MS003732.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS003732.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	scaffold127: 50663 .. 55177 (-)
Sequence length	1905
RNA-Seq Expression	MS003732.1
Synteny	MS003732.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTGCCCAAGTTAGATGGAATCACTAAGAAAAAGGAAATCATCTTCCTAGCTCCTACAGGGGAGGAGATTAATGGCAAAAGGCAGCTGCATCAGTACCTGAAATCACACCCTGGTAATCCAGCATCTTCTGAGTTTGATTGGGGTACTGGTGAGACACCTAGAAAATCAGCTAGGATTAGTGAGAGGGTGAAGGCAGCTCCCTTGCCTCGAATTGAATGGCCTAAGAAACGAACTCGAAAACTGGCTGTTTCGAAAAAAGATGATAAAGAAGCAGATGTTGTCCTAGATGGTTCTGAGGAGGCAAGAGCAGTTCATAAGCAAGATGCTGATAAATATGAGAGCGGTGTTACTGATGGGAAGGGAAATCAAGAAAATAGAACACTGCAAGATGAGGATGTGGAAACTGCCCCAGCCCCTGCCCCGGCCTTGGCCTCCTCTGAACCTGCTGAAGAGGGGCAACAACTTGATGGTGAGGAATGCGAGAATCCTGCTGAAATAGAGTGGGGAAGCGCAGAAGGGAACAAGGAAACTGAAGGTTCTGAAGATGCAACAAAGGGAAGTCGAAATTTAGATGGAACAAAAGGGGAAGAAAAGGTGCAACTTCAAGATGATGCAAAGGAAACCATTGAATCAGAGGACAAGGAATCCATTTTGGGGGAAAAAGAGTTTGAGAATGCAAAGCAAGCGACCAGTCCAGGCGATAAGGCAGCGATTTGCAAAGAAACAATGGTGTCGGTCGAAGTGCAAGTAAACGAACCAAACGAATTCAACCCGAGTTCTACAAAATCAGGAGAAATCAAGATGGAAGAAGCAGCAAAAGGGACCAAAGAGGAACATAATAAAGAGGTTGACAGTTGACAGAATTGAGTGACTTTACATTTCTAGTTCTAACTGTTTCAAATATGTAAGATGTCCTGTGTTCGAACGTCGTTTATTCGATTCGATTCATATGCCGAGACATTCATGTATTGATATTTTTAGATTAGTTATTTGTACATACCATCTTTGTATAAAAATATTACATAGCACTTAGCAGTACAGTGCAGCTAAGGTATAATGCAGTCTTGTGATGATTTATTGAAGTACTTAACTTGGGAAACCTTATAATTACTCTCTGCTTCAGAATCTTGAAGCCAGTCTCTTAAATCCTTCTAAAATTAGCTGACAGAAAGTATGAAACATCTGTAATGGAACTTGTTCTCTAGATTATGTTGTAAACTTGTAATCAAGTAGCAGTTCAGAGTTTCATTGAAGATTCTAAGAAAGAAAATAATTTAATTTGTTTAGATTAAGAACAATAAACATCTATTATTTTAAATCAGTTCTATAAAATTATACAAGATCTAATGTGTCAGGCATCTTAACTATTTTCATTGACTTCCTTTTTTAATTCAAAGTGGATTAAGTAAGATATAGTTTAAAATTCGTCACCACTAATAATTGATTTGTTAAATAAATACATAGATTAGATTAAAATCATATAGATATAATTTATCACACAGCTGTGATTAAGGACCATTTGATTTTAATTTCTTTTTATGGGTCAATAAGTACAAATTGGACAAATGTATATTTCTTTTCTTTAGAACCCAAAAATAAATTTGAATCTGTGACGGGAATGAGAACATGATCATATGCAGAGAAATTAATACCAAATTACAGAAACTTTTGTTATTTTTAGCGTACTTTTTGTTAAAATAATAATTTATAATAGCATAATATGAAAAATATTGGTAAAGATATGGATTTTAAATCTCGAAAAAAGATAATTACTATAAAATTGATTTAACTCATTCATGTTTCAAGTTGCTGGAATAGCTCAGTTGGTTAGAGCGTGTGGCTGTTAACCACAAGGTCGGAGGTTCAAGCCCTCCTTCTAGCGTTTTTTTTTCTTTTGTTTTTTTTTTTTTAAACTTTTTTACCAAATTTTCTCCAAAACCCAGGTTCAGAAAAGCCAGAATTGAGTACTTACAGTGAGATTATGATTATTCACTTCGATATTTTGAAAACCCACAGGAAAAGAAGATCATCGCCCCCAAGAAAAATTGAAAGGGGGCACTGCTCGAACGTCACCATCTCATCTCAGGTAAAAATGGATGCAATTGCTATTTTGAAATTCGTCCCAATTCGTCAGAAAAACGCATGGGTTTTACAGCACCCAAAATACACAGTCAAGTGCTCGTAATGTCAATTTCATTTTATTCAATGCTGACCAAAGGGGATGAAGTGTGATGTGATCAGTTATAACACCAAGAAGTTGTGGAAGAAAGAGAGGGGAGAGGTTCCATCAAAACCCAGAAACCAGAAACCAAGAACCCCATTTGACTCCCCGAGTTTCAATTTCAATCGAAGACAATCGAGATAGAGATTTCTGTATATACAGTAAGAGGGTAAGTGGAGGGGGTTCGGTTAAAAGCAAAAGGGGGAATCTTTTGGCTTATGGCATGCCAGAGGAAGAAGAAGCAGAGCGTTTCAGTTTTTGAGTTTGGGATTGATTTGGTACTTCCCACCAGACCAAATCGCGATCTTTCTTTGAAGAATTTTTCTCATTTCTTCTTCTTATCTTCGTGGAAATGATGATCACCACGCGCTTATGCTTCTTGCGGCGGTTGATTCCCTTATTGCTTGTCTTCTTCTTTACACTTGGTATTGAATTTTCTTTCCCTTCACTTTTCTGCCTCTTTGTCAAACTATCTTTTCAAGTTTTTACGGACTTTAAGGCCCTGATTTAAATACAAAAGTAGGATGCATCGGTTTGCTTATACAAGCTCAAACTTGTAAGGTTGTCTTTTATGGACCATTTTTACTTTTCTGTTCTGCAATTTGTTTCTTCAGCTTGTTTTAGATTGACAATAATTATGCCTGTGAAATTTAGGTAGTCAACTTTGCTTCTGTTTTTCTTTAAAGGCTACTCTACTAATTTTGAAGCTTATGAGCTAGAAAGAAAACGTTGTGAAGATCTTAGTAACCAATATGATCAGTCATGTCAAACAAAGATTTTGAAGGAAATTGATTATTACATCAAAGATGTTAGCATTTCATCAATGCTTTTTGAACTGATATTATTCATTAGAACCATCCTCGATTTGTCGGTATGATAGTGCTTTGGTATTTTAAGTTAAAAGTCTGTATCGTTTTGATGCTCAAATTTTTATCATAGATCATGTGATCTGCCGTGTGGGTAGCGTGAGGATCACTGCCAACTTCCTACAGTTCTCTAGGAGAGAATTGAAGTGAAATATACTTTTCCATGTTCTTTAGAATTTCCGTCTGAATATATTTCCTTTAGGCCAATTTCATATTGTTATGATTTATGCATTTTCTGAACCTTTTCTGGCTCAAAATTGGTACTCATTAGTCATTAACTCGACTTTACGCACTCCTGTTAGCTGCACAAGATCTTTGTGGCTTTAAAATTTCATTTCGAATTCGTGCTTTCATCTTGACCTATCCTAGAACAGAGTGATTAATGGAATTACCTCCACCTTTTCTGCAGCTTCATTAACGGTGGAAGCATTTACTGGAACCTATGGAATAAACTATGGCCGAATCGCAAATAACATCCCTTCACCTGATAAAGTTGTTACTCTTCTTAGAGCTGCTAAGATAAAGAATGTTAGGATTTATGATGCTGATCACAGTGTTCTCAAGGCCTTCAGTGGGACAGGGCTCGAGATTGTCATTAGTATACCAAACGAGTACCTGAAAGATATGAGTGCAAATGAAGATCGTGCCTTGAACTGGGTTAAACAAAATGTGCAGCCCTTCCTTCCGGAGACACATATTTGTGGAATTGCTATAGGTAATGAAGTTTTGGGAGGGAGTGATTATGGATTATGGTCAGCATTGTTGGGTGCAGCCAAAAATGTTTATAATGCTGTAAAGAAGCTCAATTTAGATGGTATAATTCAGATTACTACAGCACATTCCCAAGCTGTCTTTTCTAATTCGTTTCCTCCCTCATCGTGTAAATTCGGAGATAATGTTGCTCAGTATATGAAACCTCTATTGGAGTTCTTCTCACAGATTGGCTCTCCTTTCTGTTTAAATGCATACCCATTTCTGGCCTACATGAGTGACCCAGGAAATATTGATATTAATTATGCTCTTTTTCTATCAACAGAAGGGATTTATGATCCTAAAACTGACCTACATTATGATAACATGCTCGATGCTCAGATCGATGCAGCTTATGCCGCACTGGAAGATGCTGGATATAAAGATATGGAAGTCATAGTTACAGAAACAGGATGGGCTTCACGTGGAGATGACAATGAAAAAGCAGCCACAGTAAATAATGCACAGACTTATAATTATAACTTGCGGAAACGTCTTGCCAAGAAGAAAGGAACTCCTCTTAGGCCGAAAAATGTGTTGAAGGCATATATTTTTGCATTATTTAATGAGAATTTGAAGTGGGGACCTACGTCTGAAAGAAACTATGGACTTTTCAAGCCTGATGGGAGCATTTCATATGATATTGGCTTTCAAGGACTTCAATCTTCATCTGCA

mRNA sequence

TTTTTGCCCAAGTTAGATGGAATCACTAAGAAAAAGGAAATCATCTTCCTAGCTCCTACAGGGGAGGAGATTAATGGCAAAAGGCAGCTGCATCAGTACCTGAAATCACACCCTGGTAATCCAGCATCTTCTGAGTTTGATTGGGGTACTGGTGAGACACCTAGAAAATCAGCTAGGATTAGTGAGAGGGTGAAGGCAGCTCCCTTGCCTCGAATTGAATGGCCTAAGAAACGAACTCGAAAACTGGCTGTTTCGAAAAAAGATGATAAAGAAGCAGATGTTGTCCTAGATGGTTCTGAGGAGGCAAGAGCAGTTCATAAGCAAGATGCTGATAAATATGAGAGCGGTGTTACTGATGGGAAGGGAAATCAAGAAAATAGAACACTGCAAGATGAGGATGTGGAAACTGCCCCAGCCCCTGCCCCGGCCTTGGCCTCCTCTGAACCTGCTGAAGAGGGGCAACAACTTGATGGTGAGGAATGCGAGAATCCTGCTGAAATAGAGTGGGGAAGCGCAGAAGGGAACAAGGAAACTGAAGGTTCTGAAGATGCAACAAAGGGAAGTCGAAATTTAGATGGAACAAAAGGGGAAGAAAAGGTGCAACTTCAAGATGATGCAAAGGAAACCATTGAATCAGAGGACAAGGAATCCATTTTGGGGGAAAAAGAGTTTGAGAATGCAAAGCAAGCGACCAGTCCAGGCGATAAGGCAGCGATTTGCAAAGAAACAATGGTGTCGGTCGAAGTGCAAGTAAACGAACCAAACGAATTCAACCCGAGTTCTACAAAATCAGGAGAAATCAAGATGGAAGAAGCAGCAAAAGGGACCAAAGAGGAACATAATAAAGAGGTTGACATGATTAATGGAATTACCTCCACCTTTTCTGCAGCTTCATTAACGGTGGAAGCATTTACTGGAACCTATGGAATAAACTATGGCCGAATCGCAAATAACATCCCTTCACCTGATAAAGTTGTTACTCTTCTTAGAGCTGCTAAGATAAAGAATGTTAGGATTTATGATGCTGATCACAGTGTTCTCAAGGCCTTCAGTGGGACAGGGCTCGAGATTGTCATTAGTATACCAAACGAGTACCTGAAAGATATGAGTGCAAATGAAGATCGTGCCTTGAACTGGGTTAAACAAAATGTGCAGCCCTTCCTTCCGGAGACACATATTTGTGGAATTGCTATAGGTAATGAAGTTTTGGGAGGGAGTGATTATGGATTATGGTCAGCATTGTTGGGTGCAGCCAAAAATGTTTATAATGCTGTAAAGAAGCTCAATTTAGATGGTATAATTCAGATTACTACAGCACATTCCCAAGCTGTCTTTTCTAATTCGTTTCCTCCCTCATCGTGTAAATTCGGAGATAATGTTGCTCAGTATATGAAACCTCTATTGGAGTTCTTCTCACAGATTGGCTCTCCTTTCTGTTTAAATGCATACCCATTTCTGGCCTACATGAGTGACCCAGGAAATATTGATATTAATTATGCTCTTTTTCTATCAACAGAAGGGATTTATGATCCTAAAACTGACCTACATTATGATAACATGCTCGATGCTCAGATCGATGCAGCTTATGCCGCACTGGAAGATGCTGGATATAAAGATATGGAAGTCATAGTTACAGAAACAGGATGGGCTTCACGTGGAGATGACAATGAAAAAGCAGCCACAGTAAATAATGCACAGACTTATAATTATAACTTGCGGAAACGTCTTGCCAAGAAGAAAGGAACTCCTCTTAGGCCGAAAAATGTGTTGAAGGCATATATTTTTGCATTATTTAATGAGAATTTGAAGTGGGGACCTACGTCTGAAAGAAACTATGGACTTTTCAAGCCTGATGGGAGCATTTCATATGATATTGGCTTTCAAGGACTTCAATCTTCATCTGCA

Coding sequence (CDS)

TTTTTGCCCAAGTTAGATGGAATCACTAAGAAAAAGGAAATCATCTTCCTAGCTCCTACAGGGGAGGAGATTAATGGCAAAAGGCAGCTGCATCAGTACCTGAAATCACACCCTGGTAATCCAGCATCTTCTGAGTTTGATTGGGGTACTGGTGAGACACCTAGAAAATCAGCTAGGATTAGTGAGAGGGTGAAGGCAGCTCCCTTGCCTCGAATTGAATGGCCTAAGAAACGAACTCGAAAACTGGCTGTTTCGAAAAAAGATGATAAAGAAGCAGATGTTGTCCTAGATGGTTCTGAGGAGGCAAGAGCAGTTCATAAGCAAGATGCTGATAAATATGAGAGCGGTGTTACTGATGGGAAGGGAAATCAAGAAAATAGAACACTGCAAGATGAGGATGTGGAAACTGCCCCAGCCCCTGCCCCGGCCTTGGCCTCCTCTGAACCTGCTGAAGAGGGGCAACAACTTGATGGTGAGGAATGCGAGAATCCTGCTGAAATAGAGTGGGGAAGCGCAGAAGGGAACAAGGAAACTGAAGGTTCTGAAGATGCAACAAAGGGAAGTCGAAATTTAGATGGAACAAAAGGGGAAGAAAAGGTGCAACTTCAAGATGATGCAAAGGAAACCATTGAATCAGAGGACAAGGAATCCATTTTGGGGGAAAAAGAGTTTGAGAATGCAAAGCAAGCGACCAGTCCAGGCGATAAGGCAGCGATTTGCAAAGAAACAATGGTGTCGGTCGAAGTGCAAGTAAACGAACCAAACGAATTCAACCCGAGTTCTACAAAATCAGGAGAAATCAAGATGGAAGAAGCAGCAAAAGGGACCAAAGAGGAACATAATAAAGAGGTTGACATGATTAATGGAATTACCTCCACCTTTTCTGCAGCTTCATTAACGGTGGAAGCATTTACTGGAACCTATGGAATAAACTATGGCCGAATCGCAAATAACATCCCTTCACCTGATAAAGTTGTTACTCTTCTTAGAGCTGCTAAGATAAAGAATGTTAGGATTTATGATGCTGATCACAGTGTTCTCAAGGCCTTCAGTGGGACAGGGCTCGAGATTGTCATTAGTATACCAAACGAGTACCTGAAAGATATGAGTGCAAATGAAGATCGTGCCTTGAACTGGGTTAAACAAAATGTGCAGCCCTTCCTTCCGGAGACACATATTTGTGGAATTGCTATAGGTAATGAAGTTTTGGGAGGGAGTGATTATGGATTATGGTCAGCATTGTTGGGTGCAGCCAAAAATGTTTATAATGCTGTAAAGAAGCTCAATTTAGATGGTATAATTCAGATTACTACAGCACATTCCCAAGCTGTCTTTTCTAATTCGTTTCCTCCCTCATCGTGTAAATTCGGAGATAATGTTGCTCAGTATATGAAACCTCTATTGGAGTTCTTCTCACAGATTGGCTCTCCTTTCTGTTTAAATGCATACCCATTTCTGGCCTACATGAGTGACCCAGGAAATATTGATATTAATTATGCTCTTTTTCTATCAACAGAAGGGATTTATGATCCTAAAACTGACCTACATTATGATAACATGCTCGATGCTCAGATCGATGCAGCTTATGCCGCACTGGAAGATGCTGGATATAAAGATATGGAAGTCATAGTTACAGAAACAGGATGGGCTTCACGTGGAGATGACAATGAAAAAGCAGCCACAGTAAATAATGCACAGACTTATAATTATAACTTGCGGAAACGTCTTGCCAAGAAGAAAGGAACTCCTCTTAGGCCGAAAAATGTGTTGAAGGCATATATTTTTGCATTATTTAATGAGAATTTGAAGTGGGGACCTACGTCTGAAAGAAACTATGGACTTTTCAAGCCTGATGGGAGCATTTCATATGATATTGGCTTTCAAGGACTTCAATCTTCATCTGCA

Protein sequence

FLPKLDGITKKKEIIFLAPTGEEINGKRQLHQYLKSHPGNPASSEFDWGTGETPRKSARISERVKAAPLPRIEWPKKRTRKLAVSKKDDKEADVVLDGSEEARAVHKQDADKYESGVTDGKGNQENRTLQDEDVETAPAPAPALASSEPAEEGQQLDGEECENPAEIEWGSAEGNKETEGSEDATKGSRNLDGTKGEEKVQLQDDAKETIESEDKESILGEKEFENAKQATSPGDKAAICKETMVSVEVQVNEPNEFNPSSTKSGEIKMEEAAKGTKEEHNKEVDMINGITSTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA

Homology

[Close]

BLAST of MS003732.1 vs. NCBI nr
Match: XP_022144806.1 (glucan endo-1,3-beta-glucosidase 14-like isoform X1 [Momordica charantia])

HSP 1 Score: 691.4 bits (1783), Expect = 7.2e-195
Identity = 340/342 (99.42%), Postives = 341/342 (99.71%), Query Frame = 0


Query: 294 FSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGT 353
           F+ ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGT
Sbjct: 22  FTLASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGT 81

Query: 354 GLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSA 413
           GLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSA
Sbjct: 82  GLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSA 141

Query: 414 LLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQ 473
           LLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQ
Sbjct: 142 LLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQ 201

Query: 474 IGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALE 533
           IGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALE
Sbjct: 202 IGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALE 261

Query: 534 DAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAY 593
           DAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAY
Sbjct: 262 DAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAY 321

Query: 594 IFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           IFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 322 IFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 363

[Close]

BLAST of MS003732.1 vs. NCBI nr
Match: KAA0050153.1 (glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo var. makuwa] >TYK06409.1 glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo var. makuwa])

HSP 1 Score: 658.7 bits (1698), Expect = 5.2e-185
Identity = 323/346 (93.35%), Postives = 336/346 (97.11%), Query Frame = 0


Query: 290 ITSTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA 349
           I STF+AA LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA
Sbjct: 3   IISTFTAALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA 62

Query: 350 FSGTGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYG 409
           FSGTGLEIVISIPNE LKDMSANED A+NWVKQNVQPFLP+T ICGIAIGNEVLGGSD+ 
Sbjct: 63  FSGTGLEIVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSDFE 122

Query: 410 LWSALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLE 469
           LW+ LLGAAKNVYNAVKKL+LDG+IQITTAHSQAVFSNSFPPSSCKF DNVAQYMKPLL+
Sbjct: 123 LWTVLLGAAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQ 182

Query: 470 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAY 529
           FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLST+GIYDPKT LHYDNMLDAQIDAAY
Sbjct: 183 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAY 242

Query: 530 AALEDAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNV 589
           AALEDAGYKDMEVIVTETGWAS GDDNEKAAT++NA+TYNYNLRKRLAKKKGTPLRPKNV
Sbjct: 243 AALEDAGYKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNV 302

Query: 590 LKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           +KAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 303 VKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 348

[Close]

BLAST of MS003732.1 vs. NCBI nr
Match: KAE8637190.1 (hypothetical protein CSA_021703 [Cucumis sativus])

HSP 1 Score: 656.8 bits (1693), Expect = 2.0e-184
Identity = 321/346 (92.77%), Postives = 336/346 (97.11%), Query Frame = 0


Query: 290 ITSTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA 349
           I STF+AA LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA
Sbjct: 3   IISTFTAALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA 62

Query: 350 FSGTGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYG 409
           FSGTGLEIVISIPNE LKDMSANED A+NWVKQNVQPFLP+T ICGIAIGNE+LGGSD+ 
Sbjct: 63  FSGTGLEIVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEILGGSDFE 122

Query: 410 LWSALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLE 469
           LW+ LLGAAKNVYNAVKKL+LDG+IQITTAHSQAVFSNSFPPSSCKF DNVAQYMKPLL+
Sbjct: 123 LWTVLLGAAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQ 182

Query: 470 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAY 529
           FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLST+GIYDPKT LHYDNMLDAQIDAAY
Sbjct: 183 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAY 242

Query: 530 AALEDAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNV 589
           AALEDAGYKDMEVIVTETGWAS GD+NEKAAT++NA+TYNYNLRKRLAKKKGTPLRPKNV
Sbjct: 243 AALEDAGYKDMEVIVTETGWASHGDENEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNV 302

Query: 590 LKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           +KAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 303 VKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 348

[Close]

BLAST of MS003732.1 vs. NCBI nr
Match: XP_008443979.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo])

HSP 1 Score: 650.2 bits (1676), Expect = 1.8e-182
Identity = 318/339 (93.81%), Postives = 330/339 (97.35%), Query Frame = 0


Query: 297 ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 356
           A LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE
Sbjct: 23  ALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 82

Query: 357 IVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLG 416
           IVISIPNE LKDMSANED A+NWVKQNVQPFLP+T ICGIAIGNEVLGGSD+ LW+ LLG
Sbjct: 83  IVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSDFELWTVLLG 142

Query: 417 AAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGS 476
           AAKNVYNAVKKL+LDG+IQITTAHSQAVFSNSFPPSSCKF DNVAQYMKPLL+FFSQIGS
Sbjct: 143 AAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 202

Query: 477 PFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAG 536
           PFCLNAYPFLAYMSDPGNIDINYALFLST+GIYDPKT LHYDNMLDAQIDAAYAALEDAG
Sbjct: 203 PFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 262

Query: 537 YKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFA 596
           YKDMEVIVTETGWAS GDDNEKAAT++NA+TYNYNLRKRLAKKKGTPLRPKNV+KAYIFA
Sbjct: 263 YKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 322

Query: 597 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 323 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 361

[Close]

BLAST of MS003732.1 vs. NCBI nr
Match: XP_031745641.1 (glucan endo-1,3-beta-glucosidase 14-like [Cucumis sativus])

HSP 1 Score: 648.3 bits (1671), Expect = 7.0e-182
Identity = 316/339 (93.22%), Postives = 330/339 (97.35%), Query Frame = 0


Query: 297 ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 356
           A LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE
Sbjct: 22  ALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 81

Query: 357 IVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLG 416
           IVISIPNE LKDMSANED A+NWVKQNVQPFLP+T ICGIAIGNE+LGGSD+ LW+ LLG
Sbjct: 82  IVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEILGGSDFELWTVLLG 141

Query: 417 AAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGS 476
           AAKNVYNAVKKL+LDG+IQITTAHSQAVFSNSFPPSSCKF DNVAQYMKPLL+FFSQIGS
Sbjct: 142 AAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 201

Query: 477 PFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAG 536
           PFCLNAYPFLAYMSDPGNIDINYALFLST+GIYDPKT LHYDNMLDAQIDAAYAALEDAG
Sbjct: 202 PFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 261

Query: 537 YKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFA 596
           YKDMEVIVTETGWAS GD+NEKAAT++NA+TYNYNLRKRLAKKKGTPLRPKNV+KAYIFA
Sbjct: 262 YKDMEVIVTETGWASHGDENEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 321

Query: 597 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 322 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 360

[Close]

BLAST of MS003732.1 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 334.0 bits (855), Expect = 3.8e-90
Identity = 157/321 (48.91%), Postives = 223/321 (69.47%), Query Frame = 0


Query: 309 GINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIPNEYLKD 368
           GINYG+IANN+PSP +V  LLR+  I  V++YDAD +VL +FS + ++ +I + NEYL++
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 369 MSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYNAVKKL 428
           MS +  +A +W++Q ++P + +T I  I +GNE+   +D+ L  +LL A K+VY A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 429 NLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAYPFLAY 488
            L+  + +T+AHS  + S S+PPSS  F +   QY++PLL+F SQI SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 489 MSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVIVTETG 548
              P  + + Y LF   +G+ DP T+LHYDNML AQ+DA Y+A++  G+ D+EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 549 WASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENLKWGPTS 608
           W S+GD+NE  A+  NA  YN NL K + ++KGTP +    +  Y+FALFNENLK GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 609 ERNYGLFKPDGSISYDIGFQG 630
           ERNYGLF PDG   Y++G QG
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

[Close]

BLAST of MS003732.1 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.1e-89
Identity = 163/325 (50.15%), Postives = 222/325 (68.31%), Query Frame = 0


Query: 305 TGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIPNE 364
           T + G+NYG+I +N+PSP  V+ L+++     V++YDA+  +LKAFS TG+E +I + NE
Sbjct: 26  TTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSNTGIEFIIGLGNE 85

Query: 365 YLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYNA 424
           YL  M  +  +AL W+KQNV PFLP T+I  I IGNE+L  +D  L + LL A + V++A
Sbjct: 86  YLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTTNLLPAMQGVHSA 145

Query: 425 VKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAYP 484
           +    L   I +TTAHS ++  +SFPPS+ +F  ++   + P+LEF  +  SPF +NAYP
Sbjct: 146 LITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHRKTDSPFLINAYP 205

Query: 485 FLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVIV 544
           F AY  +P  + +++ LF   +GI DP T  HYDNML AQIDA Y+AL  AG+K + V +
Sbjct: 206 FFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALAAAGFKSLRVEI 265

Query: 545 TETGWASRGDDNEKAATVNNAQTYNYNLRKRL--AKKKGTPLRPKNVLKAYIFALFNENL 604
           +ETGW S+GDD+E  AT  NA+ YN NL K +   KK  TPL+P N L  Y+FALFNENL
Sbjct: 266 SETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDLSIYVFALFNENL 325

Query: 605 KWGPTSERNYGLFKPDGSISYDIGF 628
           K GPTSERNYGLFKPDG+ +Y +GF
Sbjct: 326 KPGPTSERNYGLFKPDGTQAYSLGF 349

[Close]

BLAST of MS003732.1 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.9e-82
Identity = 153/352 (43.47%), Postives = 226/352 (64.20%), Query Frame = 0


Query: 287 INGITSTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSV 346
           +  + S F  A  ++     + GINYG++ANN+P P  V+ LL++     V++YDAD   
Sbjct: 6   LQSLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQA 65

Query: 347 LKAFSGTGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGS 406
           L+AF+G+G E+ +++ NEYL  MS +  +A  WVK+NVQ +LP T I  I +GNEVL  +
Sbjct: 66  LRAFAGSGFELTVALGNEYLAQMS-DPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSN 125

Query: 407 DYGLWSALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKP 466
              L +AL  A ++++ A+    L+  I +TTAHS A+   S+PPS+  F  ++   + P
Sbjct: 126 QSALTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTP 185

Query: 467 LLEFFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQID 526
           +L+F  + GSP  +NAYPF AY  +P ++ +++ LF   +G  DP ++ HYDNML AQ+D
Sbjct: 186 ILDFHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVD 245

Query: 527 AAYAALEDAG--YKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRK-RLAKKKGTP 586
           A Y AL+  G  YK + ++V+ETGW S GD  E  AT +NA+ YN NL K  ++KK  TP
Sbjct: 246 AVYHALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTP 305

Query: 587 LRPKNVLKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           +RP+  L  ++FALFNEN+K GPTSERNYGLF PDG+  Y +G +   + S+
Sbjct: 306 IRPECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSS 356

[Close]

BLAST of MS003732.1 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 293.5 bits (750), Expect = 5.7e-78
Identity = 146/326 (44.79%), Postives = 209/326 (64.11%), Query Frame = 0


Query: 309 GINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIPNEYLKD 368
           G+NYG++A+N+P P + V LL++  I+ VR+Y AD +++KA +GTG+ IVI   N  +  
Sbjct: 27  GVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPS 86

Query: 369 MSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYNAVKKL 428
           ++++ + A  W+  NV PF P + I  I +GNE+L  +D  L + LL A +NV  A++ +
Sbjct: 87  LASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEAV 146

Query: 429 NLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAYPFLAY 488
           +L G I+++T +S  V  +S PPSS  F       +K +L+F S  GSPF +N YPF AY
Sbjct: 147 SLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAY 206

Query: 489 MSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVIVTETG 548
            SDP    + + LF    G  D KT + Y NM DAQ+DA ++AL+  G++ +E++V ETG
Sbjct: 207 QSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETG 266

Query: 549 WASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENLKWGPTS 608
           WASRGD NE  A+V+NA+ YN NL   L    GTPL P   +  YIFAL++ENLK GP+S
Sbjct: 267 WASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPSS 326

Query: 609 ERNYGLFKPDGSISYDIGFQGLQSSS 635
           ER +GLFK D S+ YD+G     SSS
Sbjct: 327 ERAFGLFKTDLSMVYDVGLAKSSSSS 352

[Close]

BLAST of MS003732.1 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 6.1e-72
Identity = 134/327 (40.98%), Postives = 201/327 (61.47%), Query Frame = 0


Query: 304 FTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIPN 363
           + G  G+ YGR A+++P+P KVV L++   IK VRIYD +  VLKAF  T +E++I +PN
Sbjct: 22  YGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPN 81

Query: 364 EYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYN 423
             L   S ++     W+K +V P+ P T I  I +G E          S ++ A +NV  
Sbjct: 82  SDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLT 141

Query: 424 AVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAY 483
           A++K+ L   I+++T  S  + S SFPPS+  F  + A +++P+LEF ++  SPF ++ Y
Sbjct: 142 ALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLY 201

Query: 484 PFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVI 543
           P+ AY   P N+ ++Y LF S+  + DP T L Y NM DAQ+DA Y AL    ++ ++++
Sbjct: 202 PYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIM 261

Query: 544 VTETGWASRGDDNEK-AATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENL 603
           VTETGW ++G   EK AA+ +NA+TYN N+ + +   +GTP +P   +  YIF+LFNEN 
Sbjct: 262 VTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENR 321

Query: 604 KWGPTSERNYGLFKPDGSISYDIGFQG 630
           K G  SERN+GLF PD +  Y + F G
Sbjct: 322 KAGLDSERNWGLFYPDQTSVYQLDFTG 348

[Close]

BLAST of MS003732.1 vs. ExPASy TrEMBL
Match: A0A6J1CUI0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014401 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 3.5e-195
Identity = 340/342 (99.42%), Postives = 341/342 (99.71%), Query Frame = 0


Query: 294 FSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGT 353
           F+ ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGT
Sbjct: 22  FTLASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGT 81

Query: 354 GLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSA 413
           GLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSA
Sbjct: 82  GLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSA 141

Query: 414 LLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQ 473
           LLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQ
Sbjct: 142 LLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQ 201

Query: 474 IGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALE 533
           IGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALE
Sbjct: 202 IGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALE 261

Query: 534 DAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAY 593
           DAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAY
Sbjct: 262 DAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAY 321

Query: 594 IFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           IFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 322 IFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 363

[Close]

BLAST of MS003732.1 vs. ExPASy TrEMBL
Match: A0A5A7U2L6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001100 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 2.5e-185
Identity = 323/346 (93.35%), Postives = 336/346 (97.11%), Query Frame = 0


Query: 290 ITSTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA 349
           I STF+AA LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA
Sbjct: 3   IISTFTAALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA 62

Query: 350 FSGTGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYG 409
           FSGTGLEIVISIPNE LKDMSANED A+NWVKQNVQPFLP+T ICGIAIGNEVLGGSD+ 
Sbjct: 63  FSGTGLEIVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSDFE 122

Query: 410 LWSALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLE 469
           LW+ LLGAAKNVYNAVKKL+LDG+IQITTAHSQAVFSNSFPPSSCKF DNVAQYMKPLL+
Sbjct: 123 LWTVLLGAAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQ 182

Query: 470 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAY 529
           FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLST+GIYDPKT LHYDNMLDAQIDAAY
Sbjct: 183 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAY 242

Query: 530 AALEDAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNV 589
           AALEDAGYKDMEVIVTETGWAS GDDNEKAAT++NA+TYNYNLRKRLAKKKGTPLRPKNV
Sbjct: 243 AALEDAGYKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNV 302

Query: 590 LKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           +KAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 303 VKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 348

[Close]

BLAST of MS003732.1 vs. ExPASy TrEMBL
Match: A0A1S3B8U7 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487436 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 8.9e-183
Identity = 318/339 (93.81%), Postives = 330/339 (97.35%), Query Frame = 0


Query: 297 ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 356
           A LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE
Sbjct: 23  ALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 82

Query: 357 IVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLG 416
           IVISIPNE LKDMSANED A+NWVKQNVQPFLP+T ICGIAIGNEVLGGSD+ LW+ LLG
Sbjct: 83  IVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSDFELWTVLLG 142

Query: 417 AAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGS 476
           AAKNVYNAVKKL+LDG+IQITTAHSQAVFSNSFPPSSCKF DNVAQYMKPLL+FFSQIGS
Sbjct: 143 AAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 202

Query: 477 PFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAG 536
           PFCLNAYPFLAYMSDPGNIDINYALFLST+GIYDPKT LHYDNMLDAQIDAAYAALEDAG
Sbjct: 203 PFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 262

Query: 537 YKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFA 596
           YKDMEVIVTETGWAS GDDNEKAAT++NA+TYNYNLRKRLAKKKGTPLRPKNV+KAYIFA
Sbjct: 263 YKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 322

Query: 597 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA
Sbjct: 323 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 361

[Close]

BLAST of MS003732.1 vs. ExPASy TrEMBL
Match: A0A6J1JHR0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485203 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 8.6e-178
Identity = 313/343 (91.25%), Postives = 328/343 (95.63%), Query Frame = 0


Query: 293 TFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSG 352
           +F  ASLT+EAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSG
Sbjct: 21  SFPPASLTIEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSG 80

Query: 353 TGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWS 412
           TGLE+VISIPNEYLKDMSANED ALNWVKQNVQPFLPET I GIAIGNEVLGGSD+ LW+
Sbjct: 81  TGLELVISIPNEYLKDMSANEDHALNWVKQNVQPFLPETLIRGIAIGNEVLGGSDFELWT 140

Query: 413 ALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFS 472
            LLGAAKNV+NA+KKL LD  IQITTAHSQAVFSNSFPPSSCKF +NVAQYMKPLL+FFS
Sbjct: 141 VLLGAAKNVHNALKKLGLDDGIQITTAHSQAVFSNSFPPSSCKFRENVAQYMKPLLQFFS 200

Query: 473 QIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAAL 532
           QIGSPFCLNAYPFLAYMSDP NIDINYALFLST+GI DPKT LHYDNMLDAQIDAAYAAL
Sbjct: 201 QIGSPFCLNAYPFLAYMSDPENIDINYALFLSTKGIDDPKTRLHYDNMLDAQIDAAYAAL 260

Query: 533 EDAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKA 592
           EDAGYKDMEVIVTETGWASRGDDNE+AATV NA+TYNYNLRKRLAKKKGTPLRPK+V+KA
Sbjct: 261 EDAGYKDMEVIVTETGWASRGDDNEEAATVGNARTYNYNLRKRLAKKKGTPLRPKHVVKA 320

Query: 593 YIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           YIFALFNENLKWGPTSERNYGLFKPDGSI+YDIGFQGLQSSSA
Sbjct: 321 YIFALFNENLKWGPTSERNYGLFKPDGSIAYDIGFQGLQSSSA 363

[Close]

BLAST of MS003732.1 vs. ExPASy TrEMBL
Match: A0A6J1HB81 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461741 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 8.6e-178
Identity = 313/344 (90.99%), Postives = 329/344 (95.64%), Query Frame = 0


Query: 292 STFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFS 351
           S+F  ASLT+EAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFS
Sbjct: 20  SSFPPASLTIEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFS 79

Query: 352 GTGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLW 411
           GTGLE+VISIPNEYLKDMSANED ALNWVKQNVQPFLPET I GIAIGNEVLGGSD+ LW
Sbjct: 80  GTGLELVISIPNEYLKDMSANEDHALNWVKQNVQPFLPETLIRGIAIGNEVLGGSDFELW 139

Query: 412 SALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFF 471
           + LLGAAKNV+NA+KKL LD  IQITTAHSQAVFSNSFPPSSCKF +NVAQYMKPLL+FF
Sbjct: 140 TVLLGAAKNVHNALKKLGLDDGIQITTAHSQAVFSNSFPPSSCKFRENVAQYMKPLLQFF 199

Query: 472 SQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAA 531
           SQIGSPFCLNAYPFLAYMSDP NIDINYALFLST+GI DPKT LHYDNMLDAQIDAAYAA
Sbjct: 200 SQIGSPFCLNAYPFLAYMSDPENIDINYALFLSTKGIDDPKTRLHYDNMLDAQIDAAYAA 259

Query: 532 LEDAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLK 591
           LEDAGYKDMEVIVTETGWASRGDDNE+AATV NA+TYN+NLRKRLAKKKGTPLRPK+V+K
Sbjct: 260 LEDAGYKDMEVIVTETGWASRGDDNEEAATVGNARTYNFNLRKRLAKKKGTPLRPKHVVK 319

Query: 592 AYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           AYIFALFNENLKWGPTSERNYGLFKPDGSI+YDIGFQGLQSSSA
Sbjct: 320 AYIFALFNENLKWGPTSERNYGLFKPDGSIAYDIGFQGLQSSSA 363

[Close]

BLAST of MS003732.1 vs. TAIR 10
Match: AT2G26600.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 521.5 bits (1342), Expect = 9.2e-148
Identity = 251/332 (75.60%), Postives = 289/332 (87.05%), Query Frame = 0


Query: 303 AFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIP 362
           AF GTYGINYGRIA+NIPSP+KVV LL+ AKI+NVRIYD DH+VL+AFSGTGL++V+ +P
Sbjct: 29  AFVGTYGINYGRIADNIPSPEKVVLLLKQAKIRNVRIYDVDHTVLEAFSGTGLDLVVGLP 88

Query: 363 NEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVY 422
           N +LK+MS+N D A +WVK+N+Q FLP+T I GIAIGNEVLGG D  L  ALLGAAKNVY
Sbjct: 89  NGFLKEMSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVY 148

Query: 423 NAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNA 482
           NA+KK+NL+  +QITTAHSQAVFS+S+PPSSC F +NV Q+MKPLLEFF QIGSPFCLNA
Sbjct: 149 NALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNA 208

Query: 483 YPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEV 542
           YPFLAY  +P  IDINYALF  TEGIYDPKTDLHYDNMLDAQIDAAY AL+DAG+K MEV
Sbjct: 209 YPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEV 268

Query: 543 IVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENL 602
           ++TETGWAS+GD +E AAT  NA+TYNYNLRKRLAKKKGTPLRPK VLKAYIFALFNEN 
Sbjct: 269 MITETGWASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENS 328

Query: 603 KWGPTSERNYGLFKPDGSISYDIGFQGLQSSS 635
           K G +SE ++GLFKPDG+ISYDIGF  L+S S
Sbjct: 329 KPGKSSETHFGLFKPDGTISYDIGFNNLKSDS 360

[Close]

BLAST of MS003732.1 vs. TAIR 10
Match: AT3G15800.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 478.8 bits (1231), Expect = 6.8e-135
Identity = 227/340 (66.76%), Postives = 281/340 (82.65%), Query Frame = 0


Query: 297 ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 356
           +S T  AF GTYG+NYGRIA+N+PSPD V TLL++AKI+N RIYDADHSVL AF GTG+E
Sbjct: 32  SSQTAVAFIGTYGVNYGRIADNLPSPDAVATLLKSAKIRNTRIYDADHSVLTAFRGTGIE 91

Query: 357 IVISIPNEYLKDMSANEDRALNWVKQNVQPFL-PETHICGIAIGNEVLGGSDYGLWSALL 416
           I++ + NE+LKD+S  EDRA+NW+K+NV+PF+   T I GIA+GNE+LGG+D GLW ALL
Sbjct: 92  IIVGLGNEFLKDISVGEDRAMNWIKENVEPFIRGGTKISGIAVGNEILGGTDIGLWEALL 151

Query: 417 GAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIG 476
            AAKNVY+A+++L L  ++++++ HS+AVF+NS+PPSSC F D+VA +MKPLL FF QI 
Sbjct: 152 PAAKNVYSALRRLGLHNVVEVSSPHSEAVFANSYPPSSCTFRDDVAPFMKPLLAFFWQIQ 211

Query: 477 SPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDA 536
           SPF +NAYPFLAY SDP  IDINYALF   +GI DPKT LHYDNM DA +DA+YAALE A
Sbjct: 212 SPFYINAYPFLAYKSDPITIDINYALFEHNKGILDPKTKLHYDNMFDAMVDASYAALEKA 271

Query: 537 GYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIF 596
           GY  + VIV+ETGWAS+GD +E  A+V NA+TYN NLRKRL K+KGTP RP  V++AY+F
Sbjct: 272 GYTKVPVIVSETGWASKGDADEPGASVKNARTYNRNLRKRLQKRKGTPYRPDMVVRAYVF 331

Query: 597 ALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSA 636
           ALFNEN K GPTSERN+GLFKPDG+I+YDIG  GL+SSSA
Sbjct: 332 ALFNENSKPGPTSERNFGLFKPDGTIAYDIGLTGLKSSSA 371

[Close]

BLAST of MS003732.1 vs. TAIR 10
Match: AT2G26600.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 1.1e-116
Identity = 203/266 (76.32%), Postives = 229/266 (86.09%), Query Frame = 0


Query: 369 MSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYNAVKKL 428
           MS+N D A +WVK+N+Q FLP+T I GIAIGNEVLGG D  L  ALLGAAKNVYNA+KK+
Sbjct: 1   MSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVYNALKKM 60

Query: 429 NLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAYPFLAY 488
           NL+  +QITTAHSQAVFS+S+PPSSC F +NV Q+MKPLLEFF QIGSPFCLNAYPFLAY
Sbjct: 61  NLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAY 120

Query: 489 MSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVIVTETG 548
             +P  IDINYALF  TEGIYDPKTDLHYDNMLDAQIDAAY AL+DAG+K MEV++TETG
Sbjct: 121 TYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEVMITETG 180

Query: 549 WASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENLKWGPTS 608
           WAS+GD +E AAT  NA+TYNYNLRKRLAKKKGTPLRPK VLKAYIFALFNEN K G +S
Sbjct: 181 WASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENSKPGKSS 240

Query: 609 ERNYGLFKPDGSISYDIGFQGLQSSS 635
           E ++GLFKPDG+ISYDIGF  L+S S
Sbjct: 241 ETHFGLFKPDGTISYDIGFNNLKSDS 266

[Close]

BLAST of MS003732.1 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 2.7e-91
Identity = 157/321 (48.91%), Postives = 223/321 (69.47%), Query Frame = 0


Query: 309 GINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIPNEYLKD 368
           GINYG+IANN+PSP +V  LLR+  I  V++YDAD +VL +FS + ++ +I + NEYL++
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 369 MSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYNAVKKL 428
           MS +  +A +W++Q ++P + +T I  I +GNE+   +D+ L  +LL A K+VY A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 429 NLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAYPFLAY 488
            L+  + +T+AHS  + S S+PPSS  F +   QY++PLL+F SQI SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 489 MSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVIVTETG 548
              P  + + Y LF   +G+ DP T+LHYDNML AQ+DA Y+A++  G+ D+EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 549 WASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENLKWGPTS 608
           W S+GD+NE  A+  NA  YN NL K + ++KGTP +    +  Y+FALFNENLK GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 609 ERNYGLFKPDGSISYDIGFQG 630
           ERNYGLF PDG   Y++G QG
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

[Close]

BLAST of MS003732.1 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 2.7e-91
Identity = 157/321 (48.91%), Postives = 223/321 (69.47%), Query Frame = 0


Query: 309 GINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLEIVISIPNEYLKD 368
           GINYG+IANN+PSP +V  LLR+  I  V++YDAD +VL +FS + ++ +I + NEYL++
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 369 MSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYGLWSALLGAAKNVYNAVKKL 428
           MS +  +A +W++Q ++P + +T I  I +GNE+   +D+ L  +LL A K+VY A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 429 NLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLEFFSQIGSPFCLNAYPFLAY 488
            L+  + +T+AHS  + S S+PPSS  F +   QY++PLL+F SQI SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 489 MSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAYAALEDAGYKDMEVIVTETG 548
              P  + + Y LF   +G+ DP T+LHYDNML AQ+DA Y+A++  G+ D+EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 549 WASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNVLKAYIFALFNENLKWGPTS 608
           W S+GD+NE  A+  NA  YN NL K + ++KGTP +    +  Y+FALFNENLK GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 609 ERNYGLFKPDGSISYDIGFQG 630
           ERNYGLF PDG   Y++G QG
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

The following BLAST results are available for this feature:

BLAST of MS003732.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144806.1	7.2e-195	99.42	glucan endo-1,3-beta-glucosidase 14-like isoform X1 [Momordica charantia]	[more]
KAA0050153.1	5.2e-185	93.35	glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo var. makuwa] >TYK06409.1 ...	[more]
KAE8637190.1	2.0e-184	92.77	hypothetical protein CSA_021703 [Cucumis sativus]	[more]
XP_008443979.1	1.8e-182	93.81	PREDICTED: glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo]	[more]
XP_031745641.1	7.0e-182	93.22	glucan endo-1,3-beta-glucosidase 14-like [Cucumis sativus]	[more]

BLAST of MS003732.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQG9	3.8e-90	48.91	Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500...	[more]
Q8L868	1.1e-89	50.15	Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860...	[more]
Q9FHX5	2.9e-82	43.47	Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100...	[more]
Q9M069	5.7e-78	44.79	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q9FJU9	6.1e-72	40.98	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of MS003732.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CUI0	3.5e-195	99.42	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014401 ...	[more]
A0A5A7U2L6	2.5e-185	93.35	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B8U7	8.9e-183	93.81	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487436 PE=3 SV...	[more]
A0A6J1JHR0	8.6e-178	91.25	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485203 PE=...	[more]
A0A6J1HB81	8.6e-178	90.99	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461741 P...	[more]

BLAST of MS003732.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26600.1	9.2e-148	75.60	Glycosyl hydrolase superfamily protein	[more]
AT3G15800.1	6.8e-135	66.76	Glycosyl hydrolase superfamily protein	[more]
AT2G26600.2	1.1e-116	76.32	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.1	2.7e-91	48.91	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.2	2.7e-91	48.91	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001739	Methyl-CpG DNA binding	PFAM	PF01429	MBD	coord: 9..51 e-value: 1.8E-4 score: 21.2
IPR001739	Methyl-CpG DNA binding	PROSITE	PS50982	MBD	coord: 1..53 score: 9.313004
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 307..632 e-value: 1.9E-116 score: 390.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..118
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..235
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..64
None	No IPR available	PANTHER	PTHR32227:SF129	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 298..635
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 309..627 e-value: 2.2E-81 score: 273.9
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 298..635
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 308..626
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 9..69

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS003732	MS003732	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS003732.1-cds	MS003732.1-cds-scaffold127:50663..51711	CDS
MS003732.1-cds	MS003732.1-cds-scaffold127:54322..55177	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS003732.1	MS003732.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds