MS011721.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS011721.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin carboxyl-terminal hydrolase
Location	scaffold11: 169600 .. 170133 (+)
Sequence length	534
RNA-Seq Expression	MS011721.1
Synteny	MS011721.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCCTGGTGGGTTGCTTGGACCAATTCACTAGGCCCGAAAAACTTGGGTCCGACCAGAAATTCTTCTGCCAACAGTGTCAGGTGCGGCAGGAATCTCTCAAACAGATGTCCATAAGAAAGCTTCCGTTAGTTTCTTGTTTCCACATTAAAAGATTTGAGCATTCTTCGGTTCGGAAAATGTCGAGGAAGGTGGACCGATATTTGCAGTTTCCATTTTCTTTAGACATGGCACCGTATCTCTCATCTTCTATTTTGAGAAGTCGATTTGGGAATAGGATTTTCCCTTTTGATGGAGATGAACAAGATGCTTGCAACGAGTCATCGTCGGAGTTTGAGTTGTTTGCTGTCATCACTCACAAAGGTAAATTGGACGCCGGTCATTACGTGACCTACTTGCGGCTGAGTAATCAATGGTACAAGTGTGATGACGCTTGGATTACCCAAGTCAATGAAAATATTGTTAGGGCTGCACAAGGATACATGATGTTTTATGTGCAGAAGATGCTCTATTACAAAGCAAGTGAGAAACAGAGT

mRNA sequence

TCCCTGGTGGGTTGCTTGGACCAATTCACTAGGCCCGAAAAACTTGGGTCCGACCAGAAATTCTTCTGCCAACAGTGTCAGGTGCGGCAGGAATCTCTCAAACAGATGTCCATAAGAAAGCTTCCGTTAGTTTCTTGTTTCCACATTAAAAGATTTGAGCATTCTTCGGTTCGGAAAATGTCGAGGAAGGTGGACCGATATTTGCAGTTTCCATTTTCTTTAGACATGGCACCGTATCTCTCATCTTCTATTTTGAGAAGTCGATTTGGGAATAGGATTTTCCCTTTTGATGGAGATGAACAAGATGCTTGCAACGAGTCATCGTCGGAGTTTGAGTTGTTTGCTGTCATCACTCACAAAGGTAAATTGGACGCCGGTCATTACGTGACCTACTTGCGGCTGAGTAATCAATGGTACAAGTGTGATGACGCTTGGATTACCCAAGTCAATGAAAATATTGTTAGGGCTGCACAAGGATACATGATGTTTTATGTGCAGAAGATGCTCTATTACAAAGCAAGTGAGAAACAGAGT

Coding sequence (CDS)

TCCCTGGTGGGTTGCTTGGACCAATTCACTAGGCCCGAAAAACTTGGGTCCGACCAGAAATTCTTCTGCCAACAGTGTCAGGTGCGGCAGGAATCTCTCAAACAGATGTCCATAAGAAAGCTTCCGTTAGTTTCTTGTTTCCACATTAAAAGATTTGAGCATTCTTCGGTTCGGAAAATGTCGAGGAAGGTGGACCGATATTTGCAGTTTCCATTTTCTTTAGACATGGCACCGTATCTCTCATCTTCTATTTTGAGAAGTCGATTTGGGAATAGGATTTTCCCTTTTGATGGAGATGAACAAGATGCTTGCAACGAGTCATCGTCGGAGTTTGAGTTGTTTGCTGTCATCACTCACAAAGGTAAATTGGACGCCGGTCATTACGTGACCTACTTGCGGCTGAGTAATCAATGGTACAAGTGTGATGACGCTTGGATTACCCAAGTCAATGAAAATATTGTTAGGGCTGCACAAGGATACATGATGTTTTATGTGCAGAAGATGCTCTATTACAAAGCAAGTGAGAAACAGAGT

Protein sequence

SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS

Homology

[Close]

BLAST of MS011721.1 vs. NCBI nr
Match: XP_022143038.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Momordica charantia])

HSP 1 Score: 367.5 bits (942), Expect = 6.7e-98
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 236 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 295

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK
Sbjct: 296 SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 355

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS
Sbjct: 356 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 413

[Close]

BLAST of MS011721.1 vs. NCBI nr
Match: XP_038889878.1 (ubiquitin C-terminal hydrolase 22-like [Benincasa hispida])

HSP 1 Score: 364.8 bits (935), Expect = 4.3e-97
Identity = 176/178 (98.88%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 428 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 487

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 488 SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 547

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS
Sbjct: 548 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 605

[Close]

BLAST of MS011721.1 vs. NCBI nr
Match: XP_008457196.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo] >KAA0063520.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa] >TYJ97847.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa])

HSP 1 Score: 363.6 bits (932), Expect = 9.6e-97
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 423 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 482

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 483 SRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 542

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS
Sbjct: 543 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 600

[Close]

BLAST of MS011721.1 vs. NCBI nr
Match: XP_022999050.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita maxima])

HSP 1 Score: 362.5 bits (929), Expect = 2.1e-96
Identity = 174/178 (97.75%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 427 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 486

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 487 SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 546

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK+SEKQ+
Sbjct: 547 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKSSEKQN 604

[Close]

BLAST of MS011721.1 vs. NCBI nr
Match: XP_022946305.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita moschata] >XP_023547055.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita pepo subsp. pepo] >KAG7030363.1 Ubiquitin carboxyl-terminal hydrolase 22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362.5 bits (929), Expect = 2.1e-96
Identity = 174/178 (97.75%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 428 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 487

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 488 SRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 547

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQ+
Sbjct: 548 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQN 605

[Close]

BLAST of MS011721.1 vs. ExPASy Swiss-Prot
Match: Q9LEW0 (Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 2.6e-60
Identity = 111/181 (61.33%), Postives = 139/181 (76.80%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           +L GCLD FTR EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H+KRFEHS  RK 
Sbjct: 362 TLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSLTRKT 421

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGD-EQDACNES--SSEFELFAVI 120
           SRK+D YLQ+PF L+M+PYLSSSI+  RFGNRIF FDG+ E D+ + S  S+EFE+FAV+
Sbjct: 422 SRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIFAVV 481

Query: 121 THKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQ 179
           THKG L++GHYVTYLRL   WY+CDDAWI +V E +VR  + YM+FY Q+ +  KA ++ 
Sbjct: 482 THKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKAHKEL 541

[Close]

BLAST of MS011721.1 vs. ExPASy Swiss-Prot
Match: A6H8I0 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.4e-36
Identity = 75/173 (43.35%), Postives = 112/173 (64.74%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           +L  CL +FTRPE LGS  K  C  C   QES KQ+++++LP+V+CFH+KRFEHS+  K+
Sbjct: 342 TLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKRLPIVACFHLKRFEHSA--KL 401

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
            RK+  Y+ FP  LDM P+++SS      G    P D    D      +++ LFAV+ H+
Sbjct: 402 RRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPVDSLNND------NKYSLFAVVNHQ 461

Query: 121 GKLDAGHYVTYLRL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 173
           G L++GHY T++R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 462 GTLESGHYTTFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 506

[Close]

BLAST of MS011721.1 vs. ExPASy Swiss-Prot
Match: Q5DU02 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Mus musculus OX=10090 GN=Usp22 PE=2 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 3.4e-36
Identity = 75/173 (43.35%), Postives = 112/173 (64.74%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           +L  CL +FTRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+
Sbjct: 361 TLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KL 420

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
            RK+  Y+ FP  LDM P+++SS      G    P D    D      +++ LFAV+ H+
Sbjct: 421 RRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPLDSLNND------NKYSLFAVVNHQ 480

Query: 121 GKLDAGHYVTYLRL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 173
           G L++GHY +++R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 481 GTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 525

[Close]

BLAST of MS011721.1 vs. ExPASy Swiss-Prot
Match: Q6GNI6 (Ubiquitin carboxyl-terminal hydrolase 22-A OS=Xenopus laevis OX=8355 GN=usp22-a PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.8e-36
Identity = 75/173 (43.35%), Postives = 112/173 (64.74%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           +L  CL +FTRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+
Sbjct: 359 TLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KL 418

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
            RK+  Y+ FP  LDM P+++SS      G    P D    D      +++ LFAV+ H+
Sbjct: 419 RRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPSDSLHND------NKYSLFAVVNHQ 478

Query: 121 GKLDAGHYVTYLRL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 173
           G L++GHY +++R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 479 GTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 523

[Close]

BLAST of MS011721.1 vs. ExPASy Swiss-Prot
Match: Q9UPT9 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Homo sapiens OX=9606 GN=USP22 PE=1 SV=2)

HSP 1 Score: 152.1 bits (383), Expect = 5.8e-36
Identity = 75/173 (43.35%), Postives = 112/173 (64.74%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           +L  CL +FTRPE LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+
Sbjct: 361 TLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KL 420

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
            RK+  Y+ FP  LDM P+++SS      G    P D    D      +++ LFAV+ H+
Sbjct: 421 RRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNND------NKYSLFAVVNHQ 480

Query: 121 GKLDAGHYVTYLRL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 173
           G L++GHY +++R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 481 GTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 525

[Close]

BLAST of MS011721.1 vs. ExPASy TrEMBL
Match: A0A6J1CPR2 (Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC111013026 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.2e-98
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 236 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 295

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK
Sbjct: 296 SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 355

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS
Sbjct: 356 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 413

[Close]

BLAST of MS011721.1 vs. ExPASy TrEMBL
Match: A0A5D3BFE3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00590 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 4.7e-97
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 423 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 482

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 483 SRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 542

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS
Sbjct: 543 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 600

[Close]

BLAST of MS011721.1 vs. ExPASy TrEMBL
Match: A0A1S3C4I9 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo OX=3656 GN=LOC103496929 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 4.7e-97
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 423 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 482

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 483 SRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 542

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS
Sbjct: 543 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 600

[Close]

BLAST of MS011721.1 vs. ExPASy TrEMBL
Match: A0A6J1KE79 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493554 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.0e-96
Identity = 174/178 (97.75%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 427 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 486

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 487 SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 546

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK+SEKQ+
Sbjct: 547 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKSSEKQN 604

[Close]

BLAST of MS011721.1 vs. ExPASy TrEMBL
Match: A0A6J1G3H3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450426 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.0e-96
Identity = 174/178 (97.75%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM
Sbjct: 428 SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 487

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHK 120
           SRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHK
Sbjct: 488 SRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHK 547

Query: 121 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 179
           GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQ+
Sbjct: 548 GKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQN 605

[Close]

BLAST of MS011721.1 vs. TAIR 10
Match: AT5G10790.1 (ubiquitin-specific protease 22 )

HSP 1 Score: 233.0 bits (593), Expect = 1.8e-61
Identity = 111/181 (61.33%), Postives = 139/181 (76.80%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           +L GCLD FTR EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H+KRFEHS  RK 
Sbjct: 362 TLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSLTRKT 421

Query: 61  SRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGD-EQDACNES--SSEFELFAVI 120
           SRK+D YLQ+PF L+M+PYLSSSI+  RFGNRIF FDG+ E D+ + S  S+EFE+FAV+
Sbjct: 422 SRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIFAVV 481

Query: 121 THKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQ 179
           THKG L++GHYVTYLRL   WY+CDDAWI +V E +VR  + YM+FY Q+ +  KA ++ 
Sbjct: 482 THKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKAHKEL 541

[Close]

BLAST of MS011721.1 vs. TAIR 10
Match: AT4G10590.2 (ubiquitin-specific protease 10 )

HSP 1 Score: 104.4 bits (259), Expect = 9.8e-23
Identity = 55/167 (32.93%), Postives = 89/167 (53.29%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SL  CL+ F   E LG D  +FC  C+  +++ K++ + KLP +  FH+KRF +S  R +
Sbjct: 748 SLFSCLEAFLAEEPLGPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYS--RYL 807

Query: 61  SRKVDRYLQFP-FSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITH 120
             K+D ++ FP   LD++ Y+ +                      N+ S  +EL+AV  H
Sbjct: 808 KNKIDTFVNFPVHDLDLSKYVKNK---------------------NDQSYLYELYAVSNH 867

Query: 121 KGKLDAGHYVTYLRL--SNQWYKCDDAWITQVNENIVRAAQGYMMFY 165
            G L  GHY  Y +L   N+WY  DD+ ++ VNE+ ++ +  Y++FY
Sbjct: 868 YGGLGGGHYTAYAKLIDDNEWYHFDDSHVSSVNESEIKNSAAYVLFY 891

[Close]

BLAST of MS011721.1 vs. TAIR 10
Match: AT4G10590.1 (ubiquitin-specific protease 10 )

HSP 1 Score: 104.4 bits (259), Expect = 9.8e-23
Identity = 55/167 (32.93%), Postives = 89/167 (53.29%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SL  CL+ F   E LG D  +FC  C+  +++ K++ + KLP +  FH+KRF +S  R +
Sbjct: 748 SLFSCLEAFLAEEPLGPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYS--RYL 807

Query: 61  SRKVDRYLQFP-FSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITH 120
             K+D ++ FP   LD++ Y+ +                      N+ S  +EL+AV  H
Sbjct: 808 KNKIDTFVNFPVHDLDLSKYVKNK---------------------NDQSYLYELYAVSNH 867

Query: 121 KGKLDAGHYVTYLRL--SNQWYKCDDAWITQVNENIVRAAQGYMMFY 165
            G L  GHY  Y +L   N+WY  DD+ ++ VNE+ ++ +  Y++FY
Sbjct: 868 YGGLGGGHYTAYAKLIDDNEWYHFDDSHVSSVNESEIKNSAAYVLFY 891

[Close]

BLAST of MS011721.1 vs. TAIR 10
Match: AT4G10570.1 (ubiquitin-specific protease 9 )

HSP 1 Score: 103.6 bits (257), Expect = 1.7e-22
Identity = 56/167 (33.53%), Postives = 88/167 (52.69%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SL  CL+ F   E LG D  +FC  C+  +++ K++ + KLP +  FH+KRF +S  R +
Sbjct: 749 SLFSCLEAFLAEEPLGPDDMWFCPSCKEHRQANKKLDLWKLPDILVFHLKRFTYS--RYL 808

Query: 61  SRKVDRYLQFP-FSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITH 120
             K+D ++ FP   LD++ Y+ +                      N  S  +EL+AV  H
Sbjct: 809 KNKIDTFVNFPVHDLDLSKYVKNK---------------------NGQSYLYELYAVSNH 868

Query: 121 KGKLDAGHYVTYLRL--SNQWYKCDDAWITQVNENIVRAAQGYMMFY 165
            G L  GHY  Y +L   N+WY  DD+ ++ VNE+ +R +  Y++FY
Sbjct: 869 YGGLGGGHYTAYAKLIDDNKWYHFDDSHVSSVNESEIRNSAAYVLFY 892

[Close]

BLAST of MS011721.1 vs. TAIR 10
Match: AT2G40930.1 (ubiquitin-specific protease 5 )

HSP 1 Score: 100.5 bits (249), Expect = 1.4e-21
Identity = 53/170 (31.18%), Postives = 92/170 (54.12%), Query Frame = 0


Query: 1   SLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKM 60
           SL  CL+ F R E L  D+ +FC QC  R+++ K++ + +LP V   H+KRF +S  R M
Sbjct: 769 SLYACLEAFLREEPLVPDEMWFCPQCNERRQASKKLDLWRLPEVLVIHLKRFSYS--RSM 828

Query: 61  SRKVDRYLQFPF-SLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITH 120
             K++ ++ FP   LD+  Y+++  L                         +EL+A+  H
Sbjct: 829 KHKLETFVNFPIHDLDLTKYVANKNL--------------------SQPQLYELYALTNH 888

Query: 121 KGKLDAGHYVTYLRL--SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQK 168
            G + +GHY  +++L   ++WY  DD+ I+ +NE+ V++   Y++FY +K
Sbjct: 889 YGGMGSGHYTAHIKLLDDSRWYNFDDSHISHINEDDVKSGAAYVLFYRRK 916

The following BLAST results are available for this feature:

BLAST of MS011721.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143038.1	6.7e-98	100.00	ubiquitin carboxyl-terminal hydrolase 22-like [Momordica charantia]	[more]
XP_038889878.1	4.3e-97	98.88	ubiquitin C-terminal hydrolase 22-like [Benincasa hispida]	[more]
XP_008457196.1	9.6e-97	98.31	PREDICTED: ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo] >KAA0063...	[more]
XP_022999050.1	2.1e-96	97.75	ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita maxima]	[more]
XP_022946305.1	2.1e-96	97.75	ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita moschata] >XP_023547055...	[more]

BLAST of MS011721.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LEW0	2.6e-60	61.33	Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 ...	[more]
A6H8I0	3.4e-36	43.35	Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV...	[more]
Q5DU02	3.4e-36	43.35	Ubiquitin carboxyl-terminal hydrolase 22 OS=Mus musculus OX=10090 GN=Usp22 PE=2 ...	[more]
Q6GNI6	5.8e-36	43.35	Ubiquitin carboxyl-terminal hydrolase 22-A OS=Xenopus laevis OX=8355 GN=usp22-a ...	[more]
Q9UPT9	5.8e-36	43.35	Ubiquitin carboxyl-terminal hydrolase 22 OS=Homo sapiens OX=9606 GN=USP22 PE=1 S...	[more]

BLAST of MS011721.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPR2	3.2e-98	100.00	Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A5D3BFE3	4.7e-97	98.31	Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3C4I9	4.7e-97	98.31	Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo OX=3656 GN=LOC103496929 PE...	[more]
A0A6J1KE79	1.0e-96	97.75	Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149355...	[more]
A0A6J1G3H3	1.0e-96	97.75	Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450...	[more]

BLAST of MS011721.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10790.1	1.8e-61	61.33	ubiquitin-specific protease 22	[more]
AT4G10590.2	9.8e-23	32.93	ubiquitin-specific protease 10	[more]
AT4G10590.1	9.8e-23	32.93	ubiquitin-specific protease 10	[more]
AT4G10570.1	1.7e-22	33.53	ubiquitin-specific protease 9	[more]
AT2G40930.1	1.4e-21	31.18	ubiquitin-specific protease 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001394	Peptidase C19, ubiquitin carboxyl-terminal hydrolase	PFAM	PF00443	UCH	coord: 32..164 e-value: 4.0E-23 score: 82.3
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 1..168 e-value: 1.7E-46 score: 160.8
None	No IPR available	PANTHER	PTHR21646:SF66	UBIQUITINYL HYDROLASE 1	coord: 2..176
None	No IPR available	PANTHER	PTHR21646	UBIQUITIN CARBOXYL-TERMINAL HYDROLASE	coord: 2..176
IPR018200	Ubiquitin specific protease, conserved site	PROSITE	PS00973	USP_2	coord: 111..128
IPR028889	Ubiquitin specific protease domain	PROSITE	PS50235	USP_3	coord: 1..167 score: 33.565701
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..167

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS011721	MS011721	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS011721.1-cds	MS011721.1-cds-scaffold11:169600..170133	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS011721.1	MS011721.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
molecular_function	GO:0004843	thiol-dependent deubiquitinase