MS010268.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS010268.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Chlororespiratory reduction 42
Location	scaffold391: 1268107 .. 1268617 (+)
Sequence length	399
RNA-Seq Expression	MS010268.1
Synteny	MS010268.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTCCGCTTTCTGCTGCTACCGGAATTCCACGCCCAAGCATTAGCATCATCAATCCAATCTCTTCATTCAACTTCTCCAACTGCTTAAGCACCGGTATTCAACCGCGAAGCTTCGTCATCAAGTGTGAATCAAGTGCATCGGCAGCGGACGAGACGCCGCCGGCAAAGAGCCGGAAACTCGAGATCGGCTCCCCCGTAATCGTAGTCGAGGCTCCCAAAATGATAAAGACCGCAGCCTCAGTTCCATGCCTGCGGGTCAATTCCGGCATAATCAACCCCGGCGACGTTGGCAGGTGAGTGGCCGATCGGCCGTCCTCCTCTCTCTTCGGCATTTCTTCGAACATATTATGTGCGTCGCCTGTAACCAATTCCTGTTGCGTTGCCCTAACATGTATGCTCGAACAGAATCGTGTCGAGAAAACCTAAAGACGTGTGGGCCGTGCGGCTTAAAGTGGGGACTTACCTCATCGATGGAAGGTATTTTAAACCATTAGAGCTCGATCAG

mRNA sequence

ATGGCTGCTCCGCTTTCTGCTGCTACCGGAATTCCACGCCCAAGCATTAGCATCATCAATCCAATCTCTTCATTCAACTTCTCCAACTGCTTAAGCACCGGTATTCAACCGCGAAGCTTCGTCATCAAGTGTGAATCAAGTGCATCGGCAGCGGACGAGACGCCGCCGGCAAAGAGCCGGAAACTCGAGATCGGCTCCCCCGTAATCGTAGTCGAGGCTCCCAAAATGATAAAGACCGCAGCCTCAGTTCCATGCCTGCGGGTCAATTCCGGCATAATCAACCCCGGCGACGTTGGCAGAATCGTGTCGAGAAAACCTAAAGACGTGTGGGCCGTGCGGCTTAAAGTGGGGACTTACCTCATCGATGGAAGGTATTTTAAACCATTAGAGCTCGATCAG

Coding sequence (CDS)

ATGGCTGCTCCGCTTTCTGCTGCTACCGGAATTCCACGCCCAAGCATTAGCATCATCAATCCAATCTCTTCATTCAACTTCTCCAACTGCTTAAGCACCGGTATTCAACCGCGAAGCTTCGTCATCAAGTGTGAATCAAGTGCATCGGCAGCGGACGAGACGCCGCCGGCAAAGAGCCGGAAACTCGAGATCGGCTCCCCCGTAATCGTAGTCGAGGCTCCCAAAATGATAAAGACCGCAGCCTCAGTTCCATGCCTGCGGGTCAATTCCGGCATAATCAACCCCGGCGACGTTGGCAGAATCGTGTCGAGAAAACCTAAAGACGTGTGGGCCGTGCGGCTTAAAGTGGGGACTTACCTCATCGATGGAAGGTATTTTAAACCATTAGAGCTCGATCAG

Protein sequence

MAAPLSAATGIPRPSISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAKSRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTYLIDGRYFKPLELDQ

Homology

[Close]

BLAST of MS010268.1 vs. NCBI nr
Match: XP_022153826.1 (uncharacterized protein LOC111021250 [Momordica charantia])

HSP 1 Score: 233.4 bits (594), Expect = 1.1e-57
Identity = 121/124 (97.58%), Postives = 121/124 (97.58%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPSISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAKSR 60
           MAA LSAAT IPRPSISIIN ISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAKSR
Sbjct: 1   MAASLSAATAIPRPSISIINSISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAKSR 60

Query: 61  KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTYL 120
           KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTYL
Sbjct: 61  KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTYL 120

Query: 121 IDGR 125
           IDGR
Sbjct: 121 IDGR 124

[Close]

BLAST of MS010268.1 vs. NCBI nr
Match: XP_022985824.1 (uncharacterized protein LOC111483753 [Cucurbita maxima])

HSP 1 Score: 220.7 bits (561), Expect = 7.5e-54
Identity = 118/135 (87.41%), Postives = 121/135 (89.63%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPS--ISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAK 60
           MA  LS+AT IPRPS  I I+N ISSFN SN LST  QPRSFV KCESSASAAD TPPAK
Sbjct: 1   MATSLSSATAIPRPSIGIGIVNSISSFNISNSLSTRSQPRSFV-KCESSASAADPTPPAK 60

Query: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGT 120
           SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVW VRLKVGT
Sbjct: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWGVRLKVGT 120

Query: 121 YLIDGRYFKPLELDQ 134
           YLIDGRYF+PLELDQ
Sbjct: 121 YLIDGRYFRPLELDQ 134

[Close]

BLAST of MS010268.1 vs. NCBI nr
Match: XP_023513105.1 (uncharacterized protein LOC111777646 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216.1 bits (549), Expect = 1.9e-52
Identity = 115/135 (85.19%), Postives = 121/135 (89.63%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPS--ISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAK 60
           MA  LS+AT IPRPS  I I++ ISSFN SN LST  QPRS ++KCESSASAAD TPPAK
Sbjct: 1   MATSLSSATAIPRPSIGIGIVSSISSFNVSNSLSTRSQPRS-LVKCESSASAADPTPPAK 60

Query: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGT 120
           SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVW VRLKVGT
Sbjct: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWGVRLKVGT 120

Query: 121 YLIDGRYFKPLELDQ 134
           YLIDGRYF+PLELDQ
Sbjct: 121 YLIDGRYFRPLELDQ 134

[Close]

BLAST of MS010268.1 vs. NCBI nr
Match: XP_022944245.1 (uncharacterized protein LOC111448751 [Cucurbita moschata])

HSP 1 Score: 213.0 bits (541), Expect = 1.6e-51
Identity = 114/135 (84.44%), Postives = 120/135 (88.89%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPS--ISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAK 60
           MA  LS+AT IPRPS  I I++ ISSFN SN LST  QPRS ++KCESSASAAD TPPAK
Sbjct: 1   MATSLSSATAIPRPSIGIGIVSSISSFNVSNSLSTRSQPRS-LVKCESSASAADPTPPAK 60

Query: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGT 120
           S KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVW VRLKVGT
Sbjct: 61  SWKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWGVRLKVGT 120

Query: 121 YLIDGRYFKPLELDQ 134
           YLIDGRYF+PLELDQ
Sbjct: 121 YLIDGRYFRPLELDQ 134

[Close]

BLAST of MS010268.1 vs. NCBI nr
Match: KAG6570328.1 (hypothetical protein SDJN03_29243, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010222.1 hypothetical protein SDJN02_27014, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 213.0 bits (541), Expect = 1.6e-51
Identity = 114/135 (84.44%), Postives = 120/135 (88.89%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPSISI--INPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAK 60
           MA  LS+AT IPRPSI I  ++ ISSFN SN LST  QPRS ++KCESSASAAD TPPAK
Sbjct: 1   MATSLSSATAIPRPSIGIGTVSSISSFNVSNSLSTRSQPRS-LVKCESSASAADPTPPAK 60

Query: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGT 120
           S KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVW VRLKVGT
Sbjct: 61  SWKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWGVRLKVGT 120

Query: 121 YLIDGRYFKPLELDQ 134
           YLIDGRYF+PLELDQ
Sbjct: 121 YLIDGRYFRPLELDQ 134

[Close]

BLAST of MS010268.1 vs. ExPASy TrEMBL
Match: A0A6J1DLV6 (uncharacterized protein LOC111021250 OS=Momordica charantia OX=3673 GN=LOC111021250 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 5.4e-58
Identity = 121/124 (97.58%), Postives = 121/124 (97.58%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPSISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAKSR 60
           MAA LSAAT IPRPSISIIN ISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAKSR
Sbjct: 1   MAASLSAATAIPRPSISIINSISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAKSR 60

Query: 61  KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTYL 120
           KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTYL
Sbjct: 61  KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTYL 120

Query: 121 IDGR 125
           IDGR
Sbjct: 121 IDGR 124

[Close]

BLAST of MS010268.1 vs. ExPASy TrEMBL
Match: A0A6J1JEC4 (uncharacterized protein LOC111483753 OS=Cucurbita maxima OX=3661 GN=LOC111483753 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.6e-54
Identity = 118/135 (87.41%), Postives = 121/135 (89.63%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPS--ISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAK 60
           MA  LS+AT IPRPS  I I+N ISSFN SN LST  QPRSFV KCESSASAAD TPPAK
Sbjct: 1   MATSLSSATAIPRPSIGIGIVNSISSFNISNSLSTRSQPRSFV-KCESSASAADPTPPAK 60

Query: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGT 120
           SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVW VRLKVGT
Sbjct: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWGVRLKVGT 120

Query: 121 YLIDGRYFKPLELDQ 134
           YLIDGRYF+PLELDQ
Sbjct: 121 YLIDGRYFRPLELDQ 134

[Close]

BLAST of MS010268.1 vs. ExPASy TrEMBL
Match: A0A6J1FYJ7 (uncharacterized protein LOC111448751 OS=Cucurbita moschata OX=3662 GN=LOC111448751 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 7.6e-52
Identity = 114/135 (84.44%), Postives = 120/135 (88.89%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPS--ISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAADETPPAK 60
           MA  LS+AT IPRPS  I I++ ISSFN SN LST  QPRS ++KCESSASAAD TPPAK
Sbjct: 1   MATSLSSATAIPRPSIGIGIVSSISSFNVSNSLSTRSQPRS-LVKCESSASAADPTPPAK 60

Query: 61  SRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGT 120
           S KLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVW VRLKVGT
Sbjct: 61  SWKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWGVRLKVGT 120

Query: 121 YLIDGRYFKPLELDQ 134
           YLIDGRYF+PLELDQ
Sbjct: 121 YLIDGRYFRPLELDQ 134

[Close]

BLAST of MS010268.1 vs. ExPASy TrEMBL
Match: A0A5A7T9E1 (Chlororespiratory reduction 42 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00650 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.1e-49
Identity = 110/134 (82.09%), Postives = 117/134 (87.31%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPSISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAAD-ETPPAKS 60
           MAA  S  T I RPSI  +  ISSFN SN LST  QPRS ++KCESSASAAD +TPPAKS
Sbjct: 1   MAASFSPTTPISRPSIRTLTSISSFNVSNNLSTRSQPRS-IVKCESSASAADGQTPPAKS 60

Query: 61  RKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTY 120
           +KLEIGSPVIV+EAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTY
Sbjct: 61  QKLEIGSPVIVIEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTY 120

Query: 121 LIDGRYFKPLELDQ 134
           LIDGRYF+ LELDQ
Sbjct: 121 LIDGRYFRALELDQ 133

[Close]

BLAST of MS010268.1 vs. ExPASy TrEMBL
Match: A0A1S3BMX2 (uncharacterized protein LOC103491596 OS=Cucumis melo OX=3656 GN=LOC103491596 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.1e-49
Identity = 110/134 (82.09%), Postives = 117/134 (87.31%), Query Frame = 0


Query: 1   MAAPLSAATGIPRPSISIINPISSFNFSNCLSTGIQPRSFVIKCESSASAAD-ETPPAKS 60
           MAA  S  T I RPSI  +  ISSFN SN LST  QPRS ++KCESSASAAD +TPPAKS
Sbjct: 1   MAASFSPTTPISRPSIRTLTSISSFNVSNNLSTRSQPRS-IVKCESSASAADGQTPPAKS 60

Query: 61  RKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTY 120
           +KLEIGSPVIV+EAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTY
Sbjct: 61  QKLEIGSPVIVIEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVWAVRLKVGTY 120

Query: 121 LIDGRYFKPLELDQ 134
           LIDGRYF+ LELDQ
Sbjct: 121 LIDGRYFRALELDQ 133

[Close]

BLAST of MS010268.1 vs. TAIR 10
Match: AT5G20935.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3148 (InterPro:IPR021495); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 132.9 bits (333), Expect = 1.9e-31
Identity = 61/83 (73.49%), Postives = 75/83 (90.36%), Query Frame = 0


Query: 51  ADETPPAKSRKLEIGSPVIVVEAPKMIKTAASVPCLRVNSGIINPGDVGRIVSRKPKDVW 110
           A+E P  KS KL++GSP+I+VEAPK+IKTAAS+PCLR NSG++ PGDVGRIVSRKPKD+W
Sbjct: 23  ANEAPRPKS-KLQVGSPIIIVEAPKVIKTAASMPCLRANSGLVKPGDVGRIVSRKPKDLW 82

Query: 111 AVRLKVGTYLIDGRYFKPLELDQ 134
           AVRL +GTYL+DG+YFK LELD+
Sbjct: 83  AVRLSIGTYLLDGKYFKALELDE 104

The following BLAST results are available for this feature:

BLAST of MS010268.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153826.1	1.1e-57	97.58	uncharacterized protein LOC111021250 [Momordica charantia]	[more]
XP_022985824.1	7.5e-54	87.41	uncharacterized protein LOC111483753 [Cucurbita maxima]	[more]
XP_023513105.1	1.9e-52	85.19	uncharacterized protein LOC111777646 [Cucurbita pepo subsp. pepo]	[more]
XP_022944245.1	1.6e-51	84.44	uncharacterized protein LOC111448751 [Cucurbita moschata]	[more]
KAG6570328.1	1.6e-51	84.44	hypothetical protein SDJN03_29243, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MS010268.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS010268.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLV6	5.4e-58	97.58	uncharacterized protein LOC111021250 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1JEC4	3.6e-54	87.41	uncharacterized protein LOC111483753 OS=Cucurbita maxima OX=3661 GN=LOC111483753...	[more]
A0A6J1FYJ7	7.6e-52	84.44	uncharacterized protein LOC111448751 OS=Cucurbita moschata OX=3662 GN=LOC1114487...	[more]
A0A5A7T9E1	2.1e-49	82.09	Chlororespiratory reduction 42 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3BMX2	2.1e-49	82.09	uncharacterized protein LOC103491596 OS=Cucumis melo OX=3656 GN=LOC103491596 PE=...	[more]

BLAST of MS010268.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20935.1	1.9e-31	73.49	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3148...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021495	Protein of unknown function DUF3148	PFAM	PF11347	DUF3148	coord: 64..126 e-value: 7.0E-25 score: 86.6
IPR021495	Protein of unknown function DUF3148	PANTHER	PTHR36799	FAMILY NOT NAMED	coord: 24..132
None	No IPR available	PANTHER	PTHR36799:SF2	DUF3148 FAMILY PROTEIN	coord: 24..132

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS010268	MS010268	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS010268.1-cds	MS010268.1-cds-scaffold391:1268107..1268405	CDS
MS010268.1-cds	MS010268.1-cds-scaffold391:1268518..1268617	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS010268.1	MS010268.1-protein	polypeptide