MS004046.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS004046.1 vs. NCBI nr
Match: XP_022136978.1 (peptidyl-tRNA hydrolase, chloroplastic [Momordica charantia])

HSP 1 Score: 517.7 bits (1332), Expect = 5.8e-143
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLRIFSSMSTSSPPTADQLAVDDKK 60
           MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLRIFSSMSTSSPPTADQLAVDDKK
Sbjct: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLRIFSSMSTSSPPTADQLAVDDKK 60

Query: 61  PSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDV 120
           PSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDV
Sbjct: 61  PSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDV 120

Query: 121 PIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGM 180
           PIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGM
Sbjct: 121 PIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGM 180

Query: 181 RSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILL 240
           RSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILL
Sbjct: 181 RSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILL 240

Query: 241 LEGFNKSATFVNTNKPLEQI 261
           LEGFNKSATFVNTNKPLEQI
Sbjct: 241 LEGFNKSATFVNTNKPLEQI 260

BLAST of MS004046.1 vs. NCBI nr
Match: XP_038893864.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893865.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893866.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 468.0 bits (1203), Expect = 5.3e-128
Identity = 240/274 (87.59%), Postives = 246/274 (89.78%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFP-----NSGSLRIFS---------SMSTS 60
           MN+G SISS+ ALRLPI LHCFRTSK L SFP     NSG+  IFS         SMSTS
Sbjct: 1   MNVGSSISSVTALRLPICLHCFRTSKTLISFPSLSFLNSGTSHIFSSMTSSSITASMSTS 60

Query: 61  SPPTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSV 120
           S PT DQL VDDKKP+PPPKPWLIVGLGNPGKKYD TRHNVGFEMVDTVAEAEGISMSSV
Sbjct: 61  SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDTVAEAEGISMSSV 120

Query: 121 SFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKL 180
           SFKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KL
Sbjct: 121 SFKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKL 180

Query: 181 RMLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELN 240
           RMLPKGGHGG NGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELN
Sbjct: 181 RMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELN 240

Query: 241 FTLQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           FTLQVG +AIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 FTLQVGTDAIRILLLEGFNKSATFVNTNKPLEQI 274

BLAST of MS004046.1 vs. NCBI nr
Match: XP_008457243.1 (PREDICTED: peptidyl-tRNA hydrolase, chloroplastic [Cucumis melo])

HSP 1 Score: 451.1 bits (1159), Expect = 6.7e-123
Identity = 229/273 (83.88%), Postives = 240/273 (87.91%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLR-------------IFSSMSTSS 60
           MNIG SISS+AALRLP  +H FRTS+ L SFP+   L              I +SMSTSS
Sbjct: 1   MNIGASISSVAALRLPFSIHSFRTSRTLISFPSLSLLHSPTFHNFSSITSSITASMSTSS 60

Query: 61  PPTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVS 120
            PT DQ A+DDKKP+PPPKPWLIVGLGNPGKKYD TRHNVGFEMVD VAEAEGIS+S+VS
Sbjct: 61  SPTTDQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDAVAEAEGISISNVS 120

Query: 121 FKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLR 180
           FKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KLR
Sbjct: 121 FKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180

Query: 181 MLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240
           MLPKGGHGG NGMRSIIDH KGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF
Sbjct: 181 MLPKGGHGGHNGMRSIIDHFKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240

Query: 241 TLQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           TLQVG +AIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 TLQVGADAIRILLLEGFNKSATFVNTNKPLEQI 273

BLAST of MS004046.1 vs. NCBI nr
Match: XP_023541955.1 (peptidyl-tRNA hydrolase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.7 bits (1153), Expect = 3.3e-122
Identity = 228/272 (83.82%), Postives = 242/272 (88.97%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFP----NSGSLRIF--------SSMSTSSP 60
           M+ G S+SS+AALR PI LHCFRT +   SFP    N GSL IF        +SMSTSSP
Sbjct: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60

Query: 61  PTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSF 120
              DQLAV++KKP+PPPKPWLIVGLGNPGKKY+ TRHNVGFEMVD VAE EGISMSSVSF
Sbjct: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRM 180
           KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KLRM
Sbjct: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFT 240
           LPKGGHGG NGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFV+RPFNKQEREEL+FT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 LQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           +QVG++AIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 272

BLAST of MS004046.1 vs. NCBI nr
Match: XP_004137468.1 (peptidyl-tRNA hydrolase, chloroplastic [Cucumis sativus] >KGN64111.1 hypothetical protein Csa_014269 [Cucumis sativus])

HSP 1 Score: 444.9 bits (1143), Expect = 4.8e-121
Identity = 229/273 (83.88%), Postives = 239/273 (87.55%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFP-----NSGSLRIFS--------SMSTSS 60
           M IG SISS+AALRLP   HCF TSK L SFP     +S +  IFS        SMSTSS
Sbjct: 1   MIIGASISSVAALRLPFSFHCFPTSKTLISFPSLSLLHSPTFHIFSSITSSITASMSTSS 60

Query: 61  PPTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVS 120
            PT +Q A+DDKKP+PPPKPWLIVGLGNPGKKYD TRHNVGFEMVD VAEAEGIS+S+VS
Sbjct: 61  SPTTEQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDAVAEAEGISISNVS 120

Query: 121 FKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLR 180
           FKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KLR
Sbjct: 121 FKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180

Query: 181 MLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240
           MLPKGGHGG NGMRSIIDH KGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF
Sbjct: 181 MLPKGGHGGHNGMRSIIDHFKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240

Query: 241 TLQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           TLQVG +AIRILLLEGFNKSATFVNTNK LEQI
Sbjct: 241 TLQVGEDAIRILLLEGFNKSATFVNTNKSLEQI 273

BLAST of MS004046.1 vs. ExPASy Swiss-Prot
Match: Q8GW64 (Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g18440 PE=2 SV=2)

HSP 1 Score: 352.4 bits (903), Expect = 4.2e-96
Identity = 176/224 (78.57%), Postives = 195/224 (87.05%), Query Frame = 0

Query: 40  FSSMSTSSPPTADQL-AVDDKKPSPPP--KPWLIVGLGNPGKKYDGTRHNVGFEMVDTVA 99
           FSS S SS P ++ L A+   KP  PP   PWLIVGLGNPGKKY GTRHNVGFEMVD +A
Sbjct: 64  FSSNSFSSQPESELLQALPSSKPKSPPLQLPWLIVGLGNPGKKYQGTRHNVGFEMVDALA 123

Query: 100 EAEGISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIF 159
           +AEGISM++V+FKAL GKG IG++PIMLAKPQTFMN SGESVG IVS+YKIPLKQVLV++
Sbjct: 124 DAEGISMNTVNFKALFGKGVIGNIPIMLAKPQTFMNLSGESVGQIVSFYKIPLKQVLVVY 183

Query: 160 DDLDLPFGKLRMLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRP 219
           DDLDLPFGKLR+LPKGGHGG NGMRSIID LKGSRDFPRLRIGIGRPPGKMD ANFVLR 
Sbjct: 184 DDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQ 243

Query: 220 FNKQEREELNFTLQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           FN+QE+EEL+ T Q GLEAIRILLLEGFNKSATFVNT K +EQ+
Sbjct: 244 FNRQEQEELDHTFQTGLEAIRILLLEGFNKSATFVNTRKSMEQL 287

BLAST of MS004046.1 vs. ExPASy Swiss-Prot
Match: Q5N9Q7 (Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0693900 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 3.6e-87
Identity = 150/195 (76.92%), Postives = 173/195 (88.72%), Query Frame = 0

Query: 66  KPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDVPIMLA 125
           KPWL VGLGNPGK Y GTRHNVGFEM+D +AEAEGIS+SS+ FKA++GKG IGD PIMLA
Sbjct: 54  KPWLFVGLGNPGKVYQGTRHNVGFEMIDVIAEAEGISLSSMQFKAMVGKGRIGDAPIMLA 113

Query: 126 KPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGMRSIID 185
           KPQTFMNASGESVG +VSY+KIPL QVLV++DDLDLPF KLR+LPKGGHGG NG+RSII+
Sbjct: 114 KPQTFMNASGESVGQLVSYFKIPLNQVLVMYDDLDLPFAKLRLLPKGGHGGHNGVRSIIN 173

Query: 186 HLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILLLEGFN 245
           HLK +RDFPRLRIGIGRPPGKMD ANFVLRPFN++E+EEL+F    GLEA+RI+ LEGFN
Sbjct: 174 HLKQNRDFPRLRIGIGRPPGKMDPANFVLRPFNRKEQEELDFAFHRGLEAVRIMALEGFN 233

Query: 246 KSATFVNTNKPLEQI 261
           KSAT+VNT +  E +
Sbjct: 234 KSATYVNTAQSSEML 248

BLAST of MS004046.1 vs. ExPASy Swiss-Prot
Match: Q9LF14 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 104/186 (55.91%), Postives = 143/186 (76.88%), Query Frame = 0

Query: 67  PWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDVPIMLAK 126
           PWLIVGLGNPG KY GTRHNVGFEM+D +A  EG+ M+++  KALIG G I DVPI+LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 127 PQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGMRSIIDH 186
           PQT+MN SGESVG++ S+Y++PL+ +L+I+D++ LP G LR+ PKGG G  NG++S++ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 187 LKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILLLEGFNK 246
           L G R+FPRL IGIG+PPG MD   F+L+ F+  E++++   L+ G EA++ L+L GFN+
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 247 SATFVN 253
             +  N
Sbjct: 225 GISRFN 230

BLAST of MS004046.1 vs. ExPASy Swiss-Prot
Match: Q6NLS8 (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 99/195 (50.77%), Postives = 147/195 (75.38%), Query Frame = 0

Query: 66  KPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDVPIMLA 125
           +PWL +GLGNPG KY GTRHN+GFEM+D  AE+ GI M+ V+FKA++G+GF+ D+P++LA
Sbjct: 17  RPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVILA 76

Query: 126 KPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGMRSIID 185
           KPQT+MN SGES G + +YYK+PL +VLV+ DD+ LP G LR+  KGGHG  NG++S+++
Sbjct: 77  KPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMN 136

Query: 186 HLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILLLEGFN 245
           H +G+R+F RLRIGIG+PPG+MD   F+L+ F+   RE ++  L  G++A++++L + F 
Sbjct: 137 HFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFG 196

Query: 246 KSATFVNTNKPLEQI 261
           +S    N  +  + +
Sbjct: 197 ESWRLFNVEQKYKHL 211

BLAST of MS004046.1 vs. ExPASy Swiss-Prot
Match: Q9M5P4 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays OX=4577 GN=CRS2 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.9e-59
Identity = 100/186 (53.76%), Postives = 144/186 (77.42%), Query Frame = 0

Query: 67  PWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDVPIMLAK 126
           PWLI GLGNPG KY GTRHNVGFEMVD +A  EGI+M+++  K+L+G G IG+VP+++ K
Sbjct: 61  PWLIAGLGNPGNKYYGTRHNVGFEMVDRIAAEEGITMNTIQSKSLLGIGSIGEVPVLVVK 120

Query: 127 PQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGMRSIIDH 186
           PQ++MN SGE++G + +YY++PL+ +L+I+DD  LP G LR+  KGGHG  NG++++I+H
Sbjct: 121 PQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEH 180

Query: 187 LKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILLLEGFNK 246
           L G R+FPRL IGIG PPGKMD   F+L+ F+ +ER +++  L+ G++A+R L+L+GF+ 
Sbjct: 181 LDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDTALEQGVDAVRTLVLKGFSG 240

Query: 247 SATFVN 253
           S    N
Sbjct: 241 STERFN 246

BLAST of MS004046.1 vs. ExPASy TrEMBL
Match: A0A6J1C906 (peptidyl-tRNA hydrolase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111008552 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 2.8e-143
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLRIFSSMSTSSPPTADQLAVDDKK 60
           MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLRIFSSMSTSSPPTADQLAVDDKK
Sbjct: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLRIFSSMSTSSPPTADQLAVDDKK 60

Query: 61  PSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDV 120
           PSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDV
Sbjct: 61  PSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDV 120

Query: 121 PIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGM 180
           PIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGM
Sbjct: 121 PIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGM 180

Query: 181 RSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILL 240
           RSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILL
Sbjct: 181 RSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILL 240

Query: 241 LEGFNKSATFVNTNKPLEQI 261
           LEGFNKSATFVNTNKPLEQI
Sbjct: 241 LEGFNKSATFVNTNKPLEQI 260

BLAST of MS004046.1 vs. ExPASy TrEMBL
Match: A0A1S3C5R3 (peptidyl-tRNA hydrolase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496915 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 3.2e-123
Identity = 229/273 (83.88%), Postives = 240/273 (87.91%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFPNSGSLR-------------IFSSMSTSS 60
           MNIG SISS+AALRLP  +H FRTS+ L SFP+   L              I +SMSTSS
Sbjct: 1   MNIGASISSVAALRLPFSIHSFRTSRTLISFPSLSLLHSPTFHNFSSITSSITASMSTSS 60

Query: 61  PPTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVS 120
            PT DQ A+DDKKP+PPPKPWLIVGLGNPGKKYD TRHNVGFEMVD VAEAEGIS+S+VS
Sbjct: 61  SPTTDQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDAVAEAEGISISNVS 120

Query: 121 FKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLR 180
           FKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KLR
Sbjct: 121 FKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180

Query: 181 MLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240
           MLPKGGHGG NGMRSIIDH KGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF
Sbjct: 181 MLPKGGHGGHNGMRSIIDHFKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240

Query: 241 TLQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           TLQVG +AIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 TLQVGADAIRILLLEGFNKSATFVNTNKPLEQI 273

BLAST of MS004046.1 vs. ExPASy TrEMBL
Match: A0A0A0LVQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042230 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.3e-121
Identity = 229/273 (83.88%), Postives = 239/273 (87.55%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFP-----NSGSLRIFS--------SMSTSS 60
           M IG SISS+AALRLP   HCF TSK L SFP     +S +  IFS        SMSTSS
Sbjct: 1   MIIGASISSVAALRLPFSFHCFPTSKTLISFPSLSLLHSPTFHIFSSITSSITASMSTSS 60

Query: 61  PPTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVS 120
            PT +Q A+DDKKP+PPPKPWLIVGLGNPGKKYD TRHNVGFEMVD VAEAEGIS+S+VS
Sbjct: 61  SPTTEQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDAVAEAEGISISNVS 120

Query: 121 FKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLR 180
           FKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KLR
Sbjct: 121 FKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180

Query: 181 MLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240
           MLPKGGHGG NGMRSIIDH KGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF
Sbjct: 181 MLPKGGHGGHNGMRSIIDHFKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240

Query: 241 TLQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           TLQVG +AIRILLLEGFNKSATFVNTNK LEQI
Sbjct: 241 TLQVGEDAIRILLLEGFNKSATFVNTNKSLEQI 273

BLAST of MS004046.1 vs. ExPASy TrEMBL
Match: A0A6J1JW15 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 8.8e-121
Identity = 226/272 (83.09%), Postives = 240/272 (88.24%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFP----NSGSLRIFS--------SMSTSSP 60
           MNI  S+SS+AALRLPI LHCF T +   SFP    N  SL IFS        SMSTSSP
Sbjct: 1   MNIAASLSSLAALRLPICLHCFPTFRTPISFPSLSLNPPSLHIFSSMTSSITASMSTSSP 60

Query: 61  PTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSF 120
              DQLAV++KKP+PPPKPWLIVGLGNPGKKY+ TRHNVGFEM+D VAE EGISMSS SF
Sbjct: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMLDAVAEVEGISMSSASF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRM 180
           KALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KLRM
Sbjct: 121 KALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFT 240
           LPKGGHGG NGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFV+RPFNKQEREEL+FT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 LQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           +QVG++AIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 272

BLAST of MS004046.1 vs. ExPASy TrEMBL
Match: A0A6J1GS97 (peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456993 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 224/272 (82.35%), Postives = 238/272 (87.50%), Query Frame = 0

Query: 1   MNIGVSISSIAALRLPIRLHCFRTSKILTSFP----NSGSLRIFS--------SMSTSSP 60
           MNI  S+SS+AALRLP+ LHCFRT +   SFP    N  SL IFS        SMSTSSP
Sbjct: 1   MNIAASLSSLAALRLPVCLHCFRTFRTPISFPSLSLNPPSLHIFSSMTSSFTASMSTSSP 60

Query: 61  PTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSF 120
              D LAV++ KP+PPPKPWLIVGLGNPGKKY+ TRHNVGFEMVD VA  EGISMSS SF
Sbjct: 61  LAPDLLAVEENKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAVVEGISMSSASF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRM 180
           KALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPF KLRM
Sbjct: 121 KALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFT 240
           LPKGGHGG NGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFV+RPFNKQEREEL+FT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 LQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           +QVG++AIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 272

BLAST of MS004046.1 vs. TAIR 10
Match: AT1G18440.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 352.4 bits (903), Expect = 3.0e-97
Identity = 176/224 (78.57%), Postives = 195/224 (87.05%), Query Frame = 0

Query: 40  FSSMSTSSPPTADQL-AVDDKKPSPPP--KPWLIVGLGNPGKKYDGTRHNVGFEMVDTVA 99
           FSS S SS P ++ L A+   KP  PP   PWLIVGLGNPGKKY GTRHNVGFEMVD +A
Sbjct: 64  FSSNSFSSQPESELLQALPSSKPKSPPLQLPWLIVGLGNPGKKYQGTRHNVGFEMVDALA 123

Query: 100 EAEGISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIF 159
           +AEGISM++V+FKAL GKG IG++PIMLAKPQTFMN SGESVG IVS+YKIPLKQVLV++
Sbjct: 124 DAEGISMNTVNFKALFGKGVIGNIPIMLAKPQTFMNLSGESVGQIVSFYKIPLKQVLVVY 183

Query: 160 DDLDLPFGKLRMLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRP 219
           DDLDLPFGKLR+LPKGGHGG NGMRSIID LKGSRDFPRLRIGIGRPPGKMD ANFVLR 
Sbjct: 184 DDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQ 243

Query: 220 FNKQEREELNFTLQVGLEAIRILLLEGFNKSATFVNTNKPLEQI 261
           FN+QE+EEL+ T Q GLEAIRILLLEGFNKSATFVNT K +EQ+
Sbjct: 244 FNRQEQEELDHTFQTGLEAIRILLLEGFNKSATFVNTRKSMEQL 287

BLAST of MS004046.1 vs. TAIR 10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 231.5 bits (589), Expect = 7.8e-61
Identity = 104/186 (55.91%), Postives = 143/186 (76.88%), Query Frame = 0

Query: 67  PWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDVPIMLAK 126
           PWLIVGLGNPG KY GTRHNVGFEM+D +A  EG+ M+++  KALIG G I DVPI+LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 127 PQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGMRSIIDH 186
           PQT+MN SGESVG++ S+Y++PL+ +L+I+D++ LP G LR+ PKGG G  NG++S++ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 187 LKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILLLEGFNK 246
           L G R+FPRL IGIG+PPG MD   F+L+ F+  E++++   L+ G EA++ L+L GFN+
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 247 SATFVN 253
             +  N
Sbjct: 225 GISRFN 230

BLAST of MS004046.1 vs. TAIR 10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 231.5 bits (589), Expect = 7.8e-61
Identity = 99/195 (50.77%), Postives = 147/195 (75.38%), Query Frame = 0

Query: 66  KPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDVPIMLA 125
           +PWL +GLGNPG KY GTRHN+GFEM+D  AE+ GI M+ V+FKA++G+GF+ D+P++LA
Sbjct: 17  RPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVILA 76

Query: 126 KPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGMRSIID 185
           KPQT+MN SGES G + +YYK+PL +VLV+ DD+ LP G LR+  KGGHG  NG++S+++
Sbjct: 77  KPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMN 136

Query: 186 HLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILLLEGFN 245
           H +G+R+F RLRIGIG+PPG+MD   F+L+ F+   RE ++  L  G++A++++L + F 
Sbjct: 137 HFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFG 196

Query: 246 KSATFVNTNKPLEQI 261
           +S    N  +  + +
Sbjct: 197 ESWRLFNVEQKYKHL 211

BLAST of MS004046.1 vs. TAIR 10
Match: AT5G16140.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 231.5 bits (589), Expect = 7.8e-61
Identity = 104/186 (55.91%), Postives = 143/186 (76.88%), Query Frame = 0

Query: 67  PWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAEGISMSSVSFKALIGKGFIGDVPIMLAK 126
           PWLIVGLGNPG KY GTRHNVGFEM+D +A  EG+ M+++  KALIG G I DVPI+LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 127 PQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFGKLRMLPKGGHGGQNGMRSIIDH 186
           PQT+MN SGESVG++ S+Y++PL+ +L+I+D++ LP G LR+ PKGG G  NG++S++ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 187 LKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNFTLQVGLEAIRILLLEGFNK 246
           L G R+FPRL IGIG+PPG MD   F+L+ F+  E++++   L+ G EA++ L+L GFN+
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 247 SATFVN 253
             +  N
Sbjct: 225 GISRFN 230

BLAST of MS004046.1 vs. TAIR 10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 219.2 bits (557), Expect = 4.0e-57
Identity = 106/213 (49.77%), Postives = 149/213 (69.95%), Query Frame = 0

Query: 40  FSSMSTSSPPTADQLAVDDKKPSPPPKPWLIVGLGNPGKKYDGTRHNVGFEMVDTVAEAE 99
           FSS+  S P +      D+K       PWLIVGLGNPG KY GTRHN+GFEM+D +A A 
Sbjct: 34  FSSLRASLPVS------DNKLLKFEYTPWLIVGLGNPGLKYYGTRHNIGFEMIDHIARAT 93

Query: 100 GISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDL 159
            ISM+++  KAL+G G +G+VPI+L KPQ +MN SGESVG + +YY+IPL+ +L+I+DD+
Sbjct: 94  DISMNTIQSKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDM 153

Query: 160 DLPFGKLRMLPKGGHGGQNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNK 219
            L  G LR+ PKGGH   NG++++ +HL G R +PRL IGIG PPG MD   F+L+ F+ 
Sbjct: 154 GLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSP 213

Query: 220 QEREELNFTLQVGLEAIRILLLEGFNKSATFVN 253
            ER++++  L+ G+E ++ L+ EGF+ S +  N
Sbjct: 214 LERKQMDEGLEQGVEGVKTLVEEGFSDSISRFN 240