
Sbjct: 241 KQFLEETSSLFGESRQCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           S VE PSE QLEKEL+ELISSGHRLSMAVKLVA KTSASRKTVYSIALR+FGNQLGA++D
Sbjct: 301 SEVETPSESQLEKELEELISSGHRLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGAEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

BLAST of MS014521.1 vs. ExPASy TrEMBL
Match: A0A6J1KTF7 (uncharacterized protein LOC111496252 OS=Cucurbita maxima OX=3661 GN=LOC111496252 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.8e-171
Identity = 306/363 (84.30%), Postives = 338/363 (93.11%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPHCINARTALDSHSLVSFCPKTCLVSLCSASQLSSEV 60
           ML RRIPS+AV+LP+ +PWRRGPPH IN RT L+ HSL+SFCPKTCL+S CSASQ SSEV
Sbjct: 1   MLTRRIPSVAVYLPQALPWRRGPPHSINTRTPLEFHSLLSFCPKTCLLSFCSASQFSSEV 60

Query: 61  ADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLLHH 120
           A+    ++SSKRG LKPGLY+V TPIGNLEDITLRA+RVLKSA+VILSEDTRHSGKLL H
Sbjct: 61  AEPTHMDDSSKRGSLKPGLYIVSTPIGNLEDITLRALRVLKSANVILSEDTRHSGKLLQH 120

Query: 121 FNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNEDIP 180
           F+I+TPLLSFHKFNESQREQ+VLKRLK+GEIVALISDAGTPGISDPG+ELV+LCVN  IP
Sbjct: 121 FSIKTPLLSFHKFNESQREQTVLKRLKEGEIVALISDAGTPGISDPGTELVRLCVNASIP 180

Query: 181 VVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPHKL 240
           VVPIPGPSAV+AALSASGLST+EFTFVGFVPKH+ASRR+RL VSANEEATQIFYVPPHK+
Sbjct: 181 VVPIPGPSAVIAALSASGLSTNEFTFVGFVPKHAASRRKRLMVSANEEATQIFYVPPHKV 240

Query: 241 KQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGKLD 300
           KQF+EET SLFGESR+CVIAREMTKIHEEFWRGTLAEAKE+FSLHQTKGEITLLIEGKL+
Sbjct: 241 KQFLEETSSLFGESRKCVIAREMTKIHEEFWRGTLAEAKEYFSLHQTKGEITLLIEGKLN 300

Query: 301 SGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGADSD 360
           S VE PSE QLE EL+ELISSGHRLSMAVKLVA KTSASRKTVYSIALR+FGNQLG ++D
Sbjct: 301 SEVETPSESQLENELEELISSGHRLSMAVKLVAIKTSASRKTVYSIALRRFGNQLGTEND 360

Query: 361 SCK 364
           SCK
Sbjct: 361 SCK 363

BLAST of MS014521.1 vs. ExPASy TrEMBL
Match: A0A1S3BBU6 (ribosomal RNA small subunit methyltransferase I OS=Cucumis melo OX=3656 GN=LOC103488001 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.7e-170
Identity = 309/365 (84.66%), Postives = 341/365 (93.42%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPP--HCINARTALDSHSLVSFCPKTCLVSLCSASQLSS 60
           MLARR+PS+AV+LP+ +PWRR PP  H IN+RTAL  HSL++FCPKT LVSLCSASQL++
Sbjct: 1   MLARRMPSVAVYLPQALPWRRRPPHSHSINSRTALVFHSLLAFCPKTILVSLCSASQLAT 60

Query: 61  EVADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLL 120
           E  + + AEE SKRGPLKPGLYLVGTPIGNLEDITLRA+RVLKSAHVILSEDTRHSGKLL
Sbjct: 61  EAVEPIHAEEFSKRGPLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120

Query: 121 HHFNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNED 180
            HF+I+TPLLSFHKFNESQREQ+VLKRLKQGEIVALISDAGTPGISDPG+ELV+LCVNE+
Sbjct: 121 QHFSIKTPLLSFHKFNESQREQTVLKRLKQGEIVALISDAGTPGISDPGTELVRLCVNEN 180

Query: 181 IPVVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPH 240
           IPVVP+PGPSAVVAA+SASGLSTDEFTFVGF+PKH+A+RRERL VSANEEATQIFYVPPH
Sbjct: 181 IPVVPVPGPSAVVAAISASGLSTDEFTFVGFLPKHAATRRERLMVSANEEATQIFYVPPH 240

Query: 241 KLKQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGK 300
           KLKQF+EET  LFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSL+ TKGEITLLIEGK
Sbjct: 241 KLKQFLEETSQLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLNPTKGEITLLIEGK 300

Query: 301 LDSGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGAD 360
           L   VE PSE QLEKEL+ELIS+GHRLSMAVKLVASKTSASRKTVYSIALR+FGNQLG +
Sbjct: 301 LSPEVETPSESQLEKELEELISTGHRLSMAVKLVASKTSASRKTVYSIALRRFGNQLGVE 360

Query: 361 SDSCK 364
           ++SCK
Sbjct: 361 NNSCK 365

BLAST of MS014521.1 vs. ExPASy TrEMBL
Match: A0A0A0LRJ7 (TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G362440 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.7e-170
Identity = 311/365 (85.21%), Postives = 339/365 (92.88%), Query Frame = 0

Query: 1   MLARRIPSMAVHLPEVVPWRRGPPH--CINARTALDSHSLVSFCPKTCLVSLCSASQLSS 60
           MLARRIPS+AV+LP+ +PWRR PPH   IN  TAL+ HSL++FCPKT LVSLCSASQL+ 
Sbjct: 1   MLARRIPSVAVYLPQALPWRRRPPHSYSINYGTALEFHSLLAFCPKTLLVSLCSASQLTY 60

Query: 61  EVADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAHVILSEDTRHSGKLL 120
           E  + + AEESSKRG LKPGLYLVGTPIGNLEDITLRA+RVLKSAHVILSEDTRHSGKLL
Sbjct: 61  EAVEPIHAEESSKRGSLKPGLYLVGTPIGNLEDITLRALRVLKSAHVILSEDTRHSGKLL 120

Query: 121 HHFNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGISDPGSELVKLCVNED 180
            HF+I+TPLLSFHKFNESQREQ+VLKRLKQGEIVALISDAGTPGISDPG+ELV+LCVNE+
Sbjct: 121 QHFSIKTPLLSFHKFNESQREQTVLKRLKQGEIVALISDAGTPGISDPGTELVRLCVNEN 180

Query: 181 IPVVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVSANEEATQIFYVPPH 240
           IPVVP+PGPSAVVAA+SASGLSTDEFTFVGF+PKH+ASRRERL VSANEEATQIFYVPPH
Sbjct: 181 IPVVPVPGPSAVVAAISASGLSTDEFTFVGFLPKHAASRRERLMVSANEEATQIFYVPPH 240

Query: 241 KLKQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLHQTKGEITLLIEGK 300
           KLKQF+EET  LFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSL+QTKGEITLLIEGK
Sbjct: 241 KLKQFLEETSQLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSLNQTKGEITLLIEGK 300

Query: 301 LDSGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVYSIALRKFGNQLGAD 360
           L   VE PSE QLEKEL+ELIS+GHRLSMAVKLVASKTS SRKTVYSIALR+FGNQLG +
Sbjct: 301 LCPEVETPSESQLEKELEELISTGHRLSMAVKLVASKTSTSRKTVYSIALRRFGNQLGVE 360

Query: 361 SDSCK 364
           +DSCK
Sbjct: 361 NDSCK 365

BLAST of MS014521.1 vs. TAIR 10
Match: AT1G45110.1 (Tetrapyrrole (Corrin/Porphyrin) Methylases )

HSP 1 Score: 435.6 bits (1119), Expect = 3.8e-122
Identity = 225/316 (71.20%), Postives = 264/316 (83.54%), Query Frame = 0

Query: 45  TCLVSLCSASQLSSEVADSVQAEESSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLKSAH 104
           T  +S CS    SSE ADSV A++ SKRGPLKPGLYLVGTPIGNLEDITLRAIRVL+SA 
Sbjct: 28  TAAISFCS----SSEFADSV-AKDDSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLRSAD 87

Query: 105 VILSEDTRHSGKLLHHFNIRTPLLSFHKFNESQREQSVLKRLKQGEIVALISDAGTPGIS 164
           VILSEDTRHSGKLL ++NI+  LLS+HKFNE+QREQ+VL RLKQGEIVALISDAGTPGIS
Sbjct: 88  VILSEDTRHSGKLLQYYNIKAQLLSYHKFNEAQREQAVLTRLKQGEIVALISDAGTPGIS 147

Query: 165 DPGSELVKLCVNEDIPVVPIPGPSAVVAALSASGLSTDEFTFVGFVPKHSASRRERLTVS 224
           DPG++L K+C  E+I V+PIPG  AVVAALSASGL TDEFTFVGF+PKHS +R+ERL VS
Sbjct: 148 DPGTQLAKMCAKENIDVIPIPGACAVVAALSASGLETDEFTFVGFLPKHSGTRKERLIVS 207

Query: 225 ANEEATQIFYVPPHKLKQFVEETLSLFGESRRCVIAREMTKIHEEFWRGTLAEAKEFFSL 284
           +NE  TQIFYVPPHKL QF+EET   FGESR+CVIARE+TK+HEEFWRG++AEAK+ F +
Sbjct: 208 SNETRTQIFYVPPHKLSQFLEETTPYFGESRQCVIAREITKLHEEFWRGSIAEAKQEFLI 267

Query: 285 HQTKGEITLLIEGKLDSGVEAPSELQLEKELQELISSGHRLSMAVKLVASKTSASRKTVY 344
            Q KGEITLLIEGK ++  E P+E QLE+EL+ LIS GH LS AVK VA +TS  +K VY
Sbjct: 268 RQPKGEITLLIEGKEETKAENPTESQLEEELRGLISDGHSLSTAVKTVAERTSMRKKEVY 327

Query: 345 SIALRKFGNQLGADSD 361
           S+AL+KFG Q+  + +
Sbjct: 328 SLALKKFGKQIRVEEE 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144419.13.1e-19598.62uncharacterized protein LOC111014109 [Momordica charantia][more]
XP_023538391.16.4e-17284.85uncharacterized protein LOC111799188 [Cucurbita pepo subsp. pepo][more]
XP_022951884.11.1e-17185.12uncharacterized protein LOC111454620 [Cucurbita moschata][more]
XP_023002398.11.2e-17084.30uncharacterized protein LOC111496252 [Cucurbita maxima][more]
XP_038886193.12.1e-17084.02ribosomal RNA small subunit methyltransferase I [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P740384.7e-6145.56Ribosomal RNA small subunit methyltransferase I OS=Synechocystis sp. (strain PCC... [more]
Q9KGL23.0e-5543.40Ribosomal RNA small subunit methyltransferase I OS=Bacillus halodurans (strain A... [more]
P452985.8e-5141.91Ribosomal RNA small subunit methyltransferase I OS=Haemophilus influenzae (strai... [more]
Q87B702.9e-5042.80Ribosomal RNA small subunit methyltransferase I OS=Xylella fastidiosa (strain Te... [more]
Q9KUD96.4e-5042.70Ribosomal RNA small subunit methyltransferase I OS=Vibrio cholerae serotype O1 (... [more]
Match NameE-valueIdentityDescription
A0A6J1CS921.5e-19598.62uncharacterized protein LOC111014109 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A6J1GK225.3e-17285.12uncharacterized protein LOC111454620 OS=Cucurbita moschata OX=3662 GN=LOC1114546... [more]
A0A6J1KTF75.8e-17184.30uncharacterized protein LOC111496252 OS=Cucurbita maxima OX=3661 GN=LOC111496252... [more]
A0A1S3BBU61.7e-17084.66ribosomal RNA small subunit methyltransferase I OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LRJ71.7e-17085.21TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G36244... [more]
Match NameE-valueIdentityDescription
AT1G45110.13.8e-12271.20Tetrapyrrole (Corrin/Porphyrin) Methylases [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000878Tetrapyrrole methylasePFAMPF00590TP_methylasecoord: 79..278
e-value: 5.9E-30
score: 104.8
IPR014776Tetrapyrrole methylase, subdomain 2GENE3D3.30.950.10coord: 186..301
e-value: 7.8E-38
score: 131.0
IPR008189rRNA small subunit methyltransferase ITIGRFAMTIGR00096TIGR00096coord: 79..348
e-value: 2.3E-78
score: 261.3
IPR008189rRNA small subunit methyltransferase IPANTHERPTHR46111RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE Icoord: 63..358
IPR008189rRNA small subunit methyltransferase IHAMAPMF_0187716SrRNA_methyltr_Icoord: 78..297
score: 35.745682
IPR008189rRNA small subunit methyltransferase ICDDcd11648RsmIcoord: 80..296
e-value: 1.36796E-126
score: 360.545
IPR014777Tetrapyrrole methylase, subdomain 1GENE3D3.40.1010.10coord: 63..185
e-value: 1.2E-42
score: 146.6
NoneNo IPR availablePANTHERPTHR46111:SF1RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE Icoord: 63..358
IPR018063SAM-dependent methyltransferase RsmI, conserved sitePROSITEPS01296RSMIcoord: 156..167
IPR035996Tetrapyrrole methylase superfamilySUPERFAMILY53790Tetrapyrrole methylasecoord: 77..295

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS014521MS014521gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS014521.1-cdsMS014521.1-cds-scaffold553:579520..579735CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:580283..580350CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:580603..580699CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:580785..580913CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:581141..581249CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:581469..581613CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:581943..581985CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:582863..583036CDS
MS014521.1-cdsMS014521.1-cds-scaffold553:583516..583623CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS014521.1MS014521.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008168 methyltransferase activity