MS011230.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS011230.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	scaffold239: 273381 .. 274184 (-)
Sequence length	804
RNA-Seq Expression	MS011230.1
Synteny	MS011230.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGTTGGAAGGTAAAGTGGCCATAATTACCGGCGGCGCTAGTGGATTCGGGGAGAGCACCGCCAGATTATTCGTCCGGCACGGCGCCAAGGTGATCGTCGCCGACGTCCAAGATGAGCTCGGCCGGTCACTCTGTCAAGAACTCGGCCCGGAGAAAACCGTTTCGTATGTCCACTGCGATGTGACGAGCGATTCCGATGTGAAGAACGCCGTCGACGTCGCGGTGGCCAAGTACGGGAAGCTGGACATAATGTACAACAACGCCGGAATCACCGGCGAAATGGATCCGACGATTTTAGGAACAGAGGGGGAGAATTTCAAGAAGGTTTTCGAGGTGAATGTTTACGGCGGATTTTTAGGGGCGAAACACGCGGCTAGGGTTATGATTCCGAAGAGGAGAGGCGTGATTCTGTTCACGGCGAGTGTTGCGTCGGTGAATAGCGGAGAATCGCCGCACGCGTACGCGATGTCGAAGAACGCGGTGGTGGGATTGATGAGGAATTTGTGCGTCGAATTGGGGGAATTTGGGATTAGGGTTAATTCGGTATCTCCGGGCGCCATTGCGACGCCGTTACTGAGAAATGCGTTGGGATTCACGGAGGAGGCGTTGGAGGAGGTGATCCGGTCGACGGCGATCTTGAAAGGGGCGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTCTACTTGTGTAGCGACGAATCCAGGGTCATAAGCGGGCATAATCTTGTGGTGGACGGAGGGTACAGCACCGCCAACCGCTCCTTCTCCGCCGCTGTTATCAGAAAACTTTCATCCAACGGT

mRNA sequence

AGGTTGGAAGGTAAAGTGGCCATAATTACCGGCGGCGCTAGTGGATTCGGGGAGAGCACCGCCAGATTATTCGTCCGGCACGGCGCCAAGGTGATCGTCGCCGACGTCCAAGATGAGCTCGGCCGGTCACTCTGTCAAGAACTCGGCCCGGAGAAAACCGTTTCGTATGTCCACTGCGATGTGACGAGCGATTCCGATGTGAAGAACGCCGTCGACGTCGCGGTGGCCAAGTACGGGAAGCTGGACATAATGTACAACAACGCCGGAATCACCGGCGAAATGGATCCGACGATTTTAGGAACAGAGGGGGAGAATTTCAAGAAGGTTTTCGAGGTGAATGTTTACGGCGGATTTTTAGGGGCGAAACACGCGGCTAGGGTTATGATTCCGAAGAGGAGAGGCGTGATTCTGTTCACGGCGAGTGTTGCGTCGGTGAATAGCGGAGAATCGCCGCACGCGTACGCGATGTCGAAGAACGCGGTGGTGGGATTGATGAGGAATTTGTGCGTCGAATTGGGGGAATTTGGGATTAGGGTTAATTCGGTATCTCCGGGCGCCATTGCGACGCCGTTACTGAGAAATGCGTTGGGATTCACGGAGGAGGCGTTGGAGGAGGTGATCCGGTCGACGGCGATCTTGAAAGGGGCGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTCTACTTGTGTAGCGACGAATCCAGGGTCATAAGCGGGCATAATCTTGTGGTGGACGGAGGGTACAGCACCGCCAACCGCTCCTTCTCCGCCGCTGTTATCAGAAAACTTTCATCCAACGGT

Coding sequence (CDS)

AGGTTGGAAGGTAAAGTGGCCATAATTACCGGCGGCGCTAGTGGATTCGGGGAGAGCACCGCCAGATTATTCGTCCGGCACGGCGCCAAGGTGATCGTCGCCGACGTCCAAGATGAGCTCGGCCGGTCACTCTGTCAAGAACTCGGCCCGGAGAAAACCGTTTCGTATGTCCACTGCGATGTGACGAGCGATTCCGATGTGAAGAACGCCGTCGACGTCGCGGTGGCCAAGTACGGGAAGCTGGACATAATGTACAACAACGCCGGAATCACCGGCGAAATGGATCCGACGATTTTAGGAACAGAGGGGGAGAATTTCAAGAAGGTTTTCGAGGTGAATGTTTACGGCGGATTTTTAGGGGCGAAACACGCGGCTAGGGTTATGATTCCGAAGAGGAGAGGCGTGATTCTGTTCACGGCGAGTGTTGCGTCGGTGAATAGCGGAGAATCGCCGCACGCGTACGCGATGTCGAAGAACGCGGTGGTGGGATTGATGAGGAATTTGTGCGTCGAATTGGGGGAATTTGGGATTAGGGTTAATTCGGTATCTCCGGGCGCCATTGCGACGCCGTTACTGAGAAATGCGTTGGGATTCACGGAGGAGGCGTTGGAGGAGGTGATCCGGTCGACGGCGATCTTGAAAGGGGCGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTCTACTTGTGTAGCGACGAATCCAGGGTCATAAGCGGGCATAATCTTGTGGTGGACGGAGGGTACAGCACCGCCAACCGCTCCTTCTCCGCCGCTGTTATCAGAAAACTTTCATCCAACGGT

Protein sequence

RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCDVTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLGAKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVNSVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISGHNLVVDGGYSTANRSFSAAVIRKLSSNG

Homology

[Close]

BLAST of MS011230.1 vs. NCBI nr
Match: XP_022133043.1 (momilactone A synthase-like [Momordica charantia])

HSP 1 Score: 519.6 bits (1337), Expect = 1.6e-143
Identity = 267/268 (99.63%), Postives = 267/268 (99.63%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD
Sbjct: 41  RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 100

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG
Sbjct: 101 VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 160

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN
Sbjct: 161 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 220

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG
Sbjct: 221 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 280

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSSNG 269
           HNLVVDGGYSTAN SFSAAVIRKLSSNG
Sbjct: 281 HNLVVDGGYSTANLSFSAAVIRKLSSNG 308

[Close]

BLAST of MS011230.1 vs. NCBI nr
Match: XP_022979096.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 484.6 bits (1246), Expect = 5.6e-133
Identity = 245/266 (92.11%), Postives = 256/266 (96.24%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVAIITG ASGFG+STARLFV+HGA V+VADV DELGRSLCQELG EKTVSYVHCD
Sbjct: 9   RLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDELGRSLCQELGSEKTVSYVHCD 68

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVD AV +YGKLDIMYNNAGITGEMDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 69  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEVNVYGGFLG 128

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP+R GVILFT+SVASVNSGESPHAY MSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 129 AKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVELGEFGIRVN 188

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           S+SPGAIATPLLRNALGFTEEALEEV+RS+AILKG VPTAEDVAEAALYLCSDESRVISG
Sbjct: 189 SISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCSDESRVISG 248

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSS 267
           HNLVVDGGYSTANRSFSAAVIRKLSS
Sbjct: 249 HNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of MS011230.1 vs. NCBI nr
Match: KAG6604329.1 (hypothetical protein SDJN03_04938, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483.4 bits (1243), Expect = 1.3e-132
Identity = 244/266 (91.73%), Postives = 256/266 (96.24%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVAIITG ASGFG+STARLFV+HGA V+VADV D+LGRSLCQELG EKTVSYVHCD
Sbjct: 9   RLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEKTVSYVHCD 68

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVD AV +YGKLDIMYNNAGITGEMDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 69  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEVNVYGGFLG 128

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP+R GVILFT+SVASVNSGESPHAY MSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 129 AKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVELGEFGIRVN 188

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           S+SPGAIATPLLRNALGFTEEALEEV+RS+AILKG VPTAEDVAEAALYLCSDESRVISG
Sbjct: 189 SISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCSDESRVISG 248

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSS 267
           HNLVVDGGYSTANRSFSAAVIRKLSS
Sbjct: 249 HNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of MS011230.1 vs. NCBI nr
Match: XP_022925996.1 (secoisolariciresinol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 481.9 bits (1239), Expect = 3.7e-132
Identity = 243/267 (91.01%), Postives = 257/267 (96.25%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVAIITG ASGFG+STARLFV+HGA V+VADV D+LGRSLCQELG EKTVSY+HCD
Sbjct: 9   RLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEKTVSYLHCD 68

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVD AV +YGKLDIMYNNAGITGEMDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 69  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEVNVYGGFLG 128

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP+R G+ILFT+SVASVNSGESPHAY MSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 129 AKHAARVMIPRRSGLILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVELGEFGIRVN 188

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           S+SPGAIATPLLRNALGFTEEALEEVIRS+AILKG VPTAEDVAEAALYLCSDESRVISG
Sbjct: 189 SISPGAIATPLLRNALGFTEEALEEVIRSSAILKGVVPTAEDVAEAALYLCSDESRVISG 248

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSSN 268
           HNLVVDGGYSTANRSFSAAVIRKLSS+
Sbjct: 249 HNLVVDGGYSTANRSFSAAVIRKLSSS 275

[Close]

BLAST of MS011230.1 vs. NCBI nr
Match: XP_023543891.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.1 bits (1237), Expect = 6.2e-132
Identity = 242/266 (90.98%), Postives = 256/266 (96.24%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVAIITG ASGFG+STARLFV+HGA V+VADV D+LGRSLCQELG EKTVSYVHCD
Sbjct: 9   RLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEKTVSYVHCD 68

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKN+VD AV +YGKLDIMYNNAGITGEMDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 69  VTSDSDVKNSVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEVNVYGGFLG 128

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP+R GVILFT+SVASVNSGESPHAY MSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 129 AKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVELGEFGIRVN 188

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           S+SPGAIATPLLRNALGFTEEALEE++RS+AILKG VPTAEDVAEAALYLCSDESRVISG
Sbjct: 189 SISPGAIATPLLRNALGFTEEALEELVRSSAILKGVVPTAEDVAEAALYLCSDESRVISG 248

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSS 267
           HNLVVDGGYSTANRSFSAAVIRKLSS
Sbjct: 249 HNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of MS011230.1 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-76
Identity = 139/261 (53.26%), Postives = 186/261 (71.26%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL+ KVAIITGGA G GE+TA+LFVR+GAKV++AD+ D+ G+ +C  +G    +S+VHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VT D DV+N VD  +AK+GKLDIM+ N G+      +IL    E+FK+V ++NVYG FL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGES-PHAYAMSKNAVVGLMRNLCVELGEFGIRV 180
           AKHAARVMIP ++G I+FTAS++S  +GE   H Y  +K+AV+GL  +LC ELGE+GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 181 NSVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVIS 240
           N VSP  +A+PLL +  G     +EE+    A LKG +  AEDVA+A  YL  DES+ +S
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGDESKYVS 252

Query: 241 GHNLVVDGGYSTANRSFSAAV 261
           G NLV+DGGY+  N +F  A+
Sbjct: 253 GLNLVIDGGYTRTNPAFPTAL 273

[Close]

BLAST of MS011230.1 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 6.6e-76
Identity = 138/261 (52.87%), Postives = 185/261 (70.88%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL+ KVAIITGGA G GE+TA+LFVR+GAKV++AD+ D+ G+ +C  +G    +S+VHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VT D DV+N VD  +AK+GKLDIM+ N G+      +IL    E+FK+V ++NVYG FL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGES-PHAYAMSKNAVVGLMRNLCVELGEFGIRV 180
           AKHAARVMIP ++G I+FTAS++S  +GE   H Y  +K+AV+GL  +LC ELG+ GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 181 NSVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVIS 240
           N VSP  +A+PLL +  G     +EE+    A LKG +  AEDVA+A  YL  DES+ +S
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGDESKYVS 252

Query: 241 GHNLVVDGGYSTANRSFSAAV 261
           G NLV+DGGY+  N +F  A+
Sbjct: 253 GLNLVIDGGYTRTNPAFPTAL 273

[Close]

BLAST of MS011230.1 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.6e-74
Identity = 147/257 (57.20%), Postives = 187/257 (72.76%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           +L GKVA+ITGGASG G  TARLFV+HGA+V+VAD+QDELG SL  ELGP+ + SYVHCD
Sbjct: 14  KLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDAS-SYVHCD 73

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VT++ DV  AVD AVA++GKLD+M+NNAG++G     +     E+F++V  VN+ G FLG
Sbjct: 74  VTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFLG 133

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
            KHAARVM P RRG I+ TAS++S  SG + HAY  SK+A+VG   N   ELG  GIRVN
Sbjct: 134 TKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRVN 193

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGA-VPTAEDVAEAALYLCSDESRVIS 240
            VSP  +ATPL R A+G  +EA+E ++ ++A LKGA    A+D+A AAL+L SD+ R +S
Sbjct: 194 CVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYVS 253

Query: 241 GHNLVVDGGYSTANRSF 257
           G NL VDGG S  N SF
Sbjct: 254 GQNLRVDGGLSVVNSSF 269

[Close]

BLAST of MS011230.1 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 5.2e-73
Identity = 146/254 (57.48%), Postives = 181/254 (71.26%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RLEGKVA+ITGGASG GE+TA+LF +HGAKV +ADVQDELG S+ + +G   + +Y+HCD
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNS-TYIHCD 73

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VT++  VKNAVD  V+ YGKLDIM++NAGI+    P I+  E  +F++VF VNV G FL 
Sbjct: 74  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLC 133

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
            KHAARVMIP R G I+ TAS++S   G S HAY  SK+AV+GL RNL VELG+FGIRVN
Sbjct: 134 MKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVN 193

Query: 181 SVSPGAIATPLLRNALGF-TEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVIS 240
            +SP  + T L +   G   EE  E VI     LKG     EDVA AALYL SDE++ +S
Sbjct: 194 CLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKYVS 253

Query: 241 GHNLVVDGGYSTAN 254
           GHNL +DGG+S  N
Sbjct: 254 GHNLFIDGGFSVCN 266

[Close]

BLAST of MS011230.1 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.6e-69
Identity = 141/261 (54.02%), Postives = 176/261 (67.43%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RLEGKVA++TGGASG GES ARLF+ HGAK+ + DVQDELG+ + Q LG +    Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VT + DV+ AVD    KYG +DIM NNAGITG+    I   +   FKKVF++NV G FLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
            KHAAR+MIPK +G I+  ASV+SV +G  PH Y  +K+AVVGL +++  ELG  GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 181 SVSPGAIATPLLRNAL---GFTEEALE---EVIRSTAILKGAVPTAEDVAEAALYLCSDE 240
            VSP A+ T L    L      E+AL      +RS A LKG      DVAEA LYL ++E
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATEE 241

Query: 241 SRVISGHNLVVDGGYSTANRS 256
           S+ +SG NLV+DGG+S AN +
Sbjct: 242 SKYVSGLNLVIDGGFSIANHT 262

[Close]

BLAST of MS011230.1 vs. ExPASy TrEMBL
Match: A0A6J1BXW7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111005735 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 7.6e-144
Identity = 267/268 (99.63%), Postives = 267/268 (99.63%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD
Sbjct: 41  RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 100

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG
Sbjct: 101 VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 160

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN
Sbjct: 161 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 220

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG
Sbjct: 221 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 280

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSSNG 269
           HNLVVDGGYSTAN SFSAAVIRKLSSNG
Sbjct: 281 HNLVVDGGYSTANLSFSAAVIRKLSSNG 308

[Close]

BLAST of MS011230.1 vs. ExPASy TrEMBL
Match: A0A6J1IPT5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111478837 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.7e-133
Identity = 245/266 (92.11%), Postives = 256/266 (96.24%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVAIITG ASGFG+STARLFV+HGA V+VADV DELGRSLCQELG EKTVSYVHCD
Sbjct: 9   RLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDELGRSLCQELGSEKTVSYVHCD 68

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVD AV +YGKLDIMYNNAGITGEMDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 69  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEVNVYGGFLG 128

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP+R GVILFT+SVASVNSGESPHAY MSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 129 AKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVELGEFGIRVN 188

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           S+SPGAIATPLLRNALGFTEEALEEV+RS+AILKG VPTAEDVAEAALYLCSDESRVISG
Sbjct: 189 SISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCSDESRVISG 248

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSS 267
           HNLVVDGGYSTANRSFSAAVIRKLSS
Sbjct: 249 HNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of MS011230.1 vs. ExPASy TrEMBL
Match: A0A6J1EDN5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433246 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.8e-132
Identity = 243/267 (91.01%), Postives = 257/267 (96.25%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVAIITG ASGFG+STARLFV+HGA V+VADV D+LGRSLCQELG EKTVSY+HCD
Sbjct: 9   RLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEKTVSYLHCD 68

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVD AV +YGKLDIMYNNAGITGEMDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 69  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEVNVYGGFLG 128

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP+R G+ILFT+SVASVNSGESPHAY MSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 129 AKHAARVMIPRRSGLILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVELGEFGIRVN 188

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           S+SPGAIATPLLRNALGFTEEALEEVIRS+AILKG VPTAEDVAEAALYLCSDESRVISG
Sbjct: 189 SISPGAIATPLLRNALGFTEEALEEVIRSSAILKGVVPTAEDVAEAALYLCSDESRVISG 248

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSSN 268
           HNLVVDGGYSTANRSFSAAVIRKLSS+
Sbjct: 249 HNLVVDGGYSTANRSFSAAVIRKLSSS 275

[Close]

BLAST of MS011230.1 vs. ExPASy TrEMBL
Match: A0A5A7SIU8 (Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001660 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.0e-127
Identity = 232/266 (87.22%), Postives = 253/266 (95.11%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVA+ITG ASGFG+STARLFV+HGA+V++ADVQD L + LC+ELG E++VSY+HCD
Sbjct: 10  RLNGKVALITGAASGFGKSTARLFVQHGARVVLADVQDNLAQPLCKELGSEESVSYIHCD 69

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVD AV +YGKLDIMYNNAGITG+MDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 70  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGQMDPTILGTDGENFKKVFEVNVYGGFLG 129

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP R GVILFT+SVASVNSGESPHAYAMSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 130 AKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIRVN 189

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           SVSPGAIATPLLRNALGFT++ LEEV+RS+AILKG V TAEDVAEAALYLCSDESRVISG
Sbjct: 190 SVSPGAIATPLLRNALGFTKKELEEVVRSSAILKGVVATAEDVAEAALYLCSDESRVISG 249

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSS 267
           HNLVVDGGYSTANRSFSAAVIRK+SS
Sbjct: 250 HNLVVDGGYSTANRSFSAAVIRKVSS 275

[Close]

BLAST of MS011230.1 vs. ExPASy TrEMBL
Match: A0A1S3CHD5 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103500923 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.0e-127
Identity = 232/266 (87.22%), Postives = 253/266 (95.11%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL GKVA+ITG ASGFG+STARLFV+HGA+V++ADVQD L + LC+ELG E++VSY+HCD
Sbjct: 10  RLNGKVALITGAASGFGKSTARLFVQHGARVVLADVQDNLAQPLCKELGSEESVSYIHCD 69

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VTSDSDVKNAVD AV +YGKLDIMYNNAGITG+MDPTILGT+GENFKKVFEVNVYGGFLG
Sbjct: 70  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGQMDPTILGTDGENFKKVFEVNVYGGFLG 129

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
           AKHAARVMIP R GVILFT+SVASVNSGESPHAYAMSK+AVVGLMRNLCVELGEFGIRVN
Sbjct: 130 AKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIRVN 189

Query: 181 SVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVISG 240
           SVSPGAIATPLLRNALGFT++ LEEV+RS+AILKG V TAEDVAEAALYLCSDESRVISG
Sbjct: 190 SVSPGAIATPLLRNALGFTKKELEEVVRSSAILKGVVATAEDVAEAALYLCSDESRVISG 249

Query: 241 HNLVVDGGYSTANRSFSAAVIRKLSS 267
           HNLVVDGGYSTANRSFSAAVIRK+SS
Sbjct: 250 HNLVVDGGYSTANRSFSAAVIRKVSS 275

[Close]

BLAST of MS011230.1 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 3.4e-67
Identity = 138/267 (51.69%), Postives = 179/267 (67.04%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKT---VSYV 60
           RLEGKVAIITGGA G G++T  LF RHGA V++ADV +  G SL + L   KT   V+++
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 61  HCDVTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEM--DPTILGTEGENFKKVFEVNVY 120
            CDV+ ++DV+N V+V VA+YG+LDI++NNAG+ G+     +IL  + + F  V  VNV 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 121 GGFLGAKHAARVMIPKR-RGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGE 180
           G  LG KH AR MI +  +G I+ TASVA V  G  PHAY  SK+A+VGL +N   ELG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 181 FGIRVNSVSPGAIATPLLRNALGFTE---------EALEEVIRSTAILKGAVPTAEDVAE 240
           +GIRVN +SP  +AT +L NA   T          E +EE +RS A LKG    A D+AE
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAE 270

Query: 241 AALYLCSDESRVISGHNLVVDGGYSTA 253
           AALYL SDES+ ++GHNLVVDGG +TA
Sbjct: 271 AALYLASDESKYVNGHNLVVDGGVTTA 297

[Close]

BLAST of MS011230.1 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 4.5e-64
Identity = 126/256 (49.22%), Postives = 174/256 (67.97%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           +LEGKVA+ITGGASG G++TA  F+ HGAKVI+AD+Q ++GR   QELGP  + +Y  CD
Sbjct: 77  KLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGP--SCAYFPCD 136

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VT +SD+ NAVD AV+ + KLDIMYNNAGI  +  P+I+  +   F KV   NV G   G
Sbjct: 137 VTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRGVMAG 196

Query: 121 AKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRVN 180
            KHAARVMIP+  G I+   SV  +  G + H Y++SK+AV+G++R+   EL +  IRVN
Sbjct: 197 IKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRIRVN 256

Query: 181 SVSPGAIATPLLRNAL-----GFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDES 240
            +SP AI T  + + +     G  +  L ++++ST +L G V    DVA AA+YL SD+S
Sbjct: 257 CISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLASDDS 316

Query: 241 RVISGHNLVVDGGYST 252
           + ++GHNLVVDGG++T
Sbjct: 317 KYVNGHNLVVDGGFTT 330

[Close]

BLAST of MS011230.1 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.3e-63
Identity = 124/254 (48.82%), Postives = 171/254 (67.32%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           +LEGKVA+ITGGASG G++TA  F+RHGA+V++AD+  E G    +ELG E    +V CD
Sbjct: 40  KLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSE--AEFVRCD 99

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDP-TILGTEGENFKKVFEVNVYGGFL 120
           VT ++D+  AV++ V +YGKLD+MYNNAGI G M P +I   +   F++V  +NV+G   
Sbjct: 100 VTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVVS 159

Query: 121 GAKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRV 180
           G KHAA+ MIP R G IL T+SVA V  G +PH+Y +SK    G++++   EL E G+R+
Sbjct: 160 GIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVRI 219

Query: 181 NSVSPGAIATPLLRNAL-----GFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDE 240
           N +SPG +ATPL  + L       +EE L E ++    LKGA     DVA+AALYL S++
Sbjct: 220 NCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALYLASND 279

Query: 241 SRVISGHNLVVDGG 249
            + ++GHNLVVDGG
Sbjct: 280 GKYVTGHNLVVDGG 291

[Close]

BLAST of MS011230.1 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 4.2e-62
Identity = 128/265 (48.30%), Postives = 168/265 (63.40%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQEL---GPEKTVSYV 60
           RL GKVA+ITGGA+G GES  RLF +HGAKV + D+QD+LG  +C+ L     ++T  ++
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 61  HCDVTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGG 120
           H DV  + D+ NAVD AV  +G LDI+ NNAG+ G   P I       F+  F+VNV G 
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 121 FLGAKHAARVMIPKRRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGI 180
           FL  KHAARVMIP+++G I+   SV  V  G  PH+Y  SK+AV+GL R++  ELG+ GI
Sbjct: 137 FLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGI 196

Query: 181 RVNSVSPGAIATPLLRNALGFTEEALEEVI------RSTAILKGAVPTAEDVAEAALYLC 240
           RVN VSP A+AT L    L   E   +  +       + A LKG   T +DVA A L+L 
Sbjct: 197 RVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVANAVLFLA 256

Query: 241 SDESRVISGHNLVVDGGYSTANRSF 257
           SD+SR ISG NL++DGG++  N SF
Sbjct: 257 SDDSRYISGDNLMIDGGFTCTNHSF 281

[Close]

BLAST of MS011230.1 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 226.9 bits (577), Expect = 2.0e-59
Identity = 124/251 (49.40%), Postives = 165/251 (65.74%), Query Frame = 0


Query: 1   RLEGKVAIITGGASGFGESTARLFVRHGAKVIVADVQDELGRSLCQELGPEKTVSYVHCD 60
           RL+GK+AIITGGASG G    RLF  HGAKV++ D Q+ELG+++   +G +K  S+  CD
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDK-ASFYRCD 64

Query: 61  VTSDSDVKNAVDVAVAKYGKLDIMYNNAGITGEMDPTILGTEGENFKKVFEVNVYGGFLG 120
           VT++ +V+NAV   V KYGKLD++++NAG+  E   + L    E F +   VNV G    
Sbjct: 65  VTNEKEVENAVKFTVEKYGKLDVLFSNAGVM-EQPGSFLDLNLEQFDRTMAVNVRGAAAF 124

Query: 121 AKHAARVMIPK-RRGVILFTASVASVNSGESPHAYAMSKNAVVGLMRNLCVELGEFGIRV 180
            KHAAR M+ K  RG I+ T SVAS   G  PHAY  SK+A++GL+++ C  LG++GIRV
Sbjct: 125 IKHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRV 184

Query: 181 NSVSPGAIATPLLRNALGFTEEALEEVIRSTAILKGAVPTAEDVAEAALYLCSDESRVIS 240
           N V+P A+AT  + +    T   +EE   +T ILKG V  A  VAEAAL+L SD+S  +S
Sbjct: 185 NGVAPYAVAT-AINSRDEETVRMVEEYSAATGILKGVVLKARHVAEAALFLASDDSAYVS 244

Query: 241 GHNLVVDGGYS 251
           G NL VDGGYS
Sbjct: 245 GQNLAVDGGYS 252

The following BLAST results are available for this feature:

BLAST of MS011230.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133043.1	1.6e-143	99.63	momilactone A synthase-like [Momordica charantia]	[more]
XP_022979096.1	5.6e-133	92.11	secoisolariciresinol dehydrogenase-like [Cucurbita maxima]	[more]
KAG6604329.1	1.3e-132	91.73	hypothetical protein SDJN03_04938, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022925996.1	3.7e-132	91.01	secoisolariciresinol dehydrogenase-like [Cucurbita moschata]	[more]
XP_023543891.1	6.2e-132	90.98	secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS011230.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL8	1.3e-76	53.26	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	6.6e-76	52.87	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q7FAE1	1.6e-74	57.20	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
Q94KL7	5.2e-73	57.48	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
F1SWA0	1.6e-69	54.02	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of MS011230.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BXW7	7.6e-144	99.63	momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111005735 PE=4 ...	[more]
A0A6J1IPT5	2.7e-133	92.11	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111478...	[more]
A0A6J1EDN5	1.8e-132	91.01	secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A5A7SIU8	5.0e-127	87.22	Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CHD5	5.0e-127	87.22	secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103500923 ...	[more]

BLAST of MS011230.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	3.4e-67	51.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	4.5e-64	49.22	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	1.3e-63	48.82	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	4.2e-62	48.30	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47130.1	2.0e-59	49.40	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 6..23 score: 45.29 coord: 128..144 score: 31.47 coord: 154..173 score: 37.34 coord: 175..192 score: 48.36 coord: 213..233 score: 30.41 coord: 79..90 score: 57.66
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 154..173 score: 33.2 coord: 79..90 score: 50.98 coord: 134..142 score: 43.34
None	No IPR available	GENE3D	3.40.50.720		coord: 1..265 e-value: 2.1E-82 score: 278.2
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 11..250 e-value: 2.3E-67 score: 227.0
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 2..253 e-value: 7.8E-25 score: 85.3
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 8..201 e-value: 7.2E-21 score: 72.4
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 1..261
None	No IPR available	PANTHER	PTHR43180:SF45	SECOISOLARICIRESINOL DEHYDROGENASE-RELATED	coord: 1..261
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..250

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS011230	MS011230	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS011230.1-cds	MS011230.1-cds-scaffold239:273381..274184	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS011230.1	MS011230.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity