MS014259.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS014259.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Protein kinase superfamily protein
Location	scaffold5: 1714658 .. 1715712 (+)
Sequence length	501
RNA-Seq Expression	MS014259.1
Synteny	MS014259.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGGGCACAAGGAGTGGATCAACGAGGTGAACTTTCTGGGTGTGGTGAAGCATCCGAATCTTGTAAAATTAGTCGGATACTGTGCAGAGGATGATGAAAGGGGCATGCAAAGGCTTCTGGTTTATGAGCTCATGCGCAACAGAAGCCTGGAGGACCATCTGGTTCGAGTTTCTTCACCATTGTCATGGTTGGCAAGGCTTAAAATTGCTCAGGATGCAGCTCGAGGTCTTGCATATTTACATGAAGAAATGGATTTTCAGGTATAAATTAATGAGATTCTGGGTAGTTAAAATCTACTGTTGCCATCCTTGAATCTTGATGTTTCTCAGATGTTAATTATCTCGTCTTACCATGTGAAGTAAAACACAGAATTTCTGTCTTGTGGTCAGCATGTGTTTGATAGTTGCTTCTGCCTTTCGTATTTAAGTGTGTTGGATCTGCTTTTGTACGATGCAGCTAATTTTCCGCGACTTAAAAGCATCAAATATCCTGATAGATGAAGAATTTAATGCAAAGCTTTCAGATTTTGGACTGGCCAGGCAGGGTCCTCCCGAAGGAATCAGTCATGTGTCAACATCAGTAAGTTTTATCTTGTTGGAAATACTAAAAAATACAACTGAACTTGTAATGTAATGTACGTTGATAGTTGCTTTTTGTTAAGTAAGATCATTTTTCCAAATTAAAAACACTGTAAACTACTTCCTGATAAAGTACTTAATTACTGCTTTCTTTTTTAATATGTTTAACTTCAAAAGTTATCCTTCTAGTATGCAGGATCAACAAATCATTTTCTTGCCACACACTTGGATTTTAGCTTCTAAATAGGCATATGTTACATTTATTGCTTTTATGCTGTTACATCTGAAGCGATGTTCTGTTGTAATTCTCAAATACAGAAGATGTGTCCTTCTGATGACAATTTATTTGTGTGTTCAAGGTTGTGGGTACAATAGGCTATGCAGCTCCAGAGTATGTTCAGACTGGAAGGCTGACTGCAAAGAGCGATGTGTGGAGCTTCGGGGTGGTTCTCTATGAACTCATTACAGGTCGACGA

mRNA sequence

CAGGGGCACAAGGAGTGGATCAACGAGGTGAACTTTCTGGGTGTGGTGAAGCATCCGAATCTTGTAAAATTAGTCGGATACTGTGCAGAGGATGATGAAAGGGGCATGCAAAGGCTTCTGGTTTATGAGCTCATGCGCAACAGAAGCCTGGAGGACCATCTGGTTCGAGTTTCTTCACCATTGTCATGGTTGGCAAGGCTTAAAATTGCTCAGGATGCAGCTCGAGGTCTTGCATATTTACATGAAGAAATGGATTTTCAGCTAATTTTCCGCGACTTAAAAGCATCAAATATCCTGATAGATGAAGAATTTAATGCAAAGCTTTCAGATTTTGGACTGGCCAGGCAGGGTCCTCCCGAAGGAATCAGTCATGTGTCAACATCAGTTGTGGGTACAATAGGCTATGCAGCTCCAGAGTATGTTCAGACTGGAAGGCTGACTGCAAAGAGCGATGTGTGGAGCTTCGGGGTGGTTCTCTATGAACTCATTACAGGTCGACGA

Coding sequence (CDS)

CAGGGGCACAAGGAGTGGATCAACGAGGTGAACTTTCTGGGTGTGGTGAAGCATCCGAATCTTGTAAAATTAGTCGGATACTGTGCAGAGGATGATGAAAGGGGCATGCAAAGGCTTCTGGTTTATGAGCTCATGCGCAACAGAAGCCTGGAGGACCATCTGGTTCGAGTTTCTTCACCATTGTCATGGTTGGCAAGGCTTAAAATTGCTCAGGATGCAGCTCGAGGTCTTGCATATTTACATGAAGAAATGGATTTTCAGCTAATTTTCCGCGACTTAAAAGCATCAAATATCCTGATAGATGAAGAATTTAATGCAAAGCTTTCAGATTTTGGACTGGCCAGGCAGGGTCCTCCCGAAGGAATCAGTCATGTGTCAACATCAGTTGTGGGTACAATAGGCTATGCAGCTCCAGAGTATGTTCAGACTGGAAGGCTGACTGCAAAGAGCGATGTGTGGAGCTTCGGGGTGGTTCTCTATGAACTCATTACAGGTCGACGA

Protein sequence

QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSPLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPEGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR

Homology

[Close]

BLAST of MS014259.1 vs. NCBI nr
Match: XP_022148407.1 (probable serine/threonine-protein kinase PBL19 isoform X2 [Momordica charantia])

HSP 1 Score: 339.7 bits (870), Expect = 1.4e-89
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP
Sbjct: 178 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 237

Query: 61  LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 120
           LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE
Sbjct: 238 LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 297

Query: 121 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 298 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 344

[Close]

BLAST of MS014259.1 vs. NCBI nr
Match: XP_022148406.1 (probable serine/threonine-protein kinase PBL19 isoform X1 [Momordica charantia])

HSP 1 Score: 339.7 bits (870), Expect = 1.4e-89
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP
Sbjct: 182 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 241

Query: 61  LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 120
           LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE
Sbjct: 242 LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 301

Query: 121 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 302 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 348

[Close]

BLAST of MS014259.1 vs. NCBI nr
Match: XP_008464123.1 (PREDICTED: probable receptor-like protein kinase At5g47070 [Cucumis melo])

HSP 1 Score: 332.4 bits (851), Expect = 2.2e-87
Identity = 165/168 (98.21%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH-LVRVSS 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH LVRVS 
Sbjct: 130 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLLVRVSP 189

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
           PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNIL+DEEFNAKLSDFGLARQGPP
Sbjct: 190 PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILLDEEFNAKLSDFGLARQGPP 249

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 250 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 297

[Close]

BLAST of MS014259.1 vs. NCBI nr
Match: XP_038902228.1 (serine/threonine-protein kinase PCRK1 [Benincasa hispida])

HSP 1 Score: 332.4 bits (851), Expect = 2.2e-87
Identity = 165/168 (98.21%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH-LVRVSS 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH LVRVS 
Sbjct: 136 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLLVRVSP 195

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
           PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNIL+DEEFNAKLSDFGLARQGPP
Sbjct: 196 PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILLDEEFNAKLSDFGLARQGPP 255

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 256 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 303

[Close]

BLAST of MS014259.1 vs. NCBI nr
Match: KAE8646967.1 (hypothetical protein Csa_020710 [Cucumis sativus])

HSP 1 Score: 332.4 bits (851), Expect = 2.2e-87
Identity = 165/168 (98.21%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH-LVRVSS 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH LVRVS 
Sbjct: 6   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLLVRVSP 65

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
           PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNIL+DEEFNAKLSDFGLARQGPP
Sbjct: 66  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILLDEEFNAKLSDFGLARQGPP 125

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 126 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 173

[Close]

BLAST of MS014259.1 vs. ExPASy Swiss-Prot
Match: Q9SF86 (Serine/threonine-protein kinase PCRK1 OS=Arabidopsis thaliana OX=3702 GN=PCRK1 PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.2e-70
Identity = 127/168 (75.60%), Postives = 148/168 (88.10%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHL-VRVSS 60
           QGHKEW+ EVNFLG+V+H NLVKL+GYCAEDDERG+QRLLVYE M NRS+E HL  R  +
Sbjct: 126 QGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHLSPRSLT 185

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
            L+W  RL+IAQDAARGL YLHEEM+FQ+IFRD K+SNIL+DE++ AKLSDFGLAR GP 
Sbjct: 186 VLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGLARLGPS 245

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EG++HVST VVGT+GYAAPEY+QTGRLT+KSDVW +GV LYELITGRR
Sbjct: 246 EGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRR 293

[Close]

BLAST of MS014259.1 vs. ExPASy Swiss-Prot
Match: Q9LZF8 (Serine/threonine-protein kinase PCRK2 OS=Arabidopsis thaliana OX=3702 GN=PCRK2 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 4.9e-69
Identity = 126/168 (75.00%), Postives = 145/168 (86.31%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHL-VRVSS 60
           QGHKEW+ EVNFLGVV+H NLVKL+G+CAEDDERG+QRLLVYE M N+S+E HL  R  +
Sbjct: 123 QGHKEWVTEVNFLGVVEHSNLVKLLGHCAEDDERGIQRLLVYEYMPNQSVEFHLSPRSPT 182

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
            L+W  RL+IAQDAARGL YLHEEMDFQ+IFRD K+SNIL+DE + AKLSDFGLAR GP 
Sbjct: 183 VLTWDLRLRIAQDAARGLTYLHEEMDFQIIFRDFKSSNILLDENWTAKLSDFGLARLGPS 242

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
            G SHVST VVGT+GYAAPEY+QTGRLT+KSDVW +GV +YELITGRR
Sbjct: 243 PGSSHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFIYELITGRR 290

[Close]

BLAST of MS014259.1 vs. ExPASy Swiss-Prot
Match: Q9LFP7 (Serine/threonine-protein kinase PBL34 OS=Arabidopsis thaliana OX=3702 GN=PBL34 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 5.0e-58
Identity = 111/167 (66.47%), Postives = 131/167 (78.44%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 60
           QGHKEW+ E+NFLG + HPNLVKLVGYC EDD    QRLLVYE M   SLE+HL R S P
Sbjct: 188 QGHKEWLAEINFLGNLLHPNLVKLVGYCIEDD----QRLLVYEFMPRGSLENHLFRRSLP 247

Query: 61  LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 120
           L W  R+KIA  AA+GL++LHEE    +I+RD K SNIL+D ++NAKLSDFGLA+  P E
Sbjct: 248 LPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILLDADYNAKLSDFGLAKDAPDE 307

Query: 121 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           G +HVST V+GT GYAAPEYV TG LT+KSDV+SFGVVL E++TGRR
Sbjct: 308 GKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMLTGRR 350

[Close]

BLAST of MS014259.1 vs. ExPASy Swiss-Prot
Match: Q9SRH7 (Serine/threonine-protein kinase PBL35 OS=Arabidopsis thaliana OX=3702 GN=PBL35 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 6.6e-58
Identity = 111/167 (66.47%), Postives = 131/167 (78.44%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 60
           QGHKEW+ E+N+LG + HPNLVKLVGYC EDD    QRLLVYE M   SLE+HL R S P
Sbjct: 182 QGHKEWLAEINYLGNLLHPNLVKLVGYCIEDD----QRLLVYEFMPRGSLENHLFRRSLP 241

Query: 61  LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 120
           L W  R+KIA  AA+GL++LHEE    +I+RD K SNIL+D E+NAKLSDFGLA+  P E
Sbjct: 242 LPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILLDGEYNAKLSDFGLAKDAPDE 301

Query: 121 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           G +HVST V+GT GYAAPEYV TG LT+KSDV+SFGVVL E++TGRR
Sbjct: 302 GKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMLTGRR 344

[Close]

BLAST of MS014259.1 vs. ExPASy Swiss-Prot
Match: F4J0D2 (Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 PE=1 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 2.5e-57
Identity = 110/167 (65.87%), Postives = 130/167 (77.84%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 60
           QGHKEW+ E+NFLG + HP+LVKLVGYC E+D    QRLLVYE M   SLE+HL R + P
Sbjct: 172 QGHKEWLAEINFLGNLVHPSLVKLVGYCMEED----QRLLVYEFMPRGSLENHLFRRTLP 231

Query: 61  LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 120
           L W  R+KIA  AA+GLA+LHEE +  +I+RD K SNIL+D E+NAKLSDFGLA+  P E
Sbjct: 232 LPWSVRMKIALGAAKGLAFLHEEAEKPVIYRDFKTSNILLDGEYNAKLSDFGLAKDAPDE 291

Query: 121 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
             SHVST V+GT GYAAPEYV TG LT KSDV+SFGVVL E++TGRR
Sbjct: 292 KKSHVSTRVMGTYGYAAPEYVMTGHLTTKSDVYSFGVVLLEILTGRR 334

[Close]

BLAST of MS014259.1 vs. ExPASy TrEMBL
Match: A0A6J1D5A3 (probable serine/threonine-protein kinase PBL19 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017062 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 6.8e-90
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP
Sbjct: 182 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 241

Query: 61  LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 120
           LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE
Sbjct: 242 LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 301

Query: 121 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 302 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 348

[Close]

BLAST of MS014259.1 vs. ExPASy TrEMBL
Match: A0A6J1D4Z6 (probable serine/threonine-protein kinase PBL19 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017062 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 6.8e-90
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP
Sbjct: 178 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVRVSSP 237

Query: 61  LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 120
           LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE
Sbjct: 238 LSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPPE 297

Query: 121 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 298 GISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 344

[Close]

BLAST of MS014259.1 vs. ExPASy TrEMBL
Match: A0A0A0KBR9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G190360 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.1e-87
Identity = 165/168 (98.21%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH-LVRVSS 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH LVRVS 
Sbjct: 130 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLLVRVSP 189

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
           PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNIL+DEEFNAKLSDFGLARQGPP
Sbjct: 190 PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILLDEEFNAKLSDFGLARQGPP 249

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 250 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 297

[Close]

BLAST of MS014259.1 vs. ExPASy TrEMBL
Match: A0A1S3CKR9 (probable receptor-like protein kinase At5g47070 OS=Cucumis melo OX=3656 GN=LOC103502080 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.1e-87
Identity = 165/168 (98.21%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH-LVRVSS 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH LVRVS 
Sbjct: 130 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLLVRVSP 189

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
           PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNIL+DEEFNAKLSDFGLARQGPP
Sbjct: 190 PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILLDEEFNAKLSDFGLARQGPP 249

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 250 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 297

[Close]

BLAST of MS014259.1 vs. ExPASy TrEMBL
Match: A0A6J1L4R0 (probable serine/threonine-protein kinase PBL19 OS=Cucurbita maxima OX=3661 GN=LOC111499878 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.8e-87
Identity = 164/168 (97.62%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDH-LVRVSS 60
           QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMR RSLEDH LVRVSS
Sbjct: 136 QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRKRSLEDHLLVRVSS 195

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
           PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNIL+DEEFNAKLSDFGLARQGPP
Sbjct: 196 PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILLDEEFNAKLSDFGLARQGPP 255

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EGISHVSTSVVGT+GYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR
Sbjct: 256 EGISHVSTSVVGTVGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 303

[Close]

BLAST of MS014259.1 vs. TAIR 10
Match: AT2G28940.1 (Protein kinase superfamily protein )

HSP 1 Score: 301.6 bits (771), Expect = 3.9e-82
Identity = 147/169 (86.98%), Postives = 156/169 (92.31%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVR--VS 60
           QGHKEWINEVNFLGVV HPNLVKLVGYCA+DDERGMQRLLVYELM N+SLEDHLV   VS
Sbjct: 27  QGHKEWINEVNFLGVVNHPNLVKLVGYCADDDERGMQRLLVYELMCNKSLEDHLVGRVVS 86

Query: 61  SPLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGP 120
             L W+ RLKIAQDAA+GLAYLHEEMDFQLIFRD K+SNIL+DE F AKLSDFGLARQGP
Sbjct: 87  VSLPWMMRLKIAQDAAQGLAYLHEEMDFQLIFRDFKSSNILLDERFGAKLSDFGLARQGP 146

Query: 121 PEGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           PEG+ HVSTSVVGT+GYAAPEYVQTG+LTAKSDVWSFGVVLYELITGRR
Sbjct: 147 PEGLGHVSTSVVGTVGYAAPEYVQTGKLTAKSDVWSFGVVLYELITGRR 195

[Close]

BLAST of MS014259.1 vs. TAIR 10
Match: AT2G28940.2 (Protein kinase superfamily protein )

HSP 1 Score: 301.6 bits (771), Expect = 3.9e-82
Identity = 147/169 (86.98%), Postives = 156/169 (92.31%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVR--VS 60
           QGHKEWINEVNFLGVV HPNLVKLVGYCA+DDERGMQRLLVYELM N+SLEDHLV   VS
Sbjct: 146 QGHKEWINEVNFLGVVNHPNLVKLVGYCADDDERGMQRLLVYELMCNKSLEDHLVGRVVS 205

Query: 61  SPLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGP 120
             L W+ RLKIAQDAA+GLAYLHEEMDFQLIFRD K+SNIL+DE F AKLSDFGLARQGP
Sbjct: 206 VSLPWMMRLKIAQDAAQGLAYLHEEMDFQLIFRDFKSSNILLDERFGAKLSDFGLARQGP 265

Query: 121 PEGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           PEG+ HVSTSVVGT+GYAAPEYVQTG+LTAKSDVWSFGVVLYELITGRR
Sbjct: 266 PEGLGHVSTSVVGTVGYAAPEYVQTGKLTAKSDVWSFGVVLYELITGRR 314

[Close]

BLAST of MS014259.1 vs. TAIR 10
Match: AT2G39110.1 (Protein kinase superfamily protein )

HSP 1 Score: 275.4 bits (703), Expect = 3.0e-74
Identity = 128/169 (75.74%), Postives = 150/169 (88.76%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHLVR--VS 60
           QGHKEW+ EVN LGVV+HPNLVKL+GYCAEDDERG+QRLLVYE ++NRS++DHL    + 
Sbjct: 132 QGHKEWVTEVNVLGVVEHPNLVKLIGYCAEDDERGIQRLLVYEYVQNRSVQDHLSNRFIV 191

Query: 61  SPLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGP 120
           +PL W  RLKIAQD ARGLAYLH+ M+FQ+IFRD K+SNIL+DE +NAKLSDFGLAR GP
Sbjct: 192 TPLPWSTRLKIAQDTARGLAYLHQGMEFQIIFRDFKSSNILLDENWNAKLSDFGLARMGP 251

Query: 121 PEGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
            +GI+HVST+VVGTIGYAAPEY+QTG LTAKSDVWS+G+ LYELITGRR
Sbjct: 252 SDGITHVSTAVVGTIGYAAPEYIQTGHLTAKSDVWSYGIFLYELITGRR 300

[Close]

BLAST of MS014259.1 vs. TAIR 10
Match: AT3G09830.1 (Protein kinase superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 8.2e-72
Identity = 127/168 (75.60%), Postives = 148/168 (88.10%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHL-VRVSS 60
           QGHKEW+ EVNFLG+V+H NLVKL+GYCAEDDERG+QRLLVYE M NRS+E HL  R  +
Sbjct: 126 QGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHLSPRSLT 185

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
            L+W  RL+IAQDAARGL YLHEEM+FQ+IFRD K+SNIL+DE++ AKLSDFGLAR GP 
Sbjct: 186 VLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGLARLGPS 245

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EG++HVST VVGT+GYAAPEY+QTGRLT+KSDVW +GV LYELITGRR
Sbjct: 246 EGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRR 293

[Close]

BLAST of MS014259.1 vs. TAIR 10
Match: AT3G09830.2 (Protein kinase superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 8.2e-72
Identity = 127/168 (75.60%), Postives = 148/168 (88.10%), Query Frame = 0


Query: 1   QGHKEWINEVNFLGVVKHPNLVKLVGYCAEDDERGMQRLLVYELMRNRSLEDHL-VRVSS 60
           QGHKEW+ EVNFLG+V+H NLVKL+GYCAEDDERG+QRLLVYE M NRS+E HL  R  +
Sbjct: 126 QGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHLSPRSLT 185

Query: 61  PLSWLARLKIAQDAARGLAYLHEEMDFQLIFRDLKASNILIDEEFNAKLSDFGLARQGPP 120
            L+W  RL+IAQDAARGL YLHEEM+FQ+IFRD K+SNIL+DE++ AKLSDFGLAR GP 
Sbjct: 186 VLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGLARLGPS 245

Query: 121 EGISHVSTSVVGTIGYAAPEYVQTGRLTAKSDVWSFGVVLYELITGRR 168
           EG++HVST VVGT+GYAAPEY+QTGRLT+KSDVW +GV LYELITGRR
Sbjct: 246 EGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRR 293

The following BLAST results are available for this feature:

BLAST of MS014259.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148407.1	1.4e-89	100.00	probable serine/threonine-protein kinase PBL19 isoform X2 [Momordica charantia]	[more]
XP_022148406.1	1.4e-89	100.00	probable serine/threonine-protein kinase PBL19 isoform X1 [Momordica charantia]	[more]
XP_008464123.1	2.2e-87	98.21	PREDICTED: probable receptor-like protein kinase At5g47070 [Cucumis melo]	[more]
XP_038902228.1	2.2e-87	98.21	serine/threonine-protein kinase PCRK1 [Benincasa hispida]	[more]
KAE8646967.1	2.2e-87	98.21	hypothetical protein Csa_020710 [Cucumis sativus]	[more]

BLAST of MS014259.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SF86	1.2e-70	75.60	Serine/threonine-protein kinase PCRK1 OS=Arabidopsis thaliana OX=3702 GN=PCRK1 P...	[more]
Q9LZF8	4.9e-69	75.00	Serine/threonine-protein kinase PCRK2 OS=Arabidopsis thaliana OX=3702 GN=PCRK2 P...	[more]
Q9LFP7	5.0e-58	66.47	Serine/threonine-protein kinase PBL34 OS=Arabidopsis thaliana OX=3702 GN=PBL34 P...	[more]
Q9SRH7	6.6e-58	66.47	Serine/threonine-protein kinase PBL35 OS=Arabidopsis thaliana OX=3702 GN=PBL35 P...	[more]
F4J0D2	2.5e-57	65.87	Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 P...	[more]

BLAST of MS014259.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5A3	6.8e-90	100.00	probable serine/threonine-protein kinase PBL19 isoform X1 OS=Momordica charantia...	[more]
A0A6J1D4Z6	6.8e-90	100.00	probable serine/threonine-protein kinase PBL19 isoform X2 OS=Momordica charantia...	[more]
A0A0A0KBR9	1.1e-87	98.21	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G190...	[more]
A0A1S3CKR9	1.1e-87	98.21	probable receptor-like protein kinase At5g47070 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1L4R0	1.8e-87	97.62	probable serine/threonine-protein kinase PBL19 OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of MS014259.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28940.1	3.9e-82	86.98	Protein kinase superfamily protein	[more]
AT2G28940.2	3.9e-82	86.98	Protein kinase superfamily protein	[more]
AT2G39110.1	3.0e-74	75.74	Protein kinase superfamily protein	[more]
AT3G09830.1	8.2e-72	75.60	Protein kinase superfamily protein	[more]
AT3G09830.2	8.2e-72	75.60	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 3..167 e-value: 5.0E-9 score: -5.9
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 4..166 e-value: 1.4E-37 score: 129.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..167 score: 32.668636
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 46..167 e-value: 1.2E-47 score: 163.9
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 1..45 e-value: 5.5E-12 score: 47.7
None	No IPR available	PIRSR	PIRSR000606-51	PIRSR000606-51	coord: 11..166 e-value: 2.5E-20 score: 70.2
None	No IPR available	PANTHER	PTHR45621:SF53	SERINE/THREONINE-PROTEIN KINASE PCRK1	coord: 1..167
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 1..167
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..167

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS014259	MS014259	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS014259.1-cds	MS014259.1-cds-scaffold5:1714658..1714918	CDS
MS014259.1-cds	MS014259.1-cds-scaffold5:1715115..1715237	CDS
MS014259.1-cds	MS014259.1-cds-scaffold5:1715596..1715712	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS014259.1	MS014259.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity