MS002025.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS002025.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein Asterix
Location	scaffold30: 1863586 .. 1863921 (-)
Sequence length	243
RNA-Seq Expression	MS002025.1
Synteny	MS002025.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGACACAAGCTAACGATCCACGGCAGCCGTCTGCGGCGAAGCCTTACGTTGCTCCGGCGGTGAGCCCGCAAGATCTGCCGGTCGATTATTCCGGTTTCATCGCCGTAGTTTTCGGCATCGCCGGTGTCATGTTCAGGGTATTTCTATTTCTTTTTGAGCACTGGTACGGTAACTATGTCGATCTCACTTTTCACTCACTCGGACTCTTTGTTTTGGTTTAATTTGAGCAGTACAAGCTTAGTTCGTGGCTGGCCATCATATTTTGCGCCCAATCGTTAGTGAATATGAGGAACATGGAGAACGATCTTAAGCAGATTTCCATGGCCATGATG

mRNA sequence

ATGTCGACACAAGCTAACGATCCACGGCAGCCGTCTGCGGCGAAGCCTTACGTTGCTCCGGCGGTGAGCCCGCAAGATCTGCCGGTCGATTATTCCGGTTTCATCGCCGTAGTTTTCGGCATCGCCGGTGTCATGTTCAGGTACAAGCTTAGTTCGTGGCTGGCCATCATATTTTGCGCCCAATCGTTAGTGAATATGAGGAACATGGAGAACGATCTTAAGCAGATTTCCATGGCCATGATG

Coding sequence (CDS)

ATGTCGACACAAGCTAACGATCCACGGCAGCCGTCTGCGGCGAAGCCTTACGTTGCTCCGGCGGTGAGCCCGCAAGATCTGCCGGTCGATTATTCCGGTTTCATCGCCGTAGTTTTCGGCATCGCCGGTGTCATGTTCAGGTACAAGCTTAGTTCGTGGCTGGCCATCATATTTTGCGCCCAATCGTTAGTGAATATGAGGAACATGGAGAACGATCTTAAGCAGATTTCCATGGCCATGATG

Protein sequence

MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCAQSLVNMRNMENDLKQISMAMM

Homology

[Close]

BLAST of MS002025.1 vs. NCBI nr
Match: XP_022134591.1 (protein Asterix [Momordica charantia])

HSP 1 Score: 157.5 bits (397), Expect = 4.8e-35
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
          MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60

Query: 61 QSLVNMRNMENDLKQISMAMM 82
          QSLVNMRNMENDLKQISMAMM
Sbjct: 61 QSLVNMRNMENDLKQISMAMM 81

[Close]

BLAST of MS002025.1 vs. NCBI nr
Match: XP_022933634.1 (protein Asterix-like [Cucurbita moschata] >XP_023007397.1 protein Asterix-like [Cucurbita maxima] >XP_023529496.1 protein Asterix-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 152.1 bits (383), Expect = 2.0e-33
Identity = 77/81 (95.06%), Postives = 80/81 (98.77%), Query Frame = 0


Query: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
          MSTQANDPRQPSAAKP+V+PAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 1  MSTQANDPRQPSAAKPFVSPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60

Query: 61 QSLVNMRNMENDLKQISMAMM 82
          QSL NMRNMENDLKQISMAMM
Sbjct: 61 QSLANMRNMENDLKQISMAMM 81

[Close]

BLAST of MS002025.1 vs. NCBI nr
Match: KAG6587575.1 (Protein Asterix, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 152.1 bits (383), Expect = 2.0e-33
Identity = 77/81 (95.06%), Postives = 80/81 (98.77%), Query Frame = 0


Query: 1   MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
           MSTQANDPRQPSAAKP+V+PAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 47  MSTQANDPRQPSAAKPFVSPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 106

Query: 61  QSLVNMRNMENDLKQISMAMM 82
           QSL NMRNMENDLKQISMAMM
Sbjct: 107 QSLANMRNMENDLKQISMAMM 127

[Close]

BLAST of MS002025.1 vs. NCBI nr
Match: KAG7021543.1 (Protein Asterix, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 152.1 bits (383), Expect = 2.0e-33
Identity = 77/81 (95.06%), Postives = 80/81 (98.77%), Query Frame = 0


Query: 1   MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
           MSTQANDPRQPSAAKP+V+PAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 47  MSTQANDPRQPSAAKPFVSPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 106

Query: 61  QSLVNMRNMENDLKQISMAMM 82
           QSL NMRNMENDLKQISMAMM
Sbjct: 107 QSLANMRNMENDLKQISMAMM 127

[Close]

BLAST of MS002025.1 vs. NCBI nr
Match: XP_038880376.1 (protein Asterix [Benincasa hispida])

HSP 1 Score: 151.8 bits (382), Expect = 2.6e-33
Identity = 77/81 (95.06%), Postives = 80/81 (98.77%), Query Frame = 0


Query: 1   MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
           MSTQANDPRQPSAAKP+VAPAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 30  MSTQANDPRQPSAAKPFVAPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 89

Query: 61  QSLVNMRNMENDLKQISMAMM 82
           QSL NMRN+ENDLKQISMAMM
Sbjct: 90  QSLANMRNVENDLKQISMAMM 110

[Close]

BLAST of MS002025.1 vs. ExPASy Swiss-Prot
Match: Q9SD88 (Protein Asterix OS=Arabidopsis thaliana OX=3702 GN=At5g07960 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.4e-29
Identity = 62/80 (77.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 2  STQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCAQ 61
          ++  NDPRQPSAAKPY+   V+P+DLPVDYSGFIAV+ G++GVMFRYK+ SWLAIIFCAQ
Sbjct: 8  ASSVNDPRQPSAAKPYIPRPVAPEDLPVDYSGFIAVILGVSGVMFRYKICSWLAIIFCAQ 67

Query: 62 SLVNMRNMENDLKQISMAMM 82
          SL NMRN+ENDLKQISMAMM
Sbjct: 68 SLANMRNLENDLKQISMAMM 87

[Close]

BLAST of MS002025.1 vs. ExPASy TrEMBL
Match: A0A6J1BY81 (protein Asterix OS=Momordica charantia OX=3673 GN=LOC111006820 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.3e-35
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
          MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60

Query: 61 QSLVNMRNMENDLKQISMAMM 82
          QSLVNMRNMENDLKQISMAMM
Sbjct: 61 QSLVNMRNMENDLKQISMAMM 81

[Close]

BLAST of MS002025.1 vs. ExPASy TrEMBL
Match: A0A6J1KYK4 (protein Asterix-like OS=Cucurbita maxima OX=3661 GN=LOC111499903 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.7e-34
Identity = 77/81 (95.06%), Postives = 80/81 (98.77%), Query Frame = 0


Query: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
          MSTQANDPRQPSAAKP+V+PAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 1  MSTQANDPRQPSAAKPFVSPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60

Query: 61 QSLVNMRNMENDLKQISMAMM 82
          QSL NMRNMENDLKQISMAMM
Sbjct: 61 QSLANMRNMENDLKQISMAMM 81

[Close]

BLAST of MS002025.1 vs. ExPASy TrEMBL
Match: A0A6J1F0A5 (protein Asterix-like OS=Cucurbita moschata OX=3662 GN=LOC111440996 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.7e-34
Identity = 77/81 (95.06%), Postives = 80/81 (98.77%), Query Frame = 0


Query: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
          MSTQANDPRQPSAAKP+V+PAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 1  MSTQANDPRQPSAAKPFVSPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60

Query: 61 QSLVNMRNMENDLKQISMAMM 82
          QSL NMRNMENDLKQISMAMM
Sbjct: 61 QSLANMRNMENDLKQISMAMM 81

[Close]

BLAST of MS002025.1 vs. ExPASy TrEMBL
Match: A0A5A7UWJ6 (Protein Asterix OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002890 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.7e-33
Identity = 76/81 (93.83%), Postives = 79/81 (97.53%), Query Frame = 0


Query: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
          MSTQANDPRQPSA KP+VAPAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 1  MSTQANDPRQPSAVKPFVAPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60

Query: 61 QSLVNMRNMENDLKQISMAMM 82
          QSL NMRN+ENDLKQISMAMM
Sbjct: 61 QSLANMRNVENDLKQISMAMM 81

[Close]

BLAST of MS002025.1 vs. ExPASy TrEMBL
Match: A0A0A0LSF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G013160 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.7e-33
Identity = 76/81 (93.83%), Postives = 79/81 (97.53%), Query Frame = 0


Query: 1  MSTQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60
          MSTQANDPRQPSA KP+VAPAV+PQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA
Sbjct: 1  MSTQANDPRQPSAVKPFVAPAVNPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCA 60

Query: 61 QSLVNMRNMENDLKQISMAMM 82
          QSL NMRN+ENDLKQISMAMM
Sbjct: 61 QSLANMRNIENDLKQISMAMM 81

[Close]

BLAST of MS002025.1 vs. TAIR 10
Match: AT5G07960.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0139 (InterPro:IPR005351); Has 193 Blast hits to 193 proteins in 75 species: Archae - 0; Bacteria - 0; Metazoa - 130; Fungi - 0; Plants - 52; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 129.8 bits (325), Expect = 9.9e-31
Identity = 62/80 (77.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 2  STQANDPRQPSAAKPYVAPAVSPQDLPVDYSGFIAVVFGIAGVMFRYKLSSWLAIIFCAQ 61
          ++  NDPRQPSAAKPY+   V+P+DLPVDYSGFIAV+ G++GVMFRYK+ SWLAIIFCAQ
Sbjct: 8  ASSVNDPRQPSAAKPYIPRPVAPEDLPVDYSGFIAVILGVSGVMFRYKICSWLAIIFCAQ 67

Query: 62 SLVNMRNMENDLKQISMAMM 82
          SL NMRN+ENDLKQISMAMM
Sbjct: 68 SLANMRNLENDLKQISMAMM 87

The following BLAST results are available for this feature:

BLAST of MS002025.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134591.1	4.8e-35	100.00	protein Asterix [Momordica charantia]	[more]
XP_022933634.1	2.0e-33	95.06	protein Asterix-like [Cucurbita moschata] >XP_023007397.1 protein Asterix-like [...	[more]
KAG6587575.1	2.0e-33	95.06	Protein Asterix, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7021543.1	2.0e-33	95.06	Protein Asterix, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038880376.1	2.6e-33	95.06	protein Asterix [Benincasa hispida]	[more]

BLAST of MS002025.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SD88	1.4e-29	77.50	Protein Asterix OS=Arabidopsis thaliana OX=3702 GN=At5g07960 PE=3 SV=1	[more]

BLAST of MS002025.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BY81	2.3e-35	100.00	protein Asterix OS=Momordica charantia OX=3673 GN=LOC111006820 PE=4 SV=1	[more]
A0A6J1KYK4	9.7e-34	95.06	protein Asterix-like OS=Cucurbita maxima OX=3661 GN=LOC111499903 PE=4 SV=1	[more]
A0A6J1F0A5	9.7e-34	95.06	protein Asterix-like OS=Cucurbita moschata OX=3662 GN=LOC111440996 PE=4 SV=1	[more]
A0A5A7UWJ6	3.7e-33	93.83	Protein Asterix OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G0028...	[more]
A0A0A0LSF0	3.7e-33	93.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G013160 PE=4 SV=1	[more]

BLAST of MS002025.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07960.1	9.9e-31	77.50	unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005351	PAT complex subunit Asterix	PFAM	PF03669	UPF0139	coord: 5..81 e-value: 3.8E-27 score: 94.0
IPR005351	PAT complex subunit Asterix	PANTHER	PTHR13193	CGI-140	coord: 2..81

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS002025	MS002025	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS002025.1-cds	MS002025.1-cds-scaffold30:1863586..1863687	CDS
MS002025.1-cds	MS002025.1-cds-scaffold30:1863781..1863921	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS002025.1	MS002025.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045048	protein insertion into ER membrane
cellular_component	GO:0030176	integral component of endoplasmic reticulum membrane
molecular_function	GO:0044183	protein folding chaperone