MS013991.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS013991.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein MIZU-KUSSEI 1
Location	scaffold607: 1486748 .. 1487500 (-)
Sequence length	753
RNA-Seq Expression	MS013991.1
Synteny	MS013991.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAAATCGACGCCCTACGTCGGTTCCTTCTTCCTTGTTTCTTTCCCCCCGCCGCCGGCGCAACCGCCACCGCCACCTCCGCCGCCCCCAAGAAAAGACTAAGCACCTCCCTTCGCGACGACATCGAAACCACCGCCGCCACCAAATGCGATCCAACCCAAGACCAAGATTCCCCCCAAGGATCTCCGGCCACCACTCCCGACAGCGCCACGCCGAAGTTCGCCGCCGCCGCCGTCATGGCCGCACCGCGCCCGTCGAAATCCATGGTCATCGGAACAATCTTCGGCAACCGGCGCGGGCACGTGTGGTTCTGCGTCCAACACGACCGCCTCCGGACCAAGCCGTTTCTTCTACTCGAGTTCCCGATTCTGACTCACCAACTCGTCAACGAAATGCGATTCGGACTCGTCCGAATCGCCCTGGAGTGCAGCCGGGTCGAGCTCGGGTTGTGCCCGCTCCGTTCGATACCCGTCTGGGGAATGTCCTGCAACGGCCGGAAACTCGGGTTCGCGGCGAGGAAAAAGGCCGGGGATCGGGTCCGATTCATGCTCAAGACTATGCAATCGACGACGGTCGGGGCGGGCGTTATTCCATCCGGATTCGGGTCGGGTTCGGAGGAGGTGATGTATATGAGAGCTAATTATGAGCACGTGGTGGGGAGTTCTGACTCGGAATCGTTTCATCTTATAAACCCGGACGAGTGCCCAGGTCAAGAACTCAGTATTTTCTTGCTCAGATCTCGAAATGGG

mRNA sequence

ATGGCCAAAATCGACGCCCTACGTCGGTTCCTTCTTCCTTGTTTCTTTCCCCCCGCCGCCGGCGCAACCGCCACCGCCACCTCCGCCGCCCCCAAGAAAAGACTAAGCACCTCCCTTCGCGACGACATCGAAACCACCGCCGCCACCAAATGCGATCCAACCCAAGACCAAGATTCCCCCCAAGGATCTCCGGCCACCACTCCCGACAGCGCCACGCCGAAGTTCGCCGCCGCCGCCGTCATGGCCGCACCGCGCCCGTCGAAATCCATGGTCATCGGAACAATCTTCGGCAACCGGCGCGGGCACGTGTGGTTCTGCGTCCAACACGACCGCCTCCGGACCAAGCCGTTTCTTCTACTCGAGTTCCCGATTCTGACTCACCAACTCGTCAACGAAATGCGATTCGGACTCGTCCGAATCGCCCTGGAGTGCAGCCGGGTCGAGCTCGGGTTGTGCCCGCTCCGTTCGATACCCGTCTGGGGAATGTCCTGCAACGGCCGGAAACTCGGGTTCGCGGCGAGGAAAAAGGCCGGGGATCGGGTCCGATTCATGCTCAAGACTATGCAATCGACGACGGTCGGGGCGGGCGTTATTCCATCCGGATTCGGGTCGGGTTCGGAGGAGGTGATGTATATGAGAGCTAATTATGAGCACGTGGTGGGGAGTTCTGACTCGGAATCGTTTCATCTTATAAACCCGGACGAGTGCCCAGGTCAAGAACTCAGTATTTTCTTGCTCAGATCTCGAAATGGG

Coding sequence (CDS)

ATGGCCAAAATCGACGCCCTACGTCGGTTCCTTCTTCCTTGTTTCTTTCCCCCCGCCGCCGGCGCAACCGCCACCGCCACCTCCGCCGCCCCCAAGAAAAGACTAAGCACCTCCCTTCGCGACGACATCGAAACCACCGCCGCCACCAAATGCGATCCAACCCAAGACCAAGATTCCCCCCAAGGATCTCCGGCCACCACTCCCGACAGCGCCACGCCGAAGTTCGCCGCCGCCGCCGTCATGGCCGCACCGCGCCCGTCGAAATCCATGGTCATCGGAACAATCTTCGGCAACCGGCGCGGGCACGTGTGGTTCTGCGTCCAACACGACCGCCTCCGGACCAAGCCGTTTCTTCTACTCGAGTTCCCGATTCTGACTCACCAACTCGTCAACGAAATGCGATTCGGACTCGTCCGAATCGCCCTGGAGTGCAGCCGGGTCGAGCTCGGGTTGTGCCCGCTCCGTTCGATACCCGTCTGGGGAATGTCCTGCAACGGCCGGAAACTCGGGTTCGCGGCGAGGAAAAAGGCCGGGGATCGGGTCCGATTCATGCTCAAGACTATGCAATCGACGACGGTCGGGGCGGGCGTTATTCCATCCGGATTCGGGTCGGGTTCGGAGGAGGTGATGTATATGAGAGCTAATTATGAGCACGTGGTGGGGAGTTCTGACTCGGAATCGTTTCATCTTATAAACCCGGACGAGTGCCCAGGTCAAGAACTCAGTATTTTCTTGCTCAGATCTCGAAATGGG

Protein sequence

MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSPQGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAGDRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQELSIFLLRSRNG

Homology

[Close]

BLAST of MS013991.1 vs. NCBI nr
Match: XP_022151581.1 (protein MIZU-KUSSEI 1 [Momordica charantia])

HSP 1 Score: 501.1 bits (1289), Expect = 5.4e-138
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP 60
           MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP 60

Query: 61  QGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLL 120
           QGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLL
Sbjct: 61  QGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLL 120

Query: 121 EFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAGDR 180
           EFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAGDR
Sbjct: 121 EFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAGDR 180

Query: 181 VRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQE 240
           VRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQE
Sbjct: 181 VRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQE 240

Query: 241 LSIFLLRSRNG 252
           LSIFLLRSRNG
Sbjct: 241 LSIFLLRSRNG 251

[Close]

BLAST of MS013991.1 vs. NCBI nr
Match: XP_038880832.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 431.4 bits (1108), Expect = 5.3e-117
Identity = 219/254 (86.22%), Postives = 231/254 (90.94%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKC--DPTQDQD 60
           MAKID+LRRFLLPCFFPP     ATA SA PKKRLSTSLRDD+ETT +T    DPT DQD
Sbjct: 1   MAKIDSLRRFLLPCFFPPTV-TNATAASAVPKKRLSTSLRDDVETTTSTTANGDPTNDQD 60

Query: 61  SPQGSPATTPDSATPKFAA-AAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPF 120
           S Q SPATTPD+ TPKFAA AA+ A PRPSK+MVIGTIFG+RRGHVWFCVQHDRLR KPF
Sbjct: 61  SAQDSPATTPDNVTPKFAATAAIAAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPF 120

Query: 121 LLLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKA 180
           LLLEFPILTHQLVNEMRFGLVRIALEC+RVELGLCPLRSIP+W MSCNGRKLGFAA+KKA
Sbjct: 121 LLLEFPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKA 180

Query: 181 GDRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECP 240
           GD VR MLKTMQSTTVGAGV+PSGFGSGSEEVMYMRANYEHVVGS+DSESFHLINPDECP
Sbjct: 181 GDSVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 240

Query: 241 GQELSIFLLRSRNG 252
           GQELSIFLLRSRNG
Sbjct: 241 GQELSIFLLRSRNG 253

[Close]

BLAST of MS013991.1 vs. NCBI nr
Match: XP_004142185.2 (protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017911 [Cucumis sativus])

HSP 1 Score: 421.8 bits (1083), Expect = 4.2e-114
Identity = 217/253 (85.77%), Postives = 231/253 (91.30%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAAT-KCDPTQDQDS 60
           MAKIDALRRFLLPCFFPP A ATA A+SA PKKRLSTSLRDD+ETT AT   DPT  QD 
Sbjct: 1   MAKIDALRRFLLPCFFPPTA-ATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQD- 60

Query: 61  PQGSPATTPDSATPKFA-AAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFL 120
              SPATTPDS TPKFA +A+++A PRPSK+MVIGTIFG+RRGHVWFCVQHDRLR KPFL
Sbjct: 61  ---SPATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALEC+RVELG CPLRSIP+W MSCNGRKLGFAA+KKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPG 240
           + VR MLKTMQSTTVGAGV+PSGFGSGSEEVMYMRANYEHVVGS+DSESFHLINPDECPG
Sbjct: 181 EPVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 252
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of MS013991.1 vs. NCBI nr
Match: XP_008448402.1 (PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0045148.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa] >TYK23589.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa])

HSP 1 Score: 418.3 bits (1074), Expect = 4.6e-113
Identity = 215/253 (84.98%), Postives = 229/253 (90.51%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKC-DPTQDQDS 60
           MAKIDALRRFLLPCFFPP A ATA A+S  PKKRLSTSLRDD+ETT AT   DPT  QD 
Sbjct: 1   MAKIDALRRFLLPCFFPPTA-ATAAASSTVPKKRLSTSLRDDLETTTATVSEDPTHAQD- 60

Query: 61  PQGSPATTPDSATPKFA-AAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFL 120
              SPATTPDS TPKFA +A+++A PRPSK+MVIGTIFG+RRGHVWFCVQHDRLR KPFL
Sbjct: 61  ---SPATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALEC+RVELG CPLRSIP+W MSCNGRKLGFAA+KKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPG 240
           + VR MLKTMQSTTVGAGV+PSG GSGSEEVMYMRANYEHVVGS+DSESFHLINPDECPG
Sbjct: 181 ESVRSMLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 252
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of MS013991.1 vs. NCBI nr
Match: XP_023517128.1 (protein MIZU-KUSSEI 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 406.8 bits (1044), Expect = 1.4e-109
Identity = 212/253 (83.79%), Postives = 226/253 (89.33%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP 60
           MAKIDALRR+LLPCFFPP   ATATA+SAAPKKRLSTSLRDD+ETT  T   PT DQD  
Sbjct: 1   MAKIDALRRYLLPCFFPPT--ATATASSAAPKKRLSTSLRDDVETT--TNGYPTDDQD-- 60

Query: 61  QGSPATTPDSATPKF--AAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFL 120
             SP+TTPDS TPKF  AA A++A PRPSK+MVIGTIFG+RRGH+WFCVQHDRLRTKPFL
Sbjct: 61  --SPSTTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LLE PILTHQLVNEMRFGLVRIALE + VELG CPLRSIP+W MSCNGRKLGFAARKK+G
Sbjct: 121 LLELPILTHQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKSG 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPG 240
           D VR MLKTMQSTTVGAGVIPSGFGS SEE+MYMRANYEHVVGS+DSESFHLINPDE PG
Sbjct: 181 DPVRSMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPG 240

Query: 241 QELSIFLLRSRNG 252
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 245

[Close]

BLAST of MS013991.1 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.7e-31
Identity = 98/254 (38.58%), Postives = 132/254 (51.97%), Query Frame = 0


Query: 10  FLLPC----FFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSPQGSPA 69
           FL+PC    + P ++ ++A+        + S  LR  I       C        P  S  
Sbjct: 50  FLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFINIINIPACKMLSLPSPPSSS-- 109

Query: 70  TTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLLEFPIL 129
               S+      + V          V GT++G++RGHV F VQ+++ R+ P LLL+  + 
Sbjct: 110 ----SSVSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMS 169

Query: 130 THQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG--DRVRF 189
           T  LV EM  GLVRIALEC +       L   P W M CNGRK G+A  +     D    
Sbjct: 170 TATLVKEMSSGLVRIALECEKRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWR 229

Query: 190 MLKTMQSTTVGAGVIP--------SGFGSGSE--EVMYMRANYEHVVGSSDSESFHLINP 248
           +L T+   TVGAGVIP        SG GSG+E  E++YMR  +E VVGS DSE+F+++NP
Sbjct: 230 VLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNP 289

[Close]

BLAST of MS013991.1 vs. ExPASy TrEMBL
Match: A0A6J1DF35 (protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111019486 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.6e-138
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP 60
           MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP 60

Query: 61  QGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLL 120
           QGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLL
Sbjct: 61  QGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLL 120

Query: 121 EFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAGDR 180
           EFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAGDR
Sbjct: 121 EFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAGDR 180

Query: 181 VRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQE 240
           VRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQE
Sbjct: 181 VRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQE 240

Query: 241 LSIFLLRSRNG 252
           LSIFLLRSRNG
Sbjct: 241 LSIFLLRSRNG 251

[Close]

BLAST of MS013991.1 vs. ExPASy TrEMBL
Match: A0A0A0KXC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.0e-114
Identity = 217/253 (85.77%), Postives = 231/253 (91.30%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAAT-KCDPTQDQDS 60
           MAKIDALRRFLLPCFFPP A ATA A+SA PKKRLSTSLRDD+ETT AT   DPT  QD 
Sbjct: 1   MAKIDALRRFLLPCFFPPTA-ATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQD- 60

Query: 61  PQGSPATTPDSATPKFA-AAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFL 120
              SPATTPDS TPKFA +A+++A PRPSK+MVIGTIFG+RRGHVWFCVQHDRLR KPFL
Sbjct: 61  ---SPATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALEC+RVELG CPLRSIP+W MSCNGRKLGFAA+KKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPG 240
           + VR MLKTMQSTTVGAGV+PSGFGSGSEEVMYMRANYEHVVGS+DSESFHLINPDECPG
Sbjct: 181 EPVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 252
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of MS013991.1 vs. ExPASy TrEMBL
Match: A0A5D3DK02 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001000 PE=4 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.2e-113
Identity = 215/253 (84.98%), Postives = 229/253 (90.51%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKC-DPTQDQDS 60
           MAKIDALRRFLLPCFFPP A ATA A+S  PKKRLSTSLRDD+ETT AT   DPT  QD 
Sbjct: 1   MAKIDALRRFLLPCFFPPTA-ATAAASSTVPKKRLSTSLRDDLETTTATVSEDPTHAQD- 60

Query: 61  PQGSPATTPDSATPKFA-AAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFL 120
              SPATTPDS TPKFA +A+++A PRPSK+MVIGTIFG+RRGHVWFCVQHDRLR KPFL
Sbjct: 61  ---SPATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALEC+RVELG CPLRSIP+W MSCNGRKLGFAA+KKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPG 240
           + VR MLKTMQSTTVGAGV+PSG GSGSEEVMYMRANYEHVVGS+DSESFHLINPDECPG
Sbjct: 181 ESVRSMLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 252
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of MS013991.1 vs. ExPASy TrEMBL
Match: A0A1S3BKH4 (protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103490601 PE=4 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.2e-113
Identity = 215/253 (84.98%), Postives = 229/253 (90.51%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKC-DPTQDQDS 60
           MAKIDALRRFLLPCFFPP A ATA A+S  PKKRLSTSLRDD+ETT AT   DPT  QD 
Sbjct: 1   MAKIDALRRFLLPCFFPPTA-ATAAASSTVPKKRLSTSLRDDLETTTATVSEDPTHAQD- 60

Query: 61  PQGSPATTPDSATPKFA-AAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFL 120
              SPATTPDS TPKFA +A+++A PRPSK+MVIGTIFG+RRGHVWFCVQHDRLR KPFL
Sbjct: 61  ---SPATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALEC+RVELG CPLRSIP+W MSCNGRKLGFAA+KKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPG 240
           + VR MLKTMQSTTVGAGV+PSG GSGSEEVMYMRANYEHVVGS+DSESFHLINPDECPG
Sbjct: 181 ESVRSMLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 252
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of MS013991.1 vs. ExPASy TrEMBL
Match: A0A6J1F0T8 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111438210 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 8.8e-110
Identity = 212/253 (83.79%), Postives = 225/253 (88.93%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSP 60
           MAKIDALRR+LLPCFFPP   ATATA+SAAPKKRLSTSLRDD+ETT  T   PT DQD  
Sbjct: 1   MAKIDALRRYLLPCFFPPT--ATATASSAAPKKRLSTSLRDDVETT--TNGYPTDDQD-- 60

Query: 61  QGSPATTPDSATPKF--AAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFL 120
             SP+TTPDS TPKF  AA A++A PRPSK+MVIGTIFG+RRGH+WFCVQHDRLRTKPFL
Sbjct: 61  --SPSTTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LLE PILTHQLVNEMRFGLVRIALE + VELG CPLRSIP+W MSCNGRKLGFAARKK G
Sbjct: 121 LLELPILTHQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKGG 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPG 240
           D VR MLKTMQSTTVGAGVIPSGFGS SEE+MYMRANYEHVVGS+DSESFHLINPDE PG
Sbjct: 181 DPVRSMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPG 240

Query: 241 QELSIFLLRSRNG 252
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 245

[Close]

BLAST of MS013991.1 vs. TAIR 10
Match: AT2G37880.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 280.0 bits (715), Expect = 1.8e-75
Identity = 155/256 (60.55%), Postives = 180/256 (70.31%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPAAGATATATS-AAPKKRLSTSLRDDIETTAATKCDPTQDQDS 60
           M KID+LRRFLLPC   P    T + TS  A KKRLSTSLRDDI+            QDS
Sbjct: 1   MTKIDSLRRFLLPCISTPTNPTTVSTTSTGASKKRLSTSLRDDIDV-----------QDS 60

Query: 61  PQGSPATTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLL 120
              S +++  ++   ++ +AV    RPSK+MVIGTIFG R+GHVWFCVQHDRL  KP LL
Sbjct: 61  ASSSASSSEATSAVDYSISAVTVPQRPSKTMVIGTIFGRRKGHVWFCVQHDRLSVKPILL 120

Query: 121 LEFPILTHQLVNEMRFGLVRIALEC-SRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG 180
           LE  I T QLV+EM  GLVR+ALEC +R EL  C LRS+PVW M CNGRKLGFA R+ A 
Sbjct: 121 LELSIATSQLVHEMGSGLVRVALECPTRPELKSCLLRSVPVWTMFCNGRKLGFAVRRSAN 180

Query: 181 DRVRFMLKTMQSTTVGAGVIPSGFGSG------SEEVMYMRANYEHVVGSSDSESFHLIN 240
           +  R MLK ++S TVGAGV+PSG G G      ++EVMYMRANYEHVVGSSDSESFHLIN
Sbjct: 181 EETRMMLKRLESMTVGAGVLPSGSGLGGSDESDTDEVMYMRANYEHVVGSSDSESFHLIN 240

Query: 241 PDECPGQELSIFLLRS 249
           PD    QELSIFLLR+
Sbjct: 241 PDANSAQELSIFLLRT 245

[Close]

BLAST of MS013991.1 vs. TAIR 10
Match: AT1G21050.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-36
Identity = 99/230 (43.04%), Postives = 131/230 (56.96%), Query Frame = 0


Query: 23  TATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSPQGSPATTPDSATPKFAAAAVMA 82
           T T+   + + R   +LR  IE      C  T  Q   Q    T   S+T   ++++  +
Sbjct: 23  TPTSVDCSREFRHRRTLRSLIECMIPYCC--TYQQQPYQND--TLSVSSTSSSSSSSDHS 82

Query: 83  APRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLLEFPILTHQLVNEM-RFGLVRIA 142
           +   S S V GT FG+RRG V FC+Q   + + P LLLE  + T  L  EM   G++RIA
Sbjct: 83  SSSQSSSTVSGTFFGHRRGRVSFCLQDAAVGSSPLLLLELAVPTAALAKEMDEEGVLRIA 142

Query: 143 LECSRVELGLCPLRSI---PVWGMSCNGRKLGFAARKKAGDRVRFMLKTMQSTTVGAGVI 202
           LEC R         SI   PVW M CNGRK+GFA R+K  +     L+ MQS +VGAGV+
Sbjct: 143 LECDRRRSSNSRSSSIFDVPVWSMFCNGRKMGFAVRRKVTENDAVFLRMMQSVSVGAGVV 202

Query: 203 PSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECPGQELSIFLLRS 249
           PS      ++++Y+RA +E V GSSDSESFH++NP    GQELSIFLLRS
Sbjct: 203 PS---EEEDQMLYLRARFERVTGSSDSESFHMMNPGGSYGQELSIFLLRS 245

[Close]

BLAST of MS013991.1 vs. TAIR 10
Match: AT1G76610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 144.4 bits (363), Expect = 1.2e-34
Identity = 79/160 (49.38%), Postives = 100/160 (62.50%), Query Frame = 0


Query: 90  MVIGTIFGNRRGHVWFCVQHD-RLRTKPFLLLEFPILTHQLVNEMRFGLVRIALECSRVE 149
           +V GT +G+RRGHV FC+Q D R  + P LLLE  + T  L  EM  G +RIAL      
Sbjct: 75  VVTGTFYGHRRGHVSFCLQDDTRPSSPPLLLLELAVPTAALAREMEEGFLRIALRSKSNR 134

Query: 150 LGLCPLRSIPVWGMSCNGRKLGFAARKKAGDRVRFMLKTMQSTTVGAGVIPSGFGSGSEE 209
                + ++PVW M CNGRK GFA R++  +     L+ MQS +VGAGVIP+G      E
Sbjct: 135 RS--SIFNVPVWSMYCNGRKFGFAVRRETTENDVGFLRLMQSVSVGAGVIPNG------E 194

Query: 210 VMYMRANYEHVVGSSDSESFHLINPDECPGQELSIFLLRS 249
            +Y+RA +E V GSSDSESFH++N     GQELSIFL RS
Sbjct: 195 TLYLRAKFERVTGSSDSESFHMVNQGGGYGQELSIFLSRS 226

[Close]

BLAST of MS013991.1 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 137.1 bits (344), Expect = 1.9e-32
Identity = 98/254 (38.58%), Postives = 132/254 (51.97%), Query Frame = 0


Query: 10  FLLPC----FFPPAAGATATATSAAPKKRLSTSLRDDIETTAATKCDPTQDQDSPQGSPA 69
           FL+PC    + P ++ ++A+        + S  LR  I       C        P  S  
Sbjct: 50  FLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFINIINIPACKMLSLPSPPSSS-- 109

Query: 70  TTPDSATPKFAAAAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPFLLLEFPIL 129
               S+      + V          V GT++G++RGHV F VQ+++ R+ P LLL+  + 
Sbjct: 110 ----SSVSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMS 169

Query: 130 THQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKAG--DRVRF 189
           T  LV EM  GLVRIALEC +       L   P W M CNGRK G+A  +     D    
Sbjct: 170 TATLVKEMSSGLVRIALECEKRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWR 229

Query: 190 MLKTMQSTTVGAGVIP--------SGFGSGSE--EVMYMRANYEHVVGSSDSESFHLINP 248
           +L T+   TVGAGVIP        SG GSG+E  E++YMR  +E VVGS DSE+F+++NP
Sbjct: 230 VLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNP 289

[Close]

BLAST of MS013991.1 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 134.8 bits (338), Expect = 9.5e-32
Identity = 78/170 (45.88%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 91  VIGTIFGNRRGHVWFCVQHDRLRTKPFLLLEFPILTHQLVNEMRFGLVRIALECSRVEL- 150
           V+GT+FGNRRGHV+F VQ D  R  P +L++ P  T  LV EM  GLVRIALE +  +  
Sbjct: 98  VVGTLFGNRRGHVYFAVQDDPTRL-PAVLIQLPTPTSVLVREMASGLVRIALETAAYKTD 157

Query: 151 GLCPLRSIPVWGMSCNGRKLGFAARKKAGDRVRFMLKTMQSTTVGAGVIPS--------- 210
               L     W   CNG+K G+AARK+ G+    +LK +   T+GAGV+P+         
Sbjct: 158 SKKKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEG 217

Query: 211 --GFGSGSEEVMYMRANYEHVVGSSDSESFHLINPD-ECPGQELSIFLLR 248
               GS   E+MYMRA +E VVGS DSE+F+++NPD    G ELS++ LR
Sbjct: 218 NGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLR 266

The following BLAST results are available for this feature:

BLAST of MS013991.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151581.1	5.4e-138	100.00	protein MIZU-KUSSEI 1 [Momordica charantia]	[more]
XP_038880832.1	5.3e-117	86.22	protein MIZU-KUSSEI 1 [Benincasa hispida]	[more]
XP_004142185.2	4.2e-114	85.77	protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017...	[more]
XP_008448402.1	4.6e-113	84.98	PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0045148.1 protein MIZU-KUSSE...	[more]
XP_023517128.1	1.4e-109	83.79	protein MIZU-KUSSEI 1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS013991.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	2.7e-31	38.58	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of MS013991.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DF35	2.6e-138	100.00	protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111019486 PE=4 SV=1	[more]
A0A0A0KXC2	2.0e-114	85.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1	[more]
A0A5D3DK02	2.2e-113	84.98	Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50...	[more]
A0A1S3BKH4	2.2e-113	84.98	protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103490601 PE=4 SV=1	[more]
A0A6J1F0T8	8.8e-110	83.79	protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111438210 PE=4 SV=1	[more]

BLAST of MS013991.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37880.1	1.8e-75	60.55	Protein of unknown function, DUF617	[more]
AT1G21050.1	1.3e-36	43.04	Protein of unknown function, DUF617	[more]
AT1G76610.1	1.2e-34	49.38	Protein of unknown function, DUF617	[more]
AT2G41660.1	1.9e-32	38.58	Protein of unknown function, DUF617	[more]
AT3G25640.1	9.5e-32	45.88	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 91..247 e-value: 1.9E-58 score: 197.2
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 91..248 e-value: 2.2E-58 score: 194.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..70
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 23..71
None	No IPR available	PANTHER	PTHR31276	FAMILY NOT NAMED	coord: 1..249
None	No IPR available	PANTHER	PTHR31276:SF6	PROTEIN MIZU-KUSSEI 1	coord: 1..249

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS013991	MS013991	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS013991.1-cds	MS013991.1-cds-scaffold607:1486748..1487500	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS013991.1	MS013991.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism