MS001365.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS001365.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMajor facilitator superfamily protein
Locationscaffold36: 3581626 .. 3584177 (-)
Sequence length1509
RNA-Seq ExpressionMS001365.1
SyntenyMS001365.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGAGGAAGAGAAGCTTAAAGCCTCTGCTGAAGCTTTGGAAGAAGAAGATGAGCCCCAGAAAGAGTCTGGTGGCTGTATTGGCACGGCACTATATTGGTTCGAAATTCTCGTCAGGGAAATGCATTGGAGTTTTGTGTTAGGGATAGTGATTGTTTATGGAATAAGTCAGGGATTAGGTGGAGCGCTTAACCGTGTTGCGACCGAGTACTACATGAAGGATGTTCAGAAAGTGCAGCCATCTGAAGCACAGGTTTATTCAGGAATCACCTCAATTCCATGGATGGTTAAGCCTATCTGGGGTCTTCTCACTGATTTAGTCCCCATTCTTGGGTACCAAAGGCGACCTTACTTTGTTTTAGCAGGTAAAATCGTTCGCTGCGTCCTGTCGACGCCGAATTTGTCTGGTTCAATAGTGTGCTGAATATGAAGCTCAAAGTAAACAAATTTGGATATGTTTTTTTAGAGTGTTTTGGAATTTTCCAACTTTTGGATCTTTGAGGCTCTTAACAGTGTGAACTTTTCATGATTGAGTAAGTTTCTATGTTATCCTACCTAGATCTTCACTTTGGTTAAGAAAGTTCATATTAGATCTCTTCAATCAATTGCCAAAGTAAATAGCCTGTAGAGGTTCTATTATTGAGATTCTTTTCTTTTGAATGCTTTGGATAGGTTGCTTATTCTTACAAATTTTCAGTGCTATTAGGAGTAAAAATTTCCAGTGTTCTATTTTTAGACTGCTTTGGATGAGCTTGAGATGAATGCATGAGTTTCATCTATTTTAGTAATAACCAATTTTGAAAAGATTCTTATATTATGTTGGACTTGAGTTATATTTCAGGATTTTCATAACCAAGTTATTTTCTTGGGTAAGGCATCCCTTTTGGAATGTCCTGTAACTAGAGCCATTAGTTACATCTAAAGGTGCACACATATGTTCAATGACGAACCCCCATATCGTTTATAAGTCGATCTGATGGCGAAATGTCTGTTAACTTCCCTTCATGTTTCTTCTCTATTCCAGGTCTTCTTGGTGTGATCTCCTTGCTTTCATTAGCATTACACGAGAAGCTACATCTTCTACTCGCAATTTTGTTGTTAACGGCAGGAAGCGCCGGGGTAGCCGTAGCCGATGTCACCATAGATGCGTGTGTAGCACAGAACAGTAGCATTCATCCTACTCTTGCAGCTGATCTGCAGAGCTTGTGCGCCTTGAGTTCTTCAGTCGGAGCATTACTTGGATTCTCTATTAGCGGTATCCTTGTTCACCTAATAGGCTCCAAGGTTCTCTACTATTTAAACTCTTTGCTGCTTCATACTTGTAATTTCTATTCTTCATTCTTTCATATGCTTGGGAGATTTACTTCCTTTTCCTCTCTCTGATAGGGAGTTTACGGTCTGTTAGCAATACCCGCCGGGCTTGTTTTATTGGTTGGAATTCTACTCAATGAACCCCATCTGCCAGATTTCAACTACAGACAGGTATTCTACAGCATCTGTTATGTGTAATGCCTTCCTTTGGACTGATTCTTACTTCATCTTTGGGAATCAGGTAAACGAAAAGTTTGTTGGCGCTGGGAAGGCTATGTGGGCGACGTTAAAGTGCCCGAATGTCTGGAGGCCATGCCTCTATATGTATCTATCTCTAACATTGTGCCTTGACATTAATGAGGGACTGTTTTATTGGTACACAGACTCAAAAGATGGTCCAAATTTCTCTCAGGTAGATTTCCATCATTGAACTTCTTCTTCGTATACCGAGACGTAATGTAGTATCTAGGATATCGACATCTCGGGCCCGAACCTTGGTTATATAAATCAACTTTTGAATAGACATTAGGTCTGAGAAAGGTTGGTCAAAATCATTCTAACTTTTAGGAGGCTGAAGTTAAGCTAGTATCTGTTTTACTTCATCTTTAAACTCCTTTCTTGCAGGAAAATGTTGGTTTCATTTTCTCAATTGGTTCAGTTGGGTCTCTTTTAGGAGCTTTACTGTATCAATATGTTCTCAAGGATCACCAATTCCGAGACTTGCTATTCTGGACACAATTGATATTCAGTTTATCTGGGATGCTCGATTTCTTGCTGGTTTTACGTTTAAACTTGAAATTTGGAGTGCCAGATTATTTCTTCATTGTGATAGATGAGAGTGTACATCAGCTTATCAATAGGCTAAAATGGATGCCTCTTCTAGTCCTCAGCTCCAAGCTCTGCCCCAGAGGCATTGAGGGGACATTCTTTGCTCTGCTTATGTCGATCGACAATGTCGGACTTCTCTCAGCGTCATGGGGAGGCGGTTTCCTGCTCCATATCCTTAAAGTTACTCGAACCAAGTTCGATAACCTATGGCTAGCCATTTTGATAAGGAACATCTTGAGACTTACTCCACTTTGTATGCTGTTTTTGGTACCGAGAGGCGATCCGAATTCTTCAATTCTTCTAACCGAGCTGCCCAGCGAAGAAGTCGACCACGAGACTTGTGAAGCCGATAACATTGAACTGGTTTCCCTTGTCAATGGCATCAACATAGACAACCAGAAAGAAGTTCTT

mRNA sequence

ATGGTGGAGGAAGAGAAGCTTAAAGCCTCTGCTGAAGCTTTGGAAGAAGAAGATGAGCCCCAGAAAGAGTCTGGTGGCTGTATTGGCACGGCACTATATTGGTTCGAAATTCTCGTCAGGGAAATGCATTGGAGTTTTGTGTTAGGGATAGTGATTGTTTATGGAATAAGTCAGGGATTAGGTGGAGCGCTTAACCGTGTTGCGACCGAGTACTACATGAAGGATGTTCAGAAAGTGCAGCCATCTGAAGCACAGGTTTATTCAGGAATCACCTCAATTCCATGGATGGTTAAGCCTATCTGGGGTCTTCTCACTGATTTAGTCCCCATTCTTGGGTACCAAAGGCGACCTTACTTTGTTTTAGCAGGTCTTCTTGGTGTGATCTCCTTGCTTTCATTAGCATTACACGAGAAGCTACATCTTCTACTCGCAATTTTGTTGTTAACGGCAGGAAGCGCCGGGGTAGCCGTAGCCGATGTCACCATAGATGCGTGTGTAGCACAGAACAGTAGCATTCATCCTACTCTTGCAGCTGATCTGCAGAGCTTGTGCGCCTTGAGTTCTTCAGTCGGAGCATTACTTGGATTCTCTATTAGCGGTATCCTTGTTCACCTAATAGGCTCCAAGGGAGTTTACGGTCTGTTAGCAATACCCGCCGGGCTTGTTTTATTGGTTGGAATTCTACTCAATGAACCCCATCTGCCAGATTTCAACTACAGACAGGTAAACGAAAAGTTTGTTGGCGCTGGGAAGGCTATGTGGGCGACGTTAAAGTGCCCGAATGTCTGGAGGCCATGCCTCTATATGTATCTATCTCTAACATTGTGCCTTGACATTAATGAGGGACTGTTTTATTGGTACACAGACTCAAAAGATGGTCCAAATTTCTCTCAGGAAAATGTTGGTTTCATTTTCTCAATTGGTTCAGTTGGGTCTCTTTTAGGAGCTTTACTGTATCAATATGTTCTCAAGGATCACCAATTCCGAGACTTGCTATTCTGGACACAATTGATATTCAGTTTATCTGGGATGCTCGATTTCTTGCTGGTTTTACGTTTAAACTTGAAATTTGGAGTGCCAGATTATTTCTTCATTGTGATAGATGAGAGTGTACATCAGCTTATCAATAGGCTAAAATGGATGCCTCTTCTAGTCCTCAGCTCCAAGCTCTGCCCCAGAGGCATTGAGGGGACATTCTTTGCTCTGCTTATGTCGATCGACAATGTCGGACTTCTCTCAGCGTCATGGGGAGGCGGTTTCCTGCTCCATATCCTTAAAGTTACTCGAACCAAGTTCGATAACCTATGGCTAGCCATTTTGATAAGGAACATCTTGAGACTTACTCCACTTTGTATGCTGTTTTTGGTACCGAGAGGCGATCCGAATTCTTCAATTCTTCTAACCGAGCTGCCCAGCGAAGAAGTCGACCACGAGACTTGTGAAGCCGATAACATTGAACTGGTTTCCCTTGTCAATGGCATCAACATAGACAACCAGAAAGAAGTTCTT

Coding sequence (CDS)

ATGGTGGAGGAAGAGAAGCTTAAAGCCTCTGCTGAAGCTTTGGAAGAAGAAGATGAGCCCCAGAAAGAGTCTGGTGGCTGTATTGGCACGGCACTATATTGGTTCGAAATTCTCGTCAGGGAAATGCATTGGAGTTTTGTGTTAGGGATAGTGATTGTTTATGGAATAAGTCAGGGATTAGGTGGAGCGCTTAACCGTGTTGCGACCGAGTACTACATGAAGGATGTTCAGAAAGTGCAGCCATCTGAAGCACAGGTTTATTCAGGAATCACCTCAATTCCATGGATGGTTAAGCCTATCTGGGGTCTTCTCACTGATTTAGTCCCCATTCTTGGGTACCAAAGGCGACCTTACTTTGTTTTAGCAGGTCTTCTTGGTGTGATCTCCTTGCTTTCATTAGCATTACACGAGAAGCTACATCTTCTACTCGCAATTTTGTTGTTAACGGCAGGAAGCGCCGGGGTAGCCGTAGCCGATGTCACCATAGATGCGTGTGTAGCACAGAACAGTAGCATTCATCCTACTCTTGCAGCTGATCTGCAGAGCTTGTGCGCCTTGAGTTCTTCAGTCGGAGCATTACTTGGATTCTCTATTAGCGGTATCCTTGTTCACCTAATAGGCTCCAAGGGAGTTTACGGTCTGTTAGCAATACCCGCCGGGCTTGTTTTATTGGTTGGAATTCTACTCAATGAACCCCATCTGCCAGATTTCAACTACAGACAGGTAAACGAAAAGTTTGTTGGCGCTGGGAAGGCTATGTGGGCGACGTTAAAGTGCCCGAATGTCTGGAGGCCATGCCTCTATATGTATCTATCTCTAACATTGTGCCTTGACATTAATGAGGGACTGTTTTATTGGTACACAGACTCAAAAGATGGTCCAAATTTCTCTCAGGAAAATGTTGGTTTCATTTTCTCAATTGGTTCAGTTGGGTCTCTTTTAGGAGCTTTACTGTATCAATATGTTCTCAAGGATCACCAATTCCGAGACTTGCTATTCTGGACACAATTGATATTCAGTTTATCTGGGATGCTCGATTTCTTGCTGGTTTTACGTTTAAACTTGAAATTTGGAGTGCCAGATTATTTCTTCATTGTGATAGATGAGAGTGTACATCAGCTTATCAATAGGCTAAAATGGATGCCTCTTCTAGTCCTCAGCTCCAAGCTCTGCCCCAGAGGCATTGAGGGGACATTCTTTGCTCTGCTTATGTCGATCGACAATGTCGGACTTCTCTCAGCGTCATGGGGAGGCGGTTTCCTGCTCCATATCCTTAAAGTTACTCGAACCAAGTTCGATAACCTATGGCTAGCCATTTTGATAAGGAACATCTTGAGACTTACTCCACTTTGTATGCTGTTTTTGGTACCGAGAGGCGATCCGAATTCTTCAATTCTTCTAACCGAGCTGCCCAGCGAAGAAGTCGACCACGAGACTTGTGAAGCCGATAACATTGAACTGGTTTCCCTTGTCAATGGCATCAACATAGACAACCAGAAAGAAGTTCTT

Protein sequence

MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETCEADNIELVSLVNGINIDNQKEVL
Homology
BLAST of MS001365.1 vs. NCBI nr
Match: XP_022132109.1 (probable folate-biopterin transporter 2 [Momordica charantia])

HSP 1 Score: 976.5 bits (2523), Expect = 8.9e-281
Identity = 501/503 (99.60%), Postives = 502/503 (99.80%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL
Sbjct: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
           LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL
Sbjct: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR
Sbjct: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSL LCLDINEGLFYWYTDSKDGPNFSQEN
Sbjct: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPNFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP
Sbjct: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC
Sbjct: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480

Query: 481 EADNIELVSLVNGINIDNQKEVL 504
           EADNIELVSLVNG+NIDNQKEVL
Sbjct: 481 EADNIELVSLVNGMNIDNQKEVL 503

BLAST of MS001365.1 vs. NCBI nr
Match: XP_038885031.1 (probable folate-biopterin transporter 2 [Benincasa hispida])

HSP 1 Score: 896.3 bits (2315), Expect = 1.2e-256
Identity = 462/503 (91.85%), Postives = 478/503 (95.03%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEEEKLK S E L EED+PQKESG  IG ALYWF++LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEEKLKVSVEDL-EEDDPQKESGVSIGRALYWFKLLVSEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRV+T+YYMKDVQKVQPSEAQVYSGITSIPWMVKP+WGLLTDLVPILGY RRPYFV
Sbjct: 61  GGALNRVSTKYYMKDVQKVQPSEAQVYSGITSIPWMVKPVWGLLTDLVPILGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALHEKLHLLLAILLLTAGSA VA+ADVTIDACVAQNS+IHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHEKLHLLLAILLLTAGSASVAIADVTIDACVAQNSNIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIP GLV LVGILLNEPH+PDFNYR
Sbjct: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPGGLVFLVGILLNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW TLK PNVWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWTTLKIPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSLLGALLYQYV KDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFG+P
Sbjct: 301 VGFIFSIGSVGSLLGALLYQYVFKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGIP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQL+NRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKF NLWLAILIRN+LRL PL MLFLVPRGDPNSSILLTELPS EVD+ET 
Sbjct: 421 LLHILKVTRTKFANLWLAILIRNLLRLIPLFMLFLVPRGDPNSSILLTELPSSEVDNETS 480

Query: 481 -EADNIELVSLVNGINIDNQKEV 503
            EADNIELVSLVNG+NIDNQKEV
Sbjct: 481 EEADNIELVSLVNGMNIDNQKEV 502

BLAST of MS001365.1 vs. NCBI nr
Match: KAG7029472.1 (putative folate-biopterin transporter 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.0 bits (2309), Expect = 5.8e-256
Identity = 458/502 (91.24%), Postives = 476/502 (94.82%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEE+KL    E L EEDEPQKES   IGTA+YWF+ LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEDKLNLFDEDL-EEDEPQKESSVSIGTAVYWFKTLVSEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGL TDLVP+LGY RRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPVLGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALH+KLHLLLAILLLTAGSAGVA+ADVTIDACVAQNSSIHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHQKLHLLLAILLLTAGSAGVAIADVTIDACVAQNSSIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSS+GALLGFSISGILVHLIGSKGVYGLLAIPAGLV LVG++LNEPH+PDFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPAGLVFLVGMILNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW TLK PNVWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWTTLKSPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSL GALLYQYVLKDHQFRDLLFWTQ+I+SLSGMLDFLLVLRLNLK G+P
Sbjct: 301 VGFIFSIGSVGSLFGALLYQYVLKDHQFRDLLFWTQMIYSLSGMLDFLLVLRLNLKIGMP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKF NLWLAILIRN LRLTPLCMLFLVPRGDPNSSILLTELPS +V++ETC
Sbjct: 421 LLHILKVTRTKFSNLWLAILIRNFLRLTPLCMLFLVPRGDPNSSILLTELPSSDVENETC 480

Query: 481 -EADNIELVSLVNGINIDNQKE 502
            EADNIELVSLVNGINIDNQKE
Sbjct: 481 EEADNIELVSLVNGINIDNQKE 501

BLAST of MS001365.1 vs. NCBI nr
Match: XP_023547193.1 (probable folate-biopterin transporter 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 893.3 bits (2307), Expect = 9.9e-256
Identity = 458/502 (91.24%), Postives = 475/502 (94.62%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEE+KL    E L EEDEPQKES   IGTA+YWF+ LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEDKLNVFDEDL-EEDEPQKESSVSIGTAVYWFKTLVSEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGL TDLVP+LGY RRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPVLGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALH+KLHLLLAILLLTAGSAGVA+ADVTIDACVAQNSSIHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHQKLHLLLAILLLTAGSAGVAIADVTIDACVAQNSSIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSS+GALLGFSISGILVHLIGSKGVYGLLAIPAGLV LVG++LNEPH+PDFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPAGLVFLVGMILNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW TLK PNVWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWTTLKSPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSL GALLYQYVLKDHQFRDLLFWTQ+I+SLSGMLDFLLVLRLNLK G+P
Sbjct: 301 VGFIFSIGSVGSLFGALLYQYVLKDHQFRDLLFWTQMIYSLSGMLDFLLVLRLNLKIGMP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKF NLWLAILIRN LRLTPLCMLFLVPRGDPNSSILLTELPS +V +ETC
Sbjct: 421 LLHILKVTRTKFSNLWLAILIRNFLRLTPLCMLFLVPRGDPNSSILLTELPSSDVGNETC 480

Query: 481 -EADNIELVSLVNGINIDNQKE 502
            EADNIELVSLVNGINIDNQKE
Sbjct: 481 EEADNIELVSLVNGINIDNQKE 501

BLAST of MS001365.1 vs. NCBI nr
Match: XP_022962138.1 (probable folate-biopterin transporter 2 [Cucurbita moschata])

HSP 1 Score: 890.6 bits (2300), Expect = 6.4e-255
Identity = 457/502 (91.04%), Postives = 475/502 (94.62%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEE+KL    E L EEDEPQKES   IGTA+YWF+ LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEDKLNVFDEDL-EEDEPQKESSVSIGTAVYWFKTLVSEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGL TDLVP+LGY RRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPVLGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALH+KLHLLLAILLLTAGSAGVA+ADVTIDACVAQNSSIHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHQKLHLLLAILLLTAGSAGVAIADVTIDACVAQNSSIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSS+GALLGFSISGILVHLIGSKGVYGLLAIPAGLV LVG++LNEPH+PDFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPAGLVFLVGMILNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW TLK PNVWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWTTLKSPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSL GALLYQYVLKDHQFRDLLFWTQ+I+SLSGMLDFLLVLRLNLK G+P
Sbjct: 301 VGFIFSIGSVGSLFGALLYQYVLKDHQFRDLLFWTQMIYSLSGMLDFLLVLRLNLKIGMP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKF NLWLAILIRN LRLTPLCMLFLVPRGDPNSSILLTEL S +V++ETC
Sbjct: 421 LLHILKVTRTKFSNLWLAILIRNFLRLTPLCMLFLVPRGDPNSSILLTELLSSDVENETC 480

Query: 481 -EADNIELVSLVNGINIDNQKE 502
            EADNIELVSLVNGINIDNQKE
Sbjct: 481 EEADNIELVSLVNGINIDNQKE 501

BLAST of MS001365.1 vs. ExPASy Swiss-Prot
Match: Q5FV41 (Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g25050 PE=2 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 3.4e-182
Identity = 317/498 (63.65%), Postives = 398/498 (79.92%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           +VEE+ L+        + E    +  C    + W ++L  E+HWSFV G+V +YGI+QGL
Sbjct: 2   VVEEQSLENGGSVHVVKGESNFRNVVC--GPVRWLKMLSSELHWSFVFGVVSLYGINQGL 61

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GG+L RVATEYYMKDVQKVQPSE+Q  + IT IPW++KP+WG+LTD++PI G+ RRPYF+
Sbjct: 62  GGSLGRVATEYYMKDVQKVQPSESQALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFI 121

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
           LAG+LGV+SLL ++LH  LHL LA+  +T  SA +A+ADVTIDAC A NS  HP+LA+D+
Sbjct: 122 LAGVLGVVSLLFISLHSNLHLYLALFWMTISSAAMAIADVTIDACTAYNSIKHPSLASDM 181

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLC+LSSS+GALLGF +SGILVHL+GSKGV+GLL  P  LV +VGI+ +EPH+P F+Y+
Sbjct: 182 QSLCSLSSSIGALLGFFMSGILVHLVGSKGVFGLLTFPFALVSVVGIVFSEPHVPGFSYK 241

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVN+KF  AGKAMW T+KC +VWRP LYMY+SLTL L+I+EGLFYW+TDSKDGP F+QE 
Sbjct: 242 QVNQKFTDAGKAMWRTMKCSDVWRPSLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQET 301

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFI SIGS+GS+L A LYQ VLKDH FR L  WTQL+F+LSGMLD +LV RLNLKFG+P
Sbjct: 302 VGFILSIGSIGSILAATLYQLVLKDHPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLP 361

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DY FIV+DE V Q+I RLKWMPLLVL+SKLCP GIEGTFFALLMSIDN GL+++SW GG 
Sbjct: 362 DYLFIVVDEIVSQMIGRLKWMPLLVLTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGI 421

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           +LH+LKVTRT+F NLWLA+L+RN++RL PLC LFLVP+GD N+  L  E+  E+ D E  
Sbjct: 422 MLHVLKVTRTEFGNLWLAVLVRNVMRLLPLCFLFLVPKGDQNTFKLPDEIMGEDSDEEKD 481

Query: 481 EAD---NIELVSLVNGIN 496
           E +   N+EL SLV+ ++
Sbjct: 482 EKEGTRNLELASLVHSVD 497

BLAST of MS001365.1 vs. ExPASy Swiss-Prot
Match: Q9SQN2 (Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana OX=3702 GN=At1g79710 PE=2 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 8.6e-162
Identity = 275/461 (59.65%), Postives = 365/461 (79.18%), Query Frame = 0

Query: 32  LYWFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGIT 91
           L W  +L+ E+HWSF  G++IVYG+SQGLG  L++V+T+YY KD QK+QPS+AQ+Y G+ 
Sbjct: 35  LRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQYYFKDEQKIQPSQAQIYVGLI 94

Query: 92  SIPWMVKPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAG 151
            IPW++KP+WGLLTD+VP+LGY+RRPYF+LAG L +IS++ L LH  LHL LA+  L AG
Sbjct: 95  QIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISMMVLWLHTNLHLALALSCLVAG 154

Query: 152 SAGVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGV 211
           SAGVA+ADVTIDACV Q S  HPTLAAD+QSLC L SS+G+L+GFS+SG+LVHL+GSKGV
Sbjct: 155 SAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSIGSLVGFSLSGVLVHLVGSKGV 214

Query: 212 YGLLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAGKAMWATLKCPNVWRPCLYMYL 271
           YGLL + AGL+++VG++L E        + VN+KF+ AG A+W T +   VWRPCL+M L
Sbjct: 215 YGLLGVTAGLLVVVGMVLKESPSRSLGRKHVNDKFLDAGSAIWKTFQYGEVWRPCLFMLL 274

Query: 272 SLTLCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDL 331
           S  + L I+EG+FYWYTDSKDGP+FS+E VG I S G++GSL+G LLYQ  LK+  FR++
Sbjct: 275 SAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIMSFGAIGSLVGILLYQNFLKNFPFRNV 334

Query: 332 LFWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKLC 391
           +FW   +  LSG LD +LVLR+NLK G+PDYFFIV+DE V  +I+R+KW+PLLVLSSKLC
Sbjct: 335 VFWALSLSVLSGFLDLILVLRINLKLGLPDYFFIVVDEFVSHMISRIKWLPLLVLSSKLC 394

Query: 392 PRGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKVTRTKFDNLWLAILIRNILRLTPLC 451
           P G+EGTFFALLMSI+++G L +SWGGG LLH LKVTRT+FDNLWL I+IR++LR+ P+ 
Sbjct: 395 PAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHALKVTRTQFDNLWLVIVIRSLLRVIPIG 454

Query: 452 MLFLVPRGDPNSSILLTELPSEEVDHETCEADNIELVSLVN 493
           ++FL+P  D NS+IL  E+ +  +  E  + + IE+ +L++
Sbjct: 455 LVFLIPNVDSNSTILPAEMLTSRIS-EGVDTEKIEMTALIS 494

BLAST of MS001365.1 vs. ExPASy Swiss-Prot
Match: F4KIL8 (Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana OX=3702 GN=At5g25040 PE=2 SV=2)

HSP 1 Score: 549.3 bits (1414), Expect = 4.6e-155
Identity = 273/463 (58.96%), Postives = 357/463 (77.11%), Query Frame = 0

Query: 34  WFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGITSI 93
           W ++L  ++HWS+V  +V   GI+QG G +L  VAT+YYMKDVQKVQPS+ Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAGS 153
            W++ KP++G+LTD++PI G+ RRPYF+LAG++GV+SLL ++L   LHL LA+  +T  S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGVY 213
           A +A+ADVTIDAC   NS+ HP+LA+D+QSLC+LS S+G LLGF +SGILVHL+GSKGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAGKAMWATLKCPNVWRPCLYMYLS 273
           GLL     LV +VG+L +EP +  F+++Q    F  A KAMW T+KC +VW+P LYM+++
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQ---NFTNAMKAMWRTIKCSDVWQPSLYMFIT 272

Query: 274 LTLCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLL 333
             L LDI EGLFYW+TDSKDGP F+QE VGFI SIGS+GS+LG LLY   LKDH FR L 
Sbjct: 273 RALGLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLF 332

Query: 334 FWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKLCP 393
            WTQL+F+LSGM D +LVLRLNLKFG+PDY FIV+D  V ++I RL WM + VL++KLCP
Sbjct: 333 LWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCP 392

Query: 394 RGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKVTRTKFDNLWLAILIRNILRLTPLCM 453
            GIEGTFFALLMSIDN GL+++SW GG +LH+LKVTRT+F NLWLA+L+RN++RL P+C 
Sbjct: 393 HGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKVTRTEFGNLWLAVLVRNVMRLLPICF 452

Query: 454 LFLVPRGDPNSSILLTELPSEEVDHETCEADNIELVSLVNGIN 496
           LFLVP+GD N+  L  E+  E+ + E     N+EL SLV+ ++
Sbjct: 453 LFLVPQGDQNTFKLPAEIMGEDSEEEGTR--NLELASLVDYVD 490

BLAST of MS001365.1 vs. ExPASy Swiss-Prot
Match: Q9LEV7 (Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana OX=3702 GN=At5g10820 PE=2 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 5.8e-118
Identity = 213/461 (46.20%), Postives = 319/461 (69.20%), Query Frame = 0

Query: 34  WFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGITSI 93
           W ++L   ++ SFVLG+V+VYG++QG  G++ +V T+YY KDVQ+VQPS  Q+Y G+  I
Sbjct: 45  WLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFKVVTDYYWKDVQQVQPSVVQLYMGLYYI 104

Query: 94  PWMVKPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAGSA 153
           PW+++PIWGL TD+ PI GY+R+PYFV++G+LG++S +++ +  KL   LA+  L   SA
Sbjct: 105 PWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLGLVSAIAIVVLGKLPAALALSCLLGVSA 164

Query: 154 GVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGVYG 213
            +A+ADV IDAC+A NS    +LA D+QSLC + SS GAL+G++ SG+ VH +G +G  G
Sbjct: 165 AMAIADVVIDACIATNSINIRSLAPDIQSLCMVCSSAGALVGYATSGVFVHQLGPQGALG 224

Query: 214 LLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAG---KAMWATLKCPNVWRPCLYMY 273
           +LA     ++++G  + E        ++  +   G G   K M  T+K P VW+P LYM+
Sbjct: 225 VLAFSPATIVILGFFIYEKRSSTVPTQKTKKDTDGLGVAVKGMCKTVKYPEVWKPSLYMF 284

Query: 274 LSLTLCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRD 333
           +SL L +  +EG FYWYTD   GP FSQE VG I+++G++ S+ G L+Y   LK + FR+
Sbjct: 285 ISLALNISTHEGHFYWYTDPTAGPAFSQEFVGIIYAVGALASMFGVLIYHKKLKGYSFRN 344

Query: 334 LLFWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKL 393
           +LF+ QL++  SGMLD + + R NL  G+PD  F++ +ES  ++I++++W+P++VLS++L
Sbjct: 345 ILFFAQLLYVFSGMLDLVFIKRWNLTLGIPDSLFVITEESFTKMISKIRWIPMVVLSTRL 404

Query: 394 CPRGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKVTRTKFDNLWLAILIRNILRLTPL 453
           CP GIEGTFFA LM ID+ G L++ WGGGF+LH   VTR  F NLWL ILIRNILRL  +
Sbjct: 405 CPLGIEGTFFAFLMCIDSFGQLASKWGGGFVLHAFGVTRHDFGNLWLVILIRNILRLVTV 464

Query: 454 CMLFLVPRGDPNSSILLTE-LPSEEVDHETCEADNIELVSL 491
           C +FLVP  D    ++ ++ LP ++ + +  + D+I+L+ L
Sbjct: 465 CFVFLVPDSDHLDDLVPSDVLPKKQSEDD--DDDDIKLLLL 503

BLAST of MS001365.1 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 4.7e-51
Identity = 142/429 (33.10%), Postives = 232/429 (54.08%), Query Frame = 0

Query: 48  LGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDL 107
           + + +VY +   LG  L R+A  +Y+KD   + P+E  V +G++S+PW+VKP++G ++D 
Sbjct: 128 VAVAMVYFVQGVLG--LARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFISDS 187

Query: 108 VPILGYQRRPYFVLAGLLGVI--SLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDAC 167
           VP+ GY+RR Y VL+GLLG    SL++  +  K      ILL   GS  VA +DV +D+ 
Sbjct: 188 VPLFGYRRRSYLVLSGLLGAFSWSLMAGFVDSKYSAAFCILL---GSLSVAFSDVVVDSM 247

Query: 168 VAQNS-SIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLL 227
           V + +     +++  LQSLC  SS+ G ++    SG LV   G + V+G+ A+   +   
Sbjct: 248 VVERARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTALLPLITSA 307

Query: 228 VGILLNEPHL------PDFNYRQVNEKFVGAGK----AMWATLKCPNVWRPCLYMYLSLT 287
           V +L+NE  +         N   ++  F+   K     +W  +K PNV+ P L+++  L 
Sbjct: 308 VAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPTLFIF--LW 367

Query: 288 LCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFW 347
                ++   +++T +K G  F+ E +G +  + S+ SLLG  LY   LK    R +   
Sbjct: 368 QATPHSDSAMFYFTTNKLG--FTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLRKIFLV 427

Query: 348 TQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRG 407
           T +  +  GM   +LV   N + G+ D +F + D  +  ++ +  +MP+LVL+++LCP G
Sbjct: 428 TTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAARLCPEG 487

Query: 408 IEGTFFALLMSIDNVGLLSASWGGGFLLHILKVTRTKFDNLWLAILIRNILRLTPLCMLF 464
           +E T FA LMSI N G +     G  L     +TR  F NL   I++ N+  L PL +L 
Sbjct: 488 MEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLPLPLLG 547

BLAST of MS001365.1 vs. ExPASy TrEMBL
Match: A0A6J1BS52 (probable folate-biopterin transporter 2 OS=Momordica charantia OX=3673 GN=LOC111005061 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.3e-281
Identity = 501/503 (99.60%), Postives = 502/503 (99.80%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL
Sbjct: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
           LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL
Sbjct: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR
Sbjct: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSL LCLDINEGLFYWYTDSKDGPNFSQEN
Sbjct: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKDGPNFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP
Sbjct: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC
Sbjct: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480

Query: 481 EADNIELVSLVNGINIDNQKEVL 504
           EADNIELVSLVNG+NIDNQKEVL
Sbjct: 481 EADNIELVSLVNGMNIDNQKEVL 503

BLAST of MS001365.1 vs. ExPASy TrEMBL
Match: A0A6J1HDX8 (probable folate-biopterin transporter 2 OS=Cucurbita moschata OX=3662 GN=LOC111462680 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.1e-255
Identity = 457/502 (91.04%), Postives = 475/502 (94.62%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEE+KL    E L EEDEPQKES   IGTA+YWF+ LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEDKLNVFDEDL-EEDEPQKESSVSIGTAVYWFKTLVSEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGL TDLVP+LGY RRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPVLGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALH+KLHLLLAILLLTAGSAGVA+ADVTIDACVAQNSSIHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHQKLHLLLAILLLTAGSAGVAIADVTIDACVAQNSSIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSS+GALLGFSISGILVHLIGSKGVYGLLAIPAGLV LVG++LNEPH+PDFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPAGLVFLVGMILNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW TLK PNVWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWTTLKSPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSL GALLYQYVLKDHQFRDLLFWTQ+I+SLSGMLDFLLVLRLNLK G+P
Sbjct: 301 VGFIFSIGSVGSLFGALLYQYVLKDHQFRDLLFWTQMIYSLSGMLDFLLVLRLNLKIGMP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKF NLWLAILIRN LRLTPLCMLFLVPRGDPNSSILLTEL S +V++ETC
Sbjct: 421 LLHILKVTRTKFSNLWLAILIRNFLRLTPLCMLFLVPRGDPNSSILLTELLSSDVENETC 480

Query: 481 -EADNIELVSLVNGINIDNQKE 502
            EADNIELVSLVNGINIDNQKE
Sbjct: 481 EEADNIELVSLVNGINIDNQKE 501

BLAST of MS001365.1 vs. ExPASy TrEMBL
Match: A0A5A7VCG2 (Putative folate-biopterin transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001930 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 5.3e-255
Identity = 456/502 (90.84%), Postives = 479/502 (95.42%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEEEKLK S E L EEDEPQKES   IG+ALYWF++LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEEKLKISVEDL-EEDEPQKESNVSIGSALYWFKLLVTEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRV+T+YYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGL TDLVPILGY RRPYFV
Sbjct: 61  GGALNRVSTKYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPILGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALHEKLHL+LAILLLTAGSA VA+ADVTIDACVAQNS+IHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHEKLHLVLAILLLTAGSASVAIADVTIDACVAQNSNIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSS+GALLGFSISGILVHLIGSKGVYGLLAIP GLV LVGILLNEPH+PDFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPGGLVFLVGILLNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW+TLK P+VWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWSTLKIPDVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFG+P
Sbjct: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGIP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQL+NRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHIL+VTRTKF NLWLAILIRN+LRL+PLCMLFLVPRGDPNSSIL TELPS EVD++T 
Sbjct: 421 LLHILQVTRTKFSNLWLAILIRNLLRLSPLCMLFLVPRGDPNSSILPTELPSSEVDNDTS 480

Query: 481 -EADNIELVSLVNGINIDNQKE 502
            EADNIELVSLVNG++IDNQKE
Sbjct: 481 EEADNIELVSLVNGMDIDNQKE 501

BLAST of MS001365.1 vs. ExPASy TrEMBL
Match: A0A1S3BC95 (probable folate-biopterin transporter 2 OS=Cucumis melo OX=3656 GN=LOC103488355 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 5.3e-255
Identity = 456/502 (90.84%), Postives = 479/502 (95.42%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEEEKLK S E L EEDEPQKES   IG+ALYWF++LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEEKLKISVEDL-EEDEPQKESNVSIGSALYWFKLLVTEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRV+T+YYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGL TDLVPILGY RRPYFV
Sbjct: 61  GGALNRVSTKYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPILGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALHEKLHL+LAILLLTAGSA VA+ADVTIDACVAQNS+IHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHEKLHLVLAILLLTAGSASVAIADVTIDACVAQNSNIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSS+GALLGFSISGILVHLIGSKGVYGLLAIP GLV LVGILLNEPH+PDFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPGGLVFLVGILLNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW+TLK P+VWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWSTLKIPDVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFG+P
Sbjct: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGIP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQL+NRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLVNRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHIL+VTRTKF NLWLAILIRN+LRL+PLCMLFLVPRGDPNSSIL TELPS EVD++T 
Sbjct: 421 LLHILQVTRTKFSNLWLAILIRNLLRLSPLCMLFLVPRGDPNSSILPTELPSSEVDNDTS 480

Query: 481 -EADNIELVSLVNGINIDNQKE 502
            EADNIELVSLVNG++IDNQKE
Sbjct: 481 EEADNIELVSLVNGMDIDNQKE 501

BLAST of MS001365.1 vs. ExPASy TrEMBL
Match: A0A6J1KA23 (probable folate-biopterin transporter 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492461 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.2e-254
Identity = 456/502 (90.84%), Postives = 473/502 (94.22%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           MVEE+K     E L EEDE QKES   IGTA+YWF+ LV EMHWSFV G+VIVYGISQGL
Sbjct: 1   MVEEDKFNVFDEDL-EEDELQKESSVSIGTAVYWFKALVSEMHWSFVFGVVIVYGISQGL 60

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGL TDLVP+LGY RRPYFV
Sbjct: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLFTDLVPVLGYHRRPYFV 120

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
            AGLLGVISLLSLALH+KLHLLLAILLLTAGSAGVA+ADVTIDACVAQNSSIHPTLAADL
Sbjct: 121 FAGLLGVISLLSLALHQKLHLLLAILLLTAGSAGVAIADVTIDACVAQNSSIHPTLAADL 180

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLCALSSS+GALLGFSISGILVHLIGSKGVYGLLAIPAGLV LVG++LNEPH+PDFNYR
Sbjct: 181 QSLCALSSSIGALLGFSISGILVHLIGSKGVYGLLAIPAGLVFLVGMILNEPHMPDFNYR 240

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVNEKFVGAGKAMW TLK PNVWRPCLYMYLSL LCLDINEGLFYWYTDSK+GP FSQEN
Sbjct: 241 QVNEKFVGAGKAMWTTLKSPNVWRPCLYMYLSLALCLDINEGLFYWYTDSKNGPKFSQEN 300

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFIFSIGSVGSL GALLYQYVLKDHQFRDLLFWTQ+I+SLSGMLDFLLVLRLNLK G+P
Sbjct: 301 VGFIFSIGSVGSLFGALLYQYVLKDHQFRDLLFWTQMIYSLSGMLDFLLVLRLNLKIGMP 360

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF
Sbjct: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           LLHILKVTRTKF NLWLAILIRN LRLTPLCMLFLVPRGDPNSSILLTELP  +VD+ETC
Sbjct: 421 LLHILKVTRTKFSNLWLAILIRNFLRLTPLCMLFLVPRGDPNSSILLTELPRSDVDNETC 480

Query: 481 -EADNIELVSLVNGINIDNQKE 502
            EADNIELVSLVNGINIDNQKE
Sbjct: 481 EEADNIELVSLVNGINIDNQKE 501

BLAST of MS001365.1 vs. TAIR 10
Match: AT5G25050.1 (Major facilitator superfamily protein )

HSP 1 Score: 639.4 bits (1648), Expect = 2.4e-183
Identity = 317/498 (63.65%), Postives = 398/498 (79.92%), Query Frame = 0

Query: 1   MVEEEKLKASAEALEEEDEPQKESGGCIGTALYWFEILVREMHWSFVLGIVIVYGISQGL 60
           +VEE+ L+        + E    +  C    + W ++L  E+HWSFV G+V +YGI+QGL
Sbjct: 2   VVEEQSLENGGSVHVVKGESNFRNVVC--GPVRWLKMLSSELHWSFVFGVVSLYGINQGL 61

Query: 61  GGALNRVATEYYMKDVQKVQPSEAQVYSGITSIPWMVKPIWGLLTDLVPILGYQRRPYFV 120
           GG+L RVATEYYMKDVQKVQPSE+Q  + IT IPW++KP+WG+LTD++PI G+ RRPYF+
Sbjct: 62  GGSLGRVATEYYMKDVQKVQPSESQALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFI 121

Query: 121 LAGLLGVISLLSLALHEKLHLLLAILLLTAGSAGVAVADVTIDACVAQNSSIHPTLAADL 180
           LAG+LGV+SLL ++LH  LHL LA+  +T  SA +A+ADVTIDAC A NS  HP+LA+D+
Sbjct: 122 LAGVLGVVSLLFISLHSNLHLYLALFWMTISSAAMAIADVTIDACTAYNSIKHPSLASDM 181

Query: 181 QSLCALSSSVGALLGFSISGILVHLIGSKGVYGLLAIPAGLVLLVGILLNEPHLPDFNYR 240
           QSLC+LSSS+GALLGF +SGILVHL+GSKGV+GLL  P  LV +VGI+ +EPH+P F+Y+
Sbjct: 182 QSLCSLSSSIGALLGFFMSGILVHLVGSKGVFGLLTFPFALVSVVGIVFSEPHVPGFSYK 241

Query: 241 QVNEKFVGAGKAMWATLKCPNVWRPCLYMYLSLTLCLDINEGLFYWYTDSKDGPNFSQEN 300
           QVN+KF  AGKAMW T+KC +VWRP LYMY+SLTL L+I+EGLFYW+TDSKDGP F+QE 
Sbjct: 242 QVNQKFTDAGKAMWRTMKCSDVWRPSLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQET 301

Query: 301 VGFIFSIGSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVP 360
           VGFI SIGS+GS+L A LYQ VLKDH FR L  WTQL+F+LSGMLD +LV RLNLKFG+P
Sbjct: 302 VGFILSIGSIGSILAATLYQLVLKDHPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLP 361

Query: 361 DYFFIVIDESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGF 420
           DY FIV+DE V Q+I RLKWMPLLVL+SKLCP GIEGTFFALLMSIDN GL+++SW GG 
Sbjct: 362 DYLFIVVDEIVSQMIGRLKWMPLLVLTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGI 421

Query: 421 LLHILKVTRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETC 480
           +LH+LKVTRT+F NLWLA+L+RN++RL PLC LFLVP+GD N+  L  E+  E+ D E  
Sbjct: 422 MLHVLKVTRTEFGNLWLAVLVRNVMRLLPLCFLFLVPKGDQNTFKLPDEIMGEDSDEEKD 481

Query: 481 EAD---NIELVSLVNGIN 496
           E +   N+EL SLV+ ++
Sbjct: 482 EKEGTRNLELASLVHSVD 497

BLAST of MS001365.1 vs. TAIR 10
Match: AT1G79710.1 (Major facilitator superfamily protein )

HSP 1 Score: 571.6 bits (1472), Expect = 6.1e-163
Identity = 275/461 (59.65%), Postives = 365/461 (79.18%), Query Frame = 0

Query: 32  LYWFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGIT 91
           L W  +L+ E+HWSF  G++IVYG+SQGLG  L++V+T+YY KD QK+QPS+AQ+Y G+ 
Sbjct: 35  LRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQYYFKDEQKIQPSQAQIYVGLI 94

Query: 92  SIPWMVKPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAG 151
            IPW++KP+WGLLTD+VP+LGY+RRPYF+LAG L +IS++ L LH  LHL LA+  L AG
Sbjct: 95  QIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISMMVLWLHTNLHLALALSCLVAG 154

Query: 152 SAGVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGV 211
           SAGVA+ADVTIDACV Q S  HPTLAAD+QSLC L SS+G+L+GFS+SG+LVHL+GSKGV
Sbjct: 155 SAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSIGSLVGFSLSGVLVHLVGSKGV 214

Query: 212 YGLLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAGKAMWATLKCPNVWRPCLYMYL 271
           YGLL + AGL+++VG++L E        + VN+KF+ AG A+W T +   VWRPCL+M L
Sbjct: 215 YGLLGVTAGLLVVVGMVLKESPSRSLGRKHVNDKFLDAGSAIWKTFQYGEVWRPCLFMLL 274

Query: 272 SLTLCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDL 331
           S  + L I+EG+FYWYTDSKDGP+FS+E VG I S G++GSL+G LLYQ  LK+  FR++
Sbjct: 275 SAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIMSFGAIGSLVGILLYQNFLKNFPFRNV 334

Query: 332 LFWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKLC 391
           +FW   +  LSG LD +LVLR+NLK G+PDYFFIV+DE V  +I+R+KW+PLLVLSSKLC
Sbjct: 335 VFWALSLSVLSGFLDLILVLRINLKLGLPDYFFIVVDEFVSHMISRIKWLPLLVLSSKLC 394

Query: 392 PRGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKVTRTKFDNLWLAILIRNILRLTPLC 451
           P G+EGTFFALLMSI+++G L +SWGGG LLH LKVTRT+FDNLWL I+IR++LR+ P+ 
Sbjct: 395 PAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHALKVTRTQFDNLWLVIVIRSLLRVIPIG 454

Query: 452 MLFLVPRGDPNSSILLTELPSEEVDHETCEADNIELVSLVN 493
           ++FL+P  D NS+IL  E+ +  +  E  + + IE+ +L++
Sbjct: 455 LVFLIPNVDSNSTILPAEMLTSRIS-EGVDTEKIEMTALIS 494

BLAST of MS001365.1 vs. TAIR 10
Match: AT5G25040.2 (Major facilitator superfamily protein )

HSP 1 Score: 535.4 bits (1378), Expect = 4.9e-152
Identity = 273/488 (55.94%), Postives = 357/488 (73.16%), Query Frame = 0

Query: 34  WFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGITSI 93
           W ++L  ++HWS+V  +V   GI+QG G +L  VAT+YYMKDVQKVQPS+ Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAGS 153
            W++ KP++G+LTD++PI G+ RRPYF+LAG++GV+SLL ++L   LHL LA+  +T  S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGVY 213
           A +A+ADVTIDAC   NS+ HP+LA+D+QSLC+LS S+G LLGF +SGILVHL+GSKGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAGKAMWATLKCPNVWRPCLYMYLS 273
           GLL     LV +VG+L +EP +  F+++Q    F  A KAMW T+KC +VW+P LYM+++
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQ---NFTNAMKAMWRTIKCSDVWQPSLYMFIT 272

Query: 274 LTLCLDINEGLFYWYTDSKDGPNFS-------------------------QENVGFIFSI 333
             L LDI EGLFYW+TDSKDGP F+                         QE VGFI SI
Sbjct: 273 RALGLDIKEGLFYWFTDSKDGPLFAQVIPKQVASIFSKSTNMLKAFLVCFQETVGFILSI 332

Query: 334 GSVGSLLGALLYQYVLKDHQFRDLLFWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVI 393
           GS+GS+LG LLY   LKDH FR L  WTQL+F+LSGM D +LVLRLNLKFG+PDY FIV+
Sbjct: 333 GSIGSILGVLLYNLRLKDHPFRKLFLWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVV 392

Query: 394 DESVHQLINRLKWMPLLVLSSKLCPRGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKV 453
           D  V ++I RL WM + VL++KLCP GIEGTFFALLMSIDN GL+++SW GG +LH+LKV
Sbjct: 393 DGIVSKMIIRLTWMVIFVLNTKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKV 452

Query: 454 TRTKFDNLWLAILIRNILRLTPLCMLFLVPRGDPNSSILLTELPSEEVDHETCEADNIEL 496
           TRT+F NLWLA+L+RN++RL P+C LFLVP+GD N+  L  E+  E+ + E     N+EL
Sbjct: 453 TRTEFGNLWLAVLVRNVMRLLPICFLFLVPQGDQNTFKLPAEIMGEDSEEEGTR--NLEL 512

BLAST of MS001365.1 vs. TAIR 10
Match: AT5G25040.3 (Major facilitator superfamily protein )

HSP 1 Score: 487.6 bits (1254), Expect = 1.2e-137
Identity = 240/399 (60.15%), Postives = 310/399 (77.69%), Query Frame = 0

Query: 34  WFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGITSI 93
           W ++L  ++HWS+V  +V   GI+QG G +L  VAT+YYMKDVQKVQPS+ Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAGS 153
            W++ KP++G+LTD++PI G+ RRPYF+LAG++GV+SLL ++L   LHL LA+  +T  S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGVY 213
           A +A+ADVTIDAC   NS+ HP+LA+D+QSLC+LS S+G LLGF +SGILVHL+GSKGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAGKAMWATLKCPNVWRPCLYMYLS 273
           GLL     LV +VG+L +EP +  F+++Q    F  A KAMW T+KC +VW+P LYM+++
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQ---NFTNAMKAMWRTIKCSDVWQPSLYMFIT 272

Query: 274 LTLCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLL 333
             L LDI EGLFYW+TDSKDGP F+QE VGFI SIGS+GS+LG LLY   LKDH FR L 
Sbjct: 273 RALGLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLF 332

Query: 334 FWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKLCP 393
            WTQL+F+LSGM D +LVLRLNLKFG+PDY FIV+D  V ++I RL WM + VL++KLCP
Sbjct: 333 LWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCP 392

Query: 394 RGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKVTRTK 432
            GIEGTFFALLMSIDN GL+++SW GG +LH+LK  RT+
Sbjct: 393 HGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKEIRTR 428

BLAST of MS001365.1 vs. TAIR 10
Match: AT5G25040.1 (Major facilitator superfamily protein )

HSP 1 Score: 484.6 bits (1246), Expect = 9.8e-137
Identity = 239/396 (60.35%), Postives = 308/396 (77.78%), Query Frame = 0

Query: 34  WFEILVREMHWSFVLGIVIVYGISQGLGGALNRVATEYYMKDVQKVQPSEAQVYSGITSI 93
           W ++L  ++HWS+V  +V   GI+QG G +L  VAT+YYMKDVQKVQPS+ Q  S IT I
Sbjct: 33  WLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAITRI 92

Query: 94  PWMV-KPIWGLLTDLVPILGYQRRPYFVLAGLLGVISLLSLALHEKLHLLLAILLLTAGS 153
            W++ KP++G+LTD++PI G+ RRPYF+LAG++GV+SLL ++L   LHL LA+  +T  S
Sbjct: 93  SWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMTISS 152

Query: 154 AGVAVADVTIDACVAQNSSIHPTLAADLQSLCALSSSVGALLGFSISGILVHLIGSKGVY 213
           A +A+ADVTIDAC   NS+ HP+LA+D+QSLC+LS S+G LLGF +SGILVHL+GSKGV+
Sbjct: 153 AAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSKGVF 212

Query: 214 GLLAIPAGLVLLVGILLNEPHLPDFNYRQVNEKFVGAGKAMWATLKCPNVWRPCLYMYLS 273
           GLL     LV +VG+L +EP +  F+++Q    F  A KAMW T+KC +VW+P LYM+++
Sbjct: 213 GLLTFTFALVSVVGVLFSEPRVHGFSFKQ---NFTNAMKAMWRTIKCSDVWQPSLYMFIT 272

Query: 274 LTLCLDINEGLFYWYTDSKDGPNFSQENVGFIFSIGSVGSLLGALLYQYVLKDHQFRDLL 333
             L LDI EGLFYW+TDSKDGP F+QE VGFI SIGS+GS+LG LLY   LKDH FR L 
Sbjct: 273 RALGLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLF 332

Query: 334 FWTQLIFSLSGMLDFLLVLRLNLKFGVPDYFFIVIDESVHQLINRLKWMPLLVLSSKLCP 393
            WTQL+F+LSGM D +LVLRLNLKFG+PDY FIV+D  V ++I RL WM + VL++KLCP
Sbjct: 333 LWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCP 392

Query: 394 RGIEGTFFALLMSIDNVGLLSASWGGGFLLHILKVT 429
            GIEGTFFALLMSIDN GL+++SW GG +LH+LK T
Sbjct: 393 HGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKNT 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132109.18.9e-28199.60probable folate-biopterin transporter 2 [Momordica charantia][more]
XP_038885031.11.2e-25691.85probable folate-biopterin transporter 2 [Benincasa hispida][more]
KAG7029472.15.8e-25691.24putative folate-biopterin transporter 2 [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_023547193.19.9e-25691.24probable folate-biopterin transporter 2 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022962138.16.4e-25591.04probable folate-biopterin transporter 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q5FV413.4e-18263.65Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g2... [more]
Q9SQN28.6e-16259.65Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana OX=3702 GN=At1g7... [more]
F4KIL84.6e-15558.96Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana OX=3702 GN=At5g2... [more]
Q9LEV75.8e-11846.20Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana OX=3702 GN=At5g1... [more]
Q9SKZ54.7e-5133.10Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1BS524.3e-28199.60probable folate-biopterin transporter 2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1HDX83.1e-25591.04probable folate-biopterin transporter 2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5A7VCG25.3e-25590.84Putative folate-biopterin transporter 2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BC955.3e-25590.84probable folate-biopterin transporter 2 OS=Cucumis melo OX=3656 GN=LOC103488355 ... [more]
A0A6J1KA231.2e-25490.84probable folate-biopterin transporter 2 isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
AT5G25050.12.4e-18363.65Major facilitator superfamily protein [more]
AT1G79710.16.1e-16359.65Major facilitator superfamily protein [more]
AT5G25040.24.9e-15255.94Major facilitator superfamily protein [more]
AT5G25040.31.2e-13760.15Major facilitator superfamily protein [more]
AT5G25040.19.8e-13760.35Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039309Biopterin transporter familyPFAMPF03092BT1coord: 58..225
e-value: 8.2E-41
score: 139.8
coord: 266..460
e-value: 2.2E-55
score: 187.9
IPR039309Biopterin transporter familyPANTHERPTHR31585FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTICcoord: 9..495
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 43..441
e-value: 2.4E-24
score: 87.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 46..457
IPR004324Folate-biopterin transporterTIGRFAMTIGR00788TIGR00788coord: 33..459
e-value: 2.9E-67
score: 225.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR31585:SF49FOLATE-BIOPTERIN TRANSPORTER 2-RELATEDcoord: 9..495
NoneNo IPR availableCDDcd17484MFS_FBTcoord: 49..454
e-value: 1.44613E-146
score: 423.946

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS001365MS001365gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS001365.1-cdsMS001365.1-cds-scaffold36:3581626..3582240CDS
MS001365.1-cdsMS001365.1-cds-scaffold36:3582453..3582623CDS
MS001365.1-cdsMS001365.1-cds-scaffold36:3582694..3582789CDS
MS001365.1-cdsMS001365.1-cds-scaffold36:3582892..3583151CDS
MS001365.1-cdsMS001365.1-cds-scaffold36:3583811..3584177CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS001365.1MS001365.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane