MS001855.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS001855.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	EF hand calcium-binding family protein
Location	scaffold30: 408207 .. 408608 (-)
Sequence length	402
RNA-Seq Expression	MS001855.1
Synteny	MS001855.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGTTCGAGCGTGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGCTCGGGCTGATGGGCGGAGAGCTGTTGCCGGCGGAGGCAGAGCTGGCGGTGGAATCGCTGGACTCCGACGGAGACGGATTGCTGTGCGTCGGCGATCTCGCACGGCTACTGGACGTCGGAGAGGACGAGAAATTAAAGGATCTGAAAGAGGCTTTCGCGATGTACGATGCGGAAGGCTGCGGATTCATAACTCCCAAGGGATTGAAGAGAATGCTCAGAAAATTGGGGGAACGGAAATCCACGAAGGAGTGCAAAGCCATGATTTGTCGCTTCGATTTGAACGGCGACGGTCTGATTAGCTTCGAGGAGTTTCAGATCATGATGGCC

mRNA sequence

AAGTTCGAGCGTGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGCTCGGGCTGATGGGCGGAGAGCTGTTGCCGGCGGAGGCAGAGCTGGCGGTGGAATCGCTGGACTCCGACGGAGACGGATTGCTGTGCGTCGGCGATCTCGCACGGCTACTGGACGTCGGAGAGGACGAGAAATTAAAGGATCTGAAAGAGGCTTTCGCGATGTACGATGCGGAAGGCTGCGGATTCATAACTCCCAAGGGATTGAAGAGAATGCTCAGAAAATTGGGGGAACGGAAATCCACGAAGGAGTGCAAAGCCATGATTTGTCGCTTCGATTTGAACGGCGACGGTCTGATTAGCTTCGAGGAGTTTCAGATCATGATGGCC

Coding sequence (CDS)

AAGTTCGAGCGTGTATTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGCTCGGGCTGATGGGCGGAGAGCTGTTGCCGGCGGAGGCAGAGCTGGCGGTGGAATCGCTGGACTCCGACGGAGACGGATTGCTGTGCGTCGGCGATCTCGCACGGCTACTGGACGTCGGAGAGGACGAGAAATTAAAGGATCTGAAAGAGGCTTTCGCGATGTACGATGCGGAAGGCTGCGGATTCATAACTCCCAAGGGATTGAAGAGAATGCTCAGAAAATTGGGGGAACGGAAATCCACGAAGGAGTGCAAAGCCATGATTTGTCGCTTCGATTTGAACGGCGACGGTCTGATTAGCTTCGAGGAGTTTCAGATCATGATGGCC

Protein sequence

KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLARLLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNGDGLISFEEFQIMMA

Homology

[Close]

BLAST of MS001855.1 vs. NCBI nr
Match: XP_022134910.1 (putative calcium-binding protein CML19 [Momordica charantia])

HSP 1 Score: 271.2 bits (692), Expect = 4.9e-69
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR
Sbjct: 5   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 64

Query: 61  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 120
           LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG
Sbjct: 65  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 124

Query: 121 DGLISFEEFQIMMA 135
           DGLISFEEFQIMMA
Sbjct: 125 DGLISFEEFQIMMA 138

[Close]

BLAST of MS001855.1 vs. NCBI nr
Match: XP_038879778.1 (putative calcium-binding protein CML19 [Benincasa hispida])

HSP 1 Score: 240.0 bits (611), Expect = 1.2e-59
Identity = 117/134 (87.31%), Postives = 125/134 (93.28%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           +++RVLSYFDEDGDGKISPSELRNRLGLMGGELL AEAE AVESLDSDGDGLLC GD  R
Sbjct: 39  QYDRVLSYFDEDGDGKISPSELRNRLGLMGGELLQAEAEAAVESLDSDGDGLLCAGDFER 98

Query: 61  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 120
           LL+VGE+EKLKDLKEAFA+YD EGCGFITPK LK+MLRKLGERKST+ECK MI RFDLNG
Sbjct: 99  LLEVGEEEKLKDLKEAFALYDCEGCGFITPKNLKKMLRKLGERKSTEECKLMIRRFDLNG 158

Query: 121 DGLISFEEFQIMMA 135
           DGLISFEEFQIMMA
Sbjct: 159 DGLISFEEFQIMMA 172

[Close]

BLAST of MS001855.1 vs. NCBI nr
Match: KAG7021401.1 (putative calcium-binding protein CML31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 240.0 bits (611), Expect = 1.2e-59
Identity = 116/133 (87.22%), Postives = 125/133 (93.98%), Query Frame = 0


Query: 2   FERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLARL 61
           ++RVL YFDEDGDGKISPSELRNRLGL+GGELLPAEAE+AVESLDSDGDGLLCVGD+ RL
Sbjct: 9   YDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEVAVESLDSDGDGLLCVGDIERL 68

Query: 62  LDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNGD 121
           LDVGE+EKLKDL+EAF +YDAEGCGFITPK LKRML+KLGE KST ECK MI RFDLNGD
Sbjct: 69  LDVGEEEKLKDLREAFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDLNGD 128

Query: 122 GLISFEEFQIMMA 135
           GLISFEEFQIMMA
Sbjct: 129 GLISFEEFQIMMA 141

[Close]

BLAST of MS001855.1 vs. NCBI nr
Match: XP_008464450.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0057778.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa] >TYJ98459.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa])

HSP 1 Score: 239.6 bits (610), Expect = 1.6e-59
Identity = 116/134 (86.57%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           +++RVLSYFDEDGDGKISPSELRNRLGL+GGELL AEAE AVESLDSDGDGLLC GD+ R
Sbjct: 9   QYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGLLCAGDIER 68

Query: 61  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 120
           LL+VGE+EKLKDLKEAFA+YD+EGCGFITPK LK+MLRKLGERKST+ECK MI RFDLNG
Sbjct: 69  LLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRRFDLNG 128

Query: 121 DGLISFEEFQIMMA 135
           DGLISFEEFQIMMA
Sbjct: 129 DGLISFEEFQIMMA 142

[Close]

BLAST of MS001855.1 vs. NCBI nr
Match: XP_022925940.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 239.2 bits (609), Expect = 2.1e-59
Identity = 116/133 (87.22%), Postives = 124/133 (93.23%), Query Frame = 0


Query: 2   FERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLARL 61
           ++RVL YFDEDGDGKISPSELRNRLGL+GGELLPAEAE AVESLDSDGDGLLCVGD+ RL
Sbjct: 9   YDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEAAVESLDSDGDGLLCVGDIERL 68

Query: 62  LDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNGD 121
           LDVGE+EKLKDL+EAF +YDAEGCGFITPK LKRML+KLGE KST ECK MI RFDLNGD
Sbjct: 69  LDVGEEEKLKDLREAFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDLNGD 128

Query: 122 GLISFEEFQIMMA 135
           GLISFEEFQIMMA
Sbjct: 129 GLISFEEFQIMMA 141

[Close]

BLAST of MS001855.1 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 3.6e-30
Identity = 73/138 (52.90%), Postives = 94/138 (68.12%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRL-GLMGGELLPAEAELAVESLDSDGDGLLCVGDLA 60
           +F RV S FD D DGKIS +ELR  +   +G ++   EAE  V S D+D DGLL   +  
Sbjct: 7   EFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEFT 66

Query: 61  RL---LDVG-EDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICR 120
           +L   L++G E+E+ + L EAF MY+ EG G ITP  LKRML KLG  +  +EC+ MICR
Sbjct: 67  KLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMICR 126

Query: 121 FDLNGDGLISFEEFQIMM 134
           FDL+GDG+ISFEEF+IMM
Sbjct: 127 FDLDGDGVISFEEFKIMM 144

[Close]

BLAST of MS001855.1 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.3e-29
Identity = 68/131 (51.91%), Postives = 90/131 (68.70%), Query Frame = 0


Query: 9   FDEDGDGKISPSELRNRL-GLMGGELLPAEAELAVESLDSDGDGLLCVGDLARLLDVGED 68
           FD DGDG+IS +ELR  +   +G E+   EA   V S+D+DGDGLLC  +  RL+   E 
Sbjct: 19  FDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQAAEV 78

Query: 69  EKLKD-----LKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNGDG 128
           E+  +     L+EAF MY+ EG G ITP  L+RMLR+LG  +   +C+AMICRFDLNGDG
Sbjct: 79  EEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDLNGDG 138

Query: 129 LISFEEFQIMM 134
           ++SF+EF+IMM
Sbjct: 139 VLSFDEFKIMM 149

[Close]

BLAST of MS001855.1 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 5.7e-28
Identity = 70/144 (48.61%), Postives = 92/144 (63.89%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESL-DSDGDGLLCVGDLA 60
           +F RV   FD+DGDGKIS +ELR  +    GE +P E   A+ +L D+DGDGLL   +  
Sbjct: 6   EFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFV 65

Query: 61  RLL----------DVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKEC 120
           RL+          +  +DE  + L+EAFAMY+ EG G ITP  LK ML KLG      EC
Sbjct: 66  RLVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAEC 125

Query: 121 KAMICRFDLNGDGLISFEEFQIMM 134
           +AMICRFD+NGDG+++F+EF+ MM
Sbjct: 126 QAMICRFDMNGDGVLTFDEFKTMM 149

[Close]

BLAST of MS001855.1 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-27
Identity = 64/134 (47.76%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           + E V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   + ++
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 61  LLDV-GEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLN 120
           L+ V  E+EK  +LK AF +Y AEG   ITP+ LK ML+KLGE ++T +C+ MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 121 GDGLISFEEFQIMM 134
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]

BLAST of MS001855.1 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.3e-26
Identity = 62/135 (45.93%), Postives = 86/135 (63.70%), Query Frame = 0


Query: 3   ERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLARLL 62
           E V +Y D + DG+IS  EL+     +G ++   EAE AV+  D DGDG+L + + A L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 63  ----DVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDL 122
               +  E+EK + + EAF MY A+G   ITP  LK ML KLGE ++T +CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 123 NGDGLISFEEFQIMM 134
           N DG++SF+EF +MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of MS001855.1 vs. ExPASy TrEMBL
Match: A0A6J1BZN1 (putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC111007048 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.4e-69
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR
Sbjct: 5   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 64

Query: 61  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 120
           LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG
Sbjct: 65  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 124

Query: 121 DGLISFEEFQIMMA 135
           DGLISFEEFQIMMA
Sbjct: 125 DGLISFEEFQIMMA 138

[Close]

BLAST of MS001855.1 vs. ExPASy TrEMBL
Match: A0A5A7URJ2 (Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00610 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.6e-60
Identity = 116/134 (86.57%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           +++RVLSYFDEDGDGKISPSELRNRLGL+GGELL AEAE AVESLDSDGDGLLC GD+ R
Sbjct: 9   QYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGLLCAGDIER 68

Query: 61  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 120
           LL+VGE+EKLKDLKEAFA+YD+EGCGFITPK LK+MLRKLGERKST+ECK MI RFDLNG
Sbjct: 69  LLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRRFDLNG 128

Query: 121 DGLISFEEFQIMMA 135
           DGLISFEEFQIMMA
Sbjct: 129 DGLISFEEFQIMMA 142

[Close]

BLAST of MS001855.1 vs. ExPASy TrEMBL
Match: A0A1S3CN12 (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.6e-60
Identity = 116/134 (86.57%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           +++RVLSYFDEDGDGKISPSELRNRLGL+GGELL AEAE AVESLDSDGDGLLC GD+ R
Sbjct: 9   QYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGLLCAGDIER 68

Query: 61  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 120
           LL+VGE+EKLKDLKEAFA+YD+EGCGFITPK LK+MLRKLGERKST+ECK MI RFDLNG
Sbjct: 69  LLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRRFDLNG 128

Query: 121 DGLISFEEFQIMMA 135
           DGLISFEEFQIMMA
Sbjct: 129 DGLISFEEFQIMMA 142

[Close]

BLAST of MS001855.1 vs. ExPASy TrEMBL
Match: A0A6J1EJM6 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111433200 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.0e-59
Identity = 116/133 (87.22%), Postives = 124/133 (93.23%), Query Frame = 0


Query: 2   FERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLARL 61
           ++RVL YFDEDGDGKISPSELRNRLGL+GGELLPAEAE AVESLDSDGDGLLCVGD+ RL
Sbjct: 9   YDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEAAVESLDSDGDGLLCVGDIERL 68

Query: 62  LDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNGD 121
           LDVGE+EKLKDL+EAF +YDAEGCGFITPK LKRML+KLGE KST ECK MI RFDLNGD
Sbjct: 69  LDVGEEEKLKDLREAFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMIRRFDLNGD 128

Query: 122 GLISFEEFQIMMA 135
           GLISFEEFQIMMA
Sbjct: 129 GLISFEEFQIMMA 141

[Close]

BLAST of MS001855.1 vs. ExPASy TrEMBL
Match: A0A0A0LU40 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.3e-59
Identity = 116/134 (86.57%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           +++RVLSYFDEDGDGKISPSELRNRLGL+GGEL  AEAE AVESLDSDGDGLLCVGD+ R
Sbjct: 9   QYDRVLSYFDEDGDGKISPSELRNRLGLIGGELQQAEAEAAVESLDSDGDGLLCVGDIER 68

Query: 61  LLDVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNG 120
           LL+VGE+EKLKDLKEAFA+YD+EGCGFITPK LK+MLRKLGERKST+ECK MI RFDLNG
Sbjct: 69  LLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMMIRRFDLNG 128

Query: 121 DGLISFEEFQIMMA 135
           DGLISFEEFQIMMA
Sbjct: 129 DGLISFEEFQIMMA 142

[Close]

BLAST of MS001855.1 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 124.0 bits (310), Expect = 9.0e-29
Identity = 64/134 (47.76%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           + E V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   + ++
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 61  LLDV-GEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLN 120
           L+ V  E+EK  +LK AF +Y AEG   ITP+ LK ML+KLGE ++T +C+ MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 121 GDGLISFEEFQIMM 134
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]

BLAST of MS001855.1 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 124.0 bits (310), Expect = 9.0e-29
Identity = 64/134 (47.76%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           + E V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   + ++
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 61  LLDV-GEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLN 120
           L+ V  E+EK  +LK AF +Y AEG   ITP+ LK ML+KLGE ++T +C+ MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 121 GDGLISFEEFQIMM 134
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 176

[Close]

BLAST of MS001855.1 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 118.6 bits (296), Expect = 3.8e-27
Identity = 62/135 (45.93%), Postives = 86/135 (63.70%), Query Frame = 0


Query: 3   ERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLARLL 62
           E V +Y D + DG+IS  EL+     +G ++   EAE AV+  D DGDG+L + + A L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 63  ----DVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDL 122
               +  E+EK + + EAF MY A+G   ITP  LK ML KLGE ++T +CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 123 NGDGLISFEEFQIMM 134
           N DG++SF+EF +MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of MS001855.1 vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 114.8 bits (286), Expect = 5.5e-26
Identity = 61/134 (45.52%), Postives = 86/134 (64.18%), Query Frame = 0


Query: 1   KFERVLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLAR 60
           + E V SY D + DG+ISP EL+     +G +LL           D+DGDG+L   + ++
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLLS----------DTDGDGMLDFEEFSQ 102

Query: 61  LLDV-GEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLN 120
           L+ V  E+EK  +LK AF +Y AEG   ITP+ LK ML+KLGE ++T +C+ MI  FDLN
Sbjct: 103 LIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 162

Query: 121 GDGLISFEEFQIMM 134
            DG++SF+EF +MM
Sbjct: 163 ADGVLSFDEFALMM 166

[Close]

BLAST of MS001855.1 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 110.2 bits (274), Expect = 1.3e-24
Identity = 59/132 (44.70%), Postives = 81/132 (61.36%), Query Frame = 0


Query: 5   VLSYFDEDGDGKISPSELRNRLGLMGGELLPAEAELAVESLDSDGDGLLCVGDLARLL-- 64
           V  Y D + DGKIS  EL++ + L+GG L   E E  V++ D DGDG +   +  +L+  
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 65  -DVGEDEKLKDLKEAFAMYDAEGCGFITPKGLKRMLRKLGERKSTKECKAMICRFDLNGD 124
            D  ++E+ K+LKEAF MY  EG  FIT   L+R L +LGE  +   CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 125 GLISFEEFQIMM 134
           G++SF+EF +MM
Sbjct: 173 GVLSFDEFVLMM 184

The following BLAST results are available for this feature:

BLAST of MS001855.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134910.1	4.9e-69	100.00	putative calcium-binding protein CML19 [Momordica charantia]	[more]
XP_038879778.1	1.2e-59	87.31	putative calcium-binding protein CML19 [Benincasa hispida]	[more]
KAG7021401.1	1.2e-59	87.22	putative calcium-binding protein CML31, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_008464450.1	1.6e-59	86.57	PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0057778.1 p...	[more]
XP_022925940.1	2.1e-59	87.22	putative calcium-binding protein CML19 [Cucurbita moschata]	[more]

BLAST of MS001855.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYJ8	3.6e-30	52.90	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYK0	2.3e-29	51.91	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYJ9	5.7e-28	48.61	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE6	1.3e-27	47.76	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q9SRE7	5.3e-26	45.93	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]

BLAST of MS001855.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZN1	2.4e-69	100.00	putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5A7URJ2	7.6e-60	86.57	Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CN12	7.6e-60	86.57	putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103502330 P...	[more]
A0A6J1EJM6	1.0e-59	87.22	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A0A0LU40	1.3e-59	86.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002110 PE=4 SV=1	[more]

BLAST of MS001855.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76650.1	9.0e-29	47.76	calmodulin-like 38	[more]
AT1G76650.3	9.0e-29	47.76	calmodulin-like 38	[more]
AT1G76640.1	3.8e-27	45.93	Calcium-binding EF-hand family protein	[more]
AT1G76650.2	5.5e-26	45.52	calmodulin-like 38	[more]
AT5G42380.1	1.3e-24	44.70	calmodulin like 37	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 108..134 e-value: 0.23 score: 20.6 coord: 1..29 e-value: 0.033 score: 23.3 coord: 72..100 e-value: 0.1 score: 21.7
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 5..24 e-value: 1.4E-5 score: 24.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 71..133 e-value: 6.4E-13 score: 49.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 104..134 score: 13.927603
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 68..103 score: 11.612284
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 1..32 score: 12.281773
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 72..134 e-value: 9.81673E-16 score: 64.8765
None	No IPR available	GENE3D	1.10.238.10		coord: 61..134 e-value: 3.7E-22 score: 80.4
None	No IPR available	GENE3D	1.10.238.10		coord: 1..60 e-value: 5.5E-10 score: 41.4
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..134
None	No IPR available	PANTHER	PTHR10891:SF870	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 2..134
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 117..129
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 10..22
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 2..133

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS001855	MS001855	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS001855.1-cds	MS001855.1-cds-scaffold30:408207..408608	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS001855.1	MS001855.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding