MS012726.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS012726.1 vs. NCBI nr
Match: XP_022136886.1 (salicylate carboxymethyltransferase [Momordica charantia])

HSP 1 Score: 661.0 bits (1704), Expect = 5.4e-186
Identity = 326/331 (98.49%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISG PRPPPEFR
Sbjct: 36  KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGPPRPPPEFR 95

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYSS 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYSS
Sbjct: 96  VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYSS 155

Query: 121 YSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEVV 180
           YSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEVV
Sbjct: 156 YSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEVV 215

Query: 181 SGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPNEG 240
           SGGRMVLILLGRDGADHADRGNSFMWHLLAR LAILVSQGEIKEEKLDSYDVNFYAPNEG
Sbjct: 216 SGGRMVLILLGRDGADHADRGNSFMWHLLARALAILVSQGEIKEEKLDSYDVNFYAPNEG 275

Query: 241 EIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAILD 300
           EIE+EVRREGSFCLERIEKFE EKK KNNGDESYGREVAKAVRAIQETMISHHFGDAILD
Sbjct: 276 EIEDEVRREGSFCLERIEKFETEKKGKNNGDESYGREVAKAVRAIQETMISHHFGDAILD 335

Query: 301 SLFDKYGTILDEEMAKQEIRPISFVIVLRKL 332
           SLFDKYGTILDEEMAKQEIRPISFVIVLRKL
Sbjct: 336 SLFDKYGTILDEEMAKQEIRPISFVIVLRKL 366

BLAST of MS012726.1 vs. NCBI nr
Match: XP_038896307.1 (salicylate carboxymethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 7.4e-159
Identity = 284/333 (85.29%), Postives = 305/333 (91.59%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAI++ YLSTGAP SFG+ADLGCSSGPNSLSIIK+IIQAV+SL SG  +PP EFR
Sbjct: 33  KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIIKEIIQAVRSLSSGHIQPPLEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEG--ENPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFK LPDFCRELQNEG  +NPSGFFIGAYPGSFYQRLFPNN LHFVY
Sbjct: 93  VYLNDLPTNDFNSIFKTLPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNSLHFVY 152

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           SSYSLHWLSRVP+G+ DELGKP+NRGTIYISE SP ++AEAYV+QFR DF  FLR+RAEE
Sbjct: 153 SSYSLHWLSRVPEGVRDELGKPVNRGTIYISEKSPMSIAEAYVKQFRRDFWEFLRKRAEE 212

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDGADH DRGNSFMWHLLAR  AILVSQGEIKEEK+DSYDVNFYAPN
Sbjct: 213 VVSGGRMVLILLGRDGADHVDRGNSFMWHLLARAFAILVSQGEIKEEKVDSYDVNFYAPN 272

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAI 300
           + EIEEEV+REGSF LERIEKFE+EKKVKN   ESYG+EVAKAVRAIQE+MISHHFGD I
Sbjct: 273 KEEIEEEVKREGSFVLERIEKFELEKKVKNG--ESYGKEVAKAVRAIQESMISHHFGDEI 332

Query: 301 LDSLFDKYGTILDEEMAKQEIRPISFVIVLRKL 332
           LDSLF  YGTILDEEMAKQEI+PISFVIVL KL
Sbjct: 333 LDSLFQNYGTILDEEMAKQEIKPISFVIVLTKL 363

BLAST of MS012726.1 vs. NCBI nr
Match: XP_038896311.1 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 7.4e-159
Identity = 284/333 (85.29%), Postives = 305/333 (91.59%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAI++ YLSTGAP SFG+ADLGCSSGPNSLSIIK+IIQAV+SL SG  +PP EFR
Sbjct: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIIKEIIQAVRSLSSGHIQPPLEFR 62

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEG--ENPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFK LPDFCRELQNEG  +NPSGFFIGAYPGSFYQRLFPNN LHFVY
Sbjct: 63  VYLNDLPTNDFNSIFKTLPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNSLHFVY 122

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           SSYSLHWLSRVP+G+ DELGKP+NRGTIYISE SP ++AEAYV+QFR DF  FLR+RAEE
Sbjct: 123 SSYSLHWLSRVPEGVRDELGKPVNRGTIYISEKSPMSIAEAYVKQFRRDFWEFLRKRAEE 182

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDGADH DRGNSFMWHLLAR  AILVSQGEIKEEK+DSYDVNFYAPN
Sbjct: 183 VVSGGRMVLILLGRDGADHVDRGNSFMWHLLARAFAILVSQGEIKEEKVDSYDVNFYAPN 242

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAI 300
           + EIEEEV+REGSF LERIEKFE+EKKVKN   ESYG+EVAKAVRAIQE+MISHHFGD I
Sbjct: 243 KEEIEEEVKREGSFVLERIEKFELEKKVKNG--ESYGKEVAKAVRAIQESMISHHFGDEI 302

Query: 301 LDSLFDKYGTILDEEMAKQEIRPISFVIVLRKL 332
           LDSLF  YGTILDEEMAKQEI+PISFVIVL KL
Sbjct: 303 LDSLFQNYGTILDEEMAKQEIKPISFVIVLTKL 333

BLAST of MS012726.1 vs. NCBI nr
Match: XP_023553174.1 (salicylate carboxymethyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 564.3 bits (1453), Expect = 6.9e-157
Identity = 275/332 (82.83%), Postives = 305/332 (91.87%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAI++ YLSTGAP SFG+ADLGCSSGPNSLSI+K+IIQA+QSL S   +PPPEFR
Sbjct: 33  KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAIQSLSSSHLQPPPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEG--ENPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEG  +NPSGFFIGAYPGSFYQRLFPNNCLHFVY
Sbjct: 93  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFVY 152

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           SSYSLHWLSRVP+G+ +ELG+P+NRGTIYISE SP ++AEAYV+QFR DFC FLR+RAEE
Sbjct: 153 SSYSLHWLSRVPEGLRNELGEPLNRGTIYISERSPISIAEAYVKQFRRDFCEFLRKRAEE 212

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDGADH DRGNSF+WHLLAR  AILVSQG++KEE++D YDVNFYAPN
Sbjct: 213 VVSGGRMVLILLGRDGADHVDRGNSFLWHLLARAFAILVSQGKVKEEEVDRYDVNFYAPN 272

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAI 300
           + EIEEEV+REGSF LERIEKFE+EKKVKN   ESYG+EVAK VRAIQE+MISHHFGDAI
Sbjct: 273 KEEIEEEVKREGSFGLERIEKFELEKKVKNG--ESYGKEVAKTVRAIQESMISHHFGDAI 332

Query: 301 LDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           L+SLF  YGTILDEE AKQ+I+PISFVIVL K
Sbjct: 333 LESLFHNYGTILDEESAKQDIKPISFVIVLTK 362

BLAST of MS012726.1 vs. NCBI nr
Match: XP_008452093.1 (PREDICTED: salicylate carboxymethyltransferase [Cucumis melo])

HSP 1 Score: 563.9 bits (1452), Expect = 9.0e-157
Identity = 282/335 (84.18%), Postives = 303/335 (90.45%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAI++ YLSTGAPTSFG+ADLGCSSGPN+LSIIK+IIQAVQSL S   R  PEFR
Sbjct: 33  KHITMEAIEKVYLSTGAPTSFGIADLGCSSGPNTLSIIKEIIQAVQSLSSDHLRQSPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGE--NPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEGE  NPSGFFIGAYPGSFY+RLFP+N LHFV+
Sbjct: 93  VYLNDLPTNDFNSIFKALPDFCRELQNEGENQNPSGFFIGAYPGSFYKRLFPSNSLHFVH 152

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           SSYSLHWLSRVP+G+ DE GKP+NRGTIYISE SP ++ EAYV QFR DF  FLR+R EE
Sbjct: 153 SSYSLHWLSRVPEGVRDEFGKPVNRGTIYISERSPKSIVEAYVNQFRRDFWEFLRKRGEE 212

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDGADH DRGNSFMWHLLAR  AILVSQGE+KEEKLDSYDVNFYA N
Sbjct: 213 VVSGGRMVLILLGRDGADHVDRGNSFMWHLLARAFAILVSQGEVKEEKLDSYDVNFYAAN 272

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKV--KNNGDESYGREVAKAVRAIQETMISHHFGD 300
           + EIEEEVRREGSF LERIEKFE+EKKV  KNNGDESYG+EVAK+VRAIQE+MISHHFGD
Sbjct: 273 KEEIEEEVRREGSFGLERIEKFELEKKVRMKNNGDESYGKEVAKSVRAIQESMISHHFGD 332

Query: 301 AILDSLFDKYGTILDEEMAKQEIRPISFVIVLRKL 332
           +ILDSLF  YG ILDEEMAKQEIRPISFVIVL KL
Sbjct: 333 SILDSLFLNYGNILDEEMAKQEIRPISFVIVLTKL 367

BLAST of MS012726.1 vs. ExPASy Swiss-Prot
Match: A0A061DM17 (Probable methyltransferase TCM_000336 OS=Theobroma cacao OX=3641 GN=TCM_000336 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.2e-114
Identity = 205/319 (64.26%), Postives = 252/319 (79.00%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITME I+E Y S  AP S G+ADLGCSSGPNSLS+IKDI++ V+     L  P PEFR
Sbjct: 33  KHITMETIEELYYSI-APKSLGIADLGCSSGPNSLSLIKDIVEVVEETSHKLFHPLPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNE-GENPSGFFIGAYPGSFYQRLFPNNCLHFVYS 120
           VYLNDLPTNDFNS+FK+LPDF R+L+ +  E     FI  YPGSFY RLFPNNCLHF+YS
Sbjct: 93  VYLNDLPTNDFNSVFKSLPDFYRDLKKDRNEGGPAIFIAGYPGSFYGRLFPNNCLHFIYS 152

Query: 121 SYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEV 180
           SYSLHWLS+VP  + DE GK  N+G +YISE+SP +V++AY  QF+EDF +FL+ R+ EV
Sbjct: 153 SYSLHWLSKVPPALYDEHGKSTNKGNVYISESSPPSVSQAYWNQFQEDFSLFLKSRSVEV 212

Query: 181 VSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPNE 240
           V+GGRMVLILLGR G DH DRGNSF W +L+R+LAI VSQG+I  EKLDSY+V+FYAP+ 
Sbjct: 213 VTGGRMVLILLGRIGQDHVDRGNSFFWEILSRSLAISVSQGQIDREKLDSYEVHFYAPST 272

Query: 241 GEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAI- 300
            EIE+EV REGSF ++R+E FE++++ K+   ESYG  VA  VRAIQE+MI +HFGD I 
Sbjct: 273 NEIEDEVGREGSFEVDRLEMFEIDREAKDG--ESYGTAVAMTVRAIQESMICNHFGDGID 332

Query: 301 LDSLFDKYGTILDEEMAKQ 318
           LD+LF+ YG ++DEEMAKQ
Sbjct: 333 LDTLFNNYGKMVDEEMAKQ 348

BLAST of MS012726.1 vs. ExPASy Swiss-Prot
Match: A0A061FDP1 (Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 GN=TCM_034089 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.2e-73
Identity = 150/340 (44.12%), Postives = 210/340 (61.76%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           K I  EA+ E   +     S G+ADLGCSSGPN+L +I +I+  VQ+    L  P PEFR
Sbjct: 33  KPIIEEAVHEISCN-NVLESMGIADLGCSSGPNTLCVISEIMDMVQATSHRLGHPVPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNE-GENPSGFFIGAYPGSFYQRLFPNNCLHFVYS 120
           +YLNDL +NDFNSIF +LP F   L+ E G      FI    GSFY RLFP+  LH+V+S
Sbjct: 93  LYLNDLYSNDFNSIFMSLPAFYHRLKEEKGIGCGSCFISGVAGSFYGRLFPSKSLHYVHS 152

Query: 121 SYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEV 180
           S SLHWLS+VP G+      P+N+G +YIS++SP +V  AY  QF+ DF +F+  R++E+
Sbjct: 153 SSSLHWLSQVPPGLESNAVTPLNKGKVYISKSSPHSVLHAYSLQFQNDFPMFIESRSQEL 212

Query: 181 VSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPNE 240
           VSGGRMVL L GR   D     + + W LLA+ +  LV +G I+EEK+DS++  FYAP  
Sbjct: 213 VSGGRMVLSLFGRRSTDPTTEESCYQWELLAQAIMSLVREGLIEEEKVDSFNTPFYAPCA 272

Query: 241 GEIEEEVRREGSFCLERIEKFEME-------KKVKNNGDESYGREVAKAVRAIQETMISH 300
            EI+ E+++EGSF ++R+E FE++             G    G+  AKA+RA+ E+M+  
Sbjct: 273 EEIKVEIQKEGSFIIDRLEGFEIDWDGGAVSDVHTAQGKLLIGQRAAKALRAVVESMLES 332

Query: 301 HF--GDAILDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           HF  G  I+D LF +Y  I+   ++K   +  + VI L +
Sbjct: 333 HFGIGQDIMDDLFSRYAEIVGNHLSKTRTKYFNLVISLTR 371

BLAST of MS012726.1 vs. ExPASy Swiss-Prot
Match: A0A061FBW2 (Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 GN=TCM_034091 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.4e-72
Identity = 150/340 (44.12%), Postives = 211/340 (62.06%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           K I  EA+ E   +     S G+ADLGCSSGPN+LS+I +I+  VQ+    L RP PEFR
Sbjct: 33  KPIIEEAVHEISCN-NLLESMGIADLGCSSGPNTLSVISEIMDMVQTTSRRLGRPVPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNE-GENPSGFFIGAYPGSFYQRLFPNNCLHFVYS 120
           VYLNDL +NDFN IF +LP F   L+ E G      +I    GSFY RLFP+  LHFV+S
Sbjct: 93  VYLNDLYSNDFNYIFMSLPAFYHRLKEEKGIGCGSCYISGVAGSFYGRLFPSKSLHFVHS 152

Query: 121 SYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEV 180
           S SLHWLS+VP G+  +   P+N+G +YIS++SP +V  AY  QF+ DF +F+  R++E+
Sbjct: 153 SSSLHWLSQVPPGLESKALAPLNKGKVYISKSSPQSVLNAYSLQFQNDFSMFIESRSQEL 212

Query: 181 VSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPNE 240
           V GGRMVL  +GR   D     +   W LLA+ +  LV +G I+E K+DS++  +YAP  
Sbjct: 213 VPGGRMVLSFMGRRSTDPTTEESCHHWELLAQAIMSLVREGLIEEAKVDSFNAPYYAPCA 272

Query: 241 GEIEEEVRREGSFCLERIEKFEME------KKVKN-NGDESYGREVAKAVRAIQETMISH 300
            EI+ E+++ GSF ++R+E FE++        V+   G    G+ VAK +RA+ E+M+  
Sbjct: 273 EEIKVEIQKVGSFVIDRLEGFEIDWDGGAVSDVQTAQGKLLIGQRVAKTIRAVVESMLES 332

Query: 301 HF--GDAILDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           HF  G  I+D LF +Y  I+   ++K   + I+ VI L K
Sbjct: 333 HFGIGQDIMDDLFSRYAEIVGNHLSKTRTKYINLVISLIK 371

BLAST of MS012726.1 vs. ExPASy Swiss-Prot
Match: Q9SPV4 (Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.6e-69
Identity = 142/335 (42.39%), Postives = 208/335 (62.09%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRP-PPEF 60
           K IT  AI   Y      T   +ADLGCSSGPN+L  + ++I+ V+ L   + R   PE+
Sbjct: 33  KPITEAAITALYSGDTVTTRLAIADLGCSSGPNALFAVTELIKTVEELRKKMGRENSPEY 92

Query: 61  RVYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYS 120
           +++LNDLP NDFN+IF++LP     ++N+ +     FI   PGSFY RLFP N LHF++S
Sbjct: 93  QIFLNDLPGNDFNAIFRSLP-----IENDVDGVC--FINGVPGSFYGRLFPRNTLHFIHS 152

Query: 121 SYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEV 180
           SYSL WLS+VP GI        N+G IY++   P +V  AY +QF+ED  +FLR RA+EV
Sbjct: 153 SYSLMWLSQVPIGI------ESNKGNIYMANTCPQSVLNAYYKQFQEDHALFLRCRAQEV 212

Query: 181 VSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPNE 240
           V GGRMVL +LGR   D A      +W LLA  L  +VS+G I+EEK+D +++  Y P+ 
Sbjct: 213 VPGGRMVLTILGRRSEDRASTECCLIWQLLAMALNQMVSEGLIEEEKMDKFNIPQYTPSP 272

Query: 241 GEIEEEVRREGSFCLERIEKFEME-KKVKNNGD-----ESYGREVAKAVRAIQETMISHH 300
            E+E E+ +EGSF ++ IE  E+       +GD     E  G  VA+ +RA+ E ++  H
Sbjct: 273 TEVEAEILKEGSFLIDHIEASEIYWSSCTKDGDGGGSVEEEGYNVARCMRAVAEPLLLDH 332

Query: 301 FGDAILDSLFDKYGTILDEEMAKQEIRPISFVIVL 329
           FG+AI++ +F +Y  ++ E M+K++ + I+ ++ L
Sbjct: 333 FGEAIIEDVFHRYKLLIIERMSKEKTKFINVIVSL 354

BLAST of MS012726.1 vs. ExPASy Swiss-Prot
Match: Q9SBK6 (Jasmonate O-methyltransferase OS=Brassica rapa subsp. pekinensis OX=51351 GN=JMT PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 9.6e-69
Identity = 141/343 (41.11%), Postives = 202/343 (58.89%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           + +  EA+++  +      SFG+ADLGCSSGPNSL  I +I++ +Q+L   L RP PE  
Sbjct: 33  RRVMDEALKKLMIRNSEILSFGIADLGCSSGPNSLLSISNIVETIQNLCHDLDRPVPELS 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGEN--PSGF--------FIGAYPGSFYQRLFP 120
           + LNDLP+NDFN IF +LP+F   ++    N    GF        F+ A PGSFY RLFP
Sbjct: 93  LSLNDLPSNDFNYIFASLPEFYDRVKKRDNNYESLGFEHGSGGPCFVSAVPGSFYGRLFP 152

Query: 121 NNCLHFVYSSYSLHWLSRVPDGICDELGKP---------INRGTIYISEASPGAVAEAYV 180
              LHFV+SS SLHWLS+VP   C E+ K           NRG IY+S+ SP +  + Y 
Sbjct: 153 RRSLHFVHSSSSLHWLSQVP---CGEVNKKDGVVITADLDNRGKIYLSKTSPKSAHKVYA 212

Query: 181 RQFREDFCVFLRRRAEEVVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGE 240
            QF+ DF VFLR R+EE+V GGRMVL  LGR   D     + + W LLA+ L  L  +G 
Sbjct: 213 LQFQTDFSVFLRSRSEELVPGGRMVLSFLGRSSPDPTTEESCYQWELLAQALMSLAKEGI 272

Query: 241 IKEEKLDSYDVNFYAPNEGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESY------- 300
           I+EE +D+++  +YA +  E++  + +EGSF ++R+E   ++ +  +  D+SY       
Sbjct: 273 IEEENIDAFNAPYYAASPEELKMAIEKEGSFSIDRLEISPVDWEGGSISDDSYDIVRFKP 332

Query: 301 -----GREVAKAVRAIQETMISHHFGDAILDSLFDKYGTILDE 313
                GR VAK +RA+ E M+   FG  ++D LF++Y  ++ E
Sbjct: 333 EALASGRRVAKTIRAVVEPMLEPTFGQKVMDELFERYAKLVGE 372

BLAST of MS012726.1 vs. ExPASy TrEMBL
Match: A0A6J1C4R9 (salicylate carboxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111008468 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.6e-186
Identity = 326/331 (98.49%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISG PRPPPEFR
Sbjct: 36  KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGPPRPPPEFR 95

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYSS 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYSS
Sbjct: 96  VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYSS 155

Query: 121 YSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEVV 180
           YSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEVV
Sbjct: 156 YSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEVV 215

Query: 181 SGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPNEG 240
           SGGRMVLILLGRDGADHADRGNSFMWHLLAR LAILVSQGEIKEEKLDSYDVNFYAPNEG
Sbjct: 216 SGGRMVLILLGRDGADHADRGNSFMWHLLARALAILVSQGEIKEEKLDSYDVNFYAPNEG 275

Query: 241 EIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAILD 300
           EIE+EVRREGSFCLERIEKFE EKK KNNGDESYGREVAKAVRAIQETMISHHFGDAILD
Sbjct: 276 EIEDEVRREGSFCLERIEKFETEKKGKNNGDESYGREVAKAVRAIQETMISHHFGDAILD 335

Query: 301 SLFDKYGTILDEEMAKQEIRPISFVIVLRKL 332
           SLFDKYGTILDEEMAKQEIRPISFVIVLRKL
Sbjct: 336 SLFDKYGTILDEEMAKQEIRPISFVIVLRKL 366

BLAST of MS012726.1 vs. ExPASy TrEMBL
Match: A0A1S3BSG5 (salicylate carboxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103493203 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.4e-157
Identity = 282/335 (84.18%), Postives = 303/335 (90.45%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAI++ YLSTGAPTSFG+ADLGCSSGPN+LSIIK+IIQAVQSL S   R  PEFR
Sbjct: 33  KHITMEAIEKVYLSTGAPTSFGIADLGCSSGPNTLSIIKEIIQAVQSLSSDHLRQSPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGE--NPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEGE  NPSGFFIGAYPGSFY+RLFP+N LHFV+
Sbjct: 93  VYLNDLPTNDFNSIFKALPDFCRELQNEGENQNPSGFFIGAYPGSFYKRLFPSNSLHFVH 152

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           SSYSLHWLSRVP+G+ DE GKP+NRGTIYISE SP ++ EAYV QFR DF  FLR+R EE
Sbjct: 153 SSYSLHWLSRVPEGVRDEFGKPVNRGTIYISERSPKSIVEAYVNQFRRDFWEFLRKRGEE 212

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDGADH DRGNSFMWHLLAR  AILVSQGE+KEEKLDSYDVNFYA N
Sbjct: 213 VVSGGRMVLILLGRDGADHVDRGNSFMWHLLARAFAILVSQGEVKEEKLDSYDVNFYAAN 272

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKV--KNNGDESYGREVAKAVRAIQETMISHHFGD 300
           + EIEEEVRREGSF LERIEKFE+EKKV  KNNGDESYG+EVAK+VRAIQE+MISHHFGD
Sbjct: 273 KEEIEEEVRREGSFGLERIEKFELEKKVRMKNNGDESYGKEVAKSVRAIQESMISHHFGD 332

Query: 301 AILDSLFDKYGTILDEEMAKQEIRPISFVIVLRKL 332
           +ILDSLF  YG ILDEEMAKQEIRPISFVIVL KL
Sbjct: 333 SILDSLFLNYGNILDEEMAKQEIRPISFVIVLTKL 367

BLAST of MS012726.1 vs. ExPASy TrEMBL
Match: A0A6J1JAG4 (salicylate carboxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482729 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.1e-155
Identity = 274/332 (82.53%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAI++ YLSTG P SFG+ADLGCSSGPNSLSI+K+IIQAVQSL S   +PPPEFR
Sbjct: 33  KHITMEAIEQVYLSTGTPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEG--ENPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEG  +NPSGFFIGAYPGSFYQRLFPNNCLHFVY
Sbjct: 93  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFVY 152

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           SSYSLHWLSRVP+G+ +ELG+P+NRGTIYISE SP ++AEAYV+QFR DF  FLR+RAEE
Sbjct: 153 SSYSLHWLSRVPEGLRNELGEPLNRGTIYISERSPISIAEAYVKQFRRDFWEFLRKRAEE 212

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDGADH DRGNSF+WHLLAR  AILVSQG++KEE++D YDVNFYAPN
Sbjct: 213 VVSGGRMVLILLGRDGADHVDRGNSFLWHLLARAFAILVSQGKVKEEEVDRYDVNFYAPN 272

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAI 300
           + EIEEEV+REGSF LERIEKFE+EKKVKN+  ESYG+EVAK VRAIQE+MISHHFGDAI
Sbjct: 273 KEEIEEEVKREGSFGLERIEKFELEKKVKNS--ESYGKEVAKTVRAIQESMISHHFGDAI 332

Query: 301 LDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           L+SLF  YGTILDEE AKQ+I+PISFVIVL K
Sbjct: 333 LESLFHNYGTILDEESAKQDIKPISFVIVLTK 362

BLAST of MS012726.1 vs. ExPASy TrEMBL
Match: A0A6J1EMX7 (salicylate carboxymethyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111436043 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.4e-155
Identity = 274/332 (82.53%), Postives = 303/332 (91.27%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KHITMEAI++ YLSTGAP SFG+ADLGCSSGPNSLSI+K+IIQAVQSL S   +PPPEFR
Sbjct: 33  KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEG--ENPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEG  +NPSGFFIGAYPGSFYQRLFPNNCLHFVY
Sbjct: 93  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFVY 152

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           SSYSLHWLSRVP+G+ +ELG+P+NRGTIYISE SP ++AEAYV+QFR DF  FLR+RAEE
Sbjct: 153 SSYSLHWLSRVPEGLRNELGEPLNRGTIYISERSPISIAEAYVKQFRRDFGEFLRKRAEE 212

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDGADH DRGNSF+WHLLAR  AILVSQG++KEE++D YDVNFYAPN
Sbjct: 213 VVSGGRMVLILLGRDGADHVDRGNSFLWHLLARAFAILVSQGKVKEEEVDRYDVNFYAPN 272

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDAI 300
           + EIEEEV+REGSF LERIEKFE+EKKVKN   ESYG+EVAK VRAIQE+MISHHFGDAI
Sbjct: 273 KDEIEEEVKREGSFGLERIEKFELEKKVKNG--ESYGKEVAKTVRAIQESMISHHFGDAI 332

Query: 301 LDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           L+SLF  Y TILDEE AKQ+I+PISFVIVL K
Sbjct: 333 LESLFHNYSTILDEESAKQDIKPISFVIVLTK 362

BLAST of MS012726.1 vs. ExPASy TrEMBL
Match: A0A0A0KUR7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G046610 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.5e-154
Identity = 273/336 (81.25%), Postives = 302/336 (89.88%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           KH+TMEAI++ YLSTGAPTSFG+ADLGCSSGPN+LSI+K+IIQA QSL S   R   EFR
Sbjct: 33  KHVTMEAIEKVYLSTGAPTSFGIADLGCSSGPNTLSIVKEIIQAFQSLSSDHLRQSSEFR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEG--ENPSGFFIGAYPGSFYQRLFPNNCLHFVY 120
           VYLNDLPTNDFNSIFKALPDFCRELQNEG  +NPSGFFIGAYPGSFYQRLFP+NCLHFVY
Sbjct: 93  VYLNDLPTNDFNSIFKALPDFCRELQNEGVNQNPSGFFIGAYPGSFYQRLFPSNCLHFVY 152

Query: 121 SSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEE 180
           S+YSLHWLSRVP+G+ DE GKP+NRGTIYISE SP +V EAYV+QFR DF  FLR+RAEE
Sbjct: 153 SNYSLHWLSRVPEGVRDEFGKPVNRGTIYISERSPISVVEAYVKQFRRDFWEFLRKRAEE 212

Query: 181 VVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPN 240
           VVSGGRMVLILLGRDG DH DRGNSFMWHLLA   AILVS+GE+KEE+LDSYDVNFYA N
Sbjct: 213 VVSGGRMVLILLGRDGTDHVDRGNSFMWHLLAEAFAILVSKGEVKEEELDSYDVNFYAAN 272

Query: 241 EGEIEEEVRREGSFCLERIEKFEMEKKVK---NNGDESYGREVAKAVRAIQETMISHHFG 300
           + EIEEEVRREGSF LERIEKFE+EKKVK   N GDESYG+EVAK+VRAIQE+MISHHFG
Sbjct: 273 KEEIEEEVRREGSFGLERIEKFELEKKVKMNNNGGDESYGKEVAKSVRAIQESMISHHFG 332

Query: 301 DAILDSLFDKYGTILDEEMAKQEIRPISFVIVLRKL 332
           D+ILDSLF  YGT+LDEEMAKQEI+PI+FV+VL KL
Sbjct: 333 DSILDSLFLNYGTLLDEEMAKQEIKPITFVLVLTKL 368

BLAST of MS012726.1 vs. TAIR 10
Match: AT4G36470.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 4.4e-109
Identity = 201/336 (59.82%), Postives = 256/336 (76.19%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQ-SLISGLP-RPPPE 60
           KHIT+E +Q+ Y  T  P S G+ADLGCSSGPN+LS I D I+ VQ +    +P +P PE
Sbjct: 36  KHITLETLQQLYKET-RPKSLGIADLGCSSGPNTLSTITDFIKTVQVAHHREIPIQPLPE 95

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQ---NEGENPSGFFIGAYPGSFYQRLFPNNCLH 120
           F ++LNDLP NDFN IFK+LPDF  EL+   N G+ PS  FI AYPGSFY RLFP N +H
Sbjct: 96  FSIFLNDLPGNDFNFIFKSLPDFHIELKRDNNNGDCPS-VFIAAYPGSFYGRLFPENTIH 155

Query: 121 FVYSSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRR 180
           FVY+S+SLHWLS+VP  + DE GK IN+G + I   S  AV++AY  QF+EDF +FLR R
Sbjct: 156 FVYASHSLHWLSKVPTALYDEQGKSINKGCVSICSLSSEAVSKAYCSQFKEDFSIFLRCR 215

Query: 181 AEEVVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFY 240
           ++E+VS GRMVLI+LGR+G DH DRGNSF W LL+R++A LV+QGE +EEKLDSYD++FY
Sbjct: 216 SKEMVSAGRMVLIILGREGPDHVDRGNSFFWELLSRSIADLVAQGETEEEKLDSYDMHFY 275

Query: 241 APNEGEIEEEVRREGSFCLERIEKFEMEK-KVKNNGDESYGREVAKAVRAIQETMISHHF 300
           AP+  EIE EV +EGSF LER+E  E++K K    GD SYG+ VAK VRA+QE+M+  HF
Sbjct: 276 APSADEIEGEVDKEGSFELERLEMLEVKKDKGNTEGDISYGKAVAKTVRAVQESMLVQHF 335

Query: 301 GDAILDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           G+ ILD LFD Y  ++D+E+AK++IRPI+FV+VLRK
Sbjct: 336 GEKILDKLFDTYCRMVDDELAKEDIRPITFVVVLRK 369

BLAST of MS012726.1 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 259.2 bits (661), Expect = 4.4e-69
Identity = 140/338 (41.42%), Postives = 202/338 (59.76%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           + +  EA+++  +S    +S G+ADLGCSSGPNSL  I +I+  + +L   L RP PE R
Sbjct: 33  RRVMDEALKKLMMSNSEISSIGIADLGCSSGPNSLLSISNIVDTIHNLCPDLDRPVPELR 92

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGF--------FIGAYPGSFYQRLFPNN 120
           V LNDLP+NDFN I  +LP+F   + N  E   GF        F+ A PGSFY RLFP  
Sbjct: 93  VSLNDLPSNDFNYICASLPEFYDRVNNNKEG-LGFGRGGGESCFVSAVPGSFYGRLFPRR 152

Query: 121 CLHFVYSSYSLHWLSRVPDGICDELGKPI-----NRGTIYISEASPGAVAEAYVRQFRED 180
            LHFV+SS SLHWLS+VP    ++  + I     N G IYIS+ SP +  +AY  QF+ D
Sbjct: 153 SLHFVHSSSSLHWLSQVPCREAEKEDRTITADLENMGKIYISKTSPKSAHKAYALQFQTD 212

Query: 181 FCVFLRRRAEEVVSGGRMVLILLGRDGADHADRGNSFMWHLLARTLAILVSQGEIKEEKL 240
           F VFLR R+EE+V GGRMVL  LGR   D     + + W LLA+ L  +  +G I+EEK+
Sbjct: 213 FWVFLRSRSEELVPGGRMVLSFLGRRSLDPTTEESCYQWELLAQALMSMAKEGIIEEEKI 272

Query: 241 DSYDVNFYAPNEGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDESY------------- 300
           D+++  +YA +  E++  + +EGSF ++R+E   ++ +  +  +ESY             
Sbjct: 273 DAFNAPYYAASSEELKMVIEKEGSFSIDRLEISPIDWEGGSISEESYDLVIRSKPEALAS 332

Query: 301 GREVAKAVRAIQETMISHHFGDAILDSLFDKYGTILDE 313
           GR V+  +RA+ E M+   FG+ ++D LF++Y  I+ E
Sbjct: 333 GRRVSNTIRAVVEPMLEPTFGENVMDELFERYAKIVGE 369

BLAST of MS012726.1 vs. TAIR 10
Match: AT3G11480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 240.4 bits (612), Expect = 2.1e-63
Identity = 133/333 (39.94%), Postives = 199/333 (59.76%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           K + +   +E  ++   PT   VA+LGCSSG NS   I +II  +  L   + +  PE  
Sbjct: 55  KPVLVRNTEEMMMNLDFPTYIKVAELGCSSGQNSFLAIFEIINTINVLCQHVNKNSPEID 114

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGENPSGFFIGAYPGSFYQRLFPNNCLHFVYSS 120
             LNDLP NDFN+ FK +P F +EL     N S  F+   PGSFY RLF  N LH ++SS
Sbjct: 115 CCLNDLPENDFNTTFKFVPFFNKELMI--TNKSSCFVYGAPGSFYSRLFSRNSLHLIHSS 174

Query: 121 YSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAEEVV 180
           Y+LHWLS+VP+ + +      N+G +YI+ +SP +  +AY+ QF++DF +FLR R+EE+V
Sbjct: 175 YALHWLSKVPEKLEN------NKGNLYITSSSPQSAYKAYLNQFQKDFTMFLRLRSEEIV 234

Query: 181 SGGRMVLILLGRDGA-DHADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYAPNE 240
           S GRMVL  +GR+   D   R     W LL+ +L  LV +G + E KLD++++ FY PN 
Sbjct: 235 SNGRMVLTFIGRNTLNDPLYRDCCHFWTLLSNSLRDLVFEGLVSESKLDAFNMPFYDPNV 294

Query: 241 GEIEEEVRREGSFCLERIEK--FEMEKKVKNNGDESYGREVAKAVRAIQETMISHHFGDA 300
            E++E +++EGSF +  +E   F++    + +  E+ GR  A  +RA+ E M+  HFG+ 
Sbjct: 295 QELKEVIQKEGSFEINELESHGFDLGHYYEEDDFEA-GRNEANGIRAVSEPMLIAHFGEE 354

Query: 301 ILDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           I+D+LFDKY   + +    +    +S V+ L K
Sbjct: 355 IIDTLFDKYAYHVTQHANCRNKTTVSLVVSLTK 378

BLAST of MS012726.1 vs. TAIR 10
Match: AT5G04370.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.8e-60
Identity = 130/338 (38.46%), Postives = 198/338 (58.58%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           K + ++  ++  ++   PT   VADLGCSSG N+   + +II  +        + PPE  
Sbjct: 65  KPVLVKNTKDLMINLNFPTYIKVADLGCSSGQNTFLAMSEIINTINVFCQQRNQNPPEID 124

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGEN---PSGFFIGAYPGSFYQRLFPNNCLHFV 120
             LNDLP+NDFN+ FK +  F       G N      +F+   PGSFY RLFP   LHFV
Sbjct: 125 CCLNDLPSNDFNTTFKFIQFF------NGMNITSKESYFVYGVPGSFYSRLFPRRSLHFV 184

Query: 121 YSSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAE 180
           +SSY LHWLS+VP+G+        N+ ++YI+ +SP +  +AY+ QF+ DF  FL+ R+E
Sbjct: 185 HSSYGLHWLSKVPEGL------EKNKMSVYITNSSPLSTYKAYLNQFQRDFATFLKLRSE 244

Query: 181 EVVSGGRMVLILLGRDGADH-ADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYA 240
           E+VS GRMVL  +GR+  D+   R     W LL+++L  LV++G +   K+DS+ + FY 
Sbjct: 245 EMVSNGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDLVAEGLVSASKVDSFYLPFYD 304

Query: 241 PNEGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDES----YGREVAKAVRAIQETMISH 300
           PNE EI+E V++EGSF +  +E    +     N DES     G+  A  +RA+ E +++ 
Sbjct: 305 PNEKEIKEMVQKEGSFEIRDLETHGYDLG-HCNQDESKRSKSGQNEANYIRAVSEPLLAA 364

Query: 301 HFGDAILDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           HFGDAI++ LF+K+   + + ++ +    +S V+ L K
Sbjct: 365 HFGDAIINILFNKFACHVSQHVSCRNKTTVSIVVSLTK 389

BLAST of MS012726.1 vs. TAIR 10
Match: AT5G04370.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.8e-60
Identity = 130/338 (38.46%), Postives = 198/338 (58.58%), Query Frame = 0

Query: 1   KHITMEAIQEAYLSTGAPTSFGVADLGCSSGPNSLSIIKDIIQAVQSLISGLPRPPPEFR 60
           K + ++  ++  ++   PT   VADLGCSSG N+   + +II  +        + PPE  
Sbjct: 84  KPVLVKNTKDLMINLNFPTYIKVADLGCSSGQNTFLAMSEIINTINVFCQQRNQNPPEID 143

Query: 61  VYLNDLPTNDFNSIFKALPDFCRELQNEGEN---PSGFFIGAYPGSFYQRLFPNNCLHFV 120
             LNDLP+NDFN+ FK +  F       G N      +F+   PGSFY RLFP   LHFV
Sbjct: 144 CCLNDLPSNDFNTTFKFIQFF------NGMNITSKESYFVYGVPGSFYSRLFPRRSLHFV 203

Query: 121 YSSYSLHWLSRVPDGICDELGKPINRGTIYISEASPGAVAEAYVRQFREDFCVFLRRRAE 180
           +SSY LHWLS+VP+G+        N+ ++YI+ +SP +  +AY+ QF+ DF  FL+ R+E
Sbjct: 204 HSSYGLHWLSKVPEGL------EKNKMSVYITNSSPLSTYKAYLNQFQRDFATFLKLRSE 263

Query: 181 EVVSGGRMVLILLGRDGADH-ADRGNSFMWHLLARTLAILVSQGEIKEEKLDSYDVNFYA 240
           E+VS GRMVL  +GR+  D+   R     W LL+++L  LV++G +   K+DS+ + FY 
Sbjct: 264 EMVSNGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDLVAEGLVSASKVDSFYLPFYD 323

Query: 241 PNEGEIEEEVRREGSFCLERIEKFEMEKKVKNNGDES----YGREVAKAVRAIQETMISH 300
           PNE EI+E V++EGSF +  +E    +     N DES     G+  A  +RA+ E +++ 
Sbjct: 324 PNEKEIKEMVQKEGSFEIRDLETHGYDLG-HCNQDESKRSKSGQNEANYIRAVSEPLLAA 383

Query: 301 HFGDAILDSLFDKYGTILDEEMAKQEIRPISFVIVLRK 331
           HFGDAI++ LF+K+   + + ++ +    +S V+ L K
Sbjct: 384 HFGDAIINILFNKFACHVSQHVSCRNKTTVSIVVSLTK 408