MS010234.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS010234.1 vs. NCBI nr
Match: KAG6570369.1 (Delta(12)-oleate desaturase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 713.8 bits (1841), Expect = 8.0e-202
Identity = 322/375 (85.87%), Postives = 351/375 (93.60%), Query Frame = 0

Query: 1   MGVGKRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYL 60
           MGVG +M+KAV  R PHAKPPFTLSQIKKA+PPHCF RSLLRSFS L+SDL FVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSA 120
           AIA  PL+PHP  YIAWP+YW+FQGC LTGVWVIAHECGHHAFSDYQL+DDIVGL FHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPLYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLG 180
           LLVPYFSWKYSHRRHHSNTGSLERDEVFVP+ KS+IPW++KYFNNPPGRLISLL TLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGAL 240
           WPLYL FNVSGRPYDRFACHYDP+GPIFTKREW+Q+YISD GI+ M FV+YRI VAKGAL
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQVYISDTGILGMCFVVYRIGVAKGAL 240

Query: 241 WVMRIYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYG+PL+IVNGFLVLITYLQHTHPALPHY+S+EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDSAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDD 360
           NITDTHVVHHLFSTMPHY+A+EATEAVKP+LGEYYRLDRTPV KA+WREA+ECVYV PD+
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 DPNSVRGVVWYRNKI 376
           D NS +GVVWYRNK+
Sbjct: 361 DANSDKGVVWYRNKL 375

BLAST of MS010234.1 vs. NCBI nr
Match: XP_023512996.1 (delta(12)-oleate desaturase [Cucurbita pepo subsp. pepo] >XP_023522472.1 delta(12)-oleate desaturase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712.6 bits (1838), Expect = 1.8e-201
Identity = 323/375 (86.13%), Postives = 350/375 (93.33%), Query Frame = 0

Query: 1   MGVGKRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYL 60
           MGVG +M+KAV  R PHAKPPFTLSQIKKA+PPHCF RSLLRSFS L+SDL FVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSA 120
           AIA  PL+PHP  YIAWP YW+FQGC LTGVWVIAHECGHHAFSDYQL+DDIVGL FHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPFYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLG 180
           LLVPYFSWKYSHRRHHSNTGSLERDEVFVP+ KS+IPW++KYFNNPPGRLISLL TLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGAL 240
           WPLYL FNVSGRPYDRFACHYDP+GPIFTKREW+QIYISD GI+ M  VLYRI+VAKGAL
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQIYISDTGILGMCLVLYRISVAKGAL 240

Query: 241 WVMRIYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYG+PL+IVNGFLVLITYLQHTHPALPHY+S+EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDSAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDD 360
           NITDTHVVHHLFSTMPHY+A+EATEAVKP+LGEYYRLDRTPV KA+WREA+ECVYV PD+
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 DPNSVRGVVWYRNKI 376
           D NS +GVVWYRNK+
Sbjct: 361 DDNSDKGVVWYRNKL 375

BLAST of MS010234.1 vs. NCBI nr
Match: Q84UB9.1 (RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2 [Trichosanthes kirilowii] >AAO37752.1 delta-12 oleate desaturase [Trichosanthes kirilowii])

HSP 1 Score: 710.7 bits (1833), Expect = 6.8e-201
Identity = 322/369 (87.26%), Postives = 346/369 (93.77%), Query Frame = 0

Query: 7   MDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFP 66
           M+K V  R PHAKPPFTLSQIKK VPPHCF RSLLRSFSYLLSDL FVSLFFYLAIA FP
Sbjct: 1   MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYFP 60

Query: 67  LLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYF 126
           LLPHP  YIAWP+YWIFQGC+LTGVWVIAHECGHHAFSDYQL+DDIVGL FHSALLVPYF
Sbjct: 61  LLPHPFPYIAWPLYWIFQGCSLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 120

Query: 127 SWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLT 186
           SWKYSHRRHHSNTGSLERDEVFVP+ KS+IPWY+KYFNNPPGRLISL+ TLTLGWPLYL 
Sbjct: 121 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGWPLYLA 180

Query: 187 FNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIY 246
           FN+SGRPYDRFACHYDPY PIFTKREW+Q+YISDAGI+ MAF+LYRI V KG+ WVMRIY
Sbjct: 181 FNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFWVMRIY 240

Query: 247 GVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 306
           G+PLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH
Sbjct: 241 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 300

Query: 307 VVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNSVR 366
           VVHHLFSTMPHY+A+EATEAVKP+LGEYYRLDRTPV KA+WREA+ECVYVEPD+D +S +
Sbjct: 301 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDEDADSDK 360

Query: 367 GVVWYRNKI 376
           GVVWYR K+
Sbjct: 361 GVVWYRKKL 369

BLAST of MS010234.1 vs. NCBI nr
Match: XP_022943671.1 (delta(12)-oleate desaturase [Cucurbita moschata])

HSP 1 Score: 710.3 bits (1832), Expect = 8.8e-201
Identity = 321/375 (85.60%), Postives = 350/375 (93.33%), Query Frame = 0

Query: 1   MGVGKRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYL 60
           MGVG +M+KAV  R PHAKPPFTLSQIKKA+PPHCF RSLLRSFS L+SDL FVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSA 120
           AIA  PL+PHP  YIAWP+YW+FQGC LTGVWVIAHECGHHAFSDYQL+DDIVGL FHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPLYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLG 180
           LLVPYFSWKYSHRRHHSNT SLERDEVFVP+ KS+IPW++KYFNNPPGRLISLL TLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTVSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGAL 240
           WPLYL FNVSGRPYDRFACHYDP+GPIFTKREW+QIYISD GI+ M FVLYRI VAKGAL
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQIYISDTGILGMCFVLYRIGVAKGAL 240

Query: 241 WVMRIYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYG+PL+IVNGFLVLITYLQHTHPALPHY+++EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDAAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDD 360
           NITDTHVVHHLFSTMPHY+A+EATEAVKP+LGEYYRLDRTPV KA+WREA+ECVYV PD+
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 DPNSVRGVVWYRNKI 376
           D NS +GV+WYRNK+
Sbjct: 361 DANSDKGVIWYRNKL 375

BLAST of MS010234.1 vs. NCBI nr
Match: XP_022995104.1 (delta(12)-oleate desaturase [Cucurbita maxima])

HSP 1 Score: 679.5 bits (1752), Expect = 1.7e-191
Identity = 304/365 (83.29%), Postives = 335/365 (91.78%), Query Frame = 0

Query: 8   DKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFPL 67
           +K    R PHAKPPFTL+QIKKA+PPHCF RSLLRSF+ LLSDL  VSLFFYLAIA FPL
Sbjct: 3   EKGTHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTCLLSDLASVSLFFYLAIAYFPL 62

Query: 68  LPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYFS 127
           +P+P  YIAWP+YW+FQGCTLTG+WVIAHECGHHAFSDYQL+DD VG   HSALLVPYFS
Sbjct: 63  IPYPFPYIAWPLYWVFQGCTLTGIWVIAHECGHHAFSDYQLIDDTVGFFLHSALLVPYFS 122

Query: 128 WKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLTF 187
           WKYSHRRHHSNTGSLERDEVFVP+ KS++PWY KYFNNPPGRLISL+ATLTLGWPLYL F
Sbjct: 123 WKYSHRRHHSNTGSLERDEVFVPKPKSQVPWYSKYFNNPPGRLISLIATLTLGWPLYLAF 182

Query: 188 NVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIYG 247
           NVSGRPYDR ACHYDPY PIFTKREW+Q+YISDAGI+ MAFVLYRIA+AKGA WVMRIYG
Sbjct: 183 NVSGRPYDRLACHYDPYAPIFTKREWIQVYISDAGILGMAFVLYRIAMAKGAFWVMRIYG 242

Query: 248 VPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHV 307
           +PL+IVNGFLVLIT+LQHTHPALPHY+SSEW+WLRGALATVDRDYGVLNRVFHNITDTHV
Sbjct: 243 IPLLIVNGFLVLITFLQHTHPALPHYDSSEWNWLRGALATVDRDYGVLNRVFHNITDTHV 302

Query: 308 VHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNSVRG 367
            HHLFSTMPHY+A+EATEA+KP+LGEYYR DRTPV KA+WRE +ECVYVE D+D NS +G
Sbjct: 303 AHHLFSTMPHYHAKEATEAIKPVLGEYYRFDRTPVLKAIWRETKECVYVEADEDANSEKG 362

Query: 368 VVWYR 373
           VVWYR
Sbjct: 363 VVWYR 367

BLAST of MS010234.1 vs. ExPASy Swiss-Prot
Match: Q84UB9 (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAD2 PE=2 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 8.9e-204
Identity = 322/369 (87.26%), Postives = 346/369 (93.77%), Query Frame = 0

Query: 7   MDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFP 66
           M+K V  R PHAKPPFTLSQIKK VPPHCF RSLLRSFSYLLSDL FVSLFFYLAIA FP
Sbjct: 1   MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYFP 60

Query: 67  LLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYF 126
           LLPHP  YIAWP+YWIFQGC+LTGVWVIAHECGHHAFSDYQL+DDIVGL FHSALLVPYF
Sbjct: 61  LLPHPFPYIAWPLYWIFQGCSLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 120

Query: 127 SWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLT 186
           SWKYSHRRHHSNTGSLERDEVFVP+ KS+IPWY+KYFNNPPGRLISL+ TLTLGWPLYL 
Sbjct: 121 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGWPLYLA 180

Query: 187 FNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIY 246
           FN+SGRPYDRFACHYDPY PIFTKREW+Q+YISDAGI+ MAF+LYRI V KG+ WVMRIY
Sbjct: 181 FNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFWVMRIY 240

Query: 247 GVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 306
           G+PLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH
Sbjct: 241 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 300

Query: 307 VVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNSVR 366
           VVHHLFSTMPHY+A+EATEAVKP+LGEYYRLDRTPV KA+WREA+ECVYVEPD+D +S +
Sbjct: 301 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDEDADSDK 360

Query: 367 GVVWYRNKI 376
           GVVWYR K+
Sbjct: 361 GVVWYRKKL 369

BLAST of MS010234.1 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 3.6e-173
Identity = 271/370 (73.24%), Postives = 314/370 (84.86%), Query Frame = 0

Query: 5   KRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIAC 64
           K+++  V  R PH+KPPFTL Q+KKA+PPHCF RS+LRSFSY++ DL    +F+Y+A   
Sbjct: 14  KKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNY 73

Query: 65  FPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVP 124
           F LLP PL Y+AWPIYW  QGC LTGVWVIAHECGHHAFSDYQL+DDIVGL+ HS LLVP
Sbjct: 74  FHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVP 133

Query: 125 YFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLY 184
           YFSWK+SHRRHHSNT SLERDEVFVP+ KS I W+ KY NNPPGRL +L  TLTLGWPLY
Sbjct: 134 YFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLY 193

Query: 185 LTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMR 244
           L FNVSGRPYDRFACHYDPYGPI+T RE  +IYISDAG++++ F LYR+A AKG  WV+ 
Sbjct: 194 LAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVIC 253

Query: 245 IYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITD 304
           +YGVPL+IVN FLV+ITYLQHTHP++PHY+SSEWDWLRGALATVDRDYG+LN+VFHNITD
Sbjct: 254 VYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITD 313

Query: 305 THVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNS 364
           THV HHLFSTMPHY+A EAT+A+KPILGEYY+ D TP +KAMWREA+EC+YVE DD   S
Sbjct: 314 THVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEADDGDES 373

Query: 365 VRGVVWYRNK 375
            +GV WY  K
Sbjct: 374 -KGVYWYNKK 382

BLAST of MS010234.1 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 607.1 bits (1564), Expect = 1.4e-172
Identity = 272/374 (72.73%), Postives = 314/374 (83.96%), Query Frame = 0

Query: 2   GVGKRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLA 61
           G G    +    R P AKPPFTLS+IKKA+PPHCF RSLL+SFSY+L DL  V++F+Y+A
Sbjct: 15  GEGDEKSQKPVQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYVLYDLTLVAIFYYVA 74

Query: 62  IACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSAL 121
                 LP PL Y AWP+YW  QGC LTGVWVIAHECGHHAFSDYQ VDD VGL+ HSAL
Sbjct: 75  TTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQWVDDCVGLVLHSAL 134

Query: 122 LVPYFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGW 181
           LVPYFSWKYSHRRHHSNTGSLERDEVFVP+ KSK+PW+ KY NNPPGR+++L+ TLTLGW
Sbjct: 135 LVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNPPGRVMTLIVTLTLGW 194

Query: 182 PLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALW 241
           PLYL  NVSG PYDRFACH+DPYGPI+T RE LQIYISD GI++  + LY+IA A+G  W
Sbjct: 195 PLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAATYTLYKIAAARGLAW 254

Query: 242 VMRIYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHN 301
           ++ +YGVPL+IVN FLV ITYLQHTHPALPHY+SSEWDWLRGALAT DRDYG+LN+VFHN
Sbjct: 255 LVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALATADRDYGILNKVFHN 314

Query: 302 ITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDD 361
           ITDTHV HHLFSTMPHY+A EAT+A+KPILG+YY+ D TPV+KAMWREAREC+YVEPDD 
Sbjct: 315 ITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAMWREARECLYVEPDDG 374

Query: 362 PNSVRGVVWYRNKI 376
            NS +GV WY+  +
Sbjct: 375 ANS-KGVFWYKKNL 387

BLAST of MS010234.1 vs. ExPASy Swiss-Prot
Match: P48631 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX=3847 GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 9.3e-169
Identity = 277/384 (72.14%), Postives = 313/384 (81.51%), Query Frame = 0

Query: 1   MGVGKRMD-------KAVD--ARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDL 60
           MG G R D         VD   R P  KP F+LSQIKKA+PPHCF RS+LRSFSY++ DL
Sbjct: 1   MGAGGRTDVPPANRKSEVDPLKRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  CFVSLFFYLAIACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDD 120
                 +Y+A   F LLP PL +    IYW  QGC LTGVWVIAHECGHHAFSDYQL+DD
Sbjct: 61  TIAFCLYYVATHYFHLLPGPLSFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 IVGLIFHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLI 180
           IVGLI HSALLVPYFSWKYSHRRHHSNTGSLERDEVFVP+ KS I WY KY NNPPGR++
Sbjct: 121 IVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVL 180

Query: 181 SLLATLTLGWPLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLY 240
           +L  TLTLGWPLYL  NVSGRPYDRFACHYDPYGPI++ RE LQIYISDAG++++ + L+
Sbjct: 181 TLAVTLTLGWPLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLF 240

Query: 241 RIAVAKGALWVMRIYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRD 300
           R+A+AKG  WV+ +YGVPL++VNGFLVLIT+LQHTHPALPHY SSEWDWLRGALATVDRD
Sbjct: 241 RLAMAKGLAWVVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRD 300

Query: 301 YGVLNRVFHNITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREAR 360
           YG+LN+VFHNITDTHV HHLFSTMPHY+A EAT+A+KPILGEYYR D TP  KAMWREAR
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREAR 360

Query: 361 ECVYVEPDDDPNSVRGVVWYRNKI 376
           EC+YVEPD    S +GV WY NK+
Sbjct: 361 ECIYVEPDQSTES-KGVFWYNNKL 383

BLAST of MS010234.1 vs. ExPASy Swiss-Prot
Match: Q41131 (Oleate hydroxylase FAH12 OS=Ricinus communis OX=3988 GN=FAH12 PE=1 SV=2)

HSP 1 Score: 592.0 bits (1525), Expect = 4.6e-168
Identity = 263/361 (72.85%), Postives = 306/361 (84.76%), Query Frame = 0

Query: 14  RAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFPLLPHPLE 73
           RAPH KPPFTL  +K+A+PPHCF+RS +RSFSY+  D+C   LF+ +A   FP +  PL 
Sbjct: 27  RAPHTKPPFTLGDLKRAIPPHCFERSFVRSFSYVAYDVCLSFLFYSIATNFFPYISSPLS 86

Query: 74  YIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYFSWKYSHR 133
           Y+AW +YW+FQGC LTG+WVI HECGHHAFS+YQL DDIVGLI HSALLVPYFSWKYSHR
Sbjct: 87  YVAWLVYWLFQGCILTGLWVIGHECGHHAFSEYQLADDIVGLIVHSALLVPYFSWKYSHR 146

Query: 134 RHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLTFNVSGRP 193
           RHHSN GSLERDEVFVP+ KSKI WY KY NNPPGR+++L ATL LGWPLYL FNVSGRP
Sbjct: 147 RHHSNIGSLERDEVFVPKSKSKISWYSKYLNNPPGRVLTLAATLLLGWPLYLAFNVSGRP 206

Query: 194 YDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIYGVPLVIV 253
           YDRFACHYDPYGPIF++RE LQIYI+D GI +  FVLY+  +AKG  WVMRIYGVPL+IV
Sbjct: 207 YDRFACHYDPYGPIFSERERLQIYIADLGIFATTFVLYQATMAKGLAWVMRIYGVPLLIV 266

Query: 254 NGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVVHHLFS 313
           N FLV+ITYLQHTHPA+P Y SSEWDWLRGA+ TVDRDYGVLN+VFHNI DTHV HHLF+
Sbjct: 267 NCFLVMITYLQHTHPAIPRYGSSEWDWLRGAMVTVDRDYGVLNKVFHNIADTHVAHHLFA 326

Query: 314 TMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNSVRGVVWYRN 373
           T+PHY+A EAT+A+KPI+GEYYR D TP +KA+WREA+EC++VEPD+     +GV WYRN
Sbjct: 327 TVPHYHAMEATKAIKPIMGEYYRYDGTPFYKALWREAKECLFVEPDEGA-PTQGVFWYRN 386

Query: 374 K 375
           K
Sbjct: 387 K 386

BLAST of MS010234.1 vs. ExPASy TrEMBL
Match: A0A6J1FSC4 (delta(12)-oleate desaturase OS=Cucurbita moschata OX=3662 GN=LOC111448353 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.3e-201
Identity = 321/375 (85.60%), Postives = 350/375 (93.33%), Query Frame = 0

Query: 1   MGVGKRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYL 60
           MGVG +M+KAV  R PHAKPPFTLSQIKKA+PPHCF RSLLRSFS L+SDL FVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSA 120
           AIA  PL+PHP  YIAWP+YW+FQGC LTGVWVIAHECGHHAFSDYQL+DDIVGL FHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPLYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLG 180
           LLVPYFSWKYSHRRHHSNT SLERDEVFVP+ KS+IPW++KYFNNPPGRLISLL TLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTVSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGAL 240
           WPLYL FNVSGRPYDRFACHYDP+GPIFTKREW+QIYISD GI+ M FVLYRI VAKGAL
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQIYISDTGILGMCFVLYRIGVAKGAL 240

Query: 241 WVMRIYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYG+PL+IVNGFLVLITYLQHTHPALPHY+++EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDAAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDD 360
           NITDTHVVHHLFSTMPHY+A+EATEAVKP+LGEYYRLDRTPV KA+WREA+ECVYV PD+
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 DPNSVRGVVWYRNKI 376
           D NS +GV+WYRNK+
Sbjct: 361 DANSDKGVIWYRNKL 375

BLAST of MS010234.1 vs. ExPASy TrEMBL
Match: A0A6J1K4R6 (delta(12)-oleate desaturase OS=Cucurbita maxima OX=3661 GN=LOC111490747 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 8.1e-192
Identity = 304/365 (83.29%), Postives = 335/365 (91.78%), Query Frame = 0

Query: 8   DKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFPL 67
           +K    R PHAKPPFTL+QIKKA+PPHCF RSLLRSF+ LLSDL  VSLFFYLAIA FPL
Sbjct: 3   EKGTHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTCLLSDLASVSLFFYLAIAYFPL 62

Query: 68  LPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYFS 127
           +P+P  YIAWP+YW+FQGCTLTG+WVIAHECGHHAFSDYQL+DD VG   HSALLVPYFS
Sbjct: 63  IPYPFPYIAWPLYWVFQGCTLTGIWVIAHECGHHAFSDYQLIDDTVGFFLHSALLVPYFS 122

Query: 128 WKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLTF 187
           WKYSHRRHHSNTGSLERDEVFVP+ KS++PWY KYFNNPPGRLISL+ATLTLGWPLYL F
Sbjct: 123 WKYSHRRHHSNTGSLERDEVFVPKPKSQVPWYSKYFNNPPGRLISLIATLTLGWPLYLAF 182

Query: 188 NVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIYG 247
           NVSGRPYDR ACHYDPY PIFTKREW+Q+YISDAGI+ MAFVLYRIA+AKGA WVMRIYG
Sbjct: 183 NVSGRPYDRLACHYDPYAPIFTKREWIQVYISDAGILGMAFVLYRIAMAKGAFWVMRIYG 242

Query: 248 VPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHV 307
           +PL+IVNGFLVLIT+LQHTHPALPHY+SSEW+WLRGALATVDRDYGVLNRVFHNITDTHV
Sbjct: 243 IPLLIVNGFLVLITFLQHTHPALPHYDSSEWNWLRGALATVDRDYGVLNRVFHNITDTHV 302

Query: 308 VHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNSVRG 367
            HHLFSTMPHY+A+EATEA+KP+LGEYYR DRTPV KA+WRE +ECVYVE D+D NS +G
Sbjct: 303 AHHLFSTMPHYHAKEATEAIKPVLGEYYRFDRTPVLKAIWRETKECVYVEADEDANSEKG 362

Query: 368 VVWYR 373
           VVWYR
Sbjct: 363 VVWYR 367

BLAST of MS010234.1 vs. ExPASy TrEMBL
Match: A0A6J1H0J3 (delta(12)-oleate desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111459302 PE=4 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 2.4e-191
Identity = 306/368 (83.15%), Postives = 338/368 (91.85%), Query Frame = 0

Query: 8   DKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFPL 67
           +K V  R PHAKPPFTL+QIKKA+PPHCF RSLLRSF+ LLSDL  VSLF YLAIA F L
Sbjct: 3   EKGVHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTCLLSDLASVSLFSYLAIAHFRL 62

Query: 68  LPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYFS 127
           +P+P  YIAWP+YW+FQGCTLTG+WVIAHECGHHAFSDYQL+DDIVG   HSALLVPYFS
Sbjct: 63  IPYPFPYIAWPLYWVFQGCTLTGIWVIAHECGHHAFSDYQLIDDIVGFFLHSALLVPYFS 122

Query: 128 WKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLTF 187
           WKYSHRRHHSNTGSLERDEVFVP+ KS+IPWY KYFNNPPGRLISL+ATLTLGWPLYL F
Sbjct: 123 WKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYSKYFNNPPGRLISLIATLTLGWPLYLAF 182

Query: 188 NVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIYG 247
           NVSGRPYDR+ACHYDPY PIFTKREW+Q+YISDAGI+ MAFVLYRIA+AKGA WVMRIYG
Sbjct: 183 NVSGRPYDRWACHYDPYAPIFTKREWIQVYISDAGILGMAFVLYRIAMAKGAFWVMRIYG 242

Query: 248 VPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHV 307
           +PL+IVNGFLVLIT+LQHTHPALPHY+SSEW+WLRGALATVDRDYGVLNRVFHNITDTHV
Sbjct: 243 IPLLIVNGFLVLITFLQHTHPALPHYDSSEWNWLRGALATVDRDYGVLNRVFHNITDTHV 302

Query: 308 VHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNSVRG 367
            HHLFSTMPHY+A+EATEA+K +LGEYYRLDRTPV KA+WREA+ECVYVE D+D NS +G
Sbjct: 303 AHHLFSTMPHYHAKEATEAIKAVLGEYYRLDRTPVLKAIWREAKECVYVEADEDANSEKG 362

Query: 368 VVWYRNKI 376
           VVWYR  +
Sbjct: 363 VVWYRKNL 370

BLAST of MS010234.1 vs. ExPASy TrEMBL
Match: W9QMT8 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Morus notabilis OX=981085 GN=L484_018419 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 7.1e-180
Identity = 296/386 (76.68%), Postives = 328/386 (84.97%), Query Frame = 0

Query: 1   MGVGKRM----------DKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSD 60
           MG G RM           K+   R PH  PPFTLSQIKKA+PPHCF RSL+RSFSY+  D
Sbjct: 1   MGAGGRMPVLSSNKLEGHKSQVERVPHTTPPFTLSQIKKAIPPHCFKRSLVRSFSYVGHD 60

Query: 61  LCFVSLFFYLAIACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVD 120
           L F +LF+Y+A + F LLPHPL Y+AWP+YWI QGC  TG+WVIAHECGHHAFSD+Q VD
Sbjct: 61  LFFAALFYYIATSYFHLLPHPLPYLAWPLYWIVQGCVCTGIWVIAHECGHHAFSDHQWVD 120

Query: 121 DIVGLIFHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRL 180
           D VGLI HSALLVPYFSWKYSHRRHHSNTGSLERDEVFVP+ KSKI WY KY NNPPGRL
Sbjct: 121 DTVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKISWYSKYLNNPPGRL 180

Query: 181 ISLLATLTLGWPLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVL 240
           +SLL TLTLGWPLYL FNVSGRPYDRFACHYDPYGPI++ RE LQI++SDAGI    FVL
Sbjct: 181 LSLLVTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFVSDAGIFVTTFVL 240

Query: 241 YRIAVAKGALWVMRIYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDR 300
           Y++A AKG  W++ +YGVPL+IVNGFLVLITYLQHTHPALPHY+SSEWDWLRGALATVDR
Sbjct: 241 YQLAAAKGLAWIVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDR 300

Query: 301 DYGVLNRVFHNITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREA 360
           DYGVLNRVFHNI DTHV HHLFSTMPHYNA EAT+AVKP+LGEYYR DRTP+FKAMWREA
Sbjct: 301 DYGVLNRVFHNINDTHVAHHLFSTMPHYNAMEATKAVKPLLGEYYRFDRTPIFKAMWREA 360

Query: 361 RECVYVEPDDD-PNSVRGVVWYRNKI 376
           +EC+YVEPDDD PN  +GV WYRNK+
Sbjct: 361 KECLYVEPDDDAPN--KGVFWYRNKL 384

BLAST of MS010234.1 vs. ExPASy TrEMBL
Match: A0A1Q3B5J9 (FA_desaturase domain-containing protein/DUF3474 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_06585 PE=3 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.7e-178
Identity = 287/361 (79.50%), Postives = 323/361 (89.47%), Query Frame = 0

Query: 14  RAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFPLLPHPLE 73
           RAP AKPPFTLSQ+KKA+PP CF RSLLRSFSY++ DLCFVSLF+Y+A + F LLP+PL 
Sbjct: 23  RAPSAKPPFTLSQVKKAIPPRCFQRSLLRSFSYVVFDLCFVSLFYYIATSYFHLLPYPLT 82

Query: 74  YIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYFSWKYSHR 133
           Y+AWP+YWIFQGC  TGVWVIAHECGHHAFSDYQ VDD VGLI HSALLVPYFSWKYSHR
Sbjct: 83  YLAWPLYWIFQGCVCTGVWVIAHECGHHAFSDYQWVDDTVGLILHSALLVPYFSWKYSHR 142

Query: 134 RHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLTFNVSGRP 193
           RHHSNTGSLERDEVFVP+ KS+IPW+ KY NNPPGR +SLL TLTLGWPLYL FNVSGRP
Sbjct: 143 RHHSNTGSLERDEVFVPKPKSQIPWFCKYLNNPPGRALSLLITLTLGWPLYLAFNVSGRP 202

Query: 194 YDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIYGVPLVIV 253
           Y+RFACHYDPYGPI++ RE LQI++SDAGI +   VLYR+AVAKG  W++ +YGVPL+IV
Sbjct: 203 YNRFACHYDPYGPIYSDRERLQIFLSDAGIFATTSVLYRLAVAKGLAWLVCLYGVPLLIV 262

Query: 254 NGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVVHHLFS 313
           NGFLVLITYLQHTHPALPHY+SSEW+WLRGALAT DRDYGVLN+VFHNITDTHV HHLFS
Sbjct: 263 NGFLVLITYLQHTHPALPHYDSSEWNWLRGALATADRDYGVLNKVFHNITDTHVAHHLFS 322

Query: 314 TMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNSVRGVVWYRN 373
           TMPHYNA EATE +KPILGEYY+ D TP++KA+WREAREC+YVEPD+D  S +GV WYRN
Sbjct: 323 TMPHYNAMEATEVIKPILGEYYQSDSTPIYKALWREARECLYVEPDEDA-SKKGVFWYRN 382

Query: 374 K 375
           K
Sbjct: 383 K 382

BLAST of MS010234.1 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 565.1 bits (1455), Expect = 4.3e-161
Identity = 256/371 (69.00%), Postives = 301/371 (81.13%), Query Frame = 0

Query: 5   KRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIAC 64
           K+ +     R P  KPPF++  +KKA+PPHCF RS+ RSFSYL+SD+   S F+Y+A   
Sbjct: 14  KKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDIIIASCFYYVATNY 73

Query: 65  FPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVP 124
           F LLP PL Y+AWP+YW  QGC LTG+WVIAHECGHHAFSDYQ +DD VGLIFHS LLVP
Sbjct: 74  FSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDDTVGLIFHSFLLVP 133

Query: 125 YFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLY 184
           YFSWKYSHRRHHSNTGSLERDEVFVP+ KS I WY KY NNP GR++ L     LGWPLY
Sbjct: 134 YFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIMMLTVQFVLGWPLY 193

Query: 185 LTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMR 244
           L FNVSGRPYD FACH+ P  PI+  RE LQIY+SDAGI+++ F LYR A A+G   ++ 
Sbjct: 194 LAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYRYAAAQGMASMIC 253

Query: 245 IYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITD 304
           +YGVPL+IVN FLVLITYLQHTHP+LPHY+SSEWDWLRGALATVDRDYG+LN+VFHNITD
Sbjct: 254 LYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNITD 313

Query: 305 THVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNS 364
           THV HHLFSTMPHYNA EAT+A+KPILG+YY+ D TP + AM+REA+EC+YVEPD + + 
Sbjct: 314 THVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPDREGDK 373

Query: 365 VRGVVWYRNKI 376
            +GV WY NK+
Sbjct: 374 -KGVYWYNNKL 383

BLAST of MS010234.1 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 565.1 bits (1455), Expect = 4.3e-161
Identity = 256/371 (69.00%), Postives = 301/371 (81.13%), Query Frame = 0

Query: 5   KRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIAC 64
           K+ +     R P  KPPF++  +KKA+PPHCF RS+ RSFSYL+SD+   S F+Y+A   
Sbjct: 14  KKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDIIIASCFYYVATNY 73

Query: 65  FPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVP 124
           F LLP PL Y+AWP+YW  QGC LTG+WVIAHECGHHAFSDYQ +DD VGLIFHS LLVP
Sbjct: 74  FSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDDTVGLIFHSFLLVP 133

Query: 125 YFSWKYSHRRHHSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLY 184
           YFSWKYSHRRHHSNTGSLERDEVFVP+ KS I WY KY NNP GR++ L     LGWPLY
Sbjct: 134 YFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIMMLTVQFVLGWPLY 193

Query: 185 LTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMR 244
           L FNVSGRPYD FACH+ P  PI+  RE LQIY+SDAGI+++ F LYR A A+G   ++ 
Sbjct: 194 LAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYRYAAAQGMASMIC 253

Query: 245 IYGVPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITD 304
           +YGVPL+IVN FLVLITYLQHTHP+LPHY+SSEWDWLRGALATVDRDYG+LN+VFHNITD
Sbjct: 254 LYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNITD 313

Query: 305 THVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRTPVFKAMWREARECVYVEPDDDPNS 364
           THV HHLFSTMPHYNA EAT+A+KPILG+YY+ D TP + AM+REA+EC+YVEPD + + 
Sbjct: 314 THVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPDREGDK 373

Query: 365 VRGVVWYRNKI 376
            +GV WY NK+
Sbjct: 374 -KGVYWYNNKL 383

BLAST of MS010234.1 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 233.8 bits (595), Expect = 2.3e-61
Identity = 134/345 (38.84%), Postives = 189/345 (54.78%), Query Frame = 0

Query: 2   GVGKRMDKAVDARAPHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLA 61
           G G R  K  +   P A+PPF +  I+ A+P HC+ +S LRS SY++ D+  V+    LA
Sbjct: 16  GAGDR--KKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAA---LA 75

Query: 62  IACFPLLPHPLEYIAWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSAL 121
           IA      +   +  WP+YW  QG     ++V+ H+CGH +FSD  L++ +VG I HS +
Sbjct: 76  IAAV----YVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFI 135

Query: 122 LVPYFSWKYSHRRHHSNTGSLERDEVFVP---RHKSKIPWYIKYFNNPPGRLISLLATL- 181
           LVPY  W+ SHR HH N G +E DE +VP   R   K+P           R++     L 
Sbjct: 136 LVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLP--------HSTRMLRYTVPLP 195

Query: 182 TLGWPLYLTFNVSGRPYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAK 241
            L +PLYL +   G+       H++PY  +F   E   I  S      M   L  ++   
Sbjct: 196 MLAYPLYLCYRSPGKE----GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVF 255

Query: 242 GALWVMRIYGVPLVIVNGFLVLITYLQH--THPALPHYESSEWDWLRGALATVDRDYGVL 301
           G L V+++YGVP +I   +L  +TYL H      LP Y   EW +LRG L T+DRDYG+ 
Sbjct: 256 GPLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIF 315

Query: 302 NRVFHNITDTHVVHHLFSTMPHYNAREATEAVKPILGEYYRLDRT 341
           N + H+I  THV+HHLF  +PHY+  +AT+A K +LG YYR  +T
Sbjct: 316 NNIHHDI-GTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKT 338

BLAST of MS010234.1 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-58
Identity = 124/326 (38.04%), Postives = 184/326 (56.44%), Query Frame = 0

Query: 16  PHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFPLLPHPLEYI 75
           P A PPF L+ I+ A+P HC+ ++  +S SY++ D   V++ F LA     L      +I
Sbjct: 90  PGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRD---VAIVFALAAGAAYL----NNWI 149

Query: 76  AWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYFSWKYSHRRH 135
            WP+YW+ QG     ++V+ H+CGH +FS+   ++ +VG + HS++LVPY  W+ SHR H
Sbjct: 150 VWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTH 209

Query: 136 HSNTGSLERDEVFVPRHKSKIPWYIKYFN--NPPGRLISL-LATLTLGWPLYLTFNVSGR 195
           H N G +E DE + P  +       K +N  + P R     L  + L +P YL     G+
Sbjct: 210 HQNHGHVENDESWHPMSE-------KIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGK 269

Query: 196 PYDRFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIYGVPLVI 255
                  HY P   +F  +E   +  S A   +MA +L  +    G + ++++YG+P  I
Sbjct: 270 K----GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWI 329

Query: 256 VNGFLVLITYLQH--THPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVVHH 315
              +L  +TYL H      LP Y   EW +LRG L T+DRDYG++N + H+I  THV+HH
Sbjct: 330 NVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVIHH 389

Query: 316 LFSTMPHYNAREATEAVKPILGEYYR 337
           LF  +PHY+  EATEA KP+LG+YYR
Sbjct: 390 LFPQIPHYHLVEATEAAKPVLGKYYR 396

BLAST of MS010234.1 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-56
Identity = 119/323 (36.84%), Postives = 175/323 (54.18%), Query Frame = 0

Query: 16  PHAKPPFTLSQIKKAVPPHCFDRSLLRSFSYLLSDLCFVSLFFYLAIACFPLLPHPLEYI 75
           P A PPF L+ I+ A+P HC+ ++   S SY++ D+  V  F   A+A +        ++
Sbjct: 83  PGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIV--FGLAAVAAY-----FNNWL 142

Query: 76  AWPIYWIFQGCTLTGVWVIAHECGHHAFSDYQLVDDIVGLIFHSALLVPYFSWKYSHRRH 135
            WP+YW  QG     ++V+ H+CGH +FS+   ++ + G + HS++LVPY  W+ SHR H
Sbjct: 143 LWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTH 202

Query: 136 HSNTGSLERDEVFVPRHKSKIPWYIKYFNNPPGRLISLLATLTLGWPLYLTFNVSGRPYD 195
           H N G +E DE + P  +S      K            L    L +P YL     G+   
Sbjct: 203 HQNHGHVENDESWHPLPES----IYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQ-- 262

Query: 196 RFACHYDPYGPIFTKREWLQIYISDAGIISMAFVLYRIAVAKGALWVMRIYGVPLVIVNG 255
               HY P   +F  +E   +  S A   +MA +L  +    G + ++++YG+P  I   
Sbjct: 263 --GSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVM 322

Query: 256 FLVLITYLQH--THPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVVHHLFS 315
           +L  +TYL H      LP Y   EW +LRG L T+DRDYG +N + H+I  THV+HHLF 
Sbjct: 323 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI-GTHVIHHLFP 382

Query: 316 TMPHYNAREATEAVKPILGEYYR 337
            +PHY+  EATEA KP+LG+YYR
Sbjct: 383 QIPHYHLVEATEAAKPVLGKYYR 389