MS012332.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS012332.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	proline-rich receptor-like protein kinase PERK2
Location	scaffold63: 38463 .. 38774 (+)
Sequence length	312
RNA-Seq Expression	MS012332.1
Synteny	MS012332.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTTCCAAGTTCTTGATCATTGCCTTATTCATGGTCGCTTTGGCACTTTCGAGCATCGACGCCGCCCGAGCTGCCCGGCATCTTCTGCAAACTGCTCCACCCTCGCTGCCCAACTTCCCGGTGGTGCCAACGGGGCTCCCGCCAATGCCGATAACGACCTTGCCAAACCCTACTACAGGGCCGAGTATTATTCCTTCTCTTCCAAATCTGCCTCAGCCCACATTGCCTAGGGTTACACTTCCTCCGTTCCCAAGCTTCCCGGTGAACATCCCATTTCTCGCGCCTCCACCAGCAACTTCTTCACCC

mRNA sequence

ATGGCTTCTTCCAAGTTCTTGATCATTGCCTTATTCATGGTCGCTTTGGCACTTTCGAGCATCGACGCCGCCCGAGCTGCCCGGCATCTTCTGCAAACTGCTCCACCCTCGCTGCCCAACTTCCCGGTGGTGCCAACGGGGCTCCCGCCAATGCCGATAACGACCTTGCCAAACCCTACTACAGGGCCGAGTATTATTCCTTCTCTTCCAAATCTGCCTCAGCCCACATTGCCTAGGGTTACACTTCCTCCGTTCCCAAGCTTCCCGGTGAACATCCCATTTCTCGCGCCTCCACCAGCAACTTCTTCACCC

Coding sequence (CDS)

ATGGCTTCTTCCAAGTTCTTGATCATTGCCTTATTCATGGTCGCTTTGGCACTTTCGAGCATCGACGCCGCCCGAGCTGCCCGGCATCTTCTGCAAACTGCTCCACCCTCGCTGCCCAACTTCCCGGTGGTGCCAACGGGGCTCCCGCCAATGCCGATAACGACCTTGCCAAACCCTACTACAGGGCCGAGTATTATTCCTTCTCTTCCAAATCTGCCTCAGCCCACATTGCCTAGGGTTACACTTCCTCCGTTCCCAAGCTTCCCGGTGAACATCCCATTTCTCGCGCCTCCACCAGCAACTTCTTCACCC

Protein sequence

MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPTTGPSIIPSLPNLPQPTLPRVTLPPFPSFPVNIPFLAPPPATSSP

Homology

[Close]

BLAST of MS012332.1 vs. NCBI nr
Match: XP_022149166.1 (proline-rich receptor-like protein kinase PERK2 [Momordica charantia])

HSP 1 Score: 174.1 bits (440), Expect = 6.3e-40
Identity = 100/116 (86.21%), Postives = 101/116 (87.07%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIALFMVALALSS+DAARAARHLLQTAPPSLPNFPVVP GLPPMPI TLPNPT
Sbjct: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPFPSFPVN------------IPFLAPPPATSSP 105
           TGPSIIPSLPNLPQPTLPRVTLPP PSFPVN            IPFLAPPPATSSP
Sbjct: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP 116

[Close]

BLAST of MS012332.1 vs. NCBI nr
Match: XP_038904303.1 (protein PELPK1-like [Benincasa hispida])

HSP 1 Score: 109.8 bits (273), Expect = 1.5e-20
Identity = 75/126 (59.52%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           M SSKFLIIA FM  LALSSID  +AARHLLQTAPP +PN PV+PTG+PP+P  TLPNPT
Sbjct: 1   MGSSKFLIIA-FMATLALSSIDLGQAARHLLQTAPPLVPNLPVIPTGIPPLP--TLPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPP-----------------FPSFPV------NIPFLAP 104
           T       LPNLPQPTLP+V LPP                 FPS P       +IPFL+P
Sbjct: 61  T-------LPNLPQPTLPKVNLPPLPSGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 116

[Close]

BLAST of MS012332.1 vs. NCBI nr
Match: KAA0056641.1 (proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK07567.1 proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 106.7 bits (265), Expect = 1.2e-19
Identity = 74/133 (55.64%), Postives = 81/133 (60.90%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIA FM  LALSSID  +AARHLLQTAPP +PN PV+PTG+PP+P   L NPT
Sbjct: 1   MASSKFLIIA-FMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIPPLP--ALSNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPFPSFPVN----------------------------- 104
                   LPNLPQPTLP+V LPP PS PVN                             
Sbjct: 61  --------LPNLPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFP 120

[Close]

BLAST of MS012332.1 vs. NCBI nr
Match: KAG6577257.1 (hypothetical protein SDJN03_24831, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015347.1 hypothetical protein SDJN02_22982, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 105.1 bits (261), Expect = 3.6e-19
Identity = 70/128 (54.69%), Postives = 77/128 (60.16%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIA FM  LALSSID  +AARHLLQT PP LPN P +PTG+PP          
Sbjct: 1   MASSKFLIIA-FMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPP---------- 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPFPSFPVN-------------------------IPFL 104
                +P+LPNLPQPTLP+V LPPFPS PVN                         IPFL
Sbjct: 61  -----LPTLPNLPQPTLPKVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFPSIPFL 112

[Close]

BLAST of MS012332.1 vs. NCBI nr
Match: KAA0056642.1 (proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 100.9 bits (250), Expect = 6.8e-18
Identity = 70/126 (55.56%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIA FM  LALSSI+  +AARHLLQT PP +PN P +PTG+PP P   LPNPT
Sbjct: 1   MASSKFLIIA-FMATLALSSINFGQAARHLLQTTPPLMPNLPAIPTGIPPFP--ALPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPP-----------------FPSFPV------NIPFLAP 104
                   LPNLPQPT+P+V LPP                 FPS P       +IPFL+P
Sbjct: 61  --------LPNLPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 115

[Close]

BLAST of MS012332.1 vs. ExPASy TrEMBL
Match: A0A6J1D7J7 (proline-rich receptor-like protein kinase PERK2 OS=Momordica charantia OX=3673 GN=LOC111017657 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.1e-40
Identity = 100/116 (86.21%), Postives = 101/116 (87.07%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIALFMVALALSS+DAARAARHLLQTAPPSLPNFPVVP GLPPMPI TLPNPT
Sbjct: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPFPSFPVN------------IPFLAPPPATSSP 105
           TGPSIIPSLPNLPQPTLPRVTLPP PSFPVN            IPFLAPPPATSSP
Sbjct: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP 116

[Close]

BLAST of MS012332.1 vs. ExPASy TrEMBL
Match: A0A5D3C6P2 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00400 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.0e-20
Identity = 74/133 (55.64%), Postives = 81/133 (60.90%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIA FM  LALSSID  +AARHLLQTAPP +PN PV+PTG+PP+P   L NPT
Sbjct: 1   MASSKFLIIA-FMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIPPLP--ALSNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPFPSFPVN----------------------------- 104
                   LPNLPQPTLP+V LPP PS PVN                             
Sbjct: 61  --------LPNLPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFP 120

[Close]

BLAST of MS012332.1 vs. ExPASy TrEMBL
Match: A0A5A7ULD9 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold288G001670 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.3e-18
Identity = 70/126 (55.56%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIA FM  LALSSI+  +AARHLLQT PP +PN P +PTG+PP P   LPNPT
Sbjct: 1   MASSKFLIIA-FMATLALSSINFGQAARHLLQTTPPLMPNLPAIPTGIPPFP--ALPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPP-----------------FPSFPV------NIPFLAP 104
                   LPNLPQPT+P+V LPP                 FPS P       +IPFL+P
Sbjct: 61  --------LPNLPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 115

[Close]

BLAST of MS012332.1 vs. ExPASy TrEMBL
Match: A0A5D3C8R8 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00410 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 4.3e-18
Identity = 70/126 (55.56%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIA FM  LALSSI+  +AARHLLQT PP +PN P +PTG+PP P   LPNPT
Sbjct: 1   MASSKFLIIA-FMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIPPFP--ALPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPP-----------------FPSFPV------NIPFLAP 104
                   LPNLPQPT+P+V LPP                 FPS P       +IPFL+P
Sbjct: 61  --------LPNLPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 115

[Close]

BLAST of MS012332.1 vs. ExPASy TrEMBL
Match: A0A1S3CJJ1 (proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC103501166 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 4.3e-18
Identity = 70/126 (55.56%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAARHLLQTAPPSLPNFPVVPTGLPPMPITTLPNPT 60
           MASSKFLIIA FM  LALSSI+  +AARHLLQT PP +PN P +PTG+PP P   LPNPT
Sbjct: 1   MASSKFLIIA-FMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIPPFP--ALPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPP-----------------FPSFPV------NIPFLAP 104
                   LPNLPQPT+P+V LPP                 FPS P       +IPFL+P
Sbjct: 61  --------LPNLPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 115

[Close]

BLAST of MS012332.1 vs. TAIR 10
Match: AT4G38080.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 48.5 bits (114), Expect = 3.7e-06
Identity = 50/127 (39.37%), Postives = 64/127 (50.39%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSIDAARAA-RHLLQ----TAPPSLPNFPVVPTGLPPMPI-- 60
           MA  K   +   ++AL  +S   + +A RHLLQ    T PP+L   P+  T +PP+P   
Sbjct: 1   MAPLKNSFVTSLVIALTFTSFFTSLSAHRHLLQSTPVTQPPALTFPPLPKTTMPPVPSLP 60

Query: 61  -----------TTLPNPTTGPSI----IPSLPNLPQPTLPRVTLPPFPS-----FPVNIP 101
                       TLP PT  P +    IPSLPN  QPT+P +    FPS     FP NIP
Sbjct: 61  TPGQQTLPQPQPTLPQPTGLPPMPSTQIPSLPNQVQPTIPNIPQINFPSNFPFNFPFNIP 120

[Close]

BLAST of MS012332.1 vs. TAIR 10
Match: AT2G22510.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 42.0 bits (97), Expect = 3.5e-04
Identity = 46/119 (38.66%), Postives = 61/119 (51.26%), Query Frame = 0


Query: 9   IALFMVALALSS-IDAARAARHLLQT----APPSLPNFPVVP-TGLPPM-PITTLPNPTT 68
           ++  ++AL  +S      A+RHLLQ+     PP    FP +P T +PP  P T+LP PT 
Sbjct: 5   LSSLVIALTFTSFFTGLSASRHLLQSTPAITPPVTTTFPPLPTTTMPPFPPSTSLPQPTA 64

Query: 69  GP----SIIPSLPNLPQ----PTLPRVTLPPFP-----SFPVN-------IPFLAPPPA 101
            P    S IPSLPN  Q    P  P++ +P FP     +FP N       IPF  PPP+
Sbjct: 65  FPPLPSSQIPSLPNPAQPINIPNFPQINIPNFPISIPNNFPFNLPTSIPTIPFFTPPPS 123

The following BLAST results are available for this feature:

BLAST of MS012332.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149166.1	6.3e-40	86.21	proline-rich receptor-like protein kinase PERK2 [Momordica charantia]	[more]
XP_038904303.1	1.5e-20	59.52	protein PELPK1-like [Benincasa hispida]	[more]
KAA0056641.1	1.2e-19	55.64	proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK0...	[more]
KAG6577257.1	3.6e-19	54.69	hypothetical protein SDJN03_24831, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAA0056642.1	6.8e-18	55.56	proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa]	[more]

BLAST of MS012332.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS012332.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7J7	3.1e-40	86.21	proline-rich receptor-like protein kinase PERK2 OS=Momordica charantia OX=3673 G...	[more]
A0A5D3C6P2	6.0e-20	55.64	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5A7ULD9	3.3e-18	55.56	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3C8R8	4.3e-18	55.56	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3CJJ1	4.3e-18	55.56	proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of MS012332.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38080.1	3.7e-06	39.37	hydroxyproline-rich glycoprotein family protein	[more]
AT2G22510.1	3.5e-04	38.66	hydroxyproline-rich glycoprotein family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044659	Protein PELPK-like	PANTHER	PTHR33088	MUCIN-2	coord: 1..104
None	No IPR available	PANTHER	PTHR33088:SF30	LEGUMINOSIN GROUP485 SECRETED PEPTIDE	coord: 1..104

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS012332	MS012332	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS012332.1-cds	MS012332.1-cds-scaffold63:38463..38774	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS012332.1	MS012332.1-protein	polypeptide