MS013577.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS013577.1 vs. NCBI nr
Match: XP_022140054.1 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 [Momordica charantia] >XP_022140055.1 probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X2 [Momordica charantia])

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 691/727 (95.05%), Postives = 692/727 (95.19%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV
Sbjct: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEA ERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAXERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQ DID+AIQLLREEERN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQGDIDSAIQLLREEERN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQELVFSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS
Sbjct: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY
Sbjct: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR
Sbjct: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 695

Query: 721 QLSHMPH 728
           QLSHMPH
Sbjct: 721 QLSHMPH 695

BLAST of MS013577.1 vs. NCBI nr
Match: XP_022140056.1 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X3 [Momordica charantia])

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 673/727 (92.57%), Postives = 674/727 (92.71%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV
Sbjct: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEA ERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAXERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQ DID+AIQLLREEERN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQGDIDSAIQLLREEERN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQELVFSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                                           ISDIESLIIAPISKASARQRTGRAGRIR
Sbjct: 361 --------------------------------ISDIESLIIAPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS
Sbjct: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY
Sbjct: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR
Sbjct: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 677

Query: 721 QLSHMPH 728
           QLSHMPH
Sbjct: 721 QLSHMPH 677

BLAST of MS013577.1 vs. NCBI nr
Match: XP_022955010.1 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 [Cucurbita moschata] >XP_022955011.1 probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1281.2 bits (3314), Expect = 0.0e+00
Identity = 660/727 (90.78%), Postives = 677/727 (93.12%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPG EKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYR+AILYLV
Sbjct: 1   MAQFWKPGAEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRSAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTN DVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNPDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAK+MSTFFQ SKRR   EGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKTMSTFFQTSKRRRGLEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQS VSTV+SIHEQEPPGDILVFLTGQDDID A+QLL E +RN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSAVSTVVSIHEQEPPGDILVFLTGQDDIDTAVQLLTENDRN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQEL+FSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELIFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYVVDSGFSKQRFYNPISDIE+LII PISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVVDSGFSKQRFYNPISDIENLIITPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFIN+MPAEGIPEMQRSNLV+ VIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINEMPAEGIPEMQRSNLVTCVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           +RALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASG+LGCSEEIITIAAVLS
Sbjct: 481 IRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGDLGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARL+FAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLQFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKV+EVREQLRRIAQRLGIVLKSCERDT  +RKAVTAGFFANA  LEAYSHNGMY
Sbjct: 601 NYQAMKKVMEVREQLRRIAQRLGIVLKSCERDTTVIRKAVTAGFFANACQLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           K+ RGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRN+ITIDPGWLTEAAPHFYQYR
Sbjct: 661 KSIRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNIITIDPGWLTEAAPHFYQYR 695

Query: 721 QLSHMPH 728
           QL+HMPH
Sbjct: 721 QLTHMPH 695

BLAST of MS013577.1 vs. NCBI nr
Match: XP_038894406.1 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 [Benincasa hispida])

HSP 1 Score: 1278.8 bits (3308), Expect = 0.0e+00
Identity = 662/727 (91.06%), Postives = 677/727 (93.12%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPGTEKP+LLDDGEGGVLFFSSSYSSSSGFGFSS EKQRQRLPVYKYRTAILYLV
Sbjct: 1   MAQFWKPGTEKPQLLDDGEGGVLFFSSSYSSSSGFGFSSTEKQRQRLPVYKYRTAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTN DVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNLDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQ SKRR   EGE LEPKV+PAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQTSKRRRGLEGEGLEPKVDPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVS+YVQS VSTVLSIHEQEPPGDILVFLTGQDDIDAA+QLLREEERN
Sbjct: 241 RGFNVQIFYLEEPVSDYVQSAVSTVLSIHEQEPPGDILVFLTGQDDIDAAVQLLREEERN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQEL+FSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELIFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYVVDSGFSKQRFYNPISDIESLI+APISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVVDSGFSKQRFYNPISDIESLIVAPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYF+N+MPAEGIPEMQRSNLV+ VIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFMNEMPAEGIPEMQRSNLVTCVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS
Sbjct: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIW SSRG QKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWASSRGGQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKV+EVREQLRR+AQRLGIVLK+ ERDT AVRKAVTAGFFANA  LEAYSHNGMY
Sbjct: 601 NYQAMKKVMEVREQLRRVAQRLGIVLKASERDTTAVRKAVTAGFFANACQLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           KT RGSQEVYIHPSSVLFRVNPKWV+YHSLVST+RQYMRNVITIDPGWLTEAAPHFYQ R
Sbjct: 661 KTVRGSQEVYIHPSSVLFRVNPKWVVYHSLVSTNRQYMRNVITIDPGWLTEAAPHFYQQR 695

Query: 721 QLSHMPH 728
           QLSHMP+
Sbjct: 721 QLSHMPY 695

BLAST of MS013577.1 vs. NCBI nr
Match: XP_022994348.1 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1278.8 bits (3308), Expect = 0.0e+00
Identity = 659/727 (90.65%), Postives = 676/727 (92.98%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPG EKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYR+AILYLV
Sbjct: 1   MAQFWKPGAEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRSAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LG EVGYTIRFEDLTN DVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM
Sbjct: 121 LGAEVGYTIRFEDLTNPDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAK+MSTFFQ SK+R   EGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKTMSTFFQTSKKRRGLEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQS VSTVLSIHEQEPPGDILVFLTGQDDID A+QLL E +RN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSAVSTVLSIHEQEPPGDILVFLTGQDDIDTAVQLLTEHDRN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQEL+FSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELIFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYVVDSGFSKQRFYNPISDIE+LII PISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVVDSGFSKQRFYNPISDIENLIITPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFIN+MPAEGIPEMQRSNLV+ VIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINEMPAEGIPEMQRSNLVTCVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           +RALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASG+LGCSEEIITIAAVLS
Sbjct: 481 IRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGDLGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARL+FAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLQFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKV+EVREQLRRIAQRLGIVLKSCERDT  +RKAVTAGFFANA  LEAYSHNGMY
Sbjct: 601 NYQAMKKVMEVREQLRRIAQRLGIVLKSCERDTTVIRKAVTAGFFANACQLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           K+ RGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRN+ITIDPGWLTEAAPHFYQYR
Sbjct: 661 KSIRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNIITIDPGWLTEAAPHFYQYR 695

Query: 721 QLSHMPH 728
           QL+HMPH
Sbjct: 721 QLTHMPH 695

BLAST of MS013577.1 vs. ExPASy Swiss-Prot
Match: F4JRJ6 (Probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 OS=Arabidopsis thaliana OX=3702 GN=At4g18465 PE=3 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 546/721 (75.73%), Postives = 627/721 (86.96%), Query Frame = 0

Query: 4   FWKPGTEKPRLLDDGEGGVLFFSSSY--SSSSGFGFSSIEKQRQRLPVYKYRTAILYLVE 63
           FWKPGTEKPR  +DGEGG++F S++   SSSS +G+++IEKQRQRLPVYKYRT ILYLVE
Sbjct: 3   FWKPGTEKPRFEEDGEGGIVFMSNNLASSSSSSYGYANIEKQRQRLPVYKYRTEILYLVE 62

Query: 64  THATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVKL 123
            HATTIIVGETGSGKTTQIPQYLKEAGWA+GGRVIACTQPRRLAVQAV++RVAEEMGV L
Sbjct: 63  NHATTIIVGETGSGKTTQIPQYLKEAGWAEGGRVIACTQPRRLAVQAVSARVAEEMGVNL 122

Query: 124 GEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDML 183
           GEEVGYTIRFED T + VT VKFLTDGVL+REMM+DPLLTKYSVIM+DEAHERSISTD+L
Sbjct: 123 GEEVGYTIRFEDHTTSGVTSVKFLTDGVLIREMMEDPLLTKYSVIMIDEAHERSISTDIL 182

Query: 184 LGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEGR 243
           LGLLKKIQRRRP+LRLIISSATIEAK+MS FF  SK+RH  EG    PK+EPAILSVEGR
Sbjct: 183 LGLLKKIQRRRPELRLIISSATIEAKTMSNFFNSSKKRHAPEGSTPGPKLEPAILSVEGR 242

Query: 244 GFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERND 303
           GF+V+I Y+EEPVS+Y++S VST+L I+E+EPPGD+LVFLTGQ+DI+ AI+LL EE  ++
Sbjct: 243 GFSVKIHYVEEPVSDYIRSVVSTILLINEREPPGDVLVFLTGQEDIETAIKLLEEEAHSN 302

Query: 304 RKKSE-LIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 363
           +K S  L+ LPLYSGLSR+EQEL+F+PT RGKRKV++STNIAETSLTLE           
Sbjct: 303 QKNSSGLLPLPLYSGLSRSEQELIFTPTPRGKRKVILSTNIAETSLTLE----------- 362

Query: 364 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 423
                         G+VYV+DSGFSKQ+FYNPISDIESL++APISKASARQR+GRAGR+R
Sbjct: 363 --------------GVVYVIDSGFSKQKFYNPISDIESLVVAPISKASARQRSGRAGRVR 422

Query: 424 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 483
           PGKCY RLYTE+YF+N MP EGIPEMQRSNLVS+VIQLKALGIDNILGFDWP+PPS E M
Sbjct: 423 PGKCY-RLYTEDYFLNQMPGEGIPEMQRSNLVSTVIQLKALGIDNILGFDWPAPPSSEAM 482

Query: 484 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 543
           +RALEVLYSL +LDDDAKLTSP GFQVAE+PL+PMISKMILAS ELGCS EIITIAAVLS
Sbjct: 483 IRALEVLYSLQILDDDAKLTSPTGFQVAELPLDPMISKMILASSELGCSHEIITIAAVLS 542

Query: 544 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 603
           +      QS+W  +RG QKE DEA+LRFAAAEGDHVTFLNVYKGFL+S K +QWC+KNF+
Sbjct: 543 V------QSVWIIARGVQKEQDEAKLRFAAAEGDHVTFLNVYKGFLESKKPTQWCYKNFL 602

Query: 604 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 663
           NYQ+MKKV+E+R+QL+RIA+RLGI LKSC+ D  AVRKAVTAGFFANA  LE +S NG+Y
Sbjct: 603 NYQSMKKVVEIRDQLKRIARRLGITLKSCDGDMEAVRKAVTAGFFANACRLEPHS-NGVY 662

Query: 664 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 722
           KT RGS+EVYIHPSSVLFRVNPKWV+Y S+VST+RQYMRNV+TI+P WLTE APHFYQ R
Sbjct: 663 KTIRGSEEVYIHPSSVLFRVNPKWVVYQSIVSTERQYMRNVVTINPSWLTEVAPHFYQNR 690

BLAST of MS013577.1 vs. ExPASy Swiss-Prot
Match: Q5RBD4 (Probable ATP-dependent RNA helicase DHX35 OS=Pongo abelii OX=9601 GN=DHX35 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.6e-188
Identity = 350/724 (48.34%), Postives = 477/724 (65.88%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRL-LDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYL 60
           + +FW+PGTE P + + +    +   S +    + +   SIE+QRQ+LPV+K R  ILYL
Sbjct: 7   LVKFWRPGTEGPGVSISEERQSLAENSGTTVVYNPYAALSIEQQRQKLPVFKLRNHILYL 66

Query: 61  VETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGV 120
           +E + T +IVGETG GK+TQIPQYL EAGW   GRV+  TQPRR+A   VA RVAEE G 
Sbjct: 67  IENYQTVVIVGETGCGKSTQIPQYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGA 126

Query: 121 KLGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTD 180
            LG EVGY IRF+D T+   TR+KFLTDG+L+REMM DPLLTKYSVIM+DEAHER++ TD
Sbjct: 127 VLGHEVGYCIRFDDCTDQLATRIKFLTDGMLVREMMVDPLLTKYSVIMLDEAHERTLYTD 186

Query: 181 MLLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEP-AILSV 240
           + +GLLKKIQ++R DLRLI++SAT++A     FF         + E  +P  +   IL+V
Sbjct: 187 IAIGLLKKIQKKRGDLRLIVASATLDADKFRDFFN--------QNETSDPARDTCVILTV 246

Query: 241 EGRGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEE 300
            GR F V IFYL+ PV +Y++STV TV+ IH+ E  GDIL FLTGQ++++  + +L E+ 
Sbjct: 247 GGRTFPVDIFYLQSPVPDYIKSTVETVVKIHQTEGDGDILAFLTGQEEVETVVSMLIEQA 306

Query: 301 R---NDRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFI 360
           R       K  L VLP+Y+GL   EQ  VF   +R  RKV+++TN+AETS+T        
Sbjct: 307 RALARTGMKRHLRVLPMYAGLPSFEQMKVFERVSRSVRKVIVATNVAETSIT-------- 366

Query: 361 KRLSYSLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGR 420
                            + GIVYV+D GF K R YNP + IE L++ P+S+ASA QR GR
Sbjct: 367 -----------------ISGIVYVIDCGFVKLRAYNPRTAIECLVVVPVSQASANQRAGR 426

Query: 421 AGRIRPGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPP 480
            GR R GKCY RLYTEE F + +P   +PEMQRSNL   ++QLKALGIDN+L F + SPP
Sbjct: 427 GGRSRSGKCY-RLYTEEAF-DKLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSPP 486

Query: 481 SPEVMVRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITI 540
             + MV+ALE+LY+LG LD D +LT P+G ++AE PL PM +KM+L SG  GCS+EI++I
Sbjct: 487 PAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEILSI 546

Query: 541 AAVLSIQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWC 600
           AA++ I      Q+I+      + +      +FA  EGDH+T LNVY+ F++ NK+SQWC
Sbjct: 547 AAMMQI------QNIFVVPPNQKSQAIRVHRKFAVEEGDHLTMLNVYEAFIKHNKNSQWC 606

Query: 601 HKNFINYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYS 660
            ++F+NY+ + +   VREQL+++  +  +  KS E D   V + + +GFFANA+    + 
Sbjct: 607 QEHFLNYKGLVRAATVREQLKKLLVKFQVPKKSSEGDPDPVLRCIVSGFFANAAR---FH 666

Query: 661 HNGMYKTTRGSQEVYIHPSSVLFRVN-PKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAP 719
             G Y+T R   E++IHP+SVL+    P+WVIY+ ++ T + YMR+V  I+  WL E AP
Sbjct: 667 STGAYRTIRDDHELHIHPASVLYAEKPPRWVIYNEVIQTSKYYMRDVTAIESAWLLELAP 686

BLAST of MS013577.1 vs. ExPASy Swiss-Prot
Match: Q9H5Z1 (Probable ATP-dependent RNA helicase DHX35 OS=Homo sapiens OX=9606 GN=DHX35 PE=1 SV=2)

HSP 1 Score: 659.1 bits (1699), Expect = 5.9e-188
Identity = 348/722 (48.20%), Postives = 475/722 (65.79%), Query Frame = 0

Query: 3   QFWKPGTEKPRL-LDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLVE 62
           +FW+PGTE P + + +    +   S +    + +   SIE+QRQ+LPV+K R  ILYL+E
Sbjct: 9   KFWRPGTEGPGVSISEERQSLAENSGTTVVYNPYAALSIEQQRQKLPVFKLRNHILYLIE 68

Query: 63  THATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVKL 122
            + T +IVGETG GK+TQIPQYL EAGW   GRV+  TQPRR+A   VA RVAEE G  L
Sbjct: 69  NYQTVVIVGETGCGKSTQIPQYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGAVL 128

Query: 123 GEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDML 182
           G EVGY IRF+D T+   TR+KFLTDG+L+REMM DPLLTKYSVIM+DEAHER++ TD+ 
Sbjct: 129 GHEVGYCIRFDDCTDQLATRIKFLTDGMLVREMMVDPLLTKYSVIMLDEAHERTLYTDIA 188

Query: 183 LGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEP-AILSVEG 242
           +GLLKKIQ++R DLRLI++SAT++A     FF         + E  +P  +   IL+VEG
Sbjct: 189 IGLLKKIQKKRGDLRLIVASATLDADKFRDFFN--------QNETSDPARDTCVILTVEG 248

Query: 243 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEER- 302
           R F V IFYL+ PV +Y++STV TV+ IH+ E  GD+L FLTGQ++++  + +L E+ R 
Sbjct: 249 RTFPVDIFYLQSPVPDYIKSTVETVVKIHQTEGDGDVLAFLTGQEEVETVVSMLIEQARA 308

Query: 303 --NDRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKR 362
                 K  L VLP+Y+GL   EQ  VF   +R  RKV+++TN+AETS+T          
Sbjct: 309 LARTGMKRHLRVLPMYAGLPSFEQMKVFERVSRSVRKVIVATNVAETSIT---------- 368

Query: 363 LSYSLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAG 422
                          + GIVYV+D GF K R YNP + IE L++ P+S+ASA QR GR G
Sbjct: 369 ---------------ISGIVYVIDCGFVKLRAYNPRTAIECLVVVPVSQASANQRAGRGG 428

Query: 423 RIRPGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSP 482
           R R GKCY RLYTEE F + +P   +PEMQRSNL   ++QLKALGIDN+L F + SPP  
Sbjct: 429 RSRSGKCY-RLYTEEAF-DKLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSPPPA 488

Query: 483 EVMVRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAA 542
           + MV+ALE+LY+LG LD D +LT P+G ++AE PL PM +KM+L SG  GCS+EI++IAA
Sbjct: 489 QSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEILSIAA 548

Query: 543 VLSIQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHK 602
           ++ I      Q+I+      +        +FA  EGDH+T LN+Y+ F++ NK S+WC +
Sbjct: 549 MMQI------QNIFVVPPNQKSHAIRVHRKFAVEEGDHLTMLNIYEAFIKHNKDSKWCQE 608

Query: 603 NFINYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHN 662
           +F+NY+ + +   VREQL+++  +  +  KS E D   V + + +GFFANA+    +   
Sbjct: 609 HFLNYKGLVRAATVREQLKKLLVKFQVPRKSSEGDPDLVLRCIVSGFFANAAR---FHST 668

Query: 663 GMYKTTRGSQEVYIHPSSVLFRVN-PKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHF 719
           G Y+T R   E++IHP+SVL+    P+WVIY+ ++ T + YMR+V  I+  WL E APHF
Sbjct: 669 GAYRTIRDDHELHIHPASVLYAEKPPRWVIYNEVIQTSKYYMRDVTAIESAWLLELAPHF 686

BLAST of MS013577.1 vs. ExPASy Swiss-Prot
Match: Q54F05 (ATP-dependent RNA helicase dhx8 OS=Dictyostelium discoideum OX=44689 GN=dhx8 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 8.6e-171
Identity = 321/681 (47.14%), Postives = 457/681 (67.11%), Query Frame = 0

Query: 39   SIEKQRQRLPVYKYRTAILYLVETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIAC 98
            SI++QR+ LP++  R A L  V  H   +++GETGSGKTTQ+ QYL EAG+   G+ I C
Sbjct: 500  SIKEQRESLPIFPLREAFLQAVSEHQLLVVIGETGSGKTTQMAQYLAEAGYGTRGK-IGC 559

Query: 99   TQPRRLAVQAVASRVAEEMGVKLGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDP 158
            TQPRR+A  +V+ RVAEE G +LG+EVGY IRFED T+ + T +KF+TDG+LLRE + DP
Sbjct: 560  TQPRRVAAMSVSKRVAEEFGCQLGQEVGYAIRFEDCTSPE-TIIKFMTDGILLRECLLDP 619

Query: 159  LLTKYSVIMVDEAHERSISTDMLLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKR 218
             L+ YSVI++DEAHER+ISTD+L GLLK+  +RRP+L+++I+SAT+EA+  S +F  ++ 
Sbjct: 620  NLSAYSVIILDEAHERTISTDVLFGLLKQALQRRPELKVLITSATLEAEKFSKYFMNAQ- 679

Query: 219  RHRFEGEALEPKVEPAILSVEGRGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDIL 278
                            +  + GR F V I Y ++P ++Y+ +++ TV+ IH  EPPGDIL
Sbjct: 680  ----------------LFIIPGRTFPVDIRYTKDPEADYLDASLITVMQIHLSEPPGDIL 739

Query: 279  VFLTGQDDIDAAIQLLREEERN-DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVI 338
            +FLTGQ++IDAA Q+L E  ++      +LI+LP+YS L    Q  +F P   G RKVVI
Sbjct: 740  LFLTGQEEIDAACQILYERMKSLGSNVPDLIILPVYSALPSEMQTKIFEPAPPGSRKVVI 799

Query: 339  STNIAETSLTLEVRFGFIKRLSYSLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIE 398
            +TNIAETSLT++                         GI YV+D GFSKQ+ +NP + ++
Sbjct: 800  ATNIAETSLTID-------------------------GIYYVIDPGFSKQKCFNPKNGMD 859

Query: 399  SLIIAPISKASARQRTGRAGRIRPGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQ 458
            SL++APIS+A+ARQR+GRAGR  PGKCY RLYTE  F N+M A  IPE+QR+NL ++V+ 
Sbjct: 860  SLVVAPISQAAARQRSGRAGRTGPGKCY-RLYTESAFKNEMLASSIPEIQRTNLGNTVLT 919

Query: 459  LKALGIDNILGFDWPSPPSPEVMVRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMIS 518
            +KA+GI+++L FD+  PP  + +V A+E LYSLG LD++  LT  +G ++AE PL+P +S
Sbjct: 920  MKAMGINDLLNFDFMDPPPVQTLVSAMEQLYSLGALDEEGLLTR-LGRKMAEFPLDPQLS 979

Query: 519  KMILASGELGCSEEIITIAAVLSIQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVT 578
            KM++AS +LGCS+EI+T+ A+LS+      Q+++   +  Q   D+ + +F   EGDH+T
Sbjct: 980  KMLIASVDLGCSDEILTVVAMLSV------QNVFYRPKEKQALADQKKAKFFQPEGDHLT 1039

Query: 579  FLNVYKGFLQSNKSSQWCHKNFINYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVR 638
             LNVY+ +  S  S+ WC +NF+  +++++  +VR+QL  I  R  + + S  R+   ++
Sbjct: 1040 LLNVYESWKNSKFSNPWCFENFVQARSLRRAQDVRKQLITIMDRYKLDIISAGRNYTKIQ 1099

Query: 639  KAVTAGFFANASHLEAYSHNGMYKTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQY 698
            KA+ +GFFANAS  +    N  YKT    Q VYIHPSS LF  NP WVIYH LV T ++Y
Sbjct: 1100 KAICSGFFANASKKDP---NEGYKTLVEGQPVYIHPSSTLFNRNPDWVIYHELVMTTKEY 1125

Query: 699  MRNVITIDPGWLTEAAPHFYQ 719
            MR V TIDP WL E AP F++
Sbjct: 1160 MREVCTIDPKWLVELAPKFFK 1125

BLAST of MS013577.1 vs. ExPASy Swiss-Prot
Match: Q8VY00 (Pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1 OS=Arabidopsis thaliana OX=3702 GN=ESP3 PE=1 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 5.8e-167
Identity = 318/685 (46.42%), Postives = 451/685 (65.84%), Query Frame = 0

Query: 40   IEKQRQRLPVYKYRTAILYLVETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACT 99
            +++ R+ LP+Y YR  +L  VE H   +IVG+TGSGKTTQIPQYL EAG+   G+V  CT
Sbjct: 397  LQEVRRSLPIYTYRDQLLKAVEEHQVLVIVGDTGSGKTTQIPQYLHEAGYTKRGKV-GCT 456

Query: 100  QPRRLAVQAVASRVAEEMGVKLGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPL 159
            QPRR+A  +VA+RVA+EMGVKLG EVGY+IRFED T +D T +K++TDG+LLRE++ +P 
Sbjct: 457  QPRRVAAMSVAARVAQEMGVKLGHEVGYSIRFEDCT-SDKTVLKYMTDGMLLRELLGEPD 516

Query: 160  LTKYSVIMVDEAHERSISTDMLLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRR 219
            L  YSV++VDEAHER++STD+L GL+K I R RPDL+L+ISSAT++A+  S +F  +   
Sbjct: 517  LASYSVVIVDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAEKFSDYFDTA--- 576

Query: 220  HRFEGEALEPKVEPAILSVEGRGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILV 279
                           I S  GR + V+I Y   P ++Y+ + + T+L+IH +EP GDILV
Sbjct: 577  --------------PIFSFPGRRYPVEINYTSAPEADYMDAAIVTILTIHVREPLGDILV 636

Query: 280  FLTGQDDIDAAIQLLREEERN-DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVIS 339
            F TGQ++I+ A ++L+   R    K  ELI+ P+Y+ L    Q  +F PT  G RKVV++
Sbjct: 637  FFTGQEEIETAEEILKHRIRGLGTKIRELIICPIYANLPSELQAKIFEPTPEGARKVVLA 696

Query: 340  TNIAETSLTLEVRFGFIKRLSYSLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIES 399
            TNIAETSLT++                         GI YVVD GFSK + YNP + +ES
Sbjct: 697  TNIAETSLTID-------------------------GIKYVVDPGFSKMKSYNPRTGMES 756

Query: 400  LIIAPISKASARQRTGRAGRIRPGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQL 459
            L+I PISKASA QR GRAGR  PGKCY RLYT   + ND+    +PE+QR+NL S V+ L
Sbjct: 757  LLITPISKASATQRAGRAGRTSPGKCY-RLYTAFNYNNDLEENTVPEVQRTNLASVVLAL 816

Query: 460  KALGIDNILGFDWPSPPSPEVMVRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISK 519
            K+LGI +++ FD+  PP  E +V++LE+L++LG L+   +LT   G ++AE PL+PM+SK
Sbjct: 817  KSLGIHDLINFDFMDPPPAEALVKSLELLFALGALNKLGELTK-AGRRMAEFPLDPMLSK 876

Query: 520  MILASGELGCSEEIITIAAVLSIQANVILQSIWTSSRGAQKELDEARLRFAAAE-GDHVT 579
            MI+ S +  CS+EII+IAA+LSI       SI+   +  Q   D AR+ F     GDH+ 
Sbjct: 877  MIVVSDKYKCSDEIISIAAMLSIGG-----SIFYRPKDKQVHADNARMNFHTGNVGDHIA 936

Query: 580  FLNVYKGFLQSNKSSQWCHKNFINYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVR 639
             L VY  + ++N S+QWC++N+I  ++MK+  ++R+QL  + +R+ I + S   +  +VR
Sbjct: 937  LLKVYSSWKETNFSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEIDISSNLNELDSVR 996

Query: 640  KAVTAGFFANASHLEAYSHNGMYKTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQY 699
            K++ AGFF + + L+    NG Y+T +  Q V+IHP+S L +V P+WV+YH LV T ++Y
Sbjct: 997  KSIVAGFFPHTAKLQ---KNGSYRTVKHPQTVHIHPNSGLSQVLPRWVVYHELVLTSKEY 1027

Query: 700  MRNVITIDPGWLTEAAPHFYQYRQL 723
            MR V  + P WL E APH+YQ + +
Sbjct: 1057 MRQVTELKPEWLIELAPHYYQLKDV 1027

BLAST of MS013577.1 vs. ExPASy TrEMBL
Match: A0A6J1CE11 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010804 PE=4 SV=1)

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 691/727 (95.05%), Postives = 692/727 (95.19%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV
Sbjct: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEA ERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAXERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQ DID+AIQLLREEERN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQGDIDSAIQLLREEERN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQELVFSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS
Sbjct: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY
Sbjct: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR
Sbjct: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 695

Query: 721 QLSHMPH 728
           QLSHMPH
Sbjct: 721 QLSHMPH 695

BLAST of MS013577.1 vs. ExPASy TrEMBL
Match: A0A6J1CEL4 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010804 PE=4 SV=1)

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 673/727 (92.57%), Postives = 674/727 (92.71%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV
Sbjct: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEA ERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAXERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQ DID+AIQLLREEERN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQGDIDSAIQLLREEERN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQELVFSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                                           ISDIESLIIAPISKASARQRTGRAGRIR
Sbjct: 361 --------------------------------ISDIESLIIAPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS
Sbjct: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY
Sbjct: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR
Sbjct: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 677

Query: 721 QLSHMPH 728
           QLSHMPH
Sbjct: 721 QLSHMPH 677

BLAST of MS013577.1 vs. ExPASy TrEMBL
Match: A0A6J1GTZ5 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457095 PE=4 SV=1)

HSP 1 Score: 1281.2 bits (3314), Expect = 0.0e+00
Identity = 660/727 (90.78%), Postives = 677/727 (93.12%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPG EKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYR+AILYLV
Sbjct: 1   MAQFWKPGAEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRSAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTN DVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNPDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAK+MSTFFQ SKRR   EGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKTMSTFFQTSKRRRGLEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQS VSTV+SIHEQEPPGDILVFLTGQDDID A+QLL E +RN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSAVSTVVSIHEQEPPGDILVFLTGQDDIDTAVQLLTENDRN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQEL+FSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELIFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYVVDSGFSKQRFYNPISDIE+LII PISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVVDSGFSKQRFYNPISDIENLIITPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFIN+MPAEGIPEMQRSNLV+ VIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINEMPAEGIPEMQRSNLVTCVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           +RALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASG+LGCSEEIITIAAVLS
Sbjct: 481 IRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGDLGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARL+FAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLQFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKV+EVREQLRRIAQRLGIVLKSCERDT  +RKAVTAGFFANA  LEAYSHNGMY
Sbjct: 601 NYQAMKKVMEVREQLRRIAQRLGIVLKSCERDTTVIRKAVTAGFFANACQLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           K+ RGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRN+ITIDPGWLTEAAPHFYQYR
Sbjct: 661 KSIRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNIITIDPGWLTEAAPHFYQYR 695

Query: 721 QLSHMPH 728
           QL+HMPH
Sbjct: 721 QLTHMPH 695

BLAST of MS013577.1 vs. ExPASy TrEMBL
Match: A0A6J1K4X6 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490096 PE=4 SV=1)

HSP 1 Score: 1278.8 bits (3308), Expect = 0.0e+00
Identity = 659/727 (90.65%), Postives = 676/727 (92.98%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPG EKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYR+AILYLV
Sbjct: 1   MAQFWKPGAEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRSAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LG EVGYTIRFEDLTN DVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM
Sbjct: 121 LGAEVGYTIRFEDLTNPDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAK+MSTFFQ SK+R   EGEALEPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKTMSTFFQTSKKRRGLEGEALEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVSNYVQS VSTVLSIHEQEPPGDILVFLTGQDDID A+QLL E +RN
Sbjct: 241 RGFNVQIFYLEEPVSNYVQSAVSTVLSIHEQEPPGDILVFLTGQDDIDTAVQLLTEHDRN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRKKSELIVLPLYSGLSRAEQEL+FSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKKSELIVLPLYSGLSRAEQELIFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYVVDSGFSKQRFYNPISDIE+LII PISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVVDSGFSKQRFYNPISDIENLIITPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFIN+MPAEGIPEMQRSNLV+ VIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINEMPAEGIPEMQRSNLVTCVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           +RALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASG+LGCSEEIITIAAVLS
Sbjct: 481 IRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGDLGCSEEIITIAAVLS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIWTSSRGAQKELDEARL+FAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWTSSRGAQKELDEARLQFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKV+EVREQLRRIAQRLGIVLKSCERDT  +RKAVTAGFFANA  LEAYSHNGMY
Sbjct: 601 NYQAMKKVMEVREQLRRIAQRLGIVLKSCERDTTVIRKAVTAGFFANACQLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           K+ RGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRN+ITIDPGWLTEAAPHFYQYR
Sbjct: 661 KSIRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNIITIDPGWLTEAAPHFYQYR 695

Query: 721 QLSHMPH 728
           QL+HMPH
Sbjct: 721 QLTHMPH 695

BLAST of MS013577.1 vs. ExPASy TrEMBL
Match: A0A1S3B6Y9 (probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH9 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486709 PE=4 SV=1)

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 655/727 (90.10%), Postives = 675/727 (92.85%), Query Frame = 0

Query: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLV 60
           MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSS EKQRQRLPVYKYRTAILYLV
Sbjct: 1   MAQFWKPGTEKPRLLDDGEGGVLFFSSSYSSSSGFGFSSTEKQRQRLPVYKYRTAILYLV 60

Query: 61  ETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120
           ETHATTIIVGETGSGKTTQIPQ+LKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK
Sbjct: 61  ETHATTIIVGETGSGKTTQIPQFLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVK 120

Query: 121 LGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180
           LGEEVGYTIRFEDLTN DVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM
Sbjct: 121 LGEEVGYTIRFEDLTNPDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDM 180

Query: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEG 240
           LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQ SKRR   EGE  EPKVEPAILSVEG
Sbjct: 181 LLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQMSKRRRALEGETFEPKVEPAILSVEG 240

Query: 241 RGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERN 300
           RGFNVQIFYLEEPVS+YVQS VSTVLSIHEQEPPGDILVFLTGQDDIDAA+QL+ EE +N
Sbjct: 241 RGFNVQIFYLEEPVSDYVQSAVSTVLSIHEQEPPGDILVFLTGQDDIDAAVQLIVEEGQN 300

Query: 301 DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 360
           DRK+SELI+LPLYSGLSRAEQ+L+FSPT RGKRKVVISTNIAETSLTLE           
Sbjct: 301 DRKRSELIILPLYSGLSRAEQDLIFSPTPRGKRKVVISTNIAETSLTLE----------- 360

Query: 361 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 420
                         GIVYV+DSGFSKQRFYNPI+DIESLI+APISKASARQRTGRAGRIR
Sbjct: 361 --------------GIVYVIDSGFSKQRFYNPITDIESLIVAPISKASARQRTGRAGRIR 420

Query: 421 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 480
           PGKCY RLYTEEYFIN+MPAEGIPEMQRSNLV+ VIQLKALGIDNILGFDWPSPPSPEVM
Sbjct: 421 PGKCY-RLYTEEYFINEMPAEGIPEMQRSNLVTCVIQLKALGIDNILGFDWPSPPSPEVM 480

Query: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 540
           VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEI+TIAA+LS
Sbjct: 481 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIMTIAAILS 540

Query: 541 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600
           I      QSIW SSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI
Sbjct: 541 I------QSIWASSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 600

Query: 601 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 660
           NYQAMKKV+EVREQLRRIAQRLGI+LKSCERDT A+RKAVTAGFFANA  LEAYSHNGMY
Sbjct: 601 NYQAMKKVMEVREQLRRIAQRLGIILKSCERDTTAIRKAVTAGFFANACQLEAYSHNGMY 660

Query: 661 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 720
           KT RGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNV+TIDPGWLTE APHFYQ R
Sbjct: 661 KTVRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVVTIDPGWLTEIAPHFYQQR 695

Query: 721 QLSHMPH 728
           QLSHMPH
Sbjct: 721 QLSHMPH 695

BLAST of MS013577.1 vs. TAIR 10
Match: AT4G18465.1 (RNA helicase family protein )

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 546/721 (75.73%), Postives = 627/721 (86.96%), Query Frame = 0

Query: 4   FWKPGTEKPRLLDDGEGGVLFFSSSY--SSSSGFGFSSIEKQRQRLPVYKYRTAILYLVE 63
           FWKPGTEKPR  +DGEGG++F S++   SSSS +G+++IEKQRQRLPVYKYRT ILYLVE
Sbjct: 3   FWKPGTEKPRFEEDGEGGIVFMSNNLASSSSSSYGYANIEKQRQRLPVYKYRTEILYLVE 62

Query: 64  THATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACTQPRRLAVQAVASRVAEEMGVKL 123
            HATTIIVGETGSGKTTQIPQYLKEAGWA+GGRVIACTQPRRLAVQAV++RVAEEMGV L
Sbjct: 63  NHATTIIVGETGSGKTTQIPQYLKEAGWAEGGRVIACTQPRRLAVQAVSARVAEEMGVNL 122

Query: 124 GEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPLLTKYSVIMVDEAHERSISTDML 183
           GEEVGYTIRFED T + VT VKFLTDGVL+REMM+DPLLTKYSVIM+DEAHERSISTD+L
Sbjct: 123 GEEVGYTIRFEDHTTSGVTSVKFLTDGVLIREMMEDPLLTKYSVIMIDEAHERSISTDIL 182

Query: 184 LGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRRHRFEGEALEPKVEPAILSVEGR 243
           LGLLKKIQRRRP+LRLIISSATIEAK+MS FF  SK+RH  EG    PK+EPAILSVEGR
Sbjct: 183 LGLLKKIQRRRPELRLIISSATIEAKTMSNFFNSSKKRHAPEGSTPGPKLEPAILSVEGR 242

Query: 244 GFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILVFLTGQDDIDAAIQLLREEERND 303
           GF+V+I Y+EEPVS+Y++S VST+L I+E+EPPGD+LVFLTGQ+DI+ AI+LL EE  ++
Sbjct: 243 GFSVKIHYVEEPVSDYIRSVVSTILLINEREPPGDVLVFLTGQEDIETAIKLLEEEAHSN 302

Query: 304 RKKSE-LIVLPLYSGLSRAEQELVFSPTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSY 363
           +K S  L+ LPLYSGLSR+EQEL+F+PT RGKRKV++STNIAETSLTLE           
Sbjct: 303 QKNSSGLLPLPLYSGLSRSEQELIFTPTPRGKRKVILSTNIAETSLTLE----------- 362

Query: 364 SLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIESLIIAPISKASARQRTGRAGRIR 423
                         G+VYV+DSGFSKQ+FYNPISDIESL++APISKASARQR+GRAGR+R
Sbjct: 363 --------------GVVYVIDSGFSKQKFYNPISDIESLVVAPISKASARQRSGRAGRVR 422

Query: 424 PGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVM 483
           PGKCY RLYTE+YF+N MP EGIPEMQRSNLVS+VIQLKALGIDNILGFDWP+PPS E M
Sbjct: 423 PGKCY-RLYTEDYFLNQMPGEGIPEMQRSNLVSTVIQLKALGIDNILGFDWPAPPSSEAM 482

Query: 484 VRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISKMILASGELGCSEEIITIAAVLS 543
           +RALEVLYSL +LDDDAKLTSP GFQVAE+PL+PMISKMILAS ELGCS EIITIAAVLS
Sbjct: 483 IRALEVLYSLQILDDDAKLTSPTGFQVAELPLDPMISKMILASSELGCSHEIITIAAVLS 542

Query: 544 IQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVTFLNVYKGFLQSNKSSQWCHKNFI 603
           +      QS+W  +RG QKE DEA+LRFAAAEGDHVTFLNVYKGFL+S K +QWC+KNF+
Sbjct: 543 V------QSVWIIARGVQKEQDEAKLRFAAAEGDHVTFLNVYKGFLESKKPTQWCYKNFL 602

Query: 604 NYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMY 663
           NYQ+MKKV+E+R+QL+RIA+RLGI LKSC+ D  AVRKAVTAGFFANA  LE +S NG+Y
Sbjct: 603 NYQSMKKVVEIRDQLKRIARRLGITLKSCDGDMEAVRKAVTAGFFANACRLEPHS-NGVY 662

Query: 664 KTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYR 722
           KT RGS+EVYIHPSSVLFRVNPKWV+Y S+VST+RQYMRNV+TI+P WLTE APHFYQ R
Sbjct: 663 KTIRGSEEVYIHPSSVLFRVNPKWVVYQSIVSTERQYMRNVVTINPSWLTEVAPHFYQNR 690

BLAST of MS013577.1 vs. TAIR 10
Match: AT1G32490.1 (RNA helicase family protein )

HSP 1 Score: 589.3 bits (1518), Expect = 4.1e-168
Identity = 318/685 (46.42%), Postives = 451/685 (65.84%), Query Frame = 0

Query: 40   IEKQRQRLPVYKYRTAILYLVETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACT 99
            +++ R+ LP+Y YR  +L  VE H   +IVG+TGSGKTTQIPQYL EAG+   G+V  CT
Sbjct: 397  LQEVRRSLPIYTYRDQLLKAVEEHQVLVIVGDTGSGKTTQIPQYLHEAGYTKRGKV-GCT 456

Query: 100  QPRRLAVQAVASRVAEEMGVKLGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPL 159
            QPRR+A  +VA+RVA+EMGVKLG EVGY+IRFED T +D T +K++TDG+LLRE++ +P 
Sbjct: 457  QPRRVAAMSVAARVAQEMGVKLGHEVGYSIRFEDCT-SDKTVLKYMTDGMLLRELLGEPD 516

Query: 160  LTKYSVIMVDEAHERSISTDMLLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRR 219
            L  YSV++VDEAHER++STD+L GL+K I R RPDL+L+ISSAT++A+  S +F  +   
Sbjct: 517  LASYSVVIVDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAEKFSDYFDTA--- 576

Query: 220  HRFEGEALEPKVEPAILSVEGRGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILV 279
                           I S  GR + V+I Y   P ++Y+ + + T+L+IH +EP GDILV
Sbjct: 577  --------------PIFSFPGRRYPVEINYTSAPEADYMDAAIVTILTIHVREPLGDILV 636

Query: 280  FLTGQDDIDAAIQLLREEERN-DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVIS 339
            F TGQ++I+ A ++L+   R    K  ELI+ P+Y+ L    Q  +F PT  G RKVV++
Sbjct: 637  FFTGQEEIETAEEILKHRIRGLGTKIRELIICPIYANLPSELQAKIFEPTPEGARKVVLA 696

Query: 340  TNIAETSLTLEVRFGFIKRLSYSLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIES 399
            TNIAETSLT++                         GI YVVD GFSK + YNP + +ES
Sbjct: 697  TNIAETSLTID-------------------------GIKYVVDPGFSKMKSYNPRTGMES 756

Query: 400  LIIAPISKASARQRTGRAGRIRPGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQL 459
            L+I PISKASA QR GRAGR  PGKCY RLYT   + ND+    +PE+QR+NL S V+ L
Sbjct: 757  LLITPISKASATQRAGRAGRTSPGKCY-RLYTAFNYNNDLEENTVPEVQRTNLASVVLAL 816

Query: 460  KALGIDNILGFDWPSPPSPEVMVRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISK 519
            K+LGI +++ FD+  PP  E +V++LE+L++LG L+   +LT   G ++AE PL+PM+SK
Sbjct: 817  KSLGIHDLINFDFMDPPPAEALVKSLELLFALGALNKLGELTK-AGRRMAEFPLDPMLSK 876

Query: 520  MILASGELGCSEEIITIAAVLSIQANVILQSIWTSSRGAQKELDEARLRFAAAE-GDHVT 579
            MI+ S +  CS+EII+IAA+LSI       SI+   +  Q   D AR+ F     GDH+ 
Sbjct: 877  MIVVSDKYKCSDEIISIAAMLSIGG-----SIFYRPKDKQVHADNARMNFHTGNVGDHIA 936

Query: 580  FLNVYKGFLQSNKSSQWCHKNFINYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVR 639
             L VY  + ++N S+QWC++N+I  ++MK+  ++R+QL  + +R+ I + S   +  +VR
Sbjct: 937  LLKVYSSWKETNFSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEIDISSNLNELDSVR 996

Query: 640  KAVTAGFFANASHLEAYSHNGMYKTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQY 699
            K++ AGFF + + L+    NG Y+T +  Q V+IHP+S L +V P+WV+YH LV T ++Y
Sbjct: 997  KSIVAGFFPHTAKLQ---KNGSYRTVKHPQTVHIHPNSGLSQVLPRWVVYHELVLTSKEY 1027

Query: 700  MRNVITIDPGWLTEAAPHFYQYRQL 723
            MR V  + P WL E APH+YQ + +
Sbjct: 1057 MRQVTELKPEWLIELAPHYYQLKDV 1027

BLAST of MS013577.1 vs. TAIR 10
Match: AT1G32490.2 (RNA helicase family protein )

HSP 1 Score: 589.3 bits (1518), Expect = 4.1e-168
Identity = 318/685 (46.42%), Postives = 451/685 (65.84%), Query Frame = 0

Query: 40   IEKQRQRLPVYKYRTAILYLVETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIACT 99
            +++ R+ LP+Y YR  +L  VE H   +IVG+TGSGKTTQIPQYL EAG+   G+V  CT
Sbjct: 387  LQEVRRSLPIYTYRDQLLKAVEEHQVLVIVGDTGSGKTTQIPQYLHEAGYTKRGKV-GCT 446

Query: 100  QPRRLAVQAVASRVAEEMGVKLGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDPL 159
            QPRR+A  +VA+RVA+EMGVKLG EVGY+IRFED T +D T +K++TDG+LLRE++ +P 
Sbjct: 447  QPRRVAAMSVAARVAQEMGVKLGHEVGYSIRFEDCT-SDKTVLKYMTDGMLLRELLGEPD 506

Query: 160  LTKYSVIMVDEAHERSISTDMLLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKRR 219
            L  YSV++VDEAHER++STD+L GL+K I R RPDL+L+ISSAT++A+  S +F  +   
Sbjct: 507  LASYSVVIVDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAEKFSDYFDTA--- 566

Query: 220  HRFEGEALEPKVEPAILSVEGRGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDILV 279
                           I S  GR + V+I Y   P ++Y+ + + T+L+IH +EP GDILV
Sbjct: 567  --------------PIFSFPGRRYPVEINYTSAPEADYMDAAIVTILTIHVREPLGDILV 626

Query: 280  FLTGQDDIDAAIQLLREEERN-DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVIS 339
            F TGQ++I+ A ++L+   R    K  ELI+ P+Y+ L    Q  +F PT  G RKVV++
Sbjct: 627  FFTGQEEIETAEEILKHRIRGLGTKIRELIICPIYANLPSELQAKIFEPTPEGARKVVLA 686

Query: 340  TNIAETSLTLEVRFGFIKRLSYSLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIES 399
            TNIAETSLT++                         GI YVVD GFSK + YNP + +ES
Sbjct: 687  TNIAETSLTID-------------------------GIKYVVDPGFSKMKSYNPRTGMES 746

Query: 400  LIIAPISKASARQRTGRAGRIRPGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQL 459
            L+I PISKASA QR GRAGR  PGKCY RLYT   + ND+    +PE+QR+NL S V+ L
Sbjct: 747  LLITPISKASATQRAGRAGRTSPGKCY-RLYTAFNYNNDLEENTVPEVQRTNLASVVLAL 806

Query: 460  KALGIDNILGFDWPSPPSPEVMVRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMISK 519
            K+LGI +++ FD+  PP  E +V++LE+L++LG L+   +LT   G ++AE PL+PM+SK
Sbjct: 807  KSLGIHDLINFDFMDPPPAEALVKSLELLFALGALNKLGELTK-AGRRMAEFPLDPMLSK 866

Query: 520  MILASGELGCSEEIITIAAVLSIQANVILQSIWTSSRGAQKELDEARLRFAAAE-GDHVT 579
            MI+ S +  CS+EII+IAA+LSI       SI+   +  Q   D AR+ F     GDH+ 
Sbjct: 867  MIVVSDKYKCSDEIISIAAMLSIGG-----SIFYRPKDKQVHADNARMNFHTGNVGDHIA 926

Query: 580  FLNVYKGFLQSNKSSQWCHKNFINYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVR 639
             L VY  + ++N S+QWC++N+I  ++MK+  ++R+QL  + +R+ I + S   +  +VR
Sbjct: 927  LLKVYSSWKETNFSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEIDISSNLNELDSVR 986

Query: 640  KAVTAGFFANASHLEAYSHNGMYKTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQY 699
            K++ AGFF + + L+    NG Y+T +  Q V+IHP+S L +V P+WV+YH LV T ++Y
Sbjct: 987  KSIVAGFFPHTAKLQ---KNGSYRTVKHPQTVHIHPNSGLSQVLPRWVVYHELVLTSKEY 1017

Query: 700  MRNVITIDPGWLTEAAPHFYQYRQL 723
            MR V  + P WL E APH+YQ + +
Sbjct: 1047 MRQVTELKPEWLIELAPHYYQLKDV 1017

BLAST of MS013577.1 vs. TAIR 10
Match: AT3G26560.1 (ATP-dependent RNA helicase, putative )

HSP 1 Score: 589.0 bits (1517), Expect = 5.4e-168
Identity = 317/681 (46.55%), Postives = 442/681 (64.90%), Query Frame = 0

Query: 39   SIEKQRQRLPVYKYRTAILYLVETHATTIIVGETGSGKTTQIPQYLKEAGWADGGRVIAC 98
            SI++QR+ LP+YK +  ++  V  +   +++GETGSGKTTQ+ QYL EAG+   G+ I C
Sbjct: 507  SIQEQRESLPIYKLKKELIQAVHDNQVLVVIGETGSGKTTQVTQYLAEAGYTTKGK-IGC 566

Query: 99   TQPRRLAVQAVASRVAEEMGVKLGEEVGYTIRFEDLTNTDVTRVKFLTDGVLLREMMDDP 158
            TQPRR+A  +VA RVAEE G +LGEEVGY IRFED T  D T +K++TDG+LLRE++ D 
Sbjct: 567  TQPRRVAAMSVAKRVAEEFGCRLGEEVGYAIRFEDCTGPD-TVIKYMTDGMLLREILIDE 626

Query: 159  LLTKYSVIMVDEAHERSISTDMLLGLLKKIQRRRPDLRLIISSATIEAKSMSTFFQPSKR 218
             L++YSVIM+DEAHER+I TD+L GLLKK+ +RR DLRLI++SAT++A+  S +F     
Sbjct: 627  NLSQYSVIMLDEAHERTIHTDVLFGLLKKLMKRRLDLRLIVTSATLDAEKFSGYF----- 686

Query: 219  RHRFEGEALEPKVEPAILSVEGRGFNVQIFYLEEPVSNYVQSTVSTVLSIHEQEPPGDIL 278
                            I ++ GR F V+I Y ++P ++Y+ + + TVL IH  EP GDIL
Sbjct: 687  ------------FNCNIFTIPGRTFPVEILYTKQPETDYLDAALITVLQIHLTEPEGDIL 746

Query: 279  VFLTGQDDIDAAIQLLREEERN-DRKKSELIVLPLYSGLSRAEQELVFSPTTRGKRKVVI 338
            VFLTGQ++ID+A Q L E  +   +   ELI+LP+YS L    Q  +F P   GKRKVV+
Sbjct: 747  VFLTGQEEIDSACQSLYERMKGLGKNVPELIILPVYSALPSEMQSRIFDPPPPGKRKVVV 806

Query: 339  STNIAETSLTLEVRFGFIKRLSYSLAIPHSGPFYSVQGIVYVVDSGFSKQRFYNPISDIE 398
            +TNIAE SLT++                         GI YVVD GF+KQ  YNP   +E
Sbjct: 807  ATNIAEASLTID-------------------------GIYYVVDPGFAKQNVYNPKQGLE 866

Query: 399  SLIIAPISKASARQRTGRAGRIRPGKCYSRLYTEEYFINDMPAEGIPEMQRSNLVSSVIQ 458
            SL+I PIS+ASA+QR GRAGR  PGKCY RLYTE  + N+MP   IPE+QR NL  + + 
Sbjct: 867  SLVITPISQASAKQRAGRAGRTGPGKCY-RLYTESAYRNEMPPTSIPEIQRINLGMTTLT 926

Query: 459  LKALGIDNILGFDWPSPPSPEVMVRALEVLYSLGVLDDDAKLTSPVGFQVAEIPLEPMIS 518
            +KA+GI+++L FD+  PP P+ ++ A+E LYSLG LD++  LT  +G ++AE PLEP +S
Sbjct: 927  MKAMGINDLLSFDFMDPPQPQALISAMEQLYSLGALDEEGLLTK-LGRKMAEFPLEPPLS 986

Query: 519  KMILASGELGCSEEIITIAAVLSIQANVILQSIWTSSRGAQKELDEARLRFAAAEGDHVT 578
            KM+LAS +LGCS+EI+T+ A++         +I+   R  Q + D+ R +F   EGDH+T
Sbjct: 987  KMLLASVDLGCSDEILTMIAMIQ------TGNIFYRPREKQAQADQKRAKFFQPEGDHLT 1046

Query: 579  FLNVYKGFLQSNKSSQWCHKNFINYQAMKKVIEVREQLRRIAQRLGIVLKSCERDTMAVR 638
             L VY+ +   N S  WC +NFI  +++++  +VR+QL  I  +  + + +  ++   +R
Sbjct: 1047 LLAVYEAWKAKNFSGPWCFENFIQSRSLRRAQDVRKQLLSIMDKYKLDVVTAGKNFTKIR 1106

Query: 639  KAVTAGFFANASHLEAYSHNGMYKTTRGSQEVYIHPSSVLFRVNPKWVIYHSLVSTDRQY 698
            KA+TAGFF + +  +       Y+T   +Q VYIHPSS LF+  P WVIYH LV T ++Y
Sbjct: 1107 KAITAGFFFHGARKDPQEG---YRTLVENQPVYIHPSSALFQRQPDWVIYHDLVMTTKEY 1132

Query: 699  MRNVITIDPGWLTEAAPHFYQ 719
            MR V  IDP WL E AP F++
Sbjct: 1167 MREVTVIDPKWLVELAPRFFK 1132

BLAST of MS013577.1 vs. TAIR 10
Match: AT2G35340.1 (helicase domain-containing protein )

HSP 1 Score: 584.7 bits (1506), Expect = 1.0e-166
Identity = 318/697 (45.62%), Postives = 452/697 (64.85%), Query Frame = 0

Query: 28   SYSSSSGFGFSSIEKQRQRLPVYKYRTAILYLVETHATTIIVGETGSGKTTQIPQYLKEA 87
            S  S+    F  +++ R+ LP+Y YR  +L  V+ H   IIVGETGSGKTTQIPQYL EA
Sbjct: 385  SQDSAGKSAFHMLQEDRKALPIYTYRDQLLNAVKDHQVLIIVGETGSGKTTQIPQYLHEA 444

Query: 88   GWADGGRVIACTQPRRLAVQAVASRVAEEMGVKLGEEVGYTIRFEDLTNTDVTRVKFLTD 147
            G+   G+V  CTQPRR+A  +VA+RVA+EMG KLG EVGY+IRFED T ++ T +K++TD
Sbjct: 445  GYTKLGKV-GCTQPRRVAAMSVAARVAQEMGGKLGHEVGYSIRFEDCT-SEKTILKYMTD 504

Query: 148  GVLLREMMDDPLLTKYSVIMVDEAHERSISTDMLLGLLKKIQRRRPDLRLIISSATIEAK 207
            G+LLRE++ +P L  YSVI+VDEAHER++ TD+L GL+K I R RPDL+L+ISSAT++A+
Sbjct: 505  GMLLRELLGEPDLGSYSVIIVDEAHERTLRTDILFGLVKDIARARPDLKLLISSATMDAE 564

Query: 208  SMSTFFQPSKRRHRFEGEALEPKVEPAILSVEGRGFNVQIFYLEEPVSNYVQSTVSTVLS 267
              S FF                  +  I    GR + V I +   P ++Y+ + ++TVL+
Sbjct: 565  KFSDFFD-----------------QAPIFRFPGRRYPVDICFTTAPEADYMDAAITTVLT 624

Query: 268  IHEQEPPGDILVFLTGQDDIDAAIQLLREEERN-DRKKSELIVLPLYSGLSRAEQELVFS 327
            IH +EP GD+LVFL GQ++I+A  + L+ + R    K  ELI+ P+Y+ L    Q  +F 
Sbjct: 625  IHVKEPLGDVLVFLPGQEEIEAVEENLKHKIRGLGTKIRELIICPIYANLPSELQAKIFE 684

Query: 328  PTTRGKRKVVISTNIAETSLTLEVRFGFIKRLSYSLAIPHSGPFYSVQGIVYVVDSGFSK 387
            PT  G RKVV++TNIAETSLT++                         GI YVVD GFSK
Sbjct: 685  PTPEGARKVVLATNIAETSLTID-------------------------GIKYVVDPGFSK 744

Query: 388  QRFYNPISDIESLIIAPISKASARQRTGRAGRIRPGKCYSRLYTEEYFINDMPAEGIPEM 447
             + YNP + +ESL++ PISKASA QRTGRAGR  PGKCY RLYT   + ND+    +PE+
Sbjct: 745  MKSYNPRTGMESLLVTPISKASATQRTGRAGRTSPGKCY-RLYTAFNYYNDLEDNTVPEI 804

Query: 448  QRSNLVSSVIQLKALGIDNILGFDWPSPPSPEVMVRALEVLYSLGVLDDDAKLTSPVGFQ 507
            QR+NL S V+ LK+LGI N+L FD+  PP  E ++++LE+L++LG L+   +LT   G +
Sbjct: 805  QRTNLASVVLSLKSLGIHNLLNFDFMDPPPSEALIKSLELLFALGALNQLGELTK-AGRR 864

Query: 508  VAEIPLEPMISKMILASGELGCSEEIITIAAVLSIQANVILQSIWTSSRGAQKELDEARL 567
            +AE PL+PM+SKMI+ S +  CS+EII+IAA+LSI       SI+   +  Q   D A  
Sbjct: 865  MAEFPLDPMLSKMIVVSDKYKCSDEIISIAAMLSIG-----PSIFYRPKDKQVHADNAMK 924

Query: 568  RFAAAE-GDHVTFLNVYKGFLQSNKSSQWCHKNFINYQAMKKVIEVREQLRRIAQRLGIV 627
             F     GDH+ FL +Y  + ++N S+QWC++N+I  ++MK+  ++R+QL  + +R+ I 
Sbjct: 925  NFHVGNVGDHIAFLKIYNSWKETNYSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEID 984

Query: 628  LKSCERDTMAVRKAVTAGFFANASHLEAYSHNGMYKTTRGSQEVYIHPSSVLFRVNPKWV 687
            + S   +  ++RK++ AGFF + + L+    NG Y+T +  Q V+IHP+S L +V P+WV
Sbjct: 985  VSSNANELDSIRKSIVAGFFPHTAKLQ---KNGSYRTVKHPQTVHIHPASGLSQVLPRWV 1027

Query: 688  IYHSLVSTDRQYMRNVITIDPGWLTEAAPHFYQYRQL 723
            +YH LV T ++YMR V  + P WL E APH+YQ + +
Sbjct: 1045 VYHQLVLTSKEYMRQVTELKPEWLIEIAPHYYQLKDV 1027