MS006363.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS006363.1 vs. NCBI nr
Match: XP_038887470.1 (clathrin coat assembly protein AP180 [Benincasa hispida])

HSP 1 Score: 857.8 bits (2215), Expect = 5.7e-245
Identity = 469/633 (74.09%), Postives = 506/633 (79.94%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGA-NPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSI LAKVSS A  P AT+LEVAVLKATTHDD PLD+RYVSEIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDGPLDHRYVSEILQ 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS A ACA +IAKRITKTRNWIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSNAAACAHSIAKRITKTRNWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK  S+HG    R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKIESYHG--TTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI++WQKLLDRAIATRPTGPAK NRLV ++LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           I+DGLALLLDSFFHLQYQSCVNAFQACVKA KQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ITDGLALLLDSFFHLQYQSCVNAFQACVKATKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCHG+RSPPPQ  LP      + L+DDLEQSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHGSRSPPPQAILPGP---TTKLTDDLEQSESSDKNSERG 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS+GDIMSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 SDLNSVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NESNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFGAPSGRLGDWELVLAESATEPPPQEWPDFFAPSIGDDEFV 480
           AFF    +EN  P+QE      PS R GDWELVLAESAT   PQEWPDFF+PSIGDD F 
Sbjct: 421 AFF----EENGTPFQEPQFGATPSVRFGDWELVLAESAT-GSPQEWPDFFSPSIGDDLFA 480

Query: 481 KSISPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAPTF 540
           K  SPQ  YNNPFLQ +DDL AVPPTF A     E EIAPTFRAQN KE    V+VAPTF
Sbjct: 481 KPFSPQHHYNNPFLQDSDDLLAVPPTFKA-----EREIAPTFRAQNSKE---TVSVAPTF 540

Query: 541 RAETAPFCPANNLN--FEAWGSTETVVANSTKESNLFSFNPAG-EDDPFAVSWRSSEKGN 600
           RAETA     N  +  FEAWG+ +            F F+  G E+DPFA  W  +  GN
Sbjct: 541 RAETATLSSKNQNSSMFEAWGTVQ--------NGRGFGFDDLGVENDPFAGPWSRNLNGN 596

Query: 601 DRSFDGKTMNEESLLEQQKLWKEQQNKIIAKHL 622
           D +FD     +ESL++QQKLWKEQQNKIIAKHL
Sbjct: 601 DPNFD-----QESLMQQQKLWKEQQNKIIAKHL 596

BLAST of MS006363.1 vs. NCBI nr
Match: XP_022969464.1 (clathrin coat assembly protein AP180-like [Cucurbita maxima])

HSP 1 Score: 836.6 bits (2160), Expect = 1.4e-238
Identity = 459/633 (72.51%), Postives = 505/633 (79.78%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSG-ANPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSISLAKVSS    P A +LEVA+LKATTHDDVPL+ RYV EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVVEILH 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A  CA +IAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S +G    R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFNG--NTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI +WQKLLDRAIATRPTGPAK NRLVL +LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCH +RSPP  V L    S    L+DDLEQSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMK-LTDDLEQSESSDKNSERE 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS G++MSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 LDFNSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NESNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFGAPSG-RLGDWELVLAESATEPPPQEWPDFFAPSIGDDEF 480
           AFF+D    + KP+QE+  FGA  G R GDWELVLAESAT+   +EWPDFFAPSIGDD F
Sbjct: 421 AFFED----DEKPFQEESEFGAAPGLRCGDWELVLAESATD-TAEEWPDFFAPSIGDDPF 480

Query: 481 VKSI-SPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
            K   SPQ QYNNPFLQ +++ FAVPPTF A     ETEIAPTFRA+N KE   AV VAP
Sbjct: 481 EKPFSSPQHQYNNPFLQDSNESFAVPPTFKA-----ETEIAPTFRAENVKE---AVVVAP 540

Query: 541 TFRAETAPFCP--ANNLNFEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 600
           TFR ET  F     N   F+ WG     + N +   + F F+   E+DPFA +W S+  G
Sbjct: 541 TFRVETPTFSSQHQNGSMFDDWGP----IPNGSGSGSGFEFDLGAENDPFAEAWNSNLNG 600

Query: 601 NDRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           N+ S +   M++ESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 NEPSLN--VMDQESLLQQQKLWKEQQNKIIAKH 605

BLAST of MS006363.1 vs. NCBI nr
Match: XP_023553928.1 (clathrin coat assembly protein AP180-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 835.5 bits (2157), Expect = 3.0e-238
Identity = 460/633 (72.67%), Postives = 504/633 (79.62%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSG-ANPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSISLAKVSS    P A +LEVA+LKATTHDDVPL+ RYV+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVAEILQ 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A  CA +IAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S HG    R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHG--NTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI +WQKLLDRAIATRPTGPAK NRLVL +LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCH +RSPP  V L    S    L+DDLEQSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMK-LTDDLEQSESSDKNSERE 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS G++MSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 LDFNSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NESNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFGAPSG-RLGDWELVLAESATEPPPQEWPDFFAPSIGDDEF 480
           AFFDD    +  P+QE+  FGA  G R GDWELVLAESAT+   +EWPDFFAPSIGDD F
Sbjct: 421 AFFDD----DETPFQEESEFGATPGLRCGDWELVLAESATD-TAEEWPDFFAPSIGDDPF 480

Query: 481 VKSI-SPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
            K   SPQ QYNNPFLQ +++ FAVPPTF A     ETEIAPTFRA+N KE   AV VAP
Sbjct: 481 EKPFTSPQHQYNNPFLQDSNESFAVPPTFKA-----ETEIAPTFRAENVKE---AVVVAP 540

Query: 541 TFRAETAPFCPANNLN--FEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 600
           TFR ETA F   N     F+ WG     + N +     F F+   E+DPFA +W S+  G
Sbjct: 541 TFRVETATFSSQNQNGSMFDDWGP----IPNGSGSG--FEFDLGAENDPFAEAWDSNLNG 600

Query: 601 NDRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           N+ S +   M++ESLL+QQKLWKE QNKIIAKH
Sbjct: 601 NEPSLN--VMDQESLLQQQKLWKEHQNKIIAKH 603

BLAST of MS006363.1 vs. NCBI nr
Match: XP_022952666.1 (clathrin coat assembly protein AP180 [Cucurbita moschata])

HSP 1 Score: 833.2 bits (2151), Expect = 1.5e-237
Identity = 459/633 (72.51%), Postives = 504/633 (79.62%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSG-ANPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSISLAKVSS    P A +LEVA+LKATTHDDVPL+ RYV+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVAEILQ 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A  CA +IAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S HG    R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHG--NTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI +WQKLLDRAIATRPTGPAK NRLVL +LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCH +RSPP  V L    S    L+DDLEQSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMK-LTDDLEQSESSDKNSERE 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS G++MSATGSW SP NSVEQ+GE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 LDFNSFGEMMSATGSWTSPANSVEQEGEGYSDYQSEKQSRLGDFA------NESNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFGAPSG-RLGDWELVLAESATEPPPQEWPDFFAPSIGDDEF 480
           AFF+D    +  P+QE+   GA  G R GDWELVLAESAT+   +EWPDFFAPSIGDD F
Sbjct: 421 AFFED----DETPFQEETELGAARGLRCGDWELVLAESATD-TAEEWPDFFAPSIGDDPF 480

Query: 481 VKSI-SPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
            K   SPQ QYNNPFLQ +++ FAVPPTF A     ETEIAPTFRA+N KE   AVAVAP
Sbjct: 481 EKPFTSPQHQYNNPFLQDSNESFAVPPTFKA-----ETEIAPTFRAENVKE---AVAVAP 540

Query: 541 TFRAETAPFCPANNLN--FEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 600
           TFR ET  F   N     F+ WG     + N +     F F+   E+DPFA +W SS  G
Sbjct: 541 TFRVETPTFSSQNQNGSMFDDWGP----IPNGSGSG--FEFDLGAENDPFAEAWDSSLNG 600

Query: 601 NDRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           N+ S +   M++ESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 NEPSLN--VMDQESLLQQQKLWKEQQNKIIAKH 603

BLAST of MS006363.1 vs. NCBI nr
Match: KAG6572416.1 (Clathrin coat assembly protein 180, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012015.1 Clathrin coat assembly protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.0 bits (2148), Expect = 3.3e-237
Identity = 458/633 (72.35%), Postives = 503/633 (79.46%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSG-ANPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSISLAKVSS    P A +LEVA+LKATTHDDVPL+ RYV+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVAEILQ 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A  CA +IAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S HG    R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHG--NTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI +WQKLLDRAIATRPTGPAK NRLVL +LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCH +RSPP  V L    S    L+DDLEQSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMK-LTDDLEQSESSDKNSERE 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS G++MSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 LDFNSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NESNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFGAPSG-RLGDWELVLAESATEPPPQEWPDFFAPSIGDDEF 480
           AFF+D    +  P+QE+   GA  G R GDWELVLAESAT+   +EWPDFFAPSIGDD F
Sbjct: 421 AFFED----DETPFQEETELGAARGLRCGDWELVLAESATD-TAEEWPDFFAPSIGDDPF 480

Query: 481 VKSI-SPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
            K   SPQ QYNNPFLQ +++ FAVPPTF A     ETEIAPTFRA+N KE   AV VAP
Sbjct: 481 EKPFTSPQHQYNNPFLQDSNESFAVPPTFKA-----ETEIAPTFRAENVKE---AVVVAP 540

Query: 541 TFRAETAPFCPANNLN--FEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 600
           TFR ET  F   N     F+ WG     + N +     F F+   E+DPFA +W S+  G
Sbjct: 541 TFRVETPAFSSQNQNGSMFDDWGP----IPNGSGSG--FEFDLGAENDPFAEAWDSNLNG 600

Query: 601 NDRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           N+ S +   M++ESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 NEPSLN--VMDQESLLQQQKLWKEQQNKIIAKH 603

BLAST of MS006363.1 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 6.3e-138
Identity = 324/695 (46.62%), Postives = 411/695 (59.14%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGANPAA--TTLEVAVLKATTHD-DVPLDNRYVSEI 60
           MPSK +KAI AVKDQTSISLAKV++GA      TTLEVA+LKAT+HD +VP+D+R V+EI
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEI 60

Query: 61  LSLIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRG 120
           L +I+S KS+A +CA+AI +RI +TRNWIVALKSL LVLRIFQDGDPYFPREVLHAMKRG
Sbjct: 61  LGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRG 120

Query: 121 AKILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRK--------- 180
           AKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFLTGKLQ+R+TNR+         
Sbjct: 121 AKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNS 180

Query: 181 -SHSHHGPPPNRRINEP-IRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTL 240
            + S   P    + +EP +RDMKP ML+D+I++WQKLLDRAIATRPTG AK+NRLV  +L
Sbjct: 181 TTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSL 240

Query: 241 HAVVHESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNI 300
           +AV+ ESFDLYRDISDGLALLLDSFFHLQYQSC+NAFQACV+A+KQFEEL +FYDL K+I
Sbjct: 241 YAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSI 300

Query: 301 GVGRTSEYPSVQQPSDELIETLQEFLKDQASFPCHGNRSPPPQVFLP----AKDSIPSIL 360
           G+GRTSEYPS+Q+ S EL+ETLQEFLKDQ+SFP      P P  FLP    +KDS  S  
Sbjct: 301 GIGRTSEYPSIQKISLELLETLQEFLKDQSSFPASSGLYPSPNSFLPPPPSSKDSAVSSS 360

Query: 361 SD----DLEQSE------SSDRNSMGDIMSAT-GSWASPTNSVEQDGESYSDYQSEKQSQ 420
            D     ++ SE      S    S+ D+MS T    +SP  S     E Y   + +    
Sbjct: 361 LDFGDSTIDTSERYSDYGSFRSTSLEDLMSRTEAGTSSPPMSCH--SEPYGGGRDDPNGN 420

Query: 421 FGDSASMNGNENEQNLTPNFAFFD------------------------------------ 480
             D+ S     N  +++ +    D                                    
Sbjct: 421 NFDTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKAETFDPWEAL 480

Query: 481 ---DGPQENVKPYQEQLHFGAPSGR-LGDWELVLAESATEPPPQEWPDFFAPSIGDDEFV 540
              D P++ ++  +E+        R  G+W L L E+AT+   Q          G D+ +
Sbjct: 481 MLRDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQ--VQGNNSMAIVPFGLDDPM 540

Query: 541 KSISPQQQYNNPFLQG--ADDLFAVPPTFT-AEFSSPETEIAPTFRAQNFKEETVAVAVA 600
            +        NPFL+   A    A  P  T    +    +  PTF+      +    +  
Sbjct: 541 PAFQAATDQYNPFLEEPVAQLATAGEPMITFGGLALTGFQPEPTFQVN--VPDDFEPSST 600

Query: 601 PTFRA-ETAPFCPANNLNFEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 623
           PTF+A ET P        FE++G  ET                             SE G
Sbjct: 601 PTFKATETLPMKCDPFTTFESFGFGETF----------------------------SENG 653

BLAST of MS006363.1 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 291.2 bits (744), Expect = 2.8e-77
Identity = 160/363 (44.08%), Postives = 228/363 (62.81%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSL 60
           M    RKAI  VKDQTSI +AKV+S   P    LEVA++KAT+HDD    ++Y+ EILSL
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASNMAP---DLEVAIVKATSHDDDQSSDKYIREILSL 60

Query: 61  IASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 120
            + S+ Y  AC +++++R+ KTR+WIVALK+L LV R+  +GDP F  E+L+A +RG +I
Sbjct: 61  TSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRI 120

Query: 121 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTN----RKSHSHHG-- 180
           LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L    ++R  N      SH  +G  
Sbjct: 121 LNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELAL---FERRGRNGGGSSSSHQSNGDD 180

Query: 181 -----------PPPNR-------------------RINE-----------PIRDMKPAML 240
                      PPP                      +NE           P+R+M P  +
Sbjct: 181 GYNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERI 240

Query: 241 IDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRDISDGLALLLDSFFH 300
             ++ H Q+LLDR ++ RPTG AK++R++L  ++ VV ESF LY DI + LA+LLD FF 
Sbjct: 241 FGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFD 300

Query: 301 LQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQPSDELIETLQEFLK 317
           ++Y  CV AF A   AAKQ +EL +FY  CK+ GV R+SEYP VQ+ + +L+ETL+EF++
Sbjct: 301 MEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVR 357

BLAST of MS006363.1 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 290.0 bits (741), Expect = 6.2e-77
Identity = 160/398 (40.20%), Postives = 233/398 (58.54%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSL 60
           M    RKAI AVKDQTSI +AKV+S   P    LEVA++KAT+HDD P   +Y+ EIL+L
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASNMAP---DLEVAIVKATSHDDDPASEKYIREILNL 60

Query: 61  IASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 120
            + S+ Y  AC +++++R++KTR+W+VALK+L LV R+  +GDP F  E+L++ +RG ++
Sbjct: 61  TSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRM 120

Query: 121 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHH------- 180
           LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L  +      N   +S H       
Sbjct: 121 LNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDR 180

Query: 181 ---------GPPP----------------------------------------------- 240
                     PPP                                               
Sbjct: 181 YGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGR 240

Query: 241 -NRRINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDL 300
             +++  P+R+M P  +  ++ H Q+LLDR ++ RPTG AK++R++L  L+ VV ESF L
Sbjct: 241 DEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKL 300

Query: 301 YRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPS 331
           Y DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEYP 
Sbjct: 301 YADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPE 360

BLAST of MS006363.1 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.2e-73
Identity = 163/466 (34.98%), Postives = 266/466 (57.08%), Query Frame = 0

Query: 3   SKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSLIA 62
           SKF++AI AVKDQTS+ LAKV +G + + + L+VA++KAT H++ P + +Y+ EILSL +
Sbjct: 4   SKFKRAIGAVKDQTSVGLAKV-NGRSASLSELDVAIVKATRHEEFPAEEKYIREILSLTS 63

Query: 63  SSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKILN 122
            S+SY  AC S +++R+ KT+ W VALK+L L+ R+  +GD  + +E+  A +RG ++LN
Sbjct: 64  YSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRLLN 123

Query: 123 LSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKR-------FTNRKSHSHHGP 182
           +S+FRD S S+ WDY+AFVRT+ALYLDERLD  +  +  KR         + +       
Sbjct: 124 MSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQAAA 183

Query: 183 PPNRRI---NEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHE 242
             +  I   ++PI +MK   +  RI H Q+LLDR +A RPTG A++NR+V+  L+ +V E
Sbjct: 184 DLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKE 243

Query: 243 SFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTS 302
           SF +Y D+++ + +L++ F  L     +  +    + +KQFEEL  FY  CKN+G+ R+S
Sbjct: 244 SFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSS 303

Query: 303 EYPSVQQPSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESS 362
           EYP +++ + + ++ + EF++D+++   H  +S           S+ S   +D +++ + 
Sbjct: 304 EYPEIEKITQKKLDLMDEFIRDKSALE-HTKQS----------KSVKSEADEDDDEARTE 363

Query: 363 DRNSMGDIMSATGSWASPTNSVEQDGESYSDYQSE-----KQSQFGDSASM---NGNENE 422
           + N   + M+A  +   P    E D +   + + E     KQ + GD   +   NG E  
Sbjct: 364 EVNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAG 423

Query: 423 QNLTPNFAFFDDGPQENVKPYQEQLHFGAPSGRLGDWELVLAESAT 451
           Q          DGP  +    +    + A      DWE  L ++AT
Sbjct: 424 QAGDSLALALFDGPYASGSGSESGPGWEAFKDDSADWETALVQTAT 457

BLAST of MS006363.1 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 1.1e-70
Identity = 163/474 (34.39%), Postives = 263/474 (55.49%), Query Frame = 0

Query: 3   SKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSLIA 62
           SK ++AI AVKDQTS+ LAKV  G + + T LE+AV+KAT HDD P +++Y+ EIL L +
Sbjct: 4   SKLKRAIGAVKDQTSVGLAKV-GGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCLTS 63

Query: 63  SSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKILN 122
            S++Y  AC + +++R+ KT+NW VALK+L L+ R+  DGD  + +E+  A +RG ++LN
Sbjct: 64  YSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRLLN 123

Query: 123 LSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT---------NRKSHSHH 182
           +S+FRD S S  WDY+AFVRT+ALYLDERLD  + G+  K+ +         + +   H 
Sbjct: 124 MSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDDHR 183

Query: 183 GPPPNRRI------NEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLH 242
           G   + R       ++P+ +MK   + +R+ H Q+LLDR +A RPTG AK+NR+V+  ++
Sbjct: 184 GTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAMY 243

Query: 243 AVVHESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIG 302
            +V ESF LY +I++ + +L++ F  L     +  ++   + +KQF+EL  FY  CKN+ 
Sbjct: 244 PIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMA 303

Query: 303 VGRTSEYPSVQQPSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLE 362
           V R+SEYP +++ + + ++ + EF++D+++      +S        +K S  S   +   
Sbjct: 304 VARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKS-------SSKRSNKSEEEESKT 363

Query: 363 QSESSDRNSMGDIMSATGSWASPTNSVEQDGESYSDYQS--EKQSQFGDSASMNGNENEQ 422
           +    ++  +  I +            E+  E+  D +    +Q Q GD   +    +E 
Sbjct: 364 EYIQENQEDLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDLLDLT---DEA 423

Query: 423 NLTPN-------FAFFDD--GPQENVKPYQEQLHFGAPSGRLGDWELVLAESAT 451
            +T          A FD   G +    P  E     A +    DWE  L  SAT
Sbjct: 424 GVTAGTVGDSLALALFDGVVGTESASGPGWE-----AFNDNSADWETDLVRSAT 461

BLAST of MS006363.1 vs. ExPASy TrEMBL
Match: A0A6J1I2N8 (clathrin coat assembly protein AP180-like OS=Cucurbita maxima OX=3661 GN=LOC111468453 PE=4 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 6.6e-239
Identity = 459/633 (72.51%), Postives = 505/633 (79.78%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSG-ANPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSISLAKVSS    P A +LEVA+LKATTHDDVPL+ RYV EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVVEILH 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A  CA +IAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S +G    R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFNG--NTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI +WQKLLDRAIATRPTGPAK NRLVL +LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCH +RSPP  V L    S    L+DDLEQSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMK-LTDDLEQSESSDKNSERE 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS G++MSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 LDFNSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NESNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFGAPSG-RLGDWELVLAESATEPPPQEWPDFFAPSIGDDEF 480
           AFF+D    + KP+QE+  FGA  G R GDWELVLAESAT+   +EWPDFFAPSIGDD F
Sbjct: 421 AFFED----DEKPFQEESEFGAAPGLRCGDWELVLAESATD-TAEEWPDFFAPSIGDDPF 480

Query: 481 VKSI-SPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
            K   SPQ QYNNPFLQ +++ FAVPPTF A     ETEIAPTFRA+N KE   AV VAP
Sbjct: 481 EKPFSSPQHQYNNPFLQDSNESFAVPPTFKA-----ETEIAPTFRAENVKE---AVVVAP 540

Query: 541 TFRAETAPFCP--ANNLNFEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 600
           TFR ET  F     N   F+ WG     + N +   + F F+   E+DPFA +W S+  G
Sbjct: 541 TFRVETPTFSSQHQNGSMFDDWGP----IPNGSGSGSGFEFDLGAENDPFAEAWNSNLNG 600

Query: 601 NDRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           N+ S +   M++ESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 NEPSLN--VMDQESLLQQQKLWKEQQNKIIAKH 605

BLAST of MS006363.1 vs. ExPASy TrEMBL
Match: A0A6J1GKV6 (clathrin coat assembly protein AP180 OS=Cucurbita moschata OX=3662 GN=LOC111455292 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 7.3e-238
Identity = 459/633 (72.51%), Postives = 504/633 (79.62%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSG-ANPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSISLAKVSS    P A +LEVA+LKATTHDDVPL+ RYV+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVAEILQ 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A  CA +IAKRI KTR+WIVALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S HG    R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHG--NTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI +WQKLLDRAIATRPTGPAK NRLVL +LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCH +RSPP  V L    S    L+DDLEQSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMK-LTDDLEQSESSDKNSERE 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS G++MSATGSW SP NSVEQ+GE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 LDFNSFGEMMSATGSWTSPANSVEQEGEGYSDYQSEKQSRLGDFA------NESNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFGAPSG-RLGDWELVLAESATEPPPQEWPDFFAPSIGDDEF 480
           AFF+D    +  P+QE+   GA  G R GDWELVLAESAT+   +EWPDFFAPSIGDD F
Sbjct: 421 AFFED----DETPFQEETELGAARGLRCGDWELVLAESATD-TAEEWPDFFAPSIGDDPF 480

Query: 481 VKSI-SPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
            K   SPQ QYNNPFLQ +++ FAVPPTF A     ETEIAPTFRA+N KE   AVAVAP
Sbjct: 481 EKPFTSPQHQYNNPFLQDSNESFAVPPTFKA-----ETEIAPTFRAENVKE---AVAVAP 540

Query: 541 TFRAETAPFCPANNLN--FEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 600
           TFR ET  F   N     F+ WG     + N +     F F+   E+DPFA +W SS  G
Sbjct: 541 TFRVETPTFSSQNQNGSMFDDWGP----IPNGSGSG--FEFDLGAENDPFAEAWDSSLNG 600

Query: 601 NDRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           N+ S +   M++ESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 NEPSLN--VMDQESLLQQQKLWKEQQNKIIAKH 603

BLAST of MS006363.1 vs. ExPASy TrEMBL
Match: A0A5D3C685 (Clathrin coat assembly protein AP180 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00110 PE=4 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 1.3e-234
Identity = 456/632 (72.15%), Postives = 496/632 (78.48%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGA-NPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSI LAKVSS A  P AT+LEVAVLKATTHDDVPLD+RYV+EIL+
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDVPLDHRYVNEILT 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A ACA +IAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S+H     R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHA--TTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI++WQKLLDRAIATRPTGPAK NRLV ++LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIP-SILSDDLEQSESSDR---- 360
           PSDELIETLQEFLKDQASFPCHGNRSPP  +      S+P + L+DDLEQSESSD+    
Sbjct: 301 PSDELIETLQEFLKDQASFPCHGNRSPPQAIL-----SVPMTKLTDDLEQSESSDKNSER 360

Query: 361 ----NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPN 420
               NS+GDIMSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PN
Sbjct: 361 GSDFNSVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NESNLSPN 420

Query: 421 FAFFDDGPQENVKPYQEQLHFG-APSGRLGDWELVLAESATEPPPQEWPDFFAPSIGDDE 480
           FAFF    +EN  P++E+  FG APS R GDWELVLAESAT   P+EWPDFF+PSIGDD 
Sbjct: 421 FAFF----EENGMPFEEESQFGVAPSLRFGDWELVLAESAT-ASPKEWPDFFSPSIGDDL 480

Query: 481 FVKSISPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
           F K  SPQ  Y NPFLQ +DDLF  PPTF A     E E+ PTFRA+N        +VAP
Sbjct: 481 FAKPFSPQHHYKNPFLQDSDDLFTAPPTFKA-----EKEMGPTFRAEN--------SVAP 540

Query: 541 TFRAETAPFCPAN-NLNFEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKGN 600
           TFRAETA     N N  F+  G                       +DPFA        GN
Sbjct: 541 TFRAETATLSSQNENRGFDELGF---------------------GNDPFA----GPLNGN 571

Query: 601 DRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           D+SFD     EESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 DQSFD-----EESLLQQQKLWKEQQNKIIAKH 571

BLAST of MS006363.1 vs. ExPASy TrEMBL
Match: A0A1S3BN17 (clathrin coat assembly protein AP180 OS=Cucumis melo OX=3656 GN=LOC103491352 PE=4 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 1.3e-234
Identity = 456/632 (72.15%), Postives = 496/632 (78.48%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGA-NPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSI LAKVSS A  P AT+LEVAVLKATTHDDVPLD+RYV+EIL+
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDVPLDHRYVNEILT 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS+A ACA +IAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S+H     R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHA--TTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI++WQKLLDRAIATRPTGPAK NRLV ++LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIP-SILSDDLEQSESSDR---- 360
           PSDELIETLQEFLKDQASFPCHGNRSPP  +      S+P + L+DDLEQSESSD+    
Sbjct: 301 PSDELIETLQEFLKDQASFPCHGNRSPPQAIL-----SVPMTKLTDDLEQSESSDKNSER 360

Query: 361 ----NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPN 420
               NS+GDIMSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PN
Sbjct: 361 GSDFNSVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NESNLSPN 420

Query: 421 FAFFDDGPQENVKPYQEQLHFG-APSGRLGDWELVLAESATEPPPQEWPDFFAPSIGDDE 480
           FAFF    +EN  P++E+  FG APS R GDWELVLAESAT   P+EWPDFF+PSIGDD 
Sbjct: 421 FAFF----EENGMPFEEESQFGVAPSLRFGDWELVLAESAT-ASPKEWPDFFSPSIGDDL 480

Query: 481 FVKSISPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAP 540
           F K  SPQ  Y NPFLQ +DDLF  PPTF A     E E+ PTFRA+N        +VAP
Sbjct: 481 FAKPFSPQHHYKNPFLQDSDDLFTAPPTFKA-----EKEMGPTFRAEN--------SVAP 540

Query: 541 TFRAETAPFCPAN-NLNFEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKGN 600
           TFRAETA     N N  F+  G                       +DPFA        GN
Sbjct: 541 TFRAETATLSSQNENRGFDELGF---------------------GNDPFA----GPLNGN 571

Query: 601 DRSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
           D+SFD     EESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 DQSFD-----EESLLQQQKLWKEQQNKIIAKH 571

BLAST of MS006363.1 vs. ExPASy TrEMBL
Match: A0A0A0KGJ5 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421580 PE=4 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 3.6e-229
Identity = 450/631 (71.32%), Postives = 490/631 (77.65%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGA-NPAATTLEVAVLKATTHDDVPLDNRYVSEILS 60
           MPSK RKA+ AVKDQTSI LAKVSS A  P AT+LEV VLKATTHDD PLD+RYV+EIL+
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVVVLKATTHDDSPLDHRYVTEILT 60

Query: 61  LIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAK 120
           LI+++KS A ACA +IAKRITKTRNW VALKSLNLVL+IFQDGDPYFPREVLHAMKRGAK
Sbjct: 61  LISANKSNAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHHGPPPNR 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFT RK+ S+H     R
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKAESYHA--TTR 180

Query: 181 RINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRD 240
           RINEPIRDMKPAMLIDRI++WQKLLDRAIATRPTGPAK NRLV ++LHAVV ESFDLYRD
Sbjct: 181 RINEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRD 240

Query: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQ 300
           ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK+IGVGRTSEYPSVQQ
Sbjct: 241 ISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQ 300

Query: 301 PSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESSDR----- 360
           PSDELIETLQEFLKDQASFPCHGNRS PPQ  LP   SI    +DDL+QSESSD+     
Sbjct: 301 PSDELIETLQEFLKDQASFPCHGNRS-PPQPILPG--SITK-FTDDLDQSESSDKNSERG 360

Query: 361 ---NSMGDIMSATGSWASPTNSVEQDGESYSDYQSEKQSQFGDSASMNGNENEQNLTPNF 420
              NS+GDIMSATGSW SP NSVEQDGE YSDYQSEKQS+ GD A      NE NL+PNF
Sbjct: 361 SDFNSVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFA------NELNLSPNF 420

Query: 421 AFFDDGPQENVKPYQEQLHFG-APSGRLGDWELVLAESATEPPPQEWPDFFAPSIGDDEF 480
           AFF    +E+   ++E+  FG APS R GDWELVLAESAT+  P+EWPDFF+PSIGD+ +
Sbjct: 421 AFF----EESGMAFEEESQFGVAPSMRFGDWELVLAESATD-SPKEWPDFFSPSIGDELY 480

Query: 481 VKSISPQQQYNNPFLQGADDLFAVPPTFTAEFSSPETEIAPTFRAQNFKEETVAVAVAPT 540
            K  SPQ  Y NPFLQ +DDLF  PPTF A     E E+ PTFRA+N        +VAPT
Sbjct: 481 AKPFSPQHHYKNPFLQDSDDLFTAPPTFKA-----EKEMGPTFRAEN--------SVAPT 540

Query: 541 FRAETAPFCPAN-NLNFEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKGND 600
           FRAETA     N N  F+  G                       +DPFA        GND
Sbjct: 541 FRAETATLSSQNENCGFDELGF---------------------GNDPFA----GPLNGND 571

Query: 601 RSFDGKTMNEESLLEQQKLWKEQQNKIIAKH 621
             FD     EESLL+QQKLWKEQQNKIIAKH
Sbjct: 601 PIFD-----EESLLQQQKLWKEQQNKIIAKH 571

BLAST of MS006363.1 vs. TAIR 10
Match: AT1G05020.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 492.7 bits (1267), Expect = 4.5e-139
Identity = 324/695 (46.62%), Postives = 411/695 (59.14%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGANPAA--TTLEVAVLKATTHD-DVPLDNRYVSEI 60
           MPSK +KAI AVKDQTSISLAKV++GA      TTLEVA+LKAT+HD +VP+D+R V+EI
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEI 60

Query: 61  LSLIASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRG 120
           L +I+S KS+A +CA+AI +RI +TRNWIVALKSL LVLRIFQDGDPYFPREVLHAMKRG
Sbjct: 61  LGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRG 120

Query: 121 AKILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRK--------- 180
           AKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFLTGKLQ+R+TNR+         
Sbjct: 121 AKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNS 180

Query: 181 -SHSHHGPPPNRRINEP-IRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTL 240
            + S   P    + +EP +RDMKP ML+D+I++WQKLLDRAIATRPTG AK+NRLV  +L
Sbjct: 181 TTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSL 240

Query: 241 HAVVHESFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNI 300
           +AV+ ESFDLYRDISDGLALLLDSFFHLQYQSC+NAFQACV+A+KQFEEL +FYDL K+I
Sbjct: 241 YAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSI 300

Query: 301 GVGRTSEYPSVQQPSDELIETLQEFLKDQASFPCHGNRSPPPQVFLP----AKDSIPSIL 360
           G+GRTSEYPS+Q+ S EL+ETLQEFLKDQ+SFP      P P  FLP    +KDS  S  
Sbjct: 301 GIGRTSEYPSIQKISLELLETLQEFLKDQSSFPASSGLYPSPNSFLPPPPSSKDSAVSSS 360

Query: 361 SD----DLEQSE------SSDRNSMGDIMSAT-GSWASPTNSVEQDGESYSDYQSEKQSQ 420
            D     ++ SE      S    S+ D+MS T    +SP  S     E Y   + +    
Sbjct: 361 LDFGDSTIDTSERYSDYGSFRSTSLEDLMSRTEAGTSSPPMSCH--SEPYGGGRDDPNGN 420

Query: 421 FGDSASMNGNENEQNLTPNFAFFD------------------------------------ 480
             D+ S     N  +++ +    D                                    
Sbjct: 421 NFDTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKAETFDPWEAL 480

Query: 481 ---DGPQENVKPYQEQLHFGAPSGR-LGDWELVLAESATEPPPQEWPDFFAPSIGDDEFV 540
              D P++ ++  +E+        R  G+W L L E+AT+   Q          G D+ +
Sbjct: 481 MLRDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQ--VQGNNSMAIVPFGLDDPM 540

Query: 541 KSISPQQQYNNPFLQG--ADDLFAVPPTFT-AEFSSPETEIAPTFRAQNFKEETVAVAVA 600
            +        NPFL+   A    A  P  T    +    +  PTF+      +    +  
Sbjct: 541 PAFQAATDQYNPFLEEPVAQLATAGEPMITFGGLALTGFQPEPTFQVN--VPDDFEPSST 600

Query: 601 PTFRA-ETAPFCPANNLNFEAWGSTETVVANSTKESNLFSFNPAGEDDPFAVSWRSSEKG 623
           PTF+A ET P        FE++G  ET                             SE G
Sbjct: 601 PTFKATETLPMKCDPFTTFESFGFGETF----------------------------SENG 653

BLAST of MS006363.1 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 2.0e-78
Identity = 160/363 (44.08%), Postives = 228/363 (62.81%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSL 60
           M    RKAI  VKDQTSI +AKV+S   P    LEVA++KAT+HDD    ++Y+ EILSL
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASNMAP---DLEVAIVKATSHDDDQSSDKYIREILSL 60

Query: 61  IASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 120
            + S+ Y  AC +++++R+ KTR+WIVALK+L LV R+  +GDP F  E+L+A +RG +I
Sbjct: 61  TSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRI 120

Query: 121 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTN----RKSHSHHG-- 180
           LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L    ++R  N      SH  +G  
Sbjct: 121 LNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELAL---FERRGRNGGGSSSSHQSNGDD 180

Query: 181 -----------PPPNR-------------------RINE-----------PIRDMKPAML 240
                      PPP                      +NE           P+R+M P  +
Sbjct: 181 GYNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERI 240

Query: 241 IDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRDISDGLALLLDSFFH 300
             ++ H Q+LLDR ++ RPTG AK++R++L  ++ VV ESF LY DI + LA+LLD FF 
Sbjct: 241 FGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFD 300

Query: 301 LQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQPSDELIETLQEFLK 317
           ++Y  CV AF A   AAKQ +EL +FY  CK+ GV R+SEYP VQ+ + +L+ETL+EF++
Sbjct: 301 MEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVR 357

BLAST of MS006363.1 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 2.0e-78
Identity = 160/363 (44.08%), Postives = 228/363 (62.81%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSL 60
           M    RKAI  VKDQTSI +AKV+S   P    LEVA++KAT+HDD    ++Y+ EILSL
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASNMAP---DLEVAIVKATSHDDDQSSDKYIREILSL 60

Query: 61  IASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 120
            + S+ Y  AC +++++R+ KTR+WIVALK+L LV R+  +GDP F  E+L+A +RG +I
Sbjct: 61  TSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRI 120

Query: 121 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTN----RKSHSHHG-- 180
           LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L    ++R  N      SH  +G  
Sbjct: 121 LNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELAL---FERRGRNGGGSSSSHQSNGDD 180

Query: 181 -----------PPPNR-------------------RINE-----------PIRDMKPAML 240
                      PPP                      +NE           P+R+M P  +
Sbjct: 181 GYNRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERI 240

Query: 241 IDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDLYRDISDGLALLLDSFFH 300
             ++ H Q+LLDR ++ RPTG AK++R++L  ++ VV ESF LY DI + LA+LLD FF 
Sbjct: 241 FGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFD 300

Query: 301 LQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPSVQQPSDELIETLQEFLK 317
           ++Y  CV AF A   AAKQ +EL +FY  CK+ GV R+SEYP VQ+ + +L+ETL+EF++
Sbjct: 301 MEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVR 357

BLAST of MS006363.1 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 290.0 bits (741), Expect = 4.4e-78
Identity = 160/398 (40.20%), Postives = 233/398 (58.54%), Query Frame = 0

Query: 1   MPSKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSL 60
           M    RKAI AVKDQTSI +AKV+S   P    LEVA++KAT+HDD P   +Y+ EIL+L
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASNMAP---DLEVAIVKATSHDDDPASEKYIREILNL 60

Query: 61  IASSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKI 120
            + S+ Y  AC +++++R++KTR+W+VALK+L LV R+  +GDP F  E+L++ +RG ++
Sbjct: 61  TSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRM 120

Query: 121 LNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTNRKSHSHH------- 180
           LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L  +      N   +S H       
Sbjct: 121 LNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDR 180

Query: 181 ---------GPPP----------------------------------------------- 240
                     PPP                                               
Sbjct: 181 YGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGR 240

Query: 241 -NRRINEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHESFDL 300
             +++  P+R+M P  +  ++ H Q+LLDR ++ RPTG AK++R++L  L+ VV ESF L
Sbjct: 241 DEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKL 300

Query: 301 YRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTSEYPS 331
           Y DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEYP 
Sbjct: 301 YADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPE 360

BLAST of MS006363.1 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 277.3 bits (708), Expect = 3.0e-74
Identity = 163/466 (34.98%), Postives = 266/466 (57.08%), Query Frame = 0

Query: 3   SKFRKAIDAVKDQTSISLAKVSSGANPAATTLEVAVLKATTHDDVPLDNRYVSEILSLIA 62
           SKF++AI AVKDQTS+ LAKV +G + + + L+VA++KAT H++ P + +Y+ EILSL +
Sbjct: 4   SKFKRAIGAVKDQTSVGLAKV-NGRSASLSELDVAIVKATRHEEFPAEEKYIREILSLTS 63

Query: 63  SSKSYAPACASAIAKRITKTRNWIVALKSLNLVLRIFQDGDPYFPREVLHAMKRGAKILN 122
            S+SY  AC S +++R+ KT+ W VALK+L L+ R+  +GD  + +E+  A +RG ++LN
Sbjct: 64  YSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRLLN 123

Query: 123 LSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKR-------FTNRKSHSHHGP 182
           +S+FRD S S+ WDY+AFVRT+ALYLDERLD  +  +  KR         + +       
Sbjct: 124 MSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQAAA 183

Query: 183 PPNRRI---NEPIRDMKPAMLIDRISHWQKLLDRAIATRPTGPAKSNRLVLYTLHAVVHE 242
             +  I   ++PI +MK   +  RI H Q+LLDR +A RPTG A++NR+V+  L+ +V E
Sbjct: 184 DLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKE 243

Query: 243 SFDLYRDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKNIGVGRTS 302
           SF +Y D+++ + +L++ F  L     +  +    + +KQFEEL  FY  CKN+G+ R+S
Sbjct: 244 SFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSS 303

Query: 303 EYPSVQQPSDELIETLQEFLKDQASFPCHGNRSPPPQVFLPAKDSIPSILSDDLEQSESS 362
           EYP +++ + + ++ + EF++D+++   H  +S           S+ S   +D +++ + 
Sbjct: 304 EYPEIEKITQKKLDLMDEFIRDKSALE-HTKQS----------KSVKSEADEDDDEARTE 363

Query: 363 DRNSMGDIMSATGSWASPTNSVEQDGESYSDYQSE-----KQSQFGDSASM---NGNENE 422
           + N   + M+A  +   P    E D +   + + E     KQ + GD   +   NG E  
Sbjct: 364 EVNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAG 423

Query: 423 QNLTPNFAFFDDGPQENVKPYQEQLHFGAPSGRLGDWELVLAESAT 451
           Q          DGP  +    +    + A      DWE  L ++AT
Sbjct: 424 QAGDSLALALFDGPYASGSGSESGPGWEAFKDDSADWETALVQTAT 457