MS002153.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS002153.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pentatricopeptide repeat-containing protein
Location	scaffold30: 2800493 .. 2801521 (-)
Sequence length	540
RNA-Seq Expression	MS002153.1
Synteny	MS002153.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTTTGTTTCTTCTACAGTGAATTATAATCGATTGATTTATCAGTTTTGTTCGTTTTCTCTACCGAATGTAGCTCATAGGATTTTATTCGATATGACGGGTAGAGGGCATTGCCCAAATGCTGTTTCATATACTACCTTGATTAATGGATACTGCTGTGTTGGTAATGTACCTGCTGCAAAGAAACTGTTTGATGAAATGCCTGAGAATGACGCGGAGCCTAGTTCTCTTACATATAGTGTTCTAATTCATGGTTTTCTTTGCAAGCAGGATTTCGAAACTGGGAGGGCGTTGATGTGCAAGCTTTGGGAAAAGATAAAAGGGGGAAAAGGATCCCTTCACGAACAATGCTGCTCTTGCGAAGCATCTTATTGATTCTCTGTGCTAAGCAGGTTCTTTCCACGAGGTTTTTTTAATCGTAGAAGATATGCCTCAGGGGCAGAGTGTGCATGAGGAATTTGCTTATGGGCAGATGATAGATTCACTTTGTAAAGCTAGAAGACATCATGGGGCCTCAAGAATTGTATATATAATGAGGAAGAGAGGGTTTAATCCTGGTTTACTCTCGCATAATTCTATTAGCAAGGAATTGAATTTGGGTACTCACCATCTGAACATACAAATAAGGTTCTTGTAGAAGGTCTTTGCCTAGAGATAGATATCCAGGCAAAGGAAGTTTTTCAAATAATGATAGACAAGGAAGGTGTCGACAGAACTTGAATTTACAACATATACTTGAGAGCTATTTGCCTTGCAAATAAGTCAACCGAGCTCTTAAATACATTTGTTGTAATGCTTCAAACCAATTGTCAATCTGATGTTATTACCCTGAATACAGTTATCAAGGGATTTTGTCATATTGGAAGCATTGAAAAAGCTCTAAAGGTATTAAATGATATGATCATTTGTCAATTTTGTGCCCCTGATTATGTGAACTTCACGACTATTATATATGGGTTACTGAATGTTGGGAGGATCCAGGAATCTCTTGATATATTATATAAAGTAATGCGAGAAAAAGGCATTATG

mRNA sequence

GAGTTTGTTTCTTCTACAGTGAATTATAATCGATTGATTTATCAGTTTTGTTCGTTTTCTCTACCGAATGTAGCTCATAGGATTTTATTCGATATGACGGGTAGAGGGCATTGCCCAAATGCTGTTTCATATACTACCTTGATTAATGGATACTGCTGTGTTGGTAATGTACCTGCTGCAAAGAAACTGTTTGATGAAATGCCTGAGAATGACGCGGAGCCTAGTTCTCTTACATATAGTGTTCTAATTCATGCTATTTGCCTTGCAAATAAGTCAACCGAGCTCTTAAATACATTTGTTGTAATGCTTCAAACCAATTGTCAATCTGATGTTATTACCCTGAATACAGTTATCAAGGGATTTTGTCATATTGGAAGCATTGAAAAAGCTCTAAAGGTATTAAATGATATGATCATTTGTCAATTTTGTGCCCCTGATTATGTGAACTTCACGACTATTATATATGGGTTACTGAATGTTGGGAGGATCCAGGAATCTCTTGATATATTATATAAAGTAATGCGAGAAAAAGGCATTATG

Coding sequence (CDS)

GAGTTTGTTTCTTCTACAGTGAATTATAATCGATTGATTTATCAGTTTTGTTCGTTTTCTCTACCGAATGTAGCTCATAGGATTTTATTCGATATGACGGGTAGAGGGCATTGCCCAAATGCTGTTTCATATACTACCTTGATTAATGGATACTGCTGTGTTGGTAATGTACCTGCTGCAAAGAAACTGTTTGATGAAATGCCTGAGAATGACGCGGAGCCTAGTTCTCTTACATATAGTGTTCTAATTCATGCTATTTGCCTTGCAAATAAGTCAACCGAGCTCTTAAATACATTTGTTGTAATGCTTCAAACCAATTGTCAATCTGATGTTATTACCCTGAATACAGTTATCAAGGGATTTTGTCATATTGGAAGCATTGAAAAAGCTCTAAAGGTATTAAATGATATGATCATTTGTCAATTTTGTGCCCCTGATTATGTGAACTTCACGACTATTATATATGGGTTACTGAATGTTGGGAGGATCCAGGAATCTCTTGATATATTATATAAAGTAATGCGAGAAAAAGGCATTATG

Protein sequence

EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAAKKLFDEMPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFCHIGSIEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVMREKGIM

Homology

[Close]

BLAST of MS002153.1 vs. NCBI nr
Match: XP_022134613.1 (LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At3g18020 [Momordica charantia])

HSP 1 Score: 298.1 bits (762), Expect = 5.0e-77
Identity = 178/343 (51.90%), Postives = 180/343 (52.48%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLI+GYCCVGNVPAA
Sbjct: 149 EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLIDGYCCVGNVPAA 208

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           KKLFDEMPENDAEPSSLTYSVLIH                                    
Sbjct: 209 KKLFDEMPENDAEPSSLTYSVLIHGFLCKQDFETGRALMCKLWEKIKGEKDPFTNNAALA 268

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 269 KHLIDSLCQAGSFHEVFLIVEDMPQGQSVHEEFAYGQMIDSLCKARRHHGASRIAYIMRK 328

[Close]

BLAST of MS002153.1 vs. NCBI nr
Match: KAA0058133.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 217.6 bits (553), Expect = 8.6e-53
Identity = 144/360 (40.00%), Postives = 158/360 (43.89%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGH PN VSYT LI+GYC VGNV AA
Sbjct: 196 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHSPNVVSYTALIDGYCRVGNVSAA 255

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMPEND EP+SLTYSVLI+                                    
Sbjct: 256 EKLFDEMPENDVEPNSLTYSVLINGFLYKRDFEAGKALICKLWERMTGEMDSSVNNAAFA 315

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 316 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 375

[Close]

BLAST of MS002153.1 vs. NCBI nr
Match: XP_008453476.1 (PREDICTED: pentatricopeptide repeat-containing protein At3g18020 [Cucumis melo] >XP_008453477.1 PREDICTED: pentatricopeptide repeat-containing protein At3g18020 [Cucumis melo] >XP_008453478.1 PREDICTED: pentatricopeptide repeat-containing protein At3g18020 [Cucumis melo] >XP_016901429.1 PREDICTED: pentatricopeptide repeat-containing protein At3g18020 [Cucumis melo])

HSP 1 Score: 217.6 bits (553), Expect = 8.6e-53
Identity = 144/360 (40.00%), Postives = 158/360 (43.89%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGH PN VSYT LI+GYC VGNV AA
Sbjct: 156 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHSPNVVSYTALIDGYCRVGNVSAA 215

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMPEND EP+SLTYSVLI+                                    
Sbjct: 216 EKLFDEMPENDVEPNSLTYSVLINGFLYKRDFEAGKALICKLWERMTGEMDSSVNNAAFA 275

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 276 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 335

[Close]

BLAST of MS002153.1 vs. NCBI nr
Match: TYK28487.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 217.6 bits (553), Expect = 8.6e-53
Identity = 144/360 (40.00%), Postives = 158/360 (43.89%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGH PN VSYT LI+GYC VGNV AA
Sbjct: 196 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHSPNVVSYTALIDGYCRVGNVSAA 255

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMPEND EP+SLTYSVLI+                                    
Sbjct: 256 EKLFDEMPENDVEPNSLTYSVLINGFLYKRDFEAGKALICKLWERMTGEMDSSVNNAAFA 315

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 316 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 375

[Close]

BLAST of MS002153.1 vs. NCBI nr
Match: XP_011649150.1 (pentatricopeptide repeat-containing protein At3g18020 [Cucumis sativus] >KGN63871.1 hypothetical protein Csa_014101 [Cucumis sativus])

HSP 1 Score: 213.8 bits (543), Expect = 1.2e-51
Identity = 143/360 (39.72%), Postives = 156/360 (43.33%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGHCPN VSYT LI+GYC V NV AA
Sbjct: 156 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHCPNVVSYTALIDGYCRVCNVSAA 215

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMP N  EP+SLTYSVLI+                                    
Sbjct: 216 EKLFDEMPGNYVEPNSLTYSVLINGFLYKRDFETGKALICNLWERMKGELDSSVNNAAFA 275

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 276 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 335

[Close]

BLAST of MS002153.1 vs. ExPASy Swiss-Prot
Match: Q9LSK8 (Pentatricopeptide repeat-containing protein At3g18020 OS=Arabidopsis thaliana OX=3702 GN=At3g18020 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.5e-26
Identity = 72/214 (33.64%), Postives = 105/214 (49.07%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           E V+    Y  +I   C +   + A RI++ M  +G  P   SY  +I+G C  G    A
Sbjct: 302 ESVNVEFAYGHMIDSLCRYRRNHGAARIVYIMKSKGLKPRRTSYNAIIHGLCKDGGCMRA 361

Query: 61  KKLFDEMPENDAEPSSLTYSVL-----------------------------------IHA 120
            +L +E  E +  PS  TY +L                                   +  
Sbjct: 362 YQLLEEGSEFEFFPSEYTYKLLMESLCKELDTGKARNVLELMLRKEGADRTRIYNIYLRG 421

Query: 121 ICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFCHIGSIEKALKVLNDMIICQFCAP 180
           +C+ +  TE+LN  V MLQ +C+ D  TLNTVI G C +G ++ A+KVL+DM+  +FCAP
Sbjct: 422 LCVMDNPTEILNVLVSMLQGDCRPDEYTLNTVINGLCKMGRVDDAMKVLDDMMTGKFCAP 481

[Close]

BLAST of MS002153.1 vs. ExPASy Swiss-Prot
Match: Q9LUR2 (Putative pentatricopeptide repeat-containing protein At3g16710, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g16710 PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.5e-20
Identity = 54/174 (31.03%), Postives = 90/174 (51.72%), Query Frame = 0


Query: 7   VNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAAKKLFDE 66
           V Y  LI+ FC         +I ++M+ +G   N ++YT LI GYC VG    A+++F++
Sbjct: 329 VIYTTLIHGFCKSKRVEDGMKIFYEMSQKGVVANTITYTVLIQGYCLVGRPDVAQEVFNQ 388

Query: 67  MPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFCHIGS 126
           M    A P   TY+VL+  +C   K  + L  F  M +     +++T   +I+G C +G 
Sbjct: 389 MSSRRAPPDIRTYNVLLDGLCCNGKVEKALMIFEYMRKREMDINIVTYTIIIQGMCKLGK 448

Query: 127 IEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVMREKGIM 181
           +E A  +    +  +   P+ + +TT+I G    G I E+ D L+K M+E G +
Sbjct: 449 VEDAFDLFCS-LFSKGMKPNVITYTTMISGFCRRGLIHEA-DSLFKKMKEDGFL 500

[Close]

BLAST of MS002153.1 vs. ExPASy Swiss-Prot
Match: Q9FMF6 (Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g64320 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.5e-20
Identity = 54/172 (31.40%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 3   VSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAAKK 62
           V     YN LIY +    L  +A  +L DM  +G  PN  SYT L++G+C +G +  A  
Sbjct: 386 VPDVCTYNSLIYGYWKEGLVGLALEVLHDMRNKGCKPNVYSYTILVDGFCKLGKIDEAYN 445

Query: 63  LFDEMPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFC 122
           + +EM  +  +P+++ ++ LI A C  ++  E +  F  M +  C+ DV T N++I G C
Sbjct: 446 VLNEMSADGLKPNTVGFNCLISAFCKEHRIPEAVEIFREMPRKGCKPDVYTFNSLISGLC 505

Query: 123 HIGSIEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVM 175
            +  I+ AL +L DM I +    + V + T+I   L  G I+E+  ++ +++
Sbjct: 506 EVDEIKHALWLLRDM-ISEGVVANTVTYNTLINAFLRRGEIKEARKLVNEMV 556

[Close]

BLAST of MS002153.1 vs. ExPASy Swiss-Prot
Match: Q0WPZ6 (Pentatricopeptide repeat-containing protein At2g17140 OS=Arabidopsis thaliana OX=3702 GN=At2g17140 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.6e-20
Identity = 56/195 (28.72%), Postives = 97/195 (49.74%), Query Frame = 0


Query: 5   STVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAAKKLF 64
           S  +YN L+   C   + + A  I+  M   G CP+AV+Y  L++GYC VG V AAK L 
Sbjct: 360 SIYSYNILMDGLCKLGMLSDAKTIVGLMKRNGVCPDAVTYGCLLHGYCSVGKVDAAKSLL 419

Query: 65  DEMPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFCHI 124
            EM  N+  P++ T ++L+H++    + +E       M +     D +T N ++ G C  
Sbjct: 420 QEMMRNNCLPNAYTCNILLHSLWKMGRISEAEELLRKMNEKGYGLDTVTCNIIVDGLCGS 479

Query: 125 GSIEKALKV----------------------LNDMIICQFCAPDYVNFTTIIYGLLNVGR 178
           G ++KA+++                      ++D +I   C PD + ++T++ GL   GR
Sbjct: 480 GELDKAIEIVKGMRVHGSAALGNLGNSYIGLVDDSLIENNCLPDLITYSTLLNGLCKAGR 539

[Close]

BLAST of MS002153.1 vs. ExPASy Swiss-Prot
Match: Q9SXD8 (Pentatricopeptide repeat-containing protein At1g62590 OS=Arabidopsis thaliana OX=3702 GN=At1g62590 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.6e-20
Identity = 63/179 (35.20%), Postives = 95/179 (53.07%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           E V     YN LI  FC  S  ++A  +L  M   G+ P+ V+ ++L+NGYC    +  A
Sbjct: 115 EIVHGLYTYNILINCFCRRSQISLALALLGKMMKLGYEPSIVTLSSLLNGYCHGKRISDA 174

Query: 61  KKLFDEMPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKG 120
             L D+M E    P ++T++ LIH + L NK++E +     M+Q  CQ +++T   V+ G
Sbjct: 175 VALVDQMVEMGYRPDTITFTTLIHGLFLHNKASEAVALVDRMVQRGCQPNLVTYGVVVNG 234

Query: 121 FCHIGSIEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVMREKGI 180
            C  G  + AL +LN M   +  A D V F TII  L     + ++L+ L+K M  KGI
Sbjct: 235 LCKRGDTDLALNLLNKMEAAKIEA-DVVIFNTIIDSLCKYRHVDDALN-LFKEMETKGI 291

[Close]

BLAST of MS002153.1 vs. ExPASy TrEMBL
Match: A0A6J1C2H5 (LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At3g18020 OS=Momordica charantia OX=3673 GN=LOC111006840 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.4e-77
Identity = 178/343 (51.90%), Postives = 180/343 (52.48%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLI+GYCCVGNVPAA
Sbjct: 149 EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLIDGYCCVGNVPAA 208

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           KKLFDEMPENDAEPSSLTYSVLIH                                    
Sbjct: 209 KKLFDEMPENDAEPSSLTYSVLIHGFLCKQDFETGRALMCKLWEKIKGEKDPFTNNAALA 268

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 269 KHLIDSLCQAGSFHEVFLIVEDMPQGQSVHEEFAYGQMIDSLCKARRHHGASRIAYIMRK 328

[Close]

BLAST of MS002153.1 vs. ExPASy TrEMBL
Match: A0A1S3BWE8 (pentatricopeptide repeat-containing protein At3g18020 OS=Cucumis melo OX=3656 GN=LOC103494179 PE=4 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 4.2e-53
Identity = 144/360 (40.00%), Postives = 158/360 (43.89%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGH PN VSYT LI+GYC VGNV AA
Sbjct: 156 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHSPNVVSYTALIDGYCRVGNVSAA 215

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMPEND EP+SLTYSVLI+                                    
Sbjct: 216 EKLFDEMPENDVEPNSLTYSVLINGFLYKRDFEAGKALICKLWERMTGEMDSSVNNAAFA 275

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 276 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 335

[Close]

BLAST of MS002153.1 vs. ExPASy TrEMBL
Match: A0A5D3DXA6 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001220 PE=4 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 4.2e-53
Identity = 144/360 (40.00%), Postives = 158/360 (43.89%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGH PN VSYT LI+GYC VGNV AA
Sbjct: 196 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHSPNVVSYTALIDGYCRVGNVSAA 255

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMPEND EP+SLTYSVLI+                                    
Sbjct: 256 EKLFDEMPENDVEPNSLTYSVLINGFLYKRDFEAGKALICKLWERMTGEMDSSVNNAAFA 315

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 316 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 375

[Close]

BLAST of MS002153.1 vs. ExPASy TrEMBL
Match: A0A5A7UQI7 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004440 PE=4 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 4.2e-53
Identity = 144/360 (40.00%), Postives = 158/360 (43.89%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGH PN VSYT LI+GYC VGNV AA
Sbjct: 196 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHSPNVVSYTALIDGYCRVGNVSAA 255

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMPEND EP+SLTYSVLI+                                    
Sbjct: 256 EKLFDEMPENDVEPNSLTYSVLINGFLYKRDFEAGKALICKLWERMTGEMDSSVNNAAFA 315

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 316 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 375

[Close]

BLAST of MS002153.1 vs. ExPASy TrEMBL
Match: A0A0A0LV25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025030 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 6.0e-52
Identity = 143/360 (39.72%), Postives = 156/360 (43.33%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           EFV S VNYNRLI QFCSFSLPNVAHR+LFDM  RGHCPN VSYT LI+GYC V NV AA
Sbjct: 156 EFVPSIVNYNRLIDQFCSFSLPNVAHRVLFDMKSRGHCPNVVSYTALIDGYCRVCNVSAA 215

Query: 61  KKLFDEMPENDAEPSSLTYSVLIH------------------------------------ 120
           +KLFDEMP N  EP+SLTYSVLI+                                    
Sbjct: 216 EKLFDEMPGNYVEPNSLTYSVLINGFLYKRDFETGKALICNLWERMKGELDSSVNNAAFA 275

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 276 HLVDSLCLVGSFHEVFTIAEDMPQGQSVPEEFAYGQMIDSLCKAKRYHGASRIVYIMRKK 335

[Close]

BLAST of MS002153.1 vs. TAIR 10
Match: AT3G18020.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 1.7e-27
Identity = 72/214 (33.64%), Postives = 105/214 (49.07%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           E V+    Y  +I   C +   + A RI++ M  +G  P   SY  +I+G C  G    A
Sbjct: 302 ESVNVEFAYGHMIDSLCRYRRNHGAARIVYIMKSKGLKPRRTSYNAIIHGLCKDGGCMRA 361

Query: 61  KKLFDEMPENDAEPSSLTYSVL-----------------------------------IHA 120
            +L +E  E +  PS  TY +L                                   +  
Sbjct: 362 YQLLEEGSEFEFFPSEYTYKLLMESLCKELDTGKARNVLELMLRKEGADRTRIYNIYLRG 421

Query: 121 ICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFCHIGSIEKALKVLNDMIICQFCAP 180
           +C+ +  TE+LN  V MLQ +C+ D  TLNTVI G C +G ++ A+KVL+DM+  +FCAP
Sbjct: 422 LCVMDNPTEILNVLVSMLQGDCRPDEYTLNTVINGLCKMGRVDDAMKVLDDMMTGKFCAP 481

[Close]

BLAST of MS002153.1 vs. TAIR 10
Match: AT3G16710.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 54/174 (31.03%), Postives = 90/174 (51.72%), Query Frame = 0


Query: 7   VNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAAKKLFDE 66
           V Y  LI+ FC         +I ++M+ +G   N ++YT LI GYC VG    A+++F++
Sbjct: 329 VIYTTLIHGFCKSKRVEDGMKIFYEMSQKGVVANTITYTVLIQGYCLVGRPDVAQEVFNQ 388

Query: 67  MPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFCHIGS 126
           M    A P   TY+VL+  +C   K  + L  F  M +     +++T   +I+G C +G 
Sbjct: 389 MSSRRAPPDIRTYNVLLDGLCCNGKVEKALMIFEYMRKREMDINIVTYTIIIQGMCKLGK 448

Query: 127 IEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVMREKGIM 181
           +E A  +    +  +   P+ + +TT+I G    G I E+ D L+K M+E G +
Sbjct: 449 VEDAFDLFCS-LFSKGMKPNVITYTTMISGFCRRGLIHEA-DSLFKKMKEDGFL 500

[Close]

BLAST of MS002153.1 vs. TAIR 10
Match: AT5G64320.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 54/172 (31.40%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 3   VSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAAKK 62
           V     YN LIY +    L  +A  +L DM  +G  PN  SYT L++G+C +G +  A  
Sbjct: 386 VPDVCTYNSLIYGYWKEGLVGLALEVLHDMRNKGCKPNVYSYTILVDGFCKLGKIDEAYN 445

Query: 63  LFDEMPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFC 122
           + +EM  +  +P+++ ++ LI A C  ++  E +  F  M +  C+ DV T N++I G C
Sbjct: 446 VLNEMSADGLKPNTVGFNCLISAFCKEHRIPEAVEIFREMPRKGCKPDVYTFNSLISGLC 505

Query: 123 HIGSIEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVM 175
            +  I+ AL +L DM I +    + V + T+I   L  G I+E+  ++ +++
Sbjct: 506 EVDEIKHALWLLRDM-ISEGVVANTVTYNTLINAFLRRGEIKEARKLVNEMV 556

[Close]

BLAST of MS002153.1 vs. TAIR 10
Match: AT1G63130.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 1.9e-21
Identity = 55/176 (31.25%), Postives = 99/176 (56.25%), Query Frame = 0


Query: 5   STVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAAKKLF 64
           + V YN LI   C++   + A R+L DM  R   PN V+++ LI+ +   G +  A+KL+
Sbjct: 290 NVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNVVTFSALIDAFVKEGKLVEAEKLY 349

Query: 65  DEMPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKGFCHI 124
           DEM +   +P   TYS LI+  C+ ++  E  + F +M+  +C  +V+T NT+IKGFC  
Sbjct: 350 DEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFELMISKDCFPNVVTYNTLIKGFCKA 409

Query: 125 GSIEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVMREKGIM 181
             +++ +++  +M   +    + V +TT+I+G     R  ++  I++K M   G++
Sbjct: 410 KRVDEGMELFREM-SQRGLVGNTVTYTTLIHGFFQ-ARECDNAQIVFKQMVSDGVL 463

[Close]

BLAST of MS002153.1 vs. TAIR 10
Match: AT1G62590.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 100.1 bits (248), Expect = 1.9e-21
Identity = 63/179 (35.20%), Postives = 95/179 (53.07%), Query Frame = 0


Query: 1   EFVSSTVNYNRLIYQFCSFSLPNVAHRILFDMTGRGHCPNAVSYTTLINGYCCVGNVPAA 60
           E V     YN LI  FC  S  ++A  +L  M   G+ P+ V+ ++L+NGYC    +  A
Sbjct: 115 EIVHGLYTYNILINCFCRRSQISLALALLGKMMKLGYEPSIVTLSSLLNGYCHGKRISDA 174

Query: 61  KKLFDEMPENDAEPSSLTYSVLIHAICLANKSTELLNTFVVMLQTNCQSDVITLNTVIKG 120
             L D+M E    P ++T++ LIH + L NK++E +     M+Q  CQ +++T   V+ G
Sbjct: 175 VALVDQMVEMGYRPDTITFTTLIHGLFLHNKASEAVALVDRMVQRGCQPNLVTYGVVVNG 234

Query: 121 FCHIGSIEKALKVLNDMIICQFCAPDYVNFTTIIYGLLNVGRIQESLDILYKVMREKGI 180
            C  G  + AL +LN M   +  A D V F TII  L     + ++L+ L+K M  KGI
Sbjct: 235 LCKRGDTDLALNLLNKMEAAKIEA-DVVIFNTIIDSLCKYRHVDDALN-LFKEMETKGI 291

The following BLAST results are available for this feature:

BLAST of MS002153.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134613.1	5.0e-77	51.90	LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At3g18020 [Momo...	[more]
KAA0058133.1	8.6e-53	40.00	pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa]	[more]
XP_008453476.1	8.6e-53	40.00	PREDICTED: pentatricopeptide repeat-containing protein At3g18020 [Cucumis melo] ...	[more]
TYK28487.1	8.6e-53	40.00	pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa]	[more]
XP_011649150.1	1.2e-51	39.72	pentatricopeptide repeat-containing protein At3g18020 [Cucumis sativus] >KGN6387...	[more]

BLAST of MS002153.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSK8	2.5e-26	33.64	Pentatricopeptide repeat-containing protein At3g18020 OS=Arabidopsis thaliana OX...	[more]
Q9LUR2	1.5e-20	31.03	Putative pentatricopeptide repeat-containing protein At3g16710, mitochondrial OS...	[more]
Q9FMF6	1.5e-20	31.40	Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidop...	[more]
Q0WPZ6	2.6e-20	28.72	Pentatricopeptide repeat-containing protein At2g17140 OS=Arabidopsis thaliana OX...	[more]
Q9SXD8	2.6e-20	35.20	Pentatricopeptide repeat-containing protein At1g62590 OS=Arabidopsis thaliana OX...	[more]

BLAST of MS002153.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2H5	2.4e-77	51.90	LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At3g18020 OS=Mo...	[more]
A0A1S3BWE8	4.2e-53	40.00	pentatricopeptide repeat-containing protein At3g18020 OS=Cucumis melo OX=3656 GN...	[more]
A0A5D3DXA6	4.2e-53	40.00	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A5A7UQI7	4.2e-53	40.00	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0LV25	6.0e-52	39.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025030 PE=4 SV=1	[more]

BLAST of MS002153.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18020.1	1.7e-27	33.64	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT3G16710.1	1.1e-21	31.03	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT5G64320.1	1.1e-21	31.40	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT1G63130.1	1.9e-21	31.25	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT1G62590.1	1.9e-21	35.20	pentatricopeptide (PPR) repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002885	Pentatricopeptide repeat	TIGRFAM	TIGR00756	TIGR00756	coord: 42..75 e-value: 6.2E-9 score: 33.5 coord: 112..146 e-value: 2.8E-7 score: 28.3
IPR002885	Pentatricopeptide repeat	PFAM	PF12854	PPR_1	coord: 106..137 e-value: 6.7E-9 score: 35.3
IPR002885	Pentatricopeptide repeat	PFAM	PF13041	PPR_2	coord: 39..87 e-value: 1.3E-15 score: 57.3
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 110..144 score: 9.744654
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 40..74 score: 12.046526
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 146..180 score: 8.911594
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 95..180 e-value: 5.6E-16 score: 60.9
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 1..94 e-value: 1.5E-20 score: 75.3
None	No IPR available	PANTHER	PTHR47942:SF37	OS07G0674300 PROTEIN	coord: 97..179
None	No IPR available	PANTHER	PTHR47942:SF37	OS07G0674300 PROTEIN	coord: 1..90
None	No IPR available	PANTHER	PTHR47942	TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 97..179
None	No IPR available	PANTHER	PTHR47942	TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 1..90

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS002153	MS002153	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS002153.1-cds	MS002153.1-cds-scaffold30:2800493..2800779	CDS
MS002153.1-cds	MS002153.1-cds-scaffold30:2801269..2801521	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS002153.1	MS002153.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding