MS012863.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS012863.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	aspartic proteinase CDR1-like
Location	scaffold63: 4085090 .. 4086400 (-)
Sequence length	1311
RNA-Seq Expression	MS012863.1
Synteny	MS012863.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCACTTCACTCTTCTTCATTCTCCTTCTCTTCTCCTTCTCCGAAGCAACTACTCGTAGCCGCGGTGGCGGCTTCACCACCTCTCTCTTTCACCGAGATTCTTCTCTCTCTCCTCTCCACGACCCATCTCTCTCCCCTTACGATCGCCTCAACAACGCCGTCCGCCGCTCCTTCTCCCGCTCTGCCACCATCTCCCACCGCGCCGCCGCCGCCGCCGCCTCCACCGCCGCCGTCACCTCTCCAATCCTCCCCGCCAGCGGCGAGTTTCTAATGGACGTCTCCCTCGGAACCCCGCCGGTGGATTTCTTCGGCATCGCCGATACCGGAAGCGACCTGACGTGGACCCAGTGCCTGCCATGTCGGCAATGCTTCAACCAATCACGCCCCATTTTTAATCCACGTCGGTCCTCCTCCTACCGCCGCGTGTCATGCGCGTCCGATACTTGCTCCTCCCTCGAATCCGCCGGCTGTGGGCCCGACGGCCGAACCTGCGGCTACAGCTACTCCTACGGAGATAGGTCCTACACGCGCGGAGAGCTAGGGCTTGACAAAATTACCATCGGGTCCTTCCAGCTCCGCGACACCGTGATCGGATGCGGCCACGAGAACGGCGGCACTTTCGGCAGCGGAGCTTCGTCGGGGATTATCGGACTCGGCGGCGGCGGTCTCTCTTTGGTCTCTCAGTTGAACGAAATCGCCGCCGTCAGCCGGCAGTTCTCTTATTGCTTGCCCAATGTCTTCAGCAACGCGAATCTCACCGGCACAATAAACTTCGGGCGGGACGCCGTCATTTCGGGCCGTAATGCCGTTTCGACGCCGCTCGTCCCGAAAAATCCGGACACGTACTATTTCCTGACTCTCGAAGCGATCTCCGTGGGAAACACGCGCCACGCGGCCGACATGTCGTCCGCGGCGAATGGAGGAATAATGGGGAACATAATCATCGATTCCGGGACGACACTAACGTTACTTCCTCAGGACTTATACGACGGCGTCGTTTCGACTTTGGGCAGACTCATCCGGGCGAAGCGGGCGGAAGATCCGGCCGGGCTGCTGGAACTATGCTACGCCACAAACGAGATCGGCGACGGAGATATTCCGATCATCACCGCCCATTTCGGCGGCGGTGCGGCCGTGAAGCTGCTGCCGTTGAACACGTTTGGGGAGGTCGCTGAAAACGTGAGTTGCTTGACATTTGCGCCGTCGTCGGGGTTGGCCATTTTTGGGAACTTGGCTCAGATGAACTTTTTGGTCGGATATGATCTTGAGCAGAGGAGGCTGTCATTTAAGCCCACCGTCTGTTCT

mRNA sequence

ATGGCTGCCACTTCACTCTTCTTCATTCTCCTTCTCTTCTCCTTCTCCGAAGCAACTACTCGTAGCCGCGGTGGCGGCTTCACCACCTCTCTCTTTCACCGAGATTCTTCTCTCTCTCCTCTCCACGACCCATCTCTCTCCCCTTACGATCGCCTCAACAACGCCGTCCGCCGCTCCTTCTCCCGCTCTGCCACCATCTCCCACCGCGCCGCCGCCGCCGCCGCCTCCACCGCCGCCGTCACCTCTCCAATCCTCCCCGCCAGCGGCGAGTTTCTAATGGACGTCTCCCTCGGAACCCCGCCGGTGGATTTCTTCGGCATCGCCGATACCGGAAGCGACCTGACGTGGACCCAGTGCCTGCCATGTCGGCAATGCTTCAACCAATCACGCCCCATTTTTAATCCACGTCGGTCCTCCTCCTACCGCCGCGTGTCATGCGCGTCCGATACTTGCTCCTCCCTCGAATCCGCCGGCTGTGGGCCCGACGGCCGAACCTGCGGCTACAGCTACTCCTACGGAGATAGGTCCTACACGCGCGGAGAGCTAGGGCTTGACAAAATTACCATCGGGTCCTTCCAGCTCCGCGACACCGTGATCGGATGCGGCCACGAGAACGGCGGCACTTTCGGCAGCGGAGCTTCGTCGGGGATTATCGGACTCGGCGGCGGCGGTCTCTCTTTGGTCTCTCAGTTGAACGAAATCGCCGCCGTCAGCCGGCAGTTCTCTTATTGCTTGCCCAATGTCTTCAGCAACGCGAATCTCACCGGCACAATAAACTTCGGGCGGGACGCCGTCATTTCGGGCCGTAATGCCGTTTCGACGCCGCTCGTCCCGAAAAATCCGGACACGTACTATTTCCTGACTCTCGAAGCGATCTCCGTGGGAAACACGCGCCACGCGGCCGACATGTCGTCCGCGGCGAATGGAGGAATAATGGGGAACATAATCATCGATTCCGGGACGACACTAACGTTACTTCCTCAGGACTTATACGACGGCGTCGTTTCGACTTTGGGCAGACTCATCCGGGCGAAGCGGGCGGAAGATCCGGCCGGGCTGCTGGAACTATGCTACGCCACAAACGAGATCGGCGACGGAGATATTCCGATCATCACCGCCCATTTCGGCGGCGGTGCGGCCGTGAAGCTGCTGCCGTTGAACACGTTTGGGGAGGTCGCTGAAAACGTGAGTTGCTTGACATTTGCGCCGTCGTCGGGGTTGGCCATTTTTGGGAACTTGGCTCAGATGAACTTTTTGGTCGGATATGATCTTGAGCAGAGGAGGCTGTCATTTAAGCCCACCGTCTGTTCT

Coding sequence (CDS)

ATGGCTGCCACTTCACTCTTCTTCATTCTCCTTCTCTTCTCCTTCTCCGAAGCAACTACTCGTAGCCGCGGTGGCGGCTTCACCACCTCTCTCTTTCACCGAGATTCTTCTCTCTCTCCTCTCCACGACCCATCTCTCTCCCCTTACGATCGCCTCAACAACGCCGTCCGCCGCTCCTTCTCCCGCTCTGCCACCATCTCCCACCGCGCCGCCGCCGCCGCCGCCTCCACCGCCGCCGTCACCTCTCCAATCCTCCCCGCCAGCGGCGAGTTTCTAATGGACGTCTCCCTCGGAACCCCGCCGGTGGATTTCTTCGGCATCGCCGATACCGGAAGCGACCTGACGTGGACCCAGTGCCTGCCATGTCGGCAATGCTTCAACCAATCACGCCCCATTTTTAATCCACGTCGGTCCTCCTCCTACCGCCGCGTGTCATGCGCGTCCGATACTTGCTCCTCCCTCGAATCCGCCGGCTGTGGGCCCGACGGCCGAACCTGCGGCTACAGCTACTCCTACGGAGATAGGTCCTACACGCGCGGAGAGCTAGGGCTTGACAAAATTACCATCGGGTCCTTCCAGCTCCGCGACACCGTGATCGGATGCGGCCACGAGAACGGCGGCACTTTCGGCAGCGGAGCTTCGTCGGGGATTATCGGACTCGGCGGCGGCGGTCTCTCTTTGGTCTCTCAGTTGAACGAAATCGCCGCCGTCAGCCGGCAGTTCTCTTATTGCTTGCCCAATGTCTTCAGCAACGCGAATCTCACCGGCACAATAAACTTCGGGCGGGACGCCGTCATTTCGGGCCGTAATGCCGTTTCGACGCCGCTCGTCCCGAAAAATCCGGACACGTACTATTTCCTGACTCTCGAAGCGATCTCCGTGGGAAACACGCGCCACGCGGCCGACATGTCGTCCGCGGCGAATGGAGGAATAATGGGGAACATAATCATCGATTCCGGGACGACACTAACGTTACTTCCTCAGGACTTATACGACGGCGTCGTTTCGACTTTGGGCAGACTCATCCGGGCGAAGCGGGCGGAAGATCCGGCCGGGCTGCTGGAACTATGCTACGCCACAAACGAGATCGGCGACGGAGATATTCCGATCATCACCGCCCATTTCGGCGGCGGTGCGGCCGTGAAGCTGCTGCCGTTGAACACGTTTGGGGAGGTCGCTGAAAACGTGAGTTGCTTGACATTTGCGCCGTCGTCGGGGTTGGCCATTTTTGGGAACTTGGCTCAGATGAACTTTTTGGTCGGATATGATCTTGAGCAGAGGAGGCTGTCATTTAAGCCCACCGTCTGTTCT

Protein sequence

MAATSLFFILLLFSFSEATTRSRGGGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTRGELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRHAADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNFLVGYDLEQRRLSFKPTVCS

Homology

[Close]

BLAST of MS012863.1 vs. NCBI nr
Match: XP_022136655.1 (aspartic proteinase CDR1-like [Momordica charantia])

HSP 1 Score: 693.0 bits (1787), Expect = 1.7e-195
Identity = 358/437 (81.92%), Postives = 385/437 (88.10%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRGGGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSF 60
           MAATS+FFILL FSFSEATT S   GFT SLFHRDS        SLSPYDRLNNAVRRSF
Sbjct: 1   MAATSVFFILLFFSFSEATTHST-NGFTASLFHRDS--------SLSPYDRLNNAVRRSF 60

Query: 61  SRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCL 120
           SRSAT+  RAAA  ++TAA   PI+PA+GEFLM+VSLGTPPV F GIADTGSDLTWTQCL
Sbjct: 61  SRSATLFRRAAAGVSTTAA-KFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCL 120

Query: 121 PCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTRG 180
           PC QCFNQS PIFNPR SS+YR+VSC S TC SL+++GCGPDGRTCGY Y+YGD+SYT G
Sbjct: 121 PCNQCFNQSGPIFNPRGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYG 180

Query: 181 ELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQ 240
           ELG DKITIGSF+LR+TVIGCGHENGGTFGSGASSGIIGLGGG LSL SQLN+I AV R+
Sbjct: 181 ELGRDKITIGSFRLRNTVIGCGHENGGTFGSGASSGIIGLGGGSLSLASQLNKIGAVRRR 240

Query: 241 FSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRHA 300
           FSYCLPN+F NANLTGTINFG  AV+SGR AVSTPLVPKNPDTYY+LTLEA+SVGNTRHA
Sbjct: 241 FSYCLPNIFRNANLTGTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHA 300

Query: 301 ADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYAT 360
           ADMSSA  GG MGNIIIDSGTTLT LPQ+LYDGVVSTL ++IRAKRAEDPAG+LELCYAT
Sbjct: 301 ADMSSAVEGG-MGNIIIDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYAT 360

Query: 361 NEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNFLV 420
           NEI DG IP ITAHFGG AAVKLLPLNTFGEVAENVSCLTFAPSS LAIFGNLAQMNFLV
Sbjct: 361 NEIRDGHIPTITAHFGGSAAVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLV 420

Query: 421 GYDLEQRRLSFKPTVCS 438
           GYDL  RRLSFKPTVC+
Sbjct: 421 GYDLHARRLSFKPTVCA 426

[Close]

BLAST of MS012863.1 vs. NCBI nr
Match: XP_038889220.1 (aspartic proteinase CDR1-like [Benincasa hispida])

HSP 1 Score: 581.3 bits (1497), Expect = 7.2e-162
Identity = 307/439 (69.93%), Postives = 353/439 (80.41%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATT-RSRGGGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRS 60
           MAA S+FF  LLFSF+EATT R  G GFTTSLFHRDS LSPLH+ SLS +DR  NA RRS
Sbjct: 1   MAAISIFFYFLLFSFAEATTNRGGGNGFTTSLFHRDSLLSPLHNSSLSCHDRRTNAFRRS 60

Query: 61  FSRSAT-ISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQ 120
           FSRSAT +SH     A STA + SPI+P SGEFLM VS+GTPPVDF  IADTGSDLTWTQ
Sbjct: 61  FSRSATLLSH---VNAVSTACIHSPIIPNSGEFLMSVSIGTPPVDFIAIADTGSDLTWTQ 120

Query: 121 CLPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYT 180
           CLPC++CFNQS P+FNPRRSSSYR VSC SDTC SL+S  CG D +TC Y YSYGDRS+T
Sbjct: 121 CLPCQKCFNQSHPMFNPRRSSSYRNVSCTSDTCRSLDSYHCGTDLQTCSYGYSYGDRSFT 180

Query: 181 RGELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVS 240
            G+L  DKITI SF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ+N IAA+ 
Sbjct: 181 YGDLASDKITIESFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGALSLVSQMNTIAAIK 240

Query: 241 RQFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTR 300
           RQFSYCLP  FS+ N+TG I+FG++AV+SG   +STPLV ++PDT+YFLTLEAISV N R
Sbjct: 241 RQFSYCLPTFFSDENITGKISFGQNAVVSGPKVISTPLVSRSPDTFYFLTLEAISVANKR 300

Query: 301 -HAADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELC 360
             AAD +SA      GNIIIDSGTTLT LP++LY+ +VSTL  +I+AKR +DP+G+LELC
Sbjct: 301 LKAADDTSALTR--RGNIIIDSGTTLTFLPRNLYEDLVSTLVSVIKAKRVDDPSGILELC 360

Query: 361 YATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMN 420
           YA     D  IP+I AHF GGA VKLLPLNTF  VAENV+CLT AP+S LAIFGNLAQ+N
Sbjct: 361 YAAGGGDDLHIPVIIAHFAGGADVKLLPLNTFALVAENVTCLTLAPASDLAIFGNLAQIN 420

Query: 421 FLVGYDLEQRRLSFKPTVC 437
           F+VGYDLE +RLSFKPTVC
Sbjct: 421 FIVGYDLENKRLSFKPTVC 433

[Close]

BLAST of MS012863.1 vs. NCBI nr
Match: KAA0044968.1 (putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 577.0 bits (1486), Expect = 1.4e-160
Identity = 300/440 (68.18%), Postives = 351/440 (79.77%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRG-GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRS 60
           M   S+FF  LLF  S+AT    G  GFTTSLFHRDS LSPLH+PSLS YD L  + RRS
Sbjct: 1   MPVISIFFYFLLFFSSKATAHGGGHHGFTTSLFHRDSLLSPLHNPSLSRYDSLVESFRRS 60

Query: 61  FSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQC 120
           FSRSAT+ +     + STA + SPI+P SGEFLM + +GTP V+F  IADTGSDLTWTQC
Sbjct: 61  FSRSATLLNH--LTSVSTACIRSPIIPDSGEFLMSIFIGTPRVNFIAIADTGSDLTWTQC 120

Query: 121 LPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTR 180
           LPCR+CFNQS+PIFNPRRSSSYR+VSC+SDTC SLES+ CG D ++C Y YSYGDRS+T 
Sbjct: 121 LPCRECFNQSQPIFNPRRSSSYRKVSCSSDTCRSLESSHCGLDLKSCSYGYSYGDRSFTY 180

Query: 181 GELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSR 240
           G+L  DKITIGSF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ++ IA V  
Sbjct: 181 GDLASDKITIGSFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGSLSLVSQMSTIAGVKP 240

Query: 241 QFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRH 300
           QFSYCLP  FSN N+TG I+FGR AV+SGR  VSTPLVP++PDT+YFLTLEAISVGN R 
Sbjct: 241 QFSYCLPTFFSNENITGKISFGRKAVVSGRQVVSTPLVPRSPDTFYFLTLEAISVGNKRF 300

Query: 301 --AADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELC 360
             A DMS+  N    GNIIIDSGTTLTLLP+ LYDGVVSTL R+I+AKR +DP+G+LELC
Sbjct: 301 KAAKDMSAMTN---QGNIIIDSGTTLTLLPRSLYDGVVSTLARVIKAKRVDDPSGILELC 360

Query: 361 YATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMN 420
           Y+  ++ D +IPIITAHF G A VKLLP+NTF  VA+NV CLT AP++ +AIFGNLAQ+N
Sbjct: 361 YSAGQLEDLNIPIITAHFSGRADVKLLPVNTFAPVADNVICLTLAPATNVAIFGNLAQIN 420

Query: 421 FLVGYDLEQRRLSFKPTVCS 438
           F VGYDL  +RLSFKPT C+
Sbjct: 421 FEVGYDLGNKRLSFKPTRCA 434

[Close]

BLAST of MS012863.1 vs. NCBI nr
Match: XP_008452150.1 (PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16499.1 putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 575.9 bits (1483), Expect = 3.0e-160
Identity = 299/440 (67.95%), Postives = 351/440 (79.77%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRG-GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRS 60
           M A S+FF  LLF  S+AT    G  GFTTSL+HRDS LSPLH+PSLS YD L  + RRS
Sbjct: 1   MPAISIFFYFLLFFSSKATAHGGGHHGFTTSLYHRDSLLSPLHNPSLSRYDSLVESFRRS 60

Query: 61  FSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQC 120
           FSRSAT+ +     + STA + SPI+P SGEFLM + +GTP V+F  IADTGSDLTWTQC
Sbjct: 61  FSRSATLLNH--LTSVSTACIRSPIIPDSGEFLMSIFIGTPRVNFIAIADTGSDLTWTQC 120

Query: 121 LPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTR 180
           LPCR+CFNQS+PIFNPRRSSSYR+VSC+SDTC SLES+ CG D ++C Y YSYGDRS+T 
Sbjct: 121 LPCRECFNQSQPIFNPRRSSSYRKVSCSSDTCRSLESSHCGLDLKSCSYGYSYGDRSFTY 180

Query: 181 GELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSR 240
           G+L  DKITIGSF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ++ IA V  
Sbjct: 181 GDLASDKITIGSFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGSLSLVSQMSTIAGVKP 240

Query: 241 QFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRH 300
           QFSYCLP  FSN N+TG I+FGR AV+SGR  VSTPLVP++PDT+YFLTLEAISVGN R 
Sbjct: 241 QFSYCLPTFFSNENITGKISFGRKAVVSGRQVVSTPLVPRSPDTFYFLTLEAISVGNKRF 300

Query: 301 --AADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELC 360
             A DMS+  N    GNIIIDSGTTLTLLP+ LYDGVVSTL R+I+ KR +DP+G+LELC
Sbjct: 301 KAAKDMSAMTN---QGNIIIDSGTTLTLLPRSLYDGVVSTLARVIKTKRVDDPSGILELC 360

Query: 361 YATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMN 420
           Y+  ++ D +IPIITAHF G A VKLLP+NTF  VA+NV CLT AP++ +AIFGNLAQ+N
Sbjct: 361 YSAGQLEDLNIPIITAHFSGRADVKLLPVNTFAPVADNVICLTLAPATNVAIFGNLAQIN 420

Query: 421 FLVGYDLEQRRLSFKPTVCS 438
           F VGYDL  +RLSFKPT C+
Sbjct: 421 FEVGYDLGNKRLSFKPTRCA 434

[Close]

BLAST of MS012863.1 vs. NCBI nr
Match: XP_004149004.1 (probable aspartic protease At2g35615 [Cucumis sativus] >KGN53446.1 hypothetical protein Csa_015341 [Cucumis sativus])

HSP 1 Score: 572.4 bits (1474), Expect = 3.3e-159
Identity = 299/439 (68.11%), Postives = 350/439 (79.73%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRGG--GFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRR 60
           MAA S+FF  LLF FS   T   GG  GFTTSLF RDS LSPLH+PSLS YD L +A RR
Sbjct: 1   MAAISIFFYFLLF-FSSKVTAHGGGHHGFTTSLFRRDSPLSPLHNPSLSRYDSLIDAFRR 60

Query: 61  SFSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQ 120
           SFSRSAT+       + STA + SPI+P SGEFLM + +GTPPV+   IADTGSDLTWTQ
Sbjct: 61  SFSRSATL--LTHLTSVSTACIRSPIIPDSGEFLMSIFIGTPPVNVIAIADTGSDLTWTQ 120

Query: 121 CLPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYT 180
           CLPCR+CFNQS+PIFNPRRSSSYR+VSCASDTC SLES  CGPD ++C Y YSYGDRS+T
Sbjct: 121 CLPCRECFNQSQPIFNPRRSSSYRKVSCASDTCRSLESYHCGPDLQSCSYGYSYGDRSFT 180

Query: 181 RGELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVS 240
            G+L  D+ITIGSF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ+  IA V 
Sbjct: 181 YGDLASDQITIGSFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGSLSLVSQMRTIAGVK 240

Query: 241 RQFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTR 300
            +FSYCLP  FSNAN+TGTI+FGR AV+SGR  VSTPLVP++PDT+YFLTLEAISVG  R
Sbjct: 241 PRFSYCLPTFFSNANITGTISFGRKAVVSGRQVVSTPLVPRSPDTFYFLTLEAISVGKKR 300

Query: 301 HAADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCY 360
             A    +A     GNIIIDSGTTLTLLP+ LY GV STL R+I+AKR +DP+G+LELCY
Sbjct: 301 FKAANGISAMTN-HGNIIIDSGTTLTLLPRSLYYGVFSTLARVIKAKRVDDPSGILELCY 360

Query: 361 ATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNF 420
           +  ++ D +IPIITAHF GGA VKLLP+NTF  VA+NV+CLTFAP++ +AIFGNLAQ+NF
Sbjct: 361 SAGQVDDLNIPIITAHFAGGADVKLLPVNTFAPVADNVTCLTFAPATQVAIFGNLAQINF 420

Query: 421 LVGYDLEQRRLSFKPTVCS 438
            VGYDL  +RLSF+P +C+
Sbjct: 421 EVGYDLGNKRLSFEPKLCA 434

[Close]

BLAST of MS012863.1 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 3.5e-95
Identity = 192/419 (45.82%), Postives = 265/419 (63.25%), Query Frame = 0


Query: 26  GFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSRSATISHRAAAAAASTAAVTSPIL 85
           GFT  L HRDS  SP ++P  +   RL NA+ RS +R    + +      +T      + 
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEK-----DNTPQPQIDLT 89

Query: 86  PASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCRQCFNQSRPIFNPRRSSSYRRVS 145
             SGE+LM+VS+GTPP     IADTGSDL WTQC PC  C+ Q  P+F+P+ SS+Y+ VS
Sbjct: 90  SNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVS 149

Query: 146 CASDTCSSLES-AGCGPDGRTCGYSYSYGDRSYTRGELGLDKITIGS-----FQLRDTVI 205
           C+S  C++LE+ A C  +  TC YS SYGD SYT+G + +D +T+GS      QL++ +I
Sbjct: 150 CSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIII 209

Query: 206 GCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQFSYCLPNVFSNANLTGTIN 265
           GCGH N GTF     SGI+GLGGG +SL+ QL +  ++  +FSYCL  + S  + T  IN
Sbjct: 210 GCGHNNAGTFNK-KGSGIVGLGGGPVSLIKQLGD--SIDGKFSYCLVPLTSKKDQTSKIN 269

Query: 266 FGRDAVISGRNAVSTPLVPK-NPDTYYFLTLEAISVGNTRHAADMSSAANGGIMGNIIID 325
           FG +A++SG   VSTPL+ K + +T+Y+LTL++ISVG+ +     S + +    GNIIID
Sbjct: 270 FGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQ--IQYSGSDSESSEGNIIID 329

Query: 326 SGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYATNEIGDGDIPIITAHFGGG 385
           SGTTLTLLP + Y  +   +   I A++ +DP   L LCY+    GD  +P+IT HF  G
Sbjct: 330 SGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSAT--GDLKVPVITMHF-DG 389

Query: 386 AAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNFLVGYDLEQRRLSFKPTVCS 438
           A VKL   N F +V+E++ C  F  S   +I+GN+AQMNFLVGYD   + +SFKPT C+
Sbjct: 390 ADVKLDSSNAFVQVSEDLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of MS012863.1 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.3e-92
Identity = 200/449 (44.54%), Postives = 274/449 (61.02%), Query Frame = 0


Query: 9   ILLLFSFSEATTRSRG--GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSRSATI 68
           +L  F F   T  S G    F+  L HRDS LSP+++P ++  DRLN A  RS SRS   
Sbjct: 6   LLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRF 65

Query: 69  SHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCRQCF 128
           +H+      S   + S ++ A GEF M +++GTPP+  F IADTGSDLTW QC PC+QC+
Sbjct: 66  NHQ-----LSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCY 125

Query: 129 NQSRPIFNPRRSSSYRRVSCASDTCSSLESA--GCGPDGRTCGYSYSYGDRSYTRGELGL 188
            ++ PIF+ ++SS+Y+   C S  C +L S   GC      C Y YSYGD+S+++G++  
Sbjct: 126 KENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVAT 185

Query: 189 DKITIGS-----FQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSR 248
           + ++I S          TV GCG+ NGGTF     SGIIGLGGG LSL+SQL   +++S+
Sbjct: 186 ETVSIDSASGSPVSFPGTVFGCGYNNGGTFDE-TGSGIIGLGGGHLSLISQLG--SSISK 245

Query: 249 QFSYCLPNVFSNANLTGTINFGRDAVISG----RNAVSTPLVPKNPDTYYFLTLEAISVG 308
           +FSYCL +  +  N T  IN G +++ S        VSTPLV K P TYY+LTLEAISVG
Sbjct: 246 KFSYCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVG 305

Query: 309 NTR--HAADMSSAANGGIM----GNIIIDSGTTLTLLPQDLYDGVVSTLGRLIR-AKRAE 368
             +  +     +  + GI+    GNIIIDSGTTLTLL    +D   S +   +  AKR  
Sbjct: 306 KKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVS 365

Query: 369 DPAGLLELCYATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLA 428
           DP GLL  C+ +     G +P IT HF  GA V+L P+N F +++E++ CL+  P++ +A
Sbjct: 366 DPQGLLSHCFKSGSAEIG-LPEITVHF-TGADVRLSPINAFVKLSEDMVCLSMVPTTEVA 425

Query: 429 IFGNLAQMNFLVGYDLEQRRLSFKPTVCS 438
           I+GN AQM+FLVGYDLE R +SF+   CS
Sbjct: 426 IYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

[Close]

BLAST of MS012863.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.6e-58
Identity = 147/396 (37.12%), Postives = 206/396 (52.02%), Query Frame = 0


Query: 45  SLSPYDRLNNAVRRSFSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDF 104
           +L+ Y+ +  A++R   R  +I+    A   S++ + +P+    GE+LM+V++GTP   F
Sbjct: 54  NLTKYELIKRAIKRGERRMRSIN----AMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSF 113

Query: 105 FGIADTGSDLTWTQCLPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGR 164
             I DTGSDL WTQC PC QCF+Q  PIFNP+ SSS+  + C S  C  L S  C  +  
Sbjct: 114 SAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETC--NNN 173

Query: 165 TCGYSYSYGDRSYTRGELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGG 224
            C Y+Y YGD S T+G +  +  T  +  + +   GCG +N G FG G  +G+IG+G G 
Sbjct: 174 ECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQG-FGQGNGAGLIGMGWGP 233

Query: 225 LSLVSQLNEIAAVSRQFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTY 284
           LSL SQL        QFSYC+ +  S++  T  +      V  G  + +      NP TY
Sbjct: 234 LSLPSQLG-----VGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNP-TY 293

Query: 285 YFLTLEAISVGNTRHAADMSS-AANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIR 344
           Y++TL+ I+VG        S+        G +IIDSGTTLT LPQD Y+ V       I 
Sbjct: 294 YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN 353

Query: 345 AKRAEDPAGLLELCYATNEIGDG-DIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFA 404
               ++ +  L  C+     G    +P I+  F GG  + L   N     AE V CL   
Sbjct: 354 LPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILISPAEGVICLAMG 413

Query: 405 PSS--GLAIFGNLAQMNFLVGYDLEQRRLSFKPTVC 437
            SS  G++IFGN+ Q    V YDL+   +SF PT C
Sbjct: 414 SSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of MS012863.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.5e-57
Identity = 159/448 (35.49%), Postives = 228/448 (50.89%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATT------RSRGGGFTTSLFHRDSSLSPLHDPSLSPYDRLNN 60
           + A S+ +I +  + S + T       ++  GF   L H DS        +L+ +  L  
Sbjct: 9   LLALSIVYIFVAPTHSTSRTALNHRHEAKVTGFQIMLEHVDSG------KNLTKFQLLER 68

Query: 61  AVRRSFSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDL 120
           A+ R   R      R  A     + V + +    GE+LM++S+GTP   F  I DTGSDL
Sbjct: 69  AIERGSRR----LQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDL 128

Query: 121 TWTQCLPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGD 180
            WTQC PC QCFNQS PIFNP+ SSS+  + C+S  C +L S  C      C Y+Y YGD
Sbjct: 129 IWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTC--SNNFCQYTYGYGD 188

Query: 181 RSYTRGELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEI 240
            S T+G +G + +T GS  + +   GCG  N G FG G  +G++G+G G LSL SQL+  
Sbjct: 189 GSETQGSMGTETLTFGSVSIPNITFGCGENNQG-FGQGNGAGLVGMGRGPLSLPSQLD-- 248

Query: 241 AAVSRQFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNP-DTYYFLTLEAIS 300
                +FSYC+  + S+      +    ++V +G  + +T L+  +   T+Y++TL  +S
Sbjct: 249 ---VTKFSYCMTPIGSSTPSNLLLGSLANSVTAG--SPNTTLIQSSQIPTFYYITLNGLS 308

Query: 301 VGNTRHAADMSSAA---NGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDP 360
           VG+TR   D S+ A   N G  G IIIDSGTTLT    + Y  V       I        
Sbjct: 309 VGSTRLPIDPSAFALNSNNG-TGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGS 368

Query: 361 AGLLELCYAT-NEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSS-GLA 420
           +   +LC+ T ++  +  IP    HF GG  ++L   N F   +  + CL    SS G++
Sbjct: 369 SSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFISPSNGLICLAMGSSSQGMS 428

Query: 421 IFGNLAQMNFLVGYDLEQRRLSFKPTVC 437
           IFGN+ Q N LV YD     +SF    C
Sbjct: 429 IFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of MS012863.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.0e-51
Identity = 148/444 (33.33%), Postives = 223/444 (50.23%), Query Frame = 0


Query: 10  LLLFSFSEATTRSRGGGFTTSLFHRDSSLSPLHDPSL--SPYDRLNNAVRRSFSRSATIS 69
           LL   F   +        T +L H D+  S      L  S   R +  V+   + +A I 
Sbjct: 55  LLESEFESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIP 114

Query: 70  HR----AAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCR 129
            R    A      +++V S +   SGE+   + +GTP    + + DTGSD+ W QC PCR
Sbjct: 115 GRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCR 174

Query: 130 QCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTRGELG 189
           +C++QS PIF+PR+S +Y  + C+S  C  L+SAGC    +TC Y  SYGD S+T G+  
Sbjct: 175 RCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFS 234

Query: 190 LDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQFSY 249
            + +T    +++   +GCGH+N G F   A  G++GLG G LS   Q       +++FSY
Sbjct: 235 TETLTFRRNRVKGVALGCGHDNEGLFVGAA--GLLGLGKGKLSFPGQTGH--RFNQKFSY 294

Query: 250 CLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNP--DTYYFLTLEAISVGNTRHAA 309
           CL +  S ++   ++ FG  AV   R A  TPL+  NP  DT+Y++ L  ISVG TR   
Sbjct: 295 CLVD-RSASSKPSSVVFGNAAV--SRIARFTPLL-SNPKLDTFYYVGLLGISVGGTRVPG 354

Query: 310 DMSSAANGGIMGN--IIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYA 369
             +S      +GN  +IIDSGT++T L +  Y  +        +  +      L + C+ 
Sbjct: 355 VTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFD 414

Query: 370 TNEIGDGDIPIITAHFGGG-----AAVKLLPLNTFGEVAENVSCLTFAPS-SGLAIFGNL 429
            + + +  +P +  HF G      A   L+P++T G+      C  FA +  GL+I GN+
Sbjct: 415 LSNMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKF-----CFAFAGTMGGLSIIGNI 474

Query: 430 AQMNFLVGYDLEQRRLSFKPTVCS 438
            Q  F V YDL   R+ F P  C+
Sbjct: 475 QQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of MS012863.1 vs. ExPASy TrEMBL
Match: A0A6J1C4J6 (aspartic proteinase CDR1-like OS=Momordica charantia OX=3673 GN=LOC111008307 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 8.2e-196
Identity = 358/437 (81.92%), Postives = 385/437 (88.10%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRGGGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSF 60
           MAATS+FFILL FSFSEATT S   GFT SLFHRDS        SLSPYDRLNNAVRRSF
Sbjct: 1   MAATSVFFILLFFSFSEATTHST-NGFTASLFHRDS--------SLSPYDRLNNAVRRSF 60

Query: 61  SRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCL 120
           SRSAT+  RAAA  ++TAA   PI+PA+GEFLM+VSLGTPPV F GIADTGSDLTWTQCL
Sbjct: 61  SRSATLFRRAAAGVSTTAA-KFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCL 120

Query: 121 PCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTRG 180
           PC QCFNQS PIFNPR SS+YR+VSC S TC SL+++GCGPDGRTCGY Y+YGD+SYT G
Sbjct: 121 PCNQCFNQSGPIFNPRGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYG 180

Query: 181 ELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQ 240
           ELG DKITIGSF+LR+TVIGCGHENGGTFGSGASSGIIGLGGG LSL SQLN+I AV R+
Sbjct: 181 ELGRDKITIGSFRLRNTVIGCGHENGGTFGSGASSGIIGLGGGSLSLASQLNKIGAVRRR 240

Query: 241 FSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRHA 300
           FSYCLPN+F NANLTGTINFG  AV+SGR AVSTPLVPKNPDTYY+LTLEA+SVGNTRHA
Sbjct: 241 FSYCLPNIFRNANLTGTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHA 300

Query: 301 ADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYAT 360
           ADMSSA  GG MGNIIIDSGTTLT LPQ+LYDGVVSTL ++IRAKRAEDPAG+LELCYAT
Sbjct: 301 ADMSSAVEGG-MGNIIIDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYAT 360

Query: 361 NEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNFLV 420
           NEI DG IP ITAHFGG AAVKLLPLNTFGEVAENVSCLTFAPSS LAIFGNLAQMNFLV
Sbjct: 361 NEIRDGHIPTITAHFGGSAAVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLV 420

Query: 421 GYDLEQRRLSFKPTVCS 438
           GYDL  RRLSFKPTVC+
Sbjct: 421 GYDLHARRLSFKPTVCA 426

[Close]

BLAST of MS012863.1 vs. ExPASy TrEMBL
Match: A0A5A7TPZ5 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002740 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 6.6e-161
Identity = 300/440 (68.18%), Postives = 351/440 (79.77%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRG-GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRS 60
           M   S+FF  LLF  S+AT    G  GFTTSLFHRDS LSPLH+PSLS YD L  + RRS
Sbjct: 1   MPVISIFFYFLLFFSSKATAHGGGHHGFTTSLFHRDSLLSPLHNPSLSRYDSLVESFRRS 60

Query: 61  FSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQC 120
           FSRSAT+ +     + STA + SPI+P SGEFLM + +GTP V+F  IADTGSDLTWTQC
Sbjct: 61  FSRSATLLNH--LTSVSTACIRSPIIPDSGEFLMSIFIGTPRVNFIAIADTGSDLTWTQC 120

Query: 121 LPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTR 180
           LPCR+CFNQS+PIFNPRRSSSYR+VSC+SDTC SLES+ CG D ++C Y YSYGDRS+T 
Sbjct: 121 LPCRECFNQSQPIFNPRRSSSYRKVSCSSDTCRSLESSHCGLDLKSCSYGYSYGDRSFTY 180

Query: 181 GELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSR 240
           G+L  DKITIGSF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ++ IA V  
Sbjct: 181 GDLASDKITIGSFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGSLSLVSQMSTIAGVKP 240

Query: 241 QFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRH 300
           QFSYCLP  FSN N+TG I+FGR AV+SGR  VSTPLVP++PDT+YFLTLEAISVGN R 
Sbjct: 241 QFSYCLPTFFSNENITGKISFGRKAVVSGRQVVSTPLVPRSPDTFYFLTLEAISVGNKRF 300

Query: 301 --AADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELC 360
             A DMS+  N    GNIIIDSGTTLTLLP+ LYDGVVSTL R+I+AKR +DP+G+LELC
Sbjct: 301 KAAKDMSAMTN---QGNIIIDSGTTLTLLPRSLYDGVVSTLARVIKAKRVDDPSGILELC 360

Query: 361 YATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMN 420
           Y+  ++ D +IPIITAHF G A VKLLP+NTF  VA+NV CLT AP++ +AIFGNLAQ+N
Sbjct: 361 YSAGQLEDLNIPIITAHFSGRADVKLLPVNTFAPVADNVICLTLAPATNVAIFGNLAQIN 420

Query: 421 FLVGYDLEQRRLSFKPTVCS 438
           F VGYDL  +RLSFKPT C+
Sbjct: 421 FEVGYDLGNKRLSFKPTRCA 434

[Close]

BLAST of MS012863.1 vs. ExPASy TrEMBL
Match: A0A5D3D1Z7 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003070 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.5e-160
Identity = 299/440 (67.95%), Postives = 351/440 (79.77%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRG-GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRS 60
           M A S+FF  LLF  S+AT    G  GFTTSL+HRDS LSPLH+PSLS YD L  + RRS
Sbjct: 1   MPAISIFFYFLLFFSSKATAHGGGHHGFTTSLYHRDSLLSPLHNPSLSRYDSLVESFRRS 60

Query: 61  FSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQC 120
           FSRSAT+ +     + STA + SPI+P SGEFLM + +GTP V+F  IADTGSDLTWTQC
Sbjct: 61  FSRSATLLNH--LTSVSTACIRSPIIPDSGEFLMSIFIGTPRVNFIAIADTGSDLTWTQC 120

Query: 121 LPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTR 180
           LPCR+CFNQS+PIFNPRRSSSYR+VSC+SDTC SLES+ CG D ++C Y YSYGDRS+T 
Sbjct: 121 LPCRECFNQSQPIFNPRRSSSYRKVSCSSDTCRSLESSHCGLDLKSCSYGYSYGDRSFTY 180

Query: 181 GELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSR 240
           G+L  DKITIGSF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ++ IA V  
Sbjct: 181 GDLASDKITIGSFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGSLSLVSQMSTIAGVKP 240

Query: 241 QFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRH 300
           QFSYCLP  FSN N+TG I+FGR AV+SGR  VSTPLVP++PDT+YFLTLEAISVGN R 
Sbjct: 241 QFSYCLPTFFSNENITGKISFGRKAVVSGRQVVSTPLVPRSPDTFYFLTLEAISVGNKRF 300

Query: 301 --AADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELC 360
             A DMS+  N    GNIIIDSGTTLTLLP+ LYDGVVSTL R+I+ KR +DP+G+LELC
Sbjct: 301 KAAKDMSAMTN---QGNIIIDSGTTLTLLPRSLYDGVVSTLARVIKTKRVDDPSGILELC 360

Query: 361 YATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMN 420
           Y+  ++ D +IPIITAHF G A VKLLP+NTF  VA+NV CLT AP++ +AIFGNLAQ+N
Sbjct: 361 YSAGQLEDLNIPIITAHFSGRADVKLLPVNTFAPVADNVICLTLAPATNVAIFGNLAQIN 420

Query: 421 FLVGYDLEQRRLSFKPTVCS 438
           F VGYDL  +RLSFKPT C+
Sbjct: 421 FEVGYDLGNKRLSFKPTRCA 434

[Close]

BLAST of MS012863.1 vs. ExPASy TrEMBL
Match: A0A1S3BT75 (probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493255 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.5e-160
Identity = 299/440 (67.95%), Postives = 351/440 (79.77%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRG-GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRS 60
           M A S+FF  LLF  S+AT    G  GFTTSL+HRDS LSPLH+PSLS YD L  + RRS
Sbjct: 1   MPAISIFFYFLLFFSSKATAHGGGHHGFTTSLYHRDSLLSPLHNPSLSRYDSLVESFRRS 60

Query: 61  FSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQC 120
           FSRSAT+ +     + STA + SPI+P SGEFLM + +GTP V+F  IADTGSDLTWTQC
Sbjct: 61  FSRSATLLNH--LTSVSTACIRSPIIPDSGEFLMSIFIGTPRVNFIAIADTGSDLTWTQC 120

Query: 121 LPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTR 180
           LPCR+CFNQS+PIFNPRRSSSYR+VSC+SDTC SLES+ CG D ++C Y YSYGDRS+T 
Sbjct: 121 LPCRECFNQSQPIFNPRRSSSYRKVSCSSDTCRSLESSHCGLDLKSCSYGYSYGDRSFTY 180

Query: 181 GELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSR 240
           G+L  DKITIGSF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ++ IA V  
Sbjct: 181 GDLASDKITIGSFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGSLSLVSQMSTIAGVKP 240

Query: 241 QFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRH 300
           QFSYCLP  FSN N+TG I+FGR AV+SGR  VSTPLVP++PDT+YFLTLEAISVGN R 
Sbjct: 241 QFSYCLPTFFSNENITGKISFGRKAVVSGRQVVSTPLVPRSPDTFYFLTLEAISVGNKRF 300

Query: 301 --AADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELC 360
             A DMS+  N    GNIIIDSGTTLTLLP+ LYDGVVSTL R+I+ KR +DP+G+LELC
Sbjct: 301 KAAKDMSAMTN---QGNIIIDSGTTLTLLPRSLYDGVVSTLARVIKTKRVDDPSGILELC 360

Query: 361 YATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMN 420
           Y+  ++ D +IPIITAHF G A VKLLP+NTF  VA+NV CLT AP++ +AIFGNLAQ+N
Sbjct: 361 YSAGQLEDLNIPIITAHFSGRADVKLLPVNTFAPVADNVICLTLAPATNVAIFGNLAQIN 420

Query: 421 FLVGYDLEQRRLSFKPTVCS 438
           F VGYDL  +RLSFKPT C+
Sbjct: 421 FEVGYDLGNKRLSFKPTRCA 434

[Close]

BLAST of MS012863.1 vs. ExPASy TrEMBL
Match: A0A0A0KZZ3 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G055410 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.6e-159
Identity = 299/439 (68.11%), Postives = 350/439 (79.73%), Query Frame = 0


Query: 1   MAATSLFFILLLFSFSEATTRSRGG--GFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRR 60
           MAA S+FF  LLF FS   T   GG  GFTTSLF RDS LSPLH+PSLS YD L +A RR
Sbjct: 1   MAAISIFFYFLLF-FSSKVTAHGGGHHGFTTSLFRRDSPLSPLHNPSLSRYDSLIDAFRR 60

Query: 61  SFSRSATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQ 120
           SFSRSAT+       + STA + SPI+P SGEFLM + +GTPPV+   IADTGSDLTWTQ
Sbjct: 61  SFSRSATL--LTHLTSVSTACIRSPIIPDSGEFLMSIFIGTPPVNVIAIADTGSDLTWTQ 120

Query: 121 CLPCRQCFNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYT 180
           CLPCR+CFNQS+PIFNPRRSSSYR+VSCASDTC SLES  CGPD ++C Y YSYGDRS+T
Sbjct: 121 CLPCRECFNQSQPIFNPRRSSSYRKVSCASDTCRSLESYHCGPDLQSCSYGYSYGDRSFT 180

Query: 181 RGELGLDKITIGSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVS 240
            G+L  D+ITIGSF+L  TVIGCGH+NGGTFG G +SGIIGLGGG LSLVSQ+  IA V 
Sbjct: 181 YGDLASDQITIGSFKLPKTVIGCGHQNGGTFG-GVTSGIIGLGGGSLSLVSQMRTIAGVK 240

Query: 241 RQFSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTR 300
            +FSYCLP  FSNAN+TGTI+FGR AV+SGR  VSTPLVP++PDT+YFLTLEAISVG  R
Sbjct: 241 PRFSYCLPTFFSNANITGTISFGRKAVVSGRQVVSTPLVPRSPDTFYFLTLEAISVGKKR 300

Query: 301 HAADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCY 360
             A    +A     GNIIIDSGTTLTLLP+ LY GV STL R+I+AKR +DP+G+LELCY
Sbjct: 301 FKAANGISAMTN-HGNIIIDSGTTLTLLPRSLYYGVFSTLARVIKAKRVDDPSGILELCY 360

Query: 361 ATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNF 420
           +  ++ D +IPIITAHF GGA VKLLP+NTF  VA+NV+CLTFAP++ +AIFGNLAQ+NF
Sbjct: 361 SAGQVDDLNIPIITAHFAGGADVKLLPVNTFAPVADNVTCLTFAPATQVAIFGNLAQINF 420

Query: 421 LVGYDLEQRRLSFKPTVCS 438
            VGYDL  +RLSF+P +C+
Sbjct: 421 EVGYDLGNKRLSFEPKLCA 434

[Close]

BLAST of MS012863.1 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.5e-104
Identity = 208/417 (49.88%), Postives = 270/417 (64.75%), Query Frame = 0


Query: 26  GFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSRSATISHRAAAAAASTAAVTSPIL 85
           GFT  L HRDS  SP ++ + +   R+ NA+RRS   +   S+  A+  +  + +TS   
Sbjct: 25  GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARSTLQFSNDDASPNSPQSFITS--- 84

Query: 86  PASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCRQCFNQSRPIFNPRRSSSYRRVS 145
              GE+LM++S+GTPPV    IADTGSDL WTQC PC  C+ Q+ P+F+P+ SS+YR+VS
Sbjct: 85  -NRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCYQQTSPLFDPKESSTYRKVS 144

Query: 146 CASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTRGELGLDKITIGS-----FQLRDTVIG 205
           C+S  C +LE A C  D  TC Y+ +YGD SYT+G++ +D +T+GS       LR+ +IG
Sbjct: 145 CSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMGSSGRRPVSLRNMIIG 204

Query: 206 CGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQFSYCLPNVFSNANLTGTINF 265
           CGHEN GTF   A SGIIGLGGG  SLVSQL +  +++ +FSYCL    S   LT  INF
Sbjct: 205 CGHENTGTF-DPAGSGIIGLGGGSTSLVSQLRK--SINGKFSYCLVPFTSETGLTSKINF 264

Query: 266 GRDAVISGRNAVSTPLVPKNPDTYYFLTLEAISVGNTRHAADMSSAANGGIMGNIIIDSG 325
           G + ++SG   VST +V K+P TYYFL LEAISVG+ +     +S   G   GNI+IDSG
Sbjct: 265 GTNGIVSGDGVVSTSMVKKDPATYYFLNLEAISVGSKK--IQFTSTIFGTGEGNIVIDSG 324

Query: 326 TTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYATNEIGDGDIPIITAHFGGGAA 385
           TTLTLLP + Y  + S +   I+A+R +DP G+L LCY   +     +P IT HF GG  
Sbjct: 325 TTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCY--RDSSSFKVPDITVHFKGG-D 384

Query: 386 VKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNFLVGYDLEQRRLSFKPTVCS 438
           VKL  LNTF  V+E+VSC  FA +  L IFGNLAQMNFLVGYD     +SFK T CS
Sbjct: 385 VKLGNLNTFVAVSEDVSCFAFAANEQLTIFGNLAQMNFLVGYDTVSGTVSFKKTDCS 429

[Close]

BLAST of MS012863.1 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 350.1 bits (897), Expect = 2.5e-96
Identity = 192/419 (45.82%), Postives = 265/419 (63.25%), Query Frame = 0


Query: 26  GFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSRSATISHRAAAAAASTAAVTSPIL 85
           GFT  L HRDS  SP ++P  +   RL NA+ RS +R    + +      +T      + 
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEK-----DNTPQPQIDLT 89

Query: 86  PASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCRQCFNQSRPIFNPRRSSSYRRVS 145
             SGE+LM+VS+GTPP     IADTGSDL WTQC PC  C+ Q  P+F+P+ SS+Y+ VS
Sbjct: 90  SNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVS 149

Query: 146 CASDTCSSLES-AGCGPDGRTCGYSYSYGDRSYTRGELGLDKITIGS-----FQLRDTVI 205
           C+S  C++LE+ A C  +  TC YS SYGD SYT+G + +D +T+GS      QL++ +I
Sbjct: 150 CSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIII 209

Query: 206 GCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQFSYCLPNVFSNANLTGTIN 265
           GCGH N GTF     SGI+GLGGG +SL+ QL +  ++  +FSYCL  + S  + T  IN
Sbjct: 210 GCGHNNAGTFNK-KGSGIVGLGGGPVSLIKQLGD--SIDGKFSYCLVPLTSKKDQTSKIN 269

Query: 266 FGRDAVISGRNAVSTPLVPK-NPDTYYFLTLEAISVGNTRHAADMSSAANGGIMGNIIID 325
           FG +A++SG   VSTPL+ K + +T+Y+LTL++ISVG+ +     S + +    GNIIID
Sbjct: 270 FGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQ--IQYSGSDSESSEGNIIID 329

Query: 326 SGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYATNEIGDGDIPIITAHFGGG 385
           SGTTLTLLP + Y  +   +   I A++ +DP   L LCY+    GD  +P+IT HF  G
Sbjct: 330 SGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSAT--GDLKVPVITMHF-DG 389

Query: 386 AAVKLLPLNTFGEVAENVSCLTFAPSSGLAIFGNLAQMNFLVGYDLEQRRLSFKPTVCS 438
           A VKL   N F +V+E++ C  F  S   +I+GN+AQMNFLVGYD   + +SFKPT C+
Sbjct: 390 ADVKLDSSNAFVQVSEDLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of MS012863.1 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 341.7 bits (875), Expect = 9.0e-94
Identity = 200/449 (44.54%), Postives = 274/449 (61.02%), Query Frame = 0


Query: 9   ILLLFSFSEATTRSRG--GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSRSATI 68
           +L  F F   T  S G    F+  L HRDS LSP+++P ++  DRLN A  RS SRS   
Sbjct: 6   LLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRF 65

Query: 69  SHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCRQCF 128
           +H+      S   + S ++ A GEF M +++GTPP+  F IADTGSDLTW QC PC+QC+
Sbjct: 66  NHQ-----LSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCY 125

Query: 129 NQSRPIFNPRRSSSYRRVSCASDTCSSLESA--GCGPDGRTCGYSYSYGDRSYTRGELGL 188
            ++ PIF+ ++SS+Y+   C S  C +L S   GC      C Y YSYGD+S+++G++  
Sbjct: 126 KENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVAT 185

Query: 189 DKITIGS-----FQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSR 248
           + ++I S          TV GCG+ NGGTF     SGIIGLGGG LSL+SQL   +++S+
Sbjct: 186 ETVSIDSASGSPVSFPGTVFGCGYNNGGTFDE-TGSGIIGLGGGHLSLISQLG--SSISK 245

Query: 249 QFSYCLPNVFSNANLTGTINFGRDAVISG----RNAVSTPLVPKNPDTYYFLTLEAISVG 308
           +FSYCL +  +  N T  IN G +++ S        VSTPLV K P TYY+LTLEAISVG
Sbjct: 246 KFSYCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVG 305

Query: 309 NTR--HAADMSSAANGGIM----GNIIIDSGTTLTLLPQDLYDGVVSTLGRLIR-AKRAE 368
             +  +     +  + GI+    GNIIIDSGTTLTLL    +D   S +   +  AKR  
Sbjct: 306 KKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVS 365

Query: 369 DPAGLLELCYATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSGLA 428
           DP GLL  C+ +     G +P IT HF  GA V+L P+N F +++E++ CL+  P++ +A
Sbjct: 366 DPQGLLSHCFKSGSAEIG-LPEITVHF-TGADVRLSPINAFVKLSEDMVCLSMVPTTEVA 425

Query: 429 IFGNLAQMNFLVGYDLEQRRLSFKPTVCS 438
           I+GN AQM+FLVGYDLE R +SF+   CS
Sbjct: 426 IYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

[Close]

BLAST of MS012863.1 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-88
Identity = 196/451 (43.46%), Postives = 266/451 (58.98%), Query Frame = 0


Query: 4   TSLFFILLLFSFSEATTRSRG-GGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSR 63
           T L+  LL  SF  A+  S      T  L HRDS  SPL++P  +  DRLN A  RS SR
Sbjct: 5   TFLYCSLLAISFFFASNSSANRENLTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISR 64

Query: 64  SATISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPC 123
           S   +        +   + S ++   GE+ M +S+GTPP   F IADTGSDLTW QC PC
Sbjct: 65  SRRFT--------TKTDLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPC 124

Query: 124 RQCFNQSRPIFNPRRSSSYRRVSCASDTCSSL--ESAGCGPDGRTCGYSYSYGDRSYTRG 183
           +QC+ Q+ P+F+ ++SS+Y+  SC S TC +L     GC      C Y YSYGD S+T+G
Sbjct: 125 QQCYKQNSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKG 184

Query: 184 ELGLDKITI-----GSFQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIA 243
           ++  + I+I      S     TV GCG+ NGGTF     SGIIGLGGG LSLVSQL   +
Sbjct: 185 DVATETISIDSSSGSSVSFPGTVFGCGYNNGGTF-EETGSGIIGLGGGPLSLVSQLG--S 244

Query: 244 AVSRQFSYCLPNVFSNANLTGTINFGRDAVISGRN----AVSTPLVPKNPDTYYFLTLEA 303
           ++ ++FSYCL +  +  N T  IN G +++ S  +     ++TPL+ K+P+TYYFLTLEA
Sbjct: 245 SIGKKFSYCLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEA 304

Query: 304 ISVGNTRHAADMSSAANGG----IMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIR-AKR 363
           ++VG T+           G      GNIIIDSGTTLTLL    YD   + +   +  AKR
Sbjct: 305 VTVGKTKLPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKR 364

Query: 364 AEDPAGLLELCYATNEIGDGDIPIITAHFGGGAAVKLLPLNTFGEVAENVSCLTFAPSSG 423
             DP GLL  C+ + +   G +P IT HF   A VKL P+N F ++ E+  CL+  P++ 
Sbjct: 365 VSDPQGLLTHCFKSGDKEIG-LPAITMHF-TNADVKLSPINAFVKLNEDTVCLSMIPTTE 424

Query: 424 LAIFGNLAQMNFLVGYDLEQRRLSFKPTVCS 438
           +AI+GN+ QM+FLVGYDLE + +SF+   CS
Sbjct: 425 VAIYGNMVQMDFLVGYDLETKTVSFQRMDCS 442

[Close]

BLAST of MS012863.1 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 219.5 bits (558), Expect = 5.1e-57
Identity = 158/442 (35.75%), Postives = 219/442 (49.55%), Query Frame = 0


Query: 6   LFFILLLFSFSEATTRSRGGGFTTSLFHRDSSLSPLHDPSLSPYDRLNNAVRRSFSRSAT 65
           + F+ +   F   TT S   GFT  L HR S+ S           R++N    S   + T
Sbjct: 9   VLFLQISLCFLFTTTASPPHGFTMDLIHRRSNAS----------SRVSNTQSGSSPYANT 68

Query: 66  ISHRAAAAAASTAAVTSPILPASGEFLMDVSLGTPPVDFFGIADTGSDLTWTQCLPCRQC 125
           +   +                    +LM + +GTPP +   I DTGS++TWTQCLPC  C
Sbjct: 69  VFDNSV-------------------YLMKLQVGTPPFEIQAIIDTGSEITWTQCLPCVHC 128

Query: 126 FNQSRPIFNPRRSSSYRRVSCASDTCSSLESAGCGPDGRTCGYSYSYGDRSYTRGELGLD 185
           + Q+ PIF+P +SS+++   C               DG +C Y   Y D +YT G L  +
Sbjct: 129 YEQNAPIFDPSKSSTFKEKRC---------------DGHSCPYEVDYFDHTYTMGTLATE 188

Query: 186 KITIGS-----FQLRDTVIGCGHENGGTFGSGASSGIIGLGGGGLSLVSQLNEIAAVSRQ 245
            IT+ S     F + +T+IGCGH N  ++   + SG++GL  G  SL++Q+         
Sbjct: 189 TITLHSTSGEPFVMPETIIGCGHNN--SWFKPSFSGMVGLNWGPSSLITQMG--GEYPGL 248

Query: 246 FSYCLPNVFSNANLTGTINFGRDAVISGRNAVSTPL-VPKNPDTYYFLTLEAISVGNTRH 305
            SYC    FS    T  INFG +A+++G   VST + +      +Y+L L+A+SVGNTR 
Sbjct: 249 MSYC----FSGQG-TSKINFGANAIVAGDGVVSTTMFMTTAKPGFYYLNLDAVSVGNTR- 308

Query: 306 AADMSSAANGGIMGNIIIDSGTTLTLLPQDLYDGVVSTLGRLIRAKRAEDPAGLLELCYA 365
             +        + GNI+IDSGTTLT  P    + V   +  ++ A RA DP G   LCY 
Sbjct: 309 -IETMGTTFHALEGNIVIDSGTTLTYFPVSYCNLVRQAVEHVVTAVRAADPTGNDMLCYN 368

Query: 366 TNEIGDGDI-PIITAHFGGGAAVKLLPLNTFGEVAE-NVSCLTFAPSSGL--AIFGNLAQ 425
           ++ I   DI P+IT HF GG  + L   N + E     V CL    +S    AIFGN AQ
Sbjct: 369 SDTI---DIFPVITMHFSGGVDLVLDKYNMYMESNNGGVFCLAIICNSPTQEAIFGNRAQ 392

Query: 426 MNFLVGYDLEQRRLSFKPTVCS 438
            NFLVGYD     +SF PT CS
Sbjct: 429 NNFLVGYDSSSLLVSFSPTNCS 392

The following BLAST results are available for this feature:

BLAST of MS012863.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136655.1	1.7e-195	81.92	aspartic proteinase CDR1-like [Momordica charantia]	[more]
XP_038889220.1	7.2e-162	69.93	aspartic proteinase CDR1-like [Benincasa hispida]	[more]
KAA0044968.1	1.4e-160	68.18	putative aspartic protease [Cucumis melo var. makuwa]	[more]
XP_008452150.1	3.0e-160	67.95	PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16499.1 putat...	[more]
XP_004149004.1	3.3e-159	68.11	probable aspartic protease At2g35615 [Cucumis sativus] >KGN53446.1 hypothetical ...	[more]

BLAST of MS012863.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6XBF8	3.5e-95	45.82	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q3EBM5	1.3e-92	44.54	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q766C2	1.6e-58	37.12	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	6.5e-57	35.49	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LNJ3	7.0e-51	33.33	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of MS012863.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4J6	8.2e-196	81.92	aspartic proteinase CDR1-like OS=Momordica charantia OX=3673 GN=LOC111008307 PE=...	[more]
A0A5A7TPZ5	6.6e-161	68.18	Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A5D3D1Z7	1.5e-160	67.95	Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3BT75	1.5e-160	67.95	probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493255 PE=...	[more]
A0A0A0KZZ3	1.6e-159	68.11	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G05541...	[more]

BLAST of MS012863.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64830.1	1.5e-104	49.88	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	2.5e-96	45.82	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	9.0e-94	44.54	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	1.5e-88	43.46	Eukaryotic aspartyl protease family protein	[more]
AT2G28010.1	5.1e-57	35.75	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 91..261 e-value: 1.4E-50 score: 172.0
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 285..432 e-value: 7.4E-25 score: 87.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 266..437 e-value: 6.3E-44 score: 151.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 67..261 e-value: 9.8E-51 score: 174.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 86..436
None	No IPR available	PANTHER	PTHR47967:SF66	ASPARTIC PROTEINASE CDR1-RELATED	coord: 7..437
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 7..437
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 315..326
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 91..432 score: 44.546986
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 90..436 e-value: 2.53403E-73 score: 229.072

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS012863	MS012863	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS012863.1-cds	MS012863.1-cds-scaffold63:4085090..4086400	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS012863.1	MS012863.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity