MS002029.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS002029.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like
Location	scaffold30: 1891506 .. 1893724 (+)
Sequence length	552
RNA-Seq Expression	MS002029.1
Synteny	MS002029.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAACATGGTGCATTTGCAAGGACGGAGTGAGTGATGCAGCTCTTCAGAAAACACTGGACTATGCGTGTGGAGCTGGGGCTGACTGTGTCCCTACTCATCAAAATGGGGGCTGTTTCCTTCCCAATACTGTCAAGGCACACTGTTCCTTCGCCACCAATAGCTACTTTCAGAGGAAGGGCCAAGCTCCAGGTTCCTGTGATTTCTCTGGCACAGCAACGATCACTACTACTGACCCCAGTAAGATTTAGCTGTCTACCTCCACATGTTTTTCTCTAGTTCCCTCCACTGTAAGAGAAGATTTAGTTAGTTGAGATCTGAATAATTTCGAGAGAGGAAAAATGGAAAAAAACCCTTCTCGGACTTAACTAGGATTCTAGTTATAGGCCAGAAATTCTAACCTTTTTGGCAATCTATCTGAGGTATCGAATCAAGGCTCCTTACCCATCTATTTCAGCTACCNTGTGGTAATCTTCAGTAATGAGTGAATGATATCTGATTTCCATTGTTATTTCTTGGTTTTTTTTTTCCTTCTTTTCAGGTGTTGCAGGTTGCGCTTATCCTTCCAGCGCTAGGTATGGTTCTGTATCTACCTTCACTATGTTTTTTTGAAACAAATAGAAAGGTATGAATAGTAAAATTTATTCCCATTTCTGTTTATGGCAATCGCTTTATTAGAATAAAGTTTTCTCAATTCTCAGATTCATTCCAAATCCAAGAATGAATTCTGAATCTGGAGGACAATGACATTCCCTCCAGAGTCAGTGAGATTGAAAATCTCATTCTTACATTCTCATTTCGTGAACTCCATCTCTTTGAAATGAAATAAAATTATGGTTAGTTTTATTCTTCCTCTTGTATCGACTTCAATCTCGTACATTAGTAAAAGGTTTACGATATTTTACTTCATTTGATGTTTTATTTTCATTTGAACAAACAGAGAGCCTTTTTACTTGAATAGGAGCAGAGCGTCTTGTTAATTAAAAACTCAAAACACAATTCCCATGATGGCCACCTACCTTAGGAATTAGTAATTCCTATGTGTTTCCTGGCACCAAATGTTGTGGGATCAGGCGGTTGCCACGTGAGATTAGTCGAGGTGTGCGTAAGCTGGTCCGGACACTTACAGATATCAAAAAAATAAAAATAAAAATTTAAAAATTAGAAACTAAACAACAAAAATCAAAGTTAACTCTCGATAATCATAGATACTGTAGGAAAAGAGACTTTTGCCATTAGATTCGCCATCATCCCATTGTGGTGTAATAATTGATACTCTTTTTGTCAATTGACTATTTTGTAATTTCATGACCATTTTTCTGGCTTCTTCGCTTACATTACCGTTTTTCAAACTTTCACTTTCACCCAAGAGGTTATAATTGTGACTCCCATGGTTATCTTTTAACACATAATTACCCCTTATCAGTAAGTAGACAAATTTTGTTAAAAAAAATTATGCCGGGCTGAAAATGGAGCCTTTCTTTGGTAATTTGCATCTGATTTATCATTTATGTTCCGTCATTGAGATGGGAAATTATCGAGATTTGAGCCAACATTTTAATGCCTCGAAAGGGAAGCCCAGACTGACCATAATTTTTGGAGAAAAGGATGAAGGTTTCTGAAGTAGTTTGTTTTCTTGAGGAAGAACTGTTGTCTTTTCTCTTGGAGCTAATTTGAAATGTTCTTGTCAAAAACTGGTTATTGTGTGAGGTTATTTTTACAGGTAGAAAGAATGAGATGAATCTTTAGATCCCTCATTGCTTATGCATGATCAACAAATGATGACAACTTTTCTTCTCTTCATAAAGAGATAGAAAATGAAAAGCTAGTTTATCCAAAACAAGGCCTTAACTTTATTAGTGAATCCAAACCCTTTTTCTAATGAGTTTTCTGTTTTCATAAAGTGGGATTCATTTCATTTTGGTAAATCTGTTGTTGCAGCCCTGTAAGCAGTGGCACCATTCCAGTAGGAGGAACTCCAACCACCACCAACCCAGGATTGACTCCCACAACCAACACTCCCATGGGTGGTTCGGCAACGCCCACCGGTGTCCTAGGCGGGAACGGGAACGGGTTCGGTGGCGGCAGCAACAGCCTAGGGCCATCGGGGGTCGGCGGGATGAGCACCGACTACAGCGACAGTGGATCAAGGCTCCAACTCACCCTCATTGGCAATGTAGCTATTTTTTTCTTCACTCTCTTGTGTTTTGGGCGTATGTTT

mRNA sequence

GCAACATGGTGCATTTGCAAGGACGGAGTGAGTGATGCAGCTCTTCAGAAAACACTGGACTATGCGTGTGGAGCTGGGGCTGACTGTGTCCCTACTCATCAAAATGGGGGCTGTTTCCTTCCCAATACTGTCAAGGCACACTGTTCCTTCGCCACCAATAGCTACTTTCAGAGGAAGGGCCAAGCTCCAGGTTCCTGTGATTTCTCTGGCACAGCAACGATCACTACTACTGACCCCAGTGTTGCAGGTTGCGCTTATCCTTCCAGCGCTAGCCCTGTAAGCAGTGGCACCATTCCAGTAGGAGGAACTCCAACCACCACCAACCCAGGATTGACTCCCACAACCAACACTCCCATGGGTGGTTCGGCAACGCCCACCGGTGTCCTAGGCGGGAACGGGAACGGGTTCGGTGGCGGCAGCAACAGCCTAGGGCCATCGGGGGTCGGCGGGATGAGCACCGACTACAGCGACAGTGGATCAAGGCTCCAACTCACCCTCATTGGCAATGTAGCTATTTTTTTCTTCACTCTCTTGTGTTTTGGGCGTATGTTT

Coding sequence (CDS)

GCAACATGGTGCATTTGCAAGGACGGAGTGAGTGATGCAGCTCTTCAGAAAACACTGGACTATGCGTGTGGAGCTGGGGCTGACTGTGTCCCTACTCATCAAAATGGGGGCTGTTTCCTTCCCAATACTGTCAAGGCACACTGTTCCTTCGCCACCAATAGCTACTTTCAGAGGAAGGGCCAAGCTCCAGGTTCCTGTGATTTCTCTGGCACAGCAACGATCACTACTACTGACCCCAGTGTTGCAGGTTGCGCTTATCCTTCCAGCGCTAGCCCTGTAAGCAGTGGCACCATTCCAGTAGGAGGAACTCCAACCACCACCAACCCAGGATTGACTCCCACAACCAACACTCCCATGGGTGGTTCGGCAACGCCCACCGGTGTCCTAGGCGGGAACGGGAACGGGTTCGGTGGCGGCAGCAACAGCCTAGGGCCATCGGGGGTCGGCGGGATGAGCACCGACTACAGCGACAGTGGATCAAGGCTCCAACTCACCCTCATTGGCAATGTAGCTATTTTTTTCTTCACTCTCTTGTGTTTTGGGCGTATGTTT

Protein sequence

ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKGQAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLLCFGRMF

Homology

[Close]

BLAST of MS002029.1 vs. NCBI nr
Match: XP_022135125.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Momordica charantia])

HSP 1 Score: 360.5 bits (924), Expect = 8.4e-96
Identity = 183/184 (99.46%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG
Sbjct: 23  ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 82

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG 120
           QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG
Sbjct: 83  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG 142

Query: 121 GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLLCF 180
           GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLLC 
Sbjct: 143 GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLLCV 202

Query: 181 GRMF 185
           GRMF
Sbjct: 203 GRMF 206

[Close]

BLAST of MS002029.1 vs. NCBI nr
Match: XP_038878558.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Benincasa hispida])

HSP 1 Score: 290.0 bits (741), Expect = 1.4e-74
Identity = 150/185 (81.08%), Postives = 162/185 (87.57%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+CKD V D ALQKTLD+ACGAGADC+P HQNGGCFLPNTVKAHCSFA NSYFQRKG
Sbjct: 28  ATWCVCKDEVGDTALQKTLDFACGAGADCIPIHQNGGCFLPNTVKAHCSFAANSYFQRKG 87

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP--GLTPTTNTP 120
           QAPGSCDFSGTA +TT+DPS++GC YPSSASPV+SGTIPV GTPTTTNP  GLTPTTNTP
Sbjct: 88  QAPGSCDFSGTAMVTTSDPSISGCVYPSSASPVNSGTIPVTGTPTTTNPGTGLTPTTNTP 147

Query: 121 MGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLL 180
             GS+TPTGVLG  GNGFG G+N LGPSGVGGM+TDYSDSGSRL+LTLI NV  F FTLL
Sbjct: 148 F-GSSTPTGVLG--GNGFGSGNNGLGPSGVGGMNTDYSDSGSRLELTLIANVLAFLFTLL 207

Query: 181 CFGRM 184
             G M
Sbjct: 208 LCGHM 209

[Close]

BLAST of MS002029.1 vs. NCBI nr
Match: XP_008453264.1 (PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo])

HSP 1 Score: 278.1 bits (710), Expect = 5.5e-71
Identity = 146/185 (78.92%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+CKDG++D  LQKTLD+ACGAGADC P HQNGGC+LPNTVKAHCSFATNSYFQRKG
Sbjct: 91  ATWCVCKDGLADTVLQKTLDFACGAGADCAPIHQNGGCYLPNTVKAHCSFATNSYFQRKG 150

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP--GLTPTTNTP 120
           QAPGSCDFSGTAT+T +DPS++GC YPSSASPV+SGTIPV GTPTTTNP  GLTPTTNTP
Sbjct: 151 QAPGSCDFSGTATVTGSDPSISGCVYPSSASPVNSGTIPVSGTPTTTNPGTGLTPTTNTP 210

Query: 121 MGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLL 180
             GS TPTGVLGGNG G  G +N LGPSGVGGM+TDYS S SRLQLTLI NV    FTLL
Sbjct: 211 F-GSTTPTGVLGGNGFG-SGNNNGLGPSGVGGMNTDYSGSRSRLQLTLIANVVGILFTLL 270

Query: 181 CFGRM 184
             G M
Sbjct: 271 LCGHM 273

[Close]

BLAST of MS002029.1 vs. NCBI nr
Match: KAA0057988.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo var. makuwa])

HSP 1 Score: 278.1 bits (710), Expect = 5.5e-71
Identity = 146/185 (78.92%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+CKDG++D  LQKTLD+ACGAGADC P HQNGGC+LPNTVKAHCSFATNSYFQRKG
Sbjct: 23  ATWCVCKDGLADTVLQKTLDFACGAGADCAPIHQNGGCYLPNTVKAHCSFATNSYFQRKG 82

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP--GLTPTTNTP 120
           QAPGSCDFSGTAT+T +DPS++GC YPSSASPV+SGTIPV GTPTTTNP  GLTPTTNTP
Sbjct: 83  QAPGSCDFSGTATVTGSDPSISGCVYPSSASPVNSGTIPVSGTPTTTNPGTGLTPTTNTP 142

Query: 121 MGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLL 180
             GS TPTGVLGGNG G  G +N LGPSGVGGM+TDYS S SRLQLTLI NV    FTLL
Sbjct: 143 F-GSTTPTGVLGGNGFG-SGNNNGLGPSGVGGMNTDYSGSRSRLQLTLIANVVGILFTLL 202

Query: 181 CFGRM 184
             G M
Sbjct: 203 LCGHM 205

[Close]

BLAST of MS002029.1 vs. NCBI nr
Match: XP_022933633.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Cucurbita moschata] >KAG7021544.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 276.6 bits (706), Expect = 1.6e-70
Identity = 145/187 (77.54%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+CK G+ DAALQKTLDYACGAGADCVP HQNGGCFLPNTV+AHCS+A NSYFQRKG
Sbjct: 23  ATWCVCKSGLGDAALQKTLDYACGAGADCVPIHQNGGCFLPNTVEAHCSYAANSYFQRKG 82

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGL----TPTTN 120
           QAPGSCDFSGTA +TTT+PS++GC YPSSASPV+SGTIPV GTPTTTNPG     T TTN
Sbjct: 83  QAPGSCDFSGTAMVTTTNPSISGCVYPSSASPVNSGTIPVSGTPTTTNPGTGFTPTTTTN 142

Query: 121 TPMGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFT 180
           TP  GS TPTG + G GNGFG GSN LGP GVGGM+TDYSDSGSRLQLTLI NV  F F 
Sbjct: 143 TPF-GSTTPTGGVMG-GNGFGTGSNGLGPPGVGGMNTDYSDSGSRLQLTLIANVLAFLFA 202

Query: 181 LLCFGRM 184
           L+ F  M
Sbjct: 203 LMFFNHM 207

[Close]

BLAST of MS002029.1 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.9e-34
Identity = 86/164 (52.44%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A+WC+CK G+SD  LQ TLDYACG GADC PT     CF P+ V++HC++A NS+FQ+KG
Sbjct: 19  ASWCVCKTGLSDTVLQATLDYACGNGADCNPTKPKQSCFNPDNVRSHCNYAVNSFFQKKG 78

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG 120
           Q+PGSC+F GTAT T +DPS  GCA+P+SAS  SSG+  V  TP TTNP  +PTT T + 
Sbjct: 79  QSPGSCNFDGTATPTNSDPSYTGCAFPTSASG-SSGSTTV--TPGTTNPKGSPTTTT-LP 138

Query: 121 GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQL 165
           GS T +   G   NG  GG+++ G +G  G++ DY+   S   L
Sbjct: 139 GSGTNSPYSGNPTNGVFGGNSTGGTTGT-GINPDYTTDSSAFAL 177

[Close]

BLAST of MS002029.1 vs. ExPASy Swiss-Prot
Match: Q9FZ86 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 5.6e-34
Identity = 86/174 (49.43%), Postives = 110/174 (63.22%), Query Frame = 0


Query: 2   TWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKGQ 61
           TWC+CK+G+S+A LQKTLDYACGAGADC P HQ G CF PNTVK+HCS+A NS+FQ+KGQ
Sbjct: 20  TWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVKSHCSYAVNSFFQKKGQ 79

Query: 62  APGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMGG 121
           + G+CDF+GTAT + +DPS   C +P+SAS   SGT     TP TT    TP+T  P   
Sbjct: 80  SLGTCDFAGTATFSASDPSYTTCPFPASAS--GSGTT----TPVTT----TPSTRVPTTT 139

Query: 122 SATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDS--GSRLQLTLIGNVAIF 174
           +  P  +    G G G  S         G++ DY+D   G +LQ    G + +F
Sbjct: 140 NTRPYTITPSTGGGLGIPS---------GINPDYTDPSFGFKLQSPRFGFIVLF 174

[Close]

BLAST of MS002029.1 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.8e-33
Identity = 92/183 (50.27%), Postives = 113/183 (61.75%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A+WC+CK G+SD+ LQKTLDYACG GADC PTH  G CF P+ V+AHC++A NS+FQ+KG
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACGNGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKKG 78

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP-GLTPTTNTPM 120
           QA  SC+F+GTAT+TTTDPS  GCA+PSSAS  SSG+     TP   +P G    T  P 
Sbjct: 79  QASESCNFTGTATLTTTDPSYTGCAFPSSASG-SSGSGSTTVTPGKNSPKGSNSITTFPG 138

Query: 121 GG---SATP-TGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFF 179
           G    S TP TG+LGGN     G           G++ DYS   S   L    N+ +  F
Sbjct: 139 GNSPYSGTPSTGLLGGNITDATG----------TGLNPDYSTESSGFALYYSNNLLLTGF 190

[Close]

BLAST of MS002029.1 vs. ExPASy Swiss-Prot
Match: Q93V72 (PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.8e-22
Identity = 82/201 (40.80%), Postives = 108/201 (53.73%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A +C+CK+G ++  LQK +DYACG GADC      G C+ PNTVK HC  A NSY+Q+K 
Sbjct: 19  AAYCLCKEG-NEQVLQKAIDYACGNGADCTQIQPTGACYQPNTVKNHCDVAVNSYYQKKA 78

Query: 61  QAPGSCDFSGTATITTTDPSVA-GCAYPSSASPV-SSGT----IPVGGTPTTTNPGL-TP 120
            +  +CDF+G A+ +TT PS A  C   SS+S   ++GT     P  GTPTT  P   TP
Sbjct: 79  SSGATCDFNGAASPSTTPPSTASNCLTGSSSSGTPTTGTPTTGTPTSGTPTTGTPTTGTP 138

Query: 121 TTNTPMGGSATP---------TGVLGG--NGNGFGGGSNS-------LGPSGVGGMSTDY 177
           TT TP  G+ T          TG   G  N NG    S+S       LGP+G GG+    
Sbjct: 139 TTGTPTSGTPTSGFPNTGTPNTGTNTGMPNSNGMPTSSSSSVFPGTTLGPTGSGGL---- 198

[Close]

BLAST of MS002029.1 vs. ExPASy Swiss-Prot
Match: Q9FZD0 (Carbohydrate-binding X8 domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=At1g26450 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.4e-16
Identity = 56/147 (38.10%), Postives = 78/147 (53.06%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A  C+CKD  ++  LQK +D+ACG GADC      G C+ PNT+K HC  A NSY+Q+K 
Sbjct: 19  AAVCVCKD-ANELDLQKVIDFACGGGADCAQIQTTGACYQPNTLKNHCDVAVNSYYQKKA 78

Query: 61  QAPGSCDFSGTATITTTDPS-VAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPM 120
               +CDF+G A I+T+ PS  + C   SS++   +   P  G  TT +PG T  +    
Sbjct: 79  STGATCDFNGAAVISTSPPSTTSSCLSSSSSNGTPTAGYPSTGNSTTASPGTTNPSTGNS 138

Query: 121 GGSATPTGVLGGNGNGFGGGSNSLGPS 147
             S  PT     +       S ++GPS
Sbjct: 139 TNSTLPTNDKPTSSTITFPDSTTMGPS 164

[Close]

BLAST of MS002029.1 vs. ExPASy TrEMBL
Match: A0A6J1C082 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Momordica charantia OX=3673 GN=LOC111007179 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.1e-96
Identity = 183/184 (99.46%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG
Sbjct: 23  ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 82

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG 120
           QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG
Sbjct: 83  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG 142

Query: 121 GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLLCF 180
           GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLLC 
Sbjct: 143 GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLLCV 202

Query: 181 GRMF 185
           GRMF
Sbjct: 203 GRMF 206

[Close]

BLAST of MS002029.1 vs. ExPASy TrEMBL
Match: A0A5A7US74 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002920 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.7e-71
Identity = 146/185 (78.92%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+CKDG++D  LQKTLD+ACGAGADC P HQNGGC+LPNTVKAHCSFATNSYFQRKG
Sbjct: 23  ATWCVCKDGLADTVLQKTLDFACGAGADCAPIHQNGGCYLPNTVKAHCSFATNSYFQRKG 82

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP--GLTPTTNTP 120
           QAPGSCDFSGTAT+T +DPS++GC YPSSASPV+SGTIPV GTPTTTNP  GLTPTTNTP
Sbjct: 83  QAPGSCDFSGTATVTGSDPSISGCVYPSSASPVNSGTIPVSGTPTTTNPGTGLTPTTNTP 142

Query: 121 MGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLL 180
             GS TPTGVLGGNG G  G +N LGPSGVGGM+TDYS S SRLQLTLI NV    FTLL
Sbjct: 143 F-GSTTPTGVLGGNGFG-SGNNNGLGPSGVGGMNTDYSGSRSRLQLTLIANVVGILFTLL 202

Query: 181 CFGRM 184
             G M
Sbjct: 203 LCGHM 205

[Close]

BLAST of MS002029.1 vs. ExPASy TrEMBL
Match: A0A1S3BVT1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103494037 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.7e-71
Identity = 146/185 (78.92%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+CKDG++D  LQKTLD+ACGAGADC P HQNGGC+LPNTVKAHCSFATNSYFQRKG
Sbjct: 91  ATWCVCKDGLADTVLQKTLDFACGAGADCAPIHQNGGCYLPNTVKAHCSFATNSYFQRKG 150

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP--GLTPTTNTP 120
           QAPGSCDFSGTAT+T +DPS++GC YPSSASPV+SGTIPV GTPTTTNP  GLTPTTNTP
Sbjct: 151 QAPGSCDFSGTATVTGSDPSISGCVYPSSASPVNSGTIPVSGTPTTTNPGTGLTPTTNTP 210

Query: 121 MGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLL 180
             GS TPTGVLGGNG G  G +N LGPSGVGGM+TDYS S SRLQLTLI NV    FTLL
Sbjct: 211 F-GSTTPTGVLGGNGFG-SGNNNGLGPSGVGGMNTDYSGSRSRLQLTLIANVVGILFTLL 270

Query: 181 CFGRM 184
             G M
Sbjct: 271 LCGHM 273

[Close]

BLAST of MS002029.1 vs. ExPASy TrEMBL
Match: A0A0A0LSF3 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G013210 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 7.7e-71
Identity = 147/185 (79.46%), Postives = 160/185 (86.49%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+C+DG+ D  LQKTLD+ACGAGADCVP HQNGGC+LPNTVKAHCSFATNSYFQRKG
Sbjct: 23  ATWCVCRDGLPDTVLQKTLDFACGAGADCVPIHQNGGCYLPNTVKAHCSFATNSYFQRKG 82

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP--GLTPTTNTP 120
           QAPGSCDFSGTA +TT+DPS++GCAYPSSASPV+SGTIPV GTPTTTNP  GLTPTTNTP
Sbjct: 83  QAPGSCDFSGTAAVTTSDPSISGCAYPSSASPVNSGTIPVTGTPTTTNPGNGLTPTTNTP 142

Query: 121 MGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLL 180
             GS TPTGVLGGNG G  G +N L PSGVGGM+TDY +SGSRLQLTLI NV  F FTLL
Sbjct: 143 F-GSTTPTGVLGGNGFG-SGNNNGLEPSGVGGMNTDY-NSGSRLQLTLIANVVGFLFTLL 202

Query: 181 CFGRM 184
             G M
Sbjct: 203 LCGHM 204

[Close]

BLAST of MS002029.1 vs. ExPASy TrEMBL
Match: A0A6J1F5D0 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LOC111440995 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 7.7e-71
Identity = 145/187 (77.54%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           ATWC+CK G+ DAALQKTLDYACGAGADCVP HQNGGCFLPNTV+AHCS+A NSYFQRKG
Sbjct: 23  ATWCVCKSGLGDAALQKTLDYACGAGADCVPIHQNGGCFLPNTVEAHCSYAANSYFQRKG 82

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGL----TPTTN 120
           QAPGSCDFSGTA +TTT+PS++GC YPSSASPV+SGTIPV GTPTTTNPG     T TTN
Sbjct: 83  QAPGSCDFSGTAMVTTTNPSISGCVYPSSASPVNSGTIPVSGTPTTTNPGTGFTPTTTTN 142

Query: 121 TPMGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFT 180
           TP  GS TPTG + G GNGFG GSN LGP GVGGM+TDYSDSGSRLQLTLI NV  F F 
Sbjct: 143 TPF-GSTTPTGGVMG-GNGFGTGSNGLGPPGVGGMNTDYSDSGSRLQLTLIANVLAFLFA 202

Query: 181 LLCFGRM 184
           L+ F  M
Sbjct: 203 LMFFNHM 207

[Close]

BLAST of MS002029.1 vs. TAIR 10
Match: AT5G61130.1 (plasmodesmata callose-binding protein 1 )

HSP 1 Score: 147.1 bits (370), Expect = 1.4e-35
Identity = 86/164 (52.44%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A+WC+CK G+SD  LQ TLDYACG GADC PT     CF P+ V++HC++A NS+FQ+KG
Sbjct: 19  ASWCVCKTGLSDTVLQATLDYACGNGADCNPTKPKQSCFNPDNVRSHCNYAVNSFFQKKG 78

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMG 120
           Q+PGSC+F GTAT T +DPS  GCA+P+SAS  SSG+  V  TP TTNP  +PTT T + 
Sbjct: 79  QSPGSCNFDGTATPTNSDPSYTGCAFPTSASG-SSGSTTV--TPGTTNPKGSPTTTT-LP 138

Query: 121 GSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQL 165
           GS T +   G   NG  GG+++ G +G  G++ DY+   S   L
Sbjct: 139 GSGTNSPYSGNPTNGVFGGNSTGGTTGT-GINPDYTTDSSAFAL 177

[Close]

BLAST of MS002029.1 vs. TAIR 10
Match: AT1G18650.1 (plasmodesmata callose-binding protein 3 )

HSP 1 Score: 145.6 bits (366), Expect = 4.0e-35
Identity = 86/174 (49.43%), Postives = 110/174 (63.22%), Query Frame = 0


Query: 2   TWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKGQ 61
           TWC+CK+G+S+A LQKTLDYACGAGADC P HQ G CF PNTVK+HCS+A NS+FQ+KGQ
Sbjct: 20  TWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVKSHCSYAVNSFFQKKGQ 79

Query: 62  APGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNPGLTPTTNTPMGG 121
           + G+CDF+GTAT + +DPS   C +P+SAS   SGT     TP TT    TP+T  P   
Sbjct: 80  SLGTCDFAGTATFSASDPSYTTCPFPASAS--GSGTT----TPVTT----TPSTRVPTTT 139

Query: 122 SATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDS--GSRLQLTLIGNVAIF 174
           +  P  +    G G G  S         G++ DY+D   G +LQ    G + +F
Sbjct: 140 NTRPYTITPSTGGGLGIPS---------GINPDYTDPSFGFKLQSPRFGFIVLF 174

[Close]

BLAST of MS002029.1 vs. TAIR 10
Match: AT5G08000.1 (glucan endo-1,3-beta-glucosidase-like protein 3 )

HSP 1 Score: 143.3 bits (360), Expect = 2.0e-34
Identity = 92/183 (50.27%), Postives = 113/183 (61.75%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A+WC+CK G+SD+ LQKTLDYACG GADC PTH  G CF P+ V+AHC++A NS+FQ+KG
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACGNGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKKG 78

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPVSSGTIPVGGTPTTTNP-GLTPTTNTPM 120
           QA  SC+F+GTAT+TTTDPS  GCA+PSSAS  SSG+     TP   +P G    T  P 
Sbjct: 79  QASESCNFTGTATLTTTDPSYTGCAFPSSASG-SSGSGSTTVTPGKNSPKGSNSITTFPG 138

Query: 121 GG---SATP-TGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFF 179
           G    S TP TG+LGGN     G           G++ DYS   S   L    N+ +  F
Sbjct: 139 GNSPYSGTPSTGLLGGNITDATG----------TGLNPDYSTESSGFALYYSNNLLLTGF 190

[Close]

BLAST of MS002029.1 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-30
Identity = 84/182 (46.15%), Postives = 104/182 (57.14%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A WC+ +  V+  ALQ  LDYAC AGADC P   NG CFLPNTV+AH S+A NSYFQR  
Sbjct: 59  AMWCVARFDVTSQALQAALDYACAAGADCAPIQPNGLCFLPNTVQAHASYAFNSYFQRAA 118

Query: 61  QAPGSCDFSGTATITTTDPSVAGCAYPSSASPV--SSGTIPVGGTPTTTNPGLTPTTNTP 120
            APGSC+F+GT+TI  TDPS   C YP+S      S+ T  VGGTP+TT      TT  P
Sbjct: 119 MAPGSCNFAGTSTIAKTDPSYGSCVYPNSVRNAGGSASTTTVGGTPSTTVGNSPMTTLRP 178

Query: 121 MGGSATPTGVLGGNGNGFGGGSNSLGPSGVGGMSTDYSDSGSRLQLTLIGNVAIFFFTLL 180
             G+ T         + FG G   L P G    +T+  +SGS +      ++A+F   LL
Sbjct: 179 PSGTTT---------SPFGIGGGGLNPQGT-TTTTNTDESGSYIINKSTVSIALFVALLL 230

[Close]

BLAST of MS002029.1 vs. TAIR 10
Match: AT1G13830.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-27
Identity = 79/159 (49.69%), Postives = 94/159 (59.12%), Query Frame = 0


Query: 1   ATWCICKDGVSDAALQKTLDYACGAGADCVPTHQNGGCFLPNTVKAHCSFATNSYFQRKG 60
           A +C+CKDG+ D  LQ ++DYACG  ADC P H  G C+ P+T+K+HC +A NSYFQ   
Sbjct: 19  AIYCLCKDGIGDTELQTSIDYACGTLADCNPIHDKGTCYQPDTIKSHCDWAVNSYFQNAA 78

Query: 61  QAPGSCDFSGTATITTTDPS--VAGCAYPSSASPV---SSGTIPVGGTP---TTTNPGLT 120
           Q PGSC+FSGTAT     PS    GC YPSS S      S T P G TP   TT  PG T
Sbjct: 79  QVPGSCNFSGTATTNPNPPSNLANGCIYPSSPSSTRSPPSTTPPTGTTPTNGTTPFPG-T 138

Query: 121 PTTNTPMGGSAT---PTGVLGGNGNGFGGGSNSLGPSGV 149
           P   TP  G+     PTGV   N +  G G++SLG S V
Sbjct: 139 PFPGTPFPGTPPVFGPTGVF--NPSNPGSGASSLGTSSV 174

The following BLAST results are available for this feature:

BLAST of MS002029.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135125.1	8.4e-96	99.46	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Momordica charantia]	[more]
XP_038878558.1	1.4e-74	81.08	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Benincasa hispida]	[more]
XP_008453264.1	5.5e-71	78.92	PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo]	[more]
KAA0057988.1	5.5e-71	78.92	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo var. makuwa]	[more]
XP_022933633.1	1.6e-70	77.54	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Cucurbita moschata] >KAG7021544.1 ...	[more]

BLAST of MS002029.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FNQ2	1.9e-34	52.44	PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1...	[more]
Q9FZ86	5.6e-34	49.43	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3...	[more]
Q9SD84	2.8e-33	50.27	PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2...	[more]
Q93V72	3.8e-22	40.80	PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4...	[more]
Q9FZD0	1.4e-16	38.10	Carbohydrate-binding X8 domain-containing protein OS=Arabidopsis thaliana OX=370...	[more]

BLAST of MS002029.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C082	4.1e-96	99.46	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A5A7US74	2.7e-71	78.92	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BVT1	2.7e-71	78.92	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103494037 ...	[more]
A0A0A0LSF3	7.7e-71	79.46	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G013210 PE=4 SV=...	[more]
A0A6J1F5D0	7.7e-71	77.54	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of MS002029.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61130.1	1.4e-35	52.44	plasmodesmata callose-binding protein 1	[more]
AT1G18650.1	4.0e-35	49.43	plasmodesmata callose-binding protein 3	[more]
AT5G08000.1	2.0e-34	50.27	glucan endo-1,3-beta-glucosidase-like protein 3	[more]
AT4G13600.1	1.0e-30	46.15	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G13830.1	1.0e-27	49.69	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 2..86 e-value: 5.1E-45 score: 165.6
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 2..73 e-value: 1.8E-20 score: 73.4
None	No IPR available	GENE3D	1.20.58.1040		coord: 1..94 e-value: 1.5E-9 score: 40.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 93..115
None	No IPR available	PANTHER	PTHR31044:SF25	PLASMODESMATA CALLOSE-BINDING PROTEIN 3	coord: 1..166
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 1..166

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS002029	MS002029	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS002029.1-cds	MS002029.1-cds-scaffold30:1891506..1891743	CDS
MS002029.1-cds	MS002029.1-cds-scaffold30:1892045..1892078	CDS
MS002029.1-cds	MS002029.1-cds-scaffold30:1893445..1893724	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS002029.1	MS002029.1-protein	polypeptide