MS014085.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS014085.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA-directed RNA polymerase III subunit rpc6
Location	scaffold5: 476719 .. 477432 (+)
Sequence length	714
RNA-Seq Expression	MS014085.1
Synteny	MS014085.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAGATCACAAGGACCTTCATCACTTAAAAGAAAAAGGCCAGACTCAAGCTCTGGAGGAGCTCTGATCGAGCATGAAAAAGTGCTCTATGATCTGATTTGCAGCAAACAAAGCATGGGGATTTGGATTGGAGACATTAAAAGAGAAACAAAGTATGCTGACAGTATAGTAACTAAATCACTCAAAGCCCTCCAAGCCAAAGGCATGATAAAACTGGTTGAACATTATCAGAACAAAGGGAAGAAATTCTACCTGTCGAAAGATTTCGAGCCATCGAAAGAACTAACGGGCGGTAATTGGTATAGCGAGGGGAAGCTCGACAGGGAATACATCAATACTCTTAAAGGATTCTGTGCAAGAATGATACAAAAGATGAAGGTGGCTACAATTGAAGGGATTGTGGACTCTTTCAAAAAGAGTGGAGCGTCCAAGGTGGACTTGTCTAGAGAGCAAATTCAGGAGATTGTAAATGCTCTGGTTTTGGACAATGAAGTCATGGAGGTGAAGAGCACTGGATCAGGGGAATTTGATTTTATCCCTATTGGGAAGATCTGTTATAAGTGCTATGCCAAAGGAGGTGTCAAAGGGGAGCCAAAAACGGGGGCCATGGCTTCCATTCCTTGTGGAGTCTGTCCTCGGATTAGTCATTGTACGCCAAACGGCGTCATTTCGCCTAGAACATGCGTGTACTACACCAAGTGGTTAGATTTT

mRNA sequence

ATGAGCAGATCACAAGGACCTTCATCACTTAAAAGAAAAAGGCCAGACTCAAGCTCTGGAGGAGCTCTGATCGAGCATGAAAAAGTGCTCTATGATCTGATTTGCAGCAAACAAAGCATGGGGATTTGGATTGGAGACATTAAAAGAGAAACAAAGTATGCTGACAGTATAGTAACTAAATCACTCAAAGCCCTCCAAGCCAAAGGCATGATAAAACTGGTTGAACATTATCAGAACAAAGGGAAGAAATTCTACCTGTCGAAAGATTTCGAGCCATCGAAAGAACTAACGGGCGGTAATTGGTATAGCGAGGGGAAGCTCGACAGGGAATACATCAATACTCTTAAAGGATTCTGTGCAAGAATGATACAAAAGATGAAGGTGGCTACAATTGAAGGGATTGTGGACTCTTTCAAAAAGAGTGGAGCGTCCAAGGTGGACTTGTCTAGAGAGCAAATTCAGGAGATTGTAAATGCTCTGGTTTTGGACAATGAAGTCATGGAGGTGAAGAGCACTGGATCAGGGGAATTTGATTTTATCCCTATTGGGAAGATCTGTTATAAGTGCTATGCCAAAGGAGGTGTCAAAGGGGAGCCAAAAACGGGGGCCATGGCTTCCATTCCTTGTGGAGTCTGTCCTCGGATTAGTCATTGTACGCCAAACGGCGTCATTTCGCCTAGAACATGCGTGTACTACACCAAGTGGTTAGATTTT

Coding sequence (CDS)

ATGAGCAGATCACAAGGACCTTCATCACTTAAAAGAAAAAGGCCAGACTCAAGCTCTGGAGGAGCTCTGATCGAGCATGAAAAAGTGCTCTATGATCTGATTTGCAGCAAACAAAGCATGGGGATTTGGATTGGAGACATTAAAAGAGAAACAAAGTATGCTGACAGTATAGTAACTAAATCACTCAAAGCCCTCCAAGCCAAAGGCATGATAAAACTGGTTGAACATTATCAGAACAAAGGGAAGAAATTCTACCTGTCGAAAGATTTCGAGCCATCGAAAGAACTAACGGGCGGTAATTGGTATAGCGAGGGGAAGCTCGACAGGGAATACATCAATACTCTTAAAGGATTCTGTGCAAGAATGATACAAAAGATGAAGGTGGCTACAATTGAAGGGATTGTGGACTCTTTCAAAAAGAGTGGAGCGTCCAAGGTGGACTTGTCTAGAGAGCAAATTCAGGAGATTGTAAATGCTCTGGTTTTGGACAATGAAGTCATGGAGGTGAAGAGCACTGGATCAGGGGAATTTGATTTTATCCCTATTGGGAAGATCTGTTATAAGTGCTATGCCAAAGGAGGTGTCAAAGGGGAGCCAAAAACGGGGGCCATGGCTTCCATTCCTTGTGGAGTCTGTCCTCGGATTAGTCATTGTACGCCAAACGGCGTCATTTCGCCTAGAACATGCGTGTACTACACCAAGTGGTTAGATTTT

Protein sequence

MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF

Homology

[Close]

BLAST of MS014085.1 vs. NCBI nr
Match: XP_022149571.1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >XP_022149572.1 DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia])

HSP 1 Score: 484.2 bits (1245), Expect = 6.5e-133
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK 60
           MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK
Sbjct: 1   MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK 60

Query: 61  SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCA 120
           SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFC 
Sbjct: 61  SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCG 120

Query: 121 RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI 180
           RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI
Sbjct: 121 RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI 180

Query: 181 PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF
Sbjct: 181 PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

[Close]

BLAST of MS014085.1 vs. NCBI nr
Match: XP_022970903.1 (DNA-directed RNA polymerase III subunit rpc6 [Cucurbita maxima])

HSP 1 Score: 444.9 bits (1143), Expect = 4.4e-121
Identity = 216/240 (90.00%), Postives = 232/240 (96.67%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60
           MSRSQGPSSLKRKRP+ +   GGALIEHEKVLYDLICSKQSMGIWIGD+KRETKY D++V
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120
           TKSLKALQA+G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLDREYI+TLKGF
Sbjct: 61  TKSLKALQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQKMKVATIEGIVD+FKKSGASKVDLSREQI+EIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDAFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           FIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCTPNG+ISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

[Close]

BLAST of MS014085.1 vs. NCBI nr
Match: KAG6604735.1 (DNA-directed RNA polymerase III subunit rpc6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 444.5 bits (1142), Expect = 5.7e-121
Identity = 216/240 (90.00%), Postives = 231/240 (96.25%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60
           MSRSQGPSSLKRKRP+ +   GGALIEHEKVLYDLICSKQSMGIWIGD+KRETKY D++V
Sbjct: 89  MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 148

Query: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120
           TKSLKALQ +G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLDREYI+TLKGF
Sbjct: 149 TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 208

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQI+EIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 209 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 268

Query: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           FIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCTPNG+ISPRTCVYYTKWL+F
Sbjct: 269 FIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 327

[Close]

BLAST of MS014085.1 vs. NCBI nr
Match: XP_022946967.1 (DNA-directed RNA polymerase III subunit rpc6 [Cucurbita moschata] >XP_023532566.1 DNA-directed RNA polymerase III subunit rpc6 [Cucurbita pepo subsp. pepo] >KAG7034865.1 DNA-directed RNA polymerase III subunit rpc6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 444.5 bits (1142), Expect = 5.7e-121
Identity = 216/240 (90.00%), Postives = 231/240 (96.25%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60
           MSRSQGPSSLKRKRP+ +   GGALIEHEKVLYDLICSKQSMGIWIGD+KRETKY D++V
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120
           TKSLKALQ +G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLDREYI+TLKGF
Sbjct: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQI+EIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           FIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCTPNG+ISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

[Close]

BLAST of MS014085.1 vs. NCBI nr
Match: XP_022149573.1 (DNA-directed RNA polymerase III subunit rpc6 isoform X2 [Momordica charantia] >XP_022149574.1 DNA-directed RNA polymerase III subunit rpc6 isoform X2 [Momordica charantia])

HSP 1 Score: 409.5 bits (1051), Expect = 2.0e-110
Identity = 198/199 (99.50%), Postives = 198/199 (99.50%), Query Frame = 0


Query: 40  MGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGG 99
           MGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGG
Sbjct: 1   MGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGG 60

Query: 100 NWYSEGKLDREYINTLKGFCARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNA 159
           NWYSEGKLDREYINTLKGFC RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNA
Sbjct: 61  NWYSEGKLDREYINTLKGFCGRMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNA 120

Query: 160 LVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCT 219
           LVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCT
Sbjct: 121 LVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCT 180

Query: 220 PNGVISPRTCVYYTKWLDF 239
           PNGVISPRTCVYYTKWLDF
Sbjct: 181 PNGVISPRTCVYYTKWLDF 199

[Close]

BLAST of MS014085.1 vs. ExPASy Swiss-Prot
Match: Q86JM3 (DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=44689 GN=polr3f PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-24
Identity = 70/243 (28.81%), Postives = 119/243 (48.97%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRP-DSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVT 60
           +  S   +S K   P D +    L   + ++Y LI +  + G W  ++K  + Y    +T
Sbjct: 52  IQNSDETTSYKEVNPEDQAKFRGLGVEDFLIYQLIEASSNTGAWTRELKNSSGYQQVQIT 111

Query: 61  KSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFC 120
           K LK L+++ +IK V+  Q+  KK Y+  + EPS+E+TGG  YS+   D +YI  +K   
Sbjct: 112 KILKTLESRKLIKSVKSIQSGRKKVYMLYNMEPSREITGGQLYSDQSYDHQYIQIMKMHI 171

Query: 121 ARMIQKMKVATIEGIVDSFKK--SGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEF 180
              +       +  I+   +K    A+   L  E IQ +VN ++ D ++ E++ T  G  
Sbjct: 172 KTFVDNKGAVDLADIITYLRKVAEEATSQSLGPEDIQALVNTVIYDGDIEEMRDTRMGAM 231

Query: 181 DFIPIGK---ICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTK 238
               +G+   I YK       K         ++PCG CP    C+ NG++SP+ CVY+ +
Sbjct: 232 ----LGRRSGILYK-----PTKTPIPVNNFCNMPCGNCPVFDICSDNGLVSPKRCVYFKQ 285

[Close]

BLAST of MS014085.1 vs. ExPASy Swiss-Prot
Match: Q2T9S3 (DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.5e-23
Identity = 73/244 (29.92%), Postives = 115/244 (47.13%), Query Frame = 0


Query: 16  DSSSGGAL---IEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTKSLKALQAKGMIK 75
           DS + G +      EK++Y +I    + GIW  DI+ ++    + + K LK L++K +IK
Sbjct: 78  DSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIK 137

Query: 76  LVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCARMIQKMKVATIE 135
            V+      KK Y+  + +P + +TGG WYS+   + E++  L   C + +Q       E
Sbjct: 138 AVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQNKAETARE 197

Query: 136 GIVD-------SFKKS----------GASKVDLSREQIQEIVNALVLDNEV-MEVKSTGS 195
              +       SF  S          G SKV+LS E I+ I+N L+ D +V M + +   
Sbjct: 198 SKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKE 257

Query: 196 GEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTK 239
           G    +      Y+  +       P TG +   PCG+CP    C   G ISP  C+Y T+
Sbjct: 258 GTVGSVDGHMKLYRAVS----PIIPPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTE 316

[Close]

BLAST of MS014085.1 vs. ExPASy Swiss-Prot
Match: Q9H1D9 (DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.4e-23
Identity = 73/244 (29.92%), Postives = 114/244 (46.72%), Query Frame = 0


Query: 16  DSSSGGAL---IEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTKSLKALQAKGMIK 75
           DS + G +      EK++Y +I    + GIW  DI+ ++    + + K LK L++K +IK
Sbjct: 78  DSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIK 137

Query: 76  LVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCARMIQKMKVATIE 135
            V+      KK Y+  + +P + +TGG WYS+   + E++  L   C + +Q       E
Sbjct: 138 AVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARE 197

Query: 136 GIVD-------SFKKS----------GASKVDLSREQIQEIVNALVLDNEV-MEVKSTGS 195
              +       SF  S          G SKV+LS E I+ I+N L+ D +V M + +   
Sbjct: 198 SKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKE 257

Query: 196 GEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTK 239
           G    +      Y+          P TG +   PCG+CP    C   G ISP  C+Y T+
Sbjct: 258 GTVGSVDGHMKLYRAVN----PIIPPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTE 316

[Close]

BLAST of MS014085.1 vs. ExPASy Swiss-Prot
Match: Q921X6 (DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.4e-23
Identity = 74/244 (30.33%), Postives = 117/244 (47.95%), Query Frame = 0


Query: 16  DSSSGGAL---IEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTKSLKALQAKGMIK 75
           DS + G +      EK++Y +I    + GIW  DI+ ++    + + K LK L++K +IK
Sbjct: 78  DSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIK 137

Query: 76  LVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCARMIQKMKVATIE 135
            V+      KK Y+  + +P + +TGG WYS+   + E++  L   C + +Q       E
Sbjct: 138 AVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARE 197

Query: 136 GIVD-------SFKKS----------GASKVDLSREQIQEIVNALVLDNEV-MEVKSTGS 195
              +       SF  S          G SKV+LS E I+ I+N L+ D +V M + +   
Sbjct: 198 SKQNPVIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKE 257

Query: 196 GEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTK 239
           G    +      Y+  A   +   P TG + + PCG+CP    C   G ISP  C+Y T+
Sbjct: 258 GTVGSVDGHMKLYR--AVNPIL--PPTGVVRA-PCGLCPVFEDCHEGGEISPSNCIYMTE 316

[Close]

BLAST of MS014085.1 vs. ExPASy Swiss-Prot
Match: P91529 (Probable DNA-directed RNA polymerase III subunit RPC6 OS=Caenorhabditis elegans OX=6239 GN=W09C3.4 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.9e-19
Identity = 68/240 (28.33%), Postives = 112/240 (46.67%), Query Frame = 0


Query: 5   QGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTKSLKA 64
           Q P  + R + ++S   A  E E+V+Y LI   ++ GIWI +++  +      + K+LK+
Sbjct: 63  QAPGGVLRLKVNTSEQIAGTEEEQVIYSLIEESKTRGIWIKELREGSGLNQLQLRKTLKS 122

Query: 65  LQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCARMIQ 124
           L+ K +IK ++      +K Y+    EP   LTGG +YS+ +LD E INTL   C     
Sbjct: 123 LETKKLIKTIKAV-GTTRKCYILFSIEPDMSLTGGTFYSDQQLDSELINTLISVCGSYAA 182

Query: 125 KMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLD--------NEVMEVKSTGSGE 184
             +   I+   ++ +    +     +E  Q I    VL+          ++EV +   G 
Sbjct: 183 SRRKHAIDENPNNIQMQREASYIRPQEIAQFITEKRVLNVPLSLEDLERILEV-AVLDGT 242

Query: 185 FDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWL 237
            +    GKI   C  +  +        + S+PC +CP +  C P  VISP++C Y   WL
Sbjct: 243 IERRADGKI-RACPPRSSI------SPLVSVPCAICPVVEDCRPGYVISPQSCDYMKNWL 293

[Close]

BLAST of MS014085.1 vs. ExPASy TrEMBL
Match: A0A6J1D8D1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017972 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 3.2e-133
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK 60
           MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK
Sbjct: 1   MSRSQGPSSLKRKRPDSSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTK 60

Query: 61  SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCA 120
           SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFC 
Sbjct: 61  SLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCG 120

Query: 121 RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI 180
           RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI
Sbjct: 121 RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFI 180

Query: 181 PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF
Sbjct: 181 PIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

[Close]

BLAST of MS014085.1 vs. ExPASy TrEMBL
Match: A0A6J1I599 (DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita maxima OX=3661 GN=LOC111469737 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.1e-121
Identity = 216/240 (90.00%), Postives = 232/240 (96.67%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60
           MSRSQGPSSLKRKRP+ +   GGALIEHEKVLYDLICSKQSMGIWIGD+KRETKY D++V
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120
           TKSLKALQA+G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLDREYI+TLKGF
Sbjct: 61  TKSLKALQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQKMKVATIEGIVD+FKKSGASKVDLSREQI+EIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDAFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           FIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCTPNG+ISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

[Close]

BLAST of MS014085.1 vs. ExPASy TrEMBL
Match: A0A6J1G553 (DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita moschata OX=3662 GN=LOC111450988 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.8e-121
Identity = 216/240 (90.00%), Postives = 231/240 (96.25%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60
           MSRSQGPSSLKRKRP+ +   GGALIEHEKVLYDLICSKQSMGIWIGD+KRETKY D++V
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120
           TKSLKALQ +G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLDREYI+TLKGF
Sbjct: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQI+EIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           FIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCTPNG+ISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

[Close]

BLAST of MS014085.1 vs. ExPASy TrEMBL
Match: A0A6J1D758 (DNA-directed RNA polymerase III subunit rpc6 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017972 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.9e-111
Identity = 198/199 (99.50%), Postives = 198/199 (99.50%), Query Frame = 0


Query: 40  MGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGG 99
           MGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGG
Sbjct: 1   MGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGG 60

Query: 100 NWYSEGKLDREYINTLKGFCARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNA 159
           NWYSEGKLDREYINTLKGFC RMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNA
Sbjct: 61  NWYSEGKLDREYINTLKGFCGRMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNA 120

Query: 160 LVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCT 219
           LVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCT
Sbjct: 121 LVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCT 180

Query: 220 PNGVISPRTCVYYTKWLDF 239
           PNGVISPRTCVYYTKWLDF
Sbjct: 181 PNGVISPRTCVYYTKWLDF 199

[Close]

BLAST of MS014085.1 vs. ExPASy TrEMBL
Match: A0A1S3BL06 (DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC103490992 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 2.1e-105
Identity = 191/243 (78.60%), Postives = 219/243 (90.12%), Query Frame = 0


Query: 1   MSRSQGPSSLKRKRPD----SSSGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADS 60
           MS+SQGPSSLKRKRPD    +SSGGALI  EKVLYDLICSKQSMGI   D+KRETK+A+S
Sbjct: 1   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 60

Query: 61  IVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLK 120
           +VTKSLK+LQ KG+IK+V+HYQNKGKKFYLS DFEP+KELTGGNWY +G+LD++YINTLK
Sbjct: 61  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 120

Query: 121 GFCARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGE 180
             CARMIQ+ K+ TIEGIV+SFK+SGA KV+LSREQI+EIVNALVLDN+VMEVKS+G GE
Sbjct: 121 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 180

Query: 181 FDFIPIGKICYK-CYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKW 239
           F+FIPIGKICYK C   GG+KGEPK GAMASIPCGVCPRI++CTPNG+ISP TCVYYTKW
Sbjct: 181 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 240

[Close]

BLAST of MS014085.1 vs. TAIR 10
Match: AT5G23710.1 (DNA binding;DNA-directed RNA polymerases )

HSP 1 Score: 207.2 bits (526), Expect = 1.4e-53
Identity = 113/232 (48.71%), Postives = 148/232 (63.79%), Query Frame = 0


Query: 11  KRKRPD-SSSGGALIE-HEKVLYDLICSKQSMGIWIGDIKRETKYADSIVTKSLKALQAK 70
           KRKRPD  SSGG L E HEK L DLI   Q  G  + ++KRE     +IVT+ + +L+ K
Sbjct: 3   KRKRPDPKSSGGDLNEPHEKKLLDLIRINQGRGATMFELKREKTIPATIVTRLIASLRKK 62

Query: 71  GMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGFCARMIQ--KM 130
            +IK V +  NKG K YL+ +FEP  ELTGG WY++G LD   I  LK  C  +++  + 
Sbjct: 63  NLIKEVANMNNKGVKHYLAMEFEPCSELTGGEWYTDGALDLSKIEDLKAKCVMILERHRQ 122

Query: 131 KVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFDFIPIGKIC 190
           +V T+E +   F K    +  LS +Q +EI+  L+LDN +MEVKS G  EF    IG++C
Sbjct: 123 RVVTLEVLCGYFVK----EEKLSVDQTKEILKNLILDNLIMEVKSNGMNEFASTRIGEVC 182

Query: 191 YKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 239
           Y+   K    GEP+ GA ASIPCGVCP I+ C+P+GVISP TCVY+ KWLDF
Sbjct: 183 YRLTGKKFGNGEPRAGAFASIPCGVCPHIAICSPDGVISPTTCVYFQKWLDF 230

The following BLAST results are available for this feature:

BLAST of MS014085.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149571.1	6.5e-133	99.58	DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >X...	[more]
XP_022970903.1	4.4e-121	90.00	DNA-directed RNA polymerase III subunit rpc6 [Cucurbita maxima]	[more]
KAG6604735.1	5.7e-121	90.00	DNA-directed RNA polymerase III subunit rpc6, partial [Cucurbita argyrosperma su...	[more]
XP_022946967.1	5.7e-121	90.00	DNA-directed RNA polymerase III subunit rpc6 [Cucurbita moschata] >XP_023532566....	[more]
XP_022149573.1	2.0e-110	99.50	DNA-directed RNA polymerase III subunit rpc6 isoform X2 [Momordica charantia] >X...	[more]

BLAST of MS014085.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q86JM3	1.0e-24	28.81	DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=4468...	[more]
Q2T9S3	1.5e-23	29.92	DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=...	[more]
Q9H1D9	3.4e-23	29.92	DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F P...	[more]
Q921X6	3.4e-23	30.33	DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f ...	[more]
P91529	2.9e-19	28.33	Probable DNA-directed RNA polymerase III subunit RPC6 OS=Caenorhabditis elegans ...	[more]

BLAST of MS014085.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D8D1	3.2e-133	99.58	DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia O...	[more]
A0A6J1I599	2.1e-121	90.00	DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1G553	2.8e-121	90.00	DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1D758	9.9e-111	99.50	DNA-directed RNA polymerase III subunit rpc6 isoform X2 OS=Momordica charantia O...	[more]
A0A1S3BL06	2.1e-105	78.60	DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC...	[more]

BLAST of MS014085.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23710.1	1.4e-53	48.71	DNA binding;DNA-directed RNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007832	RNA polymerase Rpc34	PFAM	PF05158	RNA_pol_Rpc34	coord: 23..127 e-value: 1.1E-25 score: 90.8
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 25..88 e-value: 5.9E-15 score: 57.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..15
IPR016049	RNA polymerase Rpc34-like	PANTHER	PTHR12780	RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED	coord: 17..238
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 25..87

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS014085	MS014085	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS014085.1-cds	MS014085.1-cds-scaffold5:476719..477432	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS014085.1	MS014085.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
cellular_component	GO:0005666	RNA polymerase III complex