MS005701.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS005701.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionreplication factor C subunit 3-like
Locationscaffold254: 927660 .. 932180 (+)
Sequence length1827
RNA-Seq ExpressionMS005701.1
SyntenyMS005701.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACGACGACAATAACAACAACCCATCTCTCCGCCGCTCCGTGTCGGACCCCCGCCTCTCCCGCCACCGCCGGCGCCGCCGCACGTTCTCCACCACCTCCTCCAAGTCCTCATGGAGCTCGAAGCTGGCCAAAATCCTGGCCCGGCTGGCCCTCTTCTCCAGCGACTCTGAGCTGACAGAAGAAAGCCTCGAAGCCCACAACAAGAGAATCAGAGAAGAACTCGAACAACGACAGCTACACGACAAAACCCCCAAATCCAGCCCCTACTACCGCGGCCTCACCGACTCCTCCCTCGCCATCAACTACCACCATGGGCCCCTCGCCACTCCCGGCCCGGCCCATCAACAACTCAGTGGGCCCCACTACGCCAGCTCATCCGCCACCCAAGTCACGTCCTTCTCCTCCATCGTCGTCTCCAAGCTCAAGGGGTGGGCCCCATGCCTCGGCAAATCCAACGACAAACCGGCCCCCGGCCCCGACAAGAACAACAATAACAATAGCATCATCCAAAACATCCCCACCGTGACCAAAACGGCGTCGTCTTCGTCGTCAAAGCCGAAGCCGAGGCCAAAGCCAAGGCCGAAGCCGGAGCCGGATCCGGAGCCGGAGCCGGAGCCGGTCAGGAGATCGGAGGAGGCGTTGAATATTAGTAATGATGATGGTGATCATCAAGAGAAGCTGTTGAGGGAGAGATCATTACAGAGCCGGCGAGGCGGCGGCGGGGGAGAGGTGGCGGAGAAGGAGAGGTACACGTGGGGGGACAAGTACAGGCCGAAGGTGCTGGAGGAGTTCATATGCAACAGGAGGAAGGCGAGGCAGCTGAAGGAGGTGGTGAGAGAGAAGGGGTGCGGGAATTACATATTTGAAGGACCGCCGGGAGTGGGAAAGAGGACCATGATTCGGGCCATGCTTCGAGAAGCTTTTGGACACCAAGCAATGGAGGTGTATTGTATTATCAGTATGACTTATCACACTTCAAACCCTTCCCTATCTATTAATTTTCCCATATACATATAAAATCCACACGCACCCTACCGCGTTGGGTAGTGGTAAGAGGTCTCGGTGGTTGGCACCCAGGATTACTACAATATAACTATTAGAACTTTAGAATATTCGTAACCAAGGAAAATACTTAATTTTTTAGACTAATCTTGTTTTAACTATAACTCGAGGATAACTAACGATGAACTGTCAATTTTTCAATTGTAATTAATATTTAATAGAGGGATAGGTTGTTTTCACCACTTAAGAAGTAAAATCTTTAGTTCTTTATTACTCGAGACCTATCTTGTCCTAACCACTATTTGACAACTAATGATGAGTTGTCAATTTTCTAATGGTGGTTAGGATAGAAATAGGTCGTCCTCAATAGTTAGGAAGGGAAATACTTAGGTTCGGGTAAAAACCTATTCTTATGTTGACCACCATTTGAAAATTTGACCCCTCATCATATTTCTAATAAAATTATTTCTTTTTATAATTCTGTCTTCTAGTTATCAAATATTTAATGATAGTGAGCACAAGATTATTAATCTTCATAGGTTCCATTTCAAAATCATACAATGAGAGATCGAGGAATAACTCAGTTATATTATAAACTCGTATTAGATTTCTTATCTTTTTAATGTGTCCTTTTATCTTTTTTATCTTTTTAGTGTGGGATTCTTAATAAAGATAACTTCTCATGGTCTAAATATTTTCCTATTTAATTATAATAATTTTTGCCCGAAAATACTTCGTTTTGGAATGGGAATTTAGCTAACTTTTCTTTGTTCCCTCTTCGTTTATTTCTCACAGATTAAGGAAGTTGTCAGGGTTTTTGACTTGAAGGTAAAGAAACTACATTTCTCTTGTGTGTGTTTTTTTTTCTTTCTTCCTTCTACACACACAGAGAAAAAATAATTTCTTATTTTTTTGCTTCATTTTGCATTATTATTAATATTTTTTGTGACACGTTTGCAGAGTGAAATGGTGGGGAGAATAGAAGTAAAGGTGAGGGAATCATCTCACTACGTGGAAGTCAATCTTTCACAAACCAAAGGTTTTGAGAAACAAGTCATCGTCCAACTCATCAAAGAAACTCACTCCCCTTTGCCATGTAATCATTCCAATTGTCAAGGTATCACTTGAGAATCCATATAAATGAATTAAATTTACCTAATCTATCAGTTTAAGTTTTTAGGTTGAGTGATGATTTATTCATCCGTATCAGAACGAGAGCTCTTGTATTCGAACCCTCGTACGTGCGTTTGCTTAAAAGAACAATTTTTTCAAAAGGTAACCCAAATTAAACTCTTTTTAATCCGGTTTTGTTTTACTAAACATGATAGTCCAACCAACCTAGCTCTATTGATTAAGGTATCAATAAAACTTACCTTTCGTGTGTTTGAATTCCACACTCATTAATTTGACAAACGTAATTTTGATGAGTGATAGACTTTGGGTCGCATATTTAAATTCTCTATCTTAGTCCTCTGGGAATGATGCAGTAGCAAGAGCATAAGGCTTTATACGGTATCATCTACCATGACGTTTTGTGTTCAAGCACTGTACTGGTCACAATATGTTGCTCAATCCAAACAACGAGTCTCGATTATATTGGGTCTAAGCTACACAAAAAAAAAAAAAAAACTCTATCTTAATATATTAGAAGTATGGGACAAGTAAATTAGAAATGATGTATATTTTTGTAAATGGTTGTTGTAGGGATATTGCTGTGTGAAGCAGAAAAGCTATCAAATGAAACCCTAATGTATGTAAAATGGGCGATTGAAAGGAACAAAGGATGCAGCAAAATCTTCTTCTGCTGCTCTGATGTCTCCAAGCTTCTCCCTCTTACCTCCCTCTGCACTCTCATTCACCTCTCCCCACCTTCAAAACAAGAGGTAAATTCATTTTTTATTTTTTATTTTGTGTTCCAAAAATTTAAAGCTTATAATTATAAAACCTACTTGTGTTGTGTTTTTTCCTTTTTATTTTTTTAAAAATGTTAGTCGAATTTCTAAAACAAAAGTTCTCGAAAACTATTTTTAGCTTTCAAATTTTGACTTTCATTTCAAGAACACATTTTTAAAGAGTAGATAAGAAAAGAAAAAAAAAATGTTAGTATGATTAATAATAATTTTTAAAAAACAGAAAACCAAATTAAAGAAATTTCCAAATTTGTAAAAACTTATAACTATGTTCCAATCTATTTTTATATTTGTCCTATTTTTTATGCCGACTTCATAGATGTGTCGATGCAAATTTAACATTGATCAACACATTAAAATGAAATAAGAAAAAAAAACTATTATAAAAACATAAATAAATTATTTGATTTTTTTTTTATGCACAATATTGTGTGTACCGTACGTAGATTGTGGAAGTCCTCGAATTCGTAGCAAAACAAGAAGGATTCGACTTGTCGACTCGGTTGGCGGAGAGGATCGCCGACAATTCCAAGAACAACCTCCGACAAGCCATTCGATCCCTAGAAGCTTCCTGGAATAAAAGGTCAGTTTTGTCAATTCATTCATTAATTAATAAATTATTATTATTATTATTTAGTTTTTGGTTGAGTGAATTTAGAATGGTGAAATGAATTTGTAGTCAATTGTTTGAAGAAGATGAGAATCAACTGTTGACTGGTTGGGAAGATGATATTGCAGATGTTGCTAAGAAGATTGTCGAGGAACAAAGCCCAAAACAGTAATTAACTAATTAATTTTCCCGGAAAGTTTTTGTTTCTTTTTTTCCGGGAAAATAAAGAACTTCTCTCTAAAATCTATTGTTTTTGGTATTTTTATGCAGGTTGTATATTGTTCGTGGAAAGCTTAAAAAACTTATCGAACATGATGTCTCCCCCAACTTCATTTTTAGGGTAATTTTCTCTCTCAATTTCGCCGCCACAATGTGTTTGTCTTTAATAATCGTGTAATACAGTTATTAAAATTTAAATTTAGTAGGTAAATTTTGTCTATCTTTATAAACTTTCTAGTTTCATTAGTCGCTTTTTTCTTTTAATACAACGAGTAGAGGTGAAGATTCAAAGTCACAATTTTTTTTACTGGAGATAGATTTTTAATCTAGTTGAGCACTGTTCAGGTTTGCGATTTTATTGGTGGCTTTCTCTTCTTTTTATAATGTAATAATTATGCATGTGTAGACTTTGGTGGACGAATTAAAGAAGTTTTTGGACGAAGAATTACAGCGTCGAATTGAAGGTCTTTATGCTGATTATAACGTAAGTTTGTAAGTTCATTTGATTTTAAATTTGCACTAAGAATTTTTTTTTTCTAGGGAATTAAAAAGTTAGCATATAATATGAATTATTATGAACAAGAAAAATCAATTGTGAATGAAAATATTTAAAGAATATAATTGTGGATGTGAATTATTGAATTTCAGAAAATAGAGGAAAAGACATTTGTTAGTGATCAACAAGGCAATGGGGAAGAGGCAGTGGCCAAAGTGCTGCATGACCCTATGAGAAAGAACGTTCACCACTTCATGAAGATCGAAGGTAAAATAAAA

mRNA sequence

ATGGCCAACGACGACAATAACAACAACCCATCTCTCCGCCGCTCCGTGTCGGACCCCCGCCTCTCCCGCCACCGCCGGCGCCGCCGCACGTTCTCCACCACCTCCTCCAAGTCCTCATGGAGCTCGAAGCTGGCCAAAATCCTGGCCCGGCTGGCCCTCTTCTCCAGCGACTCTGAGCTGACAGAAGAAAGCCTCGAAGCCCACAACAAGAGAATCAGAGAAGAACTCGAACAACGACAGCTACACGACAAAACCCCCAAATCCAGCCCCTACTACCGCGGCCTCACCGACTCCTCCCTCGCCATCAACTACCACCATGGGCCCCTCGCCACTCCCGGCCCGGCCCATCAACAACTCAGTGGGCCCCACTACGCCAGCTCATCCGCCACCCAAGTCACGTCCTTCTCCTCCATCGTCGTCTCCAAGCTCAAGGGGTGGGCCCCATGCCTCGGCAAATCCAACGACAAACCGGCCCCCGGCCCCGACAAGAACAACAATAACAATAGCATCATCCAAAACATCCCCACCGTGACCAAAACGGCGTCGTCTTCGTCGTCAAAGCCGAAGCCGAGGCCAAAGCCAAGGCCGAAGCCGGAGCCGGATCCGGAGCCGGAGCCGGAGCCGGTCAGGAGATCGGAGGAGGCGTTGAATATTAGTAATGATGATGGTGATCATCAAGAGAAGCTGTTGAGGGAGAGATCATTACAGAGCCGGCGAGGCGGCGGCGGGGGAGAGGTGGCGGAGAAGGAGAGGTACACGTGGGGGGACAAGTACAGGCCGAAGGTGCTGGAGGAGTTCATATGCAACAGGAGGAAGGCGAGGCAGCTGAAGGAGGTGGTGAGAGAGAAGGGGTGCGGGAATTACATATTTGAAGGACCGCCGGGAGTGGGAAAGAGGACCATGATTCGGGCCATGCTTCGAGAAGCTTTTGGACACCAAGCAATGGAGATTAAGGAAGTTGTCAGGGTTTTTGACTTGAAGAGTGAAATGGTGGGGAGAATAGAAGTAAAGGTGAGGGAATCATCTCACTACGTGGAAGTCAATCTTTCACAAACCAAAGGTTTTGAGAAACAAGTCATCGTCCAACTCATCAAAGAAACTCACTCCCCTTTGCCATGTAATCATTCCAATTGTCAAGGGATATTGCTGTGTGAAGCAGAAAAGCTATCAAATGAAACCCTAATGTATGTAAAATGGGCGATTGAAAGGAACAAAGGATGCAGCAAAATCTTCTTCTGCTGCTCTGATGTCTCCAAGCTTCTCCCTCTTACCTCCCTCTGCACTCTCATTCACCTCTCCCCACCTTCAAAACAAGAGATTGTGGAAGTCCTCGAATTCGTAGCAAAACAAGAAGGATTCGACTTGTCGACTCGGTTGGCGGAGAGGATCGCCGACAATTCCAAGAACAACCTCCGACAAGCCATTCGATCCCTAGAAGCTTCCTGGAATAAAAGTCAATTGTTTGAAGAAGATGAGAATCAACTGTTGACTGGTTGGGAAGATGATATTGCAGATGTTGCTAAGAAGATTGTCGAGGAACAAAGCCCAAAACAGTTGTATATTGTTCGTGGAAAGCTTAAAAAACTTATCGAACATGATGTCTCCCCCAACTTCATTTTTAGGACTTTGGTGGACGAATTAAAGAAGTTTTTGGACGAAGAATTACAGCGTCGAATTGAAGGTCTTTATGCTGATTATAACAAAATAGAGGAAAAGACATTTGTTAGTGATCAACAAGGCAATGGGGAAGAGGCAGTGGCCAAAGTGCTGCATGACCCTATGAGAAAGAACGTTCACCACTTCATGAAGATCGAAGGTAAAATAAAA

Coding sequence (CDS)

ATGGCCAACGACGACAATAACAACAACCCATCTCTCCGCCGCTCCGTGTCGGACCCCCGCCTCTCCCGCCACCGCCGGCGCCGCCGCACGTTCTCCACCACCTCCTCCAAGTCCTCATGGAGCTCGAAGCTGGCCAAAATCCTGGCCCGGCTGGCCCTCTTCTCCAGCGACTCTGAGCTGACAGAAGAAAGCCTCGAAGCCCACAACAAGAGAATCAGAGAAGAACTCGAACAACGACAGCTACACGACAAAACCCCCAAATCCAGCCCCTACTACCGCGGCCTCACCGACTCCTCCCTCGCCATCAACTACCACCATGGGCCCCTCGCCACTCCCGGCCCGGCCCATCAACAACTCAGTGGGCCCCACTACGCCAGCTCATCCGCCACCCAAGTCACGTCCTTCTCCTCCATCGTCGTCTCCAAGCTCAAGGGGTGGGCCCCATGCCTCGGCAAATCCAACGACAAACCGGCCCCCGGCCCCGACAAGAACAACAATAACAATAGCATCATCCAAAACATCCCCACCGTGACCAAAACGGCGTCGTCTTCGTCGTCAAAGCCGAAGCCGAGGCCAAAGCCAAGGCCGAAGCCGGAGCCGGATCCGGAGCCGGAGCCGGAGCCGGTCAGGAGATCGGAGGAGGCGTTGAATATTAGTAATGATGATGGTGATCATCAAGAGAAGCTGTTGAGGGAGAGATCATTACAGAGCCGGCGAGGCGGCGGCGGGGGAGAGGTGGCGGAGAAGGAGAGGTACACGTGGGGGGACAAGTACAGGCCGAAGGTGCTGGAGGAGTTCATATGCAACAGGAGGAAGGCGAGGCAGCTGAAGGAGGTGGTGAGAGAGAAGGGGTGCGGGAATTACATATTTGAAGGACCGCCGGGAGTGGGAAAGAGGACCATGATTCGGGCCATGCTTCGAGAAGCTTTTGGACACCAAGCAATGGAGATTAAGGAAGTTGTCAGGGTTTTTGACTTGAAGAGTGAAATGGTGGGGAGAATAGAAGTAAAGGTGAGGGAATCATCTCACTACGTGGAAGTCAATCTTTCACAAACCAAAGGTTTTGAGAAACAAGTCATCGTCCAACTCATCAAAGAAACTCACTCCCCTTTGCCATGTAATCATTCCAATTGTCAAGGGATATTGCTGTGTGAAGCAGAAAAGCTATCAAATGAAACCCTAATGTATGTAAAATGGGCGATTGAAAGGAACAAAGGATGCAGCAAAATCTTCTTCTGCTGCTCTGATGTCTCCAAGCTTCTCCCTCTTACCTCCCTCTGCACTCTCATTCACCTCTCCCCACCTTCAAAACAAGAGATTGTGGAAGTCCTCGAATTCGTAGCAAAACAAGAAGGATTCGACTTGTCGACTCGGTTGGCGGAGAGGATCGCCGACAATTCCAAGAACAACCTCCGACAAGCCATTCGATCCCTAGAAGCTTCCTGGAATAAAAGTCAATTGTTTGAAGAAGATGAGAATCAACTGTTGACTGGTTGGGAAGATGATATTGCAGATGTTGCTAAGAAGATTGTCGAGGAACAAAGCCCAAAACAGTTGTATATTGTTCGTGGAAAGCTTAAAAAACTTATCGAACATGATGTCTCCCCCAACTTCATTTTTAGGACTTTGGTGGACGAATTAAAGAAGTTTTTGGACGAAGAATTACAGCGTCGAATTGAAGGTCTTTATGCTGATTATAACAAAATAGAGGAAAAGACATTTGTTAGTGATCAACAAGGCAATGGGGAAGAGGCAGTGGCCAAAGTGCTGCATGACCCTATGAGAAAGAACGTTCACCACTTCATGAAGATCGAAGGTAAAATAAAA

Protein sequence

MANDDNNNNPSLRRSVSDPRLSRHRRRRRTFSTTSSKSSWSSKLAKILARLALFSSDSELTEESLEAHNKRIREELEQRQLHDKTPKSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSGPHYASSSATQVTSFSSIVVSKLKGWAPCLGKSNDKPAPGPDKNNNNNSIIQNIPTVTKTASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRERSLQSRRGGGGGEVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYIFEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAKVLHDPMRKNVHHFMKIEGKIK
Homology
BLAST of MS005701.1 vs. NCBI nr
Match: KAG6597225.1 (Replication factor C subunit 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 749.6 bits (1934), Expect = 2.1e-212
Identity = 423/616 (68.67%), Postives = 479/616 (77.76%), Query Frame = 0

Query: 5   DNNNNPSLRRSVSDPRLSRHRRRRRTFST--TSSKSSWSSKLAKILARLALFSSDSELTE 64
           +++++ SLRRS+SD    R +RRR + ST  +SSKSSWS+KLAK+LARL LFS +S+LTE
Sbjct: 9   NSSSSSSLRRSLSDSNTRRRQRRRGSISTASSSSKSSWSTKLAKLLARLNLFSRNSDLTE 68

Query: 65  ESLEAHNKRIREELEQRQLHDKTP-KSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSG 124
           ESL+AHN+RI +        DKTP KSSPYYRGLTDSSLAINYHHGPL T         G
Sbjct: 69  ESLQAHNERIDD-------LDKTPLKSSPYYRGLTDSSLAINYHHGPLTT---------G 128

Query: 125 PHYASSSATQV---TSFSSIVVSKLKGWAPCL-GKSNDKPAPGPDKNNNNNSIIQNIPTV 184
           PH+ +SS T     TS S  VVSKLK WAPCL GK N KP           + +  +   
Sbjct: 129 PHHYASSTTTAQTQTSLSYAVVSKLKEWAPCLIGKPNTKPPAHQQYETPTTTTV--VGKT 188

Query: 185 TKTASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRERSLQ- 244
           T  A SSS                    PE + R            + +EKLLRER +  
Sbjct: 189 TSLAVSSS--------------------PEKIERWAT---------EKEEKLLRERLVMY 248

Query: 245 -SRRGG------GGGEVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYI 304
             R GG      GGGE AEKERYTWGDKYRPKVLE+FICNR+ A +LK++V EKGCG+YI
Sbjct: 249 GRREGGSGVGVDGGGEAAEKERYTWGDKYRPKVLEDFICNRKTACELKKLVEEKGCGHYI 308

Query: 305 FEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNL 364
           FEGPPGVGKRTMI+AMLR+AFG QAME+KEV RVF LKSEMVG IEVKV+ESSH VEVNL
Sbjct: 309 FEGPPGVGKRTMIQAMLRQAFGEQAMEVKEVDRVFALKSEMVGSIEVKVKESSHCVEVNL 368

Query: 365 SQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSK 424
           SQTKGFEKQVI QLIKET SPLPCNH+ C+GILLCEA++LSNETLMYVKWAIERNKGCSK
Sbjct: 369 SQTKGFEKQVIDQLIKETQSPLPCNHARCRGILLCEADQLSNETLMYVKWAIERNKGCSK 428

Query: 425 IFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKN 484
           IFFCCSDVSKLL L+S CT +HLSPPSKQEIVEVLE++AKQ+ FDLS R+AERIADNSKN
Sbjct: 429 IFFCCSDVSKLLLLSSFCTHVHLSPPSKQEIVEVLEYIAKQQEFDLSRRMAERIADNSKN 488

Query: 485 NLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL 544
           NLRQAIRSLEASW KS+LFEEDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL
Sbjct: 489 NLRQAIRSLEASWKKSRLFEEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL 548

Query: 545 IEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAKV 604
           IE+DVSPNFIF+TLVDELKKFLDEELQRR+E  YADYNK+EEK FV+ ++GNGEEAV K 
Sbjct: 549 IEYDVSPNFIFKTLVDELKKFLDEELQRRVESFYADYNKMEEKPFVT-EKGNGEEAVIKE 576

Query: 605 LHDPMRKNVHHFMKIE 606
           LHDPMRKNV+HF+KIE
Sbjct: 609 LHDPMRKNVNHFLKIE 576

BLAST of MS005701.1 vs. NCBI nr
Match: XP_022946202.1 (replication factor C subunit 3-like [Cucurbita moschata])

HSP 1 Score: 748.8 bits (1932), Expect = 3.6e-212
Identity = 423/616 (68.67%), Postives = 478/616 (77.60%), Query Frame = 0

Query: 5   DNNNNPSLRRSVSDPRLSRHRRRRRTFST--TSSKSSWSSKLAKILARLALFSSDSELTE 64
           +++++ SLRRS+SD    R +RRR + ST  +SSKSSWS+KLAK+LARL LFS +S+LTE
Sbjct: 9   NSSSSSSLRRSLSDSNTRRRQRRRGSISTASSSSKSSWSTKLAKLLARLNLFSRNSDLTE 68

Query: 65  ESLEAHNKRIREELEQRQLHDKTP-KSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSG 124
           ESL+AHN+RI +        DKTP KSSPYYRGLTDSSLAINYHHGPL T         G
Sbjct: 69  ESLQAHNERIDD-------LDKTPLKSSPYYRGLTDSSLAINYHHGPLTT---------G 128

Query: 125 PHYASSSATQV---TSFSSIVVSKLKGWAPCL-GKSNDKPAPGPDKNNNNNSIIQNIPTV 184
           PH+ +SS T     TS S  VVSKLK WAPCL GK N KP           + +  +   
Sbjct: 129 PHHYASSTTTAQTQTSLSYAVVSKLKEWAPCLIGKPNTKPPAHQQYETPTTTTV--VGKT 188

Query: 185 TKTASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRERSLQ- 244
           T  A SSS                    PE + R            + +EKLLRER +  
Sbjct: 189 TSLAVSSS--------------------PEKIERWAT---------EKEEKLLRERLVMY 248

Query: 245 -SRRGG------GGGEVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYI 304
             R GG      GGGE AEKERYTWGDKYRPKVLE+FICNR+ A +LK+VV EKGCG+YI
Sbjct: 249 GRREGGSGVGVDGGGEAAEKERYTWGDKYRPKVLEDFICNRKTACELKKVVEEKGCGHYI 308

Query: 305 FEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNL 364
           FEGPPGVGKRTMI+AMLR+AFG QAME+KEV RVF LKSEMVG IEVKV+ESSH VEVNL
Sbjct: 309 FEGPPGVGKRTMIQAMLRQAFGEQAMEVKEVDRVFALKSEMVGSIEVKVKESSHCVEVNL 368

Query: 365 SQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSK 424
           SQTKGFEKQVI QLIKET SPLPCNH+ C+GILLCEA++LSNETLMYVKWAIERNKGCSK
Sbjct: 369 SQTKGFEKQVIDQLIKETQSPLPCNHARCRGILLCEADQLSNETLMYVKWAIERNKGCSK 428

Query: 425 IFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKN 484
           IFFCCSDVSKLL L+S CT +HLSPPSKQEIVEVLE++AKQ+ FDLS R+AERIADNSKN
Sbjct: 429 IFFCCSDVSKLLLLSSFCTHVHLSPPSKQEIVEVLEYIAKQQEFDLSRRMAERIADNSKN 488

Query: 485 NLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL 544
           NLRQAIRSLEASW KS+LFEEDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL
Sbjct: 489 NLRQAIRSLEASWKKSRLFEEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL 548

Query: 545 IEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAKV 604
           IE+DVSPNFIF+TLVDELKKFLDEELQ R+E  YADYNK+EEK FV+ ++GNGEEAV K 
Sbjct: 549 IEYDVSPNFIFKTLVDELKKFLDEELQHRVESFYADYNKMEEKPFVT-EKGNGEEAVIKE 576

Query: 605 LHDPMRKNVHHFMKIE 606
           LHDPMRKNV+HF+KIE
Sbjct: 609 LHDPMRKNVNHFLKIE 576

BLAST of MS005701.1 vs. NCBI nr
Match: XP_022975501.1 (replication factor C subunit 3-like [Cucurbita maxima])

HSP 1 Score: 746.1 bits (1925), Expect = 2.4e-211
Identity = 425/617 (68.88%), Postives = 480/617 (77.80%), Query Frame = 0

Query: 7   NNNPSLRRSVSDPRLSRHRRRRRTFST--TSSKSSWSSKLAKILARLALFSSDSELTEES 66
           +++ SLRRS+SD    R +RR  + ST  +SSKSSWS+KLAK+LARL LFS +S+LTEES
Sbjct: 11  SSSSSLRRSLSDSNTRRRQRRHWSISTASSSSKSSWSTKLAKLLARLNLFSRNSDLTEES 70

Query: 67  LEAHNKRIREELEQRQLHDKTP-KSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSGPH 126
           L+AHN+RI +        DKTP KSSPYYRGLTDSSLAINYHHGPL T    H      H
Sbjct: 71  LQAHNERIDD-------LDKTPLKSSPYYRGLTDSSLAINYHHGPLTT---THVTTGPHH 130

Query: 127 YASSSAT--QVTSFSSIVVSKLKGWAPCL-GKSNDKPAPGPDKNNNNNSIIQNIPTVTKT 186
           YASS+ T    TS S  VVSKLK WAPCL GK N KP   P  + +       +   T +
Sbjct: 131 YASSTTTAQTQTSLSYAVVSKLKEWAPCLIGKPNKKP---PAHHQHETPTTTTVVGKTTS 190

Query: 187 ASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRER-SLQSRR 246
            ++SSS                   PE + R            + +EKLLRER  +  RR
Sbjct: 191 LATSSS-------------------PEKIERLAT---------EKEEKLLRERLVMYGRR 250

Query: 247 GG-------GGGEVA----EKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNY 306
            G       GGGE +    EKERYTWGDKYRPKVLE+FICNR+ A +LK+VV EKGCG+Y
Sbjct: 251 EGRSGVGVDGGGEASEKEKEKERYTWGDKYRPKVLEDFICNRQTACELKKVVEEKGCGHY 310

Query: 307 IFEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVN 366
           IFEGPPGVGKRTMI+AMLR+AFG QAME+KEV RVF LKSEMVG IEVKV+ESSH VEVN
Sbjct: 311 IFEGPPGVGKRTMIQAMLRQAFGEQAMEVKEVGRVFALKSEMVGSIEVKVKESSHCVEVN 370

Query: 367 LSQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCS 426
           LSQTKGFEKQVI QLIKET SPLPCNH+ C+GILLCEA++LSNETLMYVKWAIERNKGCS
Sbjct: 371 LSQTKGFEKQVIDQLIKETQSPLPCNHARCRGILLCEADQLSNETLMYVKWAIERNKGCS 430

Query: 427 KIFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSK 486
           KIFFCCSDVSKLL L+SLCT +HLSPPSKQEIVEVLE++AKQ+ FDLS R+AERIADNSK
Sbjct: 431 KIFFCCSDVSKLLLLSSLCTHVHLSPPSKQEIVEVLEYIAKQQEFDLSRRMAERIADNSK 490

Query: 487 NNLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK 546
           NNLRQAIRSLEASW KS+LFEEDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK
Sbjct: 491 NNLRQAIRSLEASWKKSRLFEEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK 550

Query: 547 LIEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAK 606
           LIE+DVSPNFIF+TLVDELKKFLDEELQRR+E  YADYNK+EEK FV+D +GNGEEAV K
Sbjct: 551 LIEYDVSPNFIFKTLVDELKKFLDEELQRRVESFYADYNKMEEKPFVTD-KGNGEEAVIK 585

BLAST of MS005701.1 vs. NCBI nr
Match: XP_008438224.1 (PREDICTED: replication factor C subunit 3-like [Cucumis melo])

HSP 1 Score: 722.6 bits (1864), Expect = 2.8e-204
Identity = 412/619 (66.56%), Postives = 476/619 (76.90%), Query Frame = 0

Query: 1   MANDDNNNNPSLRRSVSDPRLSRHR-RRRRTFSTTSSKSSWSSKLAKILARLALFSSDSE 60
           MA       PSLRRS+SD    R R RRR T STTSSKSSWSSKL K+LARLAL S DS+
Sbjct: 1   MATQQQLQQPSLRRSLSDSNHRRRRSRRRATISTTSSKSSWSSKLGKLLARLALLSRDSD 60

Query: 61  LTEESLEAHNKRIREELEQRQLHDKTPKSSPYYRGLTDSSLAINYHHGPLATPGPAHQQL 120
           LTEESLEAHNKRI +        +KTPKSSPYYRGLTDSSLAINYHHGPL          
Sbjct: 61  LTEESLEAHNKRIND----LDTLEKTPKSSPYYRGLTDSSLAINYHHGPLN--------- 120

Query: 121 SGPHYASSSATQVTSFSSIVVSKLKG-WAPCLGKSNDKPAP--------GPDKNNNNNSI 180
           S P+  + + +   S  S +VSK K  WAPCL K   +  P         P ++    ++
Sbjct: 121 STPYNVTYATSAAPSTQSSIVSKFKEYWAPCLRKQQQQQQPPHTTPHKVPPRQHQRIQTV 180

Query: 181 IQNIPTVTKTASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLL 240
             +   VTK    SS         + +   D     + + R  E L +SN  G+      
Sbjct: 181 TTSTTVVTKATWFSSGSTSASANFKEEMSSDEMMTKKKLLR--ERLVVSN-GGER----- 240

Query: 241 RERSLQSRRGGGG---GEVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGN 300
           R+R   S  GGGG    E  EKERY+WGD YRPK LE+FICN++ A +LKE+V+EKGCG+
Sbjct: 241 RDRGGGSGSGGGGVVVVEEMEKERYSWGDNYRPKFLEDFICNKKTAIELKEMVKEKGCGH 300

Query: 301 -YIFEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVE 360
            YIFEG PGVGKRTMI+AMLREAFG+Q++EIKEVV+VFDLKSEM+G IEVKV+ESSHYVE
Sbjct: 301 YYIFEGAPGVGKRTMIQAMLREAFGNQSVEIKEVVKVFDLKSEMLGSIEVKVKESSHYVE 360

Query: 361 VNLSQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKG 420
           VN+SQTKGFEKQVIVQL+KE+HSPLPCNH+NC+GILLCEA++LSNETLMYVKWAIERNKG
Sbjct: 361 VNMSQTKGFEKQVIVQLMKESHSPLPCNHANCRGILLCEADQLSNETLMYVKWAIERNKG 420

Query: 421 CSKIFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADN 480
           C+KIFFCC+D SKL  L+S+CTL+HLSPPSKQEIVEVLEF+AKQ+GFDLS RLAERIADN
Sbjct: 421 CTKIFFCCTDASKLHLLSSVCTLVHLSPPSKQEIVEVLEFIAKQQGFDLSKRLAERIADN 480

Query: 481 SKNNLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKL 540
           SKNNLRQAIRSLEASW KSQLF+EDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKL
Sbjct: 481 SKNNLRQAIRSLEASWKKSQLFDEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKL 540

Query: 541 KKLIEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAV 600
           KKLIE+DVSPNFIFRTLVDELKKFLDEELQRR+EG YADY K+EEK FVS ++G+GEE V
Sbjct: 541 KKLIEYDVSPNFIFRTLVDELKKFLDEELQRRVEGFYADYYKLEEKAFVS-ERGSGEETV 597

Query: 601 AKVLHDPMRKNVHHFMKIE 606
            K  +DP+RKNV+HF+KIE
Sbjct: 601 VKGQNDPVRKNVNHFLKIE 597

BLAST of MS005701.1 vs. NCBI nr
Match: XP_011650816.1 (replication factor C subunit 3 [Cucumis sativus])

HSP 1 Score: 722.6 bits (1864), Expect = 2.8e-204
Identity = 411/617 (66.61%), Postives = 472/617 (76.50%), Query Frame = 0

Query: 10  PSLRRSVSDPRLSRHRRRRR-TFSTTSSKSSWSSKLAKILARLALFSSDSELTEESLEAH 69
           PSLRRS+SD    R R RRR T STTSSKSSWSSKL K+LARLA  S DS+LTEESLEAH
Sbjct: 9   PSLRRSLSDSNHRRRRNRRRATISTTSSKSSWSSKLGKLLARLAFLSRDSDLTEESLEAH 68

Query: 70  NKRIREELEQRQLHDKTPKSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSGPHYASSS 129
           NKRI +        DKTPKSSPYYRGLTDSSLAINYHHGPL          S P++ + +
Sbjct: 69  NKRIND----LDTLDKTPKSSPYYRGLTDSSLAINYHHGPLN---------STPYHVTYA 128

Query: 130 ATQVTSFSSIVVSKLKGW-APCLGKSN-DKPAPGPDKNNNNNSIIQNIPTVTKTASSSS- 189
            +   S  S +VSK K + APCL K     P   P K    +  IQ + T T   + ++ 
Sbjct: 129 TSAAPSTQSSIVSKFKDYMAPCLRKQQPQSPQTSPRKVPRQHQRIQTVTTATTVVTKATW 188

Query: 190 -SKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRERSLQSRRGG--- 249
            S          K E                   S+D+   ++KLLRER +    GG   
Sbjct: 189 FSSGSTSASATFKEEMS-----------------SSDEMMTKKKLLRERLVVPNGGGRRD 248

Query: 250 --------GGG----EVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGN-Y 309
                   GGG    E  EKERY+WGD  RPKVLE+FICN++ A +LKE+V+EKGCG+ Y
Sbjct: 249 SGGGSGSSGGGVVVVEEMEKERYSWGDSCRPKVLEDFICNKKTAIELKEMVKEKGCGHYY 308

Query: 310 IFEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVN 369
           IFEG PGVGKRTMI+AMLR+AFG+Q+MEIKEVV+VFDLKSEM+G IEVKV+ESSHYVEVN
Sbjct: 309 IFEGAPGVGKRTMIQAMLRQAFGNQSMEIKEVVKVFDLKSEMLGSIEVKVKESSHYVEVN 368

Query: 370 LSQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCS 429
           +SQTKGFEKQVIVQL+KE+HSPLPCNH+NC+GILLCEA++LS ETLMY+KWA+ER KGCS
Sbjct: 369 MSQTKGFEKQVIVQLMKESHSPLPCNHANCRGILLCEADQLSIETLMYIKWAMERYKGCS 428

Query: 430 KIFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSK 489
           KIFFCCSD SKLL L+SLCTL+ LSPPSKQEIVEVLEF+AKQ+GFDLS RLAERIADNS+
Sbjct: 429 KIFFCCSDASKLLLLSSLCTLVRLSPPSKQEIVEVLEFIAKQQGFDLSKRLAERIADNSR 488

Query: 490 NNLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK 549
           NNLRQAIRSLEASW KSQLF+EDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK
Sbjct: 489 NNLRQAIRSLEASWKKSQLFDEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK 548

Query: 550 LIEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAK 606
           LIE+DVSPNFIFRTLVDELKKFLDEELQRR+EG YADYNK+EEK FVS ++G+GEEAV K
Sbjct: 549 LIEYDVSPNFIFRTLVDELKKFLDEELQRRVEGFYADYNKLEEKVFVS-EKGSGEEAVIK 594

BLAST of MS005701.1 vs. ExPASy Swiss-Prot
Match: Q8VXX4 (Replication factor C subunit 3 OS=Arabidopsis thaliana OX=3702 GN=RFC3 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 5.7e-43
Identity = 113/315 (35.87%), Postives = 189/315 (60.00%), Query Frame = 0

Query: 254 WGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYIFEGPPGVGKRTMIRAMLREAFGHQ 313
           W DKYRPK L++ I +   A++LK++V E+ C + +F GP G GK+T+I A+L++ +G  
Sbjct: 3   WVDKYRPKSLDKVIVHEDIAQKLKKLVSEQDCPHLLFYGPSGSGKKTLIMALLKQIYGAS 62

Query: 314 AMEIKEVVRVF--DLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQ-LIKETHSP 373
           A ++K   R +  D  S  +  +E+    S+++VE+  S   GF+ + IVQ +IKE    
Sbjct: 63  AEKVKVENRAWKVDAGSRTID-LELTTLSSTNHVELTPSDA-GFQDRYIVQEIIKEMAKN 122

Query: 374 LPCNHSNCQG---ILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLL-PLTSL 433
            P +    +G   ++L E +KLS E    ++  +E+     ++  CC+  SK+   + S 
Sbjct: 123 RPIDTKGKKGYKVLVLNEVDKLSREAQHSLRRTMEKYSSSCRLILCCNSSSKVTEAIKSR 182

Query: 434 CTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQ 493
           C  + ++ PS++EIV+VLEFVAK+E   L    A RIA+ S  +LR+AI SLE    + Q
Sbjct: 183 CLNVRINAPSQEEIVKVLEFVAKKESLQLPQGFAARIAEKSNRSLRRAILSLETC--RVQ 242

Query: 494 LFEEDENQLLT--GWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLV 553
            +    NQ+++   WE+ +A++A  +++EQSPK+L+ VRGK+ +L+ + + P  I + L+
Sbjct: 243 NYPFTGNQVISPMDWEEYVAEIATDMMKEQSPKKLFQVRGKVYELLVNCIPPEVILKRLL 302

Query: 554 DELKKFLDEELQRRI 560
            EL K LD EL+  +
Sbjct: 303 HELLKKLDSELKLEV 313

BLAST of MS005701.1 vs. ExPASy Swiss-Prot
Match: Q852K3 (Replication factor C subunit 5 OS=Oryza sativa subsp. japonica OX=39947 GN=RFC5 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.9e-39
Identity = 101/312 (32.37%), Postives = 175/312 (56.09%), Query Frame = 0

Query: 254 WGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYIFEGPPGVGKRTMIRAMLREAFGHQ 313
           W DKYRPK L++   + + A+ LK++V E+ C + +F GP G GK+T++ A++++ FG  
Sbjct: 3   WVDKYRPKTLDKVTVHDQVAQNLKKLVAEQDCPHLLFYGPSGSGKKTLVMALIKQMFGAG 62

Query: 314 AMEIKEVVRVF--DLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQLIKETHSPL 373
           A ++K   + +  D  S  +  IE+ +  S+H+VE+N S     ++ V+ ++IKE     
Sbjct: 63  ADKVKMENKTWKIDTGSRNI-EIELAMLSSAHHVEMNPSDAGFQDRYVVQEVIKEMAKNR 122

Query: 374 PCNHSNCQG---ILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLL-PLTSLC 433
           P +    +    ++L E +KLS E    ++  +E+     ++  CC+  SK+   + S C
Sbjct: 123 PIDAKGKRAFKVLVLNEVDKLSREAQHSLRRTMEKYSASCRLILCCNSSSKVTEAVRSRC 182

Query: 434 TLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQL 493
             + ++ PS+ +IV+VLEF+ K+E   L    A RIA  S  NLR+AI   E    +   
Sbjct: 183 LNVRVNAPSEDQIVQVLEFIGKKENLQLPFGFAARIAAQSNRNLRRAILFFETCKVQQYP 242

Query: 494 FEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDEL 553
           F  ++      WE  ++++A  I++EQSPK+L+ VR K  +L+ + + P  I + L+ EL
Sbjct: 243 FTSNQVAPPLDWEQYVSEIAADIMKEQSPKRLFAVRQKFYELLVNCIPPESILKKLLAEL 302

Query: 554 KKFLDEELQRRI 560
            K LD +L+  I
Sbjct: 303 LKKLDSDLKHEI 313

BLAST of MS005701.1 vs. ExPASy Swiss-Prot
Match: Q54BN3 (Probable replication factor C subunit 3 OS=Dictyostelium discoideum OX=44689 GN=rfc3 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.8e-37
Identity = 101/310 (32.58%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 254 WGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYIFEGPPGVGKRTMIRAMLREAFGHQ 313
           W DKY+P  L++   +   +  LK +++     + +  GP G GK+T I A+L+E +G  
Sbjct: 3   WIDKYKPTSLDKMDYHNDISINLKNMIKSGDFPHLLVYGPSGAGKKTRILAVLQEIYGPN 62

Query: 314 AMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQLIKETHSPLPC 373
           A+++K   R F   +     I++    S +++E+N  +   +++ VI  +IKE     P 
Sbjct: 63  ALKLKIDHRTFKHPTSS-KNIQITTISSHYHIEINPGEAGSYDRVVIQTIIKEIAQSPPI 122

Query: 374 NHSNCQG---ILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLL-PLTSLCTL 433
           +  +      ++L E +KLS +    ++  +E+     ++  CC   +K++ P+ S C  
Sbjct: 123 DSGSLGAFKIVILNEVDKLSKDAQHALRRTMEKYATFCRLILCCDSTAKVIDPIKSRCLG 182

Query: 434 IHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQLFE 493
           I +  PS++EI +VL  VA  E FDL ++LA  +A  S  NLR A+  LE+   K   F+
Sbjct: 183 IRVPAPSQEEIEKVLAKVATAEKFDLPSKLAVNVAKQSGGNLRYALMLLESQKAKQYPFQ 242

Query: 494 EDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDELKK 553
             E  LL  WE+ I+ + K   EEQSP +L IVRGKL +L+ H + P  IF+TL+ E+ K
Sbjct: 243 STELPLL-DWENYISQIVKDCFEEQSPARLSIVRGKLYELLGHCIPPELIFKTLLLEIFK 302

Query: 554 FLDEELQRRI 560
            LD  ++  I
Sbjct: 303 KLDHNMKFEI 310

BLAST of MS005701.1 vs. ExPASy Swiss-Prot
Match: Q8R323 (Replication factor C subunit 3 OS=Mus musculus OX=10090 GN=Rfc3 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.2e-34
Identity = 95/320 (29.69%), Postives = 171/320 (53.44%), Query Frame = 0

Query: 254 WGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNY---IFEGPPGVGKRTMIRAMLREAF 313
           W DKYRP  L     ++ +A QL+ +V+   CG++   +  GP G GK+T I  +LRE +
Sbjct: 4   WVDKYRPSSLARLDYHKEQAAQLRNLVQ---CGDFPHLLVYGPSGAGKKTRIMCILRELY 63

Query: 314 GHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQLIK---ET 373
           G    +++   +     S+   +IE+    S++++EVN S     ++ VI +++K   ++
Sbjct: 64  GIGVEKLRIEHQTITTPSKK--KIEISTIASNYHLEVNPSDAGNSDRVVIQEMLKTVAQS 123

Query: 374 HSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLL-PLTSL 433
                 +  + + +LL E +KL+ +    ++  +E+     ++  CC+  SK++ P+ S 
Sbjct: 124 QQLETSSQRDFKVVLLTEVDKLTKDAQHALRRTMEKYMSTCRLILCCNSTSKVIPPIRSR 183

Query: 434 CTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQ 493
           C  + +  PS ++I  VL  V ++EG  L + LA R+A+ S  NLR+A+   EA   +  
Sbjct: 184 CLAVRVPAPSIEDICSVLSTVCRKEGLALPSTLARRLAEKSCRNLRKALLMCEACRVQQY 243

Query: 494 LFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDE 553
            F ED+    T WE  + + A  IV +Q+P++L  VRG+L +L+ H + P  I + L+ E
Sbjct: 244 PFTEDQEIPETDWEVYLRETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSE 303

Query: 554 LKKFLDEELQRRIEGLYADY 567
           L    D +L+  +  + A Y
Sbjct: 304 LLHNCDGQLKGEVAQMAAYY 318

BLAST of MS005701.1 vs. ExPASy Swiss-Prot
Match: Q2TBV1 (Replication factor C subunit 3 OS=Bos taurus OX=9913 GN=RFC3 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.9e-34
Identity = 95/320 (29.69%), Postives = 172/320 (53.75%), Query Frame = 0

Query: 254 WGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNY---IFEGPPGVGKRTMIRAMLREAF 313
           W DKYRP  L +   ++ +A QL+ +V+   CG++   +  GP G GK+T I  +LRE +
Sbjct: 4   WVDKYRPCSLGQLDYHKEQAAQLRNLVQ---CGDFPHLLVYGPSGAGKKTRIMCILRELY 63

Query: 314 GHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQLIK---ET 373
           G    +++   +     S+   +IE+    S++++EVN S     ++ VI +++K   ++
Sbjct: 64  GVGVEKLRIEHQTITTPSKK--KIEISTIASNYHLEVNPSDAGNSDRVVIQEMLKTVAQS 123

Query: 374 HSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLL-PLTSL 433
                 +  + + +LL E +KL+ +    ++  +E+     ++  CC+  SK++ P+ S 
Sbjct: 124 QQLETSSQKDFKVVLLTEVDKLTKDAQHALRRTMEKYMSTCRLILCCNSTSKVIPPIRSR 183

Query: 434 CTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQ 493
           C  + +  PS ++I  VL  V K+EG +L  +LA R+A+ S  NLR+A+   EA   +  
Sbjct: 184 CLAVRVPAPSIEDICHVLSTVCKKEGLNLPPQLAHRLAEKSCRNLRKALLMCEACRVQQY 243

Query: 494 LFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDE 553
            F  D+    T WE  + + A  IV +Q+P++L  VRG+L +L+ H + P  I + L+ E
Sbjct: 244 PFTADQEIPETDWEVYLRETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSE 303

Query: 554 LKKFLDEELQRRIEGLYADY 567
           L    D +L+  +  + A Y
Sbjct: 304 LLHNCDGQLKGEVAQMAAYY 318

BLAST of MS005701.1 vs. ExPASy TrEMBL
Match: A0A6J1G337 (replication factor C subunit 3-like OS=Cucurbita moschata OX=3662 GN=LOC111450354 PE=4 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.8e-212
Identity = 423/616 (68.67%), Postives = 478/616 (77.60%), Query Frame = 0

Query: 5   DNNNNPSLRRSVSDPRLSRHRRRRRTFST--TSSKSSWSSKLAKILARLALFSSDSELTE 64
           +++++ SLRRS+SD    R +RRR + ST  +SSKSSWS+KLAK+LARL LFS +S+LTE
Sbjct: 9   NSSSSSSLRRSLSDSNTRRRQRRRGSISTASSSSKSSWSTKLAKLLARLNLFSRNSDLTE 68

Query: 65  ESLEAHNKRIREELEQRQLHDKTP-KSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSG 124
           ESL+AHN+RI +        DKTP KSSPYYRGLTDSSLAINYHHGPL T         G
Sbjct: 69  ESLQAHNERIDD-------LDKTPLKSSPYYRGLTDSSLAINYHHGPLTT---------G 128

Query: 125 PHYASSSATQV---TSFSSIVVSKLKGWAPCL-GKSNDKPAPGPDKNNNNNSIIQNIPTV 184
           PH+ +SS T     TS S  VVSKLK WAPCL GK N KP           + +  +   
Sbjct: 129 PHHYASSTTTAQTQTSLSYAVVSKLKEWAPCLIGKPNTKPPAHQQYETPTTTTV--VGKT 188

Query: 185 TKTASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRERSLQ- 244
           T  A SSS                    PE + R            + +EKLLRER +  
Sbjct: 189 TSLAVSSS--------------------PEKIERWAT---------EKEEKLLRERLVMY 248

Query: 245 -SRRGG------GGGEVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYI 304
             R GG      GGGE AEKERYTWGDKYRPKVLE+FICNR+ A +LK+VV EKGCG+YI
Sbjct: 249 GRREGGSGVGVDGGGEAAEKERYTWGDKYRPKVLEDFICNRKTACELKKVVEEKGCGHYI 308

Query: 305 FEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNL 364
           FEGPPGVGKRTMI+AMLR+AFG QAME+KEV RVF LKSEMVG IEVKV+ESSH VEVNL
Sbjct: 309 FEGPPGVGKRTMIQAMLRQAFGEQAMEVKEVDRVFALKSEMVGSIEVKVKESSHCVEVNL 368

Query: 365 SQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSK 424
           SQTKGFEKQVI QLIKET SPLPCNH+ C+GILLCEA++LSNETLMYVKWAIERNKGCSK
Sbjct: 369 SQTKGFEKQVIDQLIKETQSPLPCNHARCRGILLCEADQLSNETLMYVKWAIERNKGCSK 428

Query: 425 IFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKN 484
           IFFCCSDVSKLL L+S CT +HLSPPSKQEIVEVLE++AKQ+ FDLS R+AERIADNSKN
Sbjct: 429 IFFCCSDVSKLLLLSSFCTHVHLSPPSKQEIVEVLEYIAKQQEFDLSRRMAERIADNSKN 488

Query: 485 NLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL 544
           NLRQAIRSLEASW KS+LFEEDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL
Sbjct: 489 NLRQAIRSLEASWKKSRLFEEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKL 548

Query: 545 IEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAKV 604
           IE+DVSPNFIF+TLVDELKKFLDEELQ R+E  YADYNK+EEK FV+ ++GNGEEAV K 
Sbjct: 549 IEYDVSPNFIFKTLVDELKKFLDEELQHRVESFYADYNKMEEKPFVT-EKGNGEEAVIKE 576

Query: 605 LHDPMRKNVHHFMKIE 606
           LHDPMRKNV+HF+KIE
Sbjct: 609 LHDPMRKNVNHFLKIE 576

BLAST of MS005701.1 vs. ExPASy TrEMBL
Match: A0A6J1IEE5 (replication factor C subunit 3-like OS=Cucurbita maxima OX=3661 GN=LOC111474906 PE=4 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 1.1e-211
Identity = 425/617 (68.88%), Postives = 480/617 (77.80%), Query Frame = 0

Query: 7   NNNPSLRRSVSDPRLSRHRRRRRTFST--TSSKSSWSSKLAKILARLALFSSDSELTEES 66
           +++ SLRRS+SD    R +RR  + ST  +SSKSSWS+KLAK+LARL LFS +S+LTEES
Sbjct: 11  SSSSSLRRSLSDSNTRRRQRRHWSISTASSSSKSSWSTKLAKLLARLNLFSRNSDLTEES 70

Query: 67  LEAHNKRIREELEQRQLHDKTP-KSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSGPH 126
           L+AHN+RI +        DKTP KSSPYYRGLTDSSLAINYHHGPL T    H      H
Sbjct: 71  LQAHNERIDD-------LDKTPLKSSPYYRGLTDSSLAINYHHGPLTT---THVTTGPHH 130

Query: 127 YASSSAT--QVTSFSSIVVSKLKGWAPCL-GKSNDKPAPGPDKNNNNNSIIQNIPTVTKT 186
           YASS+ T    TS S  VVSKLK WAPCL GK N KP   P  + +       +   T +
Sbjct: 131 YASSTTTAQTQTSLSYAVVSKLKEWAPCLIGKPNKKP---PAHHQHETPTTTTVVGKTTS 190

Query: 187 ASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRER-SLQSRR 246
            ++SSS                   PE + R            + +EKLLRER  +  RR
Sbjct: 191 LATSSS-------------------PEKIERLAT---------EKEEKLLRERLVMYGRR 250

Query: 247 GG-------GGGEVA----EKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNY 306
            G       GGGE +    EKERYTWGDKYRPKVLE+FICNR+ A +LK+VV EKGCG+Y
Sbjct: 251 EGRSGVGVDGGGEASEKEKEKERYTWGDKYRPKVLEDFICNRQTACELKKVVEEKGCGHY 310

Query: 307 IFEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVN 366
           IFEGPPGVGKRTMI+AMLR+AFG QAME+KEV RVF LKSEMVG IEVKV+ESSH VEVN
Sbjct: 311 IFEGPPGVGKRTMIQAMLRQAFGEQAMEVKEVGRVFALKSEMVGSIEVKVKESSHCVEVN 370

Query: 367 LSQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCS 426
           LSQTKGFEKQVI QLIKET SPLPCNH+ C+GILLCEA++LSNETLMYVKWAIERNKGCS
Sbjct: 371 LSQTKGFEKQVIDQLIKETQSPLPCNHARCRGILLCEADQLSNETLMYVKWAIERNKGCS 430

Query: 427 KIFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSK 486
           KIFFCCSDVSKLL L+SLCT +HLSPPSKQEIVEVLE++AKQ+ FDLS R+AERIADNSK
Sbjct: 431 KIFFCCSDVSKLLLLSSLCTHVHLSPPSKQEIVEVLEYIAKQQEFDLSRRMAERIADNSK 490

Query: 487 NNLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK 546
           NNLRQAIRSLEASW KS+LFEEDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK
Sbjct: 491 NNLRQAIRSLEASWKKSRLFEEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKK 550

Query: 547 LIEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAK 606
           LIE+DVSPNFIF+TLVDELKKFLDEELQRR+E  YADYNK+EEK FV+D +GNGEEAV K
Sbjct: 551 LIEYDVSPNFIFKTLVDELKKFLDEELQRRVESFYADYNKMEEKPFVTD-KGNGEEAVIK 585

BLAST of MS005701.1 vs. ExPASy TrEMBL
Match: A0A1S3AWH3 (replication factor C subunit 3-like OS=Cucumis melo OX=3656 GN=LOC103483394 PE=4 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 1.4e-204
Identity = 412/619 (66.56%), Postives = 476/619 (76.90%), Query Frame = 0

Query: 1   MANDDNNNNPSLRRSVSDPRLSRHR-RRRRTFSTTSSKSSWSSKLAKILARLALFSSDSE 60
           MA       PSLRRS+SD    R R RRR T STTSSKSSWSSKL K+LARLAL S DS+
Sbjct: 1   MATQQQLQQPSLRRSLSDSNHRRRRSRRRATISTTSSKSSWSSKLGKLLARLALLSRDSD 60

Query: 61  LTEESLEAHNKRIREELEQRQLHDKTPKSSPYYRGLTDSSLAINYHHGPLATPGPAHQQL 120
           LTEESLEAHNKRI +        +KTPKSSPYYRGLTDSSLAINYHHGPL          
Sbjct: 61  LTEESLEAHNKRIND----LDTLEKTPKSSPYYRGLTDSSLAINYHHGPLN--------- 120

Query: 121 SGPHYASSSATQVTSFSSIVVSKLKG-WAPCLGKSNDKPAP--------GPDKNNNNNSI 180
           S P+  + + +   S  S +VSK K  WAPCL K   +  P         P ++    ++
Sbjct: 121 STPYNVTYATSAAPSTQSSIVSKFKEYWAPCLRKQQQQQQPPHTTPHKVPPRQHQRIQTV 180

Query: 181 IQNIPTVTKTASSSSSKPKPRPKPRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLL 240
             +   VTK    SS         + +   D     + + R  E L +SN  G+      
Sbjct: 181 TTSTTVVTKATWFSSGSTSASANFKEEMSSDEMMTKKKLLR--ERLVVSN-GGER----- 240

Query: 241 RERSLQSRRGGGG---GEVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGN 300
           R+R   S  GGGG    E  EKERY+WGD YRPK LE+FICN++ A +LKE+V+EKGCG+
Sbjct: 241 RDRGGGSGSGGGGVVVVEEMEKERYSWGDNYRPKFLEDFICNKKTAIELKEMVKEKGCGH 300

Query: 301 -YIFEGPPGVGKRTMIRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVE 360
            YIFEG PGVGKRTMI+AMLREAFG+Q++EIKEVV+VFDLKSEM+G IEVKV+ESSHYVE
Sbjct: 301 YYIFEGAPGVGKRTMIQAMLREAFGNQSVEIKEVVKVFDLKSEMLGSIEVKVKESSHYVE 360

Query: 361 VNLSQTKGFEKQVIVQLIKETHSPLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKG 420
           VN+SQTKGFEKQVIVQL+KE+HSPLPCNH+NC+GILLCEA++LSNETLMYVKWAIERNKG
Sbjct: 361 VNMSQTKGFEKQVIVQLMKESHSPLPCNHANCRGILLCEADQLSNETLMYVKWAIERNKG 420

Query: 421 CSKIFFCCSDVSKLLPLTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADN 480
           C+KIFFCC+D SKL  L+S+CTL+HLSPPSKQEIVEVLEF+AKQ+GFDLS RLAERIADN
Sbjct: 421 CTKIFFCCTDASKLHLLSSVCTLVHLSPPSKQEIVEVLEFIAKQQGFDLSKRLAERIADN 480

Query: 481 SKNNLRQAIRSLEASWNKSQLFEEDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKL 540
           SKNNLRQAIRSLEASW KSQLF+EDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKL
Sbjct: 481 SKNNLRQAIRSLEASWKKSQLFDEDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKL 540

Query: 541 KKLIEHDVSPNFIFRTLVDELKKFLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAV 600
           KKLIE+DVSPNFIFRTLVDELKKFLDEELQRR+EG YADY K+EEK FVS ++G+GEE V
Sbjct: 541 KKLIEYDVSPNFIFRTLVDELKKFLDEELQRRVEGFYADYYKLEEKAFVS-ERGSGEETV 597

Query: 601 AKVLHDPMRKNVHHFMKIE 606
            K  +DP+RKNV+HF+KIE
Sbjct: 601 VKGQNDPVRKNVNHFLKIE 597

BLAST of MS005701.1 vs. ExPASy TrEMBL
Match: A0A5A7TZV7 (Replication factor C subunit 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G003010 PE=4 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 1.3e-202
Identity = 402/597 (67.34%), Postives = 465/597 (77.89%), Query Frame = 0

Query: 23  RHRRRRRTFSTTSSKSSWSSKLAKILARLALFSSDSELTEESLEAHNKRIREELEQRQLH 82
           R  RRR T STTSSKSSWSSKL K+LARLAL S DS+LTEESLEAHNKRI +        
Sbjct: 10  RRSRRRATISTTSSKSSWSSKLGKLLARLALLSRDSDLTEESLEAHNKRIND----LDTL 69

Query: 83  DKTPKSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSGPHYASSSATQVTSFSSIVVSK 142
           +KTPKSSPYYRGLTDSSLAINYHHGPL          S P+  + + +   S  S +VSK
Sbjct: 70  EKTPKSSPYYRGLTDSSLAINYHHGPLN---------STPYNVTYATSAAPSTQSSIVSK 129

Query: 143 LKG-WAPCLGKSNDKPAP--------GPDKNNNNNSIIQNIPTVTKTASSSSSKPKPRPK 202
            K  WAPCL K   +  P         P ++    ++  +   VTK    SS        
Sbjct: 130 FKEYWAPCLRKQQQQQQPPHTTPHKVPPRQHQRIQTVTTSTTVVTKATWFSSGSTSASAN 189

Query: 203 PRPKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRERSLQSRRGGGG---GEVAEKE 262
            + +   D     + + R  E L +SN  G+      R+R   S  GGGG    E  EKE
Sbjct: 190 FKEEMSSDEMMTKKKLLR--ERLVVSN-GGER-----RDRGGGSGSGGGGVVVVEEMEKE 249

Query: 263 RYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGN-YIFEGPPGVGKRTMIRAMLREA 322
           RY+WGD YRPK LE+FICN++ A +LKE+V+EKGCG+ YIFEG PGVGKRTMI+AMLREA
Sbjct: 250 RYSWGDNYRPKFLEDFICNKKTAIELKEMVKEKGCGHYYIFEGAPGVGKRTMIQAMLREA 309

Query: 323 FGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQLIKETHS 382
           FG+Q++EIKEVV+VFDLKSEM+G IEVKV+ESSHYVEVN+SQTKGFEKQVIVQL+KE+HS
Sbjct: 310 FGNQSVEIKEVVKVFDLKSEMLGSIEVKVKESSHYVEVNMSQTKGFEKQVIVQLMKESHS 369

Query: 383 PLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLLPLTSLCTL 442
           PLPCNH+NC+GILLCEA++LSNETLMYVKWAIERNKGC+KIFFCC+D SKL  L+S+CTL
Sbjct: 370 PLPCNHANCRGILLCEADQLSNETLMYVKWAIERNKGCTKIFFCCTDASKLHLLSSVCTL 429

Query: 443 IHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQLFE 502
           +HLSPPSKQEIVEVLEF+AKQ+GFDLS RLAERIADNSKNNLRQAIRSLEASW KSQLF+
Sbjct: 430 VHLSPPSKQEIVEVLEFIAKQQGFDLSKRLAERIADNSKNNLRQAIRSLEASWKKSQLFD 489

Query: 503 EDENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDELKK 562
           EDEN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIE+DVSPNFIFRTLVDELKK
Sbjct: 490 EDENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEYDVSPNFIFRTLVDELKK 549

Query: 563 FLDEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAKVLHDPMRKNVHHFMKIEG 607
           FLDEELQRR+EG YADY K+EEK FVS ++G+GEE V K  +DP+RKNV+HF+KIEG
Sbjct: 550 FLDEELQRRVEGFYADYYKLEEKAFVS-ERGSGEETVVKGQNDPVRKNVNHFLKIEG 584

BLAST of MS005701.1 vs. ExPASy TrEMBL
Match: A0A5D3D1K1 (Replication factor C subunit 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003550 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 1.0e-172
Identity = 345/535 (64.49%), Postives = 405/535 (75.70%), Query Frame = 0

Query: 85  TPKSSPYYRGLTDSSLAINYHHGPLATPGPAHQQLSGPHYASSSATQVTSFSSIVVSKLK 144
           +P  +PYYRGLTDSSLAINYHHGPL          S P+  + + +   S  S +VSK K
Sbjct: 13  SPLVTPYYRGLTDSSLAINYHHGPLN---------STPYNVTYATSAAPSTQSSIVSKFK 72

Query: 145 G-WAPCLGKSNDKPAP--------GPDKNNNNNSIIQNIPTVTKTASSSSSKPKPRPKPR 204
             WAPCL K   +  P         P ++    ++  +   VTK    SS         +
Sbjct: 73  EYWAPCLRKQQQQQQPPHTTPHKVPPRQHQRIQTVTTSTTVVTKATWFSSGSTSASANFK 132

Query: 205 PKPEPDPEPEPEPVRRSEEALNISNDDGDHQEKLLRERSLQSRRGGGG---GEVAEKERY 264
            +   D     + + R  E L +SN  G+      R+R   S  GGGG    E  EKERY
Sbjct: 133 EEMSSDEMMTKKKLLR--ERLVVSN-GGER-----RDRGGGSGSGGGGVVVVEEMEKERY 192

Query: 265 TWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGN-YIFEGPPGVGKRTMIRAMLREAFG 324
           +WGD YRPK LE+FICN++ A +LKE+V+EKGCG+ YIFEG PGVGKRTMI+AMLREAFG
Sbjct: 193 SWGDNYRPKFLEDFICNKKTAIELKEMVKEKGCGHYYIFEGAPGVGKRTMIQAMLREAFG 252

Query: 325 HQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQLIKETHSPL 384
           +Q++E           SEM+G IEVKV+ESSHYVEVN+SQTKGFEKQVIVQL+KE+HSPL
Sbjct: 253 NQSVE-----------SEMLGSIEVKVKESSHYVEVNMSQTKGFEKQVIVQLMKESHSPL 312

Query: 385 PCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLLPLTSLCTLIH 444
           PCNH+NC+GI LCEA++LSNETLMYVKWAIERNKGC+KIFFCC+D SKL  L+S+CTL+H
Sbjct: 313 PCNHANCRGISLCEADQLSNETLMYVKWAIERNKGCTKIFFCCTDASKLHLLSSVCTLVH 372

Query: 445 LSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQLFEED 504
           LSPPSKQEIVEVLEF+AKQ+GFDLS RLAERIADNSKNNLRQAIRSLEASW KSQLF+ED
Sbjct: 373 LSPPSKQEIVEVLEFIAKQQGFDLSKRLAERIADNSKNNLRQAIRSLEASWKKSQLFDED 432

Query: 505 ENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDELKKFL 564
           EN+LLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIE+DVSPNFIFRTLVDELKKFL
Sbjct: 433 ENKLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEYDVSPNFIFRTLVDELKKFL 492

Query: 565 DEELQRRIEGLYADYNKIEEKTFVSDQQGNGEEAVAKVLHDPMRKNVHHFMKIEG 607
           DEELQRR+EG YADY K+EEK FVS ++G+GEE V K  +DP+RKNV+HF+KIEG
Sbjct: 493 DEELQRRVEGFYADYYKLEEKAFVS-ERGSGEETVVKGQNDPVRKNVNHFLKIEG 518

BLAST of MS005701.1 vs. TAIR 10
Match: AT5G27740.1 (ATPase family associated with various cellular activities (AAA) )

HSP 1 Score: 177.2 bits (448), Expect = 4.1e-44
Identity = 113/315 (35.87%), Postives = 189/315 (60.00%), Query Frame = 0

Query: 254 WGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYIFEGPPGVGKRTMIRAMLREAFGHQ 313
           W DKYRPK L++ I +   A++LK++V E+ C + +F GP G GK+T+I A+L++ +G  
Sbjct: 3   WVDKYRPKSLDKVIVHEDIAQKLKKLVSEQDCPHLLFYGPSGSGKKTLIMALLKQIYGAS 62

Query: 314 AMEIKEVVRVF--DLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIVQ-LIKETHSP 373
           A ++K   R +  D  S  +  +E+    S+++VE+  S   GF+ + IVQ +IKE    
Sbjct: 63  AEKVKVENRAWKVDAGSRTID-LELTTLSSTNHVELTPSDA-GFQDRYIVQEIIKEMAKN 122

Query: 374 LPCNHSNCQG---ILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLL-PLTSL 433
            P +    +G   ++L E +KLS E    ++  +E+     ++  CC+  SK+   + S 
Sbjct: 123 RPIDTKGKKGYKVLVLNEVDKLSREAQHSLRRTMEKYSSSCRLILCCNSSSKVTEAIKSR 182

Query: 434 CTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQ 493
           C  + ++ PS++EIV+VLEFVAK+E   L    A RIA+ S  +LR+AI SLE    + Q
Sbjct: 183 CLNVRINAPSQEEIVKVLEFVAKKESLQLPQGFAARIAEKSNRSLRRAILSLETC--RVQ 242

Query: 494 LFEEDENQLLT--GWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLV 553
            +    NQ+++   WE+ +A++A  +++EQSPK+L+ VRGK+ +L+ + + P  I + L+
Sbjct: 243 NYPFTGNQVISPMDWEEYVAEIATDMMKEQSPKKLFQVRGKVYELLVNCIPPEVILKRLL 302

Query: 554 DELKKFLDEELQRRI 560
            EL K LD EL+  +
Sbjct: 303 HELLKKLDSELKLEV 313

BLAST of MS005701.1 vs. TAIR 10
Match: AT1G63160.1 (replication factor C 2 )

HSP 1 Score: 69.7 bits (169), Expect = 9.2e-12
Identity = 73/300 (24.33%), Postives = 124/300 (41.33%), Query Frame = 0

Query: 254 WGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYIFEGPPGVGKRTMIRAMLREAFGHQ 313
           W +KYRP  + + + N     +L+ + R+    N I  GPPG GK T I A+  E  G  
Sbjct: 17  WVEKYRPSKVVDIVGNEDAVSRLQVIARDGNMPNLILSGPPGTGKTTSILALAHELLG-- 76

Query: 314 AMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFE--KQVIVQLIKETHSPL 373
               KE V                       +E+N S  +G +  +  I    ++  +  
Sbjct: 77  -TNYKEAV-----------------------LELNASDDRGIDVVRNKIKMFAQKKVTLP 136

Query: 374 PCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSKLL-PLTSLCTLI 433
           P  H   + ++L EA+ +++     ++  IE     ++    C+  +K++ P+ S C L+
Sbjct: 137 PGRH---KVVILDEADSMTSGAQQALRRTIEIYSNSTRFALACNTSAKIIEPIQSRCALV 196

Query: 434 HLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSLEASWNKSQLFEE 493
             S  S Q+I+  L  V   E         E I   +  ++RQA+ +L+A+++    F  
Sbjct: 197 RFSRLSDQQILGRLLVVVAAEKVPYVPEGLEAIIFTADGDMRQALNNLQATFSGFS-FVN 256

Query: 494 DENQLLTGWEDDIADVAKKIVEEQSPKQLYIVRGKLKKLIEHDVSPNFIFRTLVDELKKF 551
            EN      +     V K IV      +  I    LK+L +   SP  I  TL   +K +
Sbjct: 257 QENVFKVCDQPHPLHV-KNIVRNVLESKFDIACDGLKQLYDLGYSPTDIITTLFRIIKNY 285

BLAST of MS005701.1 vs. TAIR 10
Match: AT1G77470.1 (replication factor C subunit 3 )

HSP 1 Score: 61.2 bits (147), Expect = 3.3e-09
Identity = 57/265 (21.51%), Postives = 118/265 (44.53%), Query Frame = 0

Query: 242 GGGEVAEKERYTWGDKYRPKVLEEFICNRRKARQLKEVVREKGCGNYIFEGPPGVGKRTM 301
           G G   + +   W +KYRP+ L++   +R     +  +  E    + +  GPPG GK + 
Sbjct: 29  GFGPPPQSKATPWVEKYRPQSLDDVAAHRDIIDTIDRLTNENKLPHLLLYGPPGTGKTST 88

Query: 302 IRAMLREAFGHQAMEIKEVVRVFDLKSEMVGRIEVKVRESSHYVEVNLSQTKGFEKQVIV 361
           I A+ R+ +G +   +                           +E+N S  +G +  V+ 
Sbjct: 89  ILAVARKLYGPKYRNM--------------------------ILELNASDDRGID--VVR 148

Query: 362 QLIKETHS--PLPCNHSNCQGILLCEAEKLSNETLMYVKWAIERNKGCSKIFFCCSDVSK 421
           Q I++  S        S+ + +LL EA+ ++ +    ++  IE+    ++     + V+K
Sbjct: 149 QQIQDFASTQSFSLGKSSVKLVLLDEADAMTKDAQFALRRVIEKYTKSTRFALIGNHVNK 208

Query: 422 LLP-LTSLCTLIHLSPPSKQEIVEVLEFVAKQEGFDLSTRLAERIADNSKNNLRQAIRSL 481
           ++P L S CT    +P     + + L+ V + E   +S      +   S  ++R+A+  L
Sbjct: 209 IIPALQSRCTRFRFAPLDGVHMSQRLKHVIEAERLVVSDCGLAALVRLSNGDMRKALNIL 263

Query: 482 EASWNKSQLFEEDENQLLTGWEDDI 504
           +++   S+   E+E++ +T  E+D+
Sbjct: 269 QSTHMASKEITEEESKQIT--EEDV 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6597225.12.1e-21268.67Replication factor C subunit 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022946202.13.6e-21268.67replication factor C subunit 3-like [Cucurbita moschata][more]
XP_022975501.12.4e-21168.88replication factor C subunit 3-like [Cucurbita maxima][more]
XP_008438224.12.8e-20466.56PREDICTED: replication factor C subunit 3-like [Cucumis melo][more]
XP_011650816.12.8e-20466.61replication factor C subunit 3 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q8VXX45.7e-4335.87Replication factor C subunit 3 OS=Arabidopsis thaliana OX=3702 GN=RFC3 PE=2 SV=1[more]
Q852K32.9e-3932.37Replication factor C subunit 5 OS=Oryza sativa subsp. japonica OX=39947 GN=RFC5 ... [more]
Q54BN32.8e-3732.58Probable replication factor C subunit 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
Q8R3232.2e-3429.69Replication factor C subunit 3 OS=Mus musculus OX=10090 GN=Rfc3 PE=1 SV=1[more]
Q2TBV12.9e-3429.69Replication factor C subunit 3 OS=Bos taurus OX=9913 GN=RFC3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G3371.8e-21268.67replication factor C subunit 3-like OS=Cucurbita moschata OX=3662 GN=LOC11145035... [more]
A0A6J1IEE51.1e-21168.88replication factor C subunit 3-like OS=Cucurbita maxima OX=3661 GN=LOC111474906 ... [more]
A0A1S3AWH31.4e-20466.56replication factor C subunit 3-like OS=Cucumis melo OX=3656 GN=LOC103483394 PE=4... [more]
A0A5A7TZV71.3e-20267.34Replication factor C subunit 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3D1K11.0e-17264.49Replication factor C subunit 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT5G27740.14.1e-4435.87ATPase family associated with various cellular activities (AAA) [more]
AT1G63160.19.2e-1224.33replication factor C 2 [more]
AT1G77470.13.3e-0921.51replication factor C subunit 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 552..572
NoneNo IPR availableGENE3D1.10.8.60coord: 436..491
e-value: 6.6E-13
score: 50.0
NoneNo IPR availableGENE3D1.20.272.10coord: 498..608
e-value: 2.8E-21
score: 77.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..246
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 159..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 192..235
NoneNo IPR availablePANTHERPTHR11669REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNITcoord: 79..608
NoneNo IPR availablePANTHERPTHR11669:SF52OS10G0574500 PROTEINcoord: 79..608
NoneNo IPR availableCDDcd00009AAAcoord: 274..340
e-value: 6.28276E-4
score: 38.6663
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 252..435
e-value: 6.1E-27
score: 96.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 252..486
IPR008921DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminalSUPERFAMILY48019post-AAA+ oligomerization domain-likecoord: 498..561

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS005701MS005701gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS005701.1-cdsMS005701.1-cds-scaffold254:927660..928607CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:929463..929495CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:929628..929784CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:930372..930550CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:931024..931160CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:931257..931355CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:931458..931527CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:931813..931890CDS
MS005701.1-cdsMS005701.1-cds-scaffold254:932055..932180CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS005701.1MS005701.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006260 DNA replication
molecular_function GO:0003677 DNA binding