MS010164.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS010164.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type domain-containing protein
Location	scaffold391: 350519 .. 353240 (-)
Sequence length	648
RNA-Seq Expression	MS010164.1
Synteny	MS010164.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGGAAAGAAGGTAGAGATTCAAGGAACGGGGCATGGGAGTCATGATGGGGCTTGTGCAATTTGCTTGAATAAGATAGCTCTTCAAGAAACTGCCTTAGTGAGAGGTTGCGAGCACGCCTATTGGTTTGTTCTAATTGATTAATCTTTCTTTTATCTATTGATTAATACTTTTTGGAGCTTGAATTTTGTCCTGCTACAATACATTAGGTAATTTTTTATATGTTCCTTTCCTGTTTTTTTATGGGAATCTTTTTTGTTGTAGTTATACAGATGGTTCAATGCTAATGCTGCTTTTATTACATATATTTCATTTCGTAGTTGTTTTATGCTTATATTGGAGATGGAGTTTATTAAGTTTTACGTTTTGATAAACTTTATAGGAGAAAAATTTCACTGTATCCGCATTGCCTCTGGTGCAATGTTTCTGATTGTACTTTGGGTTTCTGGGATTTAATGGAAAAGGTACGCTATGTTAGATTTATAACCAGAGCACCTTGGAGTTAGTGAAATGTAAGGTACAGAAGTCTTATGTGTAATCTATAATTTGATTTTCTTTGAAAAACGAAGTATCATAAACTTGCAAAACTCTTTGTTATTTAGTTTTGTTTAATGGATTAGTTATTGAAGTTAGAATGCTTCCTTGGTGAGACTTGGGTAGAAATTTGTTATCCTCGGTCAGTATTTTAAGTGATTGGGTAACGTGTCTTGGTTCTACATGCCAGTGGACACTCCAACCTTACATTTGTTAAGTACAATGGACACATGTCAGACACTTGTTAAATTGGGTACTTATCAAACACGGTTGAACATTTGTTAAGTATCATAGATGCATATTGAACACATATTGAACAATTATTGGTCATTGGAACTCAAGTGTTCAAGCATATGTTGAACAAGGCTTAGACGATTAAAAGATGATGTTTTACAATGTCGTGCCAAGCTAGTTAACCTTTCTGCACCTCCAAATCATGTCATTTCATTACATACTTTGATGCATTTCATGGCTGTTGAAAAGTTCCACTTTTTATGTTTTTATACTTACTTTTGGGAAGATTCCCCCTACTGCTGTTGCAGGGAACACTTTGTATATTTTCCACTTTCAGCTGGGAAATTTATTAGTAATAACTAAACCAAACAAAAATCGGAGAGGCATATGGTTAATGCAATTGTCAATTGTTTTGTAACCACTCAAGTATTAGAAAATTTTCCTTGTTATGCTTGTGAAATGATATTGCCTTTATAGTTGTACATATGGTAATTATCTTCTTTCATTTATCTATTATCTTGTTTTTGTGAAGTGTGACTTGCATCCTTCGATGGGCCTCGTACACTCAGAAACCGACTTGTCCACAATGTAAACATCCATTTGAGTTCTTGATTGTGCATCGTTCTCTTGATGGGGGGTATGTGTGTACATATTTTATAGTGATCTCCTAGCCATTAGCTTATTTGCTTTCTCTGAGATCCTTTAGTTTATTTGATAATTATCTTTTTAAAATGTGATCAATACTACAAATGTGAAATCATTCATCAATAACATAGTACGAGATATATATGTGATTCTCTTAAAATTTACATTTGGAAAGGTAAAAAGGTCCATGTGTACTTTATATAGAGGAAAACTATGGTGAAGGGCCGTGGCACTGAAAGTTTAAGTCATGCATGCTTATCTGTTCTTTTGTACAAATTTGGGGAAAAACAAGGATGCACTTTTCATAGGAAAATAATAATAATAATAAGTTCACTTTATCTCCTCCATCATGTCAAGTATCTATTGTCTATATTGCCTTTGTTTTATCTTCCATTTCTTTTTTATGAAAGCTTAGTTTCTTTTAAAAAATTCAATTATCTATATTTCAATAAGTTTTCTGTTAAGAATTACAAGCTCCATAGTTTTAAAAATAAATTCTCAGCTTAATTTCGATAATGGGAACTTGTATTCAAGAAAATATGAACCACTATTAAAAAGATTTGTAGTAGTTTTAGTTTTTAAATAATCCAAAGACAATTTTTATTATTAACTCAACTACCTATAAAGACACTATAGTGAGGTATAATAGAATAGGAAAGGTCTCGTGCAGATGTATGAAGTAATAAACTTTAGATCACTTTTGTTGGAAAGGTCCCAACTCTCATAAAAATTTCATTTGTGAGAGTAACACGGGAAAGTTTGGTTACAATTATGTGATGTGTTATGCAGCCCTTAACGTTTTGTTCTATAGAGAATGAGATTATGTGGTGCAAGTTATTTGTTATGCATTGTTCTTATTATTTGATCTGTATATTATCTGCATATTTTTAGCATCCATGACTACATGTTTGAGGAGAGTGTATGCCTGCTTTTGAGAGCTTCATGGTATATACCATTGGTTGTTGAAGACCGGGAAGAAACCTATGATGATCCTGAGGAGTACTATCCGTATGAAGATGATGACGAAGAGCTTGAGGAAGATTACCTGGGTGCATCCTCAAGTCTTCGCATCGGCAACCGTAGGTGGGGGGACAATGGTTATGTGAGGGCAGGTCGACAAGAAGCACGGCCAATTTTTAGGCCAAGTGTTCCAGAGTTTGCTGCGGCTAGCTCATCACGCGAGCCTACAAAGAAAGATGCTTCTAAAGATAAAACCGGTCGACGAGCTAAAAGAGCTTTAAAACGGGAAGCTGCCGATAAAGCAGCAGCAGCAAAGCATCAGGAACATCTAGCTAGGTTGGGTCGGAAT

mRNA sequence

ATAGGAAAGAAGGTAGAGATTCAAGGAACGGGGCATGGGAGTCATGATGGGGCTTGTGCAATTTGCTTGAATAAGATAGCTCTTCAAGAAACTGCCTTAGTGAGAGGTTGCGAGCACGCCTATTGTGTGACTTGCATCCTTCGATGGGCCTCGTACACTCAGAAACCGACTTGTCCACAATGTAAACATCCATTTGAGTTCTTGATTGTGCATCGTTCTCTTGATGGGGGCATCCATGACTACATGTTTGAGGAGAGTGTATGCCTGCTTTTGAGAGCTTCATGGTATATACCATTGGTTGTTGAAGACCGGGAAGAAACCTATGATGATCCTGAGGAGTACTATCCGTATGAAGATGATGACGAAGAGCTTGAGGAAGATTACCTGGGTGCATCCTCAAGTCTTCGCATCGGCAACCGTAGGTGGGGGGACAATGGTTATGTGAGGGCAGGTCGACAAGAAGCACGGCCAATTTTTAGGCCAAGTGTTCCAGAGTTTGCTGCGGCTAGCTCATCACGCGAGCCTACAAAGAAAGATGCTTCTAAAGATAAAACCGGTCGACGAGCTAAAAGAGCTTTAAAACGGGAAGCTGCCGATAAAGCAGCAGCAGCAAAGCATCAGGAACATCTAGCTAGGTTGGGTCGGAAT

Coding sequence (CDS)

ATAGGAAAGAAGGTAGAGATTCAAGGAACGGGGCATGGGAGTCATGATGGGGCTTGTGCAATTTGCTTGAATAAGATAGCTCTTCAAGAAACTGCCTTAGTGAGAGGTTGCGAGCACGCCTATTGTGTGACTTGCATCCTTCGATGGGCCTCGTACACTCAGAAACCGACTTGTCCACAATGTAAACATCCATTTGAGTTCTTGATTGTGCATCGTTCTCTTGATGGGGGCATCCATGACTACATGTTTGAGGAGAGTGTATGCCTGCTTTTGAGAGCTTCATGGTATATACCATTGGTTGTTGAAGACCGGGAAGAAACCTATGATGATCCTGAGGAGTACTATCCGTATGAAGATGATGACGAAGAGCTTGAGGAAGATTACCTGGGTGCATCCTCAAGTCTTCGCATCGGCAACCGTAGGTGGGGGGACAATGGTTATGTGAGGGCAGGTCGACAAGAAGCACGGCCAATTTTTAGGCCAAGTGTTCCAGAGTTTGCTGCGGCTAGCTCATCACGCGAGCCTACAAAGAAAGATGCTTCTAAAGATAAAACCGGTCGACGAGCTAAAAGAGCTTTAAAACGGGAAGCTGCCGATAAAGCAGCAGCAGCAAAGCATCAGGAACATCTAGCTAGGTTGGGTCGGAAT

Protein sequence

IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDDDEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARLGRN

Homology

[Close]

BLAST of MS010164.1 vs. NCBI nr
Match: XP_022153003.1 (uncharacterized protein LOC111020607 [Momordica charantia])

HSP 1 Score: 438.7 bits (1127), Expect = 2.9e-119
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ
Sbjct: 28  IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA
Sbjct: 148 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGRN 217
           SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGRN
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGRN 243

[Close]

BLAST of MS010164.1 vs. NCBI nr
Match: KAG6605938.1 (hypothetical protein SDJN03_03255, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035883.1 hypothetical protein SDJN02_02682 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 397.9 bits (1021), Expect = 5.6e-107
Identity = 192/215 (89.30%), Postives = 201/215 (93.49%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           +GKKVEIQGTGHGSHDG CAICLNKIALQETALVRGCEHAYC TCILRWASY + PTCPQ
Sbjct: 28  MGKKVEIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDG +HD+MFEESVCLLLRASWYIPL+VEDREETY+DP+EYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDREETYEDPDEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEE EE YLGASSSLRIGNRRWGDNGYVR GRQEARP+ R +VP  A ASSSREPTKKDA
Sbjct: 148 DEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRSTVPVHAGASSSREPTKKDA 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGR 216
           SKDKTGRRAKRALKREAADKAAAAKHQEHLAR GR
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQEHLARFGR 242

[Close]

BLAST of MS010164.1 vs. NCBI nr
Match: XP_022958365.1 (uncharacterized protein LOC111459609 [Cucurbita moschata])

HSP 1 Score: 394.8 bits (1013), Expect = 4.7e-106
Identity = 191/215 (88.84%), Postives = 200/215 (93.02%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           +GKKVEIQGTGHGSHDG CAICLNKIALQETALVRGCEHAYC TCILRWASY + PTCPQ
Sbjct: 28  MGKKVEIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDG +HD+MFEESVCLLLRASWYIPL+VEDREETY+DP+EYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDREETYEDPDEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEE EE YLGASSSLRIGNRRWGDNGYVR GRQEARP+ R +VP  A ASSSREPTKKDA
Sbjct: 148 DEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRSTVPVHAGASSSREPTKKDA 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGR 216
           SKDKTGRRAKRALKREAADKAAAAKHQE LAR GR
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQERLARFGR 242

[Close]

BLAST of MS010164.1 vs. NCBI nr
Match: XP_023533579.1 (uncharacterized protein LOC111795401 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 393.3 bits (1009), Expect = 1.4e-105
Identity = 190/215 (88.37%), Postives = 199/215 (92.56%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           +GKKVEIQGTGHGSHDG CAICLNKIALQETALVRGCEHAYC TCILRWASY + PTCPQ
Sbjct: 28  MGKKVEIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDG +HD+MFEESVCLLLRASWYIPL+VEDREETY+DP+EYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDREETYEDPDEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEE EE YLGASSSLRIGNRRWGDNGYVR GRQEARP+ R +VP  A ASSSREP KKD 
Sbjct: 148 DEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRSTVPVHAGASSSREPIKKDD 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGR 216
           SKDKTGRRAKRALKREAADKAAAAKHQEHLAR GR
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQEHLARFGR 242

[Close]

BLAST of MS010164.1 vs. NCBI nr
Match: XP_022995136.1 (uncharacterized protein LOC111490767 [Cucurbita maxima])

HSP 1 Score: 393.3 bits (1009), Expect = 1.4e-105
Identity = 189/215 (87.91%), Postives = 199/215 (92.56%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           +GKKVEI GTGHGSHDG CAICLNKIALQETALVRGCEHAYC TCILRWASY + PTCPQ
Sbjct: 28  MGKKVEIHGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDG +HD+MFEESVCLLLRASWY+PL+VEDREETY+DP+EYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYVPLIVEDREETYEDPDEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEE EE YLGASSSLRIGNRRWGDNGYVR GRQEARP+ R +VP  A ASSSREP KKDA
Sbjct: 148 DEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRSTVPVHAGASSSREPIKKDA 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGR 216
           SKDKTGRRAKRALKREAADKAAAAKHQEHLAR GR
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQEHLARFGR 242

[Close]

BLAST of MS010164.1 vs. ExPASy Swiss-Prot
Match: Q99590 (Protein SCAF11 OS=Homo sapiens OX=9606 GN=SCAF11 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 1.3e-05
Identity = 21/58 (36.21%), Postives = 32/58 (55.17%), Query Frame = 0


Query: 19 CAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDG 77
          C ICLN +  +E      C H +C+TCIL+WA      +CP  + PF+ +    +L+G
Sbjct: 41 CPICLNCLLEKEVGFPESCNHVFCMTCILKWAE--TLASCPIDRKPFQAVFKFSALEG 96

[Close]

BLAST of MS010164.1 vs. ExPASy Swiss-Prot
Match: Q60764 (Probable E3 ubiquitin-protein ligase makorin-3 OS=Mus musculus OX=10090 GN=Mkrn3 PE=2 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 9.2e-04
Identity = 23/78 (29.49%), Postives = 36/78 (46.15%), Query Frame = 0


Query: 3   KKVEIQGTGHGSHDGACAICLNKI------ALQETALVRGCEHAYCVTCILRWASYTQ-- 62
           + +E+      S D  C IC+  +      + +   ++  C H YC+ CI RW S TQ  
Sbjct: 331 RDMELSFAVQRSMDKVCGICMEVVYEKADPSDRRFGILFSCNHTYCLRCIRRWRSATQFE 390

Query: 63  ---KPTCPQCKHPFEFLI 70
                +CPQC+    F+I
Sbjct: 391 NRISKSCPQCRVSSGFVI 408

[Close]

BLAST of MS010164.1 vs. ExPASy TrEMBL
Match: A0A6J1DGD3 (uncharacterized protein LOC111020607 OS=Momordica charantia OX=3673 GN=LOC111020607 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.4e-119
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ
Sbjct: 28  IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA
Sbjct: 148 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGRN 217
           SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGRN
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGRN 243

[Close]

BLAST of MS010164.1 vs. ExPASy TrEMBL
Match: A0A6J1H396 (uncharacterized protein LOC111459609 OS=Cucurbita moschata OX=3662 GN=LOC111459609 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.3e-106
Identity = 191/215 (88.84%), Postives = 200/215 (93.02%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           +GKKVEIQGTGHGSHDG CAICLNKIALQETALVRGCEHAYC TCILRWASY + PTCPQ
Sbjct: 28  MGKKVEIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDG +HD+MFEESVCLLLRASWYIPL+VEDREETY+DP+EYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDREETYEDPDEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEE EE YLGASSSLRIGNRRWGDNGYVR GRQEARP+ R +VP  A ASSSREPTKKDA
Sbjct: 148 DEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRSTVPVHAGASSSREPTKKDA 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGR 216
           SKDKTGRRAKRALKREAADKAAAAKHQE LAR GR
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQERLARFGR 242

[Close]

BLAST of MS010164.1 vs. ExPASy TrEMBL
Match: A0A6J1K158 (uncharacterized protein LOC111490767 OS=Cucurbita maxima OX=3661 GN=LOC111490767 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.7e-106
Identity = 189/215 (87.91%), Postives = 199/215 (92.56%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQ 60
           +GKKVEI GTGHGSHDG CAICLNKIALQETALVRGCEHAYC TCILRWASY + PTCPQ
Sbjct: 28  MGKKVEIHGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQ 87

Query: 61  CKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDD 120
           CKHPFEFLIVHRSLDG +HD+MFEESVCLLLRASWY+PL+VEDREETY+DP+EYYPYEDD
Sbjct: 88  CKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYVPLIVEDREETYEDPDEYYPYEDD 147

Query: 121 DEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDA 180
           DEE EE YLGASSSLRIGNRRWGDNGYVR GRQEARP+ R +VP  A ASSSREP KKDA
Sbjct: 148 DEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRSTVPVHAGASSSREPIKKDA 207

Query: 181 SKDKTGRRAKRALKREAADKAAAAKHQEHLARLGR 216
           SKDKTGRRAKRALKREAADKAAAAKHQEHLAR GR
Sbjct: 208 SKDKTGRRAKRALKREAADKAAAAKHQEHLARFGR 242

[Close]

BLAST of MS010164.1 vs. ExPASy TrEMBL
Match: A0A6J1FXD4 (uncharacterized protein LOC111448431 OS=Cucurbita moschata OX=3662 GN=LOC111448431 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.5e-105
Identity = 193/214 (90.19%), Postives = 199/214 (92.99%), Query Frame = 0


Query: 3   KKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQCK 62
           KK+EIQGTGHGSHDGACAICLNKI+L ETALVRGCEHAYC TCILRWASYTQKPTCPQCK
Sbjct: 30  KKLEIQGTGHGSHDGACAICLNKISLPETALVRGCEHAYCTTCILRWASYTQKPTCPQCK 89

Query: 63  HPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDDDE 122
           HPFEFLIVHRSLDG IHDYMFEESVCLLLRASWY PL+V+DREETYDDPEEYYPYED DE
Sbjct: 90  HPFEFLIVHRSLDGSIHDYMFEESVCLLLRASWYEPLIVKDREETYDDPEEYYPYEDQDE 149

Query: 123 ELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDASK 182
           ELEE YLGAS SLRIGNRRWGDNGYVRAGRQEARP+ R   PEF+ ASSSREPT KDASK
Sbjct: 150 ELEEAYLGASPSLRIGNRRWGDNGYVRAGRQEARPVLR---PEFSGASSSREPTTKDASK 209

Query: 183 DKTGRRAKRALKREAADKAAAAKHQEHLARLGRN 217
           DKTGRRAKRALKREAADKAAAAKHQEHLAR G N
Sbjct: 210 DKTGRRAKRALKREAADKAAAAKHQEHLARFGLN 240

[Close]

BLAST of MS010164.1 vs. ExPASy TrEMBL
Match: A0A6J1JFP2 (uncharacterized protein LOC111484597 OS=Cucurbita maxima OX=3661 GN=LOC111484597 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.5e-101
Identity = 188/214 (87.85%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 3   KKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQCK 62
           KK+E+Q TGHGSH G+CAICLNKI+L ETALVRGCEHAYC TCILRWASYTQKPTCPQCK
Sbjct: 30  KKLEMQRTGHGSH-GSCAICLNKISLPETALVRGCEHAYCTTCILRWASYTQKPTCPQCK 89

Query: 63  HPFEFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPLVVEDREETYDDPEEYYPYEDDDE 122
           HPFEFLIVHRSLDG IHDYMFEESVCLLLRASWY PL+V+DREETYDDPEEYYPYED DE
Sbjct: 90  HPFEFLIVHRSLDGSIHDYMFEESVCLLLRASWYEPLIVKDREETYDDPEEYYPYEDQDE 149

Query: 123 ELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFAAASSSREPTKKDASK 182
           ELEE YLG S SLRIGNRRWGDNGYVRAGRQEARP+ R   PEF+ ASSSREPT KDASK
Sbjct: 150 ELEEAYLGDSPSLRIGNRRWGDNGYVRAGRQEARPVLR---PEFSGASSSREPTTKDASK 209

Query: 183 DKTGRRAKRALKREAADKAAAAKHQEHLARLGRN 217
           DKTGRRAKRALKREAADKAAAAKHQEHLAR G N
Sbjct: 210 DKTGRRAKRALKREAADKAAAAKHQEHLARFGLN 239

[Close]

BLAST of MS010164.1 vs. TAIR 10
Match: AT5G19430.1 (RING/U-box superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 3.0e-66
Identity = 137/223 (61.43%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 6   EIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQCKHPF 65
           EI     G+H G CAICL++I LQETA+V+GCEH YCVTCILRWAS  + PTCPQCKHPF
Sbjct: 33  EIHEPSFGNHGGCCAICLSEIPLQETAMVKGCEHTYCVTCILRWASCKESPTCPQCKHPF 92

Query: 66  EFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPL-VVEDREET---YDDPEEYYPYEDDD 125
           +FL VHR+LDG I D++FEESVCLLLRASW++PL VVE    +   +DD +  Y YED+D
Sbjct: 93  DFLSVHRALDGSIEDFLFEESVCLLLRASWFVPLDVVEQASYSHGYHDDFDIPYDYEDED 152

Query: 126 EELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFR----PSVPEFAAASSSREPTK 185
           ++L+E YL   SSLRIGNRRWG NG+VR+GRQEARP+ R     S    ++ SSS EP  
Sbjct: 153 DDLDEFYL-HGSSLRIGNRRWGGNGFVRSGRQEARPVQRYSGSGSSSSSSSGSSSSEPKD 212

Query: 186 KDA-SKDKTGRRAKRALKREAADKA----AAAKHQEHLARLGR 216
           K   + + TGRRAKRA+KREAA+KA    AAAKH+ HL RLGR
Sbjct: 213 KQVKTTNTTGRRAKRAMKREAANKAAEVTAAAKHEAHLVRLGR 254

[Close]

BLAST of MS010164.1 vs. TAIR 10
Match: AT5G19430.2 (RING/U-box superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 3.0e-66
Identity = 137/223 (61.43%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 6   EIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQCKHPF 65
           EI     G+H G CAICL++I LQETA+V+GCEH YCVTCILRWAS  + PTCPQCKHPF
Sbjct: 18  EIHEPSFGNHGGCCAICLSEIPLQETAMVKGCEHTYCVTCILRWASCKESPTCPQCKHPF 77

Query: 66  EFLIVHRSLDGGIHDYMFEESVCLLLRASWYIPL-VVEDREET---YDDPEEYYPYEDDD 125
           +FL VHR+LDG I D++FEESVCLLLRASW++PL VVE    +   +DD +  Y YED+D
Sbjct: 78  DFLSVHRALDGSIEDFLFEESVCLLLRASWFVPLDVVEQASYSHGYHDDFDIPYDYEDED 137

Query: 126 EELEEDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFR----PSVPEFAAASSSREPTK 185
           ++L+E YL   SSLRIGNRRWG NG+VR+GRQEARP+ R     S    ++ SSS EP  
Sbjct: 138 DDLDEFYL-HGSSLRIGNRRWGGNGFVRSGRQEARPVQRYSGSGSSSSSSSGSSSSEPKD 197

Query: 186 KDA-SKDKTGRRAKRALKREAADKA----AAAKHQEHLARLGR 216
           K   + + TGRRAKRA+KREAA+KA    AAAKH+ HL RLGR
Sbjct: 198 KQVKTTNTTGRRAKRAMKREAANKAAEVTAAAKHEAHLVRLGR 239

[Close]

BLAST of MS010164.1 vs. TAIR 10
Match: AT5G12310.1 (RING/U-box superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-63
Identity = 134/219 (61.19%), Postives = 162/219 (73.97%), Query Frame = 0


Query: 13  GSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPTCPQCKHPFEFLIVHR 72
           G+H G CAICL+ I LQETA+V+GCEHAYCVTCILRWASY +KPTCPQCK PF+FL VHR
Sbjct: 37  GNHGGCCAICLDTIPLQETAMVKGCEHAYCVTCILRWASYKEKPTCPQCKLPFDFLNVHR 96

Query: 73  SLDGGIHDYMFEESVCLLLRASWYIPLVVEDR-------EETYDDPEEYYPYEDDDEELE 132
           +LDG + D+MFEESVCLLLRASW+ PL   +R          +D P EY   ++DD++L+
Sbjct: 97  ALDGSVEDFMFEESVCLLLRASWFQPLEAVERVSDNDNFNYDFDIPPEYEEEDEDDDDLD 156

Query: 133 EDYLGASSSLRIGNRRWGDNGYVRAGRQEARPIFRPSVPEFA-----AASSSREPTKKDA 192
           E Y+   SSLR+GNRRWGDNG+VRAGRQEARP+   +    A     A+SSS EP +K +
Sbjct: 157 EFYM-QGSSLRLGNRRWGDNGFVRAGRQEARPVQHKNRGGQASGSEPASSSSCEPKEKTS 216

Query: 193 SKDKTGRRAKRALKREAADKA----AAAKHQEHLARLGR 216
           S   TGRRAKRA+KREAA+KA    AAAKH+  L RLGR
Sbjct: 217 SA-ITGRRAKRAMKREAANKAAEAVAAAKHEALLVRLGR 253

[Close]

BLAST of MS010164.1 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 42.0 bits (97), Expect = 7.2e-04
Identity = 22/73 (30.14%), Postives = 30/73 (41.10%), Query Frame = 0


Query: 1   IGKKVEIQGTGHGSHDGACAICLNKIALQETALVRGCEHAYCVTCILRWASYTQKPT--- 60
           I ++  +Q    G     CA+CL +     T    GCEH  CV C L   S +  P+   
Sbjct: 294 IAREFGLQSATIGDEVDICAVCLERTC---TVAAEGCEHQLCVRCALYLCSSSNVPSVTV 353

Query: 61  -------CPQCKH 64
                  CP C+H
Sbjct: 354 GPPGSIPCPLCRH 363

The following BLAST results are available for this feature:

BLAST of MS010164.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153003.1	2.9e-119	100.00	uncharacterized protein LOC111020607 [Momordica charantia]	[more]
KAG6605938.1	5.6e-107	89.30	hypothetical protein SDJN03_03255, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022958365.1	4.7e-106	88.84	uncharacterized protein LOC111459609 [Cucurbita moschata]	[more]
XP_023533579.1	1.4e-105	88.37	uncharacterized protein LOC111795401 [Cucurbita pepo subsp. pepo]	[more]
XP_022995136.1	1.4e-105	87.91	uncharacterized protein LOC111490767 [Cucurbita maxima]	[more]

BLAST of MS010164.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99590	1.3e-05	36.21	Protein SCAF11 OS=Homo sapiens OX=9606 GN=SCAF11 PE=1 SV=2	[more]
Q60764	9.2e-04	29.49	Probable E3 ubiquitin-protein ligase makorin-3 OS=Mus musculus OX=10090 GN=Mkrn3...	[more]

BLAST of MS010164.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGD3	1.4e-119	100.00	uncharacterized protein LOC111020607 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1H396	2.3e-106	88.84	uncharacterized protein LOC111459609 OS=Cucurbita moschata OX=3662 GN=LOC1114596...	[more]
A0A6J1K158	6.7e-106	87.91	uncharacterized protein LOC111490767 OS=Cucurbita maxima OX=3661 GN=LOC111490767...	[more]
A0A6J1FXD4	2.5e-105	90.19	uncharacterized protein LOC111448431 OS=Cucurbita moschata OX=3662 GN=LOC1114484...	[more]
A0A6J1JFP2	6.5e-101	87.85	uncharacterized protein LOC111484597 OS=Cucurbita maxima OX=3661 GN=LOC111484597...	[more]

BLAST of MS010164.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19430.1	3.0e-66	61.43	RING/U-box superfamily protein	[more]
AT5G19430.2	3.0e-66	61.43	RING/U-box superfamily protein	[more]
AT5G12310.1	1.1e-63	61.19	RING/U-box superfamily protein	[more]
AT5G07270.1	7.2e-04	30.14	XB3 ortholog 3 in Arabidopsis thaliana	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 19..61 e-value: 1.4E-5 score: 34.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 18..62 e-value: 1.2E-8 score: 35.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 19..62 score: 12.40076
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 2..67 e-value: 8.4E-12 score: 46.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 175..216
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 161..216
None	No IPR available	PANTHER	PTHR47361:SF4	RING/U-BOX SUPERFAMILY PROTEIN	coord: 9..215
None	No IPR available	PANTHER	PTHR47361	RING/U-BOX SUPERFAMILY PROTEIN	coord: 9..215
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 16..69
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 37..46

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS010164	MS010164	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS010164.1-cds	MS010164.1-cds-scaffold391:350519..350936	CDS
MS010164.1-cds	MS010164.1-cds-scaffold391:351836..351940	CDS
MS010164.1-cds	MS010164.1-cds-scaffold391:353116..353240	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS010164.1	MS010164.1-protein	polypeptide