MS008858.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS008858.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionOrnithine carbamoyltransferase
Locationscaffold4: 4312532 .. 4315790 (-)
Sequence length1137
RNA-Seq ExpressionMS008858.1
SyntenyMS008858.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCGATTTGTTCTTACTCCACTGTACGATCGGAGAAAAACTCCCTGACTACTCAGTTCTACTCCGGCGACAGTCTTCGGCGATCGGTGAAGTTTTTGGGCGAGCTTTCAGGCTTTTCTGTGGCTTCTCCGGCTAGGTTTCCTGCTCGTTTTCCTGCAATCTCTTGCCAGACATCTGCAACTTCCCCTTCTTCCGTCGTATCAAAAGGTGCATGTCTGTCTTCGATTTAACTTCGTAGTTTATGATATTATTGCTATAATTTTCATTTCGACTAAGGTTTTTGCGTTTCTTGCTTAATAGACTCTGAAGTTAGGGTTTATCGATTGTGAGTGATGACGAAATTTATATTGCTCTATCACTATTCTTATGTAGTGCGTGAAGTTTGATAAGCTGTTATTATCAGACGGTGTATACGCCTTTTGGTATCGGTTTGAATGGTGCTCTGTTTAAGTGATATACATAATCCATCTTCTTCTGAACCGACCGATCTCTGACGCTTGCATTGGCGATATGCAAGATCGGCTGTTTCAGTGTCTATGGCTTGAGAACAGATTATGGTGTTTAGTCTTCCGCTCTTTCTCATGGGAAACAAATCATTGTAAAGGCATGCAATTCAATGAATACACGGATGTATGCTTTTGGTTGTCTGACGTTTGTTCTCTGTTGTATTATTTTTTCACAGTGAAAGCAGGGAAGAAGGATTTTCTTCACCTTAATGATTTTGATAAATCCACCCTCTTGAAGATCTTAGACCGCGCAGTTGAAGTTAAGGCACAGCTGAAATCTGGAGATAGGTCATTTGTCCCATTTAAAGGGAAGTCAATGGCTATGATCTTTGCAAAGCCATCCATGAGAACAAGAGTTTCATTTGAGACTGGTTTCTTCTTGCTAGGGGGCCATGCCATATATTTAGGGCCAAACGATATCCAAATGGGGAAAAGAGAGGAAACTAGGGATGTTGCTCGTGTTTTGTCTCGCTATAATGATGTCATCATGGCTCGTGTCTTTGCTCATCAGGTATGGTGGCCAGTTTTTTACCTTCTTAGTTCCAAGTGACAACATGTAGAGATATGAACCTGGAACGAGGTGTACTCAAGCTAGTTTTGCTTTATCAATTGGGTTCCGACCTTATTAGTCAAAGGACTTGAAGTTTCCTTAACATTTCTCTTCCAATAGATAGCTAAGCTATGTAGAATTATACACCTAATGAGGTTTATGCATCCATTGGTATGAAGTCACCTATTTTACAAGGTCGGAACCTTGCCCTGGTAGCCTTTGTATACTTGCACAAAACTCAGACATTTAGACATAAGATTGCTGTGGTGCATATACATTCACGTAGGCATAGTACTAATCATATTTGGTTTGTCTCAAAATAATTTTACTGTTACAATAATAGCTTATTTGATAACAAAGTTATCAATTCTTCAATTATTTAGTTGGTGCCCTATTCCAATTTGAGGTGGGTTAGCTGATTGTGGCAAATACTAGATACAGCAAAAAGCACATGCGGCCTTTCCAACATGGGAAAGATGTGTGAGGGGGAAATAATCTATTATTTAAACCTCTTTTCTCATTGGTGGGTTTCTAGTTGGGAGAAAACAAGAGCAAATTAAAAGCCAAATGGCCCTGCCTATCCCCTAGGAGATCAGGGAAAAGTGTCGGATTTCACATTACCTAATAGAGTGTTTCCAGTCCTGCCATATATATGTGGGCCATGATTGCTTCTATTTCATTTTTCTTCCGTTGCTGCCATTAGTAGTTCAGATGTCTCTCTACTGGGCTTTATACTATCATTTTGTTGCAGGACATTCTTGATTTAGCCAAATATGCCACTGTGCCGGTAATAAATGGCCTGACGGATTACAACCATCCTTGCCAACTAATGGCTGATGCACTCACTATTATTGAACACATTGGCAAGTTAGAAGGAGTCAAGGTTTGGCTGTGCCTCTTTACTGTGAACCGTTATATTAGATATAGAATCTATTTCCATAATGCTTTTCAAAGAATTGAGCGGGTGATAATGAAAATTTAGGTTGTCTATGTTGGAGACGGAAATAACATGGTGCACTCGTGGTTGTTGTTGGCATCAGTTGTGCCTATGCACTTTGTTTGTGCCTGTCCTAAAGGTTTTGAGCCGGATAAGAATACAGTTGAAAAGGCTCAGCAGGCTGGAATTAGCAAGATTGAGATAACCAATGATCCCAAGGAAGCAGTGAAAGGCGCTGATGTTGTGTACTCAGATGTCTGGGCCAGCATGGGACAAAAGGAGGAGGCTGAATATCGGCGTCAAGCGTTTCAAGGCTTTCAGGTAGTTGTTGGAGCATTTCTTTTTAATAAACACACTTCAAGCTAATTGGTCCGACTTCATATTGAGTTGTTTGATTTTGATCTTTTATGCCATCATTTGGGACGGAAAATTAAAAAATGGCCTCTTTGCTTCCCTTTTTCTGTGCTGAATACCACAGCTAACTACTAGCTCGCATGTTCACCTCTCCTGTTGTATTTTTGTTAGCTGTAACTTAGGTTGAAAGTAATATTCGTACCGATGAAATAATGTAGTTAGTGCAACATGGTCACAAATTAACTACACAAAGACAACTGTGTCTGGAAAATGCACTATCCCTTTTTCTAACCGAATGAAACGGGATTTCATTGAGGAAAATGCAGAAGTGATGGCTGTAGAAAAGGCTAACTTGAGTAGCTTAAATTTCAGGCATGCCTAACTCACGGGCTAAATATATGCCCGAATCCAAACCCTCTCTCAATACAACCCCTGGAAAGTTGCTCCAACTTTTCCACCCTTTACATTCATGGATTAATAGGATTCGATGTTGGAGATGCTGCAATTGTACAGTAATGGTATAATATTGAAATCGTTGTGTTCTCTTTGACACTCAGTAGAACAGACTAGCTACTCAGAATTATCACCCGACACAAACATCAGAGAGCACCGAGCAGAACTTGATCTTCCATTTGGATTTTTGAAACATTGATCTTTTGGATGCATACTGTATTACTTGACGGTTAAATTCTGCAGCTGTAATGACATTCTCTGCGTTATCGTTGCAGGTTAACGAAGAGCTCATGAATTTAGCAGGTTCGAAAGCTTATTTCATGCATTGCCTACCAGCAGAGAGAGGCGTAGAGGTAACGGATGGAGTAATTGAAGCTCCCAACTCCATTGTCTTTCCACAAGCTGAGAATCGAATGCACGCACAGAATGCTATAATGCTTCATGTTCTTGGCTTA

mRNA sequence

ATGGCAGCGATTTGTTCTTACTCCACTGTACGATCGGAGAAAAACTCCCTGACTACTCAGTTCTACTCCGGCGACAGTCTTCGGCGATCGGTGAAGTTTTTGGGCGAGCTTTCAGGCTTTTCTGTGGCTTCTCCGGCTAGGTTTCCTGCTCGTTTTCCTGCAATCTCTTGCCAGACATCTGCAACTTCCCCTTCTTCCGTCGTATCAAAAGTGAAAGCAGGGAAGAAGGATTTTCTTCACCTTAATGATTTTGATAAATCCACCCTCTTGAAGATCTTAGACCGCGCAGTTGAAGTTAAGGCACAGCTGAAATCTGGAGATAGGTCATTTGTCCCATTTAAAGGGAAGTCAATGGCTATGATCTTTGCAAAGCCATCCATGAGAACAAGAGTTTCATTTGAGACTGGTTTCTTCTTGCTAGGGGGCCATGCCATATATTTAGGGCCAAACGATATCCAAATGGGGAAAAGAGAGGAAACTAGGGATGTTGCTCGTGTTTTGTCTCGCTATAATGATGTCATCATGGCTCGTGTCTTTGCTCATCAGGACATTCTTGATTTAGCCAAATATGCCACTGTGCCGGTAATAAATGGCCTGACGGATTACAACCATCCTTGCCAACTAATGGCTGATGCACTCACTATTATTGAACACATTGGCAAGTTAGAAGGAGTCAAGGTTGTCTATGTTGGAGACGGAAATAACATGGTGCACTCGTGGTTGTTGTTGGCATCAGTTGTGCCTATGCACTTTGTTTGTGCCTGTCCTAAAGGTTTTGAGCCGGATAAGAATACAGTTGAAAAGGCTCAGCAGGCTGGAATTAGCAAGATTGAGATAACCAATGATCCCAAGGAAGCAGTGAAAGGCGCTGATGTTGTGTACTCAGATGTCTGGGCCAGCATGGGACAAAAGGAGGAGGCTGAATATCGGCGTCAAGCGTTTCAAGGCTTTCAGGTAGTTAACGAAGAGCTCATGAATTTAGCAGGTTCGAAAGCTTATTTCATGCATTGCCTACCAGCAGAGAGAGGCGTAGAGGTAACGGATGGAGTAATTGAAGCTCCCAACTCCATTGTCTTTCCACAAGCTGAGAATCGAATGCACGCACAGAATGCTATAATGCTTCATGTTCTTGGCTTA

Coding sequence (CDS)

ATGGCAGCGATTTGTTCTTACTCCACTGTACGATCGGAGAAAAACTCCCTGACTACTCAGTTCTACTCCGGCGACAGTCTTCGGCGATCGGTGAAGTTTTTGGGCGAGCTTTCAGGCTTTTCTGTGGCTTCTCCGGCTAGGTTTCCTGCTCGTTTTCCTGCAATCTCTTGCCAGACATCTGCAACTTCCCCTTCTTCCGTCGTATCAAAAGTGAAAGCAGGGAAGAAGGATTTTCTTCACCTTAATGATTTTGATAAATCCACCCTCTTGAAGATCTTAGACCGCGCAGTTGAAGTTAAGGCACAGCTGAAATCTGGAGATAGGTCATTTGTCCCATTTAAAGGGAAGTCAATGGCTATGATCTTTGCAAAGCCATCCATGAGAACAAGAGTTTCATTTGAGACTGGTTTCTTCTTGCTAGGGGGCCATGCCATATATTTAGGGCCAAACGATATCCAAATGGGGAAAAGAGAGGAAACTAGGGATGTTGCTCGTGTTTTGTCTCGCTATAATGATGTCATCATGGCTCGTGTCTTTGCTCATCAGGACATTCTTGATTTAGCCAAATATGCCACTGTGCCGGTAATAAATGGCCTGACGGATTACAACCATCCTTGCCAACTAATGGCTGATGCACTCACTATTATTGAACACATTGGCAAGTTAGAAGGAGTCAAGGTTGTCTATGTTGGAGACGGAAATAACATGGTGCACTCGTGGTTGTTGTTGGCATCAGTTGTGCCTATGCACTTTGTTTGTGCCTGTCCTAAAGGTTTTGAGCCGGATAAGAATACAGTTGAAAAGGCTCAGCAGGCTGGAATTAGCAAGATTGAGATAACCAATGATCCCAAGGAAGCAGTGAAAGGCGCTGATGTTGTGTACTCAGATGTCTGGGCCAGCATGGGACAAAAGGAGGAGGCTGAATATCGGCGTCAAGCGTTTCAAGGCTTTCAGGTAGTTAACGAAGAGCTCATGAATTTAGCAGGTTCGAAAGCTTATTTCATGCATTGCCTACCAGCAGAGAGAGGCGTAGAGGTAACGGATGGAGTAATTGAAGCTCCCAACTCCATTGTCTTTCCACAAGCTGAGAATCGAATGCACGCACAGAATGCTATAATGCTTCATGTTCTTGGCTTA

Protein sequence

MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTSATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAMIFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFAHQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSWLLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWASMGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFPQAENRMHAQNAIMLHVLGL
Homology
BLAST of MS008858.1 vs. NCBI nr
Match: XP_022137066.1 (ornithine carbamoyltransferase, chloroplastic [Momordica charantia])

HSP 1 Score: 748.0 bits (1930), Expect = 3.9e-212
Identity = 378/379 (99.74%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS
Sbjct: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM
Sbjct: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW
Sbjct: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRRQAFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRQAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. NCBI nr
Match: XP_038899565.1 (ornithine carbamoyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 692.6 bits (1786), Expect = 1.9e-195
Identity = 344/379 (90.77%), Postives = 364/379 (96.04%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+STVRS+KNS +T FYS  SLRRSVKF GE+SGFSVASP  FPARFP+ISCQTS
Sbjct: 1   MAAICSHSTVRSDKNSFSTHFYSSRSLRRSVKFFGEVSGFSVASPPPFPARFPSISCQTS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           ATSP SVV+KVKA KKDFLHL+DFDKSTLLKILDRA+EVKAQLKSGDR+F+PFKGK+MAM
Sbjct: 61  ATSPPSVVAKVKAEKKDFLHLSDFDKSTLLKILDRAIEVKAQLKSGDRTFLPFKGKTMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARVF 
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARVFG 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKY+TVPVINGLTDYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNMVHSW
Sbjct: 181 HQDILDLAKYSTVPVINGLTDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMVHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASVVP+HFVCACPKGFEPDK TVEKA+QAG+SKIEIT+DPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVVPLHFVCACPKGFEPDKITVEKARQAGVSKIEITHDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRRQ FQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRQVFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. NCBI nr
Match: XP_022941317.1 (ornithine carbamoyltransferase, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 689.1 bits (1777), Expect = 2.1e-194
Identity = 343/379 (90.50%), Postives = 364/379 (96.04%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+S VRSEK+SL T FYS   +RRSVKF G LSGFSVASPARFPARFP+I+CQTS
Sbjct: 1   MAAICSHSIVRSEKSSLPTHFYSSRGIRRSVKFSGGLSGFSVASPARFPARFPSITCQTS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           AT PS VVSKVKA KKDFLHL+DFDKSTL+KILDRA+EVKAQLKSGDR+F+PF+GK+MAM
Sbjct: 61  ATFPSPVVSKVKAEKKDFLHLSDFDKSTLMKILDRAIEVKAQLKSGDRTFLPFEGKTMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARVF 
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARVFG 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGLTDYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNMVHSW
Sbjct: 181 HQDILDLAKYATVPVINGLTDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMVHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASVVP+HFVCACPKGFEPDKNTVEKA+QAGISKIEI++DPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVVPLHFVCACPKGFEPDKNTVEKARQAGISKIEISHDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRR+AFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRKAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. NCBI nr
Match: XP_023536248.1 (ornithine carbamoyltransferase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 684.9 bits (1766), Expect = 4.0e-193
Identity = 341/379 (89.97%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+ST+RS+KNSL T FYS  SLRRSVKF GELSGFSVA P RFPA FP+ISCQ+S
Sbjct: 1   MAAICSHSTLRSDKNSLPTHFYSARSLRRSVKFFGELSGFSVAYPRRFPAGFPSISCQSS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           AT P SVVS+VKA KKDFLHL+DFDKSTLLKILDRAVEVKA+LKSGDR+F+PFKGK+MAM
Sbjct: 61  ATYPPSVVSQVKAEKKDFLHLSDFDKSTLLKILDRAVEVKARLKSGDRTFLPFKGKTMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARVF 
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARVFG 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGL+DYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNMVHSW
Sbjct: 181 HQDILDLAKYATVPVINGLSDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMVHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASV+P HFVCACPKGFEPDK+TV+KA+QAG+SKIEITNDPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVIPFHFVCACPKGFEPDKHTVDKARQAGVSKIEITNDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRRQAFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTD VIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRQAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDEVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. NCBI nr
Match: XP_022981986.1 (ornithine carbamoyltransferase, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 684.9 bits (1766), Expect = 4.0e-193
Identity = 341/379 (89.97%), Postives = 363/379 (95.78%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+S V+SEK++L T FYS  S RRSVKF G LSGFSVAS ARFPARFP+I+CQTS
Sbjct: 1   MAAICSHSIVQSEKSALPTHFYSSRSFRRSVKFSGGLSGFSVASSARFPARFPSITCQTS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           ATSPS VVSKVKA KKDFLHL+DFDKSTL+KILDRA+EVKAQLKSGDR+F+PF+GK+MAM
Sbjct: 61  ATSPSPVVSKVKAEKKDFLHLSDFDKSTLMKILDRAIEVKAQLKSGDRTFLPFEGKTMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARVF 
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARVFG 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGLTDYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNM HSW
Sbjct: 181 HQDILDLAKYATVPVINGLTDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMAHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASVVP+HFVCACPKGFEPDKNTVEKA+QAGISKIEI++DPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVVPLHFVCACPKGFEPDKNTVEKARQAGISKIEISHDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRR+AFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRKAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. ExPASy Swiss-Prot
Match: Q43814 (Ornithine carbamoyltransferase, chloroplastic OS=Pisum sativum OX=3888 GN=ARGF PE=2 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 2.9e-154
Identity = 281/374 (75.13%), Postives = 321/374 (85.83%), Query Frame = 0

Query: 7   YSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTSA--TSP 66
           ++TV S K  L+    S  + R S      LS  S +SP+  P R   ISCQ S+   + 
Sbjct: 11  FTTVGSHKPYLSP---SSHNFRHSPSV--SLSSSSSSSPS--PLR--RISCQASSAPAAE 70

Query: 67  SSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAMIFAK 126
           S++ +KV  G KDF+H++DFDK T+LKILDRA+EVK  LKSGDR+F PF+GK+M+MIFAK
Sbjct: 71  STLTAKVGNGLKDFIHIDDFDKETILKILDRAIEVKTLLKSGDRTFRPFEGKTMSMIFAK 130

Query: 127 PSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFAHQDI 186
           PSMRTRVSFETGF LLGGHAIYLGPNDIQMGKREETRDVARVLSRYND+IMARVF+HQDI
Sbjct: 131 PSMRTRVSFETGFSLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDIIMARVFSHQDI 190

Query: 187 LDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSWLLLA 246
           LDLAKYA+VPVINGLTDYNHP Q+MADALT+IEHIG+ EG KVVYVGDGNN+VHSWLLLA
Sbjct: 191 LDLAKYASVPVINGLTDYNHPVQIMADALTMIEHIGRFEGTKVVYVGDGNNIVHSWLLLA 250

Query: 247 SVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWASMGQK 306
           +VVP HFVCACPKGFEPD  TVEKA++AGISKIEI++DPKEAV+GADVVYSDVWASMGQK
Sbjct: 251 AVVPFHFVCACPKGFEPDAKTVEKARKAGISKIEISHDPKEAVRGADVVYSDVWASMGQK 310

Query: 307 EEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFPQAEN 366
           EEA YRR+AF+GFQ V++ LM+ AGSKA+FMHCLPAERGVEVTD V+EAP SIVFPQAEN
Sbjct: 311 EEAAYRREAFKGFQ-VDQNLMDAAGSKAFFMHCLPAERGVEVTDEVVEAPYSIVFPQAEN 370

Query: 367 RMHAQNAIMLHVLG 379
           RMHAQNAIMLHVLG
Sbjct: 371 RMHAQNAIMLHVLG 374

BLAST of MS008858.1 vs. ExPASy Swiss-Prot
Match: O50039 (Ornithine transcarbamylase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OTC PE=1 SV=2)

HSP 1 Score: 543.1 bits (1398), Expect = 2.5e-153
Identity = 283/383 (73.89%), Postives = 324/383 (84.60%), Query Frame = 0

Query: 2   AAICSY-STVRSEK---NSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISC 61
           AA+ S+ ST RS     +S ++ F+ G +LRR        S  S+ SPA    R   +SC
Sbjct: 3   AAMASHVSTARSPALSFSSSSSSFFPGTTLRR-------FSAVSLPSPALPRLR---VSC 62

Query: 62  Q-TSATSPSSVVS-KVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKG 121
           Q +S TSPSS    K K+  KDFL ++DFD +T+  ILD+A EVKA LKSG+R+++PFKG
Sbjct: 63  QASSVTSPSSPSDVKGKSDLKDFLAIDDFDTATIKTILDKASEVKALLKSGERNYLPFKG 122

Query: 122 KSMAMIFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIM 181
           KSM+MIFAKPSMRTRVSFETGFFLLGGHA+YLGPNDIQMGKREETRDVARVLSRYND+IM
Sbjct: 123 KSMSMIFAKPSMRTRVSFETGFFLLGGHALYLGPNDIQMGKREETRDVARVLSRYNDIIM 182

Query: 182 ARVFAHQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNN 241
           ARVFAHQDILDLA Y++VPV+NGLTD+NHPCQ+MADALT+IEHIG++EG KVVYVGDGNN
Sbjct: 183 ARVFAHQDILDLANYSSVPVVNGLTDHNHPCQIMADALTMIEHIGQVEGTKVVYVGDGNN 242

Query: 242 MVHSWLLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYS 301
           MVHSWL LASV+P HFVCACPKG+EPDK  V KA+QAG+SKIEITNDPKEAV GADVVYS
Sbjct: 243 MVHSWLELASVIPFHFVCACPKGYEPDKERVSKAKQAGLSKIEITNDPKEAVIGADVVYS 302

Query: 302 DVWASMGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPN 361
           DVWASMGQK+EAE RR+AFQGFQ V+E LM LAG KAYFMHCLPAERGVEVT+GV+EAP 
Sbjct: 303 DVWASMGQKDEAEARRKAFQGFQ-VDEALMKLAGQKAYFMHCLPAERGVEVTNGVVEAPY 362

Query: 362 SIVFPQAENRMHAQNAIMLHVLG 379
           SIVFPQAENRMHAQNAIMLH+LG
Sbjct: 363 SIVFPQAENRMHAQNAIMLHLLG 374

BLAST of MS008858.1 vs. ExPASy Swiss-Prot
Match: Q5JI16 (Ornithine carbamoyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=argF PE=3 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.9e-85
Identity = 163/311 (52.41%), Postives = 215/311 (69.13%), Query Frame = 0

Query: 71  VKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAMIFAKPSMRTR 130
           V    +D L L DF +  L  IL  A  +K   K G    V  +GK++AMIF KPS RTR
Sbjct: 3   VSLAGRDVLCLQDFTREELETILKTAEMMKIWNKIGKPHRV-LEGKTLAMIFQKPSTRTR 62

Query: 131 VSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFAHQDILDLAKY 190
           +SFE G + LGG+ +YL   D+Q+ + E   D ARVLSRY D IMARVF H+D+ DLAKY
Sbjct: 63  ISFEVGIYQLGGYGLYLNAQDLQLRRGETIADTARVLSRYVDGIMARVFDHKDVEDLAKY 122

Query: 191 ATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSWLLLASVVPMH 250
           A+VPVINGL+D++HPCQ +AD  TI+E  G+++G+K+VYVGDGNN+ HS ++  + +  +
Sbjct: 123 ASVPVINGLSDFSHPCQALADYQTILEKKGRIQGLKIVYVGDGNNVAHSLMIAGTKLGAN 182

Query: 251 FVCACPKGFEPDKNTVEKAQQAGI---SKIEITNDPKEAVKGADVVYSDVWASMGQKEEA 310
            V A P+G+EPD   ++ A+Q         E+ +DP +AVK ADV+Y+DVWASMGQ+ EA
Sbjct: 183 VVVATPEGYEPDPKVIKWAEQNAAESGGSFELLHDPVQAVKDADVIYTDVWASMGQEAEA 242

Query: 311 EYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFPQAENRMH 370
           E RR+ F  FQ VN+EL+  A     FMHCLPA RG EVTD VI++PNS+VF QAENR+H
Sbjct: 243 EERRKIFMPFQ-VNKELVKHAKPDYIFMHCLPAHRGEEVTDDVIDSPNSVVFDQAENRLH 302

Query: 371 AQNAIMLHVLG 379
           AQ A+M  V+G
Sbjct: 303 AQKAVMALVMG 311

BLAST of MS008858.1 vs. ExPASy Swiss-Prot
Match: B7GM02 (Ornithine carbamoyltransferase OS=Anoxybacillus flavithermus (strain DSM 21510 / WK1) OX=491915 GN=argF PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.8e-85
Identity = 161/315 (51.11%), Postives = 215/315 (68.25%), Query Frame = 0

Query: 66  SVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAMIFAKP 125
           ++ + ++   KDFL L D+ K  +  +L  AVE+K + ++G+R + P  GK++AMIF KP
Sbjct: 2   NMTATIQLKGKDFLTLADYSKEEIEYLLHLAVELKEKQQNGER-YTPLSGKTLAMIFEKP 61

Query: 126 SMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFAHQDIL 185
           S RTRVSFE G   LGGHA++L   D+Q+G+ E   D ARVLS Y D IM R F H+ + 
Sbjct: 62  STRTRVSFEVGMVQLGGHALHLSSRDLQIGRGETIADTARVLSEYVDAIMIRTFEHEKVE 121

Query: 186 DLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSWLLLAS 245
           +LA YAT+PVINGLTD +HPCQ +AD LTI E  GKL+G+K+ Y+GDGNNM H+ +L A+
Sbjct: 122 ELAHYATIPVINGLTDDDHPCQALADLLTIYEVKGKLQGLKLAYIGDGNNMAHALMLAAA 181

Query: 246 VVPMHFVCACPKGFEPDKNTVEKAQQAGI---SKIEITNDPKEAVKGADVVYSDVWASMG 305
            V MH   A PKG+EP +  V++A+Q      + I +TNDP EA+  ADVVY+DVWASMG
Sbjct: 182 KVGMHCAVASPKGYEPKEAVVKEAKQIANESGATIVVTNDPYEAIADADVVYTDVWASMG 241

Query: 306 QKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFPQA 365
           Q+ EA  R   F  FQ VNE L+  A     F+HCLPA RG EVT+GVI+   S +F QA
Sbjct: 242 QEAEANERMHVFAPFQ-VNEALVQQAKQDFMFLHCLPAHRGEEVTEGVIDGVRSYIFQQA 301

Query: 366 ENRMHAQNAIMLHVL 378
            NR+HAQ A+++ +L
Sbjct: 302 GNRLHAQKALLVSLL 314

BLAST of MS008858.1 vs. ExPASy Swiss-Prot
Match: O58457 (Ornithine carbamoyltransferase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=argF PE=3 SV=3)

HSP 1 Score: 312.4 bits (799), Expect = 7.1e-84
Identity = 161/306 (52.61%), Postives = 214/306 (69.93%), Query Frame = 0

Query: 76  KDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAMIFAKPSMRTRVSFET 135
           +D L L D+    +  IL+ A   K   K G +     +GK++AMIF KPS RTRVSFE 
Sbjct: 8   RDLLCLQDYTPEEIWTILETAKMFKIWQKIG-KPHRLLEGKTLAMIFQKPSTRTRVSFEV 67

Query: 136 GFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFAHQDILDLAKYATVPV 195
               LGGHA+YL   D+Q+ + E   D ARVLSRY D IMARV+AH+D+ DLAKYA+VPV
Sbjct: 68  AMAHLGGHALYLNAQDLQLRRGETIADTARVLSRYVDAIMARVYAHKDVEDLAKYASVPV 127

Query: 196 INGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSWLLLASVVPMHFVCAC 255
           INGL+D++HPCQ +AD +TI E  G ++GVKVVYVGDGNN+ HS ++  + +    V A 
Sbjct: 128 INGLSDFSHPCQALADYMTIWEKKGTIKGVKVVYVGDGNNVCHSLMIAGTKLGADVVVAT 187

Query: 256 PKGFEPDKNTVEKAQQAGI---SKIEITNDPKEAVKGADVVYSDVWASMGQKEEAEYRRQ 315
           P+G+EPDK  ++ A+Q         E+ +DP +AVK ADV+Y+DVWASMGQ+ EAE RR+
Sbjct: 188 PEGYEPDKKVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAEAEERRK 247

Query: 316 AFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFPQAENRMHAQNAI 375
            F+ FQ VN++L+  A S   FMHCLPA RG EVTD VI++PNS+V+ +AENR+HAQ A+
Sbjct: 248 IFRPFQ-VNKDLVKHAKSDYMFMHCLPAHRGEEVTDDVIDSPNSVVWDEAENRLHAQKAV 307

Query: 376 MLHVLG 379
           +  +LG
Sbjct: 308 LALLLG 311

BLAST of MS008858.1 vs. ExPASy TrEMBL
Match: A0A6J1C5E5 (Ornithine carbamoyltransferase OS=Momordica charantia OX=3673 GN=LOC111008625 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 1.9e-212
Identity = 378/379 (99.74%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS
Sbjct: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM
Sbjct: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW
Sbjct: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRRQAFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRQAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. ExPASy TrEMBL
Match: A0A6J1FM42 (Ornithine carbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446653 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.0e-194
Identity = 343/379 (90.50%), Postives = 364/379 (96.04%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+S VRSEK+SL T FYS   +RRSVKF G LSGFSVASPARFPARFP+I+CQTS
Sbjct: 1   MAAICSHSIVRSEKSSLPTHFYSSRGIRRSVKFSGGLSGFSVASPARFPARFPSITCQTS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           AT PS VVSKVKA KKDFLHL+DFDKSTL+KILDRA+EVKAQLKSGDR+F+PF+GK+MAM
Sbjct: 61  ATFPSPVVSKVKAEKKDFLHLSDFDKSTLMKILDRAIEVKAQLKSGDRTFLPFEGKTMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARVF 
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARVFG 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGLTDYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNMVHSW
Sbjct: 181 HQDILDLAKYATVPVINGLTDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMVHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASVVP+HFVCACPKGFEPDKNTVEKA+QAGISKIEI++DPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVVPLHFVCACPKGFEPDKNTVEKARQAGISKIEISHDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRR+AFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRKAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. ExPASy TrEMBL
Match: A0A6J1IY25 (Ornithine carbamoyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480972 PE=3 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 1.9e-193
Identity = 341/379 (89.97%), Postives = 363/379 (95.78%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+S V+SEK++L T FYS  S RRSVKF G LSGFSVAS ARFPARFP+I+CQTS
Sbjct: 1   MAAICSHSIVQSEKSALPTHFYSSRSFRRSVKFSGGLSGFSVASSARFPARFPSITCQTS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           ATSPS VVSKVKA KKDFLHL+DFDKSTL+KILDRA+EVKAQLKSGDR+F+PF+GK+MAM
Sbjct: 61  ATSPSPVVSKVKAEKKDFLHLSDFDKSTLMKILDRAIEVKAQLKSGDRTFLPFEGKTMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARVF 
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARVFG 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGLTDYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNM HSW
Sbjct: 181 HQDILDLAKYATVPVINGLTDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMAHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASVVP+HFVCACPKGFEPDKNTVEKA+QAGISKIEI++DPKEAVKGADVVYSDVWAS
Sbjct: 241 LLLASVVPLHFVCACPKGFEPDKNTVEKARQAGISKIEISHDPKEAVKGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRR+AFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRKAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. ExPASy TrEMBL
Match: A0A6J1FAF0 (Ornithine carbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443546 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.3e-193
Identity = 341/379 (89.97%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+ST+RS+KNSL T FYS  SLRRSVKF GELS FSVASP RFPA FP+ISCQ+S
Sbjct: 1   MAAICSHSTLRSDKNSLPTHFYSARSLRRSVKFFGELSRFSVASPPRFPAGFPSISCQSS 60

Query: 61  ATSPSSVVSKVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAM 120
           AT P SVVS+VKA KKDFLHL+DFDKSTLLKILDRAVEVKA+LKSGDR+F+PFKGK+MAM
Sbjct: 61  ATYPPSVVSQVKAEKKDFLHLSDFDKSTLLKILDRAVEVKARLKSGDRTFLPFKGKTMAM 120

Query: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARVFA 180
           IFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARVF 
Sbjct: 121 IFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARVFG 180

Query: 181 HQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVHSW 240
           HQDILDLAKYATVPVINGL+DYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNMVHSW
Sbjct: 181 HQDILDLAKYATVPVINGLSDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMVHSW 240

Query: 241 LLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWAS 300
           LLLASV+P HFVCACPKGFEPDK+TVEKA+QAG+SKIEITNDPKEAV+GADVVYSDVWAS
Sbjct: 241 LLLASVIPFHFVCACPKGFEPDKHTVEKARQAGVSKIEITNDPKEAVRGADVVYSDVWAS 300

Query: 301 MGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFP 360
           MGQKEEAEYRRQAFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTD VIEAPNSIVFP
Sbjct: 301 MGQKEEAEYRRQAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDEVIEAPNSIVFP 360

Query: 361 QAENRMHAQNAIMLHVLGL 380
           QAENRMHAQNAIMLHVLGL
Sbjct: 361 QAENRMHAQNAIMLHVLGL 378

BLAST of MS008858.1 vs. ExPASy TrEMBL
Match: A0A6J1FKS8 (Ornithine carbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446653 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 7.4e-193
Identity = 342/381 (89.76%), Postives = 364/381 (95.54%), Query Frame = 0

Query: 1   MAAICSYSTVRSEKNSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISCQTS 60
           MAAICS+S VRSEK+SL T FYS   +RRSVKF G LSGFSVASPARFPARFP+I+CQTS
Sbjct: 1   MAAICSHSIVRSEKSSLPTHFYSSRGIRRSVKFSGGLSGFSVASPARFPARFPSITCQTS 60

Query: 61  ATSPSSVVSK--VKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSM 120
           AT PS VVSK  +KA KKDFLHL+DFDKSTL+KILDRA+EVKAQLKSGDR+F+PF+GK+M
Sbjct: 61  ATFPSPVVSKGVLKAEKKDFLHLSDFDKSTLMKILDRAIEVKAQLKSGDRTFLPFEGKTM 120

Query: 121 AMIFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIMARV 180
           AMIFAKPSMRTRVSFETGFFLLGGHAIYLGP+ IQMGKREETRD+ARVLSRYNDVIMARV
Sbjct: 121 AMIFAKPSMRTRVSFETGFFLLGGHAIYLGPDTIQMGKREETRDIARVLSRYNDVIMARV 180

Query: 181 FAHQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNNMVH 240
           F HQDILDLAKYATVPVINGLTDYNHPCQ+MADALT+IEHIGKLEG KVVYVGDGNNMVH
Sbjct: 181 FGHQDILDLAKYATVPVINGLTDYNHPCQVMADALTMIEHIGKLEGTKVVYVGDGNNMVH 240

Query: 241 SWLLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVW 300
           SWLLLASVVP+HFVCACPKGFEPDKNTVEKA+QAGISKIEI++DPKEAVKGADVVYSDVW
Sbjct: 241 SWLLLASVVPLHFVCACPKGFEPDKNTVEKARQAGISKIEISHDPKEAVKGADVVYSDVW 300

Query: 301 ASMGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIV 360
           ASMGQKEEAEYRR+AFQGFQ VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIV
Sbjct: 301 ASMGQKEEAEYRRKAFQGFQ-VNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIV 360

Query: 361 FPQAENRMHAQNAIMLHVLGL 380
           FPQAENRMHAQNAIMLHVLGL
Sbjct: 361 FPQAENRMHAQNAIMLHVLGL 380

BLAST of MS008858.1 vs. TAIR 10
Match: AT1G75330.1 (ornithine carbamoyltransferase )

HSP 1 Score: 543.1 bits (1398), Expect = 1.8e-154
Identity = 283/383 (73.89%), Postives = 324/383 (84.60%), Query Frame = 0

Query: 2   AAICSY-STVRSEK---NSLTTQFYSGDSLRRSVKFLGELSGFSVASPARFPARFPAISC 61
           AA+ S+ ST RS     +S ++ F+ G +LRR        S  S+ SPA    R   +SC
Sbjct: 3   AAMASHVSTARSPALSFSSSSSSFFPGTTLRR-------FSAVSLPSPALPRLR---VSC 62

Query: 62  Q-TSATSPSSVVS-KVKAGKKDFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKG 121
           Q +S TSPSS    K K+  KDFL ++DFD +T+  ILD+A EVKA LKSG+R+++PFKG
Sbjct: 63  QASSVTSPSSPSDVKGKSDLKDFLAIDDFDTATIKTILDKASEVKALLKSGERNYLPFKG 122

Query: 122 KSMAMIFAKPSMRTRVSFETGFFLLGGHAIYLGPNDIQMGKREETRDVARVLSRYNDVIM 181
           KSM+MIFAKPSMRTRVSFETGFFLLGGHA+YLGPNDIQMGKREETRDVARVLSRYND+IM
Sbjct: 123 KSMSMIFAKPSMRTRVSFETGFFLLGGHALYLGPNDIQMGKREETRDVARVLSRYNDIIM 182

Query: 182 ARVFAHQDILDLAKYATVPVINGLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGDGNN 241
           ARVFAHQDILDLA Y++VPV+NGLTD+NHPCQ+MADALT+IEHIG++EG KVVYVGDGNN
Sbjct: 183 ARVFAHQDILDLANYSSVPVVNGLTDHNHPCQIMADALTMIEHIGQVEGTKVVYVGDGNN 242

Query: 242 MVHSWLLLASVVPMHFVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYS 301
           MVHSWL LASV+P HFVCACPKG+EPDK  V KA+QAG+SKIEITNDPKEAV GADVVYS
Sbjct: 243 MVHSWLELASVIPFHFVCACPKGYEPDKERVSKAKQAGLSKIEITNDPKEAVIGADVVYS 302

Query: 302 DVWASMGQKEEAEYRRQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPN 361
           DVWASMGQK+EAE RR+AFQGFQ V+E LM LAG KAYFMHCLPAERGVEVT+GV+EAP 
Sbjct: 303 DVWASMGQKDEAEARRKAFQGFQ-VDEALMKLAGQKAYFMHCLPAERGVEVTNGVVEAPY 362

Query: 362 SIVFPQAENRMHAQNAIMLHVLG 379
           SIVFPQAENRMHAQNAIMLH+LG
Sbjct: 363 SIVFPQAENRMHAQNAIMLHLLG 374

BLAST of MS008858.1 vs. TAIR 10
Match: AT3G20330.1 (PYRIMIDINE B )

HSP 1 Score: 112.1 bits (279), Expect = 1.0e-24
Identity = 94/306 (30.72%), Postives = 141/306 (46.08%), Query Frame = 0

Query: 77  DFLHLNDFDKSTLLKILDRAVEVKAQLKSGDRSFVPFKGKSMAMIFAKPSMRTRVSFETG 136
           D +    FD+  L  I D A E++   KS  +S +  KG  MA +F +PS RTR+SFE+ 
Sbjct: 86  DVIEGKQFDREMLSAIFDVAREMEKIEKSSSQSEI-LKGYLMATLFYEPSTRTRLSFESA 145

Query: 137 FFLLGGHAIYL--GPNDIQMGKREETRDVARVLSRYNDVIMARVFAHQDILDLAKYATVP 196
              LGG  +            K E   D  R +  Y+D+I+ R F        A  A +P
Sbjct: 146 MKRLGGEVLTTENAREFSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARKAAATANIP 205

Query: 197 VIN-GLTDYNHPCQLMADALTIIEHIGKLEGVKVVYVGD---GNNMVHSWLLLASVVPMH 256
           VIN G     HP Q + D  TI   IGKL+G+ V  VGD   G  +     LLA    + 
Sbjct: 206 VINAGDGPGEHPTQALLDVYTIQSEIGKLDGISVALVGDLANGRTVRSLAYLLAKFKDVK 265

Query: 257 FVCACPKGFEPDKNTVEKAQQAGISKIEITNDPKEAVKGADVVYSDVWASMGQKEEAEYR 316
                P+  +   +  +    +G+ + E ++D  E     DVVY     +  Q+E    R
Sbjct: 266 IYFVSPEIVKMKDDIKDYLTSSGV-EWEESSDLMEVASKCDVVYQ----TRIQRERFGER 325

Query: 317 ---RQAFQGFQVVNEELMNLAGSKAYFMHCLPAERGVEVTDGVIEAPNSIVFPQAENRMH 374
               +A +G  +V+++L+ +   KA  MH LP  R  E+T  V   P +  F QA+N + 
Sbjct: 326 LDLYEAARGKYIVDKDLLGVMQKKAIIMHPLP--RLDEITADVDADPRAAYFRQAKNGLF 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137066.13.9e-21299.74ornithine carbamoyltransferase, chloroplastic [Momordica charantia][more]
XP_038899565.11.9e-19590.77ornithine carbamoyltransferase, chloroplastic [Benincasa hispida][more]
XP_022941317.12.1e-19490.50ornithine carbamoyltransferase, chloroplastic-like isoform X2 [Cucurbita moschat... [more]
XP_023536248.14.0e-19389.97ornithine carbamoyltransferase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022981986.14.0e-19389.97ornithine carbamoyltransferase, chloroplastic-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q438142.9e-15475.13Ornithine carbamoyltransferase, chloroplastic OS=Pisum sativum OX=3888 GN=ARGF P... [more]
O500392.5e-15373.89Ornithine transcarbamylase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OTC... [more]
Q5JI162.9e-8552.41Ornithine carbamoyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918... [more]
B7GM023.8e-8551.11Ornithine carbamoyltransferase OS=Anoxybacillus flavithermus (strain DSM 21510 /... [more]
O584577.1e-8452.61Ornithine carbamoyltransferase OS=Pyrococcus horikoshii (strain ATCC 700860 / DS... [more]
Match NameE-valueIdentityDescription
A0A6J1C5E51.9e-21299.74Ornithine carbamoyltransferase OS=Momordica charantia OX=3673 GN=LOC111008625 PE... [more]
A0A6J1FM421.0e-19490.50Ornithine carbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446653 PE=... [more]
A0A6J1IY251.9e-19389.97Ornithine carbamoyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480972 PE=3 ... [more]
A0A6J1FAF03.3e-19389.97Ornithine carbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443546 PE=... [more]
A0A6J1FKS87.4e-19389.76Ornithine carbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446653 PE=... [more]
Match NameE-valueIdentityDescription
AT1G75330.11.8e-15473.89ornithine carbamoyltransferase [more]
AT3G20330.11.0e-2430.72PYRIMIDINE B [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006130Aspartate/ornithine carbamoyltransferasePRINTSPR00100AOTCASEcoord: 204..215
score: 57.14
coord: 342..365
score: 44.32
coord: 122..141
score: 53.96
coord: 330..339
score: 50.91
IPR002292Ornithine/putrescine carbamoyltransferasePRINTSPR00102OTCASEcoord: 153..166
score: 46.84
coord: 293..303
score: 65.12
coord: 356..367
score: 67.44
coord: 120..134
score: 67.13
coord: 193..207
score: 70.23
IPR002292Ornithine/putrescine carbamoyltransferaseTIGRFAMTIGR00658TIGR00658coord: 76..377
e-value: 8.0E-117
score: 387.7
IPR006131Aspartate/ornithine carbamoyltransferase, Asp/Orn-binding domainPFAMPF00185OTCacecoord: 223..375
e-value: 7.9E-56
score: 188.4
IPR036901Aspartate/ornithine carbamoyltransferase superfamilyGENE3D3.40.50.1370Aspartate/ornithine carbamoyltransferasecoord: 207..373
e-value: 1.4E-118
score: 397.8
IPR036901Aspartate/ornithine carbamoyltransferase superfamilyGENE3D3.40.50.1370Aspartate/ornithine carbamoyltransferasecoord: 76..206
e-value: 1.4E-118
score: 397.8
IPR036901Aspartate/ornithine carbamoyltransferase superfamilySUPERFAMILY53671Aspartate/ornithine carbamoyltransferasecoord: 75..378
IPR006132Aspartate/ornithine carbamoyltransferase, carbamoyl-P bindingPFAMPF02729OTCace_Ncoord: 76..217
e-value: 1.4E-46
score: 158.2
NoneNo IPR availablePANTHERPTHR45753:SF5ORNITHINE CARBAMOYLTRANSFERASE, CHLOROPLASTIC-LIKEcoord: 1..379
NoneNo IPR availablePANTHERPTHR45753ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIALcoord: 1..379
IPR024904Ornithine carbamoyltransferaseHAMAPMF_01109OTCasecoord: 75..378
score: 73.05529

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS008858MS008858gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS008858.1-cdsMS008858.1-cds-scaffold4:4312532..4312711CDS
MS008858.1-cdsMS008858.1-cds-scaffold4:4313470..4313748CDS
MS008858.1-cdsMS008858.1-cds-scaffold4:4313848..4313979CDS
MS008858.1-cdsMS008858.1-cds-scaffold4:4314770..4315104CDS
MS008858.1-cdsMS008858.1-cds-scaffold4:4315580..4315790CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS008858.1MS008858.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
biological_process GO:0006591 ornithine metabolic process
molecular_function GO:0016597 amino acid binding
molecular_function GO:0016743 carboxyl- or carbamoyltransferase activity
molecular_function GO:0004585 ornithine carbamoyltransferase activity