MS003767.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS003767.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	acyl-protein thioesterase 2
Location	scaffold127: 299629 .. 302695 (-)
Sequence length	867
RNA-Seq Expression	MS003767.1
Synteny	MS003767.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCTTGCTTGATTGTATCTCTTTCAGCTGGTAAAGCTGCCAAAAAGGCAATTGAGTTTGGTAAAACCTACGTTGTGAGGCCCAAGGGAAAACACCTAGCGACGGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCTCAAGGTAGTCTTCTTTCTTTCTGTTCTTTTCTTTTCTATTTTTTTTTTGAAGGAAGACTGAATGTTTGAATTTTTCCCTTTCTGCATCGGTTGTAAGAGAAATGAGAAGACATTGATTATTTATTTGCCCACAGCCCTTCTGCATTGGCCAGTTCCACTTACCTTCTCAGTTTGTTTACTCTTTATCGAGCTTCCCCTCAGTCGTGTTCCTAATTTCTCTTATGATATTATTTTGTGGTTTTCTGGGTGATGGTAATGATAAAGTTTTTTGGTATAATGCAAGAATAGCTCTTATTTTGTGTATTTTGACTAAAAAAATGAAGACTTTCAGATCTGCATAAGGGGGTGTGGGTAGTTTCTTGACCTTATTCCTTTTGTTCGGTCTAGTTAGAGCTCTTTGGGCAAAAATGTTTCCTTTTATCATTAATGCTAACTGGAGGCCCCTTTTTGTAACCTATTTTGGCTGTGGGTTGGGTCATGTTGTACCCCCCCATCTAACCTGCTATAATTCTCTGTTCCTTCCTGCTACAAAAGAATTGAATTGCTATTTTCTTTGGTGATTATAGTTAGTGGTATATGATGAATGGTGCAGTAGTTAGGAAATAGGGATTTTTAGAAATTTCCACATAGTGTTTCTCATTTGGATGTTGTGCAACATGCTCTCTCATGGTTGGAGCCCGGGGGAGATAGGGGGGACAGGTCATGAATTTCCTTAATATAGTTGGCTATATATCATAGGTTAAATCCACGCATTAATGGAAATCAACTTTCATTGTTTCACATTGTGTTGTAAACAATATGAGGTTTTATCTATTGGGACTTTTTCTTGTCGCAACTCCATGCAGATTTTTTGTTCACCTATAGGGGTTGATGAATTGTGTTATGAAGTTGTTTTCTTTTATTGGAAGTATCAGCATTATCGAAGTGGCTTTATAATCTGACCTTAAACTGTCTGTCACACTTTTAATTTAAGGCTGTATTTTAACTATACCTTGATAGCTGGATTACCATGTCCTGTTCAAACAATAAAAAGTTATTTTATCCCCTCCCCTCGTATTGGTATGATTACGAGAGTGCTAAGGATACTTCTGGTTGATTTTCAATTGAAACTCTAACTGACACACTTCAAGATTATCCTTAGGATGGGCATTCTAAGAATATTAAGTGAAACATTAAATACGCTGAATCTATTTTCCTGTAATGCCTTGCCATTTTATTCAGTATTCAATACAGTCTCATGTTTATAAATGTTTTGATTTTGTCTGACAGCTGGTCGCAGCTCTTGGAGACACTTCCCTTACCAAATGTAAGAATATGAAGCTTATCAAAATATTTAGAGCAATTATAAAGCTATTCAAATTTTATTTTATTCAGTCCCCCTTTTTCTGTCGCTTTTTTTTTTTTAATCAAATGGGTAAAAAGTAGATTGAAAAATTCATTGTTGGAAGAGGAAATCGTTTCTTTTAGTGTTTTGTATAAACTTGGAGTTACTGATGCAGATTAAATGGATATGTCCGACTGCACCTACTCAACCAATAGCTTTATTTGGTGGCTTTCCTTCCACTGCATGTAAGCTTTCCTTCCTCCAATTGGAGCAAAAGCTTCAATGAAAGAGGCAAATCTCACTTGGTTAAACATATTATATCACAGTGTGAAACAAAAGTTATAGTGAGATGTTTTTATTAAAACCAGGGTTTGATGTGGGAAATCTTTCGGAAGATGGTCCAGATGATTTAGAGGGTTTGGATGCTTCAGCTGCACATGTTTCCTATTTGTTGTCGACCGAGCCAGCTGACAGTATGTCTCTCTTGCAATTTTGATGTGATTAGATCCCTTTAATACTAATTATGTACCTTTTCCATTGATTTTGTACTTGGCATGCAACATGTTCTTCAACTAGTTTTGTTTCACATTGCTCTGTTTTTGGATGCTATGCATCTCACCCCGATGATTAAAAGATTTAAATCTCTATTTAGGATTGTTGGCATAATTTTGTTTGCAATCTTTTATTTTTTACAGTTAAGCTTGGCGTAGGGGGCTTCAGTATGGGAGCAGCTACTGCACTTTATTCAGCGACCTGCCATGCAGTGGGAAAATACGGGAACGGAAATCCTTACCCAGCCAATTTGAGTGCAGTTGTTGGGCTAAGTGGCTGGCTTCCTTGTTCAAAGTTGGCTTCTTAATTTTTGTCATCTGGACAGCCTCTGATCCTTTTACAATCAGTATGAAGCTTCATTTAACGAGTTATCATGGTGATTCCAGGACTTTGAAAAGCAAGATGGAAGGGAAGAATGAAAGTGCTAGCGGAGCGGGATCCTTGCCTATTCTGCTATGTCATGGAAAAGGTATTTATAAATTTGTATTTGTTATGCAGAAGATGGTGACTATTTTTGTTACAGAATCCCCTGTTCTGTGCTTGGGCCAAATATTACAATACAAATTTGAGCTCGTTGTGTTGTTCATATGGTTAATATGTTAAGAAGGGATACAATTTTATCTTGATGTCTGTACATAAAAAGATGGCTGTTATTGTCAATGAAATAGAGTTTGAGAAAATAGTTTTTTCATTGTTAATCTTCATGAACTTCCCAACTCCAAGTTATTATAAGCAACCTAGGCTTCAAATTTGGATTCTGACAATGGTTTGGTGTGTAACTCGTTTGCAGTTGATGATGTGGTTCTCTACAATTTTGGAGAGAAATCTTCCGAGGCGCTACGTACGTGCGGATTTCAGGACGTTACATTTAAATCCTACAGCGGGTATGCAATGAGAAGTTTTCGACTCGTGCGAGTTCATCCCTTCTAAATCGCGAAATTTAAGTACATTTTTTTTAAATCGACTCGTGTTTCGGTTGCAGACTTGGCCACTACACAGTTCCTGAAGAGATGGATGAGGTCCGGGCTTGGCTGATTTCTAAGTTGGGGCTA

mRNA sequence

CTTTCTTGCTTGATTGTATCTCTTTCAGCTGGTAAAGCTGCCAAAAAGGCAATTGAGTTTGGTAAAACCTACGTTGTGAGGCCCAAGGGAAAACACCTAGCGACGGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCTCAAGGTACTATTCAAATTTTATTTTATTCAGTCCCCCTTTTTCTGTCGCTTTTTTTTTTTTAATCAAATGGGTAAAAAGTAGATTGAAAAATTCATGTTGGAAGAGGAAATCGTTTCTTTTAGTGTTTTGTATAAACTTGGAGTTACTGATGCAGATTAAATGGATATGTCCGACTGCACCTACTCAACCAATAGCTTTATTTGGTGGCTTTCCTTCCACTGCATGGTTTGATGTGGGAAATCTTTCGGAAGATGGTCCAGATGATTTAGAGGGTTTGGATGCTTCAGCTGCACATGTTTCCTATTTGTTGTCGACCGAGCCAGCTGACATTAAGCTTGGCGTAGGGGGCTTCAGTATGGGAGCAGCTACTGCACTTTATTCAGCGACCTGCCATGCAGTGGGAAAATACGGGAACGGAAATCCTTACCCAGCCAATTTGAGTGCAGTTGTTGGGCTAAGTGGCTGGCTTCCTTGTTCAAAGACTTTGAAAAGCAAGATGGAAGGGAAGAATGAAAGTGCTAGCGGAGCGGGATCCTTGCCTATTCTGCTATGTCATGGAAAAGTTGATGATGTGGTTCTCTACAATTTTGGAGAGAAATCTTCCGAGGCGCTACGTACGTGCGGATTTCAGGACGTTACATTTAAATCCTACAGCGGACTTGGCCACTACACAGTTCCTGAAGAGATGGATGAGGTCCGGGCTTGGCTGATTTCTAAGTTGGGGCTA

Coding sequence (CDS)

CTTTCTTGCTTGATTGTATCTCTTTCAGCTGGTAAAGCTGCCAAAAAGGCAATTGAGTTTGGTAAAACCTACGTTGTGAGGCCCAAGGGAAAACACCTAGCGACGGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCTCAAGGTACTATTCAAATTTTATTTTATTCAGTCCCCCTTTTTCTGTCGCTTTTTTTTTTTTAATCAAATGGGTAAAAAGTAGATTGAAAAATTCATGTTGGAAGAGGAAATCGTTTCTTTTAGTGTTTTGTATAAACTTGGAGTTACTGATGCAGATTAAATGGATATGTCCGACTGCACCTACTCAACCAATAGCTTTATTTGGTGGCTTTCCTTCCACTGCATGGTTTGATGTGGGAAATCTTTCGGAAGATGGTCCAGATGATTTAGAGGGTTTGGATGCTTCAGCTGCACATGTTTCCTATTTGTTGTCGACCGAGCCAGCTGACATTAAGCTTGGCGTAGGGGGCTTCAGTATGGGAGCAGCTACTGCACTTTATTCAGCGACCTGCCATGCAGTGGGAAAATACGGGAACGGAAATCCTTACCCAGCCAATTTGAGTGCAGTTGTTGGGCTAAGTGGCTGGCTTCCTTGTTCAAAGACTTTGAAAAGCAAGATGGAAGGGAAGAATGAAAGTGCTAGCGGAGCGGGATCCTTGCCTATTCTGCTATGTCATGGAAAAGTTGATGATGTGGTTCTCTACAATTTTGGAGAGAAATCTTCCGAGGCGCTACGTACGTGCGGATTTCAGGACGTTACATTTAAATCCTACAGCGGACTTGGCCACTACACAGTTCCTGAAGAGATGGATGAGGTCCGGGCTTGGCTGATTTCTAAGTTGGGGCTA

Protein sequence

LSCLIVSLSAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLIKWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNLSEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSSEALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL

Homology

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BLAST of MS003767.1 vs. NCBI nr
Match: XP_022144920.1 (acyl-protein thioesterase 2 [Momordica charantia] >XP_022144921.1 acyl-protein thioesterase 2 [Momordica charantia])

HSP 1 Score: 459.1 bits (1180), Expect = 2.7e-125
Identity = 232/281 (82.56%), Postives = 236/281 (83.99%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGS +  + +L + P         
Sbjct: 10  SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSSW--SQLLETLP--------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPTQPIALFGGFPSTAWFDVGNL
Sbjct: 70  ---------------------------LPNIKWICPTAPTQPIALFGGFPSTAWFDVGNL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YPANLSAVVGLSGWLPCSKTLKSKMEGKNES SGAGSLPILLCHGKVDDVVLYNFGEKSS
Sbjct: 190 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESTSGAGSLPILLCHGKVDDVVLYNFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL
Sbjct: 250 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 252

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BLAST of MS003767.1 vs. NCBI nr
Match: XP_038879397.1 (acyl-protein thioesterase 2 [Benincasa hispida])

HSP 1 Score: 421.8 bits (1083), Expect = 4.8e-114
Identity = 212/281 (75.44%), Postives = 226/281 (80.43%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           + G+AAKKAIEFGKTYVVRPKGKH ATVVWLHGLGDNGS +  + +L + P         
Sbjct: 10  AGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSW--SQLLETLP--------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+PIALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHV+YLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YPANLSAVVGLSGWLPCSKTLK+KME KN + SG GSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPANLSAVVGLSGWLPCSKTLKTKMEEKNANNSGVGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           EAL +CGF+DVTFKSYSG+GHYTVPEEMDEV AWL SKLGL
Sbjct: 250 EALHSCGFKDVTFKSYSGVGHYTVPEEMDEVCAWLTSKLGL 252

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BLAST of MS003767.1 vs. NCBI nr
Match: XP_022987844.1 (acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022987845.1 acyl-protein thioesterase 2-like [Cucurbita maxima])

HSP 1 Score: 417.5 bits (1072), Expect = 9.1e-113
Identity = 210/281 (74.73%), Postives = 222/281 (79.00%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           S GKAA++AIEFGKTYVVRPKGKH AT+VWLHGLGDNGS  +S  +   P          
Sbjct: 10  SGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSS-WSQLLEMLP---------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+PIALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRPIALFGGFPSTAWFDVKDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHV+YLLSTEP+DIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVAYLLSTEPSDIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YP NLSAVVGLSGWLPCSKTLKSKME KN S SGAGSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPTNLSAVVGLSGWLPCSKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           +AL + GFQDV FKSY+GLGHYTVPEEMDEV AWL SKLGL
Sbjct: 250 QALHSVGFQDVIFKSYNGLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of MS003767.1 vs. NCBI nr
Match: XP_004150012.1 (acyl-protein thioesterase 2 [Cucumis sativus] >XP_031736139.1 acyl-protein thioesterase 2 [Cucumis sativus] >KGN65303.1 hypothetical protein Csa_019754 [Cucumis sativus])

HSP 1 Score: 416.8 bits (1070), Expect = 1.6e-112
Identity = 211/281 (75.09%), Postives = 224/281 (79.72%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           + G+AAKKAIEFGKTYVVRPKGKH ATVVWLHGLGDNGS +  + +L + P         
Sbjct: 10  AGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSW--SQLLETLP--------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+PIALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHV+YLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YPANLSAVVGLSGWLPCSKTLK+ ME KN   SGAGSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           EALR+ GF+DVTFKSY+ LGHYTVPEEMDEV AWL SKLGL
Sbjct: 250 EALRSSGFKDVTFKSYNSLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of MS003767.1 vs. NCBI nr
Match: XP_023515795.1 (acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.0 bits (1068), Expect = 2.7e-112
Identity = 210/281 (74.73%), Postives = 221/281 (78.65%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           S GKAA++AIEFGKTYVVRPKGKH AT+VWLHGLGDNGS  +S  +   P          
Sbjct: 10  SGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSS-WSQLLEMLP---------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+PIALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRPIALFGGFPSTAWFDVKDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SE GPDDLEGLDASAAHV+YLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEGGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YP NLSAVVGLSGWLPCSKTLKSKME KN S SGAGSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPTNLSAVVGLSGWLPCSKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           +AL + GFQDV FKSY+GLGHYTVPEEMDEV AWL SKLGL
Sbjct: 250 QALHSVGFQDVIFKSYNGLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of MS003767.1 vs. ExPASy Swiss-Prot
Match: Q4I8Q4 (Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=FGRAMPH1_01T20223 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.1e-23
Identity = 78/267 (29.21%), Postives = 126/267 (47.19%), Query Frame = 0


Query: 31  KHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLIKWVKSRLKNSCWKRKSFLLVFC 90
           +H ATV+++HGLGD G  +                       S ++N  W+R+       
Sbjct: 16  QHTATVIFVHGLGDTGHGW----------------------ASAVEN--WRRR------- 75

Query: 91  INLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNLSED-----GPDDLEGLDASAAH 150
              + + ++K+I P AP  PI++  G     WFD+  L  D       +D EG+  S  +
Sbjct: 76  ---QKMDEVKFILPHAPQIPISVNMGMRMPGWFDIKQLGGDVDSLIRNEDTEGIKLSQKY 135

Query: 151 VSYLLSTE----PADIKLGVGGFSMGAATALYSA-TCHAVGKYGNGNPYPANLSAVVGLS 210
              L+  E        ++ +GGFS G A +L +  TC             + L  ++GLS
Sbjct: 136 FHNLIQQEIDSGIVPERIVLGGFSQGGAMSLLAGLTC------------TSKLGGILGLS 195

Query: 211 GWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSSEALRTCGFQDVT 270
            WL  SKT    ++  + +       P+++ HG+ D +V    G+ S+E L+  G+ DV 
Sbjct: 196 SWLLLSKTFADMVKPTDANR----QTPVMMFHGEEDPIVPCERGKLSAELLKGLGY-DVA 231

Query: 271 FKSYSGLGHYTVPEEMDEVRAWLISKL 288
           +K+Y G+GH  VPEE+DEV A+L  +L
Sbjct: 256 WKTYPGMGHSAVPEELDEVEAFLRKQL 231

[Close]

BLAST of MS003767.1 vs. ExPASy Swiss-Prot
Match: Q9WTL7 (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-22
Identity = 70/195 (35.90%), Postives = 102/195 (52.31%), Query Frame = 0


Query: 96  LMQIKWICPTAPTQPIALFGGFPSTAWFDVGNLSEDGPDDLEGLDASAAHVSYLLSTE-- 155
           L  +K+ICP AP  P+ L       +WFD+  LS D P+D  G+  +A ++  L+  E  
Sbjct: 49  LPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAENIKALIEHEMK 108

Query: 156 ---PADIKLGVGGFSMGAATALYSA-TCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTL 215
              PA+ ++ +GGFS G A +LY+A TC          P+P  L+ +V LS WLP  +  
Sbjct: 109 NGIPAN-RIVLGGFSQGGALSLYTALTC----------PHP--LAGIVALSCWLPLHRNF 168

Query: 216 KSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSSEALRTCGF-QDVTFKSYSGLG 275
                G       A  L IL CHG++D +V   FG  ++E LRT      V FK+Y G+ 
Sbjct: 169 PQAANG------SAKDLAILQCHGELDPMVPVRFGALTAEKLRTVVTPARVQFKTYPGVM 224

Query: 276 HYTVPEEMDEVRAWL 284
           H + P+EM  V+ +L
Sbjct: 229 HSSCPQEMAAVKEFL 224

[Close]

BLAST of MS003767.1 vs. ExPASy Swiss-Prot
Match: Q9QYL8 (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-22
Identity = 70/195 (35.90%), Postives = 102/195 (52.31%), Query Frame = 0


Query: 96  LMQIKWICPTAPTQPIALFGGFPSTAWFDVGNLSEDGPDDLEGLDASAAHVSYLLSTE-- 155
           L  +K+ICP AP  P+ L       +WFD+  LS D P+D  G+  +A ++  L+  E  
Sbjct: 49  LPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAENIKALIEHEMK 108

Query: 156 ---PADIKLGVGGFSMGAATALYSA-TCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTL 215
              PA+ ++ +GGFS G A +LY+A TC          P+P  L+ +V LS WLP  +  
Sbjct: 109 NGIPAN-RIVLGGFSQGGALSLYTALTC----------PHP--LAGIVALSCWLPLHRNF 168

Query: 216 KSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSSEALRTCGF-QDVTFKSYSGLG 275
                G       A  L IL CHG++D +V   FG  ++E LRT      V FK+Y G+ 
Sbjct: 169 PQAANG------SAKDLAILQCHGELDPMVPVRFGALTAEKLRTVVTPARVQFKTYPGVM 224

Query: 276 HYTVPEEMDEVRAWL 284
           H + P+EM  V+ +L
Sbjct: 229 HSSCPQEMAAVKEFL 224

[Close]

BLAST of MS003767.1 vs. ExPASy Swiss-Prot
Match: O95372 (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 5.9e-22
Identity = 69/195 (35.38%), Postives = 102/195 (52.31%), Query Frame = 0


Query: 96  LMQIKWICPTAPTQPIALFGGFPSTAWFDVGNLSEDGPDDLEGLDASAAHVSYLLSTE-- 155
           L  +K+ICP AP  P+ L       +WFD+  LS D P+D  G+  +A ++  L+  E  
Sbjct: 49  LPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAENIKALIEHEMK 108

Query: 156 ---PADIKLGVGGFSMGAATALYSA-TCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTL 215
              PA+ ++ +GGFS G A +LY+A TC          P+P  L+ +V LS WLP  +  
Sbjct: 109 NGIPAN-RIVLGGFSQGGALSLYTALTC----------PHP--LAGIVALSCWLPLHRAF 168

Query: 216 KSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSSEALRTCGF-QDVTFKSYSGLG 275
                G       A  L IL CHG++D +V   FG  ++E LR+      V FK+Y G+ 
Sbjct: 169 PQAANG------SAKDLAILQCHGELDPMVPVRFGALTAEKLRSVVTPARVQFKTYPGVM 224

Query: 276 HYTVPEEMDEVRAWL 284
           H + P+EM  V+ +L
Sbjct: 229 HSSCPQEMAAVKEFL 224

[Close]

BLAST of MS003767.1 vs. ExPASy Swiss-Prot
Match: Q54T49 (Acyl-protein thioesterase 1 homolog 1 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0282005 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.2e-21
Identity = 71/260 (27.31%), Postives = 118/260 (45.38%), Query Frame = 0


Query: 23  TYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLIKWVKSRLKNSCWKR 82
           +Y+ +    H ATV++ HGLGD+G+ +                 +++ ++SR        
Sbjct: 9   SYIQKEITTHSATVIFSHGLGDSGAGWIE---------------VMEEIQSRNNG----- 68

Query: 83  KSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNLSEDGPDDLEGLDAS 142
                           I++ICP AP Q + L GGF   +W+D+ +LS  G +D   +D S
Sbjct: 69  ---------------HIRFICPNAPIQAVTLNGGFKMPSWYDIKSLSSRGDEDPAQVDES 128

Query: 143 AAHVSYLLSTEPADIKLG-----VGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVV 202
              +  ++  E  + K+      +GGFS GAA +LY+       K G            +
Sbjct: 129 KNIIETIIKHEMEEEKIPAERIIIGGFSQGAALSLYTFYSQTETKLG----------GCI 188

Query: 203 GLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSSEALRTCGFQ 262
            LSG+LP +    +    K +        P+L+ HG  D VV + +G+ S + L++ G  
Sbjct: 189 ALSGYLPLATKFVANSLNKEQ--------PLLMIHGDCDQVVRHQWGKLSFDHLKSQGIN 214

Query: 263 DVTFKSYSGLGHYTVPEEMD 278
              F +  GLGH++ PEE+D
Sbjct: 249 G-EFITLKGLGHHSSPEEID 214

[Close]

BLAST of MS003767.1 vs. ExPASy TrEMBL
Match: A0A6J1CSZ8 (acyl-protein thioesterase 2 OS=Momordica charantia OX=3673 GN=LOC111014480 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.3e-125
Identity = 232/281 (82.56%), Postives = 236/281 (83.99%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGS +  + +L + P         
Sbjct: 10  SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSSW--SQLLETLP--------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPTQPIALFGGFPSTAWFDVGNL
Sbjct: 70  ---------------------------LPNIKWICPTAPTQPIALFGGFPSTAWFDVGNL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YPANLSAVVGLSGWLPCSKTLKSKMEGKNES SGAGSLPILLCHGKVDDVVLYNFGEKSS
Sbjct: 190 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESTSGAGSLPILLCHGKVDDVVLYNFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL
Sbjct: 250 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 252

[Close]

BLAST of MS003767.1 vs. ExPASy TrEMBL
Match: A0A6J1JJZ2 (acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=4 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 4.4e-113
Identity = 210/281 (74.73%), Postives = 222/281 (79.00%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           S GKAA++AIEFGKTYVVRPKGKH AT+VWLHGLGDNGS  +S  +   P          
Sbjct: 10  SGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSS-WSQLLEMLP---------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+PIALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRPIALFGGFPSTAWFDVKDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHV+YLLSTEP+DIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVAYLLSTEPSDIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YP NLSAVVGLSGWLPCSKTLKSKME KN S SGAGSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPTNLSAVVGLSGWLPCSKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           +AL + GFQDV FKSY+GLGHYTVPEEMDEV AWL SKLGL
Sbjct: 250 QALHSVGFQDVIFKSYNGLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of MS003767.1 vs. ExPASy TrEMBL
Match: A0A0A0LU24 (Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G305760 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 7.5e-113
Identity = 211/281 (75.09%), Postives = 224/281 (79.72%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           + G+AAKKAIEFGKTYVVRPKGKH ATVVWLHGLGDNGS +  + +L + P         
Sbjct: 10  AGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSW--SQLLETLP--------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+PIALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHV+YLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YPANLSAVVGLSGWLPCSKTLK+ ME KN   SGAGSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           EALR+ GF+DVTFKSY+ LGHYTVPEEMDEV AWL SKLGL
Sbjct: 250 EALRSSGFKDVTFKSYNSLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of MS003767.1 vs. ExPASy TrEMBL
Match: A0A6J1H9A6 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461683 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 3.7e-112
Identity = 209/281 (74.38%), Postives = 221/281 (78.65%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           S GKAA++AIEFGKTYVVRPKGKH AT+VWLHGLGDNGS  +S  +   P          
Sbjct: 10  SGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSS-WSQLLEMLP---------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+ IALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRSIALFGGFPSTAWFDVKDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SEDGPDDLEGLDASAAHV+YLLSTEP+DIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SEDGPDDLEGLDASAAHVAYLLSTEPSDIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YP NLSAVVGLSGWLPCSKTLKSKME KN S SGAGSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPTNLSAVVGLSGWLPCSKTLKSKMEEKNASNSGAGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           +AL + GFQDV FKSY+GLGHYTVPEEMDEV AWL SKLGL
Sbjct: 250 QALHSVGFQDVIFKSYNGLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of MS003767.1 vs. ExPASy TrEMBL
Match: A0A1S3CMW5 (acyl-protein thioesterase 2 OS=Cucumis melo OX=3656 GN=LOC103502735 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.9e-111
Identity = 210/281 (74.73%), Postives = 223/281 (79.36%), Query Frame = 0


Query: 9   SAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFFLI 68
           + G+AAKKAIEFGKTYVVRPKGKH ATVVWLHGLGDNGS +  + +L + P         
Sbjct: 10  AGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSW--SQLLETLP--------- 69

Query: 69  KWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVGNL 128
                                      L  IKWICPTAPT+PIALFGGFPSTAWFDV +L
Sbjct: 70  ---------------------------LPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 129

Query: 129 SEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 188
           SE+GPDDLEGLDASAAHV+YLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP
Sbjct: 130 SENGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNP 189

Query: 189 YPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEKSS 248
           YPANLSAVVGLSGWLPCSKTLK+KME K  S  GAGSLPILLCHGKVDDVVLY FGEKSS
Sbjct: 190 YPANLSAVVGLSGWLPCSKTLKTKMEEKKASNIGAGSLPILLCHGKVDDVVLYKFGEKSS 249

Query: 249 EALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           EAL + GF+DVTFKSYS LGHYTVPEEMDEV AWL SKLGL
Sbjct: 250 EALLSSGFKDVTFKSYSSLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of MS003767.1 vs. TAIR 10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 9.1e-95
Identity = 168/283 (59.36%), Postives = 209/283 (73.85%), Query Frame = 0


Query: 7   SLSAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFF 66
           ++ +G+  ++A+EFGKT+VVRPKGKH AT+VWLHGLGDNGS +  + +L + P       
Sbjct: 7   AVGSGRNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSW--SQLLETLP------- 66

Query: 67  LIKWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVG 126
                                        L  IKWICPTAP+QPI+LFGGFPSTAWFDV 
Sbjct: 67  -----------------------------LPNIKWICPTAPSQPISLFGGFPSTAWFDVV 126

Query: 127 NLSEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNG 186
           +++EDGPDD+EGLD +AAHV+ LLS EPADIKLGVGGFSMGAAT+LYSATC A+GKYGNG
Sbjct: 127 DINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAATSLYSATCFALGKYGNG 186

Query: 187 NPYPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEK 246
           NPYP NLSA++GLSGWLPC+KTL  K+E + +  + A SLPI++CHGK DDVV + FGEK
Sbjct: 187 NPYPINLSAIIGLSGWLPCAKTLAGKLE-EEQIKNRAASLPIVVCHGKADDVVPFKFGEK 246

Query: 247 SSEALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           SS+AL + GF+ VTFK YS LGH+T+P+E+DE+ AWL S L L
Sbjct: 247 SSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

[Close]

BLAST of MS003767.1 vs. TAIR 10
Match: AT5G20060.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 9.1e-95
Identity = 168/283 (59.36%), Postives = 209/283 (73.85%), Query Frame = 0


Query: 7   SLSAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFF 66
           ++ +G+  ++A+EFGKT+VVRPKGKH AT+VWLHGLGDNGS +  + +L + P       
Sbjct: 7   AVGSGRNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSW--SQLLETLP------- 66

Query: 67  LIKWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVG 126
                                        L  IKWICPTAP+QPI+LFGGFPSTAWFDV 
Sbjct: 67  -----------------------------LPNIKWICPTAPSQPISLFGGFPSTAWFDVV 126

Query: 127 NLSEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNG 186
           +++EDGPDD+EGLD +AAHV+ LLS EPADIKLGVGGFSMGAAT+LYSATC A+GKYGNG
Sbjct: 127 DINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAATSLYSATCFALGKYGNG 186

Query: 187 NPYPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEK 246
           NPYP NLSA++GLSGWLPC+KTL  K+E + +  + A SLPI++CHGK DDVV + FGEK
Sbjct: 187 NPYPINLSAIIGLSGWLPCAKTLAGKLE-EEQIKNRAASLPIVVCHGKADDVVPFKFGEK 246

Query: 247 SSEALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           SS+AL + GF+ VTFK YS LGH+T+P+E+DE+ AWL S L L
Sbjct: 247 SSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

[Close]

BLAST of MS003767.1 vs. TAIR 10
Match: AT5G20060.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 9.1e-95
Identity = 168/283 (59.36%), Postives = 209/283 (73.85%), Query Frame = 0


Query: 7   SLSAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFF 66
           ++ +G+  ++A+EFGKT+VVRPKGKH AT+VWLHGLGDNGS +  + +L + P       
Sbjct: 7   AVGSGRNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSW--SQLLETLP------- 66

Query: 67  LIKWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVG 126
                                        L  IKWICPTAP+QPI+LFGGFPSTAWFDV 
Sbjct: 67  -----------------------------LPNIKWICPTAPSQPISLFGGFPSTAWFDVV 126

Query: 127 NLSEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNG 186
           +++EDGPDD+EGLD +AAHV+ LLS EPADIKLGVGGFSMGAAT+LYSATC A+GKYGNG
Sbjct: 127 DINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAATSLYSATCFALGKYGNG 186

Query: 187 NPYPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEK 246
           NPYP NLSA++GLSGWLPC+KTL  K+E + +  + A SLPI++CHGK DDVV + FGEK
Sbjct: 187 NPYPINLSAIIGLSGWLPCAKTLAGKLE-EEQIKNRAASLPIVVCHGKADDVVPFKFGEK 246

Query: 247 SSEALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           SS+AL + GF+ VTFK YS LGH+T+P+E+DE+ AWL S L L
Sbjct: 247 SSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

[Close]

BLAST of MS003767.1 vs. TAIR 10
Match: AT3G15650.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 324.7 bits (831), Expect = 7.5e-89
Identity = 158/283 (55.83%), Postives = 195/283 (68.90%), Query Frame = 0


Query: 7   SLSAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFF 66
           S+ +G  + +  EFG+TYVVRPKGKH AT+VWLHGLGDNGSR  +  ++ +  F    F 
Sbjct: 7   SMGSGSRSTRGYEFGRTYVVRPKGKHQATIVWLHGLGDNGSRILACSLITTSHFGSVSFC 66

Query: 67  LIKWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVG 126
               +   L                    L  IKWICPTAP++P++L GGFP TAWFDVG
Sbjct: 67  SSSQLLESLP-------------------LPNIKWICPTAPSRPVSLLGGFPCTAWFDVG 126

Query: 127 NLSEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNG 186
            +SED  DD+EGLDASAAH++ LLS EP D+K+G+GGFSMGAA ALYS TC+A+G+YG G
Sbjct: 127 EISEDLHDDIEGLDASAAHIANLLSAEPTDVKVGIGGFSMGAAIALYSTTCYALGRYGTG 186

Query: 187 NPYPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEK 246
           + Y  NL A VGLSGWLP  ++L+SK+E  NE A  A S+PILL HG  DDVV Y FGEK
Sbjct: 187 HAYTINLRATVGLSGWLPGWRSLRSKIESSNEVARRAASIPILLAHGTSDDVVPYRFGEK 246

Query: 247 SSEALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           S+ +L   GF+   FK Y GLGHYTVP+EMDEV  WL+S+LGL
Sbjct: 247 SAHSLAMAGFRQTMFKPYEGLGHYTVPKEMDEVVHWLVSRLGL 270

[Close]

BLAST of MS003767.1 vs. TAIR 10
Match: AT3G15650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 2.4e-87
Identity = 158/283 (55.83%), Postives = 192/283 (67.84%), Query Frame = 0


Query: 7   SLSAGKAAKKAIEFGKTYVVRPKGKHLATVVWLHGLGDNGSRYYSNFILFSPPFSVAFFF 66
           S+ +G  + +  EFG+TYVVRPKGKH AT+VWLHGLGDNGS   S+ +L S P       
Sbjct: 7   SMGSGSRSTRGYEFGRTYVVRPKGKHQATIVWLHGLGDNGSS--SSQLLESLP------- 66

Query: 67  LIKWVKSRLKNSCWKRKSFLLVFCINLELLMQIKWICPTAPTQPIALFGGFPSTAWFDVG 126
                                        L  IKWICPTAP++P++L GGFP TAWFDVG
Sbjct: 67  -----------------------------LPNIKWICPTAPSRPVSLLGGFPCTAWFDVG 126

Query: 127 NLSEDGPDDLEGLDASAAHVSYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNG 186
            +SED  DD+EGLDASAAH++ LLS EP D+K+G+GGFSMGAA ALYS TC+A+G+YG G
Sbjct: 127 EISEDLHDDIEGLDASAAHIANLLSAEPTDVKVGIGGFSMGAAIALYSTTCYALGRYGTG 186

Query: 187 NPYPANLSAVVGLSGWLPCSKTLKSKMEGKNESASGAGSLPILLCHGKVDDVVLYNFGEK 246
           + Y  NL A VGLSGWLP  ++L+SK+E  NE A  A S+PILL HG  DDVV Y FGEK
Sbjct: 187 HAYTINLRATVGLSGWLPGWRSLRSKIESSNEVARRAASIPILLAHGTSDDVVPYRFGEK 246

Query: 247 SSEALRTCGFQDVTFKSYSGLGHYTVPEEMDEVRAWLISKLGL 290
           S+ +L   GF+   FK Y GLGHYTVP+EMDEV  WL+S+LGL
Sbjct: 247 SAHSLAMAGFRQTMFKPYEGLGHYTVPKEMDEVVHWLVSRLGL 251

The following BLAST results are available for this feature:

BLAST of MS003767.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144920.1	2.7e-125	82.56	acyl-protein thioesterase 2 [Momordica charantia] >XP_022144921.1 acyl-protein t...	[more]
XP_038879397.1	4.8e-114	75.44	acyl-protein thioesterase 2 [Benincasa hispida]	[more]
XP_022987844.1	9.1e-113	74.73	acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022987845.1 acyl-protein...	[more]
XP_004150012.1	1.6e-112	75.09	acyl-protein thioesterase 2 [Cucumis sativus] >XP_031736139.1 acyl-protein thioe...	[more]
XP_023515795.1	2.7e-112	74.73	acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS003767.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4I8Q4	4.1e-23	29.21	Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 12365...	[more]
Q9WTL7	2.0e-22	35.90	Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1	[more]
Q9QYL8	2.0e-22	35.90	Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1	[more]
O95372	5.9e-22	35.38	Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1	[more]
Q54T49	2.2e-21	27.31	Acyl-protein thioesterase 1 homolog 1 OS=Dictyostelium discoideum OX=44689 GN=DD...	[more]

BLAST of MS003767.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSZ8	1.3e-125	82.56	acyl-protein thioesterase 2 OS=Momordica charantia OX=3673 GN=LOC111014480 PE=4 ...	[more]
A0A6J1JJZ2	4.4e-113	74.73	acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=...	[more]
A0A0A0LU24	7.5e-113	75.09	Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G3057...	[more]
A0A6J1H9A6	3.7e-112	74.38	acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461683 P...	[more]
A0A1S3CMW5	1.9e-111	74.73	acyl-protein thioesterase 2 OS=Cucumis melo OX=3656 GN=LOC103502735 PE=4 SV=1	[more]

BLAST of MS003767.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20060.2	9.1e-95	59.36	alpha/beta-Hydrolases superfamily protein	[more]
AT5G20060.1	9.1e-95	59.36	alpha/beta-Hydrolases superfamily protein	[more]
AT5G20060.3	9.1e-95	59.36	alpha/beta-Hydrolases superfamily protein	[more]
AT3G15650.2	7.5e-89	55.83	alpha/beta-Hydrolases superfamily protein	[more]
AT3G15650.1	2.4e-87	55.83	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003140	Phospholipase/carboxylesterase/thioesterase	PFAM	PF02230	Abhydrolase_2	coord: 96..283 e-value: 3.6E-26 score: 92.3
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 12..288 e-value: 4.4E-56 score: 192.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 97..287
None	No IPR available	PANTHER	PTHR46234:SF9	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 8..47
None	No IPR available	PANTHER	PTHR46234:SF9	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 99..289
None	No IPR available	PANTHER	PTHR46234	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 8..47 coord: 99..289

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS003767	MS003767	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS003767.1-cds	MS003767.1-cds-scaffold127:299629..299698	CDS
MS003767.1-cds	MS003767.1-cds-scaffold127:299797..299890	CDS
MS003767.1-cds	MS003767.1-cds-scaffold127:300212..300294	CDS
MS003767.1-cds	MS003767.1-cds-scaffold127:300387..300537	CDS
MS003767.1-cds	MS003767.1-cds-scaffold127:300760..300864	CDS
MS003767.1-cds	MS003767.1-cds-scaffold127:300988..301117	CDS
MS003767.1-cds	MS003767.1-cds-scaffold127:301119..301206	CDS
MS003767.1-cds	MS003767.1-cds-scaffold127:302550..302695	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS003767.1	MS003767.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity