MS009464.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS009464.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	thionin-like protein 2
Location	scaffold813: 1496644 .. 1496922 (+)
Sequence length	279
RNA-Seq Expression	MS009464.1
Synteny	MS009464.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTCAGAGCTTATTTTAGTTATGTTGGTTCTGAGCTTGGTTGCAGGCAAGTCCACAGCCTCATTCGGGAAGTGCTACGCCAAGTGCTTCGTTGTGTGCGCCATCACGCCGGGCATCCCCGTCGGCACGTGCGCTGCAAAGTGCCTCACAGATTGCCTCTTTCGTGCTGCATCGACCACAGCTGACTTCAACGATCAAATAGACACTCGTTACTTCTGTAAACTCGGCTGTGCCACTTCCCTCTGCACCAAATTCAGCACCAAGAAAGATCCAGGTTCG

mRNA sequence

AATTCAGAGCTTATTTTAGTTATGTTGGTTCTGAGCTTGGTTGCAGGCAAGTCCACAGCCTCATTCGGGAAGTGCTACGCCAAGTGCTTCGTTGTGTGCGCCATCACGCCGGGCATCCCCGTCGGCACGTGCGCTGCAAAGTGCCTCACAGATTGCCTCTTTCGTGCTGCATCGACCACAGCTGACTTCAACGATCAAATAGACACTCGTTACTTCTGTAAACTCGGCTGTGCCACTTCCCTCTGCACCAAATTCAGCACCAAGAAAGATCCAGGTTCG

Coding sequence (CDS)

AATTCAGAGCTTATTTTAGTTATGTTGGTTCTGAGCTTGGTTGCAGGCAAGTCCACAGCCTCATTCGGGAAGTGCTACGCCAAGTGCTTCGTTGTGTGCGCCATCACGCCGGGCATCCCCGTCGGCACGTGCGCTGCAAAGTGCCTCACAGATTGCCTCTTTCGTGCTGCATCGACCACAGCTGACTTCAACGATCAAATAGACACTCGTTACTTCTGTAAACTCGGCTGTGCCACTTCCCTCTGCACCAAATTCAGCACCAAGAAAGATCCAGGTTCG

Protein sequence

NSELILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADFNDQIDTRYFCKLGCATSLCTKFSTKKDPGS

Homology

[Close]

BLAST of MS009464.1 vs. NCBI nr
Match: XP_022151472.1 (thionin-like protein 2 [Momordica charantia])

HSP 1 Score: 186.8 bits (473), Expect = 8.4e-44
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1  NSELILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTT 60
          NSELILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTT
Sbjct: 3  NSELILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTT 62

Query: 61 ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP 92
          ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP
Sbjct: 63 ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP 93

[Close]

BLAST of MS009464.1 vs. NCBI nr
Match: XP_038899787.1 (thionin-like protein 2 [Benincasa hispida])

HSP 1 Score: 140.6 bits (353), Expect = 6.9e-30
Identity = 68/88 (77.27%), Postives = 76/88 (86.36%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
          +IL+  ++SLV G+STASFGKCYAKCFVVCAITPGIP+GTC AKCL DCLF  AS+  DF
Sbjct: 4  VILICFIMSLVVGRSTASFGKCYAKCFVVCAITPGIPIGTCGAKCLGDCLF-IASSPLDF 63

Query: 64 NDQIDTRYFCKLGCATSLCTKFSTKKDP 92
          N  +DT YFCKLGCATSLCTKFSTKKDP
Sbjct: 64 N-HLDTHYFCKLGCATSLCTKFSTKKDP 89

[Close]

BLAST of MS009464.1 vs. NCBI nr
Match: KAG7025781.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 136.3 bits (342), Expect = 1.3e-28
Identity = 63/88 (71.59%), Postives = 75/88 (85.23%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
          ++++  +LSLVAG+STASFGKCYAKCF+VCAITPG+P+GTC  KCL DCLF A++ T D 
Sbjct: 4  IVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPT-DL 63

Query: 64 NDQIDTRYFCKLGCATSLCTKFSTKKDP 92
          N  +DT YFCKLGCATSLCTKFSTK DP
Sbjct: 64 N-HLDTHYFCKLGCATSLCTKFSTKTDP 89

[Close]

BLAST of MS009464.1 vs. NCBI nr
Match: KAG6593441.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 135.2 bits (339), Expect = 2.9e-28
Identity = 63/88 (71.59%), Postives = 73/88 (82.95%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
          ++++  +LSLVAG+STASFGKCYAKCF+VCAITPG+P+GTC  KCL DCLF  AS   D 
Sbjct: 4  IVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLF-PASAPRDL 63

Query: 64 NDQIDTRYFCKLGCATSLCTKFSTKKDP 92
          N  +DT YFCKLGCATSLCTKFSTK DP
Sbjct: 64 N-HLDTHYFCKLGCATSLCTKFSTKTDP 89

[Close]

BLAST of MS009464.1 vs. NCBI nr
Match: XP_023000407.1 (thionin-like protein 2 [Cucurbita maxima])

HSP 1 Score: 134.8 bits (338), Expect = 3.8e-28
Identity = 63/88 (71.59%), Postives = 73/88 (82.95%), Query Frame = 0


Query: 4   LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
           ++++  +LSLVAG+STASFGKCYAKCF+VCAITPG+P+GTC  KCL DCLF  AS   D 
Sbjct: 39  VVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLF-LASAPRDL 98

Query: 64  NDQIDTRYFCKLGCATSLCTKFSTKKDP 92
           N  +DT YFCKLGCATSLCTKFSTK DP
Sbjct: 99  N-HLDTHYFCKLGCATSLCTKFSTKTDP 124

[Close]

BLAST of MS009464.1 vs. ExPASy Swiss-Prot
Match: A8MRP4 (Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-04
Identity = 28/91 (30.77%), Postives = 47/91 (51.65%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTAS--FGKCYAKCFVVCAITPGIP-VGTCAAKCLTDCLFRAASTT 63
          +++VM++ +L+A  +     F +CY  C V C      P    C   C  +CL + +  +
Sbjct: 5  VMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPS 64

Query: 64 ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP 92
             N+  ++ YFCKLGCAT  C   S+ ++P
Sbjct: 65 VSSNNIDESDYFCKLGCATYHCVSLSSIQNP 95

[Close]

BLAST of MS009464.1 vs. ExPASy TrEMBL
Match: A0A6J1DDL8 (thionin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019402 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.1e-44
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1  NSELILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTT 60
          NSELILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTT
Sbjct: 3  NSELILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTT 62

Query: 61 ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP 92
          ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP
Sbjct: 63 ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP 93

[Close]

BLAST of MS009464.1 vs. ExPASy TrEMBL
Match: A0A0A0KC18 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446640 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.4e-30
Identity = 68/89 (76.40%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
          ++L+  +LSLVAG+STASFGKCYAKCF+VCAITPGIPVGTC AKCL DCLF  AS+  D 
Sbjct: 4  VVLICFILSLVAGRSTASFGKCYAKCFIVCAITPGIPVGTCGAKCLADCLF-IASSPMDL 63

Query: 64 NDQIDTRYFCKLGCATSLCTKFSTKKDPG 93
          N  +DT YFCKLGCATS CTKFSTKKDPG
Sbjct: 64 N-YMDTHYFCKLGCATSRCTKFSTKKDPG 90

[Close]

BLAST of MS009464.1 vs. ExPASy TrEMBL
Match: A0A6J1KDK0 (thionin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111494667 PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.8e-28
Identity = 63/88 (71.59%), Postives = 73/88 (82.95%), Query Frame = 0


Query: 4   LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
           ++++  +LSLVAG+STASFGKCYAKCF+VCAITPG+P+GTC  KCL DCLF  AS   D 
Sbjct: 39  VVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLF-LASAPRDL 98

Query: 64  NDQIDTRYFCKLGCATSLCTKFSTKKDP 92
           N  +DT YFCKLGCATSLCTKFSTK DP
Sbjct: 99  N-HLDTHYFCKLGCATSLCTKFSTKTDP 124

[Close]

BLAST of MS009464.1 vs. ExPASy TrEMBL
Match: A0A6J1BFU4 (thionin-like protein 2 OS=Herrania umbratica OX=108875 GN=LOC110426315 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.3e-17
Identity = 47/89 (52.81%), Postives = 63/89 (70.79%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
          +++V LVL L+ G+STASF  CY  CF++C ITP   V +C+ KCL DC+  ++S    F
Sbjct: 9  VLMVYLVLGLLVGQSTASFQTCYMGCFILCVITPNNTVFSCSVKCLKDCIIPSSS----F 68

Query: 64 NDQIDTRYFCKLGCATSLCTKFSTKKDPG 93
              DT+YFCKLGCATSLCT  S+K++PG
Sbjct: 69 PSGKDTQYFCKLGCATSLCTDLSSKENPG 93

[Close]

BLAST of MS009464.1 vs. ExPASy TrEMBL
Match: A0A067E0U5 (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033731mg PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.3e-17
Identity = 46/88 (52.27%), Postives = 60/88 (68.18%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 63
          L++V L+  L+ G+S A+F +CY  CF++C ITPG  +G+CAAKCL DC+F     T   
Sbjct: 9  LLMVYLMTGLLVGQSQAAFKECYETCFIICYITPGTTLGSCAAKCLKDCIFPPKLHTLSL 68

Query: 64 NDQIDTRYFCKLGCATSLCTKFSTKKDP 92
              DT YFCKLGCATSLCT  S+K +P
Sbjct: 69 Q---DTEYFCKLGCATSLCTNLSSKDNP 93

[Close]

BLAST of MS009464.1 vs. TAIR 10
Match: AT1G12663.1 (Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant thionin (PR-13) family with the following members: At1g66100, At5g36910, At1g72260, At2g15010, At1g12663, At1g12660. )

HSP 1 Score: 47.0 bits (110), Expect = 9.7e-06
Identity = 28/91 (30.77%), Postives = 47/91 (51.65%), Query Frame = 0


Query: 4  LILVMLVLSLVAGKSTAS--FGKCYAKCFVVCAITPGIP-VGTCAAKCLTDCLFRAASTT 63
          +++VM++ +L+A  +     F +CY  C V C      P    C   C  +CL + +  +
Sbjct: 5  VMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPS 64

Query: 64 ADFNDQIDTRYFCKLGCATSLCTKFSTKKDP 92
             N+  ++ YFCKLGCAT  C   S+ ++P
Sbjct: 65 VSSNNIDESDYFCKLGCATYHCVSLSSIQNP 95

The following BLAST results are available for this feature:

BLAST of MS009464.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151472.1	8.4e-44	100.00	thionin-like protein 2 [Momordica charantia]	[more]
XP_038899787.1	6.9e-30	77.27	thionin-like protein 2 [Benincasa hispida]	[more]
KAG7025781.1	1.3e-28	71.59	Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6593441.1	2.9e-28	71.59	Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023000407.1	3.8e-28	71.59	thionin-like protein 2 [Cucurbita maxima]	[more]

BLAST of MS009464.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8MRP4	1.4e-04	30.77	Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1	[more]

BLAST of MS009464.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DDL8	4.1e-44	100.00	thionin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019402 PE=4 SV=1	[more]
A0A0A0KC18	4.4e-30	76.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446640 PE=4 SV=1	[more]
A0A6J1KDK0	1.8e-28	71.59	thionin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111494667 PE=4 SV=1	[more]
A0A6J1BFU4	3.3e-17	52.81	thionin-like protein 2 OS=Herrania umbratica OX=108875 GN=LOC110426315 PE=4 SV=1	[more]
A0A067E0U5	3.3e-17	52.27	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033731mg PE=4 SV=1	[more]

BLAST of MS009464.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12663.1	9.7e-06	30.77	Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant t...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038975	Thionin-like protein	PANTHER	PTHR36312	THIONIN-LIKE PROTEIN 1	coord: 3..92
None	No IPR available	PANTHER	PTHR36312:SF1	THIONIN-LIKE PROTEIN 1	coord: 3..92

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS009464	MS009464	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS009464.1-cds	MS009464.1-cds-scaffold813:1496644..1496922	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS009464.1	MS009464.1-protein	polypeptide