MS003102.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS003102.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionpre-rRNA-processing protein ESF2
Locationscaffold234: 422937 .. 424958 (+)
Sequence length1164
RNA-Seq ExpressionMS003102.1
SyntenyMS003102.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCAAGGAGGAGATCTGTGAAGTTAATTTAACGACTGATCTCAGCCCAGAATCTAGTAAGAAAAGAGCAAAAAGAAGTAAAAACAAAAAGAAGCAGTTGTTGACTGAAGGTGTAGATGTCATGAAAATGAAAGAAGTAAGATCTGTTCAGGTGGAGGAAAGCAACATAAACTCTGATGGTGACAAAGTAAAAAAGAAGAAAAAAAGAAAGAAGAGTGCTAAACAATGTGTAGAAGAGAACTCTATCAAGGCTGAGGCAGGAGAAATTGAAAGTAAAGCAGAGAGTTCTATGAAAGAGGGTGATCCTTTCTCTGATGGTGGAGGTGAGAAAGATGCAGCTTTTGAAAAGAAGCAAATTCCACTGGAGCATGCTGAGGATGCGAGGGCAGACAGTATTGTTAGAGAAAACTCTGGAGAGAAGATTCTAGAGTCTAACAATGGAAAATCAGATCAAAGGATAAAGAGGAAGAAACGGCTTTTGAAGGAATCTGCAAAAGCAAATATGTGTGGGATTTGTTACTTGAGCCGAGTTCCTCCACATATGGATCCATTGAAACTTCGACAGATCCTCTCACATTATGGGGAGATACAAAGAATTTATCTTGCACCCGAGGGTAATGAATAATAATGCATAATGTTTTCTTTGTCTAATTGTTTTTCTGTTGCTTCAAAATTTTGATTGCATATTTTTTCTTTCTTTAGCTCCATTGCTATCATTTTAGTTTAGATGTCCATGATCTAATTGAAGGTTACTTTTGTATTCATGTTCAGACGCTGCAAATCAAGTACAACGAAAGCGTGCAGGAGGGTTTCGAGGGCAATTATTTTCAGAAGGGTATACTCACACTCCTTAGTAACTTTTGTTTTTTTATTTTATTGAAGCATACTCTTGTCACTACTTGGCTTGTCGAAGGGCTTGGTTCTAGTTCCTGATGTAGTATTCTCCTTATACGTGCTCTATGCAGATGGGTTGAATTTACTGATAAAAGAGTTGCGAAGAAGGTTGCTACTATGTTAAATGGTGAACAAATAGGTAACTTTTTATTTAATATGTTCGTGTTTGTACCTGATATCCTAGCTTGATCCATGATTTCTTCTTTCACTTTGGAGGATGATTCATAATAAGCTTTTCAATGATGATAGACACTAAATTGGGATGTTCGATAATGCTCTATTCAGCTCAGAATAGTGAATTAGTTCTTCTGTGGCATGCATAACGTGCTTCGTAGTCTATTTAATTGGGGTTATGAACATCTTAAAATATCATTATGTTCATAGTTGTTTTTCTCTACCTGTTGTGCATGTATTAGGAGGAAGGAAGAGGTCATCTTTCTACTATGATCTCTGGAATATCAAGTATTTGAGTAAATTCAAGTGGGATGATCTTACTGAAGAAACTGGTACACACCTCTAAGACTTGATCCTGCCCCATTCGCCATGTTGCTTACAAAACTGTTACTGATGTTGAACGTTTCTGCAGCTTACAAGCATGCTATACGGGAGCAGAAACTAGCTTTGGAAATTTCTGCCGCTAAGAGGGAGAGGGATTTCTATCTTTCTAAAGTTGATAAATCACGCGCATTGAATTCCATTGAAGAACGGCTGAAGAAGGTACATCTAACAAGCCTTATTGGCACATAACATATTACTATCTCAGCATGCATTCTTAACAAATTAGGACTCGTGGGGATTCTCCTATCCCAGCTGGGATTTGCTTTGGAAATTTGCTGTCAGTTGAGTTTGTTCCTCATAGTGTAGTATAAACTATAAGCGTTGTCCATGCTTTGGAAATATAAACATTGCCCTCTTTTTATATGGCCTACAGAAGCAGAAGTTGCGAGAAGATTCAGAAATGAGTTCTGAACCTGTTGTTAACCAGAAACTTCCAAAACTGATCCGCAACTTCCCACAAACACAACCAGTTGCAGATTCTGCAGTAGAAAACAAACAGAGACTGTCTAAAAATATCCTGGCTAGGGTAAGTGATAAGTTATCATATGATTTACCTATATTCTGTCTG

mRNA sequence

ATGCCCAAGGAGGAGATCTGTGAAGTTAATTTAACGACTGATCTCAGCCCAGAATCTAGTAAGAAAAGAGCAAAAAGAAGTAAAAACAAAAAGAAGCAGTTGTTGACTGAAGGTGTAGATGTCATGAAAATGAAAGAAGTAAGATCTGTTCAGGTGGAGGAAAGCAACATAAACTCTGATGGTGACAAAGTAAAAAAGAAGAAAAAAAGAAAGAAGAGTGCTAAACAATGTGTAGAAGAGAACTCTATCAAGGCTGAGGCAGGAGAAATTGAAAGTAAAGCAGAGAGTTCTATGAAAGAGGGTGATCCTTTCTCTGATGGTGGAGGTGAGAAAGATGCAGCTTTTGAAAAGAAGCAAATTCCACTGGAGCATGCTGAGGATGCGAGGGCAGACAGTATTGTTAGAGAAAACTCTGGAGAGAAGATTCTAGAGTCTAACAATGGAAAATCAGATCAAAGGATAAAGAGGAAGAAACGGCTTTTGAAGGAATCTGCAAAAGCAAATATGTGTGGGATTTGTTACTTGAGCCGAGTTCCTCCACATATGGATCCATTGAAACTTCGACAGATCCTCTCACATTATGGGGAGATACAAAGAATTTATCTTGCACCCGAGGACGCTGCAAATCAAGTACAACGAAAGCGTGCAGGAGGGTTTCGAGGGCAATTATTTTCAGAAGGATGGGTTGAATTTACTGATAAAAGAGTTGCGAAGAAGGTTGCTACTATGTTAAATGGTGAACAAATAGGAGGAAGGAAGAGGTCATCTTTCTACTATGATCTCTGGAATATCAAGTATTTGAGTAAATTCAAGTGGGATGATCTTACTGAAGAAACTGCTTACAAGCATGCTATACGGGAGCAGAAACTAGCTTTGGAAATTTCTGCCGCTAAGAGGGAGAGGGATTTCTATCTTTCTAAAGTTGATAAATCACGCGCATTGAATTCCATTGAAGAACGGCTGAAGAAGAAGCAGAAGTTGCGAGAAGATTCAGAAATGAGTTCTGAACCTGTTGTTAACCAGAAACTTCCAAAACTGATCCGCAACTTCCCACAAACACAACCAGTTGCAGATTCTGCAGTAGAAAACAAACAGAGACTGTCTAAAAATATCCTGGCTAGGGTAAGTGATAAGTTATCATATGATTTACCTATATTCTGTCTG

Coding sequence (CDS)

ATGCCCAAGGAGGAGATCTGTGAAGTTAATTTAACGACTGATCTCAGCCCAGAATCTAGTAAGAAAAGAGCAAAAAGAAGTAAAAACAAAAAGAAGCAGTTGTTGACTGAAGGTGTAGATGTCATGAAAATGAAAGAAGTAAGATCTGTTCAGGTGGAGGAAAGCAACATAAACTCTGATGGTGACAAAGTAAAAAAGAAGAAAAAAAGAAAGAAGAGTGCTAAACAATGTGTAGAAGAGAACTCTATCAAGGCTGAGGCAGGAGAAATTGAAAGTAAAGCAGAGAGTTCTATGAAAGAGGGTGATCCTTTCTCTGATGGTGGAGGTGAGAAAGATGCAGCTTTTGAAAAGAAGCAAATTCCACTGGAGCATGCTGAGGATGCGAGGGCAGACAGTATTGTTAGAGAAAACTCTGGAGAGAAGATTCTAGAGTCTAACAATGGAAAATCAGATCAAAGGATAAAGAGGAAGAAACGGCTTTTGAAGGAATCTGCAAAAGCAAATATGTGTGGGATTTGTTACTTGAGCCGAGTTCCTCCACATATGGATCCATTGAAACTTCGACAGATCCTCTCACATTATGGGGAGATACAAAGAATTTATCTTGCACCCGAGGACGCTGCAAATCAAGTACAACGAAAGCGTGCAGGAGGGTTTCGAGGGCAATTATTTTCAGAAGGATGGGTTGAATTTACTGATAAAAGAGTTGCGAAGAAGGTTGCTACTATGTTAAATGGTGAACAAATAGGAGGAAGGAAGAGGTCATCTTTCTACTATGATCTCTGGAATATCAAGTATTTGAGTAAATTCAAGTGGGATGATCTTACTGAAGAAACTGCTTACAAGCATGCTATACGGGAGCAGAAACTAGCTTTGGAAATTTCTGCCGCTAAGAGGGAGAGGGATTTCTATCTTTCTAAAGTTGATAAATCACGCGCATTGAATTCCATTGAAGAACGGCTGAAGAAGAAGCAGAAGTTGCGAGAAGATTCAGAAATGAGTTCTGAACCTGTTGTTAACCAGAAACTTCCAAAACTGATCCGCAACTTCCCACAAACACAACCAGTTGCAGATTCTGCAGTAGAAAACAAACAGAGACTGTCTAAAAATATCCTGGCTAGGGTAAGTGATAAGTTATCATATGATTTACCTATATTCTGTCTG

Protein sequence

MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSDGDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQIPLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPPHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADSAVENKQRLSKNILARVSDKLSYDLPIFCL
Homology
BLAST of MS003102.1 vs. NCBI nr
Match: XP_022134500.1 (pre-rRNA-processing protein ESF2 [Momordica charantia])

HSP 1 Score: 695.3 bits (1793), Expect = 3.0e-196
Identity = 371/375 (98.93%), Postives = 371/375 (98.93%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD
Sbjct: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
           GDKVKKKKK KKSAKQCVEENSIKAEAGEIESKAESSMKEG PFSDGGGEKDAAFEKKQI
Sbjct: 61  GDKVKKKKKGKKSAKQCVEENSIKAEAGEIESKAESSMKEGGPFSDGGGEKDAAFEKKQI 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP 180
           PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP
Sbjct: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP 180

Query: 181 HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV 240
           HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV
Sbjct: 181 HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV 240

Query: 241 ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE 300
           ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE
Sbjct: 241 ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE 300

Query: 301 RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADSA 360
           RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVV QKLPKLIRNFPQTQPVADSA
Sbjct: 301 RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVYQKLPKLIRNFPQTQPVADSA 360

Query: 361 VENKQRLSKNILARV 376
           VENKQRLSKNILA V
Sbjct: 361 VENKQRLSKNILAGV 375

BLAST of MS003102.1 vs. NCBI nr
Match: XP_038891142.1 (pre-rRNA-processing protein ESF2 [Benincasa hispida])

HSP 1 Score: 562.4 bits (1448), Expect = 3.1e-156
Identity = 307/376 (81.65%), Postives = 334/376 (88.83%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           M KEE  EV+LTTD SP+SSKKRAKR KNK KQLL E  D+M+MKEV SVQ+ ESNIN D
Sbjct: 1   MSKEESFEVDLTTDCSPKSSKKRAKRRKNKNKQLLMEDADIMEMKEVGSVQLGESNINFD 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
            DKVKKKKKRKKS +QCVEE+S+K EAGE E++AE+ MKEG  FS+GG EK   FEK++I
Sbjct: 61  SDKVKKKKKRKKSPQQCVEESSVKDEAGEYENRAETFMKEGSTFSNGGSEK--FFEKRKI 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLKESAKANMCGICYLSRVP 180
            LE AE+ R+ SIVRENSGEKILESNNGK+DQR IKRKKRLLKE+  A+M GICYLSRVP
Sbjct: 121 ALEDAENERSSSIVRENSGEKILESNNGKADQRNIKRKKRLLKETTNADMRGICYLSRVP 180

Query: 181 PHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKK 240
           PHMDPLKLRQILS YGEIQRIYLAPEDAA+QVQRKR+GGFRGQ FSEGWVEFTDKRVAKK
Sbjct: 181 PHMDPLKLRQILSQYGEIQRIYLAPEDAASQVQRKRSGGFRGQFFSEGWVEFTDKRVAKK 240

Query: 241 VATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300
           VA +LNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR
Sbjct: 241 VANILNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300

Query: 301 ERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADS 360
           ERDFYL+KVDKSRALNSIEERLKKKQKLREDSEM S    +QKLPKLIR+FPQTQPVADS
Sbjct: 301 ERDFYLAKVDKSRALNSIEERLKKKQKLREDSEMKSTLADSQKLPKLIRSFPQTQPVADS 360

Query: 361 AVENKQRLSKNILARV 376
            V+NKQRLS N+LA V
Sbjct: 361 TVQNKQRLSTNVLAGV 374

BLAST of MS003102.1 vs. NCBI nr
Match: XP_022922239.1 (pre-rRNA-processing protein ESF2 [Cucurbita moschata] >XP_022922247.1 pre-rRNA-processing protein ESF2 [Cucurbita moschata] >KAG7033078.1 esf2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543.1 bits (1398), Expect = 1.9e-150
Identity = 304/376 (80.85%), Postives = 324/376 (86.17%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           MPKEE  EV LTTD SPESSKK  K SK KKKQLL EGVDVM+     S ++ ESNINS+
Sbjct: 1   MPKEESFEVGLTTDCSPESSKK-LKGSKKKKKQLLVEGVDVMERNVEGSFELWESNINSE 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
              VK KKKRKKS  QCV ENSI+AEAGE E KAE S+KEG P SDGG EK+   E++Q 
Sbjct: 61  ---VKNKKKRKKSTIQCVVENSIQAEAGEYEIKAERSVKEGSPLSDGGSEKNEILEERQF 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLKESAKANMCGICYLSRVP 180
            LE+ E+ RADSIVRE SGEKILESNNGK+DQR IKRKKRLLKE A A+M GICYLSRVP
Sbjct: 121 ALENDENERADSIVRETSGEKILESNNGKTDQRNIKRKKRLLKEVANADMRGICYLSRVP 180

Query: 181 PHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKK 240
           PHMDPLKLRQILS YGEIQRIYLAPEDAANQV+RKRAGGFRGQ FSEGWVEFTDKRVAKK
Sbjct: 181 PHMDPLKLRQILSQYGEIQRIYLAPEDAANQVKRKRAGGFRGQFFSEGWVEFTDKRVAKK 240

Query: 241 VATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300
           VA MLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQK+ALEISAAKR
Sbjct: 241 VANMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKMALEISAAKR 300

Query: 301 ERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADS 360
           ERDFYLSKVDKSRAL SIEERLKKKQKLREDSEM+SE   +QKLPKLIRNFPQTQPVADS
Sbjct: 301 ERDFYLSKVDKSRALKSIEERLKKKQKLREDSEMNSELADSQKLPKLIRNFPQTQPVADS 360

Query: 361 AVENKQRLSKNILARV 376
           AV++K +LS N LA V
Sbjct: 361 AVQDKPKLSTNFLAGV 372

BLAST of MS003102.1 vs. NCBI nr
Match: XP_022990094.1 (pre-rRNA-processing protein ESF2 [Cucurbita maxima] >XP_022990095.1 pre-rRNA-processing protein ESF2 [Cucurbita maxima])

HSP 1 Score: 542.3 bits (1396), Expect = 3.3e-150
Identity = 303/376 (80.59%), Postives = 324/376 (86.17%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           MPKEE  EV+LTTD SPESSKK  K SK KKKQLL EGVDVM+     SV++ ESNINS+
Sbjct: 1   MPKEESFEVDLTTDCSPESSKK-LKGSKKKKKQLLVEGVDVMERNVEGSVELGESNINSE 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
              VK KKKR+KS KQCV ENSI+AEAGE E KAE S+KEG P SDGG EK+   E++Q 
Sbjct: 61  ---VKNKKKRRKSTKQCVVENSIQAEAGEYEIKAERSVKEGSPLSDGGSEKNEILEERQF 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLKESAKANMCGICYLSRVP 180
            LE  E+  ADSIVRE SGEKILESNNGK+DQR IKRKKRLLKE A A+M GICYLSRVP
Sbjct: 121 ALEDDENESADSIVRETSGEKILESNNGKTDQRNIKRKKRLLKEVANADMRGICYLSRVP 180

Query: 181 PHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKK 240
           PHMDPLKLRQILS YGEIQRIYLAPEDAANQV+RKRAGGFRGQ FSEGWVEFTDKRVAKK
Sbjct: 181 PHMDPLKLRQILSQYGEIQRIYLAPEDAANQVKRKRAGGFRGQFFSEGWVEFTDKRVAKK 240

Query: 241 VATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300
           VA MLNGE IGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQK+ALEISAAKR
Sbjct: 241 VANMLNGEPIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKMALEISAAKR 300

Query: 301 ERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADS 360
           ERDFYLSKVDKSRAL SIEERLKKKQKLREDSEM+SE   +QKLPKLIRNFPQTQPVADS
Sbjct: 301 ERDFYLSKVDKSRALKSIEERLKKKQKLREDSEMNSELADSQKLPKLIRNFPQTQPVADS 360

Query: 361 AVENKQRLSKNILARV 376
           AV++K +LS N LA V
Sbjct: 361 AVQDKPKLSTNFLAGV 372

BLAST of MS003102.1 vs. NCBI nr
Match: XP_023529204.1 (pre-rRNA-processing protein ESF2 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023529919.1 pre-rRNA-processing protein ESF2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.8 bits (1392), Expect = 9.6e-150
Identity = 302/376 (80.32%), Postives = 323/376 (85.90%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           MPKEE  EVNLTTD SPESSKK  K SK KKKQLL EGVDVM+     SV++ ESNINS+
Sbjct: 1   MPKEESFEVNLTTDCSPESSKK-LKGSKKKKKQLLVEGVDVMERNVEGSVELWESNINSE 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
              VK KKKRKKS KQCV ENSI+AEAGE E KAE S+KEG P SDGG EK+   E++Q 
Sbjct: 61  ---VKNKKKRKKSTKQCVVENSIQAEAGEYEIKAERSVKEGSPLSDGGSEKNEILEERQF 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLKESAKANMCGICYLSRVP 180
             E  E+ RADSIVRE SGEKILESNNGK+DQR +KRKKRLLKE A A+M GICYLSRVP
Sbjct: 121 AFEDDENERADSIVRETSGEKILESNNGKTDQRNMKRKKRLLKEVANADMRGICYLSRVP 180

Query: 181 PHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKK 240
           PHMDPLKLRQILS YGEIQRIYLAPEDAANQV+RKRAGGFRGQ FSEGWVEFTDKRVAKK
Sbjct: 181 PHMDPLKLRQILSQYGEIQRIYLAPEDAANQVKRKRAGGFRGQFFSEGWVEFTDKRVAKK 240

Query: 241 VATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300
           VA  LNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQK+ALEISAAKR
Sbjct: 241 VANTLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKMALEISAAKR 300

Query: 301 ERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADS 360
           ERDFYLSKVDKSRAL SIEERLKKKQ LREDSEM+SE   +QKLPKLIRNFPQTQPVA+S
Sbjct: 301 ERDFYLSKVDKSRALKSIEERLKKKQNLREDSEMNSELADSQKLPKLIRNFPQTQPVANS 360

Query: 361 AVENKQRLSKNILARV 376
           AV++K +LS N LA V
Sbjct: 361 AVQDKPKLSTNFLAGV 372

BLAST of MS003102.1 vs. ExPASy Swiss-Prot
Match: Q4HZ47 (Pre-rRNA-processing protein ESF2 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=ESF2 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.0e-37
Identity = 104/301 (34.55%), Postives = 163/301 (54.15%), Query Frame = 0

Query: 79  EENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQIPLEHAEDARADSIVRENS 138
           E++  K   G    K ES   E DP SD G + +    +K       ED + D   +   
Sbjct: 27  EDDMQKGGRGAKRRKVESD-DEDDPGSDIGSDDEDEATEKDSKKTQEEDKKEDQPEKPKR 86

Query: 139 GEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPPHMDPLKLRQILSHYGEIQ 198
             K   S       R   KK L+   A     G+ YLSR+PP M P KLR +L  YG I 
Sbjct: 87  KSKDTTSTELPDVTRTLTKKNLVASEAAIKKSGVVYLSRIPPFMKPAKLRSLLEPYGTIN 146

Query: 199 RIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQIGGRKRSSFY 258
           RI+LAPED A+  +R RAGG + + ++EGWVEFT K+ AK V  +LN   IGG+K S ++
Sbjct: 147 RIFLAPEDPASHARRVRAGGNKKRSYTEGWVEFTKKKDAKAVCDLLNARTIGGKKGSYYH 206

Query: 259 YDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVDKSRALNSIE 318
            DLWN+ YL  FKW +LTE+ A ++A R  ++  EIS + +E   ++  V+K++ L+ + 
Sbjct: 207 DDLWNLLYLKGFKWHNLTEQIAAENAERSSRMRAEISKSTKENKEFVRNVEKAKMLDGMA 266

Query: 319 ERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADSAVENKQRLSKNILARVSDK 378
            + K K++    +E  +E   ++ + ++ R+F Q  P+A   ++ + + ++  + RV  K
Sbjct: 267 VKAKAKKR---KAETHTEGQEDESVRQVKRSFKQV-PLAKKKMDGEDQPAE--VTRVLSK 320

Query: 379 L 380
           +
Sbjct: 327 I 320

BLAST of MS003102.1 vs. ExPASy Swiss-Prot
Match: Q6BSS5 (Pre-rRNA-processing protein ESF2 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=ESF2 PE=3 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.5e-36
Identity = 83/230 (36.09%), Postives = 143/230 (62.17%), Query Frame = 0

Query: 147 NGKSDQRIKRKK----RLLKESAKANMCGICYLSRVPPHMDPLKLRQILSHYGEIQRIYL 206
           N K  + I  KK    +L KE  K    G+CYLS++PP+M P KLR +LS +G+I R++L
Sbjct: 68  NSKQAKNINLKKLTPEQLAKEQKKIKKTGVCYLSKIPPYMKPAKLRSVLSRFGKIDRLFL 127

Query: 207 APEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQIGGRKRSSFYYDLW 266
            PED +   +R + GG + + ++ GWVEF +K+ AK  A  LNG ++GG+K S +Y D+ 
Sbjct: 128 KPEDNSTYTKRVKYGGNKKKNYTAGWVEFINKKDAKLCAGTLNGNKLGGKKSSYYYDDII 187

Query: 267 NIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVDKSRALNSIEERLK 326
           NIKYLS FKW DLT++ A ++ IR+ KL++E+S  ++    +++ V+KS+ +N+++ + K
Sbjct: 188 NIKYLSAFKWFDLTQQIAKENEIRQAKLSMELSQQQKLNKSFINNVEKSKMINNMQNKRK 247

Query: 327 KKQ-KLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADSAVENKQRLSKNI 372
            +Q +   D+    E  + + L +  R    T+  A+  +++K + ++ +
Sbjct: 248 ARQAESGADNSNKEESDIRRNLKQ--RKLASTRADAEDELKHKSKPNEKL 295

BLAST of MS003102.1 vs. ExPASy Swiss-Prot
Match: Q59YL9 (Pre-rRNA-processing protein ESF2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ESF2 PE=3 SV=2)

HSP 1 Score: 152.1 bits (383), Expect = 1.3e-35
Identity = 98/280 (35.00%), Postives = 160/280 (57.14%), Query Frame = 0

Query: 71  KKSAKQCVEENSIKAEAGEIESKAES----SMKEGDPFSDGGGEKDAAFE-----KKQIP 130
           ++S  +  +EN+ + +   ++SK  S    +  + + FS+   +KD   E     K +  
Sbjct: 7   RESDFESEDENNFQDKVFNVKSKTSSFFHANTSDNEDFSEDEEDKDEQDEGDTKTKPKDG 66

Query: 131 LEHAEDARADSIVRENSGE------KILESNNGKSDQRIKR--KKRLLKESAKANMCGIC 190
             + ED   D    EN G+       +      K ++++K+   K L KE  +    G+C
Sbjct: 67  KHNFEDIAYDEESEENIGDDEDGNADLSLDGGSKVNKKLKKLTSKELAKEQKRIKRTGVC 126

Query: 191 YLSRVPPHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTD 250
           YLSRVPP+M P KLR +LS +GEI R++L PED +   +R + GG + + F+EGW+EF +
Sbjct: 127 YLSRVPPYMKPAKLRSVLSRFGEIDRLFLKPEDPSVYHKRVKYGGNKKKNFTEGWIEFVN 186

Query: 251 KRVAKKVATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALE 310
           K  AK  A  LNG ++GG+K S +Y D+ NIKYL  FKW DLT++ A ++ +R+ KLA+E
Sbjct: 187 KSDAKLCAATLNGNKLGGKKTSYYYDDIINIKYLPGFKWLDLTQQIAKENEVRQAKLAME 246

Query: 311 ISAAKRERDFYLSKVDKSRALNSIEERLKKKQKLREDSEM 334
           IS  ++    ++S V+KS+ + +I+    KK+K  +D  +
Sbjct: 247 ISQQQKLNKSFVSNVEKSKMIANIQ----KKRKTTDDDNI 282

BLAST of MS003102.1 vs. ExPASy Swiss-Prot
Match: Q6CFT1 (Pre-rRNA-processing protein ESF2 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ESF2 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.3e-35
Identity = 101/281 (35.94%), Postives = 151/281 (53.74%), Query Frame = 0

Query: 91  ESKAESSMKEGDPFSDGGGEKDAAFEKKQIPLEHAEDARADSIVRENSGEKILESNNGKS 150
           E K  +S K+   F +  G+ D   +++       ED   +    E   +K +++++ K+
Sbjct: 53  EEKGRTSKKKTKDFFELAGKTDDGDDEE-------EDEEDEEEEEEEKEKKPVKTSSTKA 112

Query: 151 DQRIKRKKRLLKESAKANMCGICYLSRVPPHMDPLKLRQILSHYGEIQRIYLAPEDAANQ 210
             +   K+ + K + K    G+ Y SR+PP MDP KLR +L  +G + RIYL PED   Q
Sbjct: 113 KTKPMTKEEIEKHNKKIAKTGVVYFSRIPPLMDPGKLRMLLQRFGIVDRIYLVPEDPKAQ 172

Query: 211 VQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQIGGRKRSSFYYDLWNIKYLSKF 270
             R R GG R   ++EGW EFT KR AK  A+ LNG  IGG+K S  Y D+ N KYL KF
Sbjct: 173 AVRIRHGGNRALAYTEGWAEFTKKRYAKTCASTLNGNTIGGKKGSQHYDDIMNAKYLPKF 232

Query: 271 KWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVDKSRALNSIEERLKKKQKLRED 330
           KW DL+E+ A +   R+ +L  EIS A RE   Y+  ++KS+A     ER +++Q+  E 
Sbjct: 233 KWSDLSEQLAQETHNRQARLRTEISQATRENQTYIQSLEKSKA----AERRRQRQE-EEG 292

Query: 331 SEMSSEPVVNQKLPKLIRNFPQTQPVADSAVENKQRLSKNI 372
            E   +P       K+ R+F Q +  +  A E K+ L  ++
Sbjct: 293 GETEEKPA-----KKVHRDFYQGKTHSARAKEGKKELDGSV 316

BLAST of MS003102.1 vs. ExPASy Swiss-Prot
Match: Q75DA3 (Pre-rRNA-processing protein ESF2 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=ESF2 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.8e-35
Identity = 92/240 (38.33%), Postives = 140/240 (58.33%), Query Frame = 0

Query: 116 EKKQIPLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKAN-MCGICY 175
           E K    E    A  D+I  +++ E   E    +  Q   R+ + LK S K+N   G+ Y
Sbjct: 60  ETKAATGEEPATADGDNIAPQSADELRPEERADQLKQERLRRLKKLKASKKSNHKTGVVY 119

Query: 176 LSRVPPHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDK 235
           LS++PP+M P K+RQILS +G++ R++L  ED  +  QR + GG +  +F EGW EF  K
Sbjct: 120 LSKIPPYMKPAKMRQILSRFGDLDRLFLKREDEHSHRQRVKGGGNKKVMFREGWAEFIRK 179

Query: 236 RVAKKVATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEI 295
           + AK  A  LNG  IGG+K + ++ D+ N+KYLS FKW DLTE+ A ++ +R+ KL LEI
Sbjct: 180 KDAKLCAETLNGNIIGGKKGNFYHDDVMNVKYLSGFKWADLTEQIARENDVRQSKLQLEI 239

Query: 296 SAAKRERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQ 355
           S A +    ++  V+KS+ LN+I  +  K +   E +E S  PV  +K+     + P++Q
Sbjct: 240 SQANKLNAEFIRNVEKSKMLNNIRAKKHKDEDSSERTE-SHLPVKQRKVTSNRASAPESQ 298

BLAST of MS003102.1 vs. ExPASy TrEMBL
Match: A0A6J1BYG4 (pre-rRNA-processing protein ESF2 OS=Momordica charantia OX=3673 GN=LOC111006727 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.5e-196
Identity = 371/375 (98.93%), Postives = 371/375 (98.93%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD
Sbjct: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
           GDKVKKKKK KKSAKQCVEENSIKAEAGEIESKAESSMKEG PFSDGGGEKDAAFEKKQI
Sbjct: 61  GDKVKKKKKGKKSAKQCVEENSIKAEAGEIESKAESSMKEGGPFSDGGGEKDAAFEKKQI 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP 180
           PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP
Sbjct: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSRVPP 180

Query: 181 HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV 240
           HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV
Sbjct: 181 HMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKV 240

Query: 241 ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE 300
           ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE
Sbjct: 241 ATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRE 300

Query: 301 RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADSA 360
           RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVV QKLPKLIRNFPQTQPVADSA
Sbjct: 301 RDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVYQKLPKLIRNFPQTQPVADSA 360

Query: 361 VENKQRLSKNILARV 376
           VENKQRLSKNILA V
Sbjct: 361 VENKQRLSKNILAGV 375

BLAST of MS003102.1 vs. ExPASy TrEMBL
Match: A0A6J1E2Q1 (pre-rRNA-processing protein ESF2 OS=Cucurbita moschata OX=3662 GN=LOC111430278 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 9.4e-151
Identity = 304/376 (80.85%), Postives = 324/376 (86.17%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           MPKEE  EV LTTD SPESSKK  K SK KKKQLL EGVDVM+     S ++ ESNINS+
Sbjct: 1   MPKEESFEVGLTTDCSPESSKK-LKGSKKKKKQLLVEGVDVMERNVEGSFELWESNINSE 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
              VK KKKRKKS  QCV ENSI+AEAGE E KAE S+KEG P SDGG EK+   E++Q 
Sbjct: 61  ---VKNKKKRKKSTIQCVVENSIQAEAGEYEIKAERSVKEGSPLSDGGSEKNEILEERQF 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLKESAKANMCGICYLSRVP 180
            LE+ E+ RADSIVRE SGEKILESNNGK+DQR IKRKKRLLKE A A+M GICYLSRVP
Sbjct: 121 ALENDENERADSIVRETSGEKILESNNGKTDQRNIKRKKRLLKEVANADMRGICYLSRVP 180

Query: 181 PHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKK 240
           PHMDPLKLRQILS YGEIQRIYLAPEDAANQV+RKRAGGFRGQ FSEGWVEFTDKRVAKK
Sbjct: 181 PHMDPLKLRQILSQYGEIQRIYLAPEDAANQVKRKRAGGFRGQFFSEGWVEFTDKRVAKK 240

Query: 241 VATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300
           VA MLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQK+ALEISAAKR
Sbjct: 241 VANMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKMALEISAAKR 300

Query: 301 ERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADS 360
           ERDFYLSKVDKSRAL SIEERLKKKQKLREDSEM+SE   +QKLPKLIRNFPQTQPVADS
Sbjct: 301 ERDFYLSKVDKSRALKSIEERLKKKQKLREDSEMNSELADSQKLPKLIRNFPQTQPVADS 360

Query: 361 AVENKQRLSKNILARV 376
           AV++K +LS N LA V
Sbjct: 361 AVQDKPKLSTNFLAGV 372

BLAST of MS003102.1 vs. ExPASy TrEMBL
Match: A0A6J1JP68 (pre-rRNA-processing protein ESF2 OS=Cucurbita maxima OX=3661 GN=LOC111487094 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.6e-150
Identity = 303/376 (80.59%), Postives = 324/376 (86.17%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           MPKEE  EV+LTTD SPESSKK  K SK KKKQLL EGVDVM+     SV++ ESNINS+
Sbjct: 1   MPKEESFEVDLTTDCSPESSKK-LKGSKKKKKQLLVEGVDVMERNVEGSVELGESNINSE 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
              VK KKKR+KS KQCV ENSI+AEAGE E KAE S+KEG P SDGG EK+   E++Q 
Sbjct: 61  ---VKNKKKRRKSTKQCVVENSIQAEAGEYEIKAERSVKEGSPLSDGGSEKNEILEERQF 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLKESAKANMCGICYLSRVP 180
            LE  E+  ADSIVRE SGEKILESNNGK+DQR IKRKKRLLKE A A+M GICYLSRVP
Sbjct: 121 ALEDDENESADSIVRETSGEKILESNNGKTDQRNIKRKKRLLKEVANADMRGICYLSRVP 180

Query: 181 PHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKK 240
           PHMDPLKLRQILS YGEIQRIYLAPEDAANQV+RKRAGGFRGQ FSEGWVEFTDKRVAKK
Sbjct: 181 PHMDPLKLRQILSQYGEIQRIYLAPEDAANQVKRKRAGGFRGQFFSEGWVEFTDKRVAKK 240

Query: 241 VATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300
           VA MLNGE IGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQK+ALEISAAKR
Sbjct: 241 VANMLNGEPIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKMALEISAAKR 300

Query: 301 ERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADS 360
           ERDFYLSKVDKSRAL SIEERLKKKQKLREDSEM+SE   +QKLPKLIRNFPQTQPVADS
Sbjct: 301 ERDFYLSKVDKSRALKSIEERLKKKQKLREDSEMNSELADSQKLPKLIRNFPQTQPVADS 360

Query: 361 AVENKQRLSKNILARV 376
           AV++K +LS N LA V
Sbjct: 361 AVQDKPKLSTNFLAGV 372

BLAST of MS003102.1 vs. ExPASy TrEMBL
Match: A0A1S4E282 (pre-rRNA-processing protein ESF2 OS=Cucumis melo OX=3656 GN=LOC103498123 PE=3 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 6.1e-150
Identity = 300/376 (79.79%), Postives = 329/376 (87.50%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           M KEE  EVNLTTD +PESSKKRAK  KNKKKQLL +GVDVM+MK+VRSVQ+ ESNINSD
Sbjct: 1   MSKEESSEVNLTTDCNPESSKKRAKSRKNKKKQLLMKGVDVMEMKDVRSVQLGESNINSD 60

Query: 61  GDKVKKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEKKQI 120
            DKVKKKKKRKKS +Q +EE+SIK EAGE E++ E+ MKE  P S+ G E    FEK+ I
Sbjct: 61  SDKVKKKKKRKKSTQQRIEESSIKDEAGEYENRTETFMKEASPVSNSGSEN--FFEKRTI 120

Query: 121 PLEHAEDARADSIVRENSGEKILESNNGKSDQR-IKRKKRLLKESAKANMCGICYLSRVP 180
            LE AE+ R D+I+RE S  KILES+NGK D+R IKRKK+LLKE+A A+M GICYLSRVP
Sbjct: 121 ALEDAENERTDNILREKSDRKILESDNGKVDRRKIKRKKQLLKEAANADMRGICYLSRVP 180

Query: 181 PHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKK 240
           PHMDPL+LRQILS YGEIQRIYLAPEDAA+QVQRKRAGGFRGQ FSEGWVEFTDKRVAKK
Sbjct: 181 PHMDPLRLRQILSQYGEIQRIYLAPEDAASQVQRKRAGGFRGQFFSEGWVEFTDKRVAKK 240

Query: 241 VATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300
           VA MLNGE IGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR
Sbjct: 241 VANMLNGEPIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKR 300

Query: 301 ERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVADS 360
           ERD YL+KVDKSRALNSIEERLKKKQKLREDSEM+S    +QKLPKLIR+FPQTQPVAD 
Sbjct: 301 ERDSYLAKVDKSRALNSIEERLKKKQKLREDSEMNSLD-DSQKLPKLIRSFPQTQPVADV 360

Query: 361 AVENKQRLSKNILARV 376
           AV+NKQRLS N+LARV
Sbjct: 361 AVQNKQRLSTNVLARV 373

BLAST of MS003102.1 vs. ExPASy TrEMBL
Match: A0A0A0KUC2 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517890 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 3.1e-146
Identity = 292/378 (77.25%), Postives = 326/378 (86.24%), Query Frame = 0

Query: 1   MPKEEICEVNLTTDLSPESSKKRAKRSKNKKKQLLTEGVDVMKMKEVRSVQVEESNINSD 60
           M  EE  EVNL TD SPES KKRAK  KNK+KQLL +GVDVM+MK+V SVQ+ ESN+N+D
Sbjct: 1   MSNEESSEVNLKTDCSPESRKKRAKSRKNKRKQLLIKGVDVMEMKDVGSVQLGESNMNTD 60

Query: 61  GDKV---KKKKKRKKSAKQCVEENSIKAEAGEIESKAESSMKEGDPFSDGGGEKDAAFEK 120
            DKV   KKKKKRKKS +Q VEE+ IK EAGE E++AE+ MKE  P S+ G E    FEK
Sbjct: 61  SDKVKKKKKKKKRKKSTQQRVEESFIKDEAGEYENRAETFMKEASPVSNSGSE--IFFEK 120

Query: 121 KQIPLEHAEDARADSIVRENSGEKILESNNGKSDQRIKRKKRLLKESAKANMCGICYLSR 180
           ++I LE AE+ R DSI+RENS  KILES+NGK+ ++IKRKK+LLKE+A A+M GICYLSR
Sbjct: 121 RKIALEDAENERTDSILRENSDGKILESDNGKNQRKIKRKKQLLKEAANADMRGICYLSR 180

Query: 181 VPPHMDPLKLRQILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVA 240
           VPPHMDPLKLRQILS +GEIQRIYLAPEDAA+QVQRKRAGGFRGQ FSEGWVEFTDKRVA
Sbjct: 181 VPPHMDPLKLRQILSQHGEIQRIYLAPEDAASQVQRKRAGGFRGQFFSEGWVEFTDKRVA 240

Query: 241 KKVATMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAA 300
           K+VA MLNGE IGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAA
Sbjct: 241 KRVANMLNGEPIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAA 300

Query: 301 KRERDFYLSKVDKSRALNSIEERLKKKQKLREDSEMSSEPVVNQKLPKLIRNFPQTQPVA 360
           KRERDFYL+KVDKSRALNSIEERLKKKQK+REDSEM+S    +QKLPKLIR+FPQTQPVA
Sbjct: 301 KRERDFYLAKVDKSRALNSIEERLKKKQKMREDSEMNSTLDDSQKLPKLIRSFPQTQPVA 360

Query: 361 DSAVENKQRLSKNILARV 376
           D AV+NK RLS N+LA V
Sbjct: 361 DFAVQNKPRLSTNVLAGV 376

BLAST of MS003102.1 vs. TAIR 10
Match: AT3G56510.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 271.6 bits (693), Expect = 1.0e-72
Identity = 143/248 (57.66%), Postives = 190/248 (76.61%), Query Frame = 0

Query: 131 DSIVRENSGEKILESNNGKSDQRIKR-KKRLLKESAKANMCGICYLSRVPPHMDPLKLRQ 190
           D I  E   ++ ++S   K+D++ K+ K++LLKE++KA+  G+CYLSR+PPHMD ++LR 
Sbjct: 11  DGISEEKESKETMKSQ--KADRKKKKLKEKLLKEASKADNRGVCYLSRIPPHMDHVRLRH 70

Query: 191 ILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQI 250
           IL+ YGE+ RIYLAPED+  QV RKRAGGFRGQ FSEGWVEF  K VAK+VA MLNGEQI
Sbjct: 71  ILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVADMLNGEQI 130

Query: 251 GGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVD 310
           GG+K+SS YYD+WNIKYL+KFKWDDLTEE AYK AIREQKL + +SAAKRE+DFYLSK++
Sbjct: 131 GGKKKSSVYYDIWNIKYLTKFKWDDLTEEIAYKSAIREQKLNMVLSAAKREKDFYLSKIE 190

Query: 311 KSRALNSIEERLKKKQKLREDSEMSSE--PVVNQKLPKLIRNFPQTQPVADSAVENKQRL 370
           KSRA+  I+ R++KK+K++E+S  ++E  PV     P+ I  F Q + + +   ++K  L
Sbjct: 191 KSRAMTEIDARMEKKRKIQEESGSNAEAAPVFP---PRAIFQFRQKKSIENETSQSKPGL 250

Query: 371 SKNILARV 376
           S + LA V
Sbjct: 251 STDFLASV 253

BLAST of MS003102.1 vs. TAIR 10
Match: AT3G56510.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 271.6 bits (693), Expect = 1.0e-72
Identity = 143/248 (57.66%), Postives = 190/248 (76.61%), Query Frame = 0

Query: 131 DSIVRENSGEKILESNNGKSDQRIKR-KKRLLKESAKANMCGICYLSRVPPHMDPLKLRQ 190
           D I  E   ++ ++S   K+D++ K+ K++LLKE++KA+  G+CYLSR+PPHMD ++LR 
Sbjct: 11  DGISEEKESKETMKSQ--KADRKKKKLKEKLLKEASKADNRGVCYLSRIPPHMDHVRLRH 70

Query: 191 ILSHYGEIQRIYLAPEDAANQVQRKRAGGFRGQLFSEGWVEFTDKRVAKKVATMLNGEQI 250
           IL+ YGE+ RIYLAPED+  QV RKRAGGFRGQ FSEGWVEF  K VAK+VA MLNGEQI
Sbjct: 71  ILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVADMLNGEQI 130

Query: 251 GGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERDFYLSKVD 310
           GG+K+SS YYD+WNIKYL+KFKWDDLTEE AYK AIREQKL + +SAAKRE+DFYLSK++
Sbjct: 131 GGKKKSSVYYDIWNIKYLTKFKWDDLTEEIAYKSAIREQKLNMVLSAAKREKDFYLSKIE 190

Query: 311 KSRALNSIEERLKKKQKLREDSEMSSE--PVVNQKLPKLIRNFPQTQPVADSAVENKQRL 370
           KSRA+  I+ R++KK+K++E+S  ++E  PV     P+ I  F Q + + +   ++K  L
Sbjct: 191 KSRAMTEIDARMEKKRKIQEESGSNAEAAPVFP---PRAIFQFRQKKSIENETSQSKPGL 250

Query: 371 SKNILARV 376
           S + LA V
Sbjct: 251 STDFLASV 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134500.13.0e-19698.93pre-rRNA-processing protein ESF2 [Momordica charantia][more]
XP_038891142.13.1e-15681.65pre-rRNA-processing protein ESF2 [Benincasa hispida][more]
XP_022922239.11.9e-15080.85pre-rRNA-processing protein ESF2 [Cucurbita moschata] >XP_022922247.1 pre-rRNA-p... [more]
XP_022990094.13.3e-15080.59pre-rRNA-processing protein ESF2 [Cucurbita maxima] >XP_022990095.1 pre-rRNA-pro... [more]
XP_023529204.19.6e-15080.32pre-rRNA-processing protein ESF2 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023... [more]
Match NameE-valueIdentityDescription
Q4HZ473.0e-3734.55Pre-rRNA-processing protein ESF2 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS ... [more]
Q6BSS51.5e-3636.09Pre-rRNA-processing protein ESF2 OS=Debaryomyces hansenii (strain ATCC 36239 / C... [more]
Q59YL91.3e-3535.00Pre-rRNA-processing protein ESF2 OS=Candida albicans (strain SC5314 / ATCC MYA-2... [more]
Q6CFT11.3e-3535.94Pre-rRNA-processing protein ESF2 OS=Yarrowia lipolytica (strain CLIB 122 / E 150... [more]
Q75DA32.8e-3538.33Pre-rRNA-processing protein ESF2 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109... [more]
Match NameE-valueIdentityDescription
A0A6J1BYG41.5e-19698.93pre-rRNA-processing protein ESF2 OS=Momordica charantia OX=3673 GN=LOC111006727 ... [more]
A0A6J1E2Q19.4e-15180.85pre-rRNA-processing protein ESF2 OS=Cucurbita moschata OX=3662 GN=LOC111430278 P... [more]
A0A6J1JP681.6e-15080.59pre-rRNA-processing protein ESF2 OS=Cucurbita maxima OX=3661 GN=LOC111487094 PE=... [more]
A0A1S4E2826.1e-15079.79pre-rRNA-processing protein ESF2 OS=Cucumis melo OX=3656 GN=LOC103498123 PE=3 SV... [more]
A0A0A0KUC23.1e-14677.25RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517890 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT3G56510.11.0e-7257.66RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT3G56510.21.0e-7257.66RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 314..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..50
NoneNo IPR availablePANTHERPTHR12311:SF7ACTIVATOR OF BASAL TRANSCRIPTION 1coord: 122..375
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 108..256
e-value: 7.6E-10
score: 40.9
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 174..253
e-value: 3.5E-5
score: 23.6
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 171..253
score: 8.978701
IPR039119ABT1/Esf2PANTHERPTHR12311ACTIVATOR OF BASAL TRANSCRIPTION 1coord: 122..375
IPR034353ABT1/ESF2, RNA recognition motifCDDcd12263RRM_ABT1_likecoord: 171..268
e-value: 9.32485E-55
score: 173.932
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 165..253

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS003102MS003102gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS003102.1-cdsMS003102.1-cds-scaffold234:422937..423552CDS
MS003102.1-cdsMS003102.1-cds-scaffold234:423710..423773CDS
MS003102.1-cdsMS003102.1-cds-scaffold234:423904..423971CDS
MS003102.1-cdsMS003102.1-cds-scaffold234:424249..424338CDS
MS003102.1-cdsMS003102.1-cds-scaffold234:424419..424549CDS
MS003102.1-cdsMS003102.1-cds-scaffold234:424764..424958CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS003102.1MS003102.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding