MS013996.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS013996.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cysteine/Histidine-rich C1 domain family protein
Location	scaffold607: 1517122 .. 1518015 (+)
Sequence length	894
RNA-Seq Expression	MS013996.1
Synteny	MS013996.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACCGGCAACTCTACTCTCGCCCCATTTCTCCCAAGCCCATCCAAACCACCCACTGACGCATCACTCCGACGGCCGGGATTACACCTGCCACGCCTGCAACACCTCGGGTTTCGGCGCCCGATTTCAGTGCCACGCCTGCCCCGTCGACATCCACGTGTACTGCGCCAATTGCCCGGACGAATTGATCTCATCATTCCTCCACAGCCACCCCCTGACTCTGGCGCCTAGTACGGAGCGTCGGATCTGCGACGTCTGCCGCGGACCCATCGGCGGCATGCTGTACCGGTGCCAGGACTGCGGCTTCGACGTGCACCCACTCTGCACCCAGCTCCCCGAGGAGGTCCGCCACGTCATAGACCACACCCATAAGCTGAAGCTCAAGAAAGTGCCGTCTGGAAATTGCGCTGTTTGCGGATTCGATTGCTCTTCTCTCTGGGTCTACGGCTGCGATGTTTGTGTAGTCAACACCAATATCCATCCACAATGCCTTCTGAAGCCGTACCGGAGTGACACGTTACCCGTCCCGCCGGCCGGCTCACGCGGGATAAAACATGCGCCGCCACCTCATTTTCCCGGTGGGTTTTTTGGGCCGTATGGTTATGGTAATTATGATCCTAATTCTATGAATCTGAATGGTTTTGGTTTTGGATATGGAGGCGGCGGCTATTCTCCTCACGCCCATGTTTCTCCTTGGGGATTTCCATATTATTACCATTTCGCTCCTCCTCCATCGCCGATTTATTATGGACATGTTCCAAGTACTCATCAACCACATCATGGTGGCCATGGCCATACACCGCTGTCCATTGGGGGATTAATGTTTGGGAGCTCCATGTTTTCTCTGGTGGAGAATTTAGCGGCAGGTGCTATTACTGAGTTTCTATTCGGG

mRNA sequence

ATGGCACCGGCAACTCTACTCTCGCCCCATTTCTCCCAAGCCCATCCAAACCACCCACTGACGCATCACTCCGACGGCCGGGATTACACCTGCCACGCCTGCAACACCTCGGGTTTCGGCGCCCGATTTCAGTGCCACGCCTGCCCCGTCGACATCCACGTGTACTGCGCCAATTGCCCGGACGAATTGATCTCATCATTCCTCCACAGCCACCCCCTGACTCTGGCGCCTAGTACGGAGCGTCGGATCTGCGACGTCTGCCGCGGACCCATCGGCGGCATGCTGTACCGGTGCCAGGACTGCGGCTTCGACGTGCACCCACTCTGCACCCAGCTCCCCGAGGAGGTCCGCCACGTCATAGACCACACCCATAAGCTGAAGCTCAAGAAAGTGCCGTCTGGAAATTGCGCTGTTTGCGGATTCGATTGCTCTTCTCTCTGGGTCTACGGCTGCGATGTTTGTGTAGTCAACACCAATATCCATCCACAATGCCTTCTGAAGCCGTACCGGAGTGACACGTTACCCGTCCCGCCGGCCGGCTCACGCGGGATAAAACATGCGCCGCCACCTCATTTTCCCGGTGGGTTTTTTGGGCCGTATGGTTATGGTAATTATGATCCTAATTCTATGAATCTGAATGGTTTTGGTTTTGGATATGGAGGCGGCGGCTATTCTCCTCACGCCCATGTTTCTCCTTGGGGATTTCCATATTATTACCATTTCGCTCCTCCTCCATCGCCGATTTATTATGGACATGTTCCAAGTACTCATCAACCACATCATGGTGGCCATGGCCATACACCGCTGTCCATTGGGGGATTAATGTTTGGGAGCTCCATGTTTTCTCTGGTGGAGAATTTAGCGGCAGGTGCTATTACTGAGTTTCTATTCGGG

Coding sequence (CDS)

ATGGCACCGGCAACTCTACTCTCGCCCCATTTCTCCCAAGCCCATCCAAACCACCCACTGACGCATCACTCCGACGGCCGGGATTACACCTGCCACGCCTGCAACACCTCGGGTTTCGGCGCCCGATTTCAGTGCCACGCCTGCCCCGTCGACATCCACGTGTACTGCGCCAATTGCCCGGACGAATTGATCTCATCATTCCTCCACAGCCACCCCCTGACTCTGGCGCCTAGTACGGAGCGTCGGATCTGCGACGTCTGCCGCGGACCCATCGGCGGCATGCTGTACCGGTGCCAGGACTGCGGCTTCGACGTGCACCCACTCTGCACCCAGCTCCCCGAGGAGGTCCGCCACGTCATAGACCACACCCATAAGCTGAAGCTCAAGAAAGTGCCGTCTGGAAATTGCGCTGTTTGCGGATTCGATTGCTCTTCTCTCTGGGTCTACGGCTGCGATGTTTGTGTAGTCAACACCAATATCCATCCACAATGCCTTCTGAAGCCGTACCGGAGTGACACGTTACCCGTCCCGCCGGCCGGCTCACGCGGGATAAAACATGCGCCGCCACCTCATTTTCCCGGTGGGTTTTTTGGGCCGTATGGTTATGGTAATTATGATCCTAATTCTATGAATCTGAATGGTTTTGGTTTTGGATATGGAGGCGGCGGCTATTCTCCTCACGCCCATGTTTCTCCTTGGGGATTTCCATATTATTACCATTTCGCTCCTCCTCCATCGCCGATTTATTATGGACATGTTCCAAGTACTCATCAACCACATCATGGTGGCCATGGCCATACACCGCTGTCCATTGGGGGATTAATGTTTGGGAGCTCCATGTTTTCTCTGGTGGAGAATTTAGCGGCAGGTGCTATTACTGAGTTTCTATTCGGG

Protein sequence

MAPATLLSPHFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELISSFLHSHPLTLAPSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHVIDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPAGSRGIKHAPPPHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPWGFPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGHTPLSIGGLMFGSSMFSLVENLAAGAITEFLFG

Homology

[Close]

BLAST of MS013996.1 vs. NCBI nr
Match: XP_038883076.1 (diacylglycerol kinase theta-like [Benincasa hispida])

HSP 1 Score: 295.8 bits (756), Expect = 4.1e-76
Identity = 159/292 (54.45%), Postives = 182/292 (62.33%), Query Frame = 0


Query: 12  SQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELISSFLHSH 71
           SQ HPNH L HHSD RDYTCH C TSG G R++CHAC VD+H YC++ P EL SSFLH H
Sbjct: 15  SQVHPNHSLMHHSDDRDYTCHGCKTSGTGPRYRCHACNVDVHTYCSDSPQEL-SSFLHHH 74

Query: 72  PLTLAPSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHVIDHTHKLKLKKV 131
            LTL P  + R CD+CR  I GMLYRC  C FDVHPLCTQ PE+VRHVID  HKL L+K+
Sbjct: 75  RLTLVPRMDHRCCDICRESIDGMLYRCNRCDFDVHPLCTQFPEQVRHVIDDHHKLTLRKL 134

Query: 132 PSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPAGSRGI--KHAPP 191
            SG C+VC  DCSSLWVYGC+VC V  NIHP+CLLKPY S      P  SR I   HAPP
Sbjct: 135 SSGRCSVCEIDCSSLWVYGCNVCGV--NIHPKCLLKPYGS------PTKSRSIPYAHAPP 194

Query: 192 ---PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPWGFPYYYHFAPPPS 251
               H P G++        +PN         GYGGG +S     +PWGFP  +HF  PP 
Sbjct: 195 WAAAHQPNGYY-------VNPN---------GYGGGHFSHGGVPAPWGFP-NHHFNYPPG 254

Query: 252 PIYYGHVPSTHQPHHGGHGHTPLSIGGLMFGSSMFSLVENLAAGAITEFLFG 299
             Y G  PS              S  G +FG  MF+LV+NL AGAI EF+FG
Sbjct: 255 ANYGGRPPSP-------------STWGSIFGGGMFALVQNLTAGAIIEFIFG 267

[Close]

BLAST of MS013996.1 vs. NCBI nr
Match: KAG7033817.1 (hypothetical protein SDJN02_03542, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.9 bits (712), Expect = 5.2e-71
Identity = 160/308 (51.95%), Postives = 192/308 (62.34%), Query Frame = 0


Query: 7   LSPHFSQAHPNHPLTHH-SDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELIS 66
           L+PHFS  HPNH L H+ +D  +Y CHAC T+GFG RFQCH C  DIHVYCANCP +L S
Sbjct: 454 LNPHFSHVHPNHHLIHNTTDQANYICHACKTTGFGDRFQCHVCIADIHVYCANCPHQLSS 513

Query: 67  SFLHSHPLTL---APSTERR---ICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHV 126
           SF HSHPL+L    P T  R   ICD+CR  I G+ YRC  C FDVHPLCTQLPE+VRHV
Sbjct: 514 SF-HSHPLSLILSKPQTHHRTTTICDICRESIDGVSYRCSHCDFDVHPLCTQLPEKVRHV 573

Query: 127 IDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPA 186
           ID  H L+L+K   GNC VCG DC SLWVYGCD C V  N+H +CL K Y     P   +
Sbjct: 574 IDDYHSLRLQKSSFGNCGVCGIDCCSLWVYGCDGCRV--NVHIKCLRKSYG----PKKSS 633

Query: 187 GSRGIKHAPP------PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPW 246
           G+RGI HAPP      PHF GGF   YG GNY        G+GF +G     P+++    
Sbjct: 634 GTRGIPHAPPPSTAEKPHFNGGFPPGYG-GNY------YYGYGFPWG----VPNSYYGYP 693

Query: 247 GFPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGH---TPLSIGGLMFGSSMFSLVENLAAG 299
           G+P+ Y+   P  P Y+G        H  GHGH   +  S  G +FG++MFSL+ N+A+G
Sbjct: 694 GYPFPYNNNIPNYPPYHG------GEHGHGHGHGSSSSSSNWGSLFGTTMFSLIGNIASG 737

[Close]

BLAST of MS013996.1 vs. NCBI nr
Match: KAG6603634.1 (hypothetical protein SDJN03_04243, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278.9 bits (712), Expect = 5.2e-71
Identity = 160/308 (51.95%), Postives = 192/308 (62.34%), Query Frame = 0


Query: 7   LSPHFSQAHPNHPLTHH-SDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELIS 66
           L+PHFS  HPNH L H+ +D  +Y CHAC T+GFG RFQCH C  DIHVYCANCP +L S
Sbjct: 8   LNPHFSHVHPNHHLIHNTTDQANYICHACKTTGFGDRFQCHVCIADIHVYCANCPHQLSS 67

Query: 67  SFLHSHPLTL---APSTERR---ICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHV 126
           SF HSHPL+L    P T  R   ICD+CR  I G+ YRC  C FDVHPLCTQLPE+VRHV
Sbjct: 68  SF-HSHPLSLILSKPQTHHRTTTICDICRESIDGVSYRCSHCDFDVHPLCTQLPEKVRHV 127

Query: 127 IDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPA 186
           ID  H L+L+K   GNC VCG DC SLWVYGCD C V  N+H +CL K Y     P   +
Sbjct: 128 IDDYHSLRLQKSSFGNCGVCGIDCCSLWVYGCDGCRV--NVHIKCLRKSYG----PKKSS 187

Query: 187 GSRGIKHAPP------PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPW 246
           G+RGI HAPP      PHF GGF   YG GNY        G+GF +G     P+++    
Sbjct: 188 GTRGIPHAPPPSTAEKPHFNGGFPPGYG-GNY------YYGYGFPWG----VPNSYYGYP 247

Query: 247 GFPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGH---TPLSIGGLMFGSSMFSLVENLAAG 299
           G+P+ Y+   P  P Y+G        H  GHGH   +  S  G +FG++MFSL+ N+A+G
Sbjct: 248 GYPFPYNNNIPNYPPYHG------GEHGHGHGHGSSSSSSNWGSLFGTTMFSLIGNIASG 291

[Close]

BLAST of MS013996.1 vs. NCBI nr
Match: XP_022950372.1 (uncharacterized protein LOC111453485 [Cucurbita moschata])

HSP 1 Score: 278.1 bits (710), Expect = 8.9e-71
Identity = 159/308 (51.62%), Postives = 193/308 (62.66%), Query Frame = 0


Query: 7   LSPHFSQAHPNHPLTHH-SDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELIS 66
           L+PHFS  HPNH L H+ +D  +Y CHAC T+GFG RF+CH C  DIHVYCANCP +L S
Sbjct: 8   LNPHFSHVHPNHHLIHNTTDQANYICHACKTTGFGDRFRCHVCIADIHVYCANCPHQLSS 67

Query: 67  SFLHSHPLTL---APSTERR---ICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHV 126
           SF HSHPL+L    P T  R   ICD+CR  I G+ YRC  C FDVHPLCTQLPE+VRHV
Sbjct: 68  SF-HSHPLSLILSKPQTHHRTTTICDICRESIDGVSYRCSHCDFDVHPLCTQLPEKVRHV 127

Query: 127 IDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPA 186
           ID  H L+L+K   GNC VCG DC SLWVYGCD C V  N+H +CL K Y     P   +
Sbjct: 128 IDDYHSLRLQKSSFGNCGVCGIDCCSLWVYGCDGCRV--NVHIKCLRKSYG----PKKSS 187

Query: 187 GSRGIKHAPP------PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPW 246
           G+RGI HAPP      PHF GGF   +G GNY        G+GF +G     P+++    
Sbjct: 188 GTRGIPHAPPPSTAEKPHFNGGFPPGFG-GNY------YYGYGFRWG----VPNSYYGYP 247

Query: 247 GFPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGH---TPLSIGGLMFGSSMFSLVENLAAG 299
           G+P+ Y+   P  P Y+G        H  GHGH   +  S  G +FG++MFSL+ N+A+G
Sbjct: 248 GYPFPYNNNIPNYPPYHG------GEHGHGHGHGSSSSSSNWGSLFGTTMFSLIGNIASG 291

[Close]

BLAST of MS013996.1 vs. NCBI nr
Match: XP_023544024.1 (uncharacterized protein LOC111803731 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 277.3 bits (708), Expect = 1.5e-70
Identity = 159/308 (51.62%), Postives = 192/308 (62.34%), Query Frame = 0


Query: 7   LSPHFSQAHPNHPLTHH-SDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELIS 66
           L+PHFS  HPNH L H+ +D  +Y CHAC T+GFG RFQCH C  +IHVYCANCP +L S
Sbjct: 8   LNPHFSHVHPNHHLIHNTTDQANYICHACKTTGFGDRFQCHVCIAEIHVYCANCPHQLSS 67

Query: 67  SFLHSHPLTL---APSTERR---ICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHV 126
           SF HSHPL+L    P T  R   ICD+CR  I G+ YRC  C FDVHPLCTQLPE+VRHV
Sbjct: 68  SF-HSHPLSLILSKPQTHHRTTTICDICRESIDGVSYRCSHCDFDVHPLCTQLPEKVRHV 127

Query: 127 IDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPA 186
           ID  H L+L+K   GNC VCG DC SLWVYGCD C V  N+H +CL K Y     P   +
Sbjct: 128 IDDYHSLRLQKSSFGNCGVCGIDCCSLWVYGCDGCRV--NVHIKCLRKSYG----PKKSS 187

Query: 187 GSRGIKHAPP------PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPW 246
           G+RGI HAPP      PHF GGF   YG GNY        G+GF +G     P+++    
Sbjct: 188 GTRGIPHAPPPSTAEKPHFNGGFPPGYG-GNY------YYGYGFPWG----VPNSYYGYP 247

Query: 247 GFPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGH---TPLSIGGLMFGSSMFSLVENLAAG 299
           G+P+ Y+   P  P Y+G        H  GHGH   +  S  G +FG++MFSL+ N+A+G
Sbjct: 248 GYPFPYNNNIPNYPPYHG----GEHGHGHGHGHGSSSSSSNWGSLFGTTMFSLIGNIASG 293

[Close]

BLAST of MS013996.1 vs. ExPASy TrEMBL
Match: A0A6J1GEM0 (uncharacterized protein LOC111453485 OS=Cucurbita moschata OX=3662 GN=LOC111453485 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 4.3e-71
Identity = 159/308 (51.62%), Postives = 193/308 (62.66%), Query Frame = 0


Query: 7   LSPHFSQAHPNHPLTHH-SDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELIS 66
           L+PHFS  HPNH L H+ +D  +Y CHAC T+GFG RF+CH C  DIHVYCANCP +L S
Sbjct: 8   LNPHFSHVHPNHHLIHNTTDQANYICHACKTTGFGDRFRCHVCIADIHVYCANCPHQLSS 67

Query: 67  SFLHSHPLTL---APSTERR---ICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHV 126
           SF HSHPL+L    P T  R   ICD+CR  I G+ YRC  C FDVHPLCTQLPE+VRHV
Sbjct: 68  SF-HSHPLSLILSKPQTHHRTTTICDICRESIDGVSYRCSHCDFDVHPLCTQLPEKVRHV 127

Query: 127 IDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPA 186
           ID  H L+L+K   GNC VCG DC SLWVYGCD C V  N+H +CL K Y     P   +
Sbjct: 128 IDDYHSLRLQKSSFGNCGVCGIDCCSLWVYGCDGCRV--NVHIKCLRKSYG----PKKSS 187

Query: 187 GSRGIKHAPP------PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPW 246
           G+RGI HAPP      PHF GGF   +G GNY        G+GF +G     P+++    
Sbjct: 188 GTRGIPHAPPPSTAEKPHFNGGFPPGFG-GNY------YYGYGFRWG----VPNSYYGYP 247

Query: 247 GFPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGH---TPLSIGGLMFGSSMFSLVENLAAG 299
           G+P+ Y+   P  P Y+G        H  GHGH   +  S  G +FG++MFSL+ N+A+G
Sbjct: 248 GYPFPYNNNIPNYPPYHG------GEHGHGHGHGSSSSSSNWGSLFGTTMFSLIGNIASG 291

[Close]

BLAST of MS013996.1 vs. ExPASy TrEMBL
Match: A0A6J1IR43 (uncharacterized protein LOC111477694 OS=Cucurbita maxima OX=3661 GN=LOC111477694 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 7.4e-71
Identity = 161/314 (51.27%), Postives = 195/314 (62.10%), Query Frame = 0


Query: 7   LSPHFSQAHPNHPLTHH-SDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELIS 66
           L+PHFS  HPNH L H+ +D  +Y CHAC T+GFG RF+CH C  DIHVYCANCP +L S
Sbjct: 8   LNPHFSHVHPNHHLIHNTTDQTNYICHACKTTGFGDRFRCHVCIADIHVYCANCPHQLSS 67

Query: 67  SFLHSHPLTL---APSTERR---ICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHV 126
           SF HSHPL+L    P T  R   ICD+CR  I G+ YRC  C FDVHPLCTQLPE+VRHV
Sbjct: 68  SF-HSHPLSLILSKPQTHHRTTTICDICRESIDGVSYRCSHCDFDVHPLCTQLPEKVRHV 127

Query: 127 IDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPA 186
           ID  H L+L+K   GNC VCG DC SLWVYGCD C V  NIH +CL K Y S       +
Sbjct: 128 IDDYHSLRLQKSSFGNCGVCGIDCCSLWVYGCDGCRV--NIHIKCLRKSYGSK----KSS 187

Query: 187 GSRGIKHAPP------PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPW 246
           G+RGI++APP      PHF GGF   YG GNY        G+GF +G     P+++    
Sbjct: 188 GTRGIQNAPPPSTAEKPHFNGGFPPGYG-GNY------YYGYGFPWG----VPNSYYGYP 247

Query: 247 GFPYYYHFAPPPSPIY----YGHVPSTHQPHHGGHGH-----TPLSIGGLMFGSSMFSLV 299
           G+P+ Y+   P  P Y    +GH       H  GHGH     +  S  G +FG++MFSL+
Sbjct: 248 GYPFPYNNNIPNYPPYHGGGHGHGHGYGHGHGHGHGHGHESSSSSSNWGSLFGNTMFSLI 303

[Close]

BLAST of MS013996.1 vs. ExPASy TrEMBL
Match: A0A6J1DCG9 (uncharacterized protein LOC111019463 OS=Momordica charantia OX=3673 GN=LOC111019463 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 4.9e-67
Identity = 150/306 (49.02%), Postives = 183/306 (59.80%), Query Frame = 0


Query: 10  HFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELISSFLH 69
           HF+  HPNHPL HHSDG+DY C AC TSG G+RF+CHAC  ++H YCA+CP +L  SFLH
Sbjct: 11  HFT--HPNHPLLHHSDGQDYCCDACKTSGSGSRFRCHACDFNLHEYCADCPQKL-PSFLH 70

Query: 70  SHPLTLAPSTER------RICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHVIDHT 129
            HPL L     +      RICDVCRG + G+ YRC+DC FDVHPLCTQLP ++RHVID  
Sbjct: 71  RHPLALVVRKAQGARQNDRICDVCRGHVEGLFYRCKDCDFDVHPLCTQLPNQLRHVIDPN 130

Query: 130 HKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPA---- 189
           H L L K  S +CAVC  DCSSLWVYGCDVC    NIH  CLL+PY S + P P A    
Sbjct: 131 HSLNLHKPSSASCAVCKIDCSSLWVYGCDVC--RLNIHLDCLLQPYGSPSDPSPAAPPAS 190

Query: 190 --GSRGIKHAPPPHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPWGFPY 249
              SRGI  APPP +  G   P+ YG++         FG+GY      P     P+GFP+
Sbjct: 191 RCASRGIPFAPPPQWATG-PPPFSYGHF-------GNFGYGYAAPYGVPD---YPFGFPH 250

Query: 250 YYHFAPPPSPIYYGHVPSTHQPHHGGH-----GHTPLSIGGLMFGSSMFSLVENLAAGAI 299
           YYH +       YG+  + HQ   GG+        P +  G   G SMF+LV  L  G +
Sbjct: 251 YYHGS------MYGNA-NIHQGSQGGNVVGPPPPPPSASSGGKLGKSMFALVGKLTVGVM 293

[Close]

BLAST of MS013996.1 vs. ExPASy TrEMBL
Match: A0A5A7TQK1 (Diacylglycerol kinase theta-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00880 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 8.4e-67
Identity = 149/298 (50.00%), Postives = 175/298 (58.72%), Query Frame = 0


Query: 10  HFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELISSFLH 69
           HFS  HPNHPLTH+SD + YTCH CNT   G RF+C  C VDIH +C+N P+EL SSFLH
Sbjct: 9   HFSHFHPNHPLTHYSDDQKYTCHICNTVDSGLRFRCQPCHVDIHKFCSNSPEEL-SSFLH 68

Query: 70  SHPLTLAPSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHVIDHTHKLKLK 129
           SHPL L P    R CD+CR  I GM YRC  C FDVHPLCTQ PE++RH+ID  HKL L+
Sbjct: 69  SHPLALIPPMNHRQCDICRESINGMFYRCNHCDFDVHPLCTQFPEQLRHLIDGRHKLTLR 128

Query: 130 KVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPAGSRGIKH--A 189
           K+ SG C+VC  DCSS WVYGCDVC V  NIHP+C+L+PY S T      G+RGI +  A
Sbjct: 129 KLSSGRCSVCETDCSSFWVYGCDVCRV--NIHPRCILRPYGSST------GTRGISYWQA 188

Query: 190 PP---PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGG----GYSPHAHVSPWGFPYYYH 249
           PP    H P G+ G Y                F YGGG    GY P+ HV  + +   Y 
Sbjct: 189 PPWTTAHQPNGYGGGY----------------FAYGGGPANLGY-PNNHVG-YSYGGNYG 248

Query: 250 FAPPPSPIYYGHVPSTHQPHHGGHGHTPLSIGGLMFGSSMFSLVENLAAGAITEFLFG 299
             PP  P +   VP                    M G+ MF LV+NL AGAI EF+FG
Sbjct: 249 GQPPSRPNW---VP--------------------MLGAGMFGLVQNLTAGAIIEFIFG 256

[Close]

BLAST of MS013996.1 vs. ExPASy TrEMBL
Match: A0A1S3BK43 (diacylglycerol kinase theta-like OS=Cucumis melo OX=3656 GN=LOC103490570 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 8.4e-67
Identity = 149/298 (50.00%), Postives = 175/298 (58.72%), Query Frame = 0


Query: 10  HFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELISSFLH 69
           HFS  HPNHPLTH+SD + YTCH CNT   G RF+C  C VDIH +C+N P+EL SSFLH
Sbjct: 9   HFSHFHPNHPLTHYSDDQKYTCHICNTVDSGLRFRCQPCHVDIHKFCSNSPEEL-SSFLH 68

Query: 70  SHPLTLAPSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHVIDHTHKLKLK 129
           SHPL L P    R CD+CR  I GM YRC  C FDVHPLCTQ PE++RH+ID  HKL L+
Sbjct: 69  SHPLALIPPMNHRQCDICRESINGMFYRCNHCDFDVHPLCTQFPEQLRHLIDGRHKLTLR 128

Query: 130 KVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTLPVPPAGSRGIKH--A 189
           K+ SG C+VC  DCSS WVYGCDVC V  NIHP+C+L+PY S T      G+RGI +  A
Sbjct: 129 KLSSGRCSVCETDCSSFWVYGCDVCRV--NIHPRCILRPYGSST------GTRGISYWQA 188

Query: 190 PP---PHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGG----GYSPHAHVSPWGFPYYYH 249
           PP    H P G+ G Y                F YGGG    GY P+ HV  + +   Y 
Sbjct: 189 PPWTTAHQPNGYGGGY----------------FAYGGGPANLGY-PNNHVG-YSYGGNYG 248

Query: 250 FAPPPSPIYYGHVPSTHQPHHGGHGHTPLSIGGLMFGSSMFSLVENLAAGAITEFLFG 299
             PP  P +   VP                    M G+ MF LV+NL AGAI EF+FG
Sbjct: 249 GQPPSRPNW---VP--------------------MLGAGMFGLVQNLTAGAIIEFIFG 256

[Close]

BLAST of MS013996.1 vs. TAIR 10
Match: AT2G37820.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 139.8 bits (351), Expect = 3.5e-33
Identity = 99/314 (31.53%), Postives = 135/314 (42.99%), Query Frame = 0


Query: 1   MAPATLLSPHFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCP 60
           MAP TL           HPLT  +   D+ C  C T G G  ++C  C  D+H YCA CP
Sbjct: 1   MAPRTLKRHTVQHFTHIHPLTEFNSIGDFICDGCKTYGSGKTYRCEPCNYDLHEYCATCP 60

Query: 61  DELISSFLH-SHPLTLAPSTER------RICDVCRGPIGGMLYRCQDCGFDVHPLCTQLP 120
             L  +F+H  H L+L    ++      R CD+C   + G+ YRC+ C FDVHPLCTQLP
Sbjct: 61  LTL-PTFIHPQHELSLVVRKQQSTRQNDRACDICDESVEGLFYRCKICEFDVHPLCTQLP 120

Query: 121 EEVRHVIDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLL---KPYR 180
           + VRHV+   H L+ +   +  C VC   C S W Y C++C    +IH +C+L       
Sbjct: 121 QHVRHVLHPAHHLEFRPSGASTCMVCHGPCQS-WRYRCELC--RFDIHMECILAVCNTSP 180

Query: 181 SDTLPVPPAGSRGIKH-----APPPHFPGGFFG-PYGYGNYDPNSMNLNGFGFGYGGGGY 240
           SD        SRG+K      +PP  +    FG PY YG         N   + Y    Y
Sbjct: 181 SDQTDASGTKSRGLKQQGGQPSPPTQWQQPHFGYPYAYGPMGQQQPYFNYHPYNYNNFNY 240

Query: 241 SPHAHVSPWGFPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGHTPLSIGGLMFGSSMFSLV 299
               +++P                  G VP            TP        G  MF++ 
Sbjct: 241 MNGYNMNP------------------GQVPMA----GASQNSTPSK------GWRMFTIA 282

[Close]

BLAST of MS013996.1 vs. TAIR 10
Match: AT2G37800.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 118.2 bits (295), Expect = 1.1e-26
Identity = 88/304 (28.95%), Postives = 123/304 (40.46%), Query Frame = 0


Query: 3   PATLLSPHFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDE 62
           P  L+  HF+     HPLT      ++TC  C T GFG  ++C  C  ++H +CA CP  
Sbjct: 140 PEQLVVQHFTHI---HPLTKVDGYGEFTCDGCKTYGFGKTYRCTRCDYNLHDHCATCPST 199

Query: 63  LISSFLH-SHPLTLA------PSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEE 122
           L ++F+H  H L L           +R+CD+C     G+ Y+C+ CGFDVHPLCTQLP+ 
Sbjct: 200 L-ATFMHPQHELRLVFRGPEHTHQNKRMCDICDESAEGLYYQCEPCGFDVHPLCTQLPQH 259

Query: 123 VRHVIDHTHKLKLKK-VPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCLLKPYRSDTL 182
           VRHV    H L+L +   S  C VC     S W Y C  C    ++H +C+     S   
Sbjct: 260 VRHVPHPAHLLELSQWGASSICMVCRGAIRS-WRYKCGPC--GLDVHMECI----SSSAS 319

Query: 183 PVPPAGSRGIKHAPPPHFPGGFFGPYGYGNYDPNSMNLNGFGFGYGGGGYSPHAHVSPWG 242
                  +    +P P F                                        + 
Sbjct: 320 VAATKIQQRCFGSPQPQF----------------------------------------YH 379

Query: 243 FPYYYHFAPPPSPIYYGHVPSTHQPHHGGHGHTPLSIGGLMFGSSMFSLVENLAAGAITE 299
             YYYH A      YY H  +     HG      +   G   G  MFS++ +L  G +  
Sbjct: 380 SEYYYHHA------YYNHGYTNQGQVHGYANQGQVQEPGRSKGRKMFSILMDLTVGVVCN 386

[Close]

BLAST of MS013996.1 vs. TAIR 10
Match: AT2G37810.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-26
Identity = 59/157 (37.58%), Postives = 84/157 (53.50%), Query Frame = 0


Query: 10  HFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELISSFLH 69
           HFS     HPLT   +  ++TC  C T GFG  ++C  C   +H +CA CP  LIS    
Sbjct: 13  HFSH---THPLTKVDEYGEFTCGGCKTYGFGKTYRCAPCDYVLHDHCATCPFTLISFMHP 72

Query: 70  SHPLTLAPSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEEVRHVIDHTHKLKLK 129
            H L L  +    +C++C   + G+ Y C+ CGFDVHPLCTQ  + V +V    H L+L 
Sbjct: 73  QHELRLFVNGSENMCNICHRLVEGVYYHCETCGFDVHPLCTQHHQHVSYVPHPAHLLELS 132

Query: 130 KVPSGN-CAVCGFDCSSLWVYGCDVCVVNTNIHPQCL 166
           +  + N C VC     S W Y C  C++  ++H +C+
Sbjct: 133 QCGASNTCMVCRGAILS-WRYKCGPCML--DVHVECV 163

[Close]

BLAST of MS013996.1 vs. TAIR 10
Match: AT2G16050.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 86.7 bits (213), Expect = 3.5e-17
Identity = 54/170 (31.76%), Postives = 74/170 (43.53%), Query Frame = 0


Query: 10  HFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCH-----ACPVDIHVYCANCPDELI 69
           HFS  HP H L      + + C  CN  G G+R++C      +C  D+H +CA      +
Sbjct: 8   HFS--HPQHNLKFDYTEKPFKCDGCNEVGIGSRYRCSGDNHGSCDFDLHAHCA------L 67

Query: 70  SSFLHSHP---------LTLAPSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLPEE 129
            S   +HP         +   P  ERR C+ C   + G LY C+ CGFD+HP C  LP  
Sbjct: 68  PSASITHPFYKKCTFQFVARPPGNERRYCNACEKDVSGFLYHCKACGFDLHPCCAMLPMV 127

Query: 130 VRHVIDHTHKLKLKKVPSGNCAVCGFDCSSLWVYGCDVCVVNTNIHPQCL 166
           +    D    L L +  S +C  CG    S W Y         N+H  C+
Sbjct: 128 LD---DGETNLFLYRKVSSSCHRCGKKGRS-WSYRSS--CKKYNLHVACV 163

[Close]

BLAST of MS013996.1 vs. TAIR 10
Match: AT2G37780.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 86.3 bits (212), Expect = 4.6e-17
Identity = 47/108 (43.52%), Postives = 58/108 (53.70%), Query Frame = 0


Query: 10  HFSQAHPNHPLTHHSDGRDYTCHACNTSGFGARFQCHACPVDIHVYCANCPDELISSFLH 69
           HF+    NH LT  +    YTC  C   G G  ++C  C  D+H YCA CP  L++S  H
Sbjct: 9   HFTH---NHLLTQVNGIGTYTCDGCKLYGEGRTYRCSDCDYDLHEYCATCPSILLNS-CH 68

Query: 70  --SHPLTL--APSTERRICDVCRGPIGGMLYRCQDCGFDVHPLCTQLP 114
              H L+L     TER  C VCR  I GM Y+C+ C F+ HPLCT  P
Sbjct: 69  GPDHELSLFNGHMTERS-CYVCRVSIQGMFYKCRQCSFEAHPLCTYAP 111

The following BLAST results are available for this feature:

BLAST of MS013996.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883076.1	4.1e-76	54.45	diacylglycerol kinase theta-like [Benincasa hispida]	[more]
KAG7033817.1	5.2e-71	51.95	hypothetical protein SDJN02_03542, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6603634.1	5.2e-71	51.95	hypothetical protein SDJN03_04243, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022950372.1	8.9e-71	51.62	uncharacterized protein LOC111453485 [Cucurbita moschata]	[more]
XP_023544024.1	1.5e-70	51.62	uncharacterized protein LOC111803731 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS013996.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS013996.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GEM0	4.3e-71	51.62	uncharacterized protein LOC111453485 OS=Cucurbita moschata OX=3662 GN=LOC1114534...	[more]
A0A6J1IR43	7.4e-71	51.27	uncharacterized protein LOC111477694 OS=Cucurbita maxima OX=3661 GN=LOC111477694...	[more]
A0A6J1DCG9	4.9e-67	49.02	uncharacterized protein LOC111019463 OS=Momordica charantia OX=3673 GN=LOC111019...	[more]
A0A5A7TQK1	8.4e-67	50.00	Diacylglycerol kinase theta-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BK43	8.4e-67	50.00	diacylglycerol kinase theta-like OS=Cucumis melo OX=3656 GN=LOC103490570 PE=4 SV...	[more]

BLAST of MS013996.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37820.1	3.5e-33	31.53	Cysteine/Histidine-rich C1 domain family protein	[more]
AT2G37800.1	1.1e-26	28.95	Cysteine/Histidine-rich C1 domain family protein	[more]
AT2G37810.1	1.9e-26	37.58	Cysteine/Histidine-rich C1 domain family protein	[more]
AT2G16050.1	3.5e-17	31.76	Cysteine/Histidine-rich C1 domain family protein	[more]
AT2G37780.1	4.6e-17	43.52	Cysteine/Histidine-rich C1 domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004146	DC1	PFAM	PF03107	C1_2	coord: 14..57 e-value: 7.8E-7 score: 29.3 coord: 67..109 e-value: 2.2E-10 score: 40.7
None	No IPR available	GENE3D	3.30.60.20		coord: 75..112 e-value: 1.7E-7 score: 32.7
None	No IPR available	PANTHER	PTHR47841	DIACYLGLYCEROL KINASE THETA-LIKE-RELATED	coord: 13..298
None	No IPR available	PANTHER	PTHR47841:SF7	DIACYLGLYCEROL KINASE THETA-LIKE	coord: 13..298
None	No IPR available	SUPERFAMILY	57889	Cysteine-rich domain	coord: 40..118

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS013996	MS013996	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS013996.1-cds	MS013996.1-cds-scaffold607:1517122..1518015	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS013996.1	MS013996.1-protein	polypeptide